Shimadzu HPLC Columns

C190-E043CShimadzu HPLC Columns Shimadzu High Per for mance Liquid Chromatograph 2 CONTENTS 1 Classification of Shimadzu HPLC Columns Classification by Support Material ………………… 4 Classification by Elution Mode …………………… 5 Classification by Column Dimensions …………… 6 Classification by ODS Columns …………………… 7 2 Guide for Selection of Operational Conditions Amino Acids ………………………………………… 8 Peptides and Proteins ……………………………… 9 Nucleic Acids ……………………………………… 10 Sugars ……………………………………………… 11 Vitamins …………………………………………… 12 Carboxylic acids …………………………………… 13 Ion …………………………………………………… 14 3 Shimadzu HPLC Columns Shim-pack Shim-pack VP-ODS for GLP/GMP compliance … 15 Shim-pack HT-ODS ……………………………… 19 Shim-pack FC-ODS ……………………………… 20 Shim-pack HRC, MRC, PRC……………………… 21 Shim-pack CLC …………………………………… 24 Shim-pack PREP ………………………………… 28 Shim-pack FLC, SBC, MBC ……………………… 30 Shim-pack Ion Exchange Columns ……………… 33 Shim-pack GPC …………………………………… 38 Shim-pack Columns for Gel Filtration/Ion Exclusion Chromatography …………………………………… 41 Shim-pack IC ……………………………………… 45 Shim-pack HPC …………………………………… 47 Shim-pack SPC …………………………………… 49 Shim-pack GRD, Pre-column …………………… 50 Dedicated Shim-pack Columns ………………… 51 Shim-pack Preparative Columns ………………… 53 Shim-pack BIO (T) ………………………………… 57 Asahipak® Asahipak® ODS …………………………………… 59 Asahipak® GS ……………………………………… 62 Asahipak® ES ……………………………………… 64 Asahipak® NH2P …………………………………… 65 CAPCELL PAK …………………………………… 66 …………………………………… 69 Zorbax STR STR ODS Series …………………………………… 70 4 Application Data …………………………… 73 5 Parts for Flow line Plumbing parts (Connecting Parts) ……………… 83 PEEK plumbing parts ……………………………… 85 Shimadzu Amino Acid Analysis kit for amino acid analytical system …………………………………… 87 Disposable Filter …………………………………… 88 6 Shim-pack Columns ……………………… 90 7 Sample and data …………………………… 98 3 Silica Gel Type High mechanical strength. GPC) Polyacrylate Shim-pack Series(PA. SCR. TMS. NH2. NH2. C8. ION. ISA.1 Classification of Shimadzu HPLC Columns ■Classification by Support Material The solid supports of packing materials for HPLC are classified into the following two groups with the following features. TMS. CN. Phenyl.) STR Series(ODS) Shimadzu HPLC Columns Polymer Type Polystyrene Shim-pack Series (Amino. etc. SIL) CAPCELL PAK Series (C18. ODP) . IC) Polyvinyl alcohol 4 Asahipak Series(GS. C8) Silica Gel Type Zorbax Series(ODS. IC. ES. C8. SIL. High efficiency Packing Materials for HPLC Polymer Type High stability in wide pH range The Shimadzu HPLC columns can be classified into two groups as follows: Shim-pack Series (ODS. hence easily pulverized into very fine particles. CN. ISC. Zorbax. Zorbax) ……Steroid hormones ODS(C18)(Shim-pack. Zorbax.proteins Shim-pack WCX(Weakly acidic cation exchange) ……Proteins Ion exchange Zorbax SAX(Weakly basic anion exchange)……Nucleotides Zorbax SCX(Weakly acidic cation exchange)……Catecholamines Gel filtration Shim-pack Diol……Proteins Hydrophobic Interaction Chromatography Shim-pack HPC……Proteins. 102H(Sulfonated polystyrene)……Organic acids Gel Filtration Asahipak GS(Polyvinyl alcohol)……Water-soluble high polymers GPC Shim-pack GPC(Polystyrene)……Synthetic high polymers. Zorbax) Low molecular-weight compounds TMS(Shim-pack. nucleic acids Affinity Chromatography Shim-pack AFC……Proteins. nucleic acids Asahipak ES(Polyvinyl alcohol)……Proteins Ligand exchange Shim-pack SCR(Sulfonated polystyrene)……Saccharides Ion exclusion Shim-pack SCR-101H. proteins. Zorbax)……Phospholipids Normal phase elution NH2(Shim-pack. tocopherols CN(Shim-pack.■Classification by Elution Mode ● The two groups are futher classified as follows: SIL(Shim-pack. ISC(Polystyrene)……Amino acids. rubbers 5 . Zorbax) ……Saccharides. CAPCELL PAK. Zorbax) Phenyl(Shim-pack) Shim-pack WAX(Weakly basic anion exchange) ……Nucleotides. Polystyrene)……Inorganic ions Shim-pack PA(Polyacrylate)……Proteins. guanidines Shim-pack ISA(Polystyrene)……Saccharides Polymer Type Ion exchange Shim-pack IC(Polyacrylate. low-molecular-weight compounds Shim-pack Amino. STR) C8(Shim-pack. CAPCELL PAK) Silica Gal Type Reversed phase elution CN(Shim-pack. nucleic acids Reversed phase elution Asahipak ODP-50(Polyvinyl alcohol)……Peptides. 5mm i. ●Though this series columns are compatible with an ordinary HPLC.d.d. column. ● The Small bore columns ● Shimadzu SBC series small bore columns are 2. because the same linear velocity can be obtained with about 1/20 flow rate. 30mm i. ● Column packing is only 5cm long. Each is 25cm long.6mm i.d.d.×15cm or 25cm CLC(M) series 6.d. and 15∼30cm long columns are most widely used and are available in a wide variety. and connecting pipes of a small capacity must be used..×15cm FLC series Small bore 2.d.26) Micro bore columns ● Shimadzu MBC series micro bore columns are only 1. ●The sample injector. the detector flow-thru cell. 6 .0mm i.d. ● The Fast-LC columns ● Shimadzu FLC series fast LC columns are packed with 3μm dia.6mm i.0mm in inner diameter.×25cm or 50cm MBC series Semi-preparative Preparative column Large scale preparative HPLC columns Standard Analytical column Fast analysis Moble phase consumption is less than 1/3＊ Moble phase consumption is less than 1/20＊ ＊ Compared with column 4.×15cm SBC series Micro bore 1. column. 50mm i.d.d.d.0mm i. ● As standard size analytical columns.0mm i.■Classification by Column Dimensions ● HPLC columns are classified as analytical columns and preparative columns according to the dimensions.d. it is better to use small capacity units.×25cm PREP(L) series 4. ● They are compatible with an ordinary HPLC and permit very fast analysis. (Refer to P. Micro bore columns and small bore columns are available.5mm in inner diameter.×30cm GPC series Fast-LC 4.6mm i. (Column size) (Representative Shim-pack series) 20mm i. ● You can choose 20mm i. mobile phase consumption is only about 1/20 that required in a 4.6mm i.×15cm HRC.d. analytical columns such as Fast-LC columns.CLC series 8.d. ● Analytical columns are further classified into several groups.×25cm PREP(K) series 50mm i. preparative columns in accordance with the scale of preparative.d.d.×15cm or 25cm ISC series etc.×25cm PREP series 30mm i. 4.d. ● As other special sizes.. mobile phase consumption is about 1/3 compared with a 4.d.0mm i.6mm i. 4∼8mm i. Shimadzu provides various types of ODS columns to ensure a wide field of application.■Classification by ODS Columns ODS columns ODS columns are packed with packing material having octadecyl group chemically-bonded on the surface and are most popularly used in HPLC. Asahipak ODP. STR ODS. 2∼10 Asahipak ODP-50 Applicable to mobile phase up to pH13. -ODS(K) Preparative STR ODS Analysis for general use CAPCELL PAK C18 Analysis for general use Polymer-coated and end-capped Zorbax ODS Analysis for general use Non end-capped Asahipak ODS Analysis for general use Polyvinyl alcohol gel t o i nt roduc e C18 End-capped Method to Introduce C18 Groups Selection of ODS Columns As standard analytical columns. Silica Gel Type Shimadzu HPLC ODS Columns Polymer Type Shim-pack HRC-ODS Analysis for general use Shim-pack CLC-ODS Analysis for general use Shim-pack FLC-ODS Fast-LC Shim-pack MBC-ODS Micro bore LC Shim-pack SBC-ODS Small bore LC Shim-pack PREP-ODS. 2∼7. according to the state of end-capping and the characteristics of the silica gel used as the solid support. Column name Features Mobile phase pH range Shim-pack HRC-ODS Shim-pack CLC-ODS STR ODS End-capped to minimize the influence of residual silanol groups and eliminate tailing of basic compounds. CLC-ODS. The following table is a rough guide for selecting ODS columns. -ODS(H). They can also be different with the brand.5 CAPCELL PAK C18 Silica gel solid support is coated with silicone polymer to improve stability in basic mobile phase. 2∼7. CAPCELL PAC C18.5 Zorbax ODS Utilizes the influence of residual silanol compounds. Zorbax ODS. we provide Shim-pack HRC-ODS. The selectivity is different from that of silica gel types. -ODS(L). The delicate differences in separation properties can be utilized to achieve some difficult analysis. 2∼13 7 . ODS columns can have different separation properties. The representative reagents used in the post-column derivation method are OPA (for fluorescing) and ninhydrin. Special accessories are not needed. Combination of the post-column derivatization method and the cation exchange method permits analysis of wide variety of amino acids ranging from free amino acids to amino acids produced in hydrolysis of proteins. and basic according to the number of these groups. The reagents for pre-column derivatization such as OPA(ortho-phthalaldehyde) are commercially available.2 Guide for Selection of Operational Conditions ■Amino Acids Amino acids are amphoteric compounds having carboxyl groups and amino groups. Derivatization method ● Pre-column derivatization method: The sample solution is derivatized before entering the analytical column. neutral. depending on the group at the position of R. ● Reversed Detection Method ● The UV absorption method which utilizes the absorption of carboxyl groups at 200∼210nm is not widely used though it is useful for detecting some type of amino acids. Separation mode ● The ● It cation exchange chromatography is most popular. Also they are classified as aliphatic type and aromatic type. Post-column method Continuously add reaction solution to column elution. Amino acids are classified as acidic. Li type and Na type. ● Post-column deribatization method: The derivatizing reagent is continuously added to the column effluent. ● It is quite effective for separating amino acids derivatized by precolumn derivatization. etc. This method has the noteworthy advantage that the entire operation can be easily automatized. is classified into two methods. It is also used for separating glutamine and glutamic acid. Combination of the pre-column derivatization method and the reversed phase elution mode provides rapid analysis of amino acids produced in hydrolysis of proteins. ● Pre-column derivatization and post-column derivatization methods are often used for detecting amino acids. Column (Sample) (Separation mode) (Typical column) (Detection) Shim-pack Amino-Li Free amino acids Amino Acids Cation exchange Cation exchange Post-column derivation and fluorescing with OPA Shim-pack Amino-Na Protein-hydrolyzed amino acids Reversed-phase Shim-pack HRC-ODS CAPCELL PAK C18. Ease of automation and suited for routine analysis. Li type Biological amino acids Na type Protein-hydrolyzed amino acids Cation exchange ● The Li type is recommended for analysis of biological amino acids. phase elution mode can separate particular type of amino acids but cannot separate all free amino acids. Pre-column derivatization and fluorescing with OPA Shimadzu HPLC Amino Acid Analysis System Application Data Book (C190-E004) 8 . Pre-column method React before injects in columns. 23) Asahipack ODP-50 (Refer to P. ● Detection Column (Separation mode) Gel filtration (Pepresentative column) Shim-pack Diol (Refer to P. Their ionic and hydrophobic properties are different depending on the type and the number of constituting amino acids.64) Shim-pack WCX-2 Peptides Proteins Shim-pack PA-DEAE Anion exchange Reversed phase Hydrophobic (Refer to P.62) Shim-pack WCX-1 Shim-pack PA-CM Cation exchange (Refer to P. Proteins have high-degree structures with various combinations. at about 210nm is used for detection of peptides consisting of aliphatic amino acids and detection of lowconcentration peptides and proteins.64) Shim-pack HRC-ODS (Refer to P.41) Asahipak GS (Refer to P.66) STR ODS (Refer to P.47) 9 . Gel filtration Separation by molecular sizes Ion exchange Separation by molecular electric charge Reversed phase Separation by molecular hydrophobic properties Hydrophobic Detection Method ● The UV spectrophotometric method is generally used.■Peptides and Proteins Peptides and proteins consist of amino acids which are combined by acid-amide bonding.21) Shim-pack CLC-ODS (Refer to P.70) Shim-pack HPC (Refer to P. Detection at 280nm is effective in most cases.33) Shim-pack PA-QA Asahipack ES-502N (Refer to P. Separation mode ● Peptides and proteins are generally analyzed by one of the following methods.33) Shim-pack PA-SP Asahipack ES-502C (Refer to P.59) CAPCELL PAK C18 (Refer to P. 62) (Refer to P. GS (Refer to P.33) Nucleic acid bases and nucleosides Nucleic Acids Nucleotides Reversed phase Gel filtration Anion exchange DNA and RNA Hydrophobic 10 Shim-pack HRC-ODS Shim-pack CLC-ODS Asahipak GS Shim-pack Diol Asahipak GS Shim-pack WAX-2 Shim-pack PA-DEAE Shim-pack PA-QA Asahipak ES-502N Shim-pack HPC (Refer to P. therefore UV spectrophotometric detection (250∼260nm) is used in most cases. DNA.64) (Refer to P. ● Nucleotides Detection Method ● Nucleic acids and related compounds generally have strong ultraviolet absorption. Column (Separation mode) (Column) Cation exchange Shim-pack WCX-1 (Refer to P.33) (Refer to P.14) (Refer to P. gel filtration mode. ● In analysis of high molecular weight samples such as oligonucleotides. and RNA.59) Anion exchange Shim-pack WAX-1 (Refer to P.41) (Refer to P.62) (Refer to P.16) (Refer to P.24) Reversed phase Shim-pack HRC-ODS Shim-pack CLC-ODS Asahipak ODP-50. and ion exchange mode are selectively used.■Nucleic Acids Nucleic acids play important roles in organism. are analyzed in the anion exchange mode in most cases. They are classified into the following three groups: Nucleic acid base Nucleoside (Nucleic acid base ＋ sugar) Nucleotide (Nucleoside ＋ phosphorus) Separation mode ● Nucleic acid bases and nucleosides are analyzed in the cation exchange mode or the reversed phase elution mode. hydrophobic mode.14) (Refer to P.30) (Refer to P. The reversed phase elution is also useful with suitable selection of mobile phases.47) . etc.65) Oligosaccharides Polysaccharides Gel filtration 11 . fructose.■Sugars Sugars such as glucose and sucrose are very common and are available in a great variety. etc.~15-saccharide Poor separation of epimers Ligand exchange Separation by the interaction between and counter-ion on sulfonic acids Separation of monosaccharides and separation of sugars from alcohols Poor separation of diand higher saccharides Anion exchange Separation by the interaction based on ion exchange of sugar-borate complex Separation of mono-~trisaccharides Time consuming Separation by the size of the molecular Measurement of molecular weight distribution Components of the same molecula weight cannot be separated.62) Mono~ trisaccharides Partition (Refer to P. Monosaccharides Sugars Separation mode ● Sugars are generally analyzed by one of the following methods. refractive index detector is generally used in the analysis of sugars.24) (Refer to P.33) Shim-pack HRC-NH2 Shim-pack CLC-NH2 Asahipak NH2P (Refer to P.41) Shim-pack ISA-07 (Refer to P. arabinose. Polysaccharides Polymers consisting of many monosaccharides Starch. Hexose (six carbon atoms per molecule) Glucose. cellulose. etc. Disaccharides combination of two monosaccharides Sucrose. Oligosaccharides combination of 2~10 monosaccharides Dextrin. as in the case of brewage products and natural materials. the postcolumn derivatization method is used. etc. maltose.21) Asahipak GS (Refer to P. galactose. ● When sugar content is low and impurity content is high. Partition Separation by the interaction based on partition Separation of mono. Pentose (five carbon atoms per molecule) Xylose. Gel filtration Detection Method ●A UV spectrophotometric detector is not used because sugars absorb radiation only around 190nm. etc. ●A Column Monosaccharides (Separation mode) Ligand exchange Anion exchange Sugars (Column) Shim-pack SCR-101N Shim-pack SCR-101C Shim-pack SCR-101P (Refer to P. Em.■Vitamins Vitamins are classified as water-soluble type and oil-soluble type. B6 (pyridoxine).24) Shim-pack HRC-ODS Shim-pack CLC-ODS STR-ODSH Shim-pack HRC-NH2 Shim-pack CLC-NH2 (Refer to P. nicotinic acid. 340nm.21) (Refer to P.24) .21) (Refer to P. B1 (thiamine).21) (Refer to P. nicotinamide Vitamin B Water-soluble vitamins Vitamin C Ascorbic acid Vitamin H Biotin acid Vitamin M Folic acid Vitamin P Hesperidin Pantothenic acid Vitamin A Retinol Vitamin D Oil-soluble vitamins D2 (ergocalciferol). Column (Separation Mode) Water-soluble vitamins Reversed phase (Ion-pair) Partition (Vitamin C) Vitamins Reversed phase Oil-soluble vitamins Partition (Vitamin E isomers) 12 Shim-pack HRC-ODS Shim-pack CLC-ODS STR-ODSH (Refer to P.70) (Refer to P. B12 (cyanocobalamine). BT (carnitine). they can be influenced by the state of mobile phase. UV (325nm) Vitamin A (retinol) Fluo (Ex.24) (Refer to P. 295nm.24) (Refer to P. 460nm) Vitamin B1 (thiamin) UV (270nm) Vitamin B2 (riboflavin) UV (270nm) Vitamin B6 (pyridoxine) UV (290nm) Vitamin B12 (cyanocobalamine) UV (280nm) UV (210nm) Vitamin BT (carnitine) RI Vitamin C (ascorbic acid) UV (245nm) Vitamin D UV (265nm) UV (295nm) Vitamin E (tocopherol) Fluo (Ex. B2 (riboflavin).70) Shim-pack HRC-NH2 Shim-pack CLC-NH2 (Refer to P. ● Some Detection Method ● The UV spectrophotometric method is generally used: the spectrofluorometric method is used for some vitamins. D3 (cholecalciferol) Vitamin E Tocopherol Vitamin K Separation mode ● Both water-soluble and oil-soluble vitamins are analyzed in the reversed phase elution mode.21) (Refer to P. oil-soluble vitamins are analyzed in the partition mode. Em. 325nm) Vitamin H (biotin) UV (210nm) Vitamin K UV (250nm) Vitamin M (folic acid) UV (280nm) Vitamin P (hesperidin) UV (265nm) Note: The above wavelength values are approximate. etc. etc. Saturation aliphatic carboxylic acids Non saturation aliphatic carboxylic acids Acetate. therefore UV spectrophotometoric detector can be used. Separation mode Ion exclusion chromatography. malic acid. anion ion exchange chromatography and reversed phase chromatography can be used in accordance with the dissociation and the side chain of carboxylic acid. etc. mandelic acid. Carboxylic acids Benzuic acid. ● Post-column conductivity detector making use of separating carboxul group makeks it possible to improve selectivity. citric acid. poly (more than 3) carboxylic acids in accordance with the number of carboxyl group. Acrylic acid. As a result. Column Saturation aliphatic carboxylic acids Reversed phase Shim-pack HRC-ODS Shim-pack CLC-ODS STR ODS etc. Therefore. ● Ion exclusion chromatography is suitable for the analysis of high aqueous carboxylic acids and used widely to analyze food and cultivation solutions. etc. pre-column derivatization using label to carboxyl group might be used. Detection Method ● Carboxylic acids have slight absorbency at around 205nm. ● However. including carboxyl group and stand for acids properties. di.■Carboxylic acids Carboxylic acids are organic compounds. These are classified as follows. oxalacetic acid. Keto carboxylic acids Pyruvic acid. these absorbencies are relatively strong so that can be detected with a high sensitivity. ● Anion exchange chromatography is a basic mode of carboxylic acids having the properties of acids and has an advantage of separating from neutral components. the absorbency coefficients of carboxylic acids are generally small and the selectivity of the detection are poor. Aliphatic carboxylic acids Hydroxy carboxylic acids Lactic acid. Aromatic carboxylic acids Carboxylic acids are also classified into mono. Ion exclusion Shim-pack SCR-102H Shim-pack SCR-101H Non saturation aliphatic carboxylic acids Aliphatic carboxylic acids Hydroxy carboxylic acids Carboxylic acids Keto carboxylic acids Aromatic carboxylic acids Anion exchange Shim-pack WAX-1 Shim-pack IC-A1 Reversed phase Shim-pack HRC-ODS Shim-pack CLC-ODS STR ODS 13 . ● Reversed phase chromatography is suitable for the separation of fatty acid and aromatic carboxylic acids. linoleic acid. As far as aromatic carboxylic acids and aliphatic carboxylic acids are concerned. there are cases where it is difficult to analyze some samples. etc. fumaric acid. stearic acid. succinic acid. Silicate L-ECD 2− NO− 4 . (Detection Method) (Analytical objection) Inorganic ions CDD Post-column pH UV VIS Carboxylic acids Indirect absorbance method Inorganic ions Direct Absorbance method UV absorbance ion − − − − NO 2 .24) Ion Reversed phase Shimadzu Ion Chromatography System Application DataBook (C190-E002) 14 .41) Shim-pack HRC-ODS Shim-pack CLC-ODS (Refer to P.■Ion Ions are classified as follows. many selectible detector can be used to make use of each chemical property. special ions. Rare-earth metals RI Boricacid. NO 3 Br . generally conductivity detector can be used with a high sensitive.21) (Refer to P. I Carboxylic acid Post-column method Transition metals. but sometimes ion pair and reversed phase method are used.24) Ion exclusion Shim-pack SCR-101H Shim-pack SCR-102H (Refer to P. Mainly inorgainic ion and low molecale organic ion are the object of ion chromatography analysis. Organic ion Ion Anion ion Carboxylic acids Sulfonic acids Cation ion Amine Amphoteric ion Amino Acide Anion ion Inorganic anion ion Cation ion Alkaline metals Alkaline-earth metals Transition metals Rare-earth metals Inorganic ion Separation mode Ion exchange is a main method. Detection Method ● In ● In IC (Ion chromatography) analysis. SO 4 etc.21) (Refer to P. RF Post-column method NH＋ 4 Column (Separation mode) Ion exchange (Representative column) Shim-pack IC (Refer to P.45) Ion pair Shim-pack HRC-ODS Shim-pack CLC-ODS (Refer to P. surface treatment and packing 228-34937-91 228-34937-92 4. No.0×250 4. 〈VP-ODS Series〉 Excellent manufacturing uniformity ■Analytical Column Shim-pack VP-ODS Cat.0×150 customers. 2.D. a set of 3pcs. it would 228-34937-95 228-34937-96 228-34937-97 2. N-acetylprocainamide ２. I. ＊An analytical column set consists of three columns whose packings (Silica-based) are from different production batches. For development or validation of method.D.6×250 procedures are strictly controlled respectively and only the 228-34937-93 228-34937-94 6. a set of 3pcs. I. manufacturing uniformity of columns is increasing.Shim-pack 3 Shimadzu HPLC Columns Shim-pack VP-ODS for GLP/GMP compliance For development or validation of analytical method.6×150.D.6×150 4. 228-36849-91 228-36849-92 228-36849-93 I. Shim-pack VP-ODS ■Guard Column Shim-pack VP-ODS Cat. 228-34938-94 A holder for I.6mm 228-34938-93 2. 228-34938-92 A holder for I. exchangeable cartridge 2pcs.×length (mm) To minimize column-to-column performance deviation of ODS columns. silica-bases.×length (mm) Remarks 4. Holders can be used repeatedly. No.0×150. 2. ■Short Columns for Analytical/Preparative Cat.×length (mm) 228-34938-91 4. exchangeable cartridge 2pcs. 4. products that passed the quality criteria are delivered to be efficient to run the test with a set of three columns with packings of different batches.6×10 Incl. Shim-pack VP-ODS has been developed to meet such expectations.0×150 2.D. 1 Comparison of lot-to-lot repeatability (Silica-based material) 15 .D. Phenol Batch B Batch A Fig.0mm Batch C A company's column Batch B Batch A ＊Guard column consists of a cartridge and holder.0×5 Incl.6×50 5μm diameter. No. 20×50 12nm (120Å) pore 20×100 porous silica ODS Batch C ■Peaks １. No.6×10 Incl.D. N-acetylprocainamide ２.×length (mm) 228-34938-91 4. ＊An analytical column set consists of three columns whose packings (Silica-based) are from different production batches. 228-36849-91 228-36849-92 228-36849-93 I.0×5 Incl. 228-34938-94 A holder for I.6×150 4.0×150 customers.0mm Batch C A company's column Batch B Batch A ＊Guard column consists of a cartridge and holder. I. I. a set of 3pcs. a set of 3pcs. exchangeable cartridge 2pcs.6×150. 20×50 12nm (120Å) pore 20×100 porous silica ODS Batch C ■Peaks １.D. 1 Comparison of lot-to-lot repeatability (Silica-based material) 15 . Phenol Batch B Batch A Fig.×length (mm) Remarks 4. Holders can be used repeatedly.×length (mm) To minimize column-to-column performance deviation of ODS columns. surface treatment and packing 228-34937-91 228-34937-92 4. products that passed the quality criteria are delivered to be efficient to run the test with a set of three columns with packings of different batches. 2. silica-bases. For development or validation of method.D. Shim-pack VP-ODS has been developed to meet such expectations.0×150 2. ■Short Columns for Analytical/Preparative Cat.Shim-pack 3 Shimadzu HPLC Columns Shim-pack VP-ODS for GLP/GMP compliance For development or validation of analytical method.D. 4. 2.0×150.6mm 228-34938-93 2.0×250 4.6×50 5μm diameter. 228-34938-92 A holder for I. 〈VP-ODS Series〉 Excellent manufacturing uniformity ■Analytical Column Shim-pack VP-ODS Cat.6×250 procedures are strictly controlled respectively and only the 228-34937-93 228-34937-94 6. No. manufacturing uniformity of columns is increasing. No. Shim-pack VP-ODS ■Guard Column Shim-pack VP-ODS Cat. exchangeable cartridge 2pcs.D. it would 228-34937-95 228-34937-96 228-34937-97 2. I. 20×50 12nm (120Å) pore 20×100 porous silica ODS Batch C ■Peaks １.D.6×150.Shim-pack 3 Shimadzu HPLC Columns Shim-pack VP-ODS for GLP/GMP compliance For development or validation of analytical method. it would 228-34937-95 228-34937-96 228-34937-97 2.D. No. products that passed the quality criteria are delivered to be efficient to run the test with a set of three columns with packings of different batches.D. ＊An analytical column set consists of three columns whose packings (Silica-based) are from different production batches.0×150. No. Shim-pack VP-ODS has been developed to meet such expectations. 2.×length (mm) To minimize column-to-column performance deviation of ODS columns. ■Short Columns for Analytical/Preparative Cat. exchangeable cartridge 2pcs.×length (mm) Remarks 4. silica-bases. 228-34938-92 A holder for I.0mm Batch C A company's column Batch B Batch A ＊Guard column consists of a cartridge and holder. I. Holders can be used repeatedly. 4. 228-34938-94 A holder for I.6×50 5μm diameter.6×150 4. No.D. 1 Comparison of lot-to-lot repeatability (Silica-based material) 15 .6×10 Incl.D.6mm 228-34938-93 2. 〈VP-ODS Series〉 Excellent manufacturing uniformity ■Analytical Column Shim-pack VP-ODS Cat. surface treatment and packing 228-34937-91 228-34937-92 4.0×5 Incl.0×150 customers. a set of 3pcs. 228-36849-91 228-36849-92 228-36849-93 I. Shim-pack VP-ODS ■Guard Column Shim-pack VP-ODS Cat.6×250 procedures are strictly controlled respectively and only the 228-34937-93 228-34937-94 6.×length (mm) 228-34938-91 4. N-acetylprocainamide ２. exchangeable cartridge 2pcs.0×250 4. manufacturing uniformity of columns is increasing. For development or validation of method. a set of 3pcs.0×150 2. Phenol Batch B Batch A Fig. 2. Column A 1920 1060 1590 2560 Shim-pack VP-ODS 0 500 1000 1500 2000 2500 3000 NTP per 1MPa Column size: 4.25mL/g Specific surface area 410m2/g Trace metal content 30ppm max Percentage of ODS 20%C Carbon content ODS functional group Mono-functional End-capping Used 〈Number of theoretical plates and column pressure〉 The higher NTP is and the lower the pressure is. 2 Fig. Shim-pack VP-ODS shows superior performance shown Column B by NTP per 1MPa.6×150mm. spherical silica particles with a high purity Particle size 5μm Pore size 12nm Pore volume 1. propranolol ２. Mobile phase: methanol/water＝70:30. Sample: naphthalene 〈Excellent peak shapes〉 ■Peak １. Excellent peak shapes of basic compounds are achieved by thorough effective end-capping of the packings. 3 Excellent peak shape of coordination compounds is achieved by the packing base material with less metal impurities. acenaphthene ３. the easier to handle the Column C column is.0mL/min. oxine Shim-pack VP-ODS 16 Other column ■Peaks １. Flow rate: 1. .〈Packing characteristics of Shim-pack VP-ODS〉 Silica particles Entirely porous. amitriptyline Other column Shim-pack VP-ODS Fig. Shim-pack Column Performance Report Phyical Properties of Shim-pack VP-ODS Chromatographic Performance of Shim-pack VP-ODS Shim-pack Column Performance Report 〈Application Data〉 ■Peaks １.Shim-pack 〈Certificate of Analysis (Quality certificate)〉 Shim-pack VP-ODS is shipped with three types of certificate of Analysis to support method validation for HPLC analysis. 1. Benzoic acid 1 ■Peak １. 4 Analysis of Aspartame in Soft Drink Fig. Aspartame ３. Caffeine ２. 5 Analysis of Hesperidine in Orange Juice 17 . Certificate of Analysis for Packing Material (Physical Properties) 2. Certificate of Analysis for Packing Material (Chromatographic Performance) 3. Hesperidin 1 2 3 Fig. Methacrolein Butyraldehyde Benzaldehyde Veleraldehyde m-tolualdehyde Hexanaldehyde 13 7 Fig. Propionaldehyde ６. Riboflavin phosphate ３. Inosine ４. Caffeine ５. ADP ６. ９. Acetone ４. 6 5 3 6 Analysis of Nucleic Acid in Tuna Meet Fig. 11. 11 Analysis of DHPH-Aldehydes. 10 18 Analysis of Oxine Standard Fig. ATP 2 ■Peaks １. Thiamine 4 1 3 2 1 4 Fig. Formaldehyde ２. 8 Analysis of Chlorexidine in Ointment Fig. 13. Oxine Analysis of Berberine ■Peaks １. Ketones group Standard . 12. 9 ■Peak １. Nicotinamide ２. Hypoxanthine ２. Acrolein ５. Acetaldehyde ３. Phridoxine ４. 10. Berberine 1 1 Fig. Crotonaldehyde ７. IMP ３. 7 5 Analysis of Vitamin B Group in Soft Drink ■Peak １. 2-butanone 1 2 1 3 4 11 5 8 9 6 10 12 ８. Chlorhexidine ■Peak １.■Peaks １. AMP ５. For HPLC methods.8 1. To meet such requirements. Shimadzu has developed the High Throughput (HT) column packed with very fine non-porous. High-throughput HPLC The combination of the Shim-pack HT-ODS and the Shimadzu LC-2010 is sure to help you to achieve additional high throughput in your research and analyses. 3 Gradient analysis cycle: 3.000 0. 19 . it is possible to drastically reduce the gradient analysis run times while obtaining the same peak resolution. With this column. quality certificates for the uniformity of the packing material are provided for each column so it's easy to comply with validation requirements.Shim-pack Shim-pack HT-ODS High-speed HPLC columns Best Partner For High Throughput Analysis Recently.100 1: methyl paraben 6 2: ethyl paraben 3: n-poropyl parben 4: n-butyl paraben 0.2 min Validation compliance We certify the reliability of the analysis data with a 3 column set made from different lots and attach quality certificates for the uniformity of the packing material. high purity silica particles. it is essential to save analysis time and to obtain reliable results.050 5: biphenyl 6: chrysene 0. The highest performance with Shimadzu LC-2010.5mL/min 2 With 2µm and non-porous packing material.5min 4 Peaks : 0. High speed analysis 0. We are sure this column makes your analyses faster and more reliable.) Moreover.4 0. the demand for high throughput analysis has increased. Shim-pack HT-ODS provides the capability of ultra high speed and high resolution analysis.150 5 1 Flow rate: 1.0 0. it is possible to realize ultra high speed analyses (Analysis times within 1 minute. It is possible to finish an analysis within 1 minute. 6×30 2μm non-porous silica ODS A kit of columns with packings from three different production lots Remarks Shim-pack FC-ODS High throughput and high resolution column packed with 3μm porous high purity silica.6×75 For shorter analysis time 221-40511-93 Shim-pack FC-ODS 4. Description I. Description I.×length (mm) 228-37681-91 Shim-pack HT-ODS 4.High resolution by Non-porous silica Average resolution The right chart shows the time and the resolution in gradient analyses by a column packed with porous silica and one packed with non-porous silica.6×30 Remarks 221-40511-92 Shim-pack FC-ODS 4.High speed and reliable Analysis Porous silica 7. Non-porous 3 min gradient min Comparison of retention time between non-porous and porous columns.6×150 For high separation For fast analysis . Non-porous column realizes high speed and reliable analysis. ■Shim-pack HT-ODS Cat. The time to obtain the same resolution with a non-porous column is less than the time required when using a porous silica column.×length (mm) 221-40511-91 Shim-pack FC-ODS 4. Applicable to wide application range from high thoughput precisely separation analysis of complex matrix samples.5 min gradient The right chromatograms show gradient analyses with the same resolution by non-porous packed column and porous packed column.D. ■Shim-pack FC-ODS 20 Cat. No. No.D.6×30 2μm non-porous silica ODS 228-37681-92 Shim-pack HT-ODS (3 column kit) 4. Porous 15 Non-Porous 10 5 0 0 5 10 15 20 Time of gradient (min) Time of gradient and resolution Benefit of non-porous silica column . Non-porous silica column can save your precious time and provide you more satisfactory performance. Non-porous silica shows higher resolution. 6mm i. ● Adsorption even of basic compounds is completely eliminated by the Shimadzu's original secondary silylation method (except the -SIL series which is packed with silica particles without any surface treatment. so that the operational parameters for analytical runs may be easily copied for preparative work.×25cm 6.0mm i.×25cm 21 .6mm i.d. MRC.d.×25cm PRC Series Preparation (15μm) 20mm i.d.×25cm 30mm i. PRC ● High quality and outstanding performance ensured by stringent quality control.×25cm Scale-up MRC Series Operational conditions for preparation (15μm) 6.d. Analysis (5μm) HRC Series 4. ● The same silica gel solid support is used in all the columns of this series.d.Shim-pack Shim-pack HRC.d.×15cm Scale-up PRC(H) Series High separation preparation (5μm) 20mm i.×15cm 4.d.d. ● The same packing material is used in the analytical and preparative columns.×25cm 50mm i.0mm i. 0mm i.×15cm 228-24377-91 4. No.0mm i.×25cm 228-23460-92 6.×1cm 228-24389-91 Shim-pack GHRC-C8 22 Stationary phase .d. (μm) Dimensions Cat. ion exchange 4.d.×15cm 228-23460-93 4.d.6mm i.d.×25cm 228-23463-92 6.d.d.×1cm 228-24386-91 Shim-pack GHRC-TMS Trimethyl group 5 4.×25cm 228-24378-92 6. Normal phase 4. 4.×15cm 228-23463-91 4.×1cm 228-24387-91 Shim-pack GHRC-NH2 Aminopropyl group 5 4.0mm i. (μm) Silica Octadecyl group Octyl group Trimethyl group Aminopropyl group Cyanopropyl group Separation mode 5 Absorption 5 Reversed phase 5 Reversed phase 5 5 5 Reversed phase Dimensions Cat.0mm i. Silica 5 4.d.d.×1cm 228-24388-91 Shim-pack GHRC-CN Cyanopropyl group 5 4.d.6mm i.×15cm 228-24379-93 ■Guard Column for HRC Column name Shim-pack GHRC-SIL Shim-pack GHRC-ODS Particle dia.0mm i.d.×15cm 228-23463-93 4.0mm i.6mm i.d.×15cm 228-24377-93 4.0mm i.d.×25cm 228-24377-92 6.6mm i.d.×25cm 228-24379-92 6.×15cm 228-24378-91 Reversed.d.0mm i.6mm i.6mm i.d.6mm i. normal.×15cm 228-23460-91 4.6mm i.6mm i.6mm i.d.0mm i.0mm i.■Shim-pack HRC Column name Shim-pack HRC-SIL Shim-pack HRC-ODS Shim-pack HRC-C8 Shim-pack HRC-TMS Shim-pack HRC-NH2 Shim-pack HRC-CN Stationary phase Particle dia.0mm i.d.×15cm 228-24376-91 4.6mm i.×15cm 228-24378-93 4.0mm i.d.×15cm 228-24379-91 Reversed phase.×25cm 228-24376-92 6.d.×15cm 228-24376-93 4.6mm i.×1cm 228-23465-91 Octyl group 5 4.d.d.d.d. No.d.×1cm 228-23462-91 Octadecyl group 5 4. d.d.d.d.d.0mm i.×25cm 228-24383-92 Shim-pack MRC-NH2 Aminopropyl group 15 Shim-pack MRC-CN Cyanopropyl group 15 Reversed phase.×25cm 228-24383-93 20mm i. No.d. (μm) Dimensions Cat.×25cm 228-24382-93 20mm i.d.5cm 228-24386-92 Shim-pack GPRC-TMS Trimethyl group 5 8mm i. normal.×1. (μm) Separation mode Shim-pack PRC-SIL Shim-pack PRC-SIL(K) Shim-pack PRC-SIL(L) 15 Silica Shim-pack PRC-SIL(H) Shim-pack PRC-ODS(L) Octadecyl group Cat.d.d. No.×25cm 228-24381-92 Trimethyl group 15 Reversed phase 6.5cm 228-23462-93 Shim-pack GPRC-SIL(L) Silica 15 50mm i. ion. ion exchange 5 15 Cyanopropyl group Reversed phase.d.5cm 228-23465-93 Shim-pack GPRC-ODS(L) Octadecyl group 15 50mm i.×25cm 228-24384-92 23 .5cm 228-24389-92 Silica 15 30mm i.×25cm 228-23461-94 50mm i.×25cm 228-24384-93 20mm i.d.d. Silica 15 Adsorption 6.×25cm 228-23464-93 15 30mm i.d.5cm 228-24388-92 Shim-pack GPRC-CN Cyanopropyl group 5 8mm i.×1.×1. normal phase 6.×7.×25cm 228-24382-91 20mm i.0mm i.d.×1. normal.d.×1.×25cm 228-23464-91 Shim-pack PRC-C8 15 20mm i.×5cm 228-23462-94 Shim-pack GPRC-ODS(K) Octadecyl group 15 30mm i.5cm 228-24387-92 Shim-pack GPRC-NH2 Aminopropyl group 5 8mm i.×1.×25cm 228-23461-91 20mm i. No.d.d.d.×7.×25cm 228-24383-91 20mm i. (μm) Separation mode Dimensions Cat.×25cm 228-24384-91 ■Guard Column for PRC Column name Shim-pack GPRC-SIL Shim-pack GPRC-ODS Stationary phase Particle dia.×25cm 228-23461-92 Octadecyl group 15 Reversed phase 6.d.d.d. exchange 6.0mm i.×25cm 228-23464-95 Adsorption Shim-pack PRC-ODS Shim-pack PRC-ODS(K) Dimensions 20mm i.d.×25cm 228-23461-95 5 20mm i.0mm i. 228-23461-93 30mm i.×25cm 228-24381-91 20mm i.d.d.d.d.×25cm 228-24381-93 Shim-pack PRC-C8(H) Shim-pack PRC-TMS Shim-pack PRC-TMS(H) Shim-pack PRC-NH2 Shim-pack PRC-NH2(H) Shim-pack PRC-CN Shim-pack PRC-CN(H) Octyl group Reversed phase 5 15 Trimethyl group Reversed phase 5 15 Aminopropyl group Reversed.0mm i.×25cm 228-23464-94 50mm i.d.d.d.×25cm 228-23464-92 Octyl group 15 Reversed phase 6. normal phase 5 20mm i.×5cm 228-23465-94 Shim-pack GPRC-C8 Shim-pack GPRC-SIL(K) ■Shim-pack MRC Column name Shim-pack MRC-SIL Shim-pack MRC-ODS Shim-pack MRC-C8 Shim-pack MRC-TMS Stationary phase Particle dia.d. Silica 5 8mm i.d.×25cm Reversed phase Shim-pack PRC-ODS(H) 5 20mm i.Shim-pack ■Shim-pack PRC Column name Stationary phase Particle dia.d.5cm 228-23462-92 Octadecyl group 5 8mm i.0mm i.×25cm 228-24382-92 Reversed.5cm 228-23465-92 Octyl group 5 8mm i. ×25cm 228-17877-92 228-17878-91 228-17878-92 ＊Also the Shim-pack VMA which is dedicated to the analysis of HVA and VMA is available.d.6mmφ) Column name Stationary phase Particle dia.×25cm 4. (μm) 5 Separation mode Adsorption Dimensions 6.×15cm 228-00808-91 228-00809-91 228-00810-91 228-00811-91 228-00812-91 Reversed. normal phase Reversed phase 6.0mm i.×15cm 228-17872-92 228-17873-91 4. ion.d.×15cm 6.×1cm 228-18262-91 Shim-pack G-CN(4) 4.×15cm Reversed.×25cm 4.6mm i.d. normal.6mm i.0mm i.×1cm 228-18268-91 .6mm i.6mm i.6mm i.×15cm Shim-pack CLC Shim-pack CLC Series 4.×15cm 6.d. No.d.6mm i.d.×25cm 4. 6.0mm i.×1cm 228-18264-91 Shim-pack G-NH2(4) 4.×15cm 6. 228-17872-91 4.d.d.0mm i.0mm i.×1cm 228-18248-91 Shim-pack G-TMS(4) 4.d.6mm i.×15cm Shim-pack CLC(M) 4.×15cm 6. ■Shim-pack G(4) Series (Guard Column for CLC series) Column name 24 Dimensions Cat.×15cm Cat.d.Shim-pack CLC ● Low cost.d. (μm) Separation mode Shim-pack CLC-SIL(M) Silica 5 Adsorption Shim-pack CLC-ODS(M) Octadecyl group 5 Reversed phase Octyl group 5 Reversed phase Shim-pack CLC-TMS(M) Trimethyl group 5 Reversed phase Shim-pack CLC-CN(M) Cyanopropyl group 5 Reversed-phase. No.d.d.d.d.d.d.×15cm 228-17875-92 228-17876-91 4.×15cm 228-17873-92 228-17874-91 4.×25cm 4. high-performance columns for wide field of applications.×15cm 228-17876-92 228-17877-91 4.×1cm 228-18270-91 Shim-pack G-ODS 4. ■Shim-pack CLC(M) (4.d.d.×15cm Cat.×25cm ■Shim-pack CLC (6.×25cm 4. exchange 4.0mm i.d.6mm i.0mm i.d. Shim-pack G-SIL(4) 4.0mm i. 228-00807-91 Octadecyl group Octyl group Trimethyl group Cyanopropyl group Phenyl group Aminopropyl group 5 5 5 5 5 5 Reversed phase Reversed phase Reversed phase Reversed-phase.6mm i.0mm i.d.d.d.d.0mmφ) Column name Shim-pack CLC-SIL Shim-pack CLC-ODS Shim-pack CLC-C8 Shim-pack CLC-TMS Shim-pack CLC-CN Shim-pack CLC-Phenyl Shim-pack CLC-NH2 Stationary phase Silica Particle dia. ion. normal.6mm i.×15cm 228-16725-91 ＊Also the Shim-pack VMA which is dedicated to the analysis of HVA and VMA is available.d.6mm i.6mm i.d. No.0mm i.6mm i.0mm i.d. exchange 6.0mm i.0mm i.×1cm 228-18246-91 Shim-pack G-C8(4) 4.×1cm 228-18266-91 Shim-pack G-Phenyl(4) 4.d.6mm i.d.×25cm 4.6mm i.d.×15cm 228-17874-92 228-17875-91 4.0mm i.6mm i.0mm i. normal phase Phenyl group 5 Reversed phase Aminopropyl group 5 Shim-pack CLC-C8(M) Shim-pack CLC-Phenyl(M) Shim-pack CLC-NH2(M) Dimensions 4. 10.×15cm) Mobile phase: Acetonitrile/water (1/1) Flow rate: 1. Column temperature: 40℃ Detector: UV spectrophotometric detector (265nm) ■Peaks １.2mL/min. Saccharin Fig.1M phosphate buffer solution (pH 2. Column temperature: 40℃ Detector: UV spectrophotometric detector (210nm) ■Peaks １. Ethyl valerate ５. Ethyl caproate Fig. Unknown ２.5mL/min. Scopolamine ２. Ethyl acetate ２. Uracil ４. Column temperature: 50℃ Detector: UV spectrophotometric detector (260nm) Analysis of Fatty Acid Ethyl Esters ■Operational Conditions Column: Shim-pack CLC-ODS (6.0mm i. ■Peaks １. Nicotinic acid ２. Phosphatidylcholine (Lecithin) ２. Lysophosphatidylcholine (Lysolecithin) Fig.1) and 0. Thiamine ８.d.0mm i.d.×15cm) Mobile phase: [100mM phosphate buffer solution (pH 2. Atropine Uridine Thymine Adenosine Guanosine Analysis of Nucleic Acid Related Compounds ■Operational Conditions Column: Shim-pack HRC-ODS (6. ９. 17 Determination of Atropine and Scopolamine ■Operational Conditions Column: Shim-pack CLC-ODS (6. 12 ■Peaks １. Pantothenic acid ４.6) /acetonitrile (5/1) Flow rate: 1. Adenine ６.0mm i.0mm i.×15cm) Mobile phase: Acetonitrile/methanol/water (3/1/1) Flow rate: 1.5mL/min.×15cm) Mobile phase: 10mM phosphate buffer solution (pH 2. 13 Analysis of Vitamin B ■Operational Conditions Column: Shim-pack CLC-SIL (6. Riboflavin phosphate ５. Sorbic acid ２. Cytidine Fig. Sphingomyelin ３. 16 ７. Column temperature: 40℃ Detector: Refractive index detector Fig.1) and 1. Column temperature: 45℃ Detector: UV spectrophotometric detector (205nm) ■Operational Conditions Column: Shim-pack CLC-ODS (6.5mL/min.5mL/min.d.Shim-pack Shim-pack HRC.2mL/min. Pyridoxine ６.×15cm) Mobile phase: 0. CLC ■Peaks １. ８.0mm i.d.2M sodium perchlorate Flow rate: 1. 14 Determination of Sorbic Acid and Saccharin ■Operational Conditions Column: Shim-pack CLC-ODS (6. Ethyl propionate ３. Cytosine ３. Guaninc ５.d. Caffeine ７. Nicotinamide ３. Riboflavin Analysis of Phospholipids Fig. Ethyl butyrate ４.×15cm) Precolumn: Shim-pack GRD-ODS 10mM phosphoric acid buffer solution/acetonitrile (15/1) Mobile phase: Flow rate: 1.0mm i.2mM sodium octane sulfonate] /acetonitrile (9/1) Flow rate: 1.d. 15 ■Peaks １. Column temperature: 40℃ Detector: UV spectrophotometric detector (210nm) 25 . Vitamin E acetate ５. 18 Analysis of Eyewash Fig.d.1% sodium sulfonic octanate added) Flow rate: 1. Ethenzamide ６. 19 ■Operational Conditions Column: Shim-pack HRC-ODS (6. Vitamin A palmitate Fig.5mL/min. Vitamin E ３. Vitamin D2 ２. Column temperature: Ambient Detector: UV spectrophotometric detector (254nm) Determination of NAD and NADH ■Operational Conditions Column: Shim-pack CLC-ODS (6.×15cm) Mobile phase: 10mM phosphate buffer solution (pH 2. Ergosterol ■Peaks １. Dibutyl phthalate ３. Diethyl phthalate ４.0mm i.6) Flow rate: 1. Vitamin K 2 ４.5mL/min.Shim-pack HRC.d. Column temperature: Ambient Detector: UV spectrophotometric detector (254nm) ■Peaks １.0mm i.05) with 0.d. 20 Analysis of Oil Soluble Vitamines ■Operational Conditions Column: Shim-pack HRC-ODS (4.d.5mL/min. CLC ■Peaks １.d. Dioctyl phthalate ２. Column temperature: 55℃ Detector: UV spectrophotometric detector (254nm) ■Peaks １. Bromovalerylurea Fig. Column temperature: 40℃ Detector: UV spectrophotometric detector (260nm) Fig.6mm i. Dimethyl phthalate ■Operational Conditions Column: Shim-pack HRC-SIL (4. NADH ２.5mL/min. NAD ■Peaks １.0mL/min. Column temperature: Ambient Detector: UV spectrophotometric detector (254nm) .0mL/min. Detector: UV spectrophotometric detector (210nm) ■Peaks １. Salicylamide ４. Aceumlnophen ２.d.×25cm) Mobile phase: n-Hexane/ethanol (98/2) Flow rate: 1. Naphazoline chloride ３. Vitamin A acetate ２.6mm i.(4.6mm i. Chrorpheniramine maleate Fig. 23 Analysis of Vitamine D2 and Ergosterol ■Operational Conditions Column: Shim-pack HRC-SIL (4.×25cm) Mobile phase: n-Hexane/ethanol (98/2) Flow rate: 1.5) /acetonitrile (4/1) Flow rate: 1.×15cm) Mobile phase: 10mM Potassium dihydrogen phosphate /acetonitrile/phosphoric and (70/30/0.×15cm) Mobile phase: Methanol Flow rate: 1. 21 Fig. 22 Analysis of Phthalate Esters 26 Analysis of Analgesics/Antipyretics ■Operational Conditions Column: Shim-pack HRC-ODS.×15cm) Mobile phase: 10mM Ammonium dihydrogen phosphate (pH 2. Caffeine ３.6mm i. Acetylsalicylic acid ５. Neostigmine sulfate ２. Sucrose ６. Column temperature: 40℃ Detector: UV spectrophotometric detector (297nm) ■Peaks １. Hydrocortisone acetate ２.0mL/min. Column temperature: Ambient Detector: Refractive index detector Fig. Maltose ７.0mm i.×15cm) Mobile phase: n-Hexane/isopropanol (25/1) Flow rate: 1. Glucose ５.5mL/min Detector: UV spectrophotometric detector (245nm) 27 . 27 Determination of Ascorbic Acid and Erythorbic Acid ■Operational Conditions Column: Shim-pack CLC-NH2 (6. Ascorbic acid ■Peaks １.×15cm) Mobile phase: Hexane/methanol (4/1) Flow rate: 1. γ-Tocopherol ４. Dexamethasone Fig.5mL/min.d.0mm i. Lactose Fig. Column temperature: Ambient Detector: UV spectrophotometric detector (254nm) ■Operational Conditions Column: Shim-pack CNC-NH2 (6. 24 Analysis of Steroid Hormones Fig. β-Tocopherol ３.d.d.5mL/min.d. α-Tocopherol ２.0mm i.0mm i.Shim-pack ■Peaks １.×15cm) Mobile phase: Acetonitrile/10mM phosphate buffer solution (pH 2.6) (3/1) Column temperature: 40℃ Flow rate: 1. Erythorbic acid ２. Xylose ３. 25 Analysis of Tocopherols ■Operational Conditions Column: Shim-pack CLC-CN (6. δ-Tocopherol ■Peaks １. 26 Analysis of Sugars ■Operational Conditions Column: Shim-pack CLC-NH2 (6. Glycerol ２. Fructose ４.×15cm) Mobile phase: Acetonitrile/water (7/3) Flow rate: 1. 228-17882-91 228-17883-91 228-17884-91 228-17885-91 228-17886-91 Dimensions 4. (μm) Separation mode Shim-pack PREP-SIL(H)・Kit Silica 5 Adsorption Octadecyl group 5 Reversed Shim-pack PREP-ODS(H)・Kit 28 20mm i.×25cm 228-17887-91 228-17888-91 .6mm i. No. ion exchange Dimensions 20mm i. (Except the PREP-SIL which is packed with silica particles without any surface treatment.000 (More than 5.×25cm Cat.×25cm Cat.6mm i. (Columns i.) ● The residual silanol groups are end-capped by the unique silylation method. respectively.×25cm 20mm i.d.000 50mm i. PREP(K). having an inner diameter of 20mm. reversed.d.Shim-pack PREP ● Shim-pack ● The PREP Series are economical. (μm) 5 5 5 5 5 Separation mode Reversed Reversed Normal.d. and 50mm. and PREP(L) columns are universal LC columns.d.d. 15μm Shim-pack PREP(K) More than 5.000 (Both analytical and preparative) 15μm Shim-pack PREP More than 5.d.d. ● The PREP. columns are packed with fully porous spherical silica particles on which respective stationary phases are chemically bonded.) (Number of theoritical plates) (Particle dia. Shim-pack PREP Series ■Shim-pack PREP(H) (5μm) (20mmφ) Column name Shim-pack PREP-C8(H) Shim-pack PREP-TMS(H) Shim-pack PREP-CN(H) Shim-pack PREP-Phenyl(H) Shim-pack PREP-NH2(H) Stationary phase Octyl group Trimethyl group Cyanopropyl group Phenyl group Aminopropyl group Particle dia.000 (More than 4. reversed Reversed Normal.d.000 20mm i.×25cm 20mm i. 15μm Shim-pack PREP(L) More than 5.×25cm 20mm i.) 5μm Shim-pack PREP(H) More than 14.) of the same lot.d. high-performance columns for preparative LC. 30mm.d. (Except the PREP-SIL) ● The PREP(H) series columns are packed with 5μm diameter particles (same as those used in the CLC series columns) to permit preparative LC with high resolution.d.×25cm 20mm i.d. No.×25cm 20mm i. ● The PREP(H) Kit is a set of an analytical and a preparative columns which are packed with packing material (5μm dia.d.000 in NH2) 5μm Shim-pack PREP(H)·Kit More than 15.×25cm 4.000 in SIL and NH2) 30mm i. ■Shim-pack PREP(H)･Kit (5μm) (20mmφ) Column name Stationary phase Particle dia. ×7.×1.×1. 228-18274-91 228-18320-91 ■Shim-pack PREP(K) (15μm) (30mmφ) Column name Shim-pack PREP-SIL(K) Shim-pack PREP-ODS(K) ■Shim-pack PREP(L) (15μm) (50mmφ) Column name Shim-pack PREP-SIL(L) Shim-pack PREP-ODS(L) Stationary phase Silica Octadecyl group ■Shim-pack G(8).×25cm 20mm i.d.d.5cm 50mm i. 8. 228-00814-91 228-00815-91 228-00816-91 Trimethyl group Cyanopropyl group Phenyl group Aminopropyl group 15 15 15 15 Reversed Normal.0mm i.d. reversed Reversed Normal. (μm) 15 15 15 Separation mode Adsorption Reversed Reversed Dimensions 20mm i.d. (μm) 15 Separation mode Adsorption Dimensions 30mm i. ion.d.d. reversed.d. 228-18273-91 Octadecyl group 15 Reversed 30mm i.d.5cm 8.d.d.d. exchange 20mm i.×25cm Cat.d. and GL Guard columns Column name Shim-pack G-SIL(8) Shim-pack G-ODS(8) Shim-pack G-C8(8) Shim-pack G-TMS(8) Shim-pack G-CN(8) Shim-pack G-Phenyl(8) Shim-pack G-NH2(8) Shim-pack GK-SIL Shim-pack GK-ODS Shim-pack GL-SIL Shim-pack GL-ODS Use Guard column for Shim-pack PREP and PREP(H) Guard column for Shim-pack PREP(K) Guard column for Shim-pack PREP(L) Dimensions Cat.×25cm 20mm i.Shim-pack ■Shim-pack PREP (15μm) (20mmφ) Column name Shim-pack PREP-SIL Shim-pack PREP-ODS Shim-pack PREP-C8 Shim-pack PREP-TMS Shim-pack PREP-CN Shim-pack PREP-Phenyl Shim-pack PREP-NH2 Stationary phase Silica Octadecyl group Octyl group Particle dia. No.×5cm 228-18270-92 228-18246-92 228-18248-92 228-18262-92 228-18266-92 228-18264-92 228-18268-92 228-18338-91 228-18321-91 228-18339-91 228-18322-91 29 .d.5cm 8.×1. GK.5cm 8.×25cm Cat.×25cm Cat.0mm i.d. No.d.d.d.0mm i. (μm) 15 15 Separation mode Adsorption Reversed Dimensions 50mm i.×25cm 20mm i.d.5cm 30mm i.×25cm 20mm i.0mm i.d.×1. No.d. No.0mm i.5cm 8.×1.×1.×25cm 20mm i.×1.5cm 30mm i.d.5cm 8.×25cm 50mm i.×25cm 228-00817-91 228-00818-91 228-00819-91 228-17879-91 Stationary phase Silica Particle dia.×7.0mm i.×5cm 50mm i.×25cm 228-18319-91 Particle dia.0mm i.5cm 8.d. Figure 28 shows a comparison data.d. SBC series. 5μm Universal use Shim-pack FLC 4. the linear velocity of mobile phase can be increased without much lowering the column efficiency. 10μm Mobile phase consumption is less than 1/20 compared with the Shim-pack CLC series.5mm ida. 28 Comparison Data of Shim-pack FLC and CLC Comparison of Shim-pack CLC. 29. SBC. which are quite useful for process control. A smaller diameter column can provide a higher sensitivity.0mm i.Use of a small-diameter column. 5μm Mobile phase consumption is less than 1/3 compared with the Shim-pack CLC series.d. requires use of small-capacity sample injector and flow-thru cell. higher sensitivity and lower mobile phase consumption than the Shim-pack CLC series. MBC ● The Shim-pack FLC series. as shown in Fig. Fig. Dedicated HPLC systems are required.d.5mm i.d.6mm i. 3μm Fast analysis Shim-pack SBC 2. and MBC series ensure higher speeds..4. 29 30 Column Diameter and Peak Height .) must be used in a dedicated HPLC system.d.Shim-pack FLC.6mm i.) Shim-pack CLC 6mm i.d. for example. Shim-pack MBC 1.d.SBC. Comparison of Shim-pack CLC and FLC The Shim-pack FLC series columns use 3μm-diameter packing materials. (Column i. while the Shim-pack MBC (1.) is applicable to ordinary HPLC systems. and MBC These columns have different inner diameters.0mm i. This feature provide very fast analyses. however. otherwise high sensitivity will not be provided. The Shim-pack SBC (2.) (Particle dia. Fig. Applicable to ordinary HPLC systems. d.×15cm 2.0mL/min. A pre-heater connected between the sample injector and the column will solve this problem as demonstrated in Fig.×5cm 4.×5cm) Mobile phase: Acetonitrile/water (8/2) Flow rate: 2. Rebaudioside A ■Peaks １.0mm i.×15cm 2.2) Flow rate: 2.d. 228-17268-91 228-17269-91 228-17270-91 Dimensions 1. 30 Merit of Pre-heater ■Shim-pack FLC for fast LC Column name Shim-pack FLC-ODS Stationary phase Octadecyl group Particle dia. Stevioside ３.6mm i.5mm i. Fig.d.0mm i.d. reversed Octyl group 10 Reversed Shim-pack MBC-C8 1.d. (μm) Separation mode Shim-pack MBC-ODS Octadecyl group 10 Reversed Shim-pack MBC-SIL Silica 10 Adsorption Shim-pack MBC-ACN Aminopropyl group 10 Normal.5mL/min.×5cm 228-13375-92 228-13694-91 228-13695-91 228-13696-91 Particle dia. 32 Analysis of Sweet Components in Stevia Leaf ■Operational Conditions Column: Shim-pack FLC-NH2 (4. δ-Tocopherol Fig.d. 228-12812-02 1.d.d. (μm) 5 5 5 Separation mode Reversed Reversed Adsorption Dimensions 2. Rebaudioside C ４. β-Tocopherol ３.d.d.Shim-pack Advantage of Pre-heater In very fast LC using a Shim-pack FLC series column.d.6mm i.d.0mm i. Column temperature: Ambient Detector: UV spectrophotometric detector (295nm) Fig.×25cm 228-12813-02 1.d. 228-13375-91 Shim-pack FLC-SIL Shim-pack FLC-CN Shim-pack FLC-C8 Shim-pack FLC-NH2 Silica Cyanopropyl group Octyl group Aminopropyl group 3 3 3 3 Adsorption Normal. Column temperature: Ambient Detector: UV spectrophotometric detector (210nm) 31 .×5cm Cat. No. α-Tocopherol ２. ion exchange 4.×5cm) Mobile phase: n-Hexane/dioxane/ethanol (98/2/0.d.×25cm 1.×25cm Cat.×50cm 1.×15cm Cat.6mm i.6mm i.0mm i.0mm i.×5cm 4.×50cm 228-12812-05 228-12811-05 ■Shim-pack SBC small-bore columns Column name Shim-pack SBC-ODS Shim-pack SBC-C8 Shim-pack SBC-SIL Stationary phase Octadecyl group Octyl group Silica ■Shim-pack MBC micro-bore columns Column name Stationary phase Particle dia.30.0mm i. γ-Tocopherol ４.×50cm 228-12813-05 228-12814-02 228-12814-05 *Shim-pack MBC requires dedicated HPL system. reversed reversed Normal.×5cm 4. 31 Analysis of Tocopherols ■Operational Conditions Column: Shim-pack FLC-SIL (4.×50cm 1.×100cm 228-12811-10 1. No.d.0mm i.d.6mm i. Shim-pack FLC ■Peaks １ Dulcoside A ２. reversed.6mm i.5mm i.5mm i. (μm) 3 Separation mode Reversed Dimensions 4.0mm i.d. a high mobile phase flow rate can result in broadened peaks due to a temperature gradient within the column. No.d.6mm i. 5mm i. Phenacetin ４. 34 ■Operational Conditions Column: Shim-pack SBC-ODS (2.0mm i. Column temperature: Ambient Detector: UV spectrophotometric detector (260nm) Shim-pack SBC ■Peaks １.×50cm) Mobile phase: Hexane/dioxane/ethanol(98/2/0. 36 Analysis of Tocopherols ■Operational Conditions Column: Shim-pack MBC-SIL (1. Thymidine ５.×15cm) Mobile phase: Heptane/ethanol/methanol (90/80/2) Flow rate: 0.0mm i.×15cm) 50mM potassium dihydrogenphosphate methanol (93/7) Mobile phase: Flow rate: 0. Ethenzamide ■Peaks １. 35 Analysis of Estrogens ■Operational Conditions Column: Shim-pack SBC-SIL (2.d. n-Butylbenzoate Fig.d.) 32 Fig.6mL/min. Guanosine ４. δ-Tocopherol ５. Caffeine ２. γ-Tocopherol ４.5mL/min. Column temperature: Ambient Detector: UV spectrophotometric detector (250nm) Shim-pack MBC ■Peaks １. β-Tocopherol ３. Methylbenzoate ２. Estradiol ３. Estrone ２. 33 Analysis of Benzoic Acid Esters Fig. α-Tocopherol ２. Ethylbenzoate ３. Undine ３. Tocol Fig.4mL/min. Cytidine ２. Column temperature: Ambient Detector: UV spectrophotometric detector (295nm) (Micro flow-thru cell was used.Shim-pack SBC ■Peaks １.2) Flow rate: 50μL/min. Column temperature: Ambient Detector: UV spectrophotometric detector (210nm) (Micro flow-thru cell was used.) .d.×15cm) Mobile phase: Methanol/water (7/3) Flow rate: 0. Column temperature: Ambient Detector: UV spectrophotometric detector (250nm) Analysis of Nucleosides ■Operational Conditions Column: Shim-pack SBC-C8 (2.5mm i.×25cm) 10mM phosphoric acid buffer solution/acetonitrile (3/1) Mobile phase: Flow rate: 100μL/min.d. n-Propylbenzoate ４. Bromovalerylurea ３. Adenosine ■Peaks １.5mm i. 37 Analysis of Febrifuge/Analgesics ■Operational Conditions Column: Shim-pack MBC-ODS (1.d. Estriol Fig. ● The Shim-pack ISA/ISC series columns. 8mm i.d. and proteins. ● Polymer types are classified into polyacrylate type having hydrophilic gel and polystyrene type having hydrophobic gel. which use polystyrene gel as the solid support. silica gel type columns ensure higher number of theoretical plates than the polymer type. Therefore it is possible to use each depending on objecting samples. because they use 5μm or 3μm diameter packing materials in contrast to 10μm diameter packing materials used in the latter. and guanidiono compounds (ISC-05). amino acids (ISC-07). PA-QA used for analysis of proteins and nucleic acids Cation exchange Shim-pack PA-CM. ● The Shim-pack WAX/WCX series columns are suitable for analysis of nucleotides. 33 . ● The Shim-pack PA series columns use hydrophilic polymers as the solid support. ● The polymer type columns ensure better pH stability than the silica gel type and so they are applicable to wider pH range mobile phases. They are especially suitable for the preparative LC of biological substances such as proteins and nucleic acids. for preparative work. for determining the preparation conditions and 20mm i.Shim-pack Shim-pack Ion Exchange Columns ● The Shim-pack ion exchange columns are available in various types as shown below.d. The columns are available in two diameters. These columns are suitable for the analysis of sugars (ISA). utilize electrostatic reaction and hydrophobic reaction. PA-SP used for analysis of proteins and nucleic acids Anion exchange Shim-pack ISA used for analysis of sugars Cation exchange Shim-pack ISC used for analysis of amino acids and guanidino compounds Polyacrylate Polymer type (Hydrophobic gel) Polystyrene ● Shim-pack ● The ion exchange column series are classified into silica gel type and polymer type. Shim-pack WAX-1 used for analysis of nucleotides and oligonucleotides Anion exchange Silica gel type Shim-pack ion exchange columns Shim-pack WAX-2 used for analysis of proteins Chemically-bonded hydrophilic silica gel (Hydrophilic gel) Cation exchange Shim-pack WCX-1 used for analysis of proteins Anion exchange Shim-pack PA-DEAE. oligonucleotides. ×1cm 228-20763-91 Shim-pack PAG-CM Carboxy methyl group 10 8.d.) ■Shim-pack ISC.×10cm 228-20761-92 Dimensions Cat.0mm i.0mm i.d. (μm) Diethyl amino ethyl group 10 8.0mm i.×1cm 228-20764-91 Shim-pack PAG-SP Surforpropyl group 10 8.d.×10cm 228-20758-91 20mm i.d.×5cm 228-16225-91 Shim-pack WAX-2 Tertiary amino group 5 4.×5cm 228-00823-91 ISC-07 guard column (Na type) 4.0mm i.0mm i.0mm i. Shim-pack ISC-30/S0504 (For trapping Na type ammonia) 4.×5cm 228-00821-91 Dimensions Cat.×1cm 228-20762-91 Shim-pack PAG-QA Tetramethyl ammonium group 10 8.×5cm 228-00802-91 ISC-07 guard column (Li type) 4. 46. (μm) Dimensions Cat.0mm i.0mm i.0mm i.×5cm 228-16365-91 Shim-pack WCX-1 Corboxyl group 5 4.d.×10cm 228-18337-92 Shim-pack ISC-05/S0504 Na type sulfone group 5 4. No.d.0mm i. ＊It is necessary to use one of the columns. No. Column name 34 ISA guard column 4.0mm i.d. Shim-pack WAX-1 Tertiary amino group 3 4.×5cm 228-14206-91 Shim-pack ISC-30/S0504Li (For trapping Li type ammonia) 4. ■Shim-pack PA: Polyacrylate Type Ion Exchange Column Column name Shim-pack PA-DEAE Shim-pack PA-QA Shim-pack PA-CM Shim-pack PA-SP Stationary phase Particle dia. (μm) Diethyl amino ethyl group 10 Tetramethyl ammonium group 10 Carboxy methyl group 10 Surforpropyl group 10 Dimensions Cat. No.×10cm 228-20760-91 20mm i.×15cm 228-00796-91 Quaternery ammonium group 7 4.0mm i.d.d. WCX: Silica Gel Type Ion Exchange Column Column name Stationary phase Particle dia.×15cm 228-09328-91 Shim-pack Amino-Na Shim-pack ISC-07/S1504Li Shim-pack ISC-07/S2504 Li type sulfone group 7 4.d.d.d.×10cm 228-20760-92 8.d. No.×10cm 228-20761-91 20mm i.d.0mm i. No.d.d.0mm i.×25cm 228-09699-91 ■Ammonia Trap Column Column name Dimensions Cat.0mm i.8cm 228-09700-91 Shim-pack ISC-07/S1504 Na type sulfone group 7 4.×10cm 228-18837-91 Shim-pack Amino-Li Li type sulfone group 5 6.0mm i.d.×10cm 228-20759-91 20mm i. 8.×10cm 228-20759-92 8. No.×5cm 228-00797-91 . ■Guard Column ＊The following are dedicated guard columns.×1cm 228-20765-91 ＊The Shim-pack PAG columns are also used for analysis.×3.d.d. 228-16367-91) between the liquid pump and the sample injector.d.d.×10cm 228-20758-92 8. ■Shim-pack PAG: Guard Column for Shim-pack PA Column name Shim-pack PAG-DEAE Stationary phase Particle dia.×5cm 228-16366-91 ＊It is necessary to connect a precolumn (Cat. Na type sulfone group 5 6.0mm i.0mm i. ISA: Polystyrene Type Ion Exchange Column Column name Stationary phase Particle dia. in analysis of amino acids.0mm i. (See Fig. No.d.6mm i.d.d.0mm i.0mm i.■Shim-pack WAX.d.d. (μm) Dimensions Cat. gradient elution Flow rate: 1. ADP ８. Column temperature: Ambient Detector: UV spectrophotometric detector Fig. 38 Analysis of Protein Standard ■Operational Conditions Column: Shim-pack WCX-1 (4. 39 Fig.0)/sodium sulfate. ATP 12.d.×5cm) Mobile phase: 20mM phosphate buffer solution (pH 6.0mm i.d.0mL/min.85). UMP ２.0mL/min. Column temperature: Ambient Detector: UV spectrophotometric detector (280nm) Shim-pack WAX ■Peaks １. Myoglobin ３. 42 Analysis of Mononucleotides ■Operational Conditions Column: Shim-pack WAX-1 (4. GTP Poly (dT) Fig. gradient elution Flow rate: 1. gradient elution Flow rate: 1.0mm i.5)/sodium sulfate.×5cm) Mobile phase: 20mM phosphate buffer solution (pH 7)/480mM phosphoric acid buffer solution (pH 6. gradient elution Flow rate: 1. CMP ３.d.d. Hemoglobin A ３.0mm i. CTP 11.d. Column temperature: Ambient Detector: UV spectrophotometric detector (415nm) Determination of Urease ■Operational Conditions Column: Shim-pack WCX-1 (4. Hemoglobin F ２. Hemoglobin C ■Peaks １.×5cm) Mobile phase: 20mM phosphate buffer solution (pH 6. 40 Urease Analysis of Hemoglobins ■Operational Conditions Column: Shim-pack WCX-1 (4.0mL/min. Column temperature: Ambient Detector: UV spectrophotometric detector (260nm) Fig. gradient elution Flow rate: 1.0mL/min. Hemoglobin S ４. GMP ５.Shim-pack Shim-pack WCX ■Peaks １.0mm i. α-Chymotrypsinogen A ４. CDP ７.×5cm) Mobile phase: Phosphate buffer solution (pH 6. Ovalbumin ２.0mm i. AMP ４.8)/acetonitrile. Column temperature: 45℃ Detector: UV spectrophotometric detector (260nm) 35 . UDP ６. Ribonuclease A ５. 41 Analysis of Synthesized DNAs ■Operational Conditions Column: Shim-pack WAX-1 (4. GDP 10.0mL/min.×5cm) Mobile phase: 20mM phosphate buffer solution (pH 6.0)/sodium sulfate. Lysozyme Fig. UTP ９. ×5cm) Mobile phase: Tris-acetic acid buffer solution (pH 8) /sodium sulfate. α-Chymotrypsinogen A ４.6) /sodium chloride. gradient elution Flow rate: 1.d. Cytochrome C ２. Lysozyme Fig. gradient elution Flow rate: 1. Ribonuclease A ３.0mm i. Hemoglobin A ３.×1cm) Mobile phase: Phosphate buffer solution (pH 6. Ovalbumin ３.0mL/min. Myoglobin ２.■Peaks １. β-Lactoglobulin A Fig.d.0mm i. Column temperature: Ambient Detector: UV spectrophotometric detector (280nm) Fig. Column temperature: Ambient Detector: UV spectrophotometric detector (280nm) . Hemoglobin F ■Peaks １.0mL/min. α-Chymotrypsinogen A ４.×1cm) Mobile phase: Phosphate buffer solution (pH 6. Column temperature: Ambient Detector: UV spectrophotometric detector (280nm) 36 Analysis of Protein Standard ■Operational Conditions Column: Shim-pack PAG-CM (8.6) /sodium chloride.0mm i.×1cm) Mobile phase: Phosphate buffer solution (pH 6. Ribonuclease A ３.0mm i. α-Chymotrypsinogen A ４.0) /sodium chloride. Column temperature: Ambient Detector: UV-VIS spectrophotometric detector (415nm) Shim-pack PA ■Peaks １.0mL/min. 47 Analysis of Protein Standard ■Operational Conditions Column: Shim-pack PA-SP (8. 45 Analysis of Protein Standard ■Operational Conditions Column: Shim-pack PA-DEAE (8. Lysozyme ■Peaks １. gradient elution Flow rate: 1.0mL/min. gradient elution Flow rate: 1. Bovine scrum albumin ５. 43 Analysis of Protein Standard ■Operational Conditions Column: Shim-pack WAX-2 (4.0mm i.d.×1cm) Mobile phase: Tris-hydrochloric acid buffer solution (pH 8.0mL/min.0mL/min. Hemoglobin S ２. α-Lactalbumin ４.d. Myoglobin ２. 46 ■Peaks １. Myoglobin ２.×5cm) Mobile phase: Tris-sulfuric acid buffer solution (pH 7. Trypsin inhibitor Fig. Column temperature: Ambient Detector: UV spectrophotometric detector (280nm) Fig.5)/sodium sulfate. Column temperature: Ambient Detector: UV spectrophotometric detector (280nm) Fig. gradient elution Flow rate: 1.d. 44 Analysis of Hemoglobins ■Operational Conditions Column: Shim-pack WAX-2 (4. 48 Analysis of Protein Standard ■Operational Conditions Column: Shim-pack PA-CM (8. β-Lactoglobulin B ６. Carbonic anhydrase ２. Lysozyme ■Peaks １.d. gradient elution Flow rate: 1.6) /sodium chloride. Ribonuclease A ３. Conalbumin ３.0mm i. Shim-pack Shim-pack ISA. Asn ９.×15cm) Mobile phase: Lithium citrate buffer solution.0mm i. 15. 21. 430nm) Detector: Method: Post-column derivatization with arginine Met Ile Leu Tyr Phe His Lys Arg Analysis of Amino Acid Standard ■Operational Conditions Column: Shim-pack ISC-07/S1504 (4.0mm i. Gln 11. 33. gradient elution Flow rate: 0. 31. Ser ４. Glu 10. 450nm) Detector: Method: Post-column derivatization with ortho-phthalaldehyde ■Peaks １. Thy ３.6mL/min. 11. 32. Lactose ５. p-Et・NH2 ４. Sucrose ２. Ala 16.d. 17. 29.3mL/min. α-AnBA 18. 27. Tau ３. Cellobiose ３. 30. 12. 348nm. Column temperature: 38℃∼58℃ Detector: Spectrofluorophotometric detector (EX. Column temperature: 65℃ Spectrofluorophotometric detector (EX. Fructose ９. 20.4mL/min. 50 Analysis of Saccharide Standard ■Operational Conditions Column: Shim-pack ISA-07/S2504 (4. Galactose 10. Em. Maltose ４. Gly ７.0mm i. 36. 348nm. Ser ８.d. 34. 28. Val Fig. Cit 17. Cystine Ile Leu Tyr Pre β-Ala β-AiBA γ-ABA His 3-Me-His 1-Me-His Car Ans HyLys Orn Lys NH3+Et・NH2 Arg Analysis of Biological Amino Acids ■Operational Conditions Column: Shim-pack ISC-07/S1504Li (4. 25. 35. 51 10. 22. 49 ■Peaks １. Mannose ８. 23.×25cm) Mobile phase: Potassium borate buffer solution.d. Pro ６. 24. Pro 14. p-Ser ２. Asp ５. Met Fig. 26. Giucose Fig. Column temperature: 55℃ Spectrofluorophotometric detector (EX. 37.×15cm) Mobile phase: Sodium citrate buffer solution. Gly 15. Rhamnose ６. Em. α-AAA 13. Ala ８. 16. 348nm. Thy ７. gradient elution Flow rate: 0. 450nm) Post-column derivatization with ortho-phthalaldehyde Method: 37 . Sar 12. Val 19. 13. Ribose ７. Hypro ６. Em. Glu ５. ISC ■Peaks １. Xylose 11. 14. gradient elution Flow rate: 0. Cystine ９. Asp ２. 0mm i.d.0mm i.6mm i. Shim-pack GPC-801C 1.6mm i.d.×1cm 228-20812-91 ■Shim-pack GPC (dedicated to determination of chloroform) Column name Exclusion limit (polystyrene) Dimensions Cat.×30cm 228-20805-91 Shim-pack GPC-803 7×104 8.0mm i.d.0mm i.0mm i. ● The technique of GPC does not utilize such chemical reactions as partition.d.5×10 8. Therefore.×30cm 228-20809-91 Shim-pack GPC-80M 4×107. but a physical reaction. Shim-pack GPC-800P 4.×30cm 228-20806-92 Shim-pack GPC-804C 4×105 8.d. adsorption.d.d.0mm i.×30cm 228-20807-91 Shim-pack GPC-805 4×10 6 8. No.d.0mm i.0mm i.d. No.d.0mm i.×30cm 228-20805-92 Shim-pack GPC-803C 7×10 4 8.0mm i.×30cm 228-20810-91 Shim-pack GPC-807 2×108 8.0mm i.d. They ensure separation efficiency comparable to analytical columns as well as large preparative capacity.×1cm 228-20812-92 . No. Mixed gel 8. this method is suitable for the measurement of molecular weight distribution of high polymers and oligomers. separation based on molecular size of the sample components.×30cm 228-20808-91 Shim-pack GPC-806 4×107 8. ■Shim-pack GPC (dedicated to determination of tetrahydrofuran) Column name Exclusion limit (polystyrene) Dimensions Cat. that is .Shim-pack GPC ● Shim-pack GPC Series columns are used for the determination of analysis of organic solvent solution of tetrahydrofuran (800 Series).0mm i.×30cm 228-20804-92 Shim-pack GPC-8025C 2×104 8.×30cm 228-20803-91 Shim-pack GPC-802 5×103 8. ● Shim-pack GPC series columns are packed with polystyrene polymers of respective degree of cross-linking. No.d.×30cm 228-20809-92 Shim-pack GPC-80MC 4×107.×30cm 228-20804-91 Shim-pack GPC-8025 2×104 8.×30cm 228-20811-92 8 ■Guard Column (for the above) 38 Column name Dimensions Cat. ● GPC-80M (80MC. Shim-pack GPC-800CP 4.d. and ion exchange. determination of chloroform (800C Series). Mixed gel 8.d.×30cm 228-20810-92 Shim-pack GPC-807C 2×10 8.×30cm 228-20811-91 3 ■Guard Column (for the above) Column name Dimensions Cat.×30cm 228-20808-92 Shim-pack GPC-806C 4×107 8. 80MD) are mixed gel columns which get the straight of calibration curve in wide range of molecular weight.0mm i.0mm i.d.0mm i. and determination of dimethylformamide (800D series).d. so that you can choose a column that exactly meets your requirements.0mm i. ranging from analysis of high polymers to that of oligomers.d.d.5×103 8.d.0mm i.0mm i. Shim-pack GPC-801 1.×30cm 228-20803-92 Shim-pack GPC-802C 5×103 8. ● Shim-pack GPC-2000 Series are dedicated to preparative LC of tetrahydrofuran and chloroform.d.×30cm 228-20807-92 Shim-pack GPC-805C 4×106 8.×30cm 228-20806-91 Shim-pack GPC-804 4×105 8. 0mm i.Shim-pack ■Shim-pack GPC (dedicated to determination of dimethylformamide) Column name Exclusion limit (polystyrene) Dimensions Cat. Shim-pack GPC-2001* Column name 1. No.120K ２.d. Polystyrene 7.0mm i. ■Guard Column (for the above) Column name Dimensions Cat. Column temperature: Ambient Detector: UV spectrophotometric detector (254nm) Fig.35K ８. Column temperature: Ambient Detector: UV spectrophotometric detector (254nm) 39 .0mm i. 9. Shim-pack GPC-801D 1.d.d. Mixed gel 8.0mm i.d.d.d.d. 52 Calibration Curves ■Operational Conditions Mobile phase: Tetrahydrofuran Flow rate: 1.×30cm 228-23343-92 Shim-pack GPC-20025C** 2×10 4 20mm i. Shim-pack GPC-2000P (For preparative LC of tetrahydrofuran.d.0mL/min. Polystyrene M.6K ７. Polystyrene 1.d.×30cm 228-20806-93 Shim-pack GPC-804D 5 4×10 8. **For preparative LC of chloroform.100K ３.w.×30cm 228-23342-91 Shim-pack GPC-2002* 5×103 20mm i. Polystyrene 1. Polystyrene 33K ６.×30cm 228-20805-93 Shim-pack GPC-803D 7×104 8. Polystyrene 115K ５.×30cm 228-23343-93 Shim-pack GPC-2003C** 7×104 20mm i.6mm i.×30cm 228-23342-93 Shim-pack GPC-2003* 7×104 20mm i.5×103 20mm i.×30cm 228-20808-93 Shim-pack GPC-806D 4×107 8.d. 53 Analysis of Polystyrene Standard ■Operational Conditions Column: Shim-pack GPC-80M (2 columns in series) Mobile phase: Tetrahydrofuran Flow rate: 1.0mm i.d.×30cm 228-20804-93 Shim-pack GPC-8025D 2×104 8.×1cm 228-20812-93 ■Shim-pack GPC-2000 Series Exclusion limit (polystyrene) Dimensions Cat.5×103 20mm i.0mL/min.5×103 8.d.) 8mm×5cm 228-20812-95 Shim-pack GPC ■Peaks １.d.0mm i.0mm i. Shim-pack GPC-800DP 4. No.0mm i.×30cm 228-23342-92 Shim-pack GPC-20025* 2×104 20mm i.d.×30cm 228-20811-93 ■Guard Column (for the above) Column name Dimensions Cat.×30cm 228-23343-91 Shim-pack GPC-2002C** 5×103 20mm i. No.×30cm 228-20810-93 Shim-pack GPC-807D 2×108 8. n-Propylbenzene Fig.d.×30cm 228-20809-93 Shim-pack GPC-80MD 4×107.d.×30cm 228-20807-93 Shim-pack GPC-805D 4×106 8. No.0mm i.×30cm 228-23342-94 Shim-pack GPC-2001C** 1. Polystyrene 410K ４.) 8mm×5cm 228-20812-94 Shim-pack GPC-2000CP (For preparative LC of chloroform.d.×30cm 228-23343-94 *For preparative LC of tetrahydrofuran.d.×30cm 228-20803-93 Shim-pack GPC-802D 5×103 8. Column temperature: 55℃ Detector: UV spectrophotometric detector (254nm) Fig.d. Column temperature: Ambient Detector: UV spectrophotometric detector (254nm) Fig. Benzene Fig. 57 Analysis of Polythioethersulfone ■Operational Conditions Column: Shim-pack GPC-80MC (8mm i. 54 Analysis of Polystyrene Standard ■Operational Conditions Column: Shim-pack GPC-802 Mobile phase: Tetrahydrofuran Flow rate: 1. 58 Analysis of Vinyleden Poly fluoride ■Operational Conditions Column: Shim-pack GPC-80MD (8mm i.×300mm) Mobile phase: DMF (including 10mM LiCL) Flow rate: 1.0mL/min. Column temperature: 40℃ Detector: Refractive index detector .d.) and Shim-pack GPC8025 (4 pcs.×300mm) Mobile phase: Chloroform Flow rate: 0.8mL/min.×300mm) Mobile phase: Chloroform/Trichlorotrifluoroethane (8/2) Flow rate: 1.0mL/min.■Peaks １.5mL/min. Column temperature: 40℃ Detector: UV spectrophotometric detector (270nm) Fig.0mL/min.d.800 ２.0mL/min. Column temperature: 40℃ Detector: Refractive index detector 40 Fig. 55 Analysis of Epoxy Resin ■Operational Conditions Column: Shim-pack GPC-803 (4 pcs.) (8mm i.) (8mm i. Column temperature: 40℃ Detector: Refractive index detector Fig.) Mobile phase: Tetrahydrofuran Flow rate: 1. Polystyrene Mw 300 ４.×300mm) Mobile phase: DMF (including 10mM LiCL) Flow rate: 0. n-Propylbenzene ５. Polystyrene Mw 42. Polystyrene Mw 2. 56 Analysis of Polymethylbenzene ■Operational Conditions Column: Shim-pack GPC-80MC (2pcs. 59 Analysis of Polyetherimide ■Operational Conditions Column: Shim-pack GPC-80MD (2 pcs.d.800 ３. Shim-pack Shim-pack Columns for Gel Filtration / Ion Exclusion Chromatography ● Gel filtration chromatography (GFC) is used to separate water-soluble high polymers such as polysaccharides, proteins, and nucleic acids, according to the molecular sizes. ● Shimadzu HPLC columns for gel filtration chromatography and ion exclusion chromatography may be classified as follows. Silica type Shim-pack Diol Proteins and water-soluble high polymers Shim-pack SCR Saccharides(Separation is made by gel filtration and ligand exchange) Gel filtration Chromatography Columns Sulfonated polystyrene (Except SCR-101H/102H) Polymer type Polyvinyl alcohol Asahipak GS, GFA Protein, peptides, nucleic acids, saccharides Shim-pack Ion Exclusion Chromatography Columns Polymer type Sulfonated polystyrene SCR-101H Organic acids SCR-102H ● Analysis of polysaccharides and oligosaccharides・・・・Asahipak GS series are recommended. ● Analysis of monosaccharides・・・・・・・・・・・・・Shim-pack SCR series is recommended. Since the separation is made by a combination of gel filtration and ligand exchange, the selectivity differs with the type of cation. ● Analysis of proteins ・・・・・・・・・・・・・・・・・Shim-pack Diol series, and Asahipak GS series are recommended. The silica type provides sharper peaks while the polymer type ensures better alkali resistancy. ● Analysis of organic acids ・・・・・・・・・・・・・・Ion exclusion chromatography using Shim-pack SCR-101H and SCR-102H is recommended. An acidic aqueous solution (e.g. aqueous solution of perchloric acid) is used as mobile phase and the H type sulfonated styrene polymer as stationary phase. 41 ■Shim-pack SCR Column name Stationary phase Particle dia. (μm) Dimensions Cat. No. Shim-pack SCR-101N Na type sulfone group 10 7.9mm i.d.×30cm 228-07730-92 Shim-pack SCR-101C Ca type sulfone group 10 7.9mm i.d.×30cm 228-17889-91 Shim-pack SCR-101P Pb type sulfone group 10 7.9mm i.d.×30cm 228-17890-91 Shim-pack SCR-101H H type sulfone group 10 7.9mm i.d.×30cm 228-07730-93 Shim-pack SCR-102H H type sulfone group 7 8.0mm i.d.×30cm 228-17893-91 ■Guard Column for Shim-pack SCR Column name Dimensions Cat. No. Guard column SCR(N) (Dedicated to SCR-101N) 4.0mm i.d.×5cm 228-09619-92 Guard column SCR(H) (Dedicated to SCR-101H) 4.0mm i.d.×5cm 228-09619-93 Guard column SCR(C) (Dedicated to SCR-101C) 4.0mm i.d.×5cm 228-17891-91 Guard column SCR(P) (Dedicated to SCR-101P) 4.0mm i.d.×5cm 228-17892-91 Guard column SCR-102H (Dedicated to SCR-102H) 6.0mm i.d.×5cm 228-17924-91 ＊Use a guard column without fail. ■Shim-pack Diol Column name Stationary phase Particle dia. (μm) Shim-pack Diol-150 Diol group 5 Shim-pack Diol-300 Diol group 5 Dimensions Cat. No. 7.9mm i.d.×25cm 228-14775-91 7.9mm i.d.×50cm 228-14775-92 7.9mm i.d.×25cm 228-14776-91 7.9mm i.d.×50cm 228-14776-92 ＊Install a precolumn (Cat. No. 228-16367-91) between the liquid pump and the sample injector. Shim-pack ION KS ■Sample Saccharides Fig. 60 Calibration Curves for ION KS Series Columns ■Operational Conditions Column: Shim-pack ION KS (8.0mm i.d.×30cm) Mobile phase: Water Flow rate: 1.0mL/min. Column temperature: 80℃ Detector: Refractive index detector 42 Shim-pack Shim-pack SCR ■Peaks １. PEG 4000 ２. Maltose ３. Glucose ４. Fructose ５. Glycerol ６. Ethanol ７. Sorbitol ■Peaks １. Polysaccharides ２. Moltotriose ３. Maltose ４. Glucose Fig. 61 Analysis of Dextrin Fig. 62 ■Operational Conditions Column: Shim-pack SCR-101N (7.9mm i.d.×30cm) Mobile phase: Water Flow rate: 0.8mL/min. Column temperature: 60℃ Detector: Refractive index detector Analysis of Saccharide Standard ■Operational Conditions Column: Shim-pack SCR-101C (7.9mm i.d.×30cm) Mobile phase: Water Flow rate: 1.0mL/min. Column temperature: 80℃ Detector: Refractive index detector ■Peaks １. Sucrose ２. Glucose ３. Xylose ４. Galactose ５. Arabinose ６. Glycerol ７. Mannitol ８. Sorbitol Fig. 63 Analysis of Saccharide Standard ■Operational Conditions Column: Shim-pack SCR-101p (7.9mm i.d.×30cm) Mobile phase: Water Flow rate: 0.6mL/min. Column temperature: 80℃ Detector: Refractive index detector ■Peaks １. Phosphoric acid ２. α-Ketoglutaric acid ３. Citric acid ４. Pyruvic acid ５. Malic acid ６. Succinio acid ７. Lactic acid ８. Formic acid ９. Fumaric acid 10. Acetic acid 11. Levulinic acid 12. Pyroglutamic acid Fig. 64 Analysis of Organic Acids ■Operational Conditions Column: Shim-pack SCR-102H (7.9mm i.d.×30cm) (2 pcs.) Mobile phase: 5mM p-Toluene sulfonic acids aqueous solution Flow rate: 0.8mL/min. Column temperature: 40℃ Detector: Conductivity detector (pH buffer organic acids analysis system) 43 Gly-Tyr Fig. 65 Calibration Curve for Shim-pack Diol Columns ■Operational Conditions Column: Shim-pack Diol (7.0mL/min. Column temperature: Ambient Detector: UV spectrophotometric detector (280nm) 44 Fig. Ovalbumin ７.×50cm) Mobile phase: 10mM phosphate buffer solution (pH 7) and 0. Transferrin ５.0mL/min. y-Globulin ４.9mm i.9mm i.×50cm) Mobile phase: 10mM phosphate buffer solution (pH 7) and 0. Lysozyme 11. Ribonuclease A 12.9mm i.2M sodium sulfate Flow rate: 1. Enolase ４. Column temperature: Ambient Detector: UV spectrophotometric detector (280nm) ■Peaks １.×50cm) Mobile phase: 10mM phosphate buffer solution (pH 7) and 0. 67 Analysis of Human Serum ■Operational Conditions Column: Shim-pack Diol-300 (7.Shim-pack Diol ■Peaks １. Adenylate kinase ５. 69 Analysis of Polyethylene Glycol ■Operational Conditions Column: Shim-pack Diol-150 (7. Lactate dehydrogenase ３. β-Lactoglobulin ８. 68 Analysis of Albumin ■Operational Conditions Column: Shim-pack Diol-150 (7. Cytochrome C Albumin IgG Fig. PEG 1000 ４. Human serum albumin ６.1M sodium chloride Flow rate: 1.×50cm) Mobile phase: 10mM phosphate buffer solution (pH 7) and 0. 66 Analysis of Protein Standard ■Operational Conditions Column: Shim-pack Diol-300 (7. Glutamate dehydrogenase ２.×50cm) Mobile phase: Water Flow rate: 1.9mm i.2M sodium sulfate Flow rate: 1. Myoglobin 10. PEG 6000 ２. Column temperature: Ambient Detector: UV spectrophotometric detector (280nm) ■Peaks １.d. Thyroglobulin ３. Column temperature: Ambient Detector: UV spectrophotometric detector (280nm) Fig.0mL/min. Blue dextran 2000 ２.2M sodium sulfate Flow rate: 1.d.0mL/min.9mm i.d. PEG 4000 ３.d.0mL/min. Chymotrypsin ９. Column temperature: Ambient Detector: Refractive index detector .d. Ethyleneglycol Ovalbumin Lysozyme Fig. Cytochrome C 13. 0mm i.×5cm 228-17744-91 In analysis of alkali metal ions with the Shim-pack IC-C1.d. No.d. Shim-pack IC-PC1 (Dedicated to IC-C1) 8.d.×15cm 228-31076-91 Shim-pack IC-A3(S) Quaternary ammonium group 5 2.×1cm 228-17736-91 Shim-pack IC-GA3 (Dedicated to IC-A3) 4.6mm i.×1cm 228-17738-91 Shim-pack IC-GC1 PEEK (Dedicated to IC-C1 PEEK) 4.d.d.0mm i.d. Shim-pack IC-A1 Used for analysis of anions including inorganic anions and organic ions. (μm) Dimensions Cat. and alkyl amines.d.×5cm 228-17735-91 Shim-pack IC-A3 Quaternary ammonium group 5 4.6mm i.6mm i.×1cm 228-31076-92 Shim-pack IC-GC1 (Dedicated to IC-C1) 4.6mm i. acidic or basic.6mm i.×15cm 228-33366-91 Shim-pack IC-C1 Sulfone group 10 5. Also. especially when weakly acidic mobile phase is used. Shim-pack IC series columns are classified as follows.0mm i.×1cm 228-33497-92 Shim-pack IC-GC3Ⅱ (Dedicated to IC-C3) 4.×10cm 228-33497-91 Shim-pack IC-C3 Carboxylic group 7 4. Shim-pack IC-A1 Column name Quaternary ammonium group 12.d. The use of this with PIA-1000 is recommended.d.Shim-pack Shim-pack IC ● Shim-pack ● The IC series is ion exchange columns developed for ion chromatography. Shim-pack IC-A3 Provides outstanding separating efficiency in analysis of inorganic anions acid ions.×15cm 228-17737-91 Shim-pack IC-C1 PEEK Sulfone group 10 4. ■Shim-pack IC-G Column name Dimensions Cat. No.0mm i. alkali earth metal ions.0mm i. Cation Analysis Shim-pack IC series Anion Analysis ■Shim-pack IC Stationary phase Particle dia.d.×1cm 228-17734-91 Shim-pack IC-GA2 (Dedicated to IC-A2) 4.d. Shim-pack IC-GA1 (Dedicated to IC-A1) 4.×7.d. rare earth metal ions.0mm i. Shim-pack IC-A2 Low ion exchange type and possible to make the concentration of salt in mobile phase low and the background reduce more.×15cm 228-33400-91 Shim-pack IC-A2 Quaternary ammonium group 10 4.d. Compatible with mobile phases of wide pH range. No.0mm i.5 4. the use of the pre-column is recommended.d. But use only in the condition of an acid.×10cm 228-17733-91 Shim-pack IC-A1(S) Quaternary ammonium group 10 2. transition metal ions. 45 .d.6mm i. suitable for separation of ethanol amines and alkyl amines.5mm 228-41176-91 ■Precolumn Column name Dimensions Cat. Shim-pack IC-C1 Used for analysis of cations such as alkaline metal ions.d.×10cm 228-32329-91 Shim-pack IC-C3(S) Carboxylic group 7 2.6mm i.6mm i. Shim-pack IC-C3 Used for simultaneous analysis of alkaline metal ions and alkali earth metal ions.×10cm 228-33367-91 *Shim-pack IC(S) series are for semi-micro LC.6mm i. Co2＋ ６.2 mM Bis-Tris＊ Mobile phase: Flow rate: 1.5mL/min. 72 Analysis of Cations in Water (includes ammonia) ■Operational Conditions Column: Shim-pack IC-C3 (4.×15cm) Mobile phase: 0. Ni2＋ ５.d. Mn2＋ Fig.6mm i. SO42− Fig. Cu2＋ ３.(2-pyridilazo resorcinol) .d. NH4＋ ４.3M lactic acid Flow rate: 1.5mM oxalic acid Flow rate: 1. Cd2＋ ８.Shim-pack IC ■Peaks １. F− ３. Column temperature: 40℃ Detector: UV･VIS spectrophotometric detector Method: Post-column derivatization with 4.0mM p-Hydroxybenzoic acid and 3. Na＋ ２. K＋ ５. NO3− ７. PO43− ２. NO2 ５.6mm i. Rb＋ ６. 73 Analysis of Transition Metal Ions ■Operational Conditions Column: Shim-pack IC-C1 (5mm i. NH4＋ ３.0mL/min.5mL/min. CR ４. Na＋ ３.×15cm) Mobile phase: 5mM nitric acid Flow rate: 1. Column temperature: 40℃ Detector: Conductivity detector ＊Bis(2-hydroxyethyl) iminotris (hydroxymethyl) methane Analysis of Alkali Metal Ions ■Operational Conditions Column: Shim-pack IC-C1 (5. Fe3＋ ２. Zn2＋ ４. Fe2＋ ７.×10cm) Mobile phase: 2.0mm i. Mg2＋ ５. Li＋ ２. Br− ６. K＋ ４.d. Cs＋ Fig.d. 71 ■Operational Conditions Column: Shim-pack IC-A3 (4. Ca2＋ Fig. Column temperature: 40℃ Detector: Conductivity detector ■Peaks １. Column temperature: 40℃ Detector: Conductivity detector 46 ■Peaks １. 70 Analysis of Inorganic Anions ■Peaks １.0mL/min.×15cm) 8. ■Shim-pack HPC Column name Stationary phase Particle dia.×5cm 228-17792-91 Shim-pack HPC-C3 n-Propyl group 5 4. ● The ● In column packings for reversed phase LC are more hydrophobic than those for hydrophobic LC. the high-degree structures of proteins are often transmuted and the enzymes deactivated. since organic solvent is generally used as mobile phase. 47 .×5cm 228-17793-91 *It is necessary to install a pre-column between the liquid pump and the sample injector.d. ● Ethyl groups or n-propyl groups are chemically bonded as stationary phase. more important to select the stationary phase. therefore. No. In hydrophobic LC. on the other hand. ● The retention of sample components is more dependent on the degree of hydrophobicity of the stationary phase in hydrophobic LC than in reversed phase LC.d. the high-degree structures of proteins are hardly transmuted. HPC-C2 or HPC-C3.0mm i. in hydrophobic LC.Shim-pack Shim-pack HPC ● Shim-pack HPC columns have been specifically developed for the hydrophobic LC of biological high polymers such as proteins and nucleic acids. Shim-pack HPC-C2 Ethyl group 5 4. It is.0mm i. to protect the Shim-pack HPC column. but are different in the following points. Hydrophobic LC and Reversed Phase LC Both of these techniques use hydrophobic column packings. since buffer solutions containing some salt are generally used. The effect of the residual silanol groups is minimized by the unique chemical treatment technique. (μm) Dimensions Cat. 5μm in diameter and 300Å in pore diameter. ● The columns are packed with fully-porous spherical particles. reversed phase LC. Column temperature: Ambient Detector: UV spectrophotometric detector (220nm) Fig.d. α-Chymotrypsinogen A Fig. Column temperature: Ambient Detector: UV spectrophotometric detector (260nm) . Cytochrome C ２. Mobile phase: gradient elution Flow rate: 0. Mobile phase: gradient elution Flow rate: 0.d. Lysozyme ４.×5cm) Ammonium sulfate/phosphate buffer solution (pH 7). 75 Analysis of Snake Venom ■Operational Conditions Column: Shim-pack HPC-C2 (4.5mL/min.0mm i.×5cm) Ammonium sulfate/phosphate buffer solution (pH 7).5mL/min. 76 Analysis of Glucosidase ■Operational Conditions Column: Shim-pack HPC-C3 (4.5mL/min.d. 74 Analysis of Protein Standard ■Operational Conditions Column: Shim-pack HPC-C2 (4.Shim-pack HPC ■Peaks １. Column temperature: Ambient Detector: UV spectrophotometric detector (220nm) 48 Fig. Myoglobin ３. Ribonuclease A ４.×5cm) Ammonium sulfate/phosphate buffer solution (pH 7).0mm i.×5cm) Ammonium sulfate/phosphate buffer solution (pH 7).0mm i. 77 Analysis of Ribonucleic Acids ■Operational Conditions Column: Shim-pack HPC-C3 (4.d. Myoglobin ３. Mobile phase: gradient elution Flow rate: 0. Transferrin ５.0mm i. Albumin ５. α-Chymotrypsinogen A Fig. Column temperature: Ambient Detector: UV spectrophotometric detector (220nm) ■Peaks １.5mL/min. Mobile phase: gradient elution Flow rate: 0. Cytochrome C ２. No.0mm i.×1cm Cat. 228-33713-91 228-18838-91 228-17990-91 49 . 78 Shimadzu Automatic Sample Pretreatment HPLC System ■Shim-pack SPC Column name Shim-pack SPC-RP3 Shim-pack SPC-RP2 Shim-pack SPC-AE1 Stationary phase Polymer Polymer Tertiary amino group Particle dia. (μm) 9 10 10 Separation mode Reversed phase Reversed phase Anion exchange Dimensions 4. Then. In the first step. ● The Shimadzu Automatic Sample Pretreatment HPLC System is recommended. only the fraction that contains the target compounds is made to enter the analytical column.0mm i.×3cm 4. Fig.Shim-pack Shim-pack SPC ● The Shim-pack SPC has specifically developed for automatic sample pretreatment and concentration by the column switching method.d.6mm i. ● The Shim-pack SPC-AE1 column is packed with fully porous silica gel particles on which weakly basic anion exchange functions are chemically bonded.d. the switching being done with a valve. HPLC System with Automatic Sample Pretreatment Functions This system is used for analysis of samples that contain many interfering compounds. ● The Shim-pack SPC-RP3 column is packed with polymer particles and used for reversed phase LC.d.×1cm 4. the sample is roughly separated in the pretreating column. The sample pretreatment is carried out in a completely automated sequence. Analytical column 7. Precolumn 4. No. (μm) Weight Cat.Shim-pack GRD. Gel GRD-ODS Octadecyl group 30∼50 10g 228-16831-91 . Connect this between the liquid pump and the sample injector bellow. Pre-column ● Undissolved materials in mobile phase is the cause of stuffing analytical column and consuming packing materials. Pre-column (It is necessary to install a pre-column between the liquid pump and the sample injector. Oven 8. Shim-pack GRD-ODS Octadecyl group 30∼50 4. Liquid Pump 3. 1. Sample injector 6. Particurely when the concentration of the salt is high. Pre-column makes the life of analytical columns long. (μm) Dimensions Cat.0mm i. you can escape from these causes above. Installing Shim-pack GRD-ODS.d. Detector 9. Shim-pack HPC series. it is available to eliminate un-dissolved materials in salt. (In case of filter GRD) 5.×25cm 228-16557-91 Diol group 10 4.×5cm 228-16367-91 Pre-column Diol Packing materials of GRD-ODS 50 Column name Stationary phase Particle dia. ● Using GRD.d. No. WCX.0mm i. Pre-column Diol The use of pre-column is recommended when using Shim-pack Diol. Shim-pack GRD-ODS Effective particuraly when reversed phase column is used and both mobile phase pH is higher than neutral and ion pair reagent involving ritrogen such as tetrabutyl ammonium is used. Drain Fig. Mobile phase 2. Shim-pack WAX.) Column name Stationary phase Particle dia. 79 Flow line having Pre-column ■Shim-pack GRD. Protein composing amino acids Shim-pack ISC-07/S1504 Amino acid analysis Shim-pack ISC-07/S1504Li Biological amino acids Gel filtration Shim-pack ION series Gel filtration and ligand exchange Shim-pack SCR series Saccharide analysis Organic acid analysis Ion exclusion Shim-pack SCR-101H.d. cis-and trans-lsoadhumulone ４. DOMA ２.) Fig. cis-and trans-lsocohumulone ２.Shim-pack Dedicated Shim-pack Columns ● The Shim-pack Series columns may be classified as follows. Column temperature: 40℃ Fluorescence HPLC monitor (Ex. Em.×25cm) Mobile phase: Methanol/water/phosphoric acid/10% tetra ethyl ammonium (77.×15cm) Mobile phase: 3mM tartaric acid/acetonitrile (7/3) Flow rate: 1. according to the applicable type of samples. Column temperature: 50℃ Detector: UV spectrophotometric detector (270nm) Fig. HVA ■Peaks １.5/1.5mL/min.6mm i. VMA ４.0mL/min.1/3) Flow rate: 1. VLA ６.S. DOPAC ５.d. 280nm.5/22. 81 Analysis of Catecholamine Metabolites ■Operational Conditions Column: Shim-pack CLC-VMA (6. MHPG ３. 320nm) Detector: 51 . cis-and trans-lsohumulone ３.0mm i. β-Phenylchalkone (I. 80 Analysis of Hop Acids ■Operational Conditions Column: Shim-pack CLC-ODS/H (4. 102H HVA/VMA analysis Shim-pack CLC-VMA Hop acid analysis Shim-pack CLC-ODS/H Applications of the Shim-pack CLC-VMA column and the Shim-pack CLC-ODS/H column ■Peaks １. dedicated to SCR-101H 4.d.×5cm 228-17892-91 ＊The following guard column must be used.d. Shim-pack SCR-101H H type sulfone group 10 7. Shim-pack ION KS-801 1×103 8.d.d. (μm) 5 Separation mode Reversed Dimensions Cat.×5cm 228-09619-93 SCR-102H guard column.■Columns for Amino Acid Analysis Column name Stationary phase Particle dia.d. No.×10cm 228-18837-91 Shim-pack Amino-Li Li type sulfone group 5 6.0mm i.×30cm 228-17889-91 Shim-pack SCR-101P Pb type sulfone group 10 7.d.d.0mm i.d.d.9mm i.d. No.d.0mm i. Na type sulfone group 5 6. 6.d. ISC-07 guard column (Na type) 4. 4. No. (μm) Dimensions Cat.d. dedicated to SCR-101P 4.0mm i.0mm i.×30cm 228-17890-91 Column name Dimensions Cat.0mm i.d. dedicated to SCR-101N 4.0mm i.×30cm 228-07730-92 Shim-pack SCR-101C Ca type sulfone group 10 7. Shim-pack SCR-101N Na type sulfone group 10 7.×30cm 228-17893-91 Column name Dimensions Cat.d.×25cm 228-00808-92 ■Columns for Hop Acid Analysis Column name Shim-pack CLC-ODS/H 52 Stationary phase Octadecyl group .d.×15cm 228-09328-91 Shim-pack ISC-07/S1504Li Li type sulfone group 7 4. (μm) Dimensions Cat.0mm i.0mm i.×5cm 228-17924-91 ■Columns for VMA/HVA Analysis Column name Shim-pack CLC-VMA Stationary phase Polar group Particle dia.0mm i. SCR(N) guard column.0mm i.×5cm 228-09619-92 SCR(C) guard column.0mm i. No.×5cm 228-00797-91 Shim-pack Amino-Na ＊The following guard columns are available for amino acid analysis. No.×30cm 228-17897-91 ＊The following dedicated guard column is available Column name Dimensions Cat.×30cm 228-17896-91 Shim-pack ION KS-804 4×105 8.0mm i.9mm i. SCR(H) guard column. (μm) 5 Separation mode Reversed Dimensions Cat.9mm i. No.×5cm 228-17898-91 Column name Stationary phase Particle dia.×15cm 228-17255-91 Particle dia.0mm i.d.d.d. No. No.9mm i.0mm i. dedicated to SCR-101C 4.0mm i.0mm i.0mm i.6mm i.×5cm 228-14206-91 Shim-pack ISC-30/S0504Li (for trapping Li type ammonia) 4.×5cm 228-00802-91 ISC-07 guard column (Li type) 4. No. dedicated to SCR-102H 6.0mm i. (μm) Dimensions Cat. No. Shim-pack ION KS-800P (Common to ION KS series) 6.×30cm 228-17894-91 Shim-pack ION KS-802 1×104 8.d.×5cm 228-00821-91 ■Columns for Saccharide Analysis Column name Exclusion limit (dextran) Dimensions Cat.×30cm 228-17895-91 Shim-pack ION KS-803 5×104 8.d.d.d. ＊The following column must be used in amino acid analysis. Column name Dimensions Cat.d. No. ■Columns for Organic Acid Analysis Column name Stationary phase Particle dia.×10cm 228-18837-92 Shim-pack ISC-07/S1504Na Na type sulfone group 7 4.×5cm 228-17891-91 SCR(P) guard column.d. Shim-pack ISC-30/S0504Na (for trapping Na type ammonia) 4.×15cm 228-00796-91 Column name Dimensions Cat.0mm i.×30cm 228-07730-93 Shim-pack SCR-102H H type sulfone group 7 8. ■Standard Values of Maximum Sample Loads (column length is 250mm) Column internal diameter (mm) Sectional area (cm2) Flow rate (ml min−1) 04.d.17 00.Shim-pack Shim-pack Preparative Columns ● The Shim-pack preparative columns are available as follows.8 Maximum sample load (mg) 17 20. the upper limit is the maximum acceptable sample load based on this peak distortion. extreme peak distortions can occur.d.0 90.d.×30cm 228-23342-93 Shim-pack GPC-2003 7×104 20mm i.d.5×103 20mm i. the desired component is sufficiently soluble in the mobile phase. Asahipak (refer to P. It shoud be noted that these standard values are applicable on condition that various requirements are satisfied: ion suppression for the desired component is achieved.0 2000 ■Polymer type GPC Preparative Columns ■Shim-pack GPC-2000 series Preparative columns of Shim-pack GPC-800 series (Tetrahydrofuran) Exclusion limit (polystyrene) Dimensions Cat.59) and CAPCELL PAK (refer to P. No.×5cm 228-20812-95 Shim-pack GPC-2000CP 53 .×30cm 228-23343-92 Shim-pack GPC-20025C 2×104 20mm i.×30cm 228-23343-93 Shim-pack GPC-2003C 7×104 20mm i. and other components do not elute near the target peak.0mm i.21) Silica type Shim-pack PREP (Refer to P.d.0 300 50.d. The table below shows standard values of maximum sample loads for a semipreparative column and preparative column each having a length of 250mm. in comparison with an ordinary analytical column.66) preparative columns are available.70). Standard values for maximum sample loads When the sample load is increased beyond some maximum.28) Synthetic high polymer preparative columns Polymer type Shim-pack GPC (Refer to P.38) Biological high polymer preparative columns Polymer type Shim-pack PA (Refer to P.×30cm 228-23342-91 Shim-pack GPC-2002 5×103 20mm i.d.×30cm 228-23342-94 (guard column) 8.×30cm 228-23343-94 (guard column) 8.0mm i.33) Preparative columns for general use Shim-pack Preparative Columns Also STR (refer to P.×5cm 228-20812-94 Shim-pack GPC-2000P ■Shim-pack GPC-2000 series Preparative columns of Shim-pack GPC-800C series (Chloroform) Exclusion limit (polystyrene) Dimensions Cat.5×103 20mm i.d. Shim-pack PRC (Refer to P. Shim-pack GPC-2001 Column name 1.×30cm 228-23342-92 Shim-pack GPC-20025 2×104 20mm i. the type of sample injection solvent is considered.0 3.×30cm 228-23343-91 Shim-pack GPC-2002C 5×103 20mm i.6 0. No.1 15.d.0 200. Shim-pack GPC-2001C Column name 1.d. 5cm 50mm i.d.×25cm 20mm i.d.×25cm 20mm i.d.d. No.5cm 8mm i.×7.d.×10cm 228-20760-92 Shim-pack PA-SP Sulfopropyl group 10 20mm i.d.5cm 30mm i.d.5cm 8mm i.×7.×1cm 228-20763-91 Shim-pack PAG-CM Carboxymethyl group 10 8.5cm 228-24387-92 228-24388-92 Cyanopropyl group Silica Silica Octadecyl group Octadecyl group 5 15 15 15 15 8mm i. normal phase 20mm i. No.d.×25cm 20mm i.×1.d.0mm i.×25cm 30mm i.×1.×25cm 50mm i.×25cm 20mm i.d.×25cm 20mm i. Shim-pack GPRC-SIL Shim-pack GPRC-ODS Shim-pack GPRC-C8 Shim-pack GPRC-TMS Shim-pack GPRC-NH2 Shim-pack GPRC-CN Shim-pack GPRC-SIL(K) Shim-pack GPRC-SIL(L) Shim-pack GPRC-ODS(K) Shim-pack GPRC-ODS(L) Silica Octadecyl group Octyl group 5 5 5 8mm i.■Polymer Type Ion Exchange Preparative Column ■Shim-pack PA Column name Stationary phase Particle dia. Shim-pack PAG-DEAE Diethylaminoethyl group 10 8.×10cm 228-20761-92 ■Guard Column for Shim-pack PA Column name Stationary phase Particle dia.d.d.d.×1.d.×1. normal.5cm 228-23462-92 228-23465-92 228-24386-92 Trimethyl group Aminopropyl group 5 5 8mm i.d. No.5cm 50mm i.d.×25cm Reversed.×25cm 30mm i.d.d.d. (μm) Dimensions Cat.×25cm 20mm i.d. No.×5cm 228-24389-92 228-23462-93 228-23462-94 228-23465-93 228-23465-94 .×1.d.d.×1. 228-23461-93 228-23461-94 228-23461-95 228-23461-91 228-23464-93 228-23464-94 228-23464-95 228-23464-91 228-24381-93 20mm i.d.×10cm 228-20758-92 Shim-pack PA-QA Tetramethyl ammonium group 10 20mm i.d. (μm) Silica Separation mode Dimensions 20mm i. ion exchange 20mm i. Shim-pack PA-DEAE Diethylaminoethyl group 10 20mm i. (μm) Dimensions Cat.×25cm Reversed-phase. (μm) Dimensions Cat.×25cm Cat.d.0mm i.d.×10cm 228-20759-92 Shim-pack PA-CM Carboxymethyl group 10 20mm i.0mm i.×25cm 50mm i.×25cm 20mm i.×1cm 228-20762-91 Shim-pack PAG-QA Tetramethyl ammonium group 10 8.d.×25cm Reversed 20mm i.5cm 8mm i.×25cm 228-24381-91 228-24382-93 228-24382-91 228-24383-93 228-24383-91 228-24384-93 228-24384-91 15 Adsorption 5 Octadecyl group Octyl group Trimethyl group Aminopropyl group Cyanopropyl group 15 Reversed 5 15 5 15 5 15 5 15 5 Reversed ■PRC guard column 54 Column name Stationary phase Particle dia.×5cm 30mm i.d.d.d.×1cm 228-20764-91 Shim-pack PAG-SP Sulfopropyl group 10 8.d.d.×1cm 228-20765-91 ■Preparative column for general use ■Shim-pack PRC Column name Shim-pack PRC-SIL Shim-pack PRC-SIL(K) Shim-pack PRC-SIL(L) Shim-pack PRC-SIL(H) Shim-pack PRC-ODS Shim-pack PRC-ODS(K) Shim-pack PRC-ODS(L) Shim-pack PRC-ODS(H) Shim-pack PRC-C8 Shim-pack PRC-C8(H) Shim-pack PRC-TMS Shim-pack PRC-TMS(H) Shim-pack PRC-TMS Shim-pack PRC-NH2(H) Shim-pack PRC-CN Shim-pack PRC-CN(H) Stationary phase Particle dia.d.0mm i.d. Column temperature: Ambient Detector: 220nm 55 .1％(v/v)Acetate water/Acetonitriles (3/2) Flow rate: 60mL/min.×250mm L.1 to 1％(v/v) trifluoreacetic acid. 84 ■Large scale preparative conditions Sample: Moutan bark extract. cell) Detector: ■Preparative purification of Paeonol in Moutan Bark Paeonol Fig. 10mmL. Detector 210-230nm Fraction concentration Evaporator (organic solvent) → freeze-drying (water) → storage at −20℃ Fig.×25cm) Mobile phase: Acetonitriles/water (1/6) Flow rate: 0.6mm i.5mmL.1％(v/v)Acetate water/Acetonitriles (3/2) Flow rate: 0. 5μm) Mobile phase: 0. Column temperature: Ambient UV spectrophotometric detector (230nm.2mmL.5mL/min. as required) Mobile phase Acetonitrile/water (or 2-propanol). C4.d.d. in the presence of 0. 200mL. Detector: UV spectrophotometric detector (254nm. 200mL. In case of preparative purification.d.1％(v/v) sloution of trifluoroacetic acid in acetonitrile Gradient B 8 → 18％ (20 min) Flow rate: 15mL/min. 82 Fig. the point is that evaporating a fraction easily to make the mobile phase acidic using trifluoroacetic acid and acetate Preparative Scale Purification of Peptides Column Reversed phase TMS(C1).) A: 0. pumped-injection Shim-pack PREP-ODS (L) (50mm i.d. formic acid or acetic acid. ODS(C18) (size-exclusion or ion exchange column. changing mobile phase into acidic makes it possible to restrain the sticking of peptides to the surface of the silica gel.d. 83 ■Large scale preparative conditions Sample: Paeonyroot etract. pumped-injection Column: Shim-pack PREP-ODS (L) (50mm i.×25cm) Mobile phase: Acetonitriles/water (1/6) Flow rate: 100mL/min. Column temperature: 40℃ UV spectrophotometric detector (254nm) Detector: ■Preparative purification of Peptides When separating peptides in reversed phase chromatography. 15μm) Column: Mobile phase: 0. cell) Detector: ■Fraction purity test condition Sample: Fraction 5μL Column: Shim-pack CLC-ODS (M) (4. 85 ■Fraction purity test condition Sample: Fraction 10μL Column: STR ODS-H (4mm i. 0. 86 Preparative purification of synthetic Peptides Sample: Column: Mobile phase: Synthetic peptide (10 amino acids) 100mg/10mL aqueous solution Shim-pack PREP-ODS(H)kit (20mm i. C8.8mL/min. 0.1％(v/v) aqueous trifluoroacetic acid B: 0. cell) Fig. Column temperature: Ambient UV spectrophotometric detector (230nm.Shim-pack ■Preparative purification of Paeoniflorin in paeony root Paeoniflorin Fig.×25cm.×15cm. 0. 87 Separation of Esters phthalate in using GPC column Column: Shim-pack GPC 2001C (20mm i.5mL injection Column: Shim-pack GPC-20025C＋2002C＋2001C Mobile phase: Chloroform Column temperature: Ambient Detector: Refractive index detector (128×12−6RIUFS) . Figure 88 shows a flow chart for the recycling procedure. Diheptylphthalate ３. Didecylphthalate ２. In this point. recycling method might be used to improve separations when the difference of the molecular size can be noticed and peak top is separated.×30cm)×2 Mobile phase: Chloroform Flow rate: 3mL/min. However.5mmL. Dibutylphthalate Fig. In these cases.d. With reference to the separation of low molecular.■Preparative in using GPC column Various HPLC conditions must be accepted to increase the sample load. 89 Sample: 56 Recycle separation chromatogram of Styrene oligama Polystyrene molecular weight marker (molecular weight 760) 1％(W/V) chloroform solution 0. an enhanced separating effect can be obtained as if the column length were increased by re-introducing the eluate band containing the desired component eluted from the column back into the column inlet. One of these conditions. ■Peaks １. pump sample injector Preparative purification column UV absorption detector Automatic recycle valve Recycling path Out flow path Fraction collector Mobile phase Fig. no additional mobile phase is consumed at all. GPC using chloroform as mobile phase has an advantage rather than reversed phase chromatography. cell) Detector: ■Recycling in preparative purification Since preparative purification columns become more expensive as their size increases. In the recycling mode. mobile phase that has high sample solubilities should be used. 88 Flow Chart of Recycling System One Eight Fig. GPC is inferior to reversed phase chromatography. it may be inevitable to use a column of such length that high resolution is not ensured. Column temperature: Ambient UV spectrophotometric detector (260nm. Switching the recycle valve permits choice of the out flow path leading to the fraction collector and the recycling path leading to the pump inlet. ×15cm 4.6mm i.d.d. ion exchange.6mm i.125 ∼ 0. and hydrophobic.Shim-pack Shim-pack BIO(T) ● The Shim-pack BIO(T) series columns. Merits of Titanium Column 〈Corrosion Resistancy Comparison of Titanium and Stainless Steel〉 In the HPLC of biological samples.6mm i.×5cm 4.6mm i. (μm) 5 3 5 5 5 5 Pore diameter (Å) 100 100 300 300 300 300 Dimensions 4. ● The Shim-pack BIO(T) series columns are available in three types: reversed phase.×5cm 4.5 ∼ 1.5m/year × : More than 1.d. corrosive liquids such as sodium chloride solution and hydrochloric acid are often used Solvent 1 10 25 25 ◎ × ◎ ○ Sulfuric acid 1 10 25 25 ◎ ○ ◎ ○ Nitric acid 25 65 boiling boiling ◎ ○ ◎ ◎ Acetic acid 10 60 boiling boiling ◎ ○ ◎ ◎ Formic acid Sodium chloride 10 25 25 25 ○ ○ ◎ ◎ Ammonium chloride Zinc chloride 40 20 25 25 ○ ○ ◎ ◎ Ferrous chloride Sodium hypochlorite 30 5 25 25 × △ ◎ ◎ chloride solution and hydrochloric acid.d.g. The Shim-pack BIO(T) series is recommended for such cases.25mm/year ■Shim-pack BIO(T) Series Column name Shim-pack CLC-ODS(T) Shim-pack WAX-1T Shim-pack WAX-2T Shim-pack WCX-1T Shim-pack HPC-C2T Shim-pack HPC-C3T Separation mode Reversed Anion exchange Anion exchange Cation exchange Hydrophobic Hydrophobic Particle dia.×5cm 4.d.125mm/year △ : 0.d. using titanium column tubing. Pure titanium Hydrochloric acid as the mobile phase. No. e.6mm i.×5cm 4. 228-18062-91 228-18257-91 228-18258-91 228-18259-91 228-18260-91 228-18261-91 57 .25mm/year ○ : 0. than the 316 stainless steel which is generally used in HPLC systems. especially against that by halogen ions. the Shimadzu LC-7A System. have been developed for biocompatible HPLC system. Titanium is far more resistant against corrosion by sodium Concentration Temperature 316 stainless (%) (℃) steel ◎ : Less than 0.6mm i.×5cm Cat. Column temperature: Ambient Detector: UV spectrophotometric detector (280nm) IgG Fig. gradient elution Flow rate: 1.1% aqueous solution of TFA/0.×5cm) Mobile phase: 0.1% acetonitrile solution of TFA.0mL/min. Myoglobin ３.0) /sodium chloride. Trypsin inhibitor Fig. 90 Analysis of Protein Standards ■Operational Conditions Column: Shim-pack WAX-2T (4. Transferrin ４. α-Lactalbumin ５.×5cm) Mobile phase: Tris-hydrochloric acid buffer solution (pH 8.6mm i.d.d.×5cm) Mobile phase: Phosphate buffer solution (pH 6. Column temperature: Ambient Detector: UV spectrophotometric detector (215nm) . 92 Determination of IgG Monoclonal Antibody in Mouse Ascites Fluid ■Operational Conditions Column: Shim-pack WAX-2T (4.6mm i. Ovalbumin ２.6mm i. α-Chymotrypsinogen A ４.d. 93 Analysis of Decomposition Products of Trypsin in Cytoch C ■Operational Conditions Column: Shim-pack CLC-ODS(T) (4.d. gradient elution Flow rate: 1. Lysozyme ■Peaks １.Shim-pack BIO(T) ■Peaks １. 91 Analysis of Protein Standards ■Operational Conditions Column: Shim-pack WCX-1T (4. Ribonuclease A ５.0)/sodium chloride.0mL/min. Column temperature: 40℃ Detector: UV spectrophotometric detector (280nm) 58 Fig.0mL/min.0) /sodium chloride. Myoglobin ２. gradient elution Flow rate: 1. gradient elution Flow rate: 1. Column temperature: Ambient Detector: UV spectrophotometric detector (280nm) Fig. Carbonic anhydrase ３.×5cm) Mobile phase: Tris-hydrochloric acid buffer solution (pH 8.0mL/min.6mm i. d.0mm i. 4.×15cm 406-020 4E 4. ■Guard Column (Analytical) Column name Asahipak C8P-50 Stationary phase Particle dia.d.×25cm 406-018 6D C18 group Particle dia. some drugs. ● Having no silanol groups. No. No. ● The column is stable in a pH range of 2∼13.0mm i.d.d. Asahipak C4P-50G C4 group 5 4. 4.6mm i. (μm) 5 Dimensions GLC Cat.×15cm 406-017 6. No.×15cm 406-026 4.d.d. It can be washed with an alkaline solution.× 1cm 406-025 Asahipak C8P-50G ■Asahipak ® C4P Column name Asahipak C4P-50 Stationary phase C4 group Particle dia.×1cm 406-022 6. (μm) 5 Dimensions GLC Cat.6mm i.×1cm 406-028 59 . (μm) Dimensions 4. (μm) 5 6E ■Guard Column (Analytical) Column name Asahipak ODP-50G Stationary phase C18 group Particle dia.d. ● The gel has a minimum swelling and contraction.6mm i.d.×1cm 406-019 GLC Cat. e.×25cm 406-027 ■Guard Column (Analytical) Column name Stationary phase Particle dia. ■Asahipak ® ODP Column name Asahipak ODP-50 Dimensions GLC Cat. this column is especially effective for the HPLC of basic compounds.6mm i. and so the column can be used in a wide mobile phase condition.×25cm 406-024 4. from 100% water to 100% water-soluble organic solvent.6mm i.d.0mm i.g. No.×25cm 406-021 6.d.Asahipak ® Asahipak ® ODP ● The Asahipak ODP column is packed with synthetic hard polymer particles on which octadecyl groups are chemically bonded and is used for reversed phase LC.6mm i. No.d. 4D Stationary phase 4. ● High repeatability in gradient LC.6mm i.6mm i. (μm) Dimensions GLC Cat.d.6mm i.×15cm 406-023 C8 group 5 4. 6mL/min.0mL/min. Insulin Fig.d. β-Endorfin ３. β-Endorfin ２. 1-Dodecanol ７. 98 Separation of Vitamin D2 and D3 ■Operational Conditions Column: Asahipak ODP-50 (6. Insulin ３. Column temperature: 30℃ Detector: UV spectrophotometric detector (260nm) . Column temperature: 30℃ Detector: UV spectrophotometric detector (220nm) Fig. 94 Separation of Alkylalcohols Group ■Operational Conditions Column: Asahipak ODP-50.×15cm) Mobile phase: Acetonitrile/water (7/3) Flow rate: 1.6mL/min.×15cm) Mobile phase: 0. (4.×15cm) Mobile phase: Methanol 80/water 20 Flow rate: 0. Octanol ５.×15cm) Mobile phase: Acetonitrile/water (95/5) Flow rate: 1. 1-Decanol ６. Column temperature: 30℃ Detector: UV spectrophotometric detector (254nm) 60 Fig. Insulin b chain ４.3) 73/Acetonitriles 27 Flow rate: 0. 95 Separeation of Macromolecule Peptide ■Operational Conditions Column: Asahipak C8P-50. Column temperature: 30℃ Detector: UV spectrophotometric detector (220nm) ■Peaks １.6mm i. C8P-50. C4P-50 (4.d. Ethylene glycol ２. Butanol ３. Creatinine ２. 96 Separeation of Macromolecule Peptide ■Operational Conditions Column: Asahipak C4P-50.6mm i. Column temperature: 30℃ Detector: Refractive index detector ■Peaks １. Vitamin D2 ２.05％TFA (pH＝2.■Peaks １. Methyl benzoate ３.3) 72/Acetonitriles 28 Flow rate: 0.6mm i.0mm i.d. Big gastrin ■Peaks １. n-Hexyl benzoate Fig.×15cm) Mobile phase: 0. n-Propyl benzoate ４. Vitamin D3 ■Peaks １.0mL/min.05％TFA (pH＝2. Substance P ２.d.6mL/min.0mm i.d. Hexanol ４. 1-Tetradecanol Fig. 97 Analysis of Standard Samples ■Operational Conditions Column: Asahipack ODP-50 (6. (4. 1％ TFA Solvent B: Acetonitrile and 0.1％ TFA Linear gradient from solvent A to solvent B in 100minutes. Lysozyme 11.05％ TFA A/B＝Linear gradient from 90/10 to 40/60.0mm i.05% TFA/acetonitrile (4/1) Flow rate: 1.0mm i.d. Column temperature: 30℃ Detector: UV spectrophotometric detector (254nm) ■Peaks １. Column temperature: 30℃ Detector: UV spectrophotometric detector (210nm) ■Peaks １. Neurotensin ５. Leu-Enkephalin Fig. (6.×15cm) Mobile phase: Solvent A: Water and 0.0mL/min.×15cm) Mobile phase: Solvent A: Water and 0. 30minutes Flow rate: 1. Cytochrome C ３. 102 Analysis of Decomposition Products of Trypsin in Cytochrome C ■Operational Conditions Column: Asahipak ODP-50 (6. 104 Analysis of Peptide Mixture ■Operational Conditions Column: Asahipak ODP-50 (6.0mL/min. Vitamin B12 Fig. Column temperature: 30℃ Detector: UV spectrophotometric detector (280nm) Fig.0mL/min. Ovalbumin Fig. 101 Separation of Protein Standard ■Operational Conditions Column: Asahipak ODP-50 (6. 103 Analysis of Peptide Mixture ■Operational Conditions Column: Asahipak ODP-50 (6.×15cm) Mobile phase: Methanol/acetonitrile/10mM sodium phosphate (2/1/1) (pH 9) Flow rate: 1. Substance P ７.0mL/min. Bacitracin ８. α-Chymotrypsinogen A ５.0mL/min. Column temperature: 30℃ Detector: UV spectrophotometric detector (220nm) 61 . Leu-Enkephalin ６.Asahipak ® ■Peaks １.0mm i. Mastoparan 12.05% TFA/acetonitrile (4/1) Solvent B: 0. Gly-Gly-Leu ３. Cinchonine Fig.05% TFA/acetonitrile (1/1) Linear gradient from solvent A to solvent B in 20 minutes Flow rate: 1.d. Myoglobin Fig.d. Column temperature: 25℃ Detector: UV spectrophotometric detector (215nm) ■Peaks １. Vitamin B6 ２. 100 Separation of Quinine and Cinchonine ■Operational Conditions Column: Asahipak ODP-50 (6.×15cm) Mobile phase: 0. Flow rate: 1.×15cm) Mobile phase: Acetonitriles 1/50mM phosphoric acid sodium Flow rate: 1.0mm i. Met-Enkephalin ４. Met-Enkephalin ４. Insulin ９.×15cm) Mobile phase: Solvent A: 0.0mm i. Column temperature: 30℃ Detector: UV spectrophotometric detector (250nm) ■Peaks １.d.05％ TFA Solvent B: Acetonitrile and 0. 99 Separation of Vitamins B Group ■Operational Conditions Column: Asahipak ODP-50.d. Insulin B chain 10. Ribonuclease ２. Lys-Bradykinin ２.0mL/min. Quinine ２.d.0mm i. Vitamin B2 ４. Gly-Gly-Ala ２. Vitamin B1 ３. Bradykinin ３. Lysozyme ４. 000 ＞10.Asahipak ® GS ● The Asahipak GS series columns are packed with hard gel particles made of vinyl alcohol polymer.000 ＞11. 700. 220HQ. 620HQ 406-322 .000 40.000.5M. ● The allowable pH range of mobile phase is 2∼12 for the GS-320 and GS-520.6mm i.d. 620.d.×10mm 220. or SDS (sodium dodecyl sulfate) are also applicable.000 ＞16.000 406-323 406-324 9 7 1.000 300.6mm i. ■High-Resolution Type (7.000 ＞16. (It is recommended that the concentration is below 0. 710HQ 406-327 GS-2G 7B 7. 520HQ. and 2∼9 for the other columns.000 406-320 406-321 ■Guard Columns Column name 62 Dimensions Grade GLC Cat.000.000 ＞16. ● Aqueous solutions of salts and buffer solutions can be used as mobile phase. No.000 406-325 406-326 GS-220HQ GS-320HQ 6 6 3.×10mm 310. 40. ● Various organic solvents can be used. 320HQ 520.000 1. guanidine HCI.000 ＞16.000 406-318 406-319 GS-520HQ GS-620HQ 7 7 300. GF-1G 7B 7.6mm i.×30cm) Type Applicable both to aqueous solutions and organic solvent Applicable to aqueous solutions Column name Average particle diameter (μm) GF-310HQ GF-510HQ 5 5 GF-710HQ GF-7MHQ Exclusion limit (molecular weight) (Pullulan) Number of theoritical plates GLC Cat.000 2. 320.000 ＞15.d.) Mobile phases that contain urea. 510HQ 710. 510. 710HQ.000 ＞15.000. No. 310HQ. surfactants. gelatins. peptides. nucleic acids. nucleic acids 620 Highly polymerated proteins. globulin. nucleic acid constituents. oligomers. hydrophilic polymers 510 Proteins. vitamines. gelatins 710 Polysaccharides. sugars. oligomers.5M ■Applications 320 520 220 620 GS-710 ● ● ● ● ● Methanol 0∼100% ● ● Below 20% Ethanol 0∼100% ● ● Below 20% 0∼50% ● ● ● 51∼100% ● THF 0∼100% ● DMF 0∼100% ● Acetone 0∼100% ● Chloroform 0∼100% ● 0∼50% ● 51∼100% × Acetonitrile DMSO Not applicable 310 Hydrophobic polymer. hydrophilic polymers ■Calibration Curves Fig. hydrophobic/hydrophilic polymers 220 Oligosaccharides. monomers 320 Peptides. monomers. steroids. hydrophilic. 105 Fig. albumins.310. blood metabolites 520 Plasma proteins.Asahipak ® ■Applicable Organic Solvents Solvent conditions Solvent Applicability Concentra. nucleic acids. 7M tion Aqueous solution 0∼0. sugars. 510 710. sugars. 106 Columns for Aqueous Mobile Phases Columns for Aqueous/Organic Solvent Mobile Phases 63 . hydrophilic polymers. dextran. and 0.3% ethanol (pH 4. No.000 406-109 406-108 The use of preparative guard column GS-20G (228-18754-02) is recommended.02 0. Nonmercaptoalbumin ■Peaks １.0) Solvent B: 50mM sodium phosphate and 500mM sodium chloride (pH 7.×10cm) Mobile phase: 50mM N-methyl piperazine. 108 Determination of Mercaptoalbumine and Nonmercaptoalbumine in Control Serum ■Operational Conditions Column: Asahipak ES-502N (7.6mm i.0mL/min. 107 Analysis of Protein Standard ■Operational Conditions Column: Asahipak ES-502C (7.0) Linear gradient from solvent A to solvent B in 20 minutes Flow rate: 1. Mercaptoalbumin ４. ● The columns are available in two types.×10cm) Column name Average particle diameter (μm) Ion exchange group Ion exchange capacity (meq/g) Number of theoritical plates GLC Cat.0mL/min.d. Detector: UV spectrophotometric detector (280nm) 64 Fig.Asahipak ® ES ● The Asahipak ES series columns. Myoglobin ２.000 ＞3. hence have little hydrophobic adsorption.d. Globulin ２. ● Since the gel particles have many ion exchange groups and alcoholic hydroxyl groups. ■Analytical Column (7. ■Peaks １. ● The columns ensure high yield for proteins. anion exchange type and cation exchange type.55±0. are packed with vinyl alcohol polymer particles into which ion exchange groups have been introduced.5 9±0.02 ＞3. Column temperature: 35℃ Detector: UV spectrophotometric detector (280nm) .d. ES-502C ES-502N 9±0. Uric acid ３. ● The allowable pH range of mobile phase is 2∼12. Lysozyme Fig. 400mM sodium sulfate. enzymes. α-Chymotrypsinogen A ４.×10cm) Mobile phase: Solvent A: Sodium phosphate (pH 7. the columns are suitable for the separation of ionic biological compounds. Ribonuclease A ３.6mm i.55±0.8) Flow rate: 1. developed for high-speed ion exchange chromatography.5 Carboxyl group Diethylamino ethyl group 0. and nucleic acids.6mm i. (4. 112 Separation of Dextran hydrolysis substances ■Operational Conditions Column: Asahipak NH2P-50. (4. Maltotriose ４.0mL/min.6mm i.d. Glucose ２. Isomaltotriose ４. Maltoheptaose ■Peaks １.Asahipak ® Asahipak ® NH2P ● The Asahipak NH2P series columns are for sugar analysis. Isomaltose ３.6mm i. Isomaltooctaose ９. Sucrose ４.0mL/min. Rafinose Fig. Maltose ３. Lactose Fig. Asahipak NH2P-50 4E NH2 group 5 4. Maltopentaose ６.×25cm) Mobile phase: Acetonitriles/Water Flow rate: 1.6mm i. Isomaltoheptaose ８. Maltose ６. ● Available under the condition of the alkali (less than pH＝13) and you can wash the column easily as well as getting high separation. Fructose ２. Isomaltohexaose ７.0mL/min.6mm i. Column temperature: 30℃ Detector: Refractive index detector Fig. Ramnose ２.0mL/min.d.×25cm) Mobile phase: Acetonitriles 60/Water 40 Flow rate: 1. ● High number of theoritical plates and symmetrical peak can be aquired between monosaccharides and origosacharides. (4. Asahipak NH2P-50G 4A NH2 group 5 4. Column temperature: 30℃ Detector: Refractive index detector Fig. packed with vinylalcoholcopolymer with polyamines chemicalybonded.6mm i. (μm) Dimensions GLC Cat. ● The column is stable in a pH range of 2∼13. Disaccharides and Trisaccharides ■Operational Conditions Column: Asahipak NH2P-50.d.×25cm 406-030 ■Guard Column (Analytical) Column name Stationary phase Particle dia. (μm) Dimensions GLC Cat.×25cm) Mobile phase: Acetonitriles 75/Water 25 Flow rate: 1.×1cm 406-031 ■Peaks １. 110 ■Peaks １. 109 Separation of Monosaccharides and Disaccharides ■Operational Conditions Column: Asahipak NH2P-50.6mm i. Glucose ３. Detector: Refractive index detector 65 .×25cm) Mobile phase: Acetonitriles 75/Water 25 Flow rate: 1. Maltohexaose ７. Glucose ４.d. No.d. 111 Separation of Monosaccharides. Fructose ３. Sucrose ５. Isomaltopentaose ６. No. Glucose ２.d. Isomaltononaose ■Peaks １. Maltotetraose ５. Column temperature: 30℃ Detector: Refractive index detector Separation of Oligosaccharides ■Operational Conditions Column: Asahipak NH2P-50. Isomaltotetraose ５. (4. ■Asahipak NH2P Column name Stationary phase Particle dia. (2) CAPCELL PAK is classified as follows. AG Type Only 120Å Type pore diameter SG Type 120Å and 300Å Type pore diamete CAPCELL PAK (3) Selection of CAPCELL PAK columns SG type AG type Aliphatic carboxylic acids and esters Aromatic carboxylic acids and esters Other compounds Flavonol       Oil-soluble viamine Catechin group    Porphyrin Dansyl amino acid Steroid Aromatic hydrocarbons Catecholamine Nucleic acids base Nucleoside Peptide Saponin Amines Quaternary ammonium salt Proteins Water-solved vitamine As for the amount of micro-metal involved in silica. (μm) Column i.6 C18 120 5 6.d.CAPCELL PAK (1) Features of CAPCELL PAK As covered with silicones polymer on the surface of silica. ■CAPCELL PAK AGType (C18 Type) Stationary phase Pore diameter (Å) Particle dia. No. 4. SG type has fewer than AG type. superior in chemical stability rather than silica type materials.0 20 30 66 Column length (mm) GLC Cat. 35 12501 150 12503 250 12504 35 12506 150 50 12508 15501 250 250 15504 16501 . 0 20 Column length (mm) GLC Cat. 35 12510 150 12512 250 12513 35 13510 150 50 13512 16510 250 250 35 150 250 35 150 50 250 16513 16501 12520 12522 12523 13520 13522 16520 16523 67 . No.6 C18 120 5 6.6 C8 120 5 6. 4.CAPCELL PAK ■CAPCELL PAK AGType (C8 Type) Stationary phase Pore diameter (Å) Particle dia.d.d. No. (μm) Column i. 35 21501 150 250 21503 21504 35 150 50 250 21506 21508 25501 25503 ■CAPCELL PAK SGType (C18 Type) Stationary phase Pore diameter (Å) Particle dia. 4.0 20 Column length (mm) GLC Cat.0 20 30 4. (μm) Column i.6 C18 300 5 6. No.d. Column temperature: 40℃ Detector: UV spectrophotometric detector (235nm) Injection volume: 10μL (5μg/mL) ■CAPCELL PAK SG Type (C8 Type) Stationary phase Pore diameter (Å) Particle dia.×25cm) Mobile phase: 0. Ethylparaben ４.5)/MeOH/CH3CN＝50/35/15. 1. Column temperature: 40℃ Detector: UV spectrophotometric detector (210nm) Separation of an Antiseptic Component ■Operational Conditions Column: C18 SG120 (4.6 C8 300 5 6.d. p-Hydroxy benzoic acid ３. Lysozyme ４. Sorbic acid ７. 4mM cetyltrimethylammonium chloride Flow rate: 1mL/min. Pyridoxine hydrochloride ５. 35 21511 150 21513 250 21514 35 21516 150 21518 50 25511 250 25513 35 21521 150 21523 250 21524 35 21526 150 21528 50 25521 250 25523 ■Peaks １. 115 Separation of Protein with SG type columns ■Operational Conditions Mobile phase: (A) 0.■Peaks １.5mL/min.×25cm) Mobile phase: Water (pH2. 114 ■Operational Conditions Column: C18 SG120 (4. Nicotinamide ４.5mM heptanesulfonic acid Flow rate: 1. (μm) Column i.0 20 4.1％ TFA/H2O (B) 0. BSA ５. Salicylic acid ■Peaks １. n-Propylparaben ６. H3PO4)/CH3CN ＝9/1.1. Ribonuclease A ２.6mm i. 113 Separation of Aqueous Vitamine Group Fig. Calcium pantothenate ３. Ovalbumin Column Column Fig. Myoglobin ６.d.6mm i. Methyiparabon ２. n-Butylparaben 10. Column temperature: 40℃ Detector: UV spectrophotometric detector (214nm) 68 . Dehydroacetic acid ５.1％ TFA/CH3CN (B) 15％ → 60％ (15min) Gradient Flow rate: 1. iso-Butylparaben ９. Thiamine Fig.6 C8 120 5 6.0 20 Column length (mm) GLC Cat. Folic acid ７. Cytochrome C ３. Biotin ８. Ascorbic acid ２.5mL/min. Caffeine ６. (mm) 4. Riboflavin ９.05M NaH2PO4(pH4. Benzoic acid ８. d.×25cm 880952-710 4. No.d.d.×15cm 883952-701 4. The kit consists of an empty column.d.×25cm 883952-704 880952-704 Guard Column Pre-column Kit In analyses with a Zorbax column.×15cm 880952-706 883952-710 4.×15cm 880952-702 883952-706 4.6mm i. (μm) Separation mode Silica 5 Adsorption Octadecyi group 5 Reversed Octyl group 5 Reversed Trimethyl group 5 Reversed Zorbax SIL Zorbax ODS Zorbax C8 Zorbax TMS Zorbax NH2 Aminopropyl group 5 Reversed.6mm i.d. 116 Flow Line Having Guard Column and Pre-column 69 .6mm i.×25cm 880952-705 883952-703 880952-703 4.6mm i. (Except Zorbax SIL) ■Zorbax Column name Stationary phase Particle dia.6mm i.6mm i.d.×25cm 4.×15cm 883952-705 4.×15cm 4. normal Zorbax SAX Quaternary ammonium group 5 Anion exchange Sulfonic group 7 Cation exchange Zorbax SCX-300 Dimensions GLC Cat. columns are packed with fully-porous.6mm i.6mm i.×25cm 4. the pioneer in HPLC.×25cm 4.×15cm 4.6mm i.6mm i. 4.d.6mm i.d. it is recommended to use a guard column or a pre-column so that a longer service life is expected of the Zorbax column.d. exchange Zorbax CN Cyanopropyl group 5 Reversed.6mm i. and a filter. normal. Fig.d.d. packing material.Zorbax Zorbax ● The ● The Zorbax columns are high-performance columns developed by DuPont.×25cm 883952-708 880952-708 4.d.×15cm 880952-701 883952-702 4.6mm i.6mm i.6mm i. anion.d.d.6mm i.×15cm 4.d.×25cm 4.d. spherical silica particles on which the respective stationary phases are chemically bonded. (μm) 5 Column i. 404-031 ■STR ODS Column for Preparative HPLC Column name STR ODS-Ⅱ Pore dia. (mm) 20.6 Column length (mm) 10 10 10 10 GLC Cat. (mm) 4. 404-023 404-024 404-025 404-028 Particle dia. (Å) 120 ■Guard Column for Analytical HPLC Column name ■Guard Column for Preparative HPLC Column name STR ODS-Ⅱ 70 Pore dia.6 6. and uses high-purity (99.d.0 STR ODS-Ⅱ 120 5 4.STR ODS Series ● STR (STR : Shimadzu Techno Research) ODS Series columns using octadedecyl group as the stationary phase feature low cost and wide field of applications.d. (mm) 20. No. 17% in carbon content.6 Particle dia. (Å) 120 . (Å) Particle dia. (μm) STR ODS-Ⅱ 120 5 STR ODS-Ⅱ PEEK 120 5 Column i. (μm) 5 Column i. No. Suitable for analysis of less-polar/basic compounds. (mm) 4. 15% in carbon contents. Suitable for analysis of highly polar/acidic compounds. 404-019 250 150 250 404-018 404-021 404-020 150 150 250 404-022 404-027 404-026 5 4. No.d.0 Column length (mm) 250 GLC Cat. 404-030 Pore dia.999%) silica gel Highly resistant against acids and alkalis.0 STR ODS-Ⅱ PEEK 120 Column length (mm) 150 GLC Cat. No. ■The STR ODS-Ⅱ Columns ■STR ODS Column for Analytical HPLC Column name Pore dia.0 4. ■The STR ODS Series is available in the following three types: STR ODS-H STR ODS -M STR ODS STR ODS -Ⅱ ● ● ● ● ● ● 19% in carbon contents.0 Column length (mm) 50 GLC Cat.d.6 6. (μm) Column i.0 4. (Å) Particle dia. Formaldehyde ２.d.d. (μm) ODS-H 100 5 Column i.6 ■STR ODS-H ■Peaks １. 19. His ８. 117 Analysis of Aldehydes Fig.d. ⊿Tyr Met Val Pro Arg Trp Phe Lys Ile Leu Analysis of PHT-Amino Acids ■Operational Conditions Column: ODS-H (4. 15. 16.6 4 ODS-M 100 5 4. Glu ３.5 mL/min. (Å) Particle dia. 228-21831-01 228-21831-02 228-21831-03 228-21832-01 228-21832-02 228-21832-03 2. 228-21336-02 228-21336-01 228-21336-03 228-21336-04 228-21336-05 228-21830-01 228-21830-02 228-21830-03 228-21830-04 Column length (mm) 250 50 250 250 50 250 Cat. Gln ５. Pentanal Fig. Butylaldehyde ５. 20.8 mL/min. Asn ４. 12. Acetaldehyde ３. 14. 17. (μm) PREP ODS-H 120 5 Column i. Vitamin A ２.0 mm i. Analytical HPLC Column name Pore dia.d.0 mm i. Ala 10. 118 ■Operational Conditions Column: ODS-H (4.070% SDS)/acetonitrile (60/40) Column temperature: 40℃ Flow rate: 0. (mm) 20 4. 13.5 mL/min. Ser ６. Detector: UV spectrophotometric detector (269 nm) 71 . 18.STR ■STR ODS Columns 1.×25 cm) Mobile phase: 10mM Sodium formate buffer solution (pH 5. Preparative HPLC Column name Pore dia. Thr ７. Tyr Fig.d. Detector: UV spectrophotometric detector (260 nm) ■Peaks １.×15 cm) Mobile phase: Methanol Column temperature: 40℃ Flow rate: 0. 119 11. Asp ２.50 and containing 0. Vitamin E ■Peaks １.×15 cm) Mobile phase: Methanol/water (40/60) Column temperature: 40℃ Flow rate: 0. (mm) 4 4. Propylaldehyde ４. Em: 440nm) Analysis of Oil-Soluble Vitamines ■Operational Conditions Column: ODS-H (4.6 Column length (mm) 50 150 250 150 250 150 250 150 250 Cat. Gly ９. (Å) Particle dia.0 mm i. Vitamin D2 ３. No. No. Detector: Fluorophotometric detector (Ex: 360 nm.6 PREP ODS-M 120 5 20 4. Ribofiavin phosphate ６. Detector: UV spectrophotometric detector (210 nm) Analysis of Food Presevatives ■Operational Conditions Column: ODS-M (4. Phenobarbital ４. Benzoic acid ２. Detector: UV spectrophotometric detector (210 nm) ■STR ODS-M ■Peaks １.×25 cm) Mobile phase: Water/acetonitile (6/1) Column temperature: Ambient Flow rate: 15 mL/min. Propionic acid Fig. Primidone ３.d.3 with phosphoric acid) Column temperature: 40℃ Flow rate: 1.5)/methanol (7/3) Column temperature: 50℃ Flow rate: 0. Ribofiavin Fig. Detector: 20μL 72 Fig.d. Naphthalene ４. Malonic acid ４. Nicotinic acid ２.5mM Sodium octane sulfonate (9/1) Column temperature: 40℃ Flow rate: 1. Pyridoxine ５. Sorbic acid ■Peaks １. Thiamine ７. 123 Analysis of Organic Acids ■Operational Conditions Column: ODS-M (4.0 mL of solution) . Carbamazepin ■Peaks １.×15 cm) Mobile phase: 100mM phosphate buffer solution (pH 2. 120 Analysis of Water Soluble Vitamines ■Operational Conditions Column: ODS-Ⅱ (4. Biphenyl Fig.0 mL/min.×15 cm) Mobile phase: 25mM Phosphate buffer solution (pH 3. Uracil ２. Acetic acid ５.×15 cm) Mobile phase: 10mM KH2 PO4 (adjusted to pH 2. Caffeine ８. Citric acid ６. 122 Fig.×15 cm) Mobile phase: 100mM phosphate buffer solution (pH 2.d. Calcium pantothenate ４. 125 Preparative LC Chromatogram of Paeoniflorin in Peony Extract ■Operational Conditions Column: PREP ODS-H (20 mm i.0 mL/min.1) /methanol/acetonitrile (4/2/1) Column temperature: 40℃ Flow rate: 1.d. Malic acid ３. Methyl benzoate ３.6 mm i. Succinic acid ７. Tartaric acid ２. Ethosuximide ２.d.0 mm i.0 mL/min.1) and 1.d.0 mm i. Detector: UV spectrophotometric detector (254 nm) ■STR PREP ODS-H ■Peaks １.6 mm i. Nicotinamide ３.0 mL/min.■STR ODS-Ⅱ ■Peaks １.8 mL/min. 121 Analysis of Anticonvulsant ■Operational Conditions Column: ODS-Ⅱ (4. Phenytoin ５.×25 cm) Mobile phase: Methanol/water (85/15) Column temperature: Ambient Flow rate: 8. Detector: UV spectrophotometric detector (230 nm) Sample size: 100 mg of peony extract (2. 124 Inspection Data ■Operational Conditions Column: PREP ODS-H (20 mm i. Detector: UV spectrophotometric detector (210 nm) Fig. Fructose ２. Formic acid ５. Column temperature: Ambient Detector: Refractive index detector Determination of Organic Acids in Soy Sauce ■Operational Conditions Column: Shim-pack SCR-102H (8. Glucose ３. Lactate ３.×30 cm) Mobile phase: Water Flow rate: 1. NO3− ７. Galactose ４.×15 cm) Mobile phase: Actonitrile/water(7/3) Flow rate: 1.0 mm i.d.2mM potassium biphthalate (pH 4.×30 cm) Mobile phase: 5mM p-toluenesulfonic acid Flow rate: 0. Acetic acid ６.4 Application Data Food Industry ■Peaks １. Pyroglutamic acid Fig. 128 Determination of Saccharides in Pickles ■Operational Conditions Column: Shim-pack SCR-101C (7.d. α-Tocopherol ２. Acetate ２.d.d.0mm i.5 mL/min. SO42− Fig.8 mL/min. 131 Determination of Tocopherols in Soy Bean Oil ■Operational Conditions Column: Shim-pack CLC-NH2 (6.×15 cm) Mobile phase: n-Hexane/isopropanol (25/1) Flow rate: 1.2 mL/min. 129 Determination of Saccharides in Miso (Bean paste) ■Operational Conditions Column: Shim-pack ISA-07 (4. × 25 cm) Mobile phase: Potassium borate buffer solution. Malate ６. Column temperature: 40℃ Detector: Conductivity detector ■Peaks １. Citrate ８. 126 Analysis of Anions in Wine ■Peaks １.d.5 mL/min.×15 cm) Mobile phase: 1. γ-Tocopherol ４.6 mL/min. Lactic acid ４. 320nm. gradient elution Flow rate: 0. Succinic acid ３. Maltose ２. Em.430nm) Method: Post-column derivatization with arginine ■Peaks １.6 mm i. Cl− ５. Malic acid ２. iso-Maltose ５. Fructose ４. Sucrose ２. Sorbitol Fig. Glucose ■Peaks １.9 mm i.0 mm i. β-Tocopherol ３.2) Flow rate: 1. δ-Tocopherol ■Peaks １. Glucose ３. 127 ■Operational Conditions Column: Shim-pack IC-A1 (4. Column temperature: 85℃ Detector: Refractive index detector Fig. 130 Determination of Saccharides in Soft Drink ■Operational Conditions Column: Shim-pack CLC-NH2 (6.d. Fructose ３. Column temperature: 65℃ Detector: Fluorescence detector (Ex. Column temperature: 45℃ Detector: Conductivity detector Method: Post-column derivatization with bis-tris buffer Fig. Tartarate ９. Sucrose Fig. Column temperature: Ambient Detector: UV spectrophotometric detector (297nm) 73 . Succinate ４.0 mm i.0 mL/min. d.5 mL/min.d. Glu ５. 12. Column temperature: Ambient Detector: Fluorescence detector (Ex. Detector: Fluorescence detector (Ex.1M phosphate buffer solution (pH 2.×15 cm) Mobile phase: Sodium citrate buffer solution.1)/acetonitrile (3/1) Mobile phase: Flow rate: 1. Column temperature: 40℃ Detector: UV spectrophotometric detector (210nm) ■Peaks １. ATP Fig.■Peaks １. 14.3 mL/min.×15 cm) 0. 133 Determination of Hesperidine in Orange Juice ■Operational Conditions Column: Shim-pack CLC-ODS (6.5 mL/min. 135 ■Peaks １.5 mL/min. Rebaudioside A Fig. 348nm. 136 Determination of Theanine in Tea Leaf ■Operational Conditions Column: Shim-pack CLC-ODS (6. 15.0 mm i. Pro ６. 132 Analysis of Adenine Derivative 6 Components ■Operational Conditions Column: STR ODS-Ⅱ (4. Em. AMP ５. 16. IMP ３.×15 cm) Mobile phase: Acetonitrile/water (3/1) Flow rate: 1. 435nm) Method: Prelabelling with OPA 74 Analysis of Preservatives in Food ■Operational Conditions Column: Shim-pack CLC-ODS (6.×15 cm) Mobile phase: 100mM phosphate (triethylammonium) buffer solution (pH 6.d. 137 ９.6 mm i. Ala ８. Gly ７. Inosine ４. Sorbic acid Fig. 11. Stevioside ２. 13. Asp ２.1)/acetonitrile (92/8) Mobile phase: Flow rate: 1.0 mm i. Val Theanine Fig. gradient elution Flow rate: 0. Column temperature: 40℃ Detector: UV spectrophotometric detector (260nm) Hesperodine Fig.9) /acetonitrile (20/1) Flow rate: 1.d.5 mL/min. Hypoxanthine ２. Em. ADP ６. 365nm.×15 cm) Precolumn: Shim-pack GRD-ODS 10mM phosphate buffer solution (pH 7. Met Ile Leu Tyr Phc His Lys Arg Analysis of Amino Acids in Soy Sauce ■Operational Conditions Column: Shim-pack ISC-07 (4.0 mm i. Ser ４. 10. 134 Determination of Steviosides in Pickles ■Operational Conditions Column: Shim-pack CLC-NH2 (6.0 mm i.×15 cm) Precolumn: Shim-pack GRD-ODS Mobile phase: 10mM phosphate buffer solution (pH 6.d. Column temperature: 40℃ Detector: UV spectrophotometric detector (265nm) ■Peaks １.0 mL/min. 450nm) Method: Prelabelling with OPA . Benzoic acid ２.8) 100/acetonitrile Flow rate: 1.d. Thr ３. Column temperature: 40℃ Detector: UV spectrophotometric detector (225nm) Fig.0 mm i. d.6) and 0.1M phosphate buffer solution (pH 2. Caffeine ７. Pantothenic acid ４.) ５.9mm i.d.S. Column temperature: 50℃ Detector: UV spectrophotometric detector (220nm) Berberine Amygdalin Fig.0mm i. Nicotinic acid ２.×15cm) Mobile phase: 10mM phosphate buffer solution (pH 2.d. Thiamine ８.×15 cm) Mobile phase: 0.d. 138 Analysis of Vitamin Tablet ■Operational Conditions Column: Shim-pack CLC-ODS (6. 315nm) Benzothorium Chloride Taurine Fig. Nicotinamide ３.5 mL/min. 141 Determination of Benzethonium Chloride in Gargle ■Operational Conditions Column: Shim-pack CLC-ODS (6.1) and 1.0 mm i.0 mL/min. Riboflavine Fig. 139 Determination of Ethinylestradiol in Cream ■Operational Conditions Column: Shim-pack CLC-SIL(M) (4.Medicines and Cosmetics ■Peaks １.5 mL/min.×30cm) Mobile phase: 10mM aqueous solution of perchloric acid Flow rate: 0.5 mL/min.d.1)/acetonitrile (1/2) Mobile phase: Flow rate: 1. Ethofyline (I. Pyridoxine ６.6mm i.1M phosphate buffer solution (pH 2. 143 Determination of Amygdalin in Apricot Seed ■Operational Conditions Column: Shim-pack CLC-ODS (6. Em. Column temperature: 40℃ Detector: UV spectrophotometric detector (210nm) Ethinylestradiol Fig.×15cm) 0.0mm i.5 mL/min.2mM sodium octane sulfonate/acetonitrile (9/1) Flow rate: 1.8 mL/min.×25cm) Mobile phase: n-Hexane/ethanol (20/1) Flow rate: 1. Column temperature: 40℃ Detector: Fluorescence detector (Ex.×15cm) Mobile phase: Water/acetonitrile (4/1) Flow rate: 1.d. Column temperature: 50℃ Detector: UV spectrophotometric detector (340nm) Fig.0mm i. Column temperature: 40℃ Detector: UV spectrophotometric detector (210nm) 75 . 285nm. Column temperature: 40℃ Detector: Refractive index detector Fig. 140 Determination of Taurine in Soft Drink ■Operational Conditions Column: Shim-pack SCR-101H (7.1M sodium perchlorate/acetonitrile (2/1) Flow rate: 1. 142 Determination of Berberine in Coptis Japonica Powder ■Operational Conditions Column: Shim-pack CLC-ODS (6. Column temperature: 40℃ Detector: UV spectrophotometric detector (335nm) Chlorhexidine gluconate Chondroitin sulfate Fig. 15.0 mL/min.0mm i. Cysteine ７. 16. Camphor ２.×15cm) Mobile phase: 10mM phosphate buffer solution (pH 2. Column temperature: 40℃ Detector: Refractive index detector Fig.d. 145 Determination of Iodoform in Pulveres ■Operational Conditions Column: Shim-pack CLC-ODS (6.×25cm) Mobile phase: 50mM phosphate buffer solution (pH 3) Flow rate: 1.6) /acetonitrile (1/2) Flow rate: 1. gradient elution Flow rate: 0. Gly ８. Asp ２. Column temperature: Ambient Detector: UV spectrophotometric detector 76 Determination of Chondroitin sulfate in Eyewash ■Operational Conditions Column: Shim-pack Diol-300 (7.0mL/min. Detector: UV spectrophotometric detector (210nm) Fig.0mm i.0mm i. 14.d.6mm i. 148 Determination of Fluocinolone Acetonide in Cream ■Operational Conditions Column: Zorbax CN (4. Menthol Fig.3 mL/min.d. Met Ile Leu Try Phe His Trp Lys Arg Analysis of Amino Acid in Transfusion Liquid ■Operational Conditions Column: Shim-pack ISC-07 (4.5 mL/min. Pro ６.lodoform ■Peaks １. Methyl salicylate ３. 18. 348nm.0mm i.×25cm) Mobile phase: n-Hexane/ethanol(4/1) Flow rate: 1. 144 Analysis of Antiphlogistic/Analgesic Paste ■Operational Conditions Column: Shim-pack CLC-ODS (6.9mm i. Thymol ４.×15cm) Mobile phase: Citric acid buffer solution.×15cm) Mobile phase: Methanol/tetrahydrofuran/water (5/1/4) Flow rate: 1.1M sodium perchlorate/acetonitrile (3/2) Flow rate: 1. 11.d. Thr ３. Glu ５.6) and 0.d. Em. 450nm) Method: Post-column derivatization with OPA .×15cm) Mobile phase: 10mM phosphate buffer solution (pH 2.2 mL/min.d. 17. 12. 13. Ala ９. 147 ■Peaks １. Val Fluocinolone acetonide Fig. Column temperature: 55℃ Detector: Fluorescence detector (Ex. 149 10. Column temperature: 50℃ Detector: UV spectrophotometric detector (240nm) Fig. 146 Determination of Chlorhexidine Gluconate ■Operational Conditions Column: Shim-pack CLC-ODS (6.5 mL/min. Ser ４. 0mm i. 151 Determination of Vitamin B12 in Injection ■Operational Conditions Column: Shim-pack CLC-ODS (6. Dihydrocodeine phosphate ４. Column temperature: 40℃ Detector: UV spectrophotometric detector (210nm) 77 . Acetaminophen ２.6) /acetonitrile (7/1) Flow rate: 1. SO42− Fig. × 15cm) Mobile phase: 2.5) (3/1) Flow rate: 1.5 mL/min. NO3− ３. Cl− ２.5 mL/min.d. Column temperature: 40℃ Detector: UV spectrophotometric detector (230nm) Fig. 152 Analysis of Inorganic Anions in Injection ■Operational Conditions Column: Shim-pack IC-A1 (4. 150 Determination of β-Glycyrrhetinic Acid in Cream ■Operational Conditions Column: CAPCELL PAK C18 (5μm) (4.×15cm) Mobile phase: 10mM phosphate buffer solution (pH 2.×15cm) Mobile phase: 10mM phosphate buffer solution (pH 2. 153 Analysis of Cold Medicine ■Operational Conditions Column: Shim-pack CLC-ODS (6.d. Column temperature: 40℃ Detector: UV spectrophotometric detector (278nm) ■Peaks １.0 mL/min.6mm i.0mm i.β-Glycyrrhrtinic acid Vitamin B12 Fig.6mm i. × 25cm) Mobile phase: Acetonitrile/phosphoric acid (pH 2.6) and 20mM sodium perchloric/acetonitrile (8/1) Flow rate: 1. Column temperature: 40℃ Detector: Conductivity detector ■Peaks １.5 mL/min.5mM phthalic acid and 2.d. Methylephedrine Fig. Caffeine ３.0) Flow rate: 1.4mM (tris) (hydroxymethyl) aminomethane (pH 4.d. 1)/methanol (1/1) Mobile phase: Flow rate: 1. 155 Determination of Acetylsalicylic Acid and Salicylic Acid in Serum ■Operational Conditions Column: Shim-pack CLC-ODS (6.1)/acetonitrile (10/1) Mobile phase: Flow rate: 1. 10. Ethosuximide ２.2 mL/min. 11. UMP ２.×15cm) Mobile phase: 0. CMP ３. 157 Determination of Xanthine. CDP ■Peaks １.5 mL/min. Salicylic acid ■Peaks １. AMP ４. GMP ５.0 mL/min.0mm i.5) /methanol(10/9) Flow rate: 1. Phenytoin ５.×15cm) 0. Acetylsalicylic acid ２. Ethofyline (I.×15cm) Mobile phase: Methanol/water (5/1) Flow rate: 1.S. 159 Determination of Vitamin 25-OH-D3 in Blood ■Operational Conditions Column: Shim-pack CLC-ODS (6. Column temperature: 50℃ Detector: UV spectrophotometric detector (265nm) .d.0mm i. gradient elution Flow rate: 1.0mm i.0mm i. Hypoxanthine ３.d. Column temperature: 55℃ Detector: UV spectrophotometric detector (210nm) ■Operational Conditions Column: Shim-pack CLC-ODS (6. 12. Column temperature: 40℃ Detector: UV spectrophotometric detector (260nm) ADP UTP GDP CTP ATP GTP 25-OH-D3 Fig. Hypoxanthine. UDP ６.1M phosphate buffer solution (pH 2. Primidone ３.5 mL/min. and Uric Acid in Urine Determination of Theophylline in Blood ■Operational Conditions Column: Shim-pack CLC-ODS (6. Column temperature: 55℃ Detector: UV spectrophotometric detector (245nm) ■Peaks １. Uric acid ２.0mm i.×15cm) 0. ９.1M phosphate buffer solution (pH 5.d.×15cm) Mobile phase: 20mM phosphate buffer solution (pH 3) Flow rate: 1. ■Operational Conditions Column: Shim-pack CLC-ODS (6. Column temperature: 40℃ Detector: UV spectrophotometric detector (270nm) ■Peaks １.0mm i. Xanthine ７.S. Carbamazepin Fig.d.d.1M phosphate buffer solution (pH 2. Phenobarbital ４. Hexobarbital (I.Medical and Biochemical Field ■Peaks １. 158 Analysis of Nucleotides in Rat Liver Oil ■Operational Conditions Column: Shim-pack WAX-1 (4. Column temperature: 45℃ Detector: UV spectrophotometric detector (260nm) 78 Fig. Theophyline ２.×5cm) Mobile phase: Phosphate buffer solution (pH 7). 156 Fig. ８.) Fig.0 mL/min.d.) ６. 154 Analysis of Anticonvulsants in Serum Fig.0 mL/min. 164 14. 15. Column temperature: 50℃ Detector: Fluorescence detector (Ex. Thr ４. gradient elution Flow rate: 1.d. Pro ９. 345nm.0mm i. N1-Acetylspermine Fig.5 mL/min. 23.0mm i. Column temperature: 50℃ Detector: Fluorescence detector (Ex. 161 ■Operational Conditions Column: Shim-pack CLC-ODS (6. Ala 11. 450nm) Method: Post-column derivatization with OPA 79 . N-Acetylputrescine ２. Met Cys Ile Lou Tyr Phe β-Ala Trp His Orn Lys Arg Analysis of Amino Acids in Serum ■Operational Conditions Column: Shim-pack ISC-07 (4. 160 Determination of Polyamines in Urine Fig.×15cm) Mobile phase: Tartaric acid/acetonitrile (97/3) with a small amount of EDTA Flow rate: 1. 440nm) Method: Post-column derivatization with OPA ■Peaks １.1 mL/min. Detector: Conductivity detector Histamine Fig. 24. Em. Val Fig.d. 16. 21. Em.×15cm) Mobile phase: Lithium citrate buffer solution. Column temperature: Ambient Detector: UV spectrophotometric detector (220nm) Determination of Histamine in Plasma ■Operational Conditions Column: Shim-pack WCX-1 (4. Em. Column temperature: 38℃∼58℃ Detector: Fluorescence detector (Ex.d. Glu ７.0mm i. N1-Acetylspermidine ４. Gly 10.×5cm) Mobile phase: 40mM phosphate buffer solution (pH 6. Tau ２.0mm i. Asn ６. 25.×5cm) Ammonium sulfate/phosphate buffer solution (pH 7).5 mL/min.×15cm) Mobile phase: Sodium perchlorate and sodium hexane sulfonate /acetonitril. 348nm.0mm i. 162 Determination of β-Glucosidase Fig. Cit 12. gradient elution Flow rate: 0. Gln ８. Putrescine ３. 22.■Peaks １. 19. 18.4 mL/min. Vanillylmanderic acid (VMA) ２. Homovanillic acid (HVA) ■Peaks １.0 mL/min. Mobile phase: gradient elution Flow rate: 0. 20.9) Flow rate: 1. 17. 455nm) Method: Post-column derivatization with OPA Determination of HVA and VMA in Neonate Urine ■Operational Conditions Column: Shim-pack CLC-VMA (6. Ser ５.d. P-Et・NH2 ３. 348nm. 163 ■Operational Conditions Column: Shim-pack HPC-C3 (4. α・A・B・A 13.d. 165 Analysis of Epoxy Resin ■Operational Conditions Column: Shim-pack CLC-ODS (6. each) Mobile phase: Tetrahydrofuran Flow rate: 1.0 mL/min.d.×15cm) Mobile phase: Methanol/water (4/1) Flow rate: 1.0mm i. Column temperature: 50℃ Detector: Refractive index detector .×15cm) Mobile phase: Methanol/0.0 mL/min. gradient elution Flow rate: 1. 166 Analysis of Ester Oligomers ■Operational Conditions Column: Shim-pack CLC-ODS (6.0mm i.Chemical Industry Fig. (8.05% phosphoric acid (3/2) Flow rate: 1.0 mL/min. and 802. Column temperature: Ambient Detector: UV spectrophotometric detector (250nm) Low molecular weight High molecular weight Fig.d. 170 Analysis of Carboxylmethylcellulose ■Operational Conditions Column: Asahipak GS-710 (two columns) Mobile phase: 50mM sodium nitrate Flow rate: 1. 169 Analysis of Polyvinylacetate ■Operational Conditions Column: Shim-pack GPC-804. Column temperature: 40℃ Detector: UV spectrophotometric detector (210nm) Fig.d. Column temperature: 40℃ Detector: Refractive index detector 80 Fig. Column temperature: 40℃ Detector: UV spectrophotometric detector (220nm) Fig.0mm i.×15cm) Mobile phase: n-Hexane/ethanol (2/1) Flow rate: 1.0 mL/min.×15cm) Mobile phase: Water/acetonitrile.0mm i.d. 167 Analysis of Alkylamine-Polyethylene Aducts ■Operational Conditions Column: Shim-pack CLC-ODS (6.0mm i.d.× 30cm. Column temperature: 50℃ Detector: UV spectrophotometric detector (254nm) Fig. 803.0 mL/min. 168 Analysis of Polyoxyethylene Octylphenyl Ether ■Operational Conditions Column: Shim-pack CLC-SIL (6.5 mL/min. 176 Determination of EDTA ■Operational Conditions Column: STR ODS-Ⅱ (4.d. Column temperature: 40℃ Detector: UV spectrophtometric detector (258nm) 81 .×10cm) Mobile phase: 2.×15cm) Mobile phase: 10mM phosphate buffer solution (pH 2. Column temperature: 50℃ Detector: Reractive index detector Fig. 173 Determination of Impurities in Terephthalic acid ■Operational Conditions Column: Shim-pack CLC-ODS (6.6mm i.0 mL/min.d. 175 Determination of Borate in Plating Solution ■Operational Conditions Column: Shim-pack SCR-101H (7. 172 Determination of Irganox 1076 in Rubber ■Operational Conditions Column: Zorbax ODS (4.0mM phthalic acid and 1.×15cm) Mobile phase: 15mM inclding tributylamines 10mM phosphate aqueous solution (pH 7) 5/acetonitrile 1 Flow rate: 1.5 mL/min.8 mL/min.6mm i.d.5 mL/min. 4-Carboxybenzaldehyde ２.5mM tris (hydroxymethyl) aminomethane Flow rate: 1.6) /acetonitrile (4/1) Flow rate: 1.0mm i.d.6mm i. Column temperature: 40℃ Fe-EDTA Borate Fig. Column temperature: 40℃ Detector: Refractive index detector Fig.d. and 802 (8. Column temperature: 65℃ Detector: UV spectrophotometric detector (230nm) Fig. p-Toluic acid Fig.lrganox1076 Fig.0 mL/min.d. 802. 174 Determination of Chlorine in Concrete Additive ■Operational Conditions Column: Shim-pack IC-A1 (4.0 mL/min.×15cm) Mobile phase: Methanol/tetrahydrofuran (9/1) Flow rate: 1. 171 Analysis of Isoprene Rubber ■Operational Conditions Column: Shim-pack GPC-804.0mm i.× 30cm.×30cm) Mobile phase: 5mM perchloric acid aqueous solution Flow rate: 0. Column temperature: 40℃ Detector: UV spectrophotometric detector (280nm) Cl− ■Peaks １. each) Mobile phase: Tetrahydrofuran Flow rate: 1.9mm i. 1M phosphate buffer solution (pH 2.0mm i.0mM Hydroxyacetic benzoate Flow rate: 1.5 mL/min.d.5 mL/min. 178 Determination of Monovalent Cations in Soil Water ■Operational Conditions Column: Shim-pack IC-C1 (5mm i. Cl− ２.×15cm) 0. SO42− Fig. Na＋ ２. NO2− ３. NO3− ４. Column temperature: 40℃ Detector: UV spectrophotometric detector (265nm) Sample pretreatment: Concentrated with an automatic sample pretreatment system . Br− ２.5 mL/min. Column temperature: 40℃ Detector: UV spectrophotometric detector (210nm) 82 Fig. NH4＋ ３.d.Environmental Pollutants ■Peaks １. NO3− Phenol Fig. Column temperature: 40℃ Detector: Conductivity detector ■Peaks １. 179 Determination of Bromine and Nitric Acid ■Operational Conditions Column: Shim-pack IC-A1 (4.d.×15cm) Mobile phase: 5mM nitric acid aqueous solution Flow rate: 1. K＋ Fig. 180 Determination of Phenol in Water ■Operational Conditions Column: Shim-pack CLC-ODS (6. Column temperature: 40℃ Conductivity detector Detector: ■Peaks １.5 mL/min.1)/acetonitrile (3/1) Mobile phase: Flow rate: 1. 177 Determination of Inorganic Anion in Rain Water ■Operational Conditions Column: Shim-pack IC-A3 Mobile phase: 8.×15cm) Mobile phase: 10mM phosphate buffer solution (pH 6.8) Flow rate: 1.6mm i. use the kit below. 5pcs.6×200 0. Available to 1.3 i. Number Pipe 0.×o.8×1.d.6F Description Cat. titanium.6×500 Cat. 228-18565-84 by stainless plumbing parts Ferrule1.×1. Maxium pressure is 250kgf/cm2 when connecting to 0.6A Number ① Male Nut 1. Connector Plumbing Parts Male Nut 1.6FN Coupling1. No.6F 2pcs.6×400 0. Adapter1. PEEK (Inc.6MN Female Nut 1.6U Union1.6MN 2pcs.6×200 0. No 228-22301-00 228-22302-00 228-22303-00 228-22304-00 228-22305-00 228-22801-00 228-22802-00 228-22803-00 228-22804-00 228-22805-00 To connect this type of column.6mm o.3×1. No.5mm i.6×500 0.6F 228-16000-85 10 83 .6A 228-16005-03 1 ⑤ Union1. Column Connection Kit C ASSY 228-16058-91 (For new-new type connection) Male Nut 1.6×300 0. Pre-cut Stainless Pipe Description Male Nut.6C 228-16004-03 1 ④ Adapter1. pipe (SS 316) is cut at the size of easily use and mechanical-forged smoothly in both edges.6×100 0.6FN 228-16002-84 10 ③ Coupling1. No.6U 228-00034 1 ⑥ Union1. This 1. Ferrule 1.6×400 0.5 Parts for Flow line ■Plumbing parts (Connecting Parts) Cut and sharpen not necessary Forged pipe Fingertightening connector You can connect the column by tighening this connector.6U(X) Ferrule1.6×100 0.6×300 0. teflon.3×1.8×1.) ● Connecting i.d.×length (mm) 0.3×1. not by wrench.6C Union1.6F 228-16000-84 3 ⑦ Ferrule1.3×1. PEEK pipe.d. Description Cat.8×1.×25cm 1pc. This PEEK connector which is superior in chemical compatibility and mechanical strength is suitable for biological LC.6U(X) 228-01356 1 ⑦ Ferrule1.d.6mm i.d. PEEK etc.6 o.8×1. tefzel.d.d. pipe such as stainless.3×1.6MN 228-16001-84 10 ② Female Nut1.8×1.6F SS pipe Male Nut Column Cat. o. 1.6FN 1.d.6U 1. 670-12327 .6MN 1. 1. 1.6F 1. 0. It is possible to cut the pipe with the cutter attached as the length as you like.d. to 1. comparing with the case of filing.6F 1. 250 length 1 Each 1 1 ｝ 〔Kit〕 Description Cat.6P Longbush.6U(X) 1. (Do not forget to file the end of the pipe. 1.d.1.) Possible to cut from 0. Use the pipe once filing the end of the stainless pipe smooth and checking with a magnifying glass carefully. We can look carefully with a High magnifying power glass made of noncolour lense. Coupling 1.6mm o. No.4 Plug.6MN 1.6FN 1. o. connectors and tools needed to HPLC plumbing parts.6FN 1. No. 670-18802 Cat.LC plumbing part kit This kit put in a prastic case consists of pipes. flat) Miniture vice Number Notes 10 20 2 2 2 For Rheodyne injector 4 5 2m i. Description A magnifying glass. 1. 7010-010 PTFE ferrule.6F-T PTFE tube.3 and 0.d. 1.d. 20 times Subscription Piping cutter 84 Cat.6U Newtype ASSY Type 228-16053-91 Union 1.6MN Ferrule.6 Pipe cutter File (triangle. No. 1. You can cut easily and the end of the pipe is smooth.6C-0.d.4 Each 2m i. 0.d.6U(X) Newtype ASSY 228-16054-91 Column connector kit 228-17943-92 ③ Coupling ⑦ Ferrule ① Male Nut ④ Adapter ⑦ Ferrule ① Male Nut ② Female Nut ⑤ Union ⑦ Ferrule ② Female Nut ⑥ Union ⑦ Frrule ② Female Nut ① Male Nut ⑦ Ferrule Products Name 1.6 Pre-cut pipe.6F Coupling. hold this in the miniture vice and file the end of this smooth.6MN 1.8 4 i. Description LC plumbing part kit Cat.6A 1. 0. No.6F 1.3mm i. 228-24209-91 ⑨ ⑬ ⑪ ⑧ ⑫ ⑩ LC Connecting Part Kit Mark ① ② ③ ④ ⑤ ⑥ ⑦ ⑧ ⑨ ⑩ ⑪ ⑫ ⑬ Description Male Nut.6F 1. 7010-011 Ferrule.d.6C 1. o.6 SS pipe.6A ASSY 228-16052-91 Union 1.6C ASSY 228-16051-91 Adapter 1.3.6F Number 1 2 2 1 2 1 1 1 2 2 1 2 2 2 2 Checking the end of pipe As if cutting a wire by wrench Cut the stainless pipe easily Holding and cutting the pipe like wrench by cutter in the LC plumbing part kit. After cutting the pipe. we can prevent plugging the pipe. 6×0. dichloromethane. Description Plumbing parts (Connecting parts) kit for inert LC Cat.d.25φ→∼200kgf/cm2 (19. 85 .50φ →∼150kgf/cm2 (14. ● The solvent when using PEEK resin Although PEEK resin has high mechanical strength and thus is considered as optimal plastic material for HPLC. Coupling made with PEEK ( ) ⑥ PEEK tube set (228-33406×2pcs.75φ →∼100kgf/cm2 (9. 1m) ＊There are two types: male nut・ferrule separation type and joint type. Male Nut・Ferrule separate type ( ④ Stop joint PEEK (228-28090×2pcs. 0.6MN-2 PEEK＊ 228-33213×5pcs. Preparing for the case of PEEK tube is broken. tetrahydrofuran(THF).6C PEEK 228-25014×2pcs.(Refer to P.25×500 straight ⑥-2 PEEK tube 1. When using PEEK tube with aqueous solution like IC. acetone There is no problem of using methanol and acetonitriles. use joint type under normal pressure. but use these solvents within the pressure below.6MPa) i. 228-33285-91 Component of Plumbing parts (Connecting parts) kit for Non-metal LC ① Male Nut 1.) ⑥-1 PEEK tube 1. dichloroacetic acid. there are some restrictions to use. concentrated nitric acid. dimethyl sulfoxide (DMSO).8MPa) ● Each PEEK tube showed in this page includes FEP tube for protection.6×0. be sure to put FEP tube for the security.6MPa) Acetonitriles: i. use separationtype. Make sure that particularly the solvents below are NOT used.25×500 L type ⑥-3 FEP tube NFL016 (For protect PEEK tube. chloroform.125φ/0.d. No. PEEK Plug) ) ② Ferrule 1. 0. 0. Concentrated sulfuric acid.d. Male Nut・Ferrule separate type ( ⑤ Clean cut 228-33930×1pcs.7MPa) i.■PEEK plumbing parts Use this parts for maintenance Non-metal LC system and system up. A pressing cutter for PEEK tube ( ) ) ③ Male connector 1.6F PEEK＊ 228-31638×10pcs. Methanol: Each diameter∼200kgf/cm2 (19.83) When using PEEK tube with organic solvent type mobile phase. ×3000mm) 228-33833-92 PEEK tube (0.d.d.PEEK plumbing parts (Not included in Plumbing Parts Kits) Description Cat.d. so that this set is not necessary when installing.25 i. 86 . nut・ferrule each 5 (A spare for Rheodyne 9725/i)＊2 Filter for protecting a column (Put between injector and column) PEEK tube (0.6 o.6 o.×3000mm) 228-33833-94 Sample loop 100μL 228-32651-16 Rheodyne injector for 9725/i 200μL 228-32651-17 Rheodyne injector for 9725/i 500μL 228-32651-18 Rheodyne injector for 9725/i 1mL 228-32651-19 Rheodyne injector for 9725/i ＊1 Use this with chemical reaction detection system etc. No.6C PEEK 228-25013 Female T joint＊1 Stop joint PEEK 228-28090 Plug Tubing cutter 228-32930-01 Press cutter for PEEK tube Replacement blade 228-32930-02 PEEK Tube set 228-33406-91 Inline filter PEEK 228-32939 Frit (1pcs.6 o.d.) 228-32744 Spare filter for inline filter Frit (5pcs.d.×1.50 i.×1.d. Male Nut 1.125 i.) 228-33691-91 Spare filter for inline filter Rheflex fitting set 228-32651-41 Inc.×1.6F PEEK (includes 3pcs. set for PEEK tube of Rheodyne 9725/i.6MN-2 PEEK 228-33213 Male nut・Ferrule separation type Contents Ferrule 1.d.6 o.) 228-33513-91 Male nut・Ferrule separation type Male connector 1.×3000mm) 228-33833-93 PEEK tube (0.×1. "Rheflex" stands for Fex fitting made in Rheodyne. ＊2 Nut・Ferrule 5pcs.×3000mm) 228-33833-91 PEEK tube (0.d.6C PEEK 228-25014 Coupling 3way joint 1.75 i. Rheodyne 9725/i includes nut ・ferrule 4pcs. ■Disposable Vial ● Mass products made by plastic Suitable for the disposable of many samples ● Septa is attached in a cap Not necessary to set up septa ● Special patterned ditch on the septa The trouble such as the tears of the septa and the plugging of the syring hole doesn't occur.2 buffer) ● Amino acid mobile phase kit Na type consists of mobile phase for amino acid analysis composed of protein (2 pcs. No. ■Specification Injector SIL-10A. 87 . 228-31600-91 Caution) When using disposable vial with a sample of organic solvent type. ● Analytical kit OPA reagent is a set of reaction reagent (2 kinds) and can be used in case of Na type and Li type.2N-sodium citric acid Capacity 1L Usage A solution (No.15N-lithium citric acid Basic buffer O-phthalic aldehyde 1L×3pcs. suitable for high sensitive analysis. 1L A solution (No.1 buffer) Amino acid mobile phase kit Li type AA-MB(Li) AA-MC(Li) 0. the shape of the bottom is arranged not to leave air bubbles. ● As strictly selected grade reagent is used. Septa) Polypropylene (Case) Cat. SIL-10AXL Maxium amount 1mL Minium amount of sample (10μl inject) 50μ Materials Polyethylene (Cap. ● Amino acid mobile phase kit Li type consists of amino acid mobile phase and column clean up solution. Bottle name AA-MA(Na) Contents 0.15N-lithium citric acid 1L×3pcs. Also.1 buffer) Reaction reagent A Reaction reagent B Amino acid mobile phase kit Na type 228-21195-95 Amino acid mobile phase Li type A solution 228-21195-97 Amino acid analytical kit OPA reagent 228-21195-93 C solution (column clean up solution) B solution (No. SIL-6B.) and column clean up solution. SIL-9A.2N-lithium hydroxide 1L 500mL C solution (column clean up solution) AA-MA(Li) AA-RA AA-RB 0. No.1 buffer) 228-21195-94 AA-MB(Na) AA-MC(Na) 0. ● V bottom which solutions don't leave in The amount of the sample is available in the range of 50μl to 1ml. dilute and pH adjusting is not necessary.2N-sodium hydroxide 1L 500mL B solution (No. Mobile phase kit Analysis kit OPA reagent ■Classification of analytical kit Description Cat.2N-sodium citric acid 0.1 buffer) A solution (No.3N-lithium citric acid 0.6N-sodium citric acid 0. 500mL 500mL A solution (No.■Shimadzu Amino Acid Analysis kit for amino acid analytical system ● As both mobile phase and reaction solutions are prepared to be optimized of analytical conditions. be sure to check whether there are disturbing peak or not to do branktest.2 buffer) Amino acid mobile phase kit A solution 228-21195-96 AA-MA(Na) AA-MA(Li) 0. strong Filter. Features ● Small disk shape disposable filter. ● Filtration can be with high flow and low presure. Therefore the column you use can be kept long.45 670-12540-23 acid and strong alkarine.25A 25 0.45 670-12540-13 Filter.45 670-12540-03 particles. ● As Filter. lock attached and you can filtrate with easily operation and no trouble. (Inc. (halftransparent) ● Superiority Filter.45 670-12540-21 divided into each 10pcs. each 100pcs. Ion chromato type ● Sterilization.4AI 4 0. clean can be kept.45 670-12540-22 is solvent and chemical compatibility ● Suitable for the pretreatment of semi-analytical sample which are an object of solvent. No.13N 13 0.45 670-12540-12 ● Suitable for the pretreatment of the sample for ion chromatography analysis. ● It is good for air vent filter. Filter. ● Composed of PTFE membrance and a Organic type polypropylenehousing.45 670-12540-02 for the pretreatment of the sample for HPLC analysis and protein analysis which are an object of aqueous and alcohol solution.45 670-12540-11 ● Composed eliminated of a de-ionized hydrophilic membrance and a polypropylenehousing (light blue) Filter. Filter. the change of the property.25N 25 0. ● The amount of the liquid left in this filter is quite small.13A 13 0.■Disposable Filter This disposable filter makes it possible to eliminates precipitate materials and minute particles in a sample. 670-12540-01 Features ● Composed of a hydrophilic membrane and a polypropylenehousing (blue). ● There are many kinds of filter including ion chromatography as follows.13AI 13 0. 4A Shape Diameter (mm) Pore Size (μm) 4 0. You can prevent the plugging of the column.) 88 . ● Suitable Filter.4N 4 0.45 Cat. and the trouble of the injector and the micro syringe.25AI 25 0. ● Ruar Series Description Aqueous type Filter. 07 0.)/case 100 Stuff＊1 4AⅠ 13mm Ruar slip E 100 Ruar slip A.45 0.45 0.07 0.Chemical Compatibility Esters Chemical name Hydrogen carbide Ketones △ △ ○ ○ × △ × △ × △ △ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ Oil group Halogenation hydrogen carbide Glacial acetate acid Acetate acid 90％ 〃   30％ 〃   10％ Hydrochloric acid Conc 〃    6N Silfuric acid Conc 〃  6N Nitric acid Conc 〃  6N Ammonia water 6N 〃 3N Calcium hydroxide 3N Sodium hydroxide 5N 〃 3N Methanol Ethanol Propanol Isopropanol Butanol Amyl alcohol Ethylene glycol Propylene glycol Glycerine Ethyl ether Isopropyl ether Dioxane Tetrahydrofuran Methyl esters Etyl esters Isopropyl esters Butyl esters Amyl esters Serosolve esters Organic type Others Ether Alcohols Alkaline Acid Chemical name ○ Can use △ Can use for short time Aqueous & Ion chromato type △ △ ○ ○ × × × × × × △ ○ ○ △ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ × × × × × × × × Acetone Cyclohexane Metyl etyl ketone Metyl isobutyl ketone Benzene Toluene Xylenes Etane dichloride Methylene chloride Chloroform Carbon tetrachloride Perchloroe etylene Trichloro etylene Freon TF Freon TMC Cotton-seed oil Lubrication oil Peanut oil Sesame oil Acetnitryl Aniline Gasoline Kerosene Dimethylsulfoxide Dimethylsulfoxide Terpene oil Pyridine Phenols (liquid) Hexane (dry) Formaldehyde 37％ Aqueous & Ion chromato type × × × × × × × × × × × × × × × ○ × ○ ○ × × ○ ○ × × ○ × × ○ ○ × Can NOT use Organic type ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ Specification 4mm Type aqueous Ion chromato type type Item Pore size 4A μm Organic type aqueous Ion chromato type type 4N 13A 13AⅠ 25mm Organic type 13N aqueous Ion chromato type type 25A 25AⅠ Organic type 25N 0.8 0.45 0.8 0.E 100 Ruar slip E 100 ＊1 OP＝Olefines type polymer.45 0.45 OP OP PTFE OP OP PTFE OP OP PTFE PP PP PP PP PP PP PP PP PP 8×18 8×18 8×18 18×22 18×22 18×22 29×24 29×24 29×24 0.45 0. PP＝Polypropylene.45 0.45 0.45 0.8 4 4 4 7 7 7 5 5 5 6 6 6 100 100 100 100 100 100 100 100 100 Less than 10 Less than 10 Less than 10 Less than 30 Less than 30 Less than 30 Less than 100 Less than 100 Less than 100 Ruar lock Ruar lock Ruar lock Ruar lock Ruar lock Ruar lock Ruar lock Ruar lock Ruar lock Filter Housing Size(φ×L) mm Available filtration area cm2 Maxium pressure kg/cm2 Maxium temperature ℃ Amount of the liquid left μL In Connecting parts Out Ruar slip Sterilization method＊2 E Number(pcs.E 100 89 . E＝Ethylene oxide gas Ruar slip E 100 Ruar slip A. PTFE＝Polytetrafluoroethylene ＊2 S＝AUTO clave.E 100 Ruar slip E 100 Ruar slip E 100 Ruar slip A.07 0. 6mm i.×15cm 228-17877-91 Shim-pack CLC-Phenyl（M） 4.0mm i.×15cm 228-17255-91 Shlm-pack CLC-ODS/H 4.×15cm 228-17874-91 Shim-pack CLC-C8（M） 4.6mm i.×15cm 228-17876-91 Shim-pack CLC-CN（M） 4.d.6mm i.33 Shim-pack BIO （T） Shlm-pack CLC Reference P.×25cm 228-17877-92 Shim-pack CLC-NH2 6.d.6mm i.d.6mm i.×15cm 228-00809-91 Shlm-pack CLC-C8（M） 4.d.d.6mm i.d.×10cm 228-18837-91 Shim-pack CLC-ODS（T） 4.d.6mm i.×25cm 228-17873-92 Shim-pack CLC-C8 6.d.×5cm 228-18257-91 Shim-pack WAX-2（T） 4.×5cm 228-18259-91 Shim-pack HPC-C2（T） 4.0mm i.No.0mm i.d.×25cm 228-17878-92 Shim-pack CLC-VMA 6.d.d.×15cm 228-00812-91 Shim-pack CLC-Phenyl（M） 4.d.×25cm 228-17875-92 Shjm-pack CLC-CN 6.d.×25cm 228-17874-92 Shim-pack CLC-TMS 6.×5cm 228-18260-91 Shim-pack HPC-C3（T） 4.d.d.×15cm 228-00807-91 Shlm-pack CLC-SIL（M） 4.×15cm 228-17872-91 Shim-pack CLC-SIL（M） 4.×15cm 228-00808-91 Shim-pack CLC-ODS（M） 4.6mm i.d.×5cm 228-18261-91 Shim-pack CLC-SIL 6.d.6mm i.×15cm 228-17873-91 Shjm-pack CLC-ODS（M） 4.d.6mm i. Shim-pack Amino-Na 6.d.d.d.×15cm 228-17875-91 Shim-pack CLC-TMS（M） 4.d.×15cm 228-00810-91 Shlm-pack CLC-TMS（M） 4.d.×25cm 228-00808-92 Shim-pack Amino P.6mm i.6mm i.0mm i.6mm i.6mm i.×15cm 228-00810-91 Shim-pack CLC-CN（M） 4.6mm i.57 P.6mm i.×15cm 228-16725-91 Shim-pack CLC-NH2（M） 4.d.0mm i.d.×15cm 228-17878-91 Shim-pack CLC-NH2（M） 4.24 P.6mm i.×25cm 228-17876-92 Shim-pack CLC-Phenyl 6.d.d.6mm i.6mm i.×5cm 228-18258-91 Shim-pack WCX-1（T） 4.d.×25cm 228-17872-92 Shim-pack CLC-ODS 6.d.0mm i.d.d.51 90 .0mm i.0mm i.0mm i.6mm i.6 Shim-pack Columns Column series Column name Dimension Cat.×15cm 228-18062-91 Shim-pack WAX-1（T） 4.6mm i.0mm i.×10cm 228-18837-91 Shim-pack Amino-Li 6. 5cm 228-18268-92 Shim-pack GPRC-SIL 8.5cm 228-18338-91 Shrm-pack GL-SIL 50mm i.×1cm 228-18268-91 Shim-pack G-NH2（8） 8.No.0mm i.5cm 228-24387-92 Shlm-pack Diol Shim-pack FLC Reference P.d.0mm i.d.×50cm 228-14775-92 Shim-pack Diol-300 7.×1cm 228-18262-91 Shimｰpack G-TMS（8） 8.41 P.54 91 .9mm i.d.×1.5cm 228-18270-92 Shim-pack GK-SIL 30mm i.0mm i.d.d.d.d.30 P.×5cm 228-13695-91 Shim-pack FLC-NH2 4.×1.0mm i.d.0mm i.6mm i.d.×1.×5cm 228-13696-91 Shim-pack G-SIL（4） 4.0mm i.×5cm 228-13694-91 Shim-pack FLC-C8 4.0mm i.d.d.0mm i.×7.d.×1.×1.0mm i.×1cm 228-18248-91 Shim-pack G-C8（8） 8.×5cm 228-13375-92 Shim-pack FLC-CN 4.d.×5cm 228-18339-91 Shim-pack G-ODS（4） 4.0mm i.d.9mm i.5cm 228-23465-92 Shim-pack GPRC-C8 8.6mm i.5cm 228-18246-92 Shim-pack GK-ODS 30mm i.24.5cm 228-24386-92 Shim-pack GPRC-TMS 8.0mm i.6mm i.d.×5cm 228-18322-91 Shim-pack G-C8（4） 4.d.d. Shim-pack Diol-150 7.×25cm 228-14775-91 Shim-pack Diol-150 7.×1cm 228-18266-91 Shim-pack G-CN（8） 8.×5cm 228-13375-91 Shim-pack FLC-SIL 4.×1.d.6mm i.×1.9mm i.d.0mm i.×1.d.5cm 228-18266-92 Shim-pack G-Phenyl（4） 4.5cm 228-18262-92 Shim-pack G-CN（4） 4.0mm i.9mm i.×7.d.0mm i.d.0mm i.Column series Column name Dimension Cat.×1.5cm 228-23462-92 Shim-pack GPRC-ODS 8.d. 29 Shim-pack G P.d.d.d.d.×1cm 228-18264-91 Shim-pack G-Phenyl（8） 8.5cm 228-18321-91 Shim-pack GL-ODS 50mm i.×25cm 228-14776-91 Shim-pack Diol-300 7.5cm 228-18264-92 Shim-pack G-NH2（4） 4.×1cm 228-18270-91 Shim-pack G-SIL（8） 8.0mm i.d.×1.d.×1.0mm i.0mm i.×1cm 228-18246-91 Shim-pack G-ODS（8） 8.×50cm 228-14776-92 Shim-pack FLC-ODS 4.d.5cm 228-18248-92 Shim-pack G-TMS（4） 4.6mm i.d. Column series Column name Dimension Cat.No. Shim-pack GPRC-NH2 8.0mm i.d.×1.5cm 228-24388-92 Shim-pack GPRC-CN 8.0mm i.d.×1.5cm 228-24389-92 Shim-pack GPRC-SIL（K） 3.0mm i.d.×7.5cm 228-23462-93 Shim-pack GPRC-SIL（L） 50mm i.d.×5cm 228-23462-92 Shim-pack GPRC-ODS（K） 30mm i.d.×7.5cm 228-23465-93 Shim-pack GPRC-ODS（L） 50mm i.d.×5cm 228-23465-94 Shim-pack GPC-801 8.0mm i.d.×30cm 228-20803-91 Shim-pack GPC-802 8.0mm i.d.×30cm 228-20804-91 Shim-pack GPC-8025 8.0mm i.d.×30cm 228-20805-91 Shim-pack GPC-803 8.0mm i.d.×30cm 228-20806-91 Shim-pack GPC-804 8.0mm i.d.×30cm 228-20807-91 Shim-pack GPC-805 8.0mm i.d.×30cm 228-20808-91 Shim-pack GPC-806 8.0mm i.d.×30cm 228-20809-91 Shim-pack GPC-80M 8.0mm i.d.×30cm 228-20810-91 Shim-pack GPC-807 8.0mm i.d.×30cm 228-20811-91 Shim-pack GPC-800P 4.6mm i.d.×1cm 228-20812-91 Shim-pack GPC-801C 8.0mm i.d.×30cm 228-20803-92 Shim-pack GPC-802C 8.0mm i.d.×30cm 228-20804-92 Shim-pack GPC-8025C 8.0mm i.d.×30cm 228-20805-92 Shim-pack GPC-803C 8.0mm i.d.×30cm 228-20806-92 Shim-pack GPC-804C 8.0mm i.d.×30cm 228-20807-92 Shim-pack GPC-805C 8.0mm i.d.×30cm 228-20808-92 Shim-pack GPC-806C 8.0mm i.d.×30cm 228-20809-92 Shim-pack GPC-80MC 8.0mm i.d.×30cm 228-20810-92 Shim-pack GPC-807C 8.0mm i.d.×30cm 228-20811-92 Shim-pack GPC-800CP 4.6mm i.d.×1cm 228-20812-92 Shim-pack GPC-801D 8.0mm i.d.×30cm 228-20803-93 Shim-pack GPC-802D 8.0mm i.d.×30cm 228-20804-93 Shim-pack GPC-8025D 8.0mm i.d.×30cm 228-20805-93 Shim-pack GPC-803D 8.0mm i.d.×30cm 228-20806-93 Shim-pack GPC-804D 8.0mm i.d.×30cm 228-20807-93 Shim-pack G Shim-pack GPC 92 Reference P.54 P.38 Column series Shim-pack GPC Shim-pack GRD Column name Dimension Cat.No. Shim-pack GPC-805D 8.0mm i.d.×30cm 228-20808-93 Shim-pack GPC-806D 8.0mm i.d.×30cm 228-20809-93 Shim-pack GPC-80MD 8.0mm i.d.×30cm 228-20810-93 Shim-pack GPC-807D 8.0mm i.d.×30cm 228-20811-93 Shim-pack GPC-800DP 4.6mm i.d.×1cm 228-20812-93 Shim-pack GPC-2001 20.0mm i.d.×30cm 228-23342-91 Shim-pack GPC-2002 20.0mm i.d.×30cm 228-23342-92 Shim-pack GPC-20025 20.0mm i.d.×30cm 228-23342-93 Shim-pack GPC-2003 20.0mm i.d.×30cm 228-23342-94 Shim-pack GPC-2000P 8.0mm i.d.×30cm 228-20812-94 Shim-pack GPC-2001C 20.0mm i.d.×30cm 228-23343-91 Shim-pack GPC-2002C 20.0mm i.d.×30cm 228-23343-92 Shim-pack GPC-20025C 20.0mm i.d.×30cm 228-23343-93 Shim-pack GPC-2003C 20mm i.d.×30cm 228-23343-94 Shim-pack GPC-2000CP 8.0mm i.d.×30cm 228-20812-95 Shim-pack GRD-ODS 4.0mm i.d.×25cm 228-16557-91 Shim-pack HPC-C2 4.0mm i.d.×5cm 228-17792-91 Shim-pack HPC-C3 4.0mm i.d.×5cm 228-17793-91 4.6mm i.d.×15cm 228-23460-91 4.6mm i.d.×25cm 228-23460-92 6.0mm i.d.×15cm 228-23460-93 4.6mm i.d.×15cm 228-24463-91 4.6mm i.d.×25cm 228-23463-92 6.0mm i.d.×15cm 228-23463-93 4.6mm i.d.×15cm 228-24376-92 4.6mm i.d.×25cm 228-24376-92 6.0mm i.d.×15cm 228-24376-92 4.6mm i.d.×15cm 228-24337-91 4.6mm i.d.×25cm 228-24377-92 6.0mm i.d.×15cm 228-24377-93 4.6mm i.d.×15cm 228-24378-91 Shlm-pack HPC Reference P.38 P.50 P.47 Shim-pack HRC-SIL Shim-pack HRC-ODS Shim-pack HRC Shim-pack HRC-C8 Shim-pack HRC-TMS Shim-pack HRC-NH2 P.21 93 Column series Column name Dimension Cat.No. 4.6mm i.d.×25cm 228-24378-92 6.0mm i.d.×15cm 228-24378-93 4.6mm i.d.×15cm 228-24379-91 4.6mm i.d.×25cm 228-24379-92 6.0mm i.d.×15cm 228-24379-93 Shim-pack IC-A1 4.6mm i.d.×10cm 228-17733-91 Shim-pack IC-A2 5.0mm i.d.×5cm 228-17735-91 Shim-pack IC-C1 5.0mm i.d.×15cm 228-17737-91 Shim-pack IC-GA1 4.6mm i.d.×1cm 228-17734-91 Shim-pack IC-GA2 4.0mm i.d.×1cm 228-17736-91 Shlm-pack IC-GC1 4.0mm i.d.×1cm 228-17738-91 Shim-pack IC-PCI 8.0mm i.d.×5cm 228-17744-91 Shim-pack IC-A1（S） 2.0mm i.d.×15cm 228-33400-91 Shim-pack IC-A3（S） 2.0mm i.d.×15cm 228-33366-91 Shim-pack IC-C3（S） 2.0mm i.d.×10cm 228-33367-91 Shim-pack IC-CI PEEK 4.6mm i.d.×10cm 228-33497-91 Shim-pack IC-GC1 PEEK 4.6mm i.d.×9cm 228-33497-92 Shim-pack ISA-07/S2504 4.0mm i.d.×25cm 228-09699-91 Guard Column ISA 4.0mm i.d.×5cm 228-00823-91 Shim-pack ISC-05/S0504 4.6mm i.d.×3.8cm 228-09700-91 Shim-pack ISC-07/S1504 4.0mm i.d.×15cm 228-09328-91 Shim-pack ISC-07/S1504Li 4.0mm i.d.×15cm 228-00796-91 Shim-pack ISC-30/S0504 4.0mm i.d.×5cm 228-14206-91 Shim-pack ISC-30/S0504Li 4.0mm i.d.×5cm 228-00821-91 Guard Column ISC-07 4.0mm i.d.×5cm 228-00802-91 Guard Column ISC-07Li 4.0mm i.d.×5cm 228-00797-91 Reference Shim-pack HRC-NH2 Shim-pack HRC Shim-pack-HRC-CN Shim-pack IC P.46 Shim-pack ISA Shim-pack ISC 94 P.21 P.33 P.33 ×50cm 228-12813-05 Shim-pack MBC-C8 1.×10cm 228-20759-92 Shim-pack PA-CM 8.×25cm 228-24384-92 Shim-pack PA-DEAE 8.×10cm 228-20760-91 Shim-pack PA-CM 20mm i.d.×50cm 228-12811-05 Shim-pack MBC-SIL 1.×100cm 228-12811-10 Shim-pack MBC-ACN 1.×25cm 228-12813-02 Shim-pack MBC-ACN 1.×1cm 228-20764-91 Shim-pack PAG-SP 8.d.d.×50cm 228-12814-05 Shim-pack MRC-SIL 6.×1cm 228-20762-91 Shim-pack PAG-QA 8.0mm i.d.×25cm 228-23461-92 Shim-pack MRC-ODS 6.×25cm 228-24383-92 Shim-pack MRC-CN 6.d.21 Shim-pack PA P.d.×10cm 228-20758-92 Shim-pack PA-QA 8.d.d.×25cm 228-24382-92 Shim-pack MRC-NH2 6.d.0mm i.0mm i.0mm i.d.d.0mm i.d.0mm i.0mm i.d.0mm i.29 Shim-pack-MRC P.0mm i.0mm i.×1cm 228-20763-91 Shim-pack PAG-CM 8.d.0mm i.d.d.0mm i.d.×10cm 228-20758-91 Shim-pack PA-DEAE 20mm i.0mm i.0mm i.0mm i.×25cm 228-23461-94 Shim-pack PRC-SIL（L） 50mm i.×50cm 228-12812-05 Shim-pack MBC-SIL 1.d.×10cm 228-20760-92 Shim-pack PA-SP 8.33 Shim-pack PAG Shim-pack-PRC Reference P.0mm i.d.d.×10cm 228-20759-91 Shim-pack PA-QA 20mm i.×25cm 228-12814-02 Shim-pack MBC-C8 1.d.Column series Column name Dimension Cat.×25cm 228-12812-02 Shim-pack MBC-ODS 1.d.d.×1cm 228-20765-91 Shim-pack PRC-SIL 20mm i.d.0mm i.×25cm 228-24381-92 Shim-pack MRC-TMS 6.d.×25cm 228-23464-93 Shim-pack MBC P.d.×10cm 228-20761-91 Shim-pack PA-SP 20mm i.×25cm 228-23461-93 Shim-pack PRC-SIL（K） 30mm i.0mm i.d.33 P.×25cm 228-23464-92 Shim-pack MRC-C8 6.×25cm 228-23461-91 Shim-pack PRC-ODS 20mm i.0mm i.d.×10cm 228-20761-92 Shim-pack PAG-DEAE 8.0mm i.d.d.d.×25cm 228-23461-95 Shim-pack PRC-SIL（H） 20mm i.0mm i. Shim-pack MBC-ODS 1.0mm i.No.21 95 . d.×25cm 228-00814-91 Shim-pack PREP-SIL（H） 20mm i.d.×25cm 228-18320-91 Shim-pack PREP 96 Reference P.d.d.×25cm 228-23464-94 Shim-pack PRC-ODS（L） 50mm i.21 P.×25cm 228-24382-93 Shim-pack PRC-TMS（H） 20mm i.d.d.×25cm 228-00816-91 Shim-pack PREP-C8（H） 20mm i.d.d.d.d.d.×25cm 228-17880-91 Shim-pack PREP-SIL（H）･Kit 4.×25cm 228-24384-93 Shim-pack PRC-CN（H） 20mm i.d.d.×25cm 228-23464-95 Shim-pack PRC-ODS（H） 20mm i.×25cm 228-17883-91 Shim-pack PREP-CN 20mm i.No.d.×25cm 228-24384-91 Shim-pack PREP-SIL 20mm i.×25cm 228-17886-91 Shim-pack PREP-SIL（K） 30mm i.d.×25cm 228-17881-91 Shim-pack PREP･ODS（H）･Kit 4.d.×25cm 228-18319-91 Shim-pack PREP-SIL（L） 50mm i.d.×25cm 228-24383-93 Shim-pack PRC-NH2（H） 20mm i.×25cm 228-17885-91 Shim-pack PREP-NH2 20mm i.×25cm 228-17884-91 Shim-pack PREP-Phenyl 20mm i.d.d.d.d.d.Column series Shim-pack-PRC Column name Dimension Cat.×25cm 228-00817-91 Shim-pack PREP-TMS（H） 20mm i.d.×25cm 228-17879-91 Shim-pack PREP-NH2（H） 20mm i. Shim-pack PRC-ODS（K） 30mm i.d.6mm i.×25cm 228-00815-91 Shim-pack PREP-ODS（H） 20mm i.×25cm 228-00819-91 Shim-pack PREP-Phenyl（H） 20mm i.×25cm 228-24381-93 Shim-pack PRC-C8（H） 20mm i.×25cm 228-18274-91 Shim-pack PREP-ODS（L） 50mm i.×25cm 20mm i.d.×25cm 228-23464-91 Shim-pack PRC-C8 20mm i.×25cm 228-17887-91 Shlm-pack PREP-ODS 20mm i.d.×25cm 228-24383-91 Shim-pack PRC-CN 20mm i.×25cm 228-24382-91 Shim-pack PRC-NH2 20mm i.×25cm 228-00818-91 Shim-pack PREP-CN（H） 20mm i.6mm i.×25cm 228-17888-91 Shim-pack PREP-C8 20mm i.×25cm 20mm i.d.d.×25cm 228-24381-91 Shim-pack PRC-TMS 20mm i.×25cm 228-18273-91 Shim-pack PREP-ODS（K） 30mm i.d.×25cm 228-17882-91 Shim-pack PREP-TMS 20mm i.d.d.d.28 .d. 0mm i.×15cm 228-17269-91 Shlm-pack SBC-SIL 2.×30cm 228-07730-93 Shim-pack SCR-102H 8.0mm i.×5cm 228-16365-91 Shim-pack WCX-1 4.0mm i.d.×5cm 228-17892-91 Guard Column SCR（H） 4.d.d.41 Shim-pack WAX Shim-pack WCX Reference P.5mm i.×5cm 228-16366-91 Shim-pack SCR Shim-pack SPC P.0mm i.×1cm 228-17990-91 Shim-pack WAX-1 4.d.0mm i.d.d.33 97 .d.×1cm 228-18838-91 Shim-pack SPC-AE1 4.49 P.d.×5cm 228-17891-91 Guard Column SCR（P） 4.d.d.d.0mm i.×3cm 228-33713-91 Shim-pack SPC-RP2 4.30 P.d.×30cm 228-17893-91 Guard Column SCR（N） 4.9mm i.9mm i.×15cm 228-17270-91 Shim-pack SCR-101N 7.0mm i.0mm i.0mm i.9mm i.Column series Shim-pack SBC Column name Dimension Cat.×30cm 228-07730-92 Shim-pack SCR-101C 7.×5cm 228-17924-91 Shim-pack SPC-RP3 4.×15cm 228-17268-91 Shim-pack SBC-C8 2.0mm i.d.×5cm 228-09619-92 Guard Column SCR（C） 4.5mm i.0mm i.33 P.d. Shim-pack SBC-ODS 2.No.×30cm 228-17890-91 Shim-pack SCR-101H 7.5mm i.d.d.6mm i.×5cm 228-16225-91 Shim-pack WAX-2 4.d.9mm i.×5cm 228-09619-93 Guard Column SCR-102（H） 6.d.×30cm 228-17889-91 Shim-pack SCR-101P 7.d. 7 Sample and data Compound α−AAA α・A・B・A γ−ABA Acenaphthene Acetaldehyde Acetaldehyde Acetaminophen Acetate Acetic acid Acetic acid Acetic acid Acetone Acetylsalicylic acid Acetylsalicylic acid Aceumlnophen Acrolein Adenine Adenosine Adenosine Adenylate kinase ADP ADP ADP ADP β−AiBA Ala Ala Ala Ala Ala Ala β−Ala β−Ala Albumin Albumin Amitriptyline AMP AMP AMP AMP Amygdalin α−AnBA Ans Arabinose Arg Arg Arg Arg Arg Arg Ascorbic acid Ascorbic acid Asn Asn Asn Asp Asp Asp Asp Asp Aspartame ATP ATP ATP ATP Atropine Bacitracin Benzaldehyde Benzanilide Benzene Benzene Benzoic acid Benzoic acid Benzoic acid 98 Page Figure 37 79 37 16 18 71 77 73 43 72 73 18 26 78 26 18 25 25 32 44 18 35 74 78 37 37 37 71 74 76 79 37 79 44 48 16 18 35 74 78 75 37 37 43 37 37 71 73 76 79 27 68 37 71 79 37 37 71 74 76 17 18 35 74 78 25 61 18 21 21 40 17 68 72 51 164 51 3 11 117 153 126 64 122 130 11 21 155 21 11 16 16 34 66 6 42 132 158 51 50 51 119 137 149 164 51 164 67 76 3 6 42 132 158 143 51 51 63 50 51 119 137 149 164 27 113 51 119 164 50 51 119 137 149 4 6 42 132 158 17 104 11 54 4 114 123 Compound Benzoic acid Benzothorium Chloride Berberine Berberine Big gastrin Biotin Biphenyl Blue dextran 2000 Borate Bovine scrum albumin Br− Br− Bradykinin Bromovalerylurea Bromovalerylurea BSA Butanol 2−butanone Butylaldehyde Butyraldehyde Ca2＋ Caffeine Caffeine Caffeine Caffeine Caffeine Caffeine Caffeine Caffeine Caffeine Calcium pantothenate Calcium pantothenate Camphor Car Carbamazepin Carbamazepin Carbonic anhydrase Carbonic anhydrase 4−Carboxybenzaldehyde Cd2＋ CDP CDP Cellobiose Chlorhexidine Chlorhexidine gluconate Chondroitin sulfate Chrorpheniramine maleate Chymotrypsin α−Chymotrypsinogen A α−Chymotrypsinogen A α−Chymotrypsinogen A α−Chymotrypsinogen A α−Chymotrypsinogen A α−Chymotrypsinogen A α−Chymotrypsinogen A α−Chymotrypsinogen A α−Chymotrypsinogen A Cinchonine cis−and trans−lsoadhumulone cis−and trans−lsocohumulone cis−and trans−lsohumulone Cit Cit Citrate Citric acid Citric acid Cl Cl− Cl− Cl− Cl− CMP CMP Co2＋ Page Figure 74 75 18 75 60 68 72 44 81 36 46 82 61 26 32 68 60 18 71 18 46 17 18 25 26 32 68 72 75 77 68 72 76 37 72 78 36 58 81 46 35 78 37 18 76 76 26 44 35 36 36 36 48 48 58 61 64 61 51 51 51 37 79 73 43 72 46 73 77 81 82 35 78 46 135 141 9 142 96 113 124 65 175 45 70 179 104 21 37 115 94 11 117 11 72 4 7 13 21 37 113 120 138 153 113 120 144 51 121 154 45 90 173 73 42 158 49 8 146 147 18 65 38 46 47 48 74 76 91 101 107 100 80 80 80 51 164 126 64 122 70 126 152 174 177 42 158 73 . S.) Ethosuximide Ethosuximide Ethyl acetate Ethyl butyrate Ethyl caproate Ethyl propionate Ethyl valerate Ethylbenzoate Ethylene glycol Ethyleneglycol Ethylparaben F− Fe−EDTA Fe2＋ Fe3＋ Fluocinolone acetonide Folic acid Formaldehyde Formaldehyde Formic acid Formic acid Fructose Fructose Fructose Page Figure 36 60 18 46 35 78 46 79 76 37 37 25 32 36 44 44 48 48 61 68 25 60 68 27 26 56 56 26 56 77 26 26 60 51 51 31 60 60 44 26 27 32 32 32 43 26 32 75 75 78 72 78 25 25 25 25 25 32 60 44 68 46 81 46 46 76 68 18 71 43 73 27 37 43 45 97 11 71 42 158 73 164 149 50 51 16 34 43 65 66 74 76 101 115 16 94 114 24 22 87 87 22 87 153 22 22 94 81 81 32 95 96 66 23 27 35 35 35 62 21 37 139 138 156 121 154 15 15 15 15 15 33 94 69 114 70 176 73 73 148 113 11 117 64 130 26 49 62 Compound Fructose Fructose Fructose Fructose Fructose Fumaric acid Galactose Galactose Galactose GDP GDP Giucose Gln Gln Gln Globulin Glu Glu Glu Glu Glu Glu Glucose Glucose Glucose Glucose Glucose Glucose Glucose Glucose Glucose Glucose Glucose Glutamate dehydrogenase Gly Gly Gly Gly Gly Gly Gly−Gly−Ala Gly−Gly−Leu Gly−Tyr Glycerol Glycerol Glycerol β−Glycyrrhrtinic acid GMP GMP GTP GTP Guaninc Guanosine Guanosine Hemoglobin A Hemoglobin A Hemoglobin C Hemoglobin F Hemoglobin F Hemoglobin S Hemoglobin S Hesperidin Hesperodine Hexanaldehyde Hexanol Hexobarbital (I.) His His His His His His Histamine Homovanillic acid (HVA) Page Figure 65 65 73 73 73 43 37 43 73 35 78 37 37 71 79 64 37 37 71 74 76 79 27 43 43 43 65 65 65 65 73 73 73 44 37 37 71 74 76 79 61 61 44 27 43 43 77 35 78 35 78 25 25 32 35 36 35 35 36 35 36 17 74 18 60 78 37 37 71 74 76 79 79 79 109 111 127 128 129 64 49 63 129 42 158 49 51 119 164 108 50 51 119 137 149 164 26 61 62 63 109 110 111 112 127 128 129 66 50 51 119 137 149 164 103 103 65 26 62 63 150 42 158 42 158 16 16 34 39 44 39 39 44 39 44 5 133 11 94 154 50 51 119 137 149 164 163 161 99 .S.Compound Conalbumin Creatinine Crotonaldehyde Cs＋ CTP CTP Cu2＋ Cys Cysteine Cystine Cystine Cytidine Cytidine Cytochrome C Cytochrome C Cytochrome C Cytochrome C Cytochrome C Cytochrome C Cytochrome C Cytosine 1−Decanol Dehydroacetic acid Dexamethasone Dibutyl phthalate Dibutylphthalate Didecylphthalate Diethyl phthalate Diheptylphthalate Dihydrocodeine phosphate Dimethyl phthalate Dioctyl phthalate 1−Dodecanol DOMA DOPAC Dulcoside A β−Endorfin β−Endorfin Enolase Ergosterol Erythorbic acid Estradiol Estriol Estrone Ethanol Ethenzamide Ethenzamide Ethinylestradiol Ethofyline (I.) Ethofyline (I.S. Compound Human serum albumin HVA Hydrocortisone acetate HyLys Hypoxanthine Hypoxanthine Hypoxanthine Hypro IgG IgG Ile Ile Ile Ile Ile Ile IMP IMP Inosine Inosine Insulin Insulin Insulin Insulin B chain Insulin b chain iso−Butylparaben iso−Maltose Isomaltoheptaose Isomaltohexaose Isomaltononaose Isomaltooctaose Isomaltopentaose Isomaltose Isomaltotetraose Isomaltotriose K＋ K＋ K＋ α−Ketoglutaric acid α−Lactalbumin α−Lactalbumin Lactate Lactate dehydrogenase Lactic acid Lactic acid β−Lactoglobulin β−Lactoglobulin A β−Lactoglobulin B Lactose Lactose Lactose Leu Leu Leu Leu Leu Leu−Enkephalin Leu−Enkephalin Levulinic acid Li＋ lodoform Lou lrganox1076 Lys Lys Lys Lys Lys Lys Lys−Bradykinin Lysophosphatidylcholine (Lysolecithin) Lysozyme Lysozyme Lysozyme 100 Page Figure 44 51 27 37 18 74 78 37 44 58 37 37 71 73 76 79 18 74 18 74 60 60 61 61 60 68 73 65 65 65 65 65 65 65 65 46 46 82 43 36 58 73 44 43 73 44 36 36 27 37 65 37 37 71 73 76 61 61 43 46 76 79 81 37 37 71 74 76 79 61 25 35 36 36 65 81 24 51 6 132 157 51 67 92 50 51 119 137 149 164 6 132 6 132 95 96 104 104 95 114 129 112 112 112 112 112 112 112 112 71 72 178 64 45 90 126 66 64 130 65 45 45 26 49 109 50 51 119 137 149 103 104 64 71 145 164 172 50 51 119 137 149 164 104 12 38 46 47 Compound Lysozyme Lysozyme Lysozyme Lysozyme Lysozyme Lysozyme Lysozyme Lysozyme Lysozyme m−tolualdehyde Malate Malic acid Malic acid Malic acid Malonic acid Maltoheptaose Maltohexaose Maltopentaose Maltose Maltose Maltose Maltose Maltose Maltose Maltose Maltotetraose Maltotriose Mannitol Mannose Mastoparan 1−Me−His 3−Me−His Menthol Mercaptoalbumin Met Met Met Met Met Met Met−Enkephalin Met−Enkephalin Methacrolein Methyiparabon Methyl benzoate Methyl benzoate Methyl salicylate Methylbenzoate Methylephedrine Mg2＋ MHPG Mn2＋ Moltotriose Myoglobin Myoglobin Myoglobin Myoglobin Myoglobin Myoglobin Myoglobin Myoglobin Myoglobin Myoglobin Myoglobin Myoglobin N1−Acetylspermidine N1−Acetylspermine N−acetylprocainamide N−Acetylputrescine n−Butylbenzoate n−Butylparaben n−Hexyl benzoate n−Propyl benzoate n−Propylbenzene Page Figure 36 44 44 48 58 61 61 64 68 18 73 43 72 73 72 65 65 65 27 37 43 43 65 65 73 65 65 43 37 61 37 37 76 64 37 37 71 74 76 79 61 61 18 68 60 72 76 32 77 46 51 46 43 35 36 36 36 44 48 48 58 58 61 64 68 79 79 15 79 32 68 60 60 39 48 65 68 76 91 101 104 107 115 11 126 64 122 130 122 110 110 110 26 49 61 62 110 111 129 110 110 63 49 104 51 51 144 108 50 51 119 137 149 164 103 104 11 114 97 124 144 33 153 72 81 73 61 38 46 47 48 65 74 76 90 91 104 107 115 160 160 1 160 33 114 97 97 53 . Compound n−Propylbenzene n−Propylbenzoate n−Propylparaben Na＋ Na＋ Na＋ NAD NADH Naphazoline chloride Naphthalene Neostigmine sulfate Neurotensin NH＋4 NH＋4 NH＋4 NH3＋Et・NH2 Ni2＋ Nicotinamide Nicotinamide Nicotinamide Nicotinamide Nicotinamide Nicotinic acid Nicotinic acid Nicotinic acid NO2 NO−2 NO−3 NO−3 NO−3 NO−3 NO−3 Nonmercaptoalbumin Octanol 25−OH−D3 Orn Orn Ovalbumin Ovalbumin Ovalbumin Ovalbumin Ovalbumin Ovalbumin Ovalbumin Oxine Oxine p−Bromoacetanilide P−Et・NH2 p−Et・NH2 p−Hydroxy benzoic acid p−Ser p−Toluic acid Paeoniflorin Paeonol Pantothenic acid Pantothenic acid PEG 1000 PEG 4000 PEG 4000 PEG 6000 Pentanal Phc Phe Phe Phe Phe Phenacetin Phenobarbital Phenobarbital Phenol Phenol β−Phenylchalkone (I.) Phenytoin Phenytoin Page Figure 40 32 68 46 46 82 26 26 26 72 26 61 46 46 82 37 46 18 25 68 72 75 25 72 75 46 82 46 73 82 82 77 64 60 78 37 79 35 36 44 44 58 61 68 16 18 21 79 37 68 37 81 55 55 25 75 44 43 44 44 71 74 37 71 76 79 32 72 78 15 82 51 72 78 54 33 114 71 72 178 19 19 18 124 18 104 71 72 178 51 73 7 13 113 120 138 13 120 138 70 177 70 126 177 179 152 108 94 159 51 164 38 43 65 68 91 101 115 2 10 164 51 114 51 173 82 84 13 138 69 62 69 69 117 137 50 119 149 164 37 121 154 1 180 80 121 154 Compound Phosphatidylcholine (Lecithin) Phosphoric acid Phridoxine PO43− Polyethylene glycol Polyethylene glycol Polysaccharides Polystyrene 1.100K Polystyrene 1.800 Polystyrene Mw 300 Polystyrene Mw 42. 9.w.6K Polystyrene M.120K Polystyrene Mw 2.S.35K Polystyrene 115K Polystyrene 33K Polystyrene 410K Polystyrene 7.800 Pre Primidone Primidone Pro Pro Pro Pro Pro Pro Propionaldehyde Propionic acid Propranolol Propylaldehyde Pullulan Pullulan Putrescine Pyridoxine Pyridoxine Pyridoxine Pyridoxine hydrochloride Pyroglutamic acid Pyroglutamic acid Pyruvic acid Quinine Rafinose Ramnose Rb＋ Rebaudioside A Rebaudioside A Rebaudioside C Rhamnose Ribofiavin Ribofiavin phosphate Riboflavin Riboflavin Riboflavin phosphate Riboflavin phosphate Riboflavine Ribonuclease Ribonuclease A Ribonuclease A Ribonuclease A Ribonuclease A Ribonuclease A Ribonuclease A Ribonuclease A Ribonuclease A Ribonuclease A Ribose Saccharides Saccharin Salicylamide Salicylic acid Salicylic acid Sar Scopolamine Ser Page Figure 25 43 18 46 63 63 43 39 39 39 39 39 39 39 40 40 40 37 72 78 37 37 71 74 76 79 18 72 16 71 63 63 79 25 72 75 68 43 73 43 61 65 65 46 31 74 31 37 72 72 25 68 18 25 75 61 35 36 36 36 44 48 58 64 68 37 42 25 26 68 78 37 25 37 12 64 7 70 105 106 61 53 53 53 53 53 53 53 54 54 54 51 121 154 50 51 119 137 149 164 11 122 3 117 105 106 160 13 120 138 113 64 130 64 100 111 111 71 32 134 32 49 120 120 13 113 7 13 138 101 38 46 47 48 65 74 91 107 115 49 60 14 21 114 155 51 17 50 101 . Compound Ser Ser Ser Ser Ser SO42− SO42− SO42− SO42− Sorbic acid Sorbic acid Sorbic acid Sorbic acid Sorbitol Sorbitol Sorbitol Sphingomyelin Stevioside Stevioside Substance P Substance P Succinate Succinic acid Succinic acid Succinio acid Sucrose Sucrose Sucrose Sucrose Sucrose Sucrose Sucrose Tartarate Tartaric acid Tau Tau Taurine 1−Tetradecanol Theanine Theophyline Thiamine Thiamine Thiamine Thiamine Thiamine Thr Thr Thr Thr Thy Thy Thymidine Thymine Thymol Thyroglobulin Tocol α−Tocopherol α−Tocopherol α−Tocopherol α−Tocopherol β−Tocopherol β−Tocopherol β−Tocopherol β−Tocopherol γ−Tocopherol γ−Tocopherol γ−Tocopherol γ−Tocopherol δ−Tocopherol δ−Tocopherol δ−Tocopherol δ−Tocopherol Transferrin Transferrin 102 Page Figure 37 71 73 76 79 73 46 77 82 25 68 72 74 43 43 73 25 31 74 60 61 73 72 73 43 27 37 43 65 65 73 73 73 72 37 79 75 60 74 78 18 25 68 72 75 71 74 76 79 37 37 32 25 76 44 32 27 31 32 73 27 31 32 73 27 31 32 73 27 31 32 73 44 48 51 119 137 149 164 126 70 152 177 14 114 123 135 62 63 128 12 32 134 95 104 126 122 130 64 26 49 63 109 111 127 128 126 122 51 164 140 94 136 156 7 13 113 120 138 119 137 149 164 50 51 34 16 144 65 36 25 31 36 131 25 31 36 131 25 31 36 131 25 31 36 131 65 74 Compound Transferrin Trp Trp Trp Try Trypsin inhibitor Trypsin inhibitor Tyr Tyr Tyr Tyr Tyr ⊿Tyr UDP UDP UMP UMP Undine Unknown Uracil Uracil Urease Uric acid Uric acid Uridine UTP UTP Val Val Val Val Val Val Vanillylmanderic acid (VMA) Veleraldehyde Vitamin A Vitamin A acetate Vitamin A palmitate Vitamin B6 Vitamin B1 Vitamin B12 Vitamin B12 Vitamin B2 Vitamin D2 Vitamin D2 Vitamin D2 Vitamin D3 Vitamin E Vitamin E Vitamin E acetate Vitamin K2 VLA VMA Xanthine Xylose Xylose Xylose y−Globulin Zn2＋ Page Figure 58 71 76 79 76 36 58 37 37 71 74 79 71 35 78 35 78 32 25 25 72 35 64 78 25 35 78 37 37 71 73 76 79 79 18 71 26 26 61 61 61 77 61 26 60 71 60 26 71 26 26 51 51 78 27 37 43 44 46 90 119 149 164 149 43 90 50 51 119 137 164 119 42 158 42 158 34 16 16 124 40 108 157 16 42 158 50 51 119 137 149 164 161 11 118 20 20 99 99 99 151 99 23 98 118 98 20 118 20 20 81 81 157 26 49 63 65 73 . 2 . technical support and applications centers on six continents. 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