Proposal for the Release of Hybrid Maize Variety - 2011

Draft copy1 Proposal for the Release of “Rampur Hybrid-2” Developed from RML-4 and NML-2 Submitted by National Maize Research Program (NMRP), Rampur Proposed by DB Gurung§, SR Upadhyay§, TR Rijal§, CB Kunwar§, BR Pandey§, JB Kshetri§, DC Paudel§, BH Adhikary§, G KC§, P Thakur§, IP Upadhyay§, BB Pokharel§, J Shrestha§, B Bhandari§, N Dhakal§, GS Bhandari§, KP Dhital§, RK Rajak£, YP Sah₫, CL Chaudahry€, S Poudel†, N Dhami‡, KP Bhurer£ and MP Tripathi§ A Joint Accomplishment of: § National Maize Research Program (NMRP), Rampur Regional Agriculture Research Station (RARS), Parwanipur € Regional Agriculture Research Station (RARS), Nepalgunj ₫ Agriculture Research Station (ARS), Belachapi † Regional Agriculture Research Station (RARS), Tarahara ‡ Agriculture Botany Division (ABD), Khumaltar £ July, 2011 2 Application to be made for release of new maize varieties Government of Nepal Ministry of Agriculture and Cooperatives National Seed Board Format for submission of proposal for the release of “Rampur Hybrid-2” maize variety The Variety Approval, Release and Registration Sub-committee (VARRC) National Seed Board Secretariat Seed Quality Control Centre Harihar Bhawan, Lalitpur Tel. +977-01-5521359 Sub: Application for the release of maize variety “Rampur Hybrid-2” As, we have bred the seeds of new variety described below, we hereby make this application for the release of the seeds of the variety. Original entry name RML4/NML2 RML4 (CA00326) NML2 ( CML-430) Suggested name English Nepali Rampur Hybrid 2 Rampur Barnashankar -2 NML3 NML2 1. Background information In Nepal, maize is the second most important crop after rice in terms of area and production. It occupies a momentous position because of the multiple uses of this crop. It can be used as food, feed, fodder, fuel and raw materials for various industrial purposes. Maize is the main ingredient for poultry and animal feeds. The exponential growth of the human population and change of food habit in the recent years, people are consuming more milk, meat, fish and eggs because of increasing trends of income every year. The increased demand of those items needs more maize grains for poultry and animals feeds. Maize has low reserve in the international market compared to other cereal crops and has not a market problem worldwide because of the high demand of milk and meat products. Farmers in maize growing countries including Nepal have not problem of marketing of maize grains. In developed countries, maize is considered as an industrial crop whereas in Nepal, it is emerging as cash crop in the areas where green cobs are sold. In addition, Maize grain produced in many terai and in some mid hill districts can sell their maize immediately after harvesting and thus emerging maize as cash crop. The ever increasing demand of maize cannot be met easily simply cultivating with open pollinated improved varieties. The productivity of OPVs cannot be increased from a certain level even if provided with more fertilizers and improved crop management. The productivity of maize can be increased by growing with different types of hybrids particularly single cross hybrids. In the recent years, there is overwhelming demand of hybrid seeds and hybrid technology mostly in terai and inner 3 terai where hybrids are grown after rice in winter and spring seasons. The demand is also there in some mid hill districts and in valleys of foot hills and river basin areas. Of the 23 maize cultivars released in Nepal, Gaurav, single cross hybrid maize, which was released in 2003 in the history of hybrid maize breeding efforts initiated since 1987. This historical hybrid could not be popularized in hybrid maize growing areas because of the difficulty in its seed production particularly in private sector. This situation has left no varietal options for growing hybrid maize in Nepal and farmers have to rely on seeds of multi-national hybrid companies based mostly in India. In many cases, farmers are paying higher prices for hybrid seeds and in some cases; they are also cheated both in price and quality. The available options for the hybrid maize growing farmers are limited. Either they should grow improved OPVs developed by NMRP or go through the Indian hybrids sold by private seed entrepreneurs. They grow Indian hybrids simply because of unavailability of seeds of our Nepalese hybrids, higher yields of hybrids compared to OPVs, uniformity in maturity, and tolerance to biotic and abiotic stresses. To address this real situation, development of high yielding as well as competitive hybrids and releasing hybrids in a regular basis is the sustainable way of increasing production and productivity of maize in order to meet the ever increasing demand of maize for feeds in accessible areas and human consumption for mid hills. Releasing hybrid varieties in a cyclic order will create more varietal options and make breakthrough in the history of maize production in Nepal with hybrids and hybrid technology, which ultimately help to improve food security and reduce poverty and hunger in the country. Keeping all these in consideration, NMRP has thus prepared and submitted a proposal to the National Seed Board (NSB) for release a single cross maize hybrid with proposed name is Rampur hybrid-2 in English and Rampur Barnashankar -2 in Nepali. 2. General Information 2.1. Common name: Maize 2.2. Botanical name: Zea mays L 2.3. Original designation/Symbolic name used in testing: RML4/NML2 2.4. Pedigree: RML4 (CA00326)/NML2 (CML430) 2.5. Cross (parents): RML4/NML2 2.6. Country of Origin: Nepal 2.7. Source of material (farmer/community, name of the experiment and the year in which it was first introduced): CIMMYT 2.8. Years and location of testing of the variety in Nepal Table 1. Summary of the trials conducted through NMRP, Rampur in 2005-2011 Year 2005 2005 2006/07 2010W 2006/07 2007W 2007W 2010-11W 1 Experiments Inbred line Observation nursery Diallel crosses Observation nursery Agro-morphological characterization CVT CVT CVT Multi-location testing of hybrids Locations Khumaltar Rampur, Parwanipur Rampur Rampur Rampur Rampur Rampur Rampur, Parwanipur, Tarahara Please, find the details of experimental results referred as Annexure numbers at the end. References1 Annexure 1 Annexure 2- 3 Annexure 4- 5 Annexure 6 Annexure 7 Annexure 8 Annexure 9-10 Annexure 12 4 2009 2007 2008W 2009W 2007W 2008 2007w 2010S 2006S 2009S 2009 Front line demonstration Agronomic trials Agronomic trials Agronomic trials CFFT CFFT CFFT Heat stress screening nursery Disease screening nursery Disease screening nursery Insect screening nursery Rampur Rampur Rampur Rampur Rampur Rampur, Parwanipur, Belachapi Rampur Parwanipur, Rampur, Nepalganj Rampur Rampur Rampur Annexure 11 Annexure 20 Annexure 21-25 Annexure 26-28 Annexure 17 Annexure 18 Annexure 19 Annexure 13-16 Annexure 29 Annexure 30 Annexure 31-32 2.9. Farmers Field Trial (FFT), participatory trial data/information and stakeholders’ preferences Days to 50% silking ranged from 71 in early winter to 119 days in late winter in the farmers field condition (Table 2). The yield performance of this genotype is highest (8183 kg/ha) when planted in early winter. Table 2. Performance of RML4/NML2 under farmers condition (Annexure 17-19) Year Experiments Location 2007EW 2008LW CFFT CFFT 2007LW CFFT Rampur Rampur, Parwanipur, Belachapi Pithuwa Days to 50% silking 71 111 Plant height (cm) 210 133 Ear height (cm) 110 52 Grain yield (kg/ha) 8183 5677 119 170 95 5701 EW=Early winter, and LW= Late winter 2.10. Others, if any: Figure 1: Joint regression analysis of grain yield (kg ha -1) of RML4/NML2 maize hybrid over standatrd check (n=16) Rampur Composite is well known for its grain yield stability over the location and years among the maize growers. Probably, it is the most adaptive maize variety ever released by the National Maize Research Program (NMRP). Joint regression analysis of the proposed hybrids with 5 Rampur Composite (Figure 1 and Table 3) showed that RML4/NML2 is more stable (b=0.91 i.e, b= nearly unity) and mean grain yield 36.7% higher than standard check (Rampur Composite). Table 3. Joint regression analysis parameters of maize hybrids and Rampur Composite Genotypes RML4/NML2 Rampur Composite Mean±SEm 6393±530 4675±266 Range 2308-9255 3262-6307 b 0.9124 0.4810 SEb 0.2369* 0.1122* T value 3.85 4.29 3. Summary of Varietal Characteristics 3.1 Agronomic This is a full-season hybrid and has yield potential up to 10.5 tones/ha. Kernel is yellow and flint type. Plant stature is similar to Gaurav hybrid. Its erect leaves and medium plant height offers high density planting. Stem is robust and has no lodging problem. This hybrid can be grown in the winter, spring and summer seasons in terai and inner terai regions. However, main growing seasons are winter and summer seasons. It performs well in winter as compared to spring and summer seasons.The difference between days to tasseling and silking is 3-5 days but tasseling days ranged from 64 days in summer to 103 days in late winter. Silking days ranged from 67 in summer to 107 days in late winter (Table 4 and Annexure 3-15). The hybrid produced higher grain yield in early winter planting (7005 kg ha-1) followed by late winter (5579 kg ha-1) and summer (3555 kg ha-1). The major morphological traits of the inbred lines are presented in the Table 5. Table 4. Major agro-morphological traits of RML4/NML2 maize hybrids (Annexure 4-16) Parameters Days to 50% anthesis Days to 50% silking Plant height (cm) Ear height (cm) Grain yield (kg ha-1) Winter Early Late 66 (63-75)§ 103 (91-119) 71 (68-78) 107 (95-122) 203 (153-225) 126 (95-176) 113 (72-137) 53 (37-80) 7005 (5224-8694) 5579 (4395-7188) Summer 64 (58-68) 67 (59-71) 166 (153-181) 78 (68-91) 3555 (2308-4802) § Figures in the parenthesis shows the range Varietal characteristics of parental lines (Inbred lines) Seed parent (RML-4) is short and has cylindrical ears with irregular row arrangement and produces a good seed yield 1.5- 3 t ha-1. Pollen parent (NML-2) has medium plant height with erect leaves and glabrous leaf sheath. Pollen parent takes 69 GDU to shed pollen of seed parent when the seeds are planted at the same time. Parents are easy for crossing and varietal maintenance is not so difficult. Table 5. Agro-morphological traits of RML4 and NML2 inbred lines (Source: Annexure 6) Parameters Days to 50% Tasseling Days to 50% silking Plant height (cm) Ear height (cm) Grain yield (kg/ha) GDU for Tasseling GDU for Silking RML4 59 62 120 60 1250 953 967 NML2 71 75 190 80 1500 1036 1050 6 Fig 2. RML-4 x NML-2 in field at Fig 3. Ears of RML-4 x NML-2 NMRP, Rampur Fig 4. Seed Parent (RML-4) Fig 5. Pollen parent (NML-2) Fig 6. Ears of RML-4 3.2. Response to stresses (please specify): 3.2.1 Biotic stresses (a) Insects: Among the 32 tested genotypes including OPVs, QPM, Hybrids and Local check in Rampur. It is apparent from the results that RML4/NML2 showed minimum infestation of maize stem borer. The exits holes formed by the pests were also found minimum in this genotype (Annexure 31). (b) Diseases: Hybrid RML-4/NML-2 has shown resistant to moderately resistant reaction against leaf blight diseases (Northern leaf blight, Southern leaf blight and Banded leaf and sheath blight (BLSB) in natural condition (Annexure 29 and 30), though it’s inbred exhibited moderately susceptible reaction against NLB under artificially inoculated condition (Annexure 4 and 30). (c) Weeds: No specific association of weeds with this variety and does not require any special care for weed management. However, the field should be free from weed to attain higher grain yield and crop management practices applied to other maize hybrids or OPVs is also sufficient for this variety. 3.2.2 Abiotic stresses (a) Adverse climatic conditions: The hybrid maize lines along with other promising lines were evaluated under heat screening nursery at NMRP, Rampur; RARS, Parwanipur; and RARS, Tarahara in summer season of 2010 (Table ). The results showed that it prefer favorable environment rather than heat stress condition for higher yield performance. The crop could not perform well under any type of stress condition such as water stagnation, drought, heat and cold stresses. The genotypes can flourish its potentiality under optimum condition. 7 (b) Adverse soil conditions: The crop prefers loam to sandy loam soil for better yield performance. It requires frequent irrigation and heavy dose of manure and fertilizer than OPVs to obtain expected yield. (c ) Other stresses, if any: 3.3. Grain/leaf/root/curd/bulb/seed/fruit/tuber qualities: 3.3.1 Nutritional quality (if available): Table 6. Nutritional analysis of RML4/NML2 Traits Ash (%) Moisture (%) Fat (%) Protein (%) Crude fiber (%) Carbohydrates (%) Thousand grains weight (gm) Bulk density (gm/litre) Source: Food Research Unit, 2011 Value 1.29 14.73 3.06 7.59 2.02 71.31 287 816 3.3.2 Processing quality: 69% grit, 24.1% flour, 6.9% bran and 4:1 grit:flour ratio 3.3.3 Organoleptic test (cooking quality, taste, aroma/flavour, etc): % popping=15.5% (machine), 60% (earthen pot), 45% (metal pot) 3.3.4 Other specific qualities (if any): Stay Green characters 3.4. Other characteristics (threshing, storability, market potential etc.): 4. Morphological characteristics (size, shape, colour, etc of various plant parts): Table 7. Major morphological traits of proposed hybrids and its inbred lines Traits Stem color Plant height Leaf color mid rib color Leaf sheath Leaf orientation No. of leaves below ear No. of leaves above ear Tassel type Tassel color Tassel branch Silk color Grain color Grain type RML-4/NML-2 Green Medium Green White Green semi erect 8 5 Semi Open purple 11-12 pink Yellow Semi flint RML4 Green Medium Green White Green Semi erect NML 2 Green Tall Green White Green Semi erect Semi Open white Semi Open white white Yellow Semi dent Semi purple Yellow Flint 8 4.1. Major identifying characteristics of a crop variety for cultivar authenticity: The genotype can be distinguished from others maize genotypes by following traits. RML4/NML2: Kernel is yellow and flint type. Erect and stay green leaves, robust stem and medium plant height. RML4: Seed parent is short plant height, cylindrical ears with irregular row arrangement NML2: Pollen parent has medium plant height with erect leaves and glabrous leaf sheath 4.2. Molecular characteristics (Not available) 5. Recommendation domain: 5.1 Geographical area (altitude, latitude and longitude): The hybrid was tested at many locations from east to mid-western terai in the various experimental trials i.e. screening nursery, observation nursery, CVT and FFT (Table 1). Therefore, this variety is proposed to recommend for commercial cultivation from east terai to mid-western Terai, and inner Terai of Nepal. 5.2 Moisture regime: partially irrigated and irrigated. Soil moisture as well atmospheric moisture is critical at 7 days before and after flowering. 5.3 Climatic conditions: If the atmospheric temperature rises more than 38 oc pollen grains start to die by appearing tassel firing symptoms and atmospheric temperature below 8o C is not also suitable during flowering time. 5.4 Cropping pattern: The variety is suitable under both maize and rice based cropping system. However, higher level of production can be obtained when the crop is grown in winter rather than summer planting. a. Maize based cropping system: Maize-Soybean-Vegetables Maize-Rajma-Vegetables Maize-Sesame-Maize Maize-Black gram-tori etc. b. Rice based cropping system: Rice-Maize-Mungbean Rice-Maize-Cowpea Rice-Maize-Vegetables Rice-Wheat-Maize Maize-Maize-Rice etc. 5.5 Socioeconomic conditions: The farmers who are growing maize in terai and inner terai condition can easily grow this variety. However, this variety requires special care and attention than OPVs but similar level of management as given to Indian hybrids is required. Large scale seed production of inbred lines and hybrid seed production can be done by private sectors with qualified manpower and by an enthusiastic people who can bear the risk of handling large scale seed production and can get benefits from this technology. Since the variety has stay green character, farmers can use as fodder after harvesting and get more benefits from this variety than others from milk and milk products. So, the variety is friendly even to resource poor farmers, who can get higher yield per unit area. 9 5.6 Economic analysis and benefit: Hybrid seed production could be a very remunerative seed business in Nepal. Modern inbred lines give at least 3 t ha -1 of seeds. Seed parent covers about 75% of total area if we plant in a ratio of 3:1. If we assume only 80% pollination success, hybrid seed yield from 1 ha would be 3000 x 0.8 x 0.75 = 1800 kg of seeds. Retail price of different categories of hybrids in market is ranged from NRs 85- 500/kg. If we sell at whole sale rate of NRs. 150/kg, 1 ha will generate a total income of 1800 x 150 = NRs. 270, 000. Cost involved in seed production depends on the localities where the seed production is done. Based on the rough estimation, Rs.100, 000/ha would be sufficient to cover all the production costs. Thus, net profit would be Rs. 170,000 per ha. This clearly shows that hybrid maize seed production is a highly profitable business. Private seed companies should come forward to commercialize the hybrid seed production as their role is crucial in commercialization of hybrid seed and hybrid technology. 6 Production and management aspect (Please add detailed information, if necessary, on the general cultivation practices; appropriate input and moisture regimes): Package of practice is similar to the Gaurav, released in 2003 and other hybrids of multi-national companies, which are growing by farmers in hybrid maize growing districts. 6.1 Agronomic Practices:  2-3 ploughings are needed for land preparation. First ploughing should be carried out 30 days prior to maize sowing.  The appropriate date of maize sowing in Terai and inner Terai of Nepal ranges from 15th August to15th October, respectively. However, it can be grown before 15th November.  The recommended dose of fertilizer 160:60:40 N:P2O5:K2O kg ha-1 along with 10-15 tons of farm yard manure. However, it gives highest grain yield upto 180 K2O kg ha-1 nitrogen (Annexure 28). FYM and full dose of P205 and K20 along with 50% nitrogen should be applied during last ploughing (before crop sowing). 25% Urea should be top-dressed during knee high stage and 25% just prior to tasseling and silking.  Seed rate 25 kg ha-1 (66666 plants/ha) for general grain production.  The recommended planting space for hybrid variety is given in (Annexure 21, Annexure 23 and Annexure 24): Row-to-Row distance: 75 cm Plant to plant distance: 20 cm 6.1.1 Weeding: First weeding should be done within 20-25 days and second weeding (earthing up) within 40-45 days after the germination. Priority should be given to manual weeding. However, Glyphosate @ 5ml/litre as pre-planting and Pendimethylene @ 5.5 ml/litre can be applied as pre-emergence. Post emergence can be applied with 2-4-D @ 3ml/litre of water. 6.1.2 Irrigation: Knee high stage, tasseling and silking, and grain filling stages are most critical for moisture stress. Light irrigation should be applied to the maize crop at these stages depending on the soil moisture status and irrigation facilities. 6.1.3 Detasseling: If weather condition is normal (high humidity and temperature less than 38oc), 30% plants can be detasseled, which helps to increase maize production up to 7%. 10 6.1.4 Harvesting: Crop should be harvested after physiological maturity. Maize can be harvested when black layer is formed on maize grains. 6.1.5 Drying: Maize ears should be sun dried for 3-4 days and before shelling. Grains should be dried on the tarpaulin and covered during the night. When the grain moisture is reduced down to 12%, then grains are either stored in metal bins or kept in sealed bags after processing of seeds. 7. Reasons for release  There are very limited hybrid varietal options for growing hybrids in Nepal. A single cross hybrid “Gaurav “ was released in 2003 but it could not be popularized among the farmers due to difficulty in its seed production particularly in private sector. NMRP could not release hybrids in a regular basis due to low investments of resources in hybrid breeding program. Due to unavailability of hybrid seeds within the country, farmers mostly in terai, inner terai and in road accessible areas of foot hills started to buy Indian multi- national hybrid seeds due to high yield potentiality and uniformity of hybrids over OPVs. However, farmers in those areas had been growing maize hybrids since many years. Nowadays, there is overwhelming demand of hybrid seeds due to high demand of maize grains for poultry, animal feeds and other industrial purposes.  Farmers in hybrid maize growing areas in Terai and in some mid hill districts are using hybrid seeds mostly from multi-national seed companies based mostly in India and seeds are expensive ranging from NRs, 85 to 500/kg. Huge amount of money is spent to purchase maize seeds from India. This investment can be minimized and outgoing money can be minimized and saved. Seeds will be cheaper as we produced within the country and quality is assured and there is no chance cheating farmers in the name of fake hybrid seeds.  Releasing this variety creates a better opportunity for commercialization in hybrid maize seed production in private sector and could benefit the farmers, seed growers and others, who are involved in seed production and distribution chains. Country will be benefit in the long run with improved food security through increased production.  This variety is moderately resistant to resistant against foliar diseases especially Northern leaf blight, Southern leaf blight, and Banded leaf and sheath blight. It gives stable and higher grain yields up to 8.6 t ha-1, uniformity in plant, ear height and in maturity.  As it creates more varietal options and farmers will have choices for growing maize hybrids. Dependency in importing maize grains required for poultry and animal industries will be reduced and steps towards self-sufficiency of maize in the country will be kicked off. 8. Seed supply situation NMRP has been maintaining numbers of inbred lines and many released and pre –released maize varieties since many years. Although the available seed of these inbred lines and hybrids are not 11 sufficient for selling in the market but it can provide sufficient amount of source seed for seed multiplication purpose as demanded by the interested stakeholders. Table 8. Seed supply situation of inbred lines and hybrids at NMRP, Rampur SN 1 2 3 Category of seed RML4 NML2 RML4/NML2 Amount available (kg) 5 3 20 Where available NMRP, Rampur NMRP, Rampur NMRP, Rampur Person handling B Bhandari B Bhandari B Bhandari 9. Acknowledgements The present work is an outcome of a team and collaborative work in varietal development, which is a part of regular research activities of National Maize Research Program (NMRP), Rampur. We are deeply indebted to the NMRP staffs and collaborators, farmers and others who are directly or indirectly involved in the development and evaluation phases of this hybrid. We are thankful to CIMMYT for providing inbred lines during the initial stage and other NARC collaborating stations in providing support in evaluation process. Hybrid maize breeding team is grateful to the NARC management for over all support in hybrid maize breeding program. 10. References NMRP, 2007. Proceedings of the 25th National Summer Crops Research Workshop on Maize Research and Production in Nepal, June 21-23. NMRP, 2008. Annual Report 2063/64 (2006/07). National Maize Research Program (NMRP), Rampur NMRP, 2009. Annual Report 2064/65 (2007/08). National Maize Research Program (NMRP), Rampur, Chitwan, Nepal NMRP, 2010. Annual Report 2065/66 (2008/09). National Maize Research Program (NMRP), Rampur, Chitwan, Nepal NMRP, 2011. Annual Report 2066/67(2009/10). National Maize Research Program (NMRP), Rampur 12 Proposed by Signature: Name: Dr. Dil Bahadur Gurung Main proposing organization: National Maize Research Program (NMRP), Rampur Designation: National Maize Coordinator /Plant Breeder Corresponding address: National Maize Research Program (NMRP), Rampur, Chitwan Tel. +977-56-591001 Fax: +977-56-592548 Mobile: 9851074849 Email: [email protected] Website if applicable: 13 Annexes Annexure 1: Inbred lines Observation Nursery, Khumaltar in 2005 SN Inbred lines 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 RML-59 RML-37 RML-17 RML-54 RL-57 RML-3 RL-41 RL-31 RML-8 RML-5 RL-35 RML-18 NML-1 RML-24 RL-1 RML-51 RML-4 RML-57 NML-2 RL-30 RML-6 Mean Plant height (cm) 177 176 194 211 174 208 164 180 142 170 160 155 190 178 160 135 129 137 182 157 140 168 Ear height (cm) 95 81 102 106 77 118 74 98 67 76 66 61 90 79 81 67 62 71 101 66 73 81 Turcicum Leaf Blight (TLB) 2 2 1.5 2 1.5 1.5 2 2 2 2 1.5 1.5 1 2 2.5 1.5 2.5 1.5 2 1.5 2.5 1.8 Husk cover Ear Aspect 3.5 1.2 1 1.8 1 1 1 1 1.2 1 1 1 1.2 1 1.2 1 1.2 1 1 1 1.2 1.2 1 1.3 2 1.5 1.8 1.5 1.3 1.6 2.8 1.2 2 2.8 2.2 1.6 2.8 1.5 2.5 3 2.5 2.8 2 2 Grain yield (kg ha-1) 6840 4869 4213 3795 3779 3515 3453 3109 2573 2504 2389 2235 1867 1840 1699 1491 1427 1360 1203 1173 1115 2688 Annexure 2: Mean grain yield (kg ha-1) over locations of F1 hybrids Parents NML1 NML2 RML3 RML4 RML5 RML6 RML7 RML8 Grand mean NML2 7277 RML3 8693 6705 RML4 7240 7861 8409 RML5 9673 8728 7529 7901 RML6 7136 9058 8230 6326 7625 RML7 5361 6786 7772 8519 7465 8309 RML8 7570 6848 8646 7698 8139 8011 7401 xi. 52950 53263 55984 53954 57060 54695 51613 54313 Mean 7564 7609 7998 7708 8151 7814 7373 7759 7747 Source: Proceedings of the 25th National Summer Crops Research Workshop on Maize Research and Production in Nepal, June 21-23, 2007 Annexure 3: Mean grain yield of 28 Diallel crosses (Hybrids) from 8 maize inbred lines and checks at Rampur and Parwanipur in winter season of 2005 SN 1 2 3 4 5 6 7 8 Cross NML2/NML1 RML3/NML1 NML1/RML4 RML5/NML1 RML6/NML1 RML7/NML1 RML8/NML1 NML2/RML3 Rampur 6719 8582 7557 9321 7939 5357 7351 5577 Mean grain yield (kg ha-1) Parwanipur 7835 8803 6923 10025 6334 5366 7789 7832 Combined 7277 8693 7240 9673 7136 5361 7570 6705 14 9 RML4/NML2 7890 7831 7861 10 NML2/RML5 8614 8842 8728 11 NML2/RML6 9327 8789 9058 12 NML2/RML7 7197 6376 6786 13 NML2/RML8 6633 7064 6848 14 RML4/CRML3 8792 8025 8409 15 RML30/RML5 7952 7105 7529 16 RML3/RML6 8306 8154 8230 17 RML3/RML7 6476 9068 7772 18 RML3/RML8 8914 8378 8646 19 RML4/RML5 7855 7946 7901 20 RML4/RML6 5239 7413 6326 21 RML4/RML7 9118 7919 8519 22 RML4/RML8 8812 6584 7698 23 RML5/RML6 7839 7411 7625 24 RML5/RML7 6942 7988 7465 25 RML5/RML8 8317 7961 8139 26 RML6/RML7 9392 7226 8309 27 RML6/RML8 8200 7822 8011 28 RML7/RML8 6915 7886 7401 29 JKMH1001 5824 6604 6214 30 GAURAV 6235 9530 7883 Grand mean 7640 7761 7700 Location (L) NS Genotypes (G) ** LxG NS CV, % 16.6 SE (Loc) 281.1 SE (G) 639 SE (L x G) 904 Source: Proceedings of the 25th National Summer Crops Research Workshop on Maize Research and Production in Nepal, June 21-23, 2007 Annexure 4: Mean grain yield and other yield attributing characters of maize hybrids in observation nursery at NMRP, Rampur in 2006/07 Cross RML4/RML3 RML73/RML72 NML2/RL12 NML1/RL17 RML52/RML51 RL17/P145-16-1-BBBB RC/RL189 RL30/RML6 RML3/NML2 RML54/RL51 RL14/RL35 GAURAV RML5/NML2 RML51/RML53 RL17/ARUN2 RML17/ARUN2 RC/RML4 RC/RML17 NML2/RL17 RC/NML1 50 % anthesis 50 % silking Plant height (cm) Ear height (cm) 89 104 100 93 93 93 83 93 94 86 102 99 99 85 85 91 86 90 105 84 94 106 107 102 100 97 86 97 102 93 104 104 104 93 87 93 88 93 104 86 118 143 127 135 137 118 143 117 133 140 118 128 127 140 137 167 105 140 122 130 67 58 58 62 70 53 68 48 68 57 53 58 53 68 57 83 48 58 53 60 Grain yield (kg ha-1) 9077 8189 7848 7592 7087 6912 6805 6701 6588 6586 6578 6508 6483 6461 6453 6393 6367 6334 6324 6322 Plant aspect (1-5) Ear aspect (1-5) Helminthosporium leaf blight (HLB) 1.5 1 1.5 2 1.5 2 2 1.5 1.5 1.5 1.5 1.5 1.5 2.5 1.5 2 2 1.5 1.5 2 1.5 1.5 2.5 1.5 2 3 2 1.5 2 1.5 3 2 2.5 2 1.5 1.5 2 2.5 2 2 8.7 18.3 11.7 18.3 12.3 26.7 10 15 9.3 15 14.3 23.3 25 18.3 25 28.3 31.7 13.3 15 25 15 RML4/ARUN2 RC/RML6 RML7/RML8 RL49/RL85 NML1/ARUN2 PIONEER30G10 RML4/NML2 RC/NML3 RL30/RL35 RML6/NML2 RC/NML18 RL7/RL90 RL19/RL177 RC/RML5 RML18/RML17 RL7/RL94 RL19/RL161 RL19/RL166 NML1/RML7 RL14/RML8 RML18/ARUN2 RL14/MUSTANG RC/RML7 RL21/RL35 RL19/RL167 RC/RL149 RC/RML10 RML8/MUSTANG RC/ARUN2 RML4/MUSTANG RC/RL192 RC/RML8 RL30/MUSTANG RC RL35/MUSTANG RL35/RML8 NML2/MUSTANG RL19/RL133 RML6/MUSTANG RML5/MUSTANG NML1/MUSTANG RC/MUSTANG RML18/MUSTANG ARUN2/MUSTANG Grand mean F test CV, % LSD0.05 85 83 86 81 84 86 99 76 89 96 83 87 81 77 99 86 83 85 89 88 81 66 84 100 85 81 84 65 68 66 74 83 64 76 64 93 68 81 64 68 63 64 65 63 88 87 97 81 85 88 101 82 91 98 87 91 85 82 98 93 85 87 97 97 84 68 79 105 88 86 87 68 89 68 82 85 64 83 66 98 69 84 65 69 66 67 68 66 117 155 138 157 117 128 132 140 117 138 148 115 173 138 110 117 150 165 108 127 128 110 138 108 139 148 120 127 140 93 152 145 114 125 118 125 99 128 106 102 106 128 105 118 129 ** 11.57 21.07 47 60 63 70 50 48 65 65 52 55 62 45 80 67 38 52 62 78 43 53 53 37 67 52 53 53 58 41 55 30 67 60 44 50 46 60 47 50 29 28 34 48 33 45 55 ** 19.18 16.96 6049 6014 5906 5849 5801 5725 5672 5646 5611 5579 5466 5454 5383 5260 5192 5161 5147 5085 5014 4894 4869 4852 4823 4798 4795 4696 4508 3913 3887 3853 3817 3678 3657 3650 3611 3529 3452 3431 3315 3210 3031 2857 2243 2065 5282 ** 20.78 1773 2 1.5 1 1.5 2 1.5 1.5 1.5 1.5 1.5 2 1.5 2 1 1.5 2 1.5 2 2 1 2.5 2 2 2.5 2 2 1.5 2 2 2 1.5 1.5 1.5 2 1.5 1.5 2 2.5 2.5 2.5 2 2 3 2 1 2 2 2 1 2 1.5 2.5 2 1.5 2 2 2 2 2 2 3 2.5 2 2.5 1.5 2 3 3 2 3.5 2.5 2.5 2 2 4 3 2 3 2 2.5 2 4 2.5 2 2 2 2.5 2 38.3 16.7 26.7 43.3 23.3 23.3 18.3 13.3 26.7 13.3 31.7 48.3 33.3 18.3 25 43.3 26.7 25 31.7 11 38.3 36.7 43.3 35 26.7 35 33.3 41.7 46.7 40 45 21.7 40 41.7 41.7 25 45 41.7 41.7 33.3 48.3 38.3 35 46.7 Notes: RL=Rampur lines, RC= Rampur composite, CV=Coefficient of variation Source: Annual Report 2063/64 (2006/07). National Maize Research Program (NMRP), Rampur 16 Annexure 5: Mean performance of maize hybrids evaluated on Hybrid Observation Nursery (OBN) in 2067 winter at NMRP, Rampur Trt Genotypes Male Flowering Days Female Flowering days Plant Height (cm) Ear height (cm) No of plant/plot No. of ear/plot Rotten ears Grain yield (kg/10m2) Husk covers scores Plant aspects score Ear aspects scores Insect 1 10 13 14 16 17 18 19 20 21 30 32 33 35 36 37 38 39 40 RML-4/NML-2 RML-55/RML-40 RL-99/RL-161 RML-40/RL-168 RL-106/RL-161 L-4/L-2 PUTU-17/L-1 PUTU-17/AG-27 ARUN-2/RML-8 PUTU-13/L-20 L-19/L-20 RML-4/RML-17 PUTU-20/AG-27 RL-182/RL-174 RC/RML-8 RL-105/RL-197 RAJKUMAR PIONEER 30V92 PIONEER 30B07 103 103 100 101 102 103 105 104 95 102 106 111 102 102 97 108 97 101 100 107 107 103 105 105 107 106 107 98 105 109 114 103 106 100 112 99 105 103 95 98 87 97 70 100 90 95 95 102 80 93 100 113 108 120 108 95 92 37 37 27 37 23 27 35 37 37 42 29 30 29 39 40 43 40 34 35 10.0 3.5 3.0 4.5 10.5 6.5 3.0 8.5 8.0 8.0 3.0 11.0 10.5 8.5 6.5 5.0 11.5 10.5 12.5 11.0 5.7 5.7 4.3 9.3 9.0 3.7 12.7 8.3 9.3 4.0 12.3 12.7 7.0 7.7 6.3 9.0 9.0 13.7 0.0 0.3 0.3 0.0 0.3 0.3 2.7 0.0 0.3 0.0 0.5 0.0 0.0 0.3 0.0 0.0 0.0 1.7 0.0 5.692 3.357 1.144 1.200 3.735 4.039 1.350 3.077 2.565 4.281 1.384 5.101 6.081 1.877 3.887 2.402 4.984 4.612 7.961 1.7 2.0 2.7 2.2 2.0 1.8 1.7 1.7 2.0 2.2 2.0 1.5 1.8 1.7 2.0 1.7 2.0 2.5 1.7 1.5 2.3 2.7 2.3 2.8 2.2 2.2 1.7 3.0 1.2 1.7 1.5 1.8 1.8 2.7 1.7 2.0 2.0 1.5 1.7 2.3 2.5 2.2 2.5 2.3 1.8 1.8 2.5 1.8 1.8 1.5 1.8 1.8 2.7 1.7 2.0 1.8 1.5 0.3 0.3 1.3 0.3 0.7 0.3 0.3 0.3 1.7 0.3 0.7 0.3 0.3 0.0 1.3 0.3 2.0 2.3 0.7 41 Rampur composite Mean F test LSD0.05 94 102 ** 5.32 97 105 ** 6.09 112 98 * 23.53 45 35 NS 17.30 10.0 7.7 ** 2.55 13.0 8.7 ** 4.17 0.0 0.3 NS 1.87 3.938 3.633 ** 2.56 2.2 1.9 NS 0.69 1.8 2.0 ** 0.69 2.3 2.0 ** 0.56 0.7 0.7 NS 1.54 CV, % 3.2 3.5 14.6 29.9 19.4 29.1 332.5 42.7 21.7 20.9 17.0 127.2 17 Annexure 6: Agro-morphological characterization of 62 maize inbred lines in winter, 2010 at NMRP, Rampur SN Name of inbred lines Days Days ASI to 50 to 50% % silking tasselin g No. of leaf Leaf Plant Plant Ear Ear Tassel Tassel orientati height Aspec Aspect position structu branch on (cm) t (score (cm) re Number (score (score 1-5) (score (No.) 1-3) 1-5) 1-3) 1 RML-19 72 76 4 12.33 3 149.5 2 2 2 NML-2 71 75 4 12.33 2 191.5 1.5 1.5 3 RL-137 54 58 4 10.33 3 148.5 2 4 RML-4 59 62 3 11 1 120 5 RML-32 58 61 3 11.33 1 135.5 6 RML-17 74 77 3 12.33 2 7 RML-55 53 57 4 11.66 3 8 RL-29 53 55 2 11.33 9 RL-99 58 61 3 10 RL-72 57 62 11 RML-18 48 12 RML-6 13 Tassel Length (cm) Tassel Silk color color (score (score 1-3) 1-3) 45.5 3 8.66 42.66 1 1 80 3 10.33 38 3 2 2 62.5 3 14 29.33 3 3 1.5 2 55 2 7 35.33 3 3 1.5 1.5 70.5 2 13.66 38.66 1 2 162 3 2.5 67.5 2 9.66 33.33 3 2 172 2.5 2.5 105.5 3 18 36.33 3 3 3 147.5 1.5 2 82 3 13.33 28.66 3 3 11 1 140.5 2.5 2.5 61 2 10.66 32 3 3 5 10 3 99.5 2.5 2.5 60 2 5.33 38 3 2 54 6 12.66 1 185 1.5 1.5 90.5 2 17 42 3 2 71 75 4 12.33 3 165.5 1.5 2 65 3 8 40.66 3 1 RL-111 62 67 5 12.33 1 175 1.5 2 85.5 1 7 39 3 2 14 RL-189 71 74 3 13.33 3 203.5 2.5 2 100 2 13.66 45.33 3 2 15 RL-195 54 58 4 12 2 150 2.5 2.5 95.5 2 19.66 30.33 3 2 16 PUTU-17 58 62 4 11.66 1 131.5 2 2 62.5 2 13.33 41.66 3 2 17 PUTU-20 59 62 3 10.66 3 115 1.5 2 45 1 8.66 38.66 3 2 18 AG-27 62 66 4 12 3 170 3 3 75 3 11.66 42 3 2 19 RL-182 70 73 3 11.33 3 100 3 2.5 50.5 3 8 37.33 3 2 20 RL-174 70 74 4 10.66 1 140 2 2 55 3 10.66 35.33 2 2 21 RL-36 70 74 4 9.66 3 101.5 3 2.5 30 3 7.33 37.66 3 1 22 RML-36 68 72 4 10 1 99 2 2 50 2 7 36.33 2 2 23 RL-197 69 72 3 10 1 149.5 2.5 2.5 70 2 9 40.66 2 2 24 RL-180 53 58 5 12.33 3 211 3 2.5 91.5 3 16 43.33 3 2 25 RML-55 58 62 4 12.33 1 120 1.5 2 42 2 10.33 31 3 2 26 RL-5 59 61 2 12.33 1 150 1.5 2 75 2 14.66 32.33 3 1 27 RML-57 62 67 5 10 3 100 2.5 2.5 30.5 3 6 34.66 3 2 28 RML-6 62 66 4 12.66 3 107.5 1.5 2 25 3 8 36.66 3 2 29 RL-25 49 54 5 11.66 1 180 1.5 2 85.5 2 8.33 42.33 3 2 30 RL-170 61 55 -6 11.33 3 191 2 2 100 3 7 40.33 3 2 31 RL-154 61 66 5 10.66 1 143 1.5 2 60 3 10.33 34.66 3 1 32 RL-96 60 63 3 9.33 1 137 3 2.5 67.5 2 10 39.33 3 2 33 RL-102 62 65 3 9 1 100 2.5 2.5 45.5 2 6.66 36.33 3 2 34 RL-114 71 75 4 11.33 1 150 1.5 1.5 70 3 12 39.33 3 2 35 RL-153 61 65 4 11 1 140 1.5 2 66 2 9.33 35.33 3 2 36 RL-186 61 64 3 11.66 2 160 1.5 2 65.5 3 16 32.33 2 1 37 RL-150 62 66 4 11 1 136.5 3 2.5 55.5 3 14.33 31.66 3 2 38 RL-151 54 57 3 10.33 1 150 2 2 70 3 17.66 35.66 3 2 39 RL-86 57 61 4 11.33 3 160 1.5 2 80.5 3 4.33 42.66 2 2 40 RL-183 61 65 4 9 1 120 3 2.5 35 2 7 35.66 2 3 41 L-20 49 53 4 10.66 1 160.5 1.5 1.5 91.5 3 9.33 35 1 3 42 PUTU-22 61 64 3 10.33 3 140 1.5 2 55 3 9.66 36.33 3 1 43 PUTU-12 60 64 4 12 2 120 1.5 1.5 50 2 9 34 3 3 44 RL-85 49 53 4 11 1 152 1.5 1.5 60 3 14 46 3 1 45 L-10 71 76 5 11.33 3 95.5 2.5 2.5 35.5 3 6 30.33 2 1 46 L-3 59 62 3 12.33 2 110 1.5 2 55 2 7.33 37.33 3 3 18 47 PUTU-21 69 72 3 10.66 2 122 1.5 2 52.5 2 13.33 37.66 3 3 48 RML-62 72 74 2 12.33 3 150 2 2 111 3 11.66 35.33 3 2 49 RL-105 56 50 -6 10 3 170 2 2 85 2 10 38 3 2 50 RML-8 47 50 3 10.66 3 166.5 1.5 1.5 80.5 3 11.33 45.66 3 2 51 RL-165 58 62 4 10.66 3 140 2.5 2.5 65 3 9.66 40.33 2 3 52 RL-94 62 66 4 10.33 3 150 2 2 75 2 18 40.66 1 1 53 RL-17 52 56 4 10 2 190 2 2 90 2 6.33 30.33 1 2 54 RL-19 53 56 3 10.66 3 140 3 2.5 50 3 10.33 44 3 3 55 RL-106 58 62 4 9.33 3 110.5 2.5 2.5 43.5 3 6.33 37.66 3 1 56 RL-84 54 57 3 10.66 3 165 2.5 2 70 3 7.33 41.66 1 1 57 RML-55 58 62 4 8.66 3 161.5 3 3 75.5 3 6.66 34 3 1 58 L-17 55 64 9 8.33 3 95 3 2.5 45 3 7.66 27.66 3 3 59 RL-194 58 62 4 10.33 3 120 2.5 3 60 3 9 33.66 1 3 60 RML-62 64 68 4 10.66 3 170 1.5 2 85 3 8.33 37 3 3 61 RC 68 72 4 11 2 169.5 1.5 1.5 90.5 2 9.33 45 1 1 62 Arun-2 64 68 4 10.66 2 154 2 2 80 3 9.33 42.33 1 1 Annexure 7: Mean performance of single cross maize hybrids in CVT conducted at NMRP, Rampur in winter 2006/07 Cross RML4/NML2 RML4/RML5 RML4/RML8 RML8/RL30-1 NML1/RML8 RL12/RML8 RML6/RML8 NML1/RML6 RML18/RL17 RL17/RL30-1 RL29/RL30-1 RML41/RL30-1 GAURAV BIOSEED 9681 PIONEER 30G10 RAMPUR COMPOSITE Grand mean F test CV, % LSD0.05 Days to 50% anthesis 67 68 64 64 66 62 66 67 69 66 61 61 64 61 64 57 64 ** 3.5 3.8 Days to 50% silking 68 71 67 68 72 66 71 74 74 70 64 66 70 65 70 62 69 ** 3.8 4.4 Plant height (cm) 153 142 142 147 157 156 167 148 142 132 153 157 155 167 142 155 151 NS 10 Ear height (cm) 72 67 73 75 77 73 78 65 57 57 73 78 78 77 68 82 72 NS 14 Grain yield (kg ha-1) 7639 6567 6553 6043 7057 5671 7471 5265 6731 6641 5359 5803 6136 4091 5069 5497 6097 * 17.4 1841 Source: Annual Report 2063/64 (2006/07). National Maize Research Program (NMRP), Rampur 19 Annexure 8: Grain yield and other important traits of hybrids evaluated in CVT at Rampur in winter 2007 SN Genotypes 1 RML4/NML2 2 RML3/NML2 3 NML1/RML8 4 NML1/RML17 5 RL125/RL169 6 RML18/RML17 7 RC/NML1 8 RC/RML4 9 RC/RML17 10 RC/RML5 11 RL30-1/RC 12 RML4/ARUN2 13 GAURAV 14 PIO30G10 15 BIOSEED9681 16 Rampur composite Grand mean F test LSD0.05 CV, % Days to 50% anthesis Days to 50% silking Plant height (cm) Ear height (cm) Plant aspect (1-5) TLB (1-5) HC (1-5) 75 77 72 71 67 80 68 68 76 68 68 70 75 79 67 66 72 ** 5.92 5.0 78 80 75 74 70 83 71 71 80 71 71 73 78 83 70 69 75 ** 5.84 4.7 172 223 200 203 212 202 247 195 208 207 202 182 198 205 190 200 203 NS 42.91 12.7 98 113 97 103 105 95 113 113 102 103 107 92 113 103 82 97 102 NS 25.91 15.2 1.5 2.0 2.0 2.5 2.2 1.7 1.8 1.7 1.5 1.5 2.2 2.2 2.3 2.5 2.5 2.0 2.0 ** 0.39 11.8 1.8 2.0 2.0 2.3 1.7 2.2 2.2 2.0 2.2 2.2 2.2 2.0 2.2 2.2 2.2 2.5 2.1 NS 0.53 15.1 1.7 1.5 2.0 2.2 2.3 2.3 1.8 1.7 1.8 2.2 2.0 2.2 2.0 1.8 2.5 2.2 2.0 NS 0.67 19.9 Grain yield (kg ha1 ) 8694 6774 6863 7551 5838 8548 7461 8097 6518 6318 6002 7388 8577 6630 4169 6257 6982 ** 1320 11.3 RC=Rampur composite Source: Annual Report 2064/65 (2007/08). National Maize Research Program (NMRP), Rampur, Chitwan, Nepal Annexure 9: Grain yield and other traits of Indian and NMRP developed hybrids evaluated at Rampur in winter 2007 SN Genotypes Days to 50% anthesis Days to 50% silking Plant height (cm) Ear height (cm) Plant aspect (1-5) Ear aspect (1-5) Husk cover 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 ADITYA BADSHAH BIOSEED 9681 KESHAR KINGS SHUBHAM2 SOW101 RAJKUMAR PIONEER HYBRID NML1/RML8 NML1/RML6 PIONEER/RML8 NML1/RML5 NML4/NML2 RML3/NML2 GAURAV Rampur composite 98 97 96 95 84 99 93 93.5 102 102 94 106 105 107 104 91 101 100 100 98 87 102 97 97 109 106 97 110 108 110 107 94 123 128 130 105 133 118 140 145 115 140 115 103 130 123 110 120 58 45 50 45 43 43 55 63 43 53 43 38 60 48 45 45 2.5 2.8 2.8 2.8 2.5 2.3 1.5 1.5 1.3 1.5 1.8 1.3 1.5 1.8 1.5 2.3 2.0 2.3 1.8 2.5 2.8 2.8 1.5 1.8 1.3 1.3 2.0 1.3 1.3 2.0 1.3 2.5 3.0 2.5 2.8 2.0 2.0 2.3 1.5 1.3 1.5 2.3 1.0 2.0 1.8 1.3 1.3 1.3 Source: Annual Report 2064/65 (2007/08). National Maize Research Program (NMRP), Rampur, Chitwan, Nepal Grain yield (kg ha1 ) 6011 5465 4100 4099 4805 5348 5136 7269 6052 7195 6011 6997 7040 5379 7620 3785 20 Annexure 10: Mean performance of hybrid maize genotypes on different traits evaluated in Coordinated Varietal Trial (CVT) at NMRP, Rampur in 2010 EN Genotypes 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 RML-4/NML-1 RL-111/RL-105 RC/RML-8 RL-111/RL-197 CML-169/CML-167 RML-4/NML-2 CML-168/CML-187 GRGTS1-7-1-3CML-312/CML-172 1/RL-84 ARUN-2-S1-10-1-2ARUN-2 1/RL-84 S1-10-1-2RL-105/RL-197 2/RL-84 RC-S1-9-4-2-1/RLRC-S1-9-4-3-1/RL84 RC-S1-2-2-5-2/RL84 RC-S1-4-1-5-2/RL84 RC-S1-9-4-4-2/RL84 CLQ-6602/CML-172 84 CML-493/CML-172 CML-168/CML-167 CML-205/CML-172 CLRCYQ-07/CMLCML-493/CML-167 172 PIONEER-S1-5-2-1PIONEER-S1-9-2-42/RL-84 PIONEER-S1-5-3-31/RL-84 GAURAV/P45-9 2/RL-84 PIONEER-S1-1-1-2RAJKUMAR 2/RL-84 30V92 30B07 Rampur composite Mean F test LSD0.05 CV, % M a l e f 1 l 1 0 o 9 0 7 1 0 w 4 1 0 e 1 0 5 r 1 3 0 i 1 0 6 n 1 0 g 9 0 d 9 3 1 a 1 4 y 9 0 s 9 3 9 9 1 9 1 2 1 0 1 0 3 1 0 1 0 9 0 1 9 6 2 9 5 9 7 1 3 9 0 9 7 5 1 8 1 0 9 0 9 1 4 * 9 5 * 0 . .2 9 4 Fem ale flow ering days 110 108 94 108 106 110 103 103 105 97 97 107 96 102 95 103 94 107 105 104 105 106 99 99 100 97 109 101 101 104 108 95 102 ** 5.22 0.7 Anthesis silking interval (ASI) 3.7 3.7 4.0 3.3 2.3 4.0 3.7 3.3 3.7 3.7 3.0 3.7 3.0 3.7 3.7 4.0 2.0 3.7 4.3 3.3 3.7 3.7 3.3 4.7 3.7 3.3 4.0 3.7 3.3 4.0 4.0 4.0 3.59 NS 1.17 6.1 Pla nt hei ght (c 90 m) 11 10 0 11 8 87 0 98 10 10 8 11 0 10 3 11 3 14 5 83 0 85 12 10 0 10 8 11 7 88 0 10 10 8 10 0 10 8 10 7 95 7 88 95 83 11 10 2 95 7 92 10 * 3 22. 7.1 1 Ear hei ght (c m) 35 38 47 42 32 38 52 38 42 43 49 52 28 30 53 38 40 38 25 40 33 40 40 43 33 30 32 28 42 40 37 35 39 NS 16. 10. 82 4 No. of plant /plot 11 11 10 12 11 13 11 11 12 12 11 11 11 12 11 9 11 11 10 11 12 12 10 10 11 11 11 10 11 11 11 11 11 NS 2.18 2.0 No. of ear/ plot 10 12 11 12 12 11 12 13 15 15 16 15 14 13 11 10 10 15 10 10 14 11 12 12 11 11 16 11 14 13 12 10 12 * 3.3 5.0 8 Rot ten ear s 1 0 1 0 2 0 3 1 1 1 1 3 2 0 0 0 1 2 0 1 0 1 2 1 1 0 1 0 1 1 0 1 1 * 1.4 28. 9 1 Grain yield (kg ha1 ) 4663 5190 4025 2758 4299 4607 3181 4009 6439 4416 4707 4598 4769 4410 3889 1740 3856 5172 2832 2663 5527 4092 4585 4843 4270 4270 6082 4339 4458 4825 6228 3523 4352 ** 1.40 19.7 Hu sk co ve 2r 2 2 2 3 2 3 3 2 3 2 2 2 2 2 2 2 3 2 2 3 2 3 2 3 2 2 2 3 3 2 2 2 * 0.6 3.3 6 Pla nt asp ect 2 2 2 2 2 2 2 2 2 3 3 2 2 2 2 3 2 2 2 3 2 2 2 2 3 3 2 2 2 2 2 2 2 * 0.7 3.1 0 Ear asp ects Ins ects 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 3 2 2 2 3 2 2 2 2 3 3 2 2 2 2 2 2 2 * 0.5 4.8 8 1 0 1 0 6 2 4 2 6 5 4 5 5 2 1 1 3 3 1 3 2 1 6 2 2 1 2 3 5 4 2 1 3 * 3.5 7.0 1 21 Annexure 11: Performance of different maize varieties grown under varietal frontline demonstration at NMRP, Rampur, Chitwan in winter season of 2009 SN 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 Varieties Gaurav RML-4/NML-2 RML-3/NML-2 Arun-2/RML-8 C-1950 C-1921 C-6485 Poshilo Makai Shitala Narayani Upahar Ramur Composite Arun-2 Pool-17 Arun-1 EV Manakamana-1 Rampur SO3 FO6 Across 9942/Across 9944 Khumal yellow Deuti PoP 445 C1 Pool-16 S99TLYQ-A SOOTLYQ-1AB Rampur SO3 FO2 Arun-4 Gulmi-2 CP 666 CP 808 RC/RML-8 RL-176/RL-84 RL-111/RL-189 RML-62/RL-36 RML-55/RML-40 RC/RL-30-1 Bioseed 9681 Bioseed Manakamana-4 Rampur-2 S99TLYQ-A Across 9331 Arun-1 Manakamana-3 Rampur SO3 FO4 S99 TLYGHAYB (3) PoP 45/Pool-17 Gaurav CP 666 Bioseed Days to 50 % tasselling 92 91 89 62 80 79 80 85 79 71 69 70 55 50 54 56 76 76 65 75 55 62 74 75 82 65 81 79 78 72 77 86 84 69 74 70 76 75 61 76 67 59 88 84 78 67 89 82 85 Days to 50 % silking 95 95 93 66 84 83 84 88 83 74 72 74 58 53 58 58 80 80 69 79 59 65 78 78 86 69 85 83 81 76 81 90 88 74 78 74 80 78 64 79 71 63 91 88 84 70 92 85 89 Plant height (cm) 160 140 180 200 220 190 220 210 200 180 230 200 230 180 230 210 200 190 200 205 210 230 220 170 170 200 180 182 142 190 170 154 160 198 190 150 172 170 160 198 210 250 210 135 175 160 140 150 150 Ear height (cm) 90 80 110 110 100 98 116 105 105 115 130 130 120 70 122 98 110 118 110 102 99 130 130 110 100 108 100 97 70 98 99 80 80 107 88 80 92 81 92 92 108 70 87 82 100 80 82 81 70 Maturity date (days) 165 154 156 164 165 167 167 164 155 145 151 149 130 128 125 133 135 154 150 158 137 139 152 155 149 135 160 146 148 154 159 161 160 164 166 155 158 159 142 149 153 138 150 156 149 130 165 152 152 Plant aspect (1-5) 1.5 2 1.5 3 3.5 3 2.5 2.5 2 3 2.5 2 2 3 3 2.5 2 2 2 1.5 3 2 2 1.5 2.5 2.5 3 2 1.5 2.5 2 1.5 1.5 2.5 3 1.5 2 2 3.5 2 2.5 2.5 2 1.5 2 3 1.5 1.5 2 Ear aspect (1-5) 1.5 2 2 3 3 3.5 2 2.5 2 4 2 2 2 3 3.5 2 2 1.5 3 2 2.5 2.5 2 1.5 3 3 2.5 2 2 3 2.5 1.5 1.5 2.5 2.5 3 2 2 3 2.5 3 3 2 1.5 2.5 3 1.5 1.5 1.5 Grain yield (kg ha-1) 5324 7188 3557 4708 3393 5142 6293 5731 5857 3959 6749 4710 3759 3473 3543 3801 5323 7721 3970 4402 2670 2403 4690 5287 4758 3525 2129 4282 5101 4930 3893 5911 4253 4051 4642 5751 6154 4796 3851 3152 5429 2673 5036 4156 4307 3291 5250 7090 5293 22 Annexure 12: Adjusted mean grain yield (kg ha-1) of multinational hybrids evaluated with collaboration of NMRP, Rampur on three different locations in 2010-11 SN 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 Genotypes 10V10 10V20 30B11 30V92 900M gold 9220 9681 Aditya-929 All rounder Arun-2 Badshah gold Big Boss Bisco Bumper Bisco Heera Bisco Prince Bisco x 81 Bisco x 92 Biscox 97 gold C-1921 C-1945 C-1946 C-1950 C-6485 Challenge-1 Chhabili Commando CP-666 CP-808 CP-828 CP-838 Dekalb DK 984 Dekalb double DKC 9081 DMH-7314 DMH-849 Don 1588 Early-2 Godavari-989 Hi-Shell Kesherking-919 LY-558 LY-597 Madhur MM-1107 MM-1109 MM-7705 Naya 940 NMH 1242 NMH 666 (Sandhya) NMH 777 (Sunny) NMH 731 NMH 909 P 3404 P 3522 P 3540 P 3785 P 3856 Pinnacle Prabal Proagro 4640 Proagro 4642 Proagro 4794 Proagro Sampanna Rajkumar Rampur composite Parwanipur 8607 8284 6935 8772 8147 7729 7483 9041 8457 6455 6367 8711 9463 8373 10779 8042 8813 7284 9619 9167 7969 9791 8947 5841 7749 8160 8431 8173 8508 8795 10319 8107 9185 8447 8689 7797 8415 8382 7647 8219 7568 9972 9427 9610 10049 7006 10458 7952 6652 8299 9307 4890 8898 9448 8922 8224 9612 9539 8277 7799 9633 8146 8972 9509 6307 Rampur 5253 6852 10553 7935 7572 7074 8544 6239 8037 3854 8773 8295 4641 7050 7198 7945 5599 10179 5133 2735 7960 7646 6510 6586 7045 8969 7843 8453 5848 6945 7639 5757 7969 6272 6562 6898 4807 5022 8737 6443 8821 7768 4384 11562 9222 10151 5983 9754 7705 8162 7128 7365 8037 7370 7940 9892 11195 7067 10250 6425 7556 6415 8444 8233 5083 Tarahara 8560 9769 10411 8671 10126 9705 9963 6982 8032 4002 8347 8213 7883 4734 8867 7917 8187 9434 8009 9221 11720 8090 8107 8384 7770 10052 6296 5866 5688 5762 5113 6572 8765 7014 5563 6604 7658 8082 8105 8423 6877 7761 5398 6546 5609 7633 6692 10775 12185 9779 9932 9069 9969 8457 9085 7607 9607 10782 5288 8292 7745 7635 8019 8542 5850 Mean 7473 8302 9300 8459 8615 8169 8664 7421 8175 4770 7829 8406 7329 6719 8948 7968 7533 8966 7587 7041 9216 8509 7855 6937 7521 9061 7523 7497 6681 7168 7690 6812 8640 7244 6938 7100 6960 7162 8163 7695 7755 8501 6403 9239 8293 8264 7711 9494 8847 8747 8789 7108 8968 8425 8649 8575 10138 9129 7938 7505 8311 7399 8478 8762 5747 23 66 RML-4/NML-2 67 Samrat 1133 68 Samrat 1144 69 Shaktiman 70 Super 900M 71 T.C.S.-9696 72 Tx 369 Mean Replication Genotypes Location Location x Genotypes R2,% CV,% 6412 5600 7633 10223 7997 9545 8506 8397 * NS 9155 6587 5486 8636 6984 5596 9622 7408 NS ** 9255 7966 7027 9261 7564 10581 9626 8100 ** ** 45.9 23.7 51.4 28.4 68.4 21.3 8274 6718 6715 9374 7515 8574 9251 7968 NS ** ** * 53.3 25.7 24 Annexure 13: Combined analysis for grain yield and agronomic traits of maize genotypes under heat stress screening trials conducted at three different locations (Rampur, Nepalgunj and Parwanipur) in 2010 summer SN Genotypes Days to 50 % tasseling silking 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 RML-7 59 RL---84 56 RL--30-1 57 PUTU-13 61 Pool-17 45 Arun-1EV 47 Arun-4 57 POOl -16 51 Narayani 50 Upahar 61 Across-9331 53 S 97 TLYHGA YB (3) 52 Rampur composite 55 Mana-4 56 Arun-2 49 OEHPW 58 P501/RCO/P 502 SRCO 60 BGBY POP 60 S99TYQ-HGB 56 Posilo Makai-1 59 SO 1 SIWQ=3 64 S 99 TLYQ-B 61 Rampur SO 3 FO2 56 Rampur SO 3 FO4 56 Rampur SO3 FO6 57 Rampur SO3 FQ-02 62 BLSB SO7 F 10 54 BLSB SO 7 F12 57 TLBRS SO 7 F 14 55 TLBRS SO 7 F16 56 R POP-1 55 R POP-2 50 R POP-3 53 R POP-4 51 NML-1/NML-2 56 RML-4/NML-2 64 RML-8/RC 49 Mana-3 58 Rampur2 51 Deeuti 57 Pop-446 50 Khumal Yellow 51 Gulmi 54 Resunga Composite 54 Grand mean 55 CV (%) 5.40 SD 4.39 LSD (0.05) 3.36 F-test Genotypes (G) ** Location (L) ** Interaction (G × L) ** 64 59 61 67 47 50 62 55 52 62 56 54 57 57 53 61 64 65 58 64 66 66 59 59 60 65 56 60 58 60 57 53 56 53 57 67 51 60 53 60 53 55 56 57 58 5.70 4.83 3.74 ** ** ** ASI (days) Plant height (cm) Ear ht. (cm) Ear Len. (cm) Ear dia. (cm) Pl. Asp. (1-5) Ear Asp. (1-5) 4 3 3 3 3 3 3 3 2 5 2 2 3 4 1 2 3 6 1 4 3 2 3 3 4 4 5 3 2 1 2 2 4 3 1 2 2 4 3 5 3 4 2 4 188 166 165 139 161 167 171 165 170 190 173 173 186 182 173 203 172 184 175 187 164 177 190 169 192 183 174 183 190 188 191 196 178 191 187 166 184 191 171 192 154 177 234 200 180 9.56 15.29 20.63 ** NS NA 89 79 89 66 70 79 75 78 86 90 90 77 88 87 85 97 69 93 84 90 67 78 96 78 92 82 87 89 87 102 93 101 93 99 97 78 86 98 76 98 62 86 122 94 86 14.05 11.25 14.33 ** * NA 11 13 14 11 11 13 15 13 14 14 14 12 13 14 13 15 13 16 13 15 13 13 15 14 14 14 14 14 14 14 13 13 14 14 15 14 14 15 14 16 12 13 15 14 14 13.32 1.19 2.12 ** * * 4 3 4 3 3 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 6 4 4 4 4 4 4 4 4 20.7 0.40 0.93 NS NS NA 3 3 3 4 3 2 2 3 2 2 2 2 2 3 2 2 2 2 2 2 3 2 2 2 2 2 2 2 1 1 1 2 2 1 2 3 3 2 3 1 3 2 2 2 2 42.86 0.61 1.06 ** NS * 4 4 4 4 4 3 3 3 2 2 2 3 3 3 3 2 3 2 2 2 3 3 2 3 2 3 2 2 2 2 2 3 3 2 3 3 3 2 2 3 4 2 3 2 3 27.86 0.66 0.83 ** * ** 3 44.02 1.12 1.52 ** * NS ASI: anthesis-silking interval, pl:plant, ht: height, Len.: length,dia: diameter,Asp.:aspect and Gr.:grain, NS=Non significant, NA:Not available, * significant at 1% level and **significant at 5% level. Grain Yield (kg ha1 ) 2719 3675 2890 2671 3221 2943 3564 2927 4110 5228 3888 3069 4086 5299 3409 4451 3419 5110 4194 4636 3498 3419 4345 3982 4069 3910 4104 4466 4727 5144 4411 4453 4194 4799 4780 3555 4958 4206 3375 4185 1883 4366 3734 4631 3971 28.70 771 1299 ** ** NS 25 Annexure 14: Analysis for grain yield and agronomic traits of maize genotypes under heat stress screening trials conducted at Parwanipur in 2010 summer SN Genotypes 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 RML-7 RL-84 RL-30-1 PUTU-13 Pool-17 Arun-1EV Arun-4 POOl -16 Narayani Upahar Across-9331 S 97 TLYHGA YB (3) Rampur composite Mana-4 Arun-2 OEHPW P 501/RCO/P 502 SRCO BGBY POP S 99 TYQ-HGB Posilo Makai-1 SO 1 SIWQ=3 S 99 TLYQ-B Rampur SO 3 FO2 Rampur SO 3 FO4 Rampur SO 3 FO6 Rampur SO 3 FQ-02 BLSB SO7 F 10 BLSB SO 7 F12 TLBRS SO 7 F 14 TLBRS SO 7 F 16 R POP-1 R POP-2 R POP-3 R POP-4 NML-1/NML-2 RML-4/NML-2 RML-8/RC Mana-3 Rampur-2 Deeuti Pop-446 Khumal Yellow Gulmi Resunga Composite Grand mean CV (%) SD LSD (0.05) F-test Days to 50 % tasselling 58 64 62 57 46 49 48 51 55 56 52 54 54 58 51 56 59 60 56 58 66 64 57 58 58 68 54 58 55 57 54 53 53 52 57 68 49 58 51 56 50 51 54 52 56 6.75 4.99 7.56 ** Days to 50 % silking 63 70 64 65 47 52 50 53 60 60 55 57 56 61 55 59 64 65 58 63 68 69 60 61 61 71 56 59 58 60 56 54 56 53 58 71 52 60 54 59 53 55 56 56 59 7.40 5.63 8.75 ** Plant height (cm) 179 154 161 139 150 170 175 150 175 179 185 152 192 177 157 213 157 178 156 174 148 177 188 154 192 164 185 181 185 205 188 198 180 201 192 153 188 195 165 204 139 159 243 193 176 11.50 21.39 40.78 ** Ear height (cm) 92 79 90 71 65 79 84 70 92 85 101 70 101 102 81 108 60 98 80 91 59 79 87 75 96 75 96 90 91 114 103 110 94 113 98 75 91 110 76 116 62 83 137 95 89 15.95 16.57 28.60 ** Ear length (cm) 9 10 13 10 12 12 13 11 11 12 13 10 11 12 12 15 10 16 10 13 10 10 15 12 12 11 14 13 12 16 11 13 11 13 13 13 13 14 11 14 9 12 13 11 12 14.99 1.68 3.65 * Ear diameter (cm) 3 3 3 3 3 4 4 3 4 3 4 3 4 3 4 4 3 4 3 4 4 3 3 3 4 3 4 3 4 4 4 4 4 3 4 4 4 3 3 4 3 4 4 4 4 510.11 0.34 79.54 NS Plant aspect (1-5) 2 3 4 3 2 2 2 2 3 3 1 3 3 3 2 1 3 2 3 2 4 2 2 3 2 3 3 2 2 1 1 1 1 1 2 3 3 2 2 1 3 1 2 2 2 42.27 0.75 1.81 NS NS=Non significant, NA: Not available, * significant at 1% level and **significant at 5% level. Ear aspect (1-5) 4 5 3 3 4 3 3 4 3 3 2 4 3 2 3 2 4 2 3 3 3 4 3 3 3 4 2 2 2 2 2 2 3 2 3 4 3 2 3 3 5 2 4 3 3 31.23 0.74 1.79 NS Grain yield (kg ha1 ) 1839 1426 1889 3834 2599 2739 2972 2046 2847 3509 3990 2014 3262 3287 3666 3958 1482 4265 2311 3265 1881 1943 3213 2715 3079 2004 3908 3459 3629 4036 2339 4318 3845 3741 4400 2308 4336 4133 2876 2960 1644 3986 2880 3159 3045 28.84 874 1771 * 26 Annexure 15: Analysis for grain yield and agronomic traits of maize genotypes under heat stress screening trials conducted at Nepalgunj in 2010 summer SN Genotypes Days to 50 % tasselling 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 RML-7 RL-84 RL-30-1 PUTU-13 Pool-17 Arun-1EV Arun-4 POOl -16 Narayani Upahar Across-9331 S 97 TLYHGA YB (3) Rampur composite Mana-4 Arun-2 OEHPW P 501/RCO/P 502 SRCO BGBY POP S 99 TYQ-HGB Posilo Makai-1 SO 1 SIWQ=3 S 99 TLYQ-B Rampur SO 3 FO2 Rampur SO 3 FO4 Rampur SO 3 FO6 Rampur SO 3 FQ-02 BLSB SO7 F 10 BLSB SO 7 F12 TLBRS SO 7 F 14 TLBRS SO 7 F 16 R POP-1 R POP-2 R POP-3 R POP-4 NML-1/NML-2 RML-4/NML-2 RML-8/RC Mana-3 Rampur-2 Deeuti Pop-446 Khumal Yellow Gulmi Resunga Composite Grand Mean CV (%) SD LSD (0.05) F-test 60 56 62 64 46 49 49 56 54 56 55 54 54 62 52 56 58 58 57 58 66 61 57 59 59 68 53 58 55 56 54 52 53 52 57 66 49 58 51 56 50 50 54 52 56 5.7 4.88 6.43 ** Days to 50 % silking 66 60 64 72 47 52 51 58 58 60 58 57 56 63 55 59 64 63 58 63 68 66 60 63 64 71 56 59 59 60 56 55 56 53 58 70 52 60 54 60 53 55 58 55 59 5.8 5.49 6.85 ** Plant height (cm) Ear height (cm) Ear length (cm) Ear diameter (cm) Plant aspect (1-5) Ear aspect (1-5) Grain yield (kg ha-1) 191 171 155 126 157 169 161 174 181 190 170 173 184 176 181 189 178 190 192 189 170 171 179 173 190 190 160 172 192 182 180 200 173 184 185 164 184 185 171 185 150 181 221 214 178 6.2 16.07 22.17 ** 78 73 73 54 63 72 67 82 73 85 78 65 79 72 78 82 66 86 85 78 63 69 84 70 82 74 76 77 79 85 79 90 90 82 95 68 81 81 72 87 51 80 98 90 77 12.1 9.76 18.80 ** 11 13 16 10 7 14 17 15 16 17 15 15 14 16 13 16 15 17 14 17 17 14 16 16 17 16 15 14 16 15 15 11 16 15 18 16 16 15 16 18 11 14 17 17 15 13.7 2.21 4.14 ** 3 4 4 4 2 4 4 4 4 4 4 4 4 4 4 4 4 4 4 5 4 4 4 4 5 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 13 462.2 0.458 120.53 NS 3 4 3 4 4 2 2 3 1 1 1 1 1 2 2 2 1 1 1 2 2 1 1 1 3 1 2 1 1 1 2 3 3 2 1 4 1 2 3 2 3 1 3 3 2 19.9 1.00 0.76 ** 3 4 4 4 4 4 2 3 2 1 2 2 1 2 2 1 2 2 2 2 3 2 1 3 2 2 2 2 2 2 2 4 3 2 3 3 2 3 1 3 4 1 2 3 2 24 0.94 1.03 ** 2781 3523 4120 2507 3882 4263 4453 4407 5718 7376 5163 4590 5209 6377 3734 5921 5362 7370 5642 5118 7174 4619 6379 5574 6127 6305 4482 6188 5594 6158 6203 4371 5202 6339 6318 4802 6356 5134 3880 5788 2840 5439 5309 5734 5223 14.8 1175 1562 ** NS=Non significant, NA: Not available, * significant at 1% level and **significant at 5% level. 27 Annexure 16: Analysis for grain yield and agronomic traits of maize genotypes under heat stress screening trials conducted at Rampur in 2010 summer SN Genotypes 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 RML-7 RL-84 RL-30-1 PUTU-13 Pool-17 Arun-1EV Arun-4 POOl -16 Narayani Upahar Across-9331 S 97 TLYHGA YB (3) Rampur composite Mana-4 Arun-2 OEHPW P 501/RCO/P 502 SRCO BGBY POP S 99 TYQ-HGB Posilo Makai-1 SO 1 SIWQ=3 S 99 TLYQ-B Rampur SO 3 FO2 Rampur SO 3 FO4 Rampur SO 3 FO6 Rampur SO 3 FQ-02 BLSB SO7 F 10 BLSB SO 7 F12 TLBRS SO 7 F 14 TLBRS SO 7 F 16 R POP-1 R POP-2 R POP-3 R POP-4 NML-1/NML-2 RML-4/NML-2 RML-8/RC Mana-3 Rampur-2 Deeuti Pop-446 Khumal Yellow Gulmi Resunga Composite Grand mean CV (%) SD LSD (0.05) F-test Days to 50 % tasselling 54 51 60 62 44 44 43 44 43 55 53 48 58 49 44 61 62 63 55 61 60 59 56 51 54 51 54 57 54 57 57 46 54 50 54 58 49 58 51 59 51 52 54 57 53 6.8 5.65 4.23 ** Days to 50 % silking 56 54 60 65 47 47 43 46 47 58 55 50 60 50 49 65 64 66 55 65 62 63 59 55 56 54 56 61 56 60 60 49 56 53 56 59 51 60 52 62 53 54 56 61 56 7.1 5.76 4.67 ** Plant height (cm) 194 173 179 153 176 162 179 171 155 201 165 196 182 193 181 208 180 184 177 199 173 185 204 181 193 195 178 197 195 177 205 191 181 188 185 181 180 192 178 187 172 190 237 195 185 9.7 14.77 19.85 ** Ear height (cm) 97 87 104 74 83 85 74 82 92 101 93 97 84 87 95 103 80 96 88 101 80 86 118 88 97 99 88 102 91 108 99 102 97 102 99 91 85 104 81 92 74 97 132 97 93 16.3 11.27 16.01 ** Ear length (cm) 13 15 13 14 13 13 14 12 15 14 13 12 14 14 12 15 13 14 15 15 11 14 15 14 13 14 14 14 15 13 14 15 17 14 14 14 14 15 16 15 15 13 15 16 14 17.4 1.12 2.70 * Ear Plant diameter aspect (cm) (1-5) 4 3 4 2 4 3 4 4 4 4 4 3 4 3 4 4 4 1 5 3 4 3 4 3 4 3 4 3 4 4 4 3 4 3 4 2 4 1 4 2 4 4 4 2 4 3 4 2 4 2 4 2 4 2 4 2 4 1 4 2 5 1 4 2 5 2 4 2 4 3 4 2 5 4 4 1 4 3 4 2 4 3 4 3 4 2 4 1 4 2 6.28 48.5 0.24 0.93 0.49 1.18 NS ** NS=Non significant, NA: Not available, * significant at 1% level and **significant at 5% level. Ear aspect (1-5) 4 3 4 4 4 3 3 4 3 2 3 3 5 4 4 4 4 3 2 2 4 4 2 2 2 3 2 3 1 1 1 3 3 2 3 3 4 1 3 2 4 2 3 2 3 35.5 0.93 1.04 ** Grain yield (kg ha-1) 3538 6075 2663 1671 3181 1826 3269 2329 3766 4799 2510 2603 3786 6231 2827 3476 3414 3694 4629 5527 1439 3694 3443 3657 3001 3421 3922 3750 4958 5239 4693 4672 3535 4315 3623 3554 4183 3350 3369 3807 1164 3673 3013 5000 3643 39.2 1111 1769 * 28 Annexure 17: Grain yield and other traits of hybrids evaluated in CFFT in 2007 winter Genotypes RML4/NML2 NML1/RML8 RML3/NML2 RL30-1/RC GAURAV PIO30G10 Rampur composite Days to 50% anthesis 68 78 80 66 72 81 73 Days to 50% silking 71 81 83 69 75 84 76 Plant height (cm) 210 175 210 190 190 190 215 Ear height (cm) 110 70 110 105 95 90 105 Ear aspect (1-5) 1.5 2 2 1.5 2 2 2 TLB (15 1.5 2 1.5 2 2 2.5 2.5 Husk cover (1-5 1.5 2 2 2 2 2 2 Grain yield (kg ha-1) 8183 6705 6215 5395 6577 5276 5235 Source: Annual Report 2064/65 (2007/08). National Maize Research Program (NMRP), Rampur, Chitwan Annexure 18: Mean grain yield and other characters of hybrids tested in CFFT at Rampur, Parwanipur and Belachapi in 2008 SN Genotypes 1 Gaurav 2 Pioneer3087 3 Rajkumar 4 RML4/NML2 5 RML3/NML2 6 RML8/RC 7 Aditya 929 Grand mean F test LSD0.05 CV,% 50% silking days 107 106 107 111 110 102 102 107 NS 5.9 3.7 Plant height (cm) 130 135 134 133 137 136 119 132 NS 20 10 Ear height (cm) 55 46 48 52 57 52 42 50 NS 15.4 20 Grain yield (kg ha-1) 6368 6198 5817 5677 5178 5129 4493 5214 NS 2642 32 Source: Annual Report 2065/66 (2008/09). National Maize Research Program (NMRP), Rampur, Chitwan Annexure 19: Performance of different Indian and NWRP maize hybrids tested at Pithuwa, Chitwan in 2007 SN Genotypes Text color Grain texture Silking days Maturity days 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 Aditya Badshah Bioseed 9681 Keshar king Shubham 2 SOW 101 Rajkumar Pioneer 30G10 NML1/RML8 NML1/RML6 Pioneer 30G10/RML8 NML1/RML5 RML4/NML2 RML3/NML2 Gaurav Rampur composite Raja NMH 909 Hira Deluxe Bisko Bumper Makka 940 Kanak Super Deluxe Pioneer 30G07 Yellow Yellow Yellow Yellow Yellow Yellow Yellow Yellow Yellow Yellow Yellow Yellow Yellow Yellow Yellow Yellow Yellow Yellow Yellow Yellow Yellow Yellow SF SF SF SF SF SF F SD F F SF F F F F F F SD SF SD SD SF 116 108 111 106 108 106 109 111 118 115 108 123 119 124 121 114 120 120 122 120 119 121 156 153 154 151 153 151 153 155 155 159 153 160 157 160 157 156 158 158 159 158 158 160 Plant height (cm) 175 165 165 170 168 190 180 175 175 178 180 165 170 166 170 180 165 160 178 185 175 170 Ear height (cm) 90 80 62 100 70 85 80 90 75 85 85 80 95 80 100 95 70 85 75 110 85 90 Grain yield (kg ha-1) 6886 7256 6660 7626 7589 8070 8301 7885 6664 6293 6979 6367 5701 4776 5183 4035 6590 6960 6182 6664 6553 7330 Source: Annual Report 2064/65 (2007/08). National Maize Research Program (NMRP), Rampur, Chitwan, Nepal Husk cover (1-5) 1 3 2.5 2.5 2.5 2 1 1 1 2 1 1 1 1 1 1 2 2.5 1 2 1 1 29 Annexure 20: Response of hybrid maize (RML4/NML2) to different plant densities and nitrogen levels on growth and grain yield at Rampur in 2007 winter Density (D) Nitrogen (N) Plant height (cm) 142 144 149 148 145 150 132 146 149 145 NS NS NS Ear height (cm) 52 56 53 56 55 62 48 49 57 54.1 NS NS NS Ear length (cm) 14.1 15.8 15.8 14.6 16.2 16.2 14.0 16.3 16.1 15.4 NS * NS Kernel/row (number) Kernel rows/ear (number) 14.1 13.1 13.9 14.3 13.5 13.1 13.1 13.6 13.7 13.5 NS NS NS Thousands grains weight (g) 312.9 417.6 414.9 329.3 415.0 394.7 399.5 400.1 423.5 389.7 NS * NS Grain yield (kg ha-1) 3130 4520 4030 3890 4770 4840 4770 4980 4420 4360 NS * NS 30 x 60 60 32.6 30 x 60 90 31.8 30 x 60 120 30.6 25 x 60 60 29.4 25 x 60 90 33.5 25 x 60 120 33.0 20 x 60 60 29.9 20 x 60 90 31.3 20 x 60 120 32.4 Grand mean 31.6 Density NS Nitrogen NS Density x Nitrogen NS LSD0.05 for D LSD0.05 for D 0.8 43.4 0.63 LSD0.05 for D x N CV, % 6.09 12.53 5.57 8.05 5.79 11.18 14.47 Source: Annual Report 2064/65 (2007/08). National Maize Research Program (NMRP), Rampur, Chitwan Annexure 21: Mean grain yield of different hybrids with different plant spacing in winter 2008 Genotypes Spacing (cm) Grain yield (kg ha-1) 75 x 25 75 x 20 60 x 25 60 x 20 50 x 20 NML1/NML2 4235 4565 6438 6657 5603 5499 NML1/RML8 3791 4245 5707 5431 5374 4909 RML4/NML2 5224 5296 6516 7460 7056 6304 Mean 4416 4702 6220 6516 6001 5571 Source: Annual Report 2065/66 (2008/09). National Maize Research Program (NMRP), Rampur, Chitwan Annexure 22: Effects of different density and nitrogen levels on hybrid maize (RML4/NML2) at Rampur in 2008 Treatments Plant height (cm) 168 168 172 NS Ear height (cm) 78 79 82 NS Ear length (cm) 13.7 13.1 12.4 NS Kernels/rows (no.) Kernel rows/ear (number) 15.1 15.0 15.5 NS Thousands grains weight (g) 460 459 458 NS Stover Grain yield (kg yield (kg ha-1) ha-1) 30 x 60 cm 29.3 12250 8310 25 x 60 cm 28.2 12320 8510 20 x 60 cm 26.9 13230 9100 F test * * * LSD0.05 2.01 0.61 660 N=60 kg/ha 169 81 13.1 28.1 15.0 456 12180 8030 N=90 kg/ha 168 78 13.0 28.3 15.3 453 12800 8900 N=120 kg/ha 167 80 13.1 28.1 15.3 470 13820 8740 F test NS NS NS NS NS * * * LSD0.05 18.3 980 610 CV, % 3.91 3.88 5.92 8.13 4.14 5.05 16.3 13.31 Source: Annual Report 2065/66 (2008/09). National Maize Research Program (NMRP), Rampur, Chitwan, Nepal 30 Annexure 23: Details of the plant density study on hybrid maize in winter 2008 at Rampur Genotypes Plant spacing (cm) 75 x 25 75 x 20 60 x 25 60 x 20 50 x 20 75 x 25 75 x 20 60 x 25 60 x 20 50 x 20 75 x 25 75 x 20 60 x 25 60 x 20 50 x 20 NML1/NML2 NML1/RML8 RML4/NML2 Grand mean Variety (A) Population (B) AxB LSD0.05 CV, % 50% anthesis 50% silking 65 64 66 66 67 66 65 66 66 66 64 63 65 64 63 65 * NS NS 3 4.2 73 72 74 72 74 74 72 72 74 73 71 70 71 70 69 72 * NS NS 4 5.2 Plant height (cm) 209 226 223 227 223 197 216 201 206 217 219 217 208 225 216 215 NS NS NS 19 6.0 Ear height (cm) 110 124 132 124 124 117 114 115 119 120 137 118 123 131 118 122 NS NS NS 298 8.1 Root lodging (%) 3 1 1 0 3 1 0 2 1 1 0 0 0 1 0 Shoot lodging (%) 1 2 2 6 3 1 0 1 2 3 0 1 2 0 0 Grain yield (kg ha-1) 4235 4565 6438 6657 5603 3791 4245 5707 5431 5374 5224 5296 6516 7460 7026 5571 ** ** NS 1119 12 Source: Annual Report 2065/66 (2008/09). National Maize Research Program (NMRP), Rampur, Chitwan, Nepal Annexure 24: Interaction effect of Hybrid and spacing on grain yield (kg ha-1) Factor B (Population levels) 75 × 20 cm 75 × 25 cm (53,333 (66,666 plant/ha) plant/ha) 60 × 20 cm (83,333 plant/ha) Average (Hybrids) 5490 5.43 5060 5140 NML-1/NML-2 75 × 15 cm (88,888 plant/ha) 4590 RML-8 /RC RML-4 /NML-2 4350 6190 5390 6077 5.05 6.05 3750 6030 4640 6240 Average (spacing) 5040 5860 5.51 4950 5340 Factor A (Hybrid) Annexure 25: Interaction effect of inbred and nitrogen on grain yield (kg ha-1) Factor A (Inbred line) NML-2 RL-105 RL-111 RML-4 Average (Nitrogen level) 120:60:40 NPK (kg ha-1) 1420 1190 1380 1220 Factor B(Nitrogen level) 150:60:40 NPK (kg ha-1) 1250 820 1650 800 200:60:40 NPK (kg ha-1) 1440 1170 1040 1200 1300 1130 1220 Average (Inbred lines) 1370 1060 1360 1070 1220 31 Annexure 26: Treatment combination for N x D experiment SN 1 2 3 4 5 6 7 8 9 Treatments D1N1 D1N2 D1N3 D2N1 D2N2 D2N3 D3N1 D3N2 D3N3 Nitrogen (N) kg ha-1 60 90 120 60 90 120 60 90 120 Plant population (ha) 55,555 plants 55,555 plants 55,555 plants 66,666 plants 66,666 plants 66,666 plants 83,383 plants 83,383 plants 83,383 plants Spacing (cm) 30 x 60 30 x 60 30 x 60 25 x 60 25 x 60 25 x 60 20 x 60 20 x 60 20 x 60 Remarks 10 t of compost and 40:30 kg/ha of P2O5 and K2O were applied basally in all the plots. Annexure 27: Effect of nitrogen rates on the growth and yield characters of maize planted at varying densities (N x D) in the years 2009/10 at NMRP farmland, Rampur Treatments Treatment combination Plant ht, cm Ear ht, cm Ear length (cm) No. of kernel rows/ ear No. of kernel/ row Stover weight (kg ha-1) T1 T2 T3 T4 T5 T6 T7 T8 T9 Grand mean CV (%) F-test D1N1 D1N2 D1N3 D2N1 D2N2 D2N3 D3N1 D3N2 D3N3 148.66 168.00 168.33 162.66 174.66 173.00 165.33 170.66 177.66 167.66 4.61 * ** NS 7.76 8.46 - 59.66 70.43 78.00 67.33 79.66 73.33 68.33 74.00 79.66 72.27 9.46 NS * NS 7.00 - 12.26 14.80 14.06 13.33 13.53 14.33 12.80 13.53 14.06 13.63 2.51 NS ** * 0.355 0.512 13.33 16.13 15.86 15.00 16.13 16.53 15.60 16.00 16.40 15.66 3.89 * ** * 0.678 0.626 1.085 27.53 32.53 32.80 30.13 32.20 32.60 29.06 30.60 33.06 31.17 5.86 NS ** NS 1.875 - 6060 8760 8700 6830 9100 8900 6900 8800 8660 8080 5.42 NS ** 448 - LSD (0.05) Density, a Nitrogen, b axb a b axb 1000 grain weight (g) 410.66 445.33 456.33 425.33 456.66 477.00 410.66 448.33 456.33 442.96 2.44 ** ** NS 10.06 10.06 - **=Highly significant, *= significant and ns= non-significant. Annexure 28: Effect of nitrogen rates on the growth and yield characters of maize planted at varying densities (N x D) in the years 2009/10 at NMRP farmland, Rampur. Density Nitrogen Grain yield (kg ha-1) 60 x 30 60:60:40 2747 60 x 30 120:60:40 3857 60 x 30 180:60:40 3870 60 x 25 60:60:40 2790 60 x 25 120:60:40 4437 60 x 25 180:60:40 4697 60 x 20 60:60:40 3113 60 x 20 120:60:40 4480 60 x 20 180:60:40 4360 Grand mean 3816 CV, % 18.6 LSD0.05 1.2 Source: Annual Report 2067/68 (2010/11). National Maize Research Program (NMRP), Rampur, Chitwan, Nepal Grain yield (kg ha-1) 8980 11010 10990 10170 12100 11470 9130 12160 12040 10890 8690 NS ** NS 0.846 - 32 Annexure 29: Screening of maize inbreds against Northern Leaf Blight (NLB) at Rampur in 2006 SN Inbred 1 RL163 2 RML50 3 RL100 4 RL41 5 RL162 6 RML12 7 RML4 8 RL141 9 RL143 10 RL47 11 RL169 12 RML18 13 RL98 14 RL19 15 RML9 16 RL134 17 RL9 18 RML13 19 RL35A 20 RL106 21 RML36 22 RML5 23 RL194 24 RL168 25 RML41 26 RML11 27 RL171 28 RL167 29 RL116 30 RML3 31 Yellow pop corn 32 RML46 33 RL140 34 RL95 35 RL118 36 NML1 37 RL84 38 RL111 39 RL177 40 RL99 41 RL14 42 RL57 43 NML2 44 RML2 45 RML59 46 RML40 47 RML6 48 RL31 49 RL161 50 RL110 51 RML19 52 RML55 53 RL17 54 RL30-1 Grand mean F test LSD0.05 CV, % 1st score (%) blighted 21.5 15 15 12.5 10 10 17.5 22.5 15 8 15 12.5 20 17.5 9 8.5 10 17.5 16.5 12 6.5 7.5 7.5 11 17.5 21.5 18 9.5 13.5 5 16 12.5 13.5 17.5 20 6.5 8 11.5 6.5 9 5 10 12 10 10 6.5 5.5 10 7.5 5 8.5 5 6.5 5 11.7 ** 6.5 28 2nd score (%) blighted 40 47.5 37.5 40 42.5 35 40 45 45 20 40 32.5 42.5 40 20 37.5 35 35 37.5 27.5 15 25 22.5 37.5 42.5 40 37.5 27.5 35 32.5 47.5 45 42.5 45 42.5 22.5 35 35 35 37.5 32.5 30 37.5 40 35 32.5 45 40 22.5 27.5 35 30 27.5 25 35.2 ** 10.6 15 3rd score (%) blighted 47.5 47.5 47.5 45 52.5 37.5 40 55 50 35 40 35 55 52.5 40 42.5 42.5 35 37.5 32.5 27.5 32.5 32.5 45 55 45 40 35 42.5 32.5 62.5 52.5 50 40 42.5 27.5 40 47.5 40 40 37.5 37.5 45 45 40 37.5 47.5 40 35 35 37.5 35 37.5 37.5 41.6 ** 12.8 15.4 Source: Annual Report 2063/64 (2006/07). National Maize Research Program (NMRP), Rampur 33 Annexure 30: Response of maize genotypes against major diseases at NMRP, Rampur in 2009 summer SN Genotypes 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 NML-1 NML-2 RML-3 RML-4 RML-5 RML-8 RL-105 RL-84 RML-17 RML-18 PUTU-13 RL-30-1 CML-161 CML-187 CML-172 AG-27 L-1 L-7 POOL-17 ARUN-1EV ARUN-4 POOL-15 POOL-16 NARAYANI UPAHAR ACROSS-9331 S97TLYGHAYB(3) RAMPUR COMPOSITE MANAKAMANA-4 ARUN-2 AC 9942/AC 9944 OEHPW P501SRCO/P502SR BGBYPOP S99TLYQ-AB S99TLYQ-HG-B POSHILO MAKAI-1 SO1SIWQ-3 S99TLYQ-B RAMPUR SO3FO2 RAMPUR SO3FO4 RAMPUR SO3FO6 RAMPUR SO3FQ02 BLSBRSO7F10 BLSBRSO7F12 TLBRSSO7F14 TLBRSSO7F16 R POP-1 R PO-4 R POP-2 R POP-3 NML-1/NML-2 RML-4 /NML-2 1st score 3.3 2.5 2.3 3.3 2 1.5 2.8 2.5 3 2.5 1.5 2.5 3.3 3 3.5 2 2 1.8 3.5 3.3 2.8 3 2.8 3 2 2.5 3 2.8 3 3.8 2.3 2 3.3 2.5 2.8 2.3 2.5 2.8 3.3 2 2.3 2.5 2 2 2 2.3 2.3 3.3 2 2.5 2 1.8 2 BLSB Score (1-5) 2nd score 3.8 2.8 2.8 3.8 2.5 2 3 2.8 3.3 3.3 2.5 2.8 3.8 3.5 3.8 2.3 2.3 2.3 4 3.5 3.3 3.8 3.3 3.3 2.8 2.5 3.5 3.8 3.5 3.5 2.8 3 3.5 2.8 2.5 3 3 3 3.3 2.5 2.5 2.8 2.8 2.5 2.3 2.5 2.5 3.5 2.8 3.3 2.3 2.3 2.3 3rd score 4 3.3 2.8 3.8 2.5 2.5 3.5 3.3 3.8 3.8 2.8 3.3 4.8 4 4.8 3 2.5 2.3 4.3 4.3 4.3 4.3 4.3 3.3 3 3.5 3.5 3.8 3.8 4.3 3 3.3 4 3 3 3.5 3.3 3.3 3.8 2.8 2.8 3 2.8 2.5 2.5 3 3 3.8 3.5 3.5 3.5 2.5 2.3 % rotten ears 52 38 10 7 8 82 54 31 67 63 48 44 60 50 23 6 21 37 88 85 37 111 53 43 41 47 64 28 74 66 26 21 39 26 41 53 48 16 29 23 22 33 42 31 24 22 28 33 6 24 34 15 29 H. maydis (1-5) 2.3 1.8 2.3 1.8 2 2 2.3 2.5 2.5 2.3 2 3 3 2 3.3 2 2.3 2.3 2.3 3.3 3.3 1.8 2.3 2.5 2.5 2.5 2 2.3 2.3 2.5 3 2.3 2 2 2.3 2.8 2.8 2.3 2.8 1.8 1.5 2.5 2.5 1.8 2 2 2 2.3 2.5 2.5 1.8 1.8 1.8 Late wilted plants 50 (%) 56 40 56 51 32 54 40 28 58 35 67 56 50 47 45 78 44 44 47 28 38 38 58 49 47 60 55 63 38 31 42 43 59 49 46 74 73 61 61 46 51 54 44 38 42 44 50 57 39 39 18 35 34 54 55 56 57 58 59 60 61 62 63 RML-3 /NML-2 RML-8 / RC MANAKAMANA-3 MANAKAMANA-1 DEUTI POP-446 KHUMAL YELLOW GULMI-2 RESUNGA COMPOSITE L-13 Grand mean F-test CV (%) LSD (0.05) 2.5 2.8 2.3 3 2.8 2.8 3.5 2.8 2.8 1.8 2.6 ** 19.96 1.02 2.8 3 2.5 3.5 3 3.5 4 3.5 3.5 2.3 2.9 ** 17.6 1.05 3 3.5 3 4.3 3.8 3.8 4.5 3.8 3.8 2.5 3.4 ** 18.78 1.27 25 8 20 65 44 50 27 36 35 8 38.4 ** 58.9 45.25 2 2 2 2.5 2.3 3.3 2.8 3.3 3.3 1.8 2.3 ns 27.9 1.29 26 37 48 51 54 47 61 47 43 62 48 ns 46.8 45.8 Annexure 31: Combined analysis on pest infestation of maize genotypes under heat stress screening trials conducted at three different locations (Rampur, Nepalgunj and Parwanipur) in 2010 summer SN Genotypes 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 RML-7 RL-84 RL-30-1 PUTU-13 Pool-17 Arun-1EV Arun-4 POOl -16 Narayani Upahar Across-9331 S 97 TLYHGA YB (3) RC Manakamana-4 Arun-2 OEHPW P 501/RCO/P 502 SRCO BGBY POP S 99 TYQHGB Posilo Makai-1 SO 1 SIWQ-3 S 99 TLYQ-B Rampur SO 3 FO2 Rampur SO 3 FO4 Rampur SO 3 FO6 Wilt % Stem borer % 20.17 3.83 6.0 1.17 11.0 12.83 6.17 2.0 9.0 3.83 1.67 2.50 0.00 5.0 2.17 8.33 1.67 3.17 2.67 0.83 6.83 0.0 7.0 1.83 8.50 1.50 6.33 7.0 0.0 7.17 1.17 0.72 8.83 5.83 6.00 0.83 1.83 2.0 5.50 11.17 19.0 4.0 0.0 0.83 6.33 2.33 10.83 2.33 5.17 2.67 SN 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 NS=Non significant, NA: Not available, * significant at 1% level Genotypes Rampur SO 3 FQ02 BLSB SO7 F 10 BLSB SO 7 F12 TLBRS SO 7 F 14 TLBRS SO 7 F 16 R POP-1 R POP-2 R POP-3 R POP-4 NML-1/NML-2 RML-4/NML-2 RML-8/RC Mana-3 Rampur-2 Deeuti Pop-446 Khumal Yellow Gulmi Resunga Composite Grand mean CV (%) SD LSD (0.05) F-test Genotypes (G) Location (L) Interaction (G × L) Wilt % Stem borer % 2.33 0.0 3.17 6.0 2.0 7.17 8.33 9.0 18.33 8.33 14.50 4.17 1.50 0.83 0.0 0.0 0.0 0.0 1.17 0.83 2.50 8.0 3.0 0.0 7.0 4.0 4.67 10.33 0.0 2.17 0.0 0.0 6.33 0.0 1.50 2.0 11.17 0.0 7.11 131.67 4.687 1.94 266.64 2.313 10.89 5.827 * NS NA NA NS NA 35 Annexure 32: Response of maize genotypes to maize stem borer, C. partellus Swinhoe. SN Designations OPV 1 S99TLYQ-AB 2 R.POP-2 3 R.POP-1 4 EEYC1 5 ARUN 4 6 S97TLYGH'A×B'(3) 7 S97TEYGH'A×B'(3) 8 POP45/POOL 17 9 RAMPUR SO3FO4 10 POP44/POOL 15 11 ARUN-2 12 POP 446C1 13 KHUMAL YELLOW/POOL 17 14 UPAHAR 15 ACROSS 9331 16 POP 445C1 17 POOL 17 18 RAMPUR SO3FO2 19 NARAYANI 20 O2SAVDIF 2 21 ACROSS 9942/ACROSS 9944 22 RAMPUR COMPOSITE QPM 23 SOOTLYQ-AB 24 SOISIWQ-1 25 SO3TLYQ-AB-2 26 SO3TLYQ-AB-O1 27 SOISIWQ-3 28 SOOTLYQ-B Hybrids 29 Rampur composite / RML-8 30 BIOSEED 31 RML 4 / NML 2 32 GAURAV F test CV% LSD at 0.5 level Insect score(0-9 scale)* Percent insects damage** Tunnel length* Exit holes* 4.0 3.7 2.3 2.0 3.0 3.7 3.7 4.7 2.7 2.3 3.3 4.3 5.7 3.3 3.0 3.3 4.3 4.0 2.3 4.3 3.0 3.0 27.7(5.31) 26.4(5.19) 31.6(5.66) 23.6(4.91) 27.9(5.33) 26.9(5.23) 21.6(4.70) 29.5(5.48) 24.1(4.96) 21.6(4.70) 25.6(5.11) 40.3(6.39) 40.5(6.40) 32.0(5.70) 20.9(4.63) 26.9(5.23) 37.3(6.15) 42.5(6.55) 20.3(4.56) 31.0(5.61) 21.7(4.71) 35.0(5.96) 2.2(1.64) 2.8(1.82) 5.1(2.37) 0.7(1.09) 1.1(1.26) 0.3(0.89) 1.3(1.34) 0.7(1.09) 3.1(1.90) 1.8(1.52) 2.0(1.58) 1.3(1.34) 2.7(1.79) 1.8(1.52) 0.1(0.77) 3.3(1.95) 4.5(2.24) 2.8(1.82) 1.4(1.38) 0.4(0.95) 3.5(2.00) 1.3(1.34) 0.3(0.89) 0.1(0.77) 0.7(1.09) 0.8(1.14) 0.3(0.89) 0.3(0.89) 0.6(1.05) 0.2(0.84) 0.3(0.89) 0.3(0.89) 0.7(1.09) 0.0(0.71) 0.4(0.95) 0.5(1.00) 0.2(0.84) 0.5(1.00) 0.5(1.00) 0.1(0.77) 0.1(0.77) 0.1(0.77) 0.3(0.89) 0.1(0.77) 6.7 4.3 4.0 3.7 3.3 2.0 39.7(6.34) 36.9(6.11) 21.2(4.66) 32.9(5.78) 23.1(4.86) 29.9(5.51) 1.0(1.22) 8.6(3.02) 4.5(2.24) 0.07(0.75) 0.00(0.71) 0.1(0.77) 0.0(0.71) 0.1(0.77) 0.2(0.84) 0.0(0.71) 0.1(0.77) 0.0(0.71) 1.3 3.0 2.3 4.7 * 43.54% 2.472 14.2(3.83) 26.0(5.15) 20.9(4.63) 34.9(5.95) * 19.92 1.719 8.4(2.98) 12.2(3.56) 0.5(1.00) 1.2(1.30) ** 41.26 1.024 0.4(0.95) 0.5(1.00) 0.0(0.71) 0.1(0.77) ** 18.49 0.269 *Means of three replications. ** Mean of three replications and two observations. -Figure in the parentheses is the transformed value of original value by Square root of (X +0.5).