Biochemical TestsCommonly Used Tests for Aerobic, Gram-positive Cocci Catalase positive Catalase negative Taxos A positive Vancomycin positive Taxos P or Optochin positive Taxos P or Optochin negative Camp positive Camp negative Bile Esculin positive Bile Esculin negative 6.5% NaCl positive 6.5% NaCl negative Bile solubility positive Bile solubility negative . PYR & LAP positive PYR negative & LAP positive Coagulase positive Coagulase negative . Additional Tests for Aerobic. Gram-positive Cocci Novobiocin (left) and Bacitracin (right) sensitive Novobiocin (left)and Bacitracin (right) resistant Rapid Hippurate Hydrolysis positive Rapid Hippurate Hydrolysis negative Thermonuclease Test agar positive Thermonuclease Test agar negative Pyruvate Trehalose-Mannitol Broth . ESC positive) Bacti Card Strep (LAP positive. PYR. PYR & ESC negative) .Modified Oxidase (Microdase Disk) positive MGP (Methyl-alpha-D-Glucopyranoside) Pigment Bacti Card Strep (LAP. Commonly Used Tests for Aerobic. Gram-Negative Cocci Oxidase positive (Gordon & McLeod's Reagent) Polysaccharide from Sucrose positive Rapid Sugars Polysaccharide from sucrose negative . Standard Biochemical Tests for the Enterobacteriaceae Indol Voges-Proskauer Methyl Red Simmons Citrate agar Triple Sugar Iron agar (TSI) Lysine Iron agar (LIA) Decarboxylase Broth (Moeller) Christensen Urea agar . Motility Phenylalanine Phenyl Red Carbohydrate Broth Gas production Malonate Broth . Standard Biochemical Tests for the Nonfermentative Gram-negative Bacilli OF sugar positive OF sugar negative Nitrate positive (before zinc) OF sugar (reaction of fermentative bacilli) Nitrate positive (after zinc) Nitrate negative . Triple Sugar Iron agar (TSI) Motility Decarboxylase Broth (Moeller) positive 42° C positive 42° C negative Oxidase positive (Kovacs reagent) Decarboxylase Broth (Moeller) negative Acetamide positive Acetamide negative Oxidase negative (Kovacs reagent) . Tests for Haemophilus XV positive Porphyrin (ALU) positive Satellitism V only positive Porphyrin (ALU) negative Oxidase positive . not an arrow) Positive Motility Test (resembles an umbrella or christmas tree) .Key Tests to rule out Bacillus anthracis Positive Motility Test Lecithinase negative (Right plate) Penicillin (10 units) sensitive Key Tests for Listeria monocytogenes Positive Camp Test (resembles a shovel. Positive Bile Esculin Test Triple Sugar Iron agar (TSI) Key Tests for Clostridium perfringens Special Potency Antimicrobial Disks Lecithinase positive (Left plate) Reverse Camp Test positive (Arrow points toward Clostridium isolate) . Key Tests for Bacteroides fragilis group BBE (Bacteroides Bile Esculin agar) growing B. fragilis group Thioglycolate with Bile positive Esculin Broth positive Special Potency Antimicrobial Disks . Genera Laktoz a Saharoz a M R V P H2 S Indo l Pokre tljivos t Citra t Ure a Escherichia coli + d + - - + + - - Edwardsiella tarda Citrobacter freundii Citrobacter intermedius biotype a Citrobacter intermedius biotype b Klebsiella pneumoniae Klebsiella ozenae *gde je oxytoca Klebsiella rhinoscleromati s Enterobacter cloacae Enterobacter aerogenes - - + - + + + - - + d + - + - + + (+) + d + - - + + + (+) + d + - - + + + (+) + + - + - d - d d (+) + + - - d - d d - + + - - d - d d + + - + - - + + d + + - + - - + + d Hafnia alvei - - - + - - + + - Serratia marscences - + - + - - + + - Proteus vulgaris - + + d + + + - + Proteus mirabilis - (+) + d + - + d + (+) slow positive. d varies for different strains .