Content - SommairePage Partners and sponsors – Partenaires et parrainage ICVG Steering Committee – ICVG XVI Organisation Foreword – Avant propos Acknowledgements - Remerciements Content - Sommaire 2 4 5 6 7 Session I - Introductory keynotes Oral Martelli G. P. Grapevine Virology Highlights 2006-09 Gugerli P. 25 years of serological identification of grapevine leafroll-associated viruses: antiserum and monoclonal antibodies to GLRaV-1 to GLRaV- 9 15 24 Dolja V. V. New developments in understanding gene functions and evolution of the grapevine Closteroviruses 29 Session II – Detection – Plant material and Virus source Oral Invited lecture Sabanadzovic S. Viruses of native Vitis germplasm in the Southeastern united states Comparison of High Throughput Low Density Arrays, Rt-PCR and Real-Time Taqman® RT-PCR in the detection of grapevine viruses Osman F., C. Leutenegger, D.A. Golino, A. Rowhani Grapevine viruses in Chile: Multi-parallel detection based on metagenomic strategies Engel E. A., P. F. Escobar, P. Rivera, P. D.T. Valenzuela. 32 36 38 Grapevine virus collection at Nyon: A contribution to a putative network of a worldwide grapevine virus reference collection Gugerli P., J-J. Brugger, M-E. Ramel, S. Besse. 40 Poster 01 Poster 02 Grapevine virus Q: The first plant virus with a permuted active site of RNA-dependent RNA polymerase Sabanadzovic S., N. Abou Ghanem-Sabanadzovic, A. E. Gorbalenya 42 Production of monoclonal antibodies to Grapevine leafroll- associated virus 9 (GLRaV-9) Gugerli P., S. Rigotti, M-E. Ramel, N. Habili, A. Rowhani, W. Bitterlin, S. Besse. Detection of viruses in seeds of infected grapevines with an emphasis on Grapevine rupestris stem pittingassociated virus Habili N., R. Davies, J. Randles. Real time PCR for sensitive reliable grapevine virus detection Malan S., M-J. Freeborough, J. Burger Prevalence of seven grapevine viruses in table grapes from Vinalopó (Alicante) determined by ELISA and Real-Time RT-PCR Bertolini E., C. Martínez, J. García, J. Juárez, A. Martínez, E. Vidal, A. Yuste, M. Albiach-Martí, M. Cambra, A. Olmos. Virus-derived sequences in the nuclear genome of grapevine Bertsch C., M. Beuve, V. V. Dolja, M. Wirth, F. Pelsy, E. Herrbach, O. Lemaire How widespread is the presence of Grapevine leafroll-associated virus 8 (GLRaV-8) putative capsid protein gene sequences in DNA and RNA obtained from grapevine varieties? Esteves F., M. Teixeira Santos, M. De Lurdes Rocha, J. E. Eiras-Dias, F. Fonseca The occurrence of viruses in the clonal selection vineyards in Czech republic Holleinová V., L. Bláhová, K. Baránková Contribution of new certified clones to the improvement of the italian table grape industry La Notte P., C. Pirolo, P. Giannini, G. Bottalico, A. Campanale, V. Savino, G. P. Martelli Clonal selection and sanitary status of local grapevine germplasm in Serbia Mandi B., Ž. Ivanovi, L. Susca, G. Bottalico, M. Starovi, D. Jakši, S. Kuzmanovi, D. Ivaniševi, V. Gavrilovi, Kora N., Digiaro M., P. La Notte 44 46 Poster 03 Poster 04 Poster 05 48 50 Poster 06 Poster 07 52 54 Poster 08 Poster 09 Poster 10 56 58 60 Progrès Agricole et Viticole, 2009, Hors Série – Extended abstracts 16th Meeting of ICVG, Dijon, France, 31 Aug – 4 Sept 2009 N Fiore Genetic variability within the coat protein gene of Grapevine fanleaf virus (GFLV) isolates from South Africa Liebenberg A. N.Poster 11 Old grapevine varieties vineyards: a window over a pre sanitary selection era and a source of viruses Teixeira Santos M... S. Hofmann. Lemaire. M. A. Kuzmanovi Characterization of Raspberry bushy dwarf virus isolates from grapevine Mavri Pleško I. E. Y. F. Wetzel Recombination events in Rna-2 of Grapevine fanleaf virus and Arabis mosaic virus in grapevines affected by Yellow mosaic Jawhar J. Li. Ritzenthaler. Maghuly . J.M. C. Keichinger. Didier. P. Boutant. Laimer Distribution of Grapevine fanleaf virus (GFLV) in grapevines during the season epin U. Komar. Cabaleiro. S. M. A. F. Lemaire Nicotiana benthamiana plants expressing viral small interfering RNA show differential resistance level against GFLV Bruno C. M. Schmitt-Keichinger. D. M. La Notte. P. Saldarelli.T. M-J. Fernandes-Calvino. Viršek-Marn. C. P. M. J. Gianinazzi Evidence of recombination in 2A gene from a Grapevine fanleaf virus isolate Nourinejhad Zarghani S. P. M. Pirolo.D. O. A... V. Lorber.. S. Pino. Hita. C. S. Sokhandan Bashir 64 69 71 73 75 77 79 81 83 85 86 Poster 15 Poster 16 Poster 17 88 90 92 Poster 18 94 Poster 19 Poster 20 96 98 Poster 21 Poster 22 100 102 . Savino. L. P. Castellano. F. Esmenjaud. Legorburu Absolute quantification of Grapevine fanleaf virus in Chenopodium quinoa using real-time RT-PCR assays Ulloa S. P. Eiras-Dias 62 Session III .. Bergdoll. Fallanaj. C. Padilla. Van Tuinen. E.. Minafra. C. Bellstedt. Mély. Vigne. Pompe-Novak. M. Mansilla. Keller. C. C. Dunoyer. M. J Visser. C. V. A. Himber. Esteves. O.D.. C. Gugerli. B. Thomas. Martelli The specificity of GFLV and ARMV transmission by their respective nematodes: from structure to function Schellenberger P. Demangeat.. M. Bron. Andret-Link. C. L. M. Fuchs. Freeborough. F... J. Delpasand Khabbazi Poster 12 Poster 13 Poster 14 Real-time PCR of Grapevine fanleaf virus Bláhová L. Pazhohande. Fayolle. F. Fonseca. Pidra First records of Arabis mosaic virus (ARMV) on grapevine in Spain Abelleira A.U.. Digiaro.. Heinlein. G. A. Ritzenthaler Symptom determinants on the Arabis mosaic nepovirus genome Dupuis L. M. Nepovirus transmission by Longidoridae nematodes with special emphasis on the pair Grapevine fanleaf virus/Xiphinema index Identification of plasmodesmata located receptors involved in the movement of Grapevine fanleaf virus (GFLV) and other viruses employing tubule-guided movement Amari K.T.. P. Besse. A. Marmonier. Urek Isolation of movement protein gene by the use of degenerate primers from Iran isolates of Grapevine fanleaf virus and assessment of the genetic diversity Sokhandan Bashir N.T. Burger Mycorrhiza reduce development of the nematode vector of Grapevine fanleaf virus in soil and root systems Hao Z. A.. Krsmanovi. Demangeat. Valenzuela . T.. Guedes Da Silva. V. C. M. Shams-Bakhsh. G. M. A. M. E. Širca. D. Maule. V. G. D. G. Trapani. De Lurdes Rocha.. Valenzuela Genetic diversity of the coat protein gene of Chilean isolates of Grapevine fanleaf virus Zamorano A. S. Boscia. Bruno Detection of Virus like particles (Vlps) by ISEM in transgenic grapevines expressing different GFLV CPconstructs Gottschamel J. P. P. S. I. P. C. Sauter. P.Fanleaf and other spherical viruses Oral Invited lecture Demangeat G. X. Ritzenthaler Partial RNA-2 Sequence of Grapevine Bulgarian latent virus Elbeaino T. D. Bassler. J. Gianinazzi-Pearson. A. Olmos. Ravnikar Comparisons of genomic and pathological features among barley and grapevine infecting-isolates of Arabis mosaic virus Marmonier A.. C. Lerich. B. J. G.. R.. Paltrinieri. P. M. S. P. López. Olufemi. Triolo The virus status of grapevine mother plantations in Cyprus Neophytou G. Roumi. Bouyahia. E.. D'Onofrio.Phytoplasma diseases Invited lecture Malembic-Maher S. Semenescu A survey for endemic and exotic viruses of Australian grapevines Constable F. Mitrovi. B.T. J.A. M. -2. N. Gu. J. X. Zottini. E. M. K. Veratti. Mannini. López.. Cibriaín. Rodoni Preliminary results on the quantification of different grapevine viruses in a typical northwestern italian cultivar Pacifico D. F. M. P. Seitz On host races and co-evolution of the grapevine yellows vector Hyalesthes obsoletus and stolbur phytoplasma Johannesen J. S. F. A. L.V. H. J. Santovito. Johannesen.. F. N. S. Padilla V. Nicholas. Borgo...R. González-Rodríguez. Aguirrezábal..N. Izadpanah Occurrence of grapevine leafroll-associated virus-1 and 3 in Croatian autochton grapevine varieties from Dalmatia Voncina D. N. A. J. M. V. N.. E. V. C. vectors and host plants in the two epidemic systems of Bois noir Maixner M. E. România Buciumeanu E-C. V.R. Gutha. L. F. Bahrami Kamangar. P. Nancarrow. Maixner 135 137 139 141 143 Progrès Agricole et Viticole. C.C. Dal Molin. 31 Aug – 4 Sept 2009 . B. S.R.. Padilla. Nicholas. N. France. Karoglan Kontic Survey of major grapevine virus diseases in the vineyard of Valais (Switzerland) Besse S. Salmerón. E.A. Clair. F.. E. Martin Survey of wild grapes. X. I. P. Larreina. I. M. Hors Série – Extended abstracts 16th Meeting of ICVG. P.. -3. Lucas 104 106 108 110 112 114 116 Poster 25 Poster 26 Poster 27 Poster 28 118 120 122 124 Poster 29 Poster 30 Poster 31 Poster 32 126 127 129 131 Session V . A.V. Sim. Limanska. -4 and -6 in Spanish vine material according to different ecosystems.J.J. Aldamrat. geographical distribution and biological properties of two Flavescence dorée phytoplasma strains Salar P. Nancarrow. Marzachi’ Current status of grapevine viruses in the pacific northwest vineyards of the united states Rayapati N.A.. Terjék Emerging diseases of the grapevine at the Ukraine Milkus B. Cvjetkovic.F. Foissac Ecology and taxonomy of Flavescence dorée phytoplasmas: the contribution of genetic diversity studies 132 Molecular identification and geographic distribution of Flavescence dorée phytoplasma strains Bertaccini A. B. Angelini Aspects of the interaction of Stolbur phytoplasma. E.. Dermic. K. E. Zhun'ko. B. Osman. N. Boudon-Padieu. Caciagli. El Sayar. weed and cover crop species for grapevine viruses Golino D.. Dijon.A. Kamali. Recio. Foissac. M. García de Rosa. 2009. Carle. I. Cabaleiro Poster 23 Poster 24 A preliminary survey of grapevine viruses in kurdistan province (West of Iran) Afsharifar A. Tonon.. Klaassen..D. Malembic-Maher Genetic polymorphism of stolbur phytoplasma in grapevine. S.. Rodoni Frequence of occurrence of grapevine virus disease-complexes based on over 30 years indexing-results in Hungary Lázár J. Ioannou Presence of GLRaV -1. Duduk Comparison of prevalence. Tefera. R.. Konup Sanitary status of authochthonous minor wine grape varieties: an unusual diffusion of Arabis mosaic virus Materazzi A. Sykas. R. Forte. Seitz. Pejic. Maletic. A. Gugerli A survey of grapevine viruses in native cultivars in old plantations of tefneti – Arge vineyard. Gandhi. B.. Harighi. D. C. F. Hita. Sridhar..Session IV – Epidemiology – Survey of vineyards Oral Development and validation of sampling strategies for the detection of endemic viruses of australian grapevines Constable F. Legorburu F. E. C. wild plants and insects Filippin L.. Salar. Rowhani Contrasting epidemiologies of grapevine viruses depending on appellation and variety. I. K. Murolo.. Bianco Grapevine cultivar Plovdina – is it really an indicator plant for flavescence dorée disease ? Kuzmanovi S. Y. Paltrinieri. Hren. Bianco. A. S. Palmano Study of rplP gene for characterization and phylogenetic analysis of phytoplasma strains within the 16Sr-V group Durante G. Clair. R.. Mesquita. P. A. L. Sharon. M. A. P. cv. C.. Romanazzi Poster 33 Proteomic analysis of grapevine (Vitis vinifera L. Casati. Marzachi’ 145 147 149 151 153 155 Poster 34 157 Poster 35 Poster 36 159 161 Poster 37 Poster 38 Poster 39 Poster 40 Poster 41 162 164 166 168 170 Poster 42 Poster 43 Poster 44 172 174 176 Poster 45 Poster 46 Poster 47 Poster 48 Poster 49 178 180 182 184 186 Poster 50 188 . A. G.. Duduk. G. C. P. P. J. Toši Phytoplasma diffusion through grapevine propagation material and hot water treatment Mannini F. Filippin.A... Angelini High occurrence of Scaphoideus titanus on wild rootstocks in North Eastern Italy Forte V. Ravnikar. Nebbiolo) midrib tissues infected by flavescence dorée phytoplasma Margaria P. P. A. P. I. Terrier. P. Poggi Pollini. A. L. J.. Mawassi. L.. F. Casati. Sá. Daffonchio.E. Y. S.. Pisi. D. P. La Rosa. Durante. D. Ivanovi. J. P. Borgo. Ratti. Stojanovi.R. C.. Veratti. Quaglino. Angelini Spatial correlation of Scaphoideus titanus Ball adults on European grapevine at a plot scale: a case study Lessio F. yellows infected and recovered grapevines Bulgari D. Oliveri. Mollo Towards strain differentiation among grapevine bois noir phytoplasmas Contaldo N. R. Borgo. Argamante. C. Cardoso. Naor ‘Bois noir’ phytoplasma induces significant reprogramming of genes involved in carbohydrate metabolism and photosynthesis in the field grown grapevine Dermastia M.. S. Bianco Real-time PCR assay for diagnosis of Flavescence dorée (16Sr-V) Durante G. Rotter. D. V. R. Bertaccini Genetic diversity among Bois noir phytoplasma populations in Italy: new 16Sr subgroups and distinct SNP genetic lineages Quaglino F. Bertaccini Epidemics of Flavescence dorée disease in Portugal Sousa E. Cavalieri.. Oliveira.affected grapevines Landi L. C. P. W. M. Marzachi’. M.E. E. M. B.. W. Loi. Colombo. M.. Silletti.. Ember. F.A. A. Ermacora. Burger Biochemical pathways in phytoplasma–plant interactions in symptomatic and recovered leaves of Bois noir .. C... G. P.. Bosco Graft transmission trials of Flavescence dorée phytoplasma from recovered grapevines Carraro L. D.. Bianco. Borgogno Mondino. N. V. Joši.T. Wei. C. E. A. P. M. K. G. P.A. Alma Do Scaphoideus titanus larvae aggregate for feeding ? Chuche J. Kölber. M. Starovi. Gambino. Nikoli. Casati. D. Quaglino. N. Casati. Gruden Detection and identification of a new phytoplasma disease in grapevines in the Western Cape Engelbrecht M. Casati Detection of grapevine Flavescence dorée and Bois noir phytoplasmas by multiplex Real-time PCR (Taqman®) Terlizzi F. Boursault. Joubert. Brusetti. Casati.A. N. Credi Detection of phytoplasmas associated with grapevine yellows in rootstocks Borgo M. Paltrinieri.A. Casati. J... Savino. Romanazzi. P. Bertazzon. E. Zhao. Thiéry Acquisition of Flavescence dorée phytoplasma by Scaphoideus titanus Ball from recovered and infected grapevines of Barbera and Nebbiolo cultivars Galetto L. Davis Long term effects of Stolbur phytoplasma on grapevines in Israel Zahavi T. Dalla Cia. V. Bianco Flavescence dorée in the Empordà (Catalonia): results of Scaphoideus titanus containment through aerial treatments Torres E. Ž. Bech. S. G. N. Zhao. A. M. S. E.. Alma. Durante. Calvi. Boudon-Padieu. Martini. Osler Microbial diversity in healthy. Murolo. Bianco.. A. F. Rahola. G. M. P. R. Wei. M. F. Romanazzi. R. R. Roggia.Molecular markers among stolbur phytoplasma (16SrXII-A) strains and their association with natural ecologies of grapevine Bois noir in Italy Quaglino F. M.A. S. M. G. Aleksi. Davis Preliminary results on the variability of Bois noir isolates in a vineyard system in Sicily Tessitori M. Habili. Auchenorrhyncha.T. P.. I. R. F. D.T. Stephan. Du Preez. Altmann. E. Isidoro. Mikec Identification of stolbur-related phytoplasmas in grapevine showing decline symptoms in Iran Karimi M. Mély. Herranz-Gordo. C. E. B. O. P. Mawassi. Vigne. A. N. Neri Abiotic stress and treatment with elicitors for the control of Bois noir in Chardonnay vineyards Romanazzi G.. Valat. E. A. L. M. Golino. vinifera cultivars and application of VIGs in the cv. Burger Grapevine fanleaf virus replication: illuminating the way Schmitt-Keichinger C. Burgyan High-Throughput sequencing analysis of RNAs from a grapevine showing Syrah decline symptoms reveals a multiple virus infection that includes a novel virus Al Rwahnih M. Giampetruzzi. M. J.. impact on GFLV diversity and exchanges between rootstock and scion Hemmer C. V.. D’Ascenzo. A. Burger 214 216 218 220 222 224 226 Session VII . D.A. R..J Maree. Sabaté. Masciulli. G.J.G. Y.. Mahmoudi. 31 Aug – 4 Sept 2009 . T. Garau Reaction of different grapevine varieties to summer pruning measures for combating Bois noir Ipach U.A. J. Rowhani Characterisation of the genomic and subgenomic RNA of Grapevine leafroll-associated virus 3 (GLRaV-3) Maree H. Chapuis. Sela A universal vector for expression and silencing in plants (including grapevine) 212 Expression/silencing of GVA components in grapevine and N. G. Sultana Stephan D. Dijon. M-C.J.. Celton. Stander. Demangeat. Zorloni. Agalliinae) transmits a stolbur type phytoplasma Riedle-Bauer M. Sela. S. J. Zanini. L. B.A. Prosser 190 Poster 52 Poster 53 Poster 54 Poster 55 Poster 56 193 195 198 200 201 Poster 57 202 Poster 58 204 Poster 59 Poster 60 Poster 61 206 208 210 Session VI – Molecular biology – New technologies Oral Invited lecture Peretz Y. Jooste.. Helmstätter Can pruning measures combat bois noir disease of grapevine ? Riedle-Bauer M. I.A.. Muruganantham. Euscelis lineolatus. Tanne. V.. V. G. Mozes-Koch. S. E. N.. B. Komar. D. J. Burger Deep sequencing analysis of viral short RNAs from Pinot noir clone ENTAV 115 Pantaleo V. M. S. Budinšak. 1938) (Hemiptera. Neoaliturus fenestratus and Psammotettix alienus captured in vineyard agro-ecosystems in the Marche region (Central-Eastern Italy) Landi L. J. Ž. Murolo.Poster 51 Incidence of Bois noir phytoplasma in different viticulture regions of Spain and stolbur isolates distribution in plants and vectors Batlle A. F.. Riolo Further data on occurrence of grapevine yellows-associated phytoplasmas in vineyards of Veneto region (north-eastern italy) Quaglino F. A. J. D. Freeborough. E. D. N. Škori. benthamiana using the IL-60 system Peretz Y. 2009. K. Duduk. Daubert. J-M. Sára Vector–phytoplasma relationships during natural infection of Hyalesthes obsoletus. N. P. Gisel. Elamawi. Moxon. Miozzi. Tanne Virome of a vineyard: Ultra deep sequence analysis of diseased grapevines Coetzee B. S. Hors Série – Extended abstracts 16th Meeting of ICVG. Laviña Photosynthesis and transpiration in grapevines affected by Bois noir and recovered Murolo S... Regner.E. R. Goldschmidt. Müller. A. S. A. Dalmay. J. Kling. I. Contaldo.. M-J. Bianco Survey of phytoplasma diversity in heavily grapevine yellows affected areas of Croatia Šeruga Musi M.D. A.. Marmonier. Mori.. P.Virus effects – Control – Crop performances Oral Transgenic rootstocks expressing GFLV coat protein gene in a three years field trial. W. Ritzenthaler Poster 62 Vacuum-agroinfiltration of different V. Rees. L.T. Lemaire 228 Progrès Agricole et Viticole.. A. Freeborough.. C. Hanak. resistance assessment. I. J. J.. Vigneron. M-J. Prota. Tiefenbrunner Anaceratagallia ribauti (Oss. A. Vivier. Casati. D. Bertaccini New high incidence of Australian grapevine yellows and evidence on pathogenesis following natural heat therapy in severely infected South Australian vineyards Magarey P.. P. Courte. Masson. H. Didier. Romanazzi. Križanac. Saldarelli. France. Rowhani Virus effects on vine growth and fruit components of Cabernet Sauvignon on six rootstocks Golino D.. R. Karadag. A. G. C. Komínková Effect of Grapevine fanleaf virus (GFLV) and Grapevine leafroll-associated virus 3 (GLRaV-3) on red wine quality Legorburu F. Spreeth.F.C. Aguirrezábal Leafroll symptoms caused by GLRaV-3 are associated with an increase of resveratrol in grapevine leaves Bertazzon N.A.. M. E. A. A. Wolpert. P. N. Eleftheriou. Randles Poster 63 Detection and elimination of grapevine fanleaf virus in callus. Oosthuizen. T.. Minafra. Decarli. De Rosso. E... L. H. E. N.. J. Kaya. E.P. Moscon Control of grape berry mechanical properties modifications due to GFLV by mean of texture analysis Santini D. Lottering. N.A case study of control of grapevine leafroll disease spread on Vergelegen wine estate. Vallania. somatic embryos and regenerated plantlets of grapevine Gambino G. South africa. Buciumeanu. M.M. Bianchedi. Cuozzo. I.T. Mollo. E.E. J. G. M.. Rolle. P. P. Anderson. V. Zulini. Moscon. Nicolini Effects of GLRaV .. Gribaudo Virus elimination in infected clones of four Greek grapevine wine cultivars (Roditis. Larreina.) under the influence of various virus infections Gu I. Martinson. J. Wolpert. E-C. Triolo Advances on the eradication of Grapevine rupestris stem pitting associated virus (GRSPaV) from Vitis vinifera explants Skiada F. Suberviola. T... Recio. S. Ben-Dov Distribution and diversity of Leafroll-associated ampeloviruses in viruliferous vectors and grapevine hosts Fuchs M. A. Loeb. G.1 elimination on physiological. Decarli. agronomic and oenological characteristics of two cv. Hoch 264 269 271 .I. A. Gugerli Field performances and wine quality modification in a clone of Dolcetto (Vitis vinifera l. R.. Cameron. J. 20022008 Pietersen G. V. Rossouw Tentative analysis of the economic impact of grapevine leafroll disease in the vineyard of Valais (Switzerland) Besse S. Bianchedi. A. Nicolini. J. Sim. O. Vecchione. S. R. Rütsche. Baigorri. Remesal.. Cibriaín.. A. vinifera Marzemino clone Malossini U. vinifera l. Oztekin. Rowhani Comparative study of in vitro behaviour of grapevine (V. Y. L.) after GLRaV-3 elimination Mannini F. A. Mannini 2-amino-6-mercaptopurine: a preliminary study of a novel chemical group for grapevine antiviral therapy Luvisi A. Feteasc neagr cv. E. Panattoni. Caminero. Borgo Effects of gva elimination on physiological. Baftra and savatIano) using in vitro thermotherapy and tip meristem culture Grammatikaki G.. agronomic and oenological characteristics of a V. D. S. D. E. Sim.. Repetto. Zulini.. J.C. Current advances in the epidemiology of Grapevine leafroll disease First epidemiological study of ampeloviruses in Turkish vineyards Buzkan N. D.. Vecchione. Xinomavro. Marzemino clones Malossini U. López. F. L. Tooth. R.A. Flamini. Avgelis Virus effects on vine growth and fruit components of three California ‘heritage’ clones of Cabernet Sauvignon Golino D. Maliogka. F. Anderson.I. A. Benz. M. Angelini. A. Marsella-Herrick.J. M. R. Katis 230 232 234 237 239 Poster 64 241 Poster 65 243 Poster 66 Poster 67 245 247 Poster 68 Poster 69 249 251 Poster 70 Poster 71 252 254 Poster 72 256 Poster 73 Poster 74 Poster 75 258 260 262 Session VIII Viruses of the leafroll complex Oral Invited lecture Cabaleiro C. L. A. E. I. Benz. Van Rensburg. M.T... P. J. Vioiu A study on LN33 grapevine infected with five viruses Komínek P. J. Credi A mild strain of Grapevine leafroll-associated virus 3 is present in desirable clones of Crimson seedless table grapes in western australia Habili N. France. Herrbach. Hors Série – Extended abstracts 16th Meeting of ICVG. Douglas Monitoring of Grapevine leafroll-associated virus 1 (GLRaV-1) dispersion by the mealybug Phenacoccus aceris Le Maguet J. M. Burger Taxonomy.. W. Teixeira Santos. Beuve. 5.. 31 Aug – 4 Sept 2009 . 7 and 9 Besse S.Identification and distribution of three divergent molecular variants of Grapevine leafroll-associated virus 3 (GLRaV-3) in South african vineyards Jooste E. I.. V. G. C.P. J. Turturo. Goszczynski.R. Cohen. O. Hommay. Pearson. S. Eiras-Dias. Rowhani Sequence variation in Grapevine leafroll-associated virus-3 (GLRaV-3) New Zealand isolates Chooi K.. C. Gómez Talquenca 296 299 Poster 79 Poster 80 Poster 81 Poster 82 Poster 83 Poster 84 302 304 306 308 310 Poster 85 312 Poster 86 314 Progrès Agricole et Viticole. L. I.E.T. J. Jelkmann Comparison of phylogenetic inference based on HSP70. De Lurdes Rocha. Gugerli Serological and molecular characterisation of Grapevine leafroll-associated virus 2 variants occurring in Switzerland Besse S.E. Boudon-Padieu. C-W. Grenan Partial molecular characterization of Grapevine leafroll associated virus-1 isolate from Iran Roumi V. W.P. W. 2 and 3 on grapevine rootstocks varieties Beccavin I. M. A. Muñoz Detection of leafroll GLRaV-1. M.. Repetto. 2009.7 Mikona C.E.R. M.H. 6. M. D... Herrbach Rapid spread of leafroll disease in Cabernet Sauvignon grapevines in Napa Valley. De Lurdes Rocha. Krüger Advances in molecular detection of GLRaV-1 Bertazzon N. Lemaire Transmission trials of grapevine viruses by the mealybug Planococcus ficus (Hemiptera: Pseudococcidae) Credi R. Fonseca Genetic variability of Grapevine leafroll-associated virus 2 (GLRaV-2) in a Portuguese vineyard. Fonseca RFLP analysis of RT-PCR products to evaluate the genetic variability of CP ORF in GLRaV-2 isolates Lanza Volpe M. Komar... Fonseca The role of seasonality on mealybug transmission of Grapevine leafroll-associated viruses: an ecological hypothesis Almeida R.. F.. J. Lemaire. C. A. Teixeira Santos. Pietersen . M. E.P. J. Martini. I. E.. F.. HSP90 and CP genes of GLRaV-3: implications for detection and taxonomy Teixeira Santos M. Esteves. Gribaudo Transmission of Grapevine leafroll-associated virus 3 (GLRaV-3) by three soft scale insect species (Hemiptera: Coccidae) and notes on their developmental biology on grapevine Krüger K. D. Pong Poster 76 A real-time RT-PCR assay for the detection and quantification of Grapevine leafroll-associated virus 3 (GLRaV-3) in Vitis vinifera l.. E. Bitterlin. K.E. Cortez Serological relationships between grapevine infecting ampelovirus from Argentina Gómez Talquenca S. J. Daane Spread of GVA. G. G. Izadpanah Genetic diversity of Portuguese isolates of Grapevine leafroll-associated virus 1 (GLraV-1) based on the capsid protein Esteves F... O. (Vitaceae) and Planococcus ficus (Signoret) (Hemiptera: Pseudococcidae) Douglas N.. N. F. De Lurdes Rocha. O. Lemaire. M. Dijon. J. Bosco. Le Maguet. G. E. J. K. Hofstetter. Afsharifar. Lanza Volpe.M.A. O. G. F. based on the HSP70h and capsid protein genes Esteves F.T. A. M. F. Borgo. Martelli. Cuozzo. Terlizzi. complete nucleotide sequence and genome organization of grapevine leafroll-associated virus . M. Gambino.N.M. Balmelli.. Bazzo. Angelini 273 275 276 278 279 281 283 285 286 288 290 292 Poster 77 Poster 78 294 Development of an ELISA for the simultaneous detection of Grapevine leafroll-associated virus 4. Mannini.C.M. M. Pan. Minafra. H.E. D. Eiras-Dias. S. Beuve. California Golino D. Sim. S. Eiras-Dias.. Menzel. Pietersen. E. D. P. Gugerli Serological detection of Grapevine leafroll associated viruses 1. V. Tsai.. F. K. B. Maree . S. M.. O. Borsari Transmission of Grapevine leaf roll-associated virus-1 and -3 (Ampelovirus) and Grapevine virus A (Vitivirus) by natural populations of soft scales and mealybugs in the North-Eastern French vineyard Hommay G. Navarro. P.J. Weber. Rott. A.. GLRaV-1 and -3 and role of the mealybug vector Heliococcus bohemicus in a vineyard of Langhe (Northwestern Italy) Bertin S. 2 and 3 Cabaleiro C. Gracia. Pesqueira. G. D. M. G. De Biasi.E. Materazzi. Izadpanah 346 Poster 93 Poster 94 Poster 95 Poster 96 348 350 352 354 Author index 356 . C. D.. Nolasco Grapevine leafroll disease in Vitis vinifera cv Mencía Pesqueira A. Panattoni. Martelli Acquisition and transmission of GLRaV-1 (Grapevine leafroll associated ampelovirus 1) by Planococcus citri (Risso) (Hemiptera: Pseudococcidae) Scotto C. E. H. J. Li. B. S. C. Romanazzi. Haviv. B.. Burger Single domain antibodies for detection of GVB Masri S. E. V. L... Rast. Muruganantham. Cabaleiro Further data on the sensitivity of different rootstocks to the graft incompatibility associated with GLRaV-2 infection Pirolo C. M. Spilmont. Teixeira Santos. E. Della Bartola. serological and molecular characterization of Grapevine virus A isolates infecting vines in the Marche region. Moury. C.. Fonseca.T. F.. Burger Towards the elucidation of Grapevine virus A ORF 2 gene function Du Preez J.. Triolo 316 Poster 88 Poster 89 318 320 Poster 90 322 Session IX – Rugose wood complex Oral Invited lecture: Saldarelli P. Habili Poster 91 Biological. Angelini Syrah decline: no evidence for viroid etiology Renault-Spilmont A-S. La Notte. Goszczynski.T. D. A-S. Savino A novel vitivirus. Mochizuki. G. Anaclerio. Angelini Molecular characterization of biologically divergent strains of GRSPaV Bouyahia H. Nakano 324 327 329 331 333 336 338 341 Poster 92 344 Session X – Diseases of unclear etiology Oral Viral sanitary status and genetic diversity of Rupestris stem pitting associated virus in French Syrah clones exhibiting various susceptibility levels to decline Beuve M... A. Du Preez. N. Serra.. Hemmer. Sempé-Ignatovic. Wang Characterisation of ORF5 of three South African Grapevine virus A variants as pathogenicity determinant in Nicotiana benthamiana Blignaut M. Grapevine virus E Nakaune R. G. O. N.. González-Rodríguez. M. M. E. Oosthuizen. Boscia.Poster 87 Assessment of GLRaV-3 variants occurring in Portuguese grapevine varieties according to the coat protein gene Gouveia P. A. Bernard. Stephan. M. P. J. K. Filippin.. Eiras-Dias.. Mawassi. Flexiviruses: a grapevine point of view Different variants of GRSPaV are associated to diverse diseases in grapevine Borgo M. T. Lemaire Preliminary investigations on a Syrah decline in Central Italy Bianco P. Bertazzon. N.. Bertazzon. Zorloni. Triolo Toward development of Grapevine rupestris stem pitting-associated virus into a VIGS vector Meng B. D. Mawassi. P. Stephan. Jooste. Boscia. Belli Shiraz disease and grapevine yellows in South Africa Pietersen G.A. Cardone. R. A.. F.E. S. J.M. A. M. Esteves. M. J. F.. L. L.. N.P. Toda. D. Central-Eastern Italy Murolo S. W. Conti. Duran-Vila Identification and partial characterization of grapevine viroids in Southern Iran Zaki-Aghl M. E. C.