EBC

September 2006Analytica-EBC Dates of last amendments: new and revised methods Contents Method number 0 Safety 0.1 Safety in the Laboratory 09 / 1997 Title Dates of last amendments 1 Apparatus 1.1 1.2 1.3 1.4 1.5 1.6 2 Water 2.1 2.2 2.3 3 Barley 3.1 3.2 3.3.1 3.3.2 3.4 3.5.1 3.5.2 3.6.1 3.6.2 3.6.3 3.7 3.8.1 3.8.2 3.9 Sampling of Barley Moisture Content of Barley Total Nitrogen of Barley: Kjeldahl Method Total Nitrogen of Barley: Dumas Combustion Method Thousand Corn Weight of Barley Germinative Capacity of Barley: Rapid Staining Method Germinative Capacity of Barley: Hydrogen Peroxide and Peeling Method (RM) Germinative Energy of Barley: Aubry Method Germinative Energy of Barley: BRF Method Germinative Energy of Barley: Schönfeld Method Germinative Percentage and Germinative Indices of Barley Pre-Germinated Grains in Barley: Fluorescein Dibutyrate Method Archived Husk Content of Barley 10 / 2004 09 / 1997 10 / 2004 10 / 2004 09 / 1997 06 / 2005 10 / 2004 09 / 1997 09 / 1997 09 / 1997 09 / 1997 09 / 2006 09 / 1997 Sampling of Water Chloride in Water: Conductometric Method Sulphate in Water: Gravimetric Method 09 / 1997 09 / 1997 09 / 1997 Care and Adjustment of Apparatus: Bühler Miag Disc Mill, DLFU Care and Adjustment of Apparatus: Spectrophotometers Care and Adjustment of Apparatus: Atomic Absorption Spectrophotometers Care and Adjustment of Apparatus: Gas Chromatographs Care and Adjustment of Apparatus: pH Meters Care and Adjustment of Apparatus: Density Meters 09 / 1997 09 / 1997 09 / 1997 in preparation 05 / 1999 05 / 1999 1 1 4. Final Attenuation of Laboratory Wort from Malt (RM) Fermentability.9. Lager and Distilling Malts: Constant Temperature Mash Diastatic Power of Malt α-Amylase Content of Malt (IM) Modification and Homogeneity of Malt: Calcofluor Method Friability.13 Title Archived High Molecular Weight β-Glucan Content of Barley: Fluorimetric Method Sieving Test for Barley Visual Examination of Damaged Barley Kernels Identification of Varieties in Barley Moisture and Total Nitrogen in Barley by Near Infrared Spectroscopy Dates of last amendments 10 / 2004 12 / 2002 10 / 2004 12 / 2002 09 / 1997 4 Malt 4.3 4.3.12 4.17 Sampling of Malt Moisture Content of Malt Total Nitrogen of Malt: Kjeldahl Method Total Nitrogen of Malt: Dumas Combustion Method Thousand Corn Weight of Malt Extract of Malt: Congress Mash Archived Hot Water Extract of Malt: Constant Temperature Mash Cold Water Extract of Ale.3 4.September 2006 Method number 3.1 4.2 3.8 4.2 3.6.7.2 4.2 4.1 3.11. Final Attenuation of Unboiled Worts of Ale.2 4. Final Attenuation of Laboratory Wort from Malt (Rapid Method) Fermentability.3 4.16.1 4.2 4.11.9.2 4.6 4.1 3.12 3.5.13 4.5. Lager and Distilling Malts Colour of Malt: Spectrophotometric Method (IM)(RM) Colour of Malt: Visual Method Viscosity of Laboratory Wort from Malt Soluble Nitrogen of Malt: Kjeldahl Method Soluble Nitrogen of Malt: Spectrophotometric Method Soluble Nitrogen of Malt: Dumas Combustion Method Free Amino Nitrogen of Malt by Spectrophotometry Fermentability.2 4.16.11.4 4.9.7.11.1 4. Glassy Corns and Unmodified Grains of Malt by Friabilimeter (IM) Archived High Molecular Weight β-Glucan Content of Malt: Fluorimetric Method High Molecular Weight β-Glucan Content of Malt Wort: Spectrophotometric Method Moisture and Total Nitrogen in Malt by Near Infrared Spectroscopy 09 / 1997 10 / 2000 10 / 2004 10 / 2004 09 / 1997 10 / 2004 09 / 1997 10 / 2004 10 / 2000 09 / 1997 10 / 2004 09 / 1997 09 / 1997 05 / 1999 09 / 1997 09 / 1997 05 / 1999 10 / 2004 09 / 2006 09 / 2006 06 / 2005 09 / 1997 10 / 2004 06 / 2005 09 / 1997 2 .10.2 4.11.3.14 4.1 4.15 4.10.10 4.2 4.1 4.16.1 4. 1.18 4. Syrups and Caramel 6.12 6.1 6. Barley Flours.4 5.2 6.1 6.2.19 4.1 5.22 Title Archived Boiled Wort Colour Identification of Barley Varieties in Malt Glycosidic Nitrile in Ale.5 5. Gelatinised Flakes and Torrefied Products: Constant Temperature Mash Hot Water Extract of Untreated Grain Grits and other Cereal Raw Grain: Constant Temperature Mash Colour of Wheat Flours.6 5.10 6.5 6.1.15 6.10 Sampling of Coloured Malts and Coloured Malt Products Coloured Malt Beer: Extract Coloured Malt Beer: Colour Coloured Malts: Moisture Coloured Malts: Extract Coloured Malts: Colour: Visual Method Coloured Malts: Constant Temperature Mash: Extract Coloured Malts: Constant Temperature Mash: Colour: Visual Method Dark Malts and Roasted Barley: Constant Temperature Mash: Extract Dark Malts and Roasted Barley: Constant Temperature Mash: Colour: Visual Method 09 / 1997 09 / 1997 09 / 1997 10 / 2000 10 / 2000 10 / 2000 10 / 2004 10 / 2004 10 / 2004 10 / 2004 6 Cereal Adjuncts.20 4.3 5.8 5.2.7 6.16 Sampling of Cereal Adjuncts Sampling of Sugars and Syrups Moisture Content of Unmalted Cereal Adjuncts other than Maize Moisture Content of Maize Extract Content of Solid Adjuncts: ASBC Method (IM) Extract Content of Solid Adjuncts: De Clerck Method Extract Content of Maize: Enzymatic Method Extract Content of Liquid Adjuncts: ASBC Method (IM) Extract Content of Caramel Colour of Caramel Colour of Liquid Adjuncts (IM) Fatty Substances in Cereal Adjuncts Archived Iron in Sugars and Syrups Copper in Sugars and Syrups Hot Water Extract of Wheat Flours.September 2006 Method number 4.9 5.21 4.13 6.11 6. Gelatinised Flakes and Torrefied Products: Constant Temperature Mash: Visual Method 10 / 2004 09 / 1997 10 / 2004 09 / 1997 10 / 2004 10 / 2004 10 / 2004 10 / 2004 09 / 1997 10 / 2004 10 / 2004 09 / 1997 10 / 2004 10 / 2004 10 / 2004 10 / 2004 10 / 2004 3 .8 6.7 5.4 6.9 6.2 5.3 6. Lager and Distilling Malts Sieving Test for Malt Dates of last amendments 10 / 2000 12 / 2002 09 / 2006 06 / 2005 5 Coloured Malts and Coloured Malt Products 5.6 6. Barley Flours.2 6. Sugars.14 6. Powders and Pellets Bitter Substances in Hops and Hop Products: Lead Conductance Value and Total Resin.12 * Sampling of Hops and Hop Products Moisture Content of Hops and Hop Products Seed Content of Hops Lead Conductance Value of Hops.19 Sampling of Wort Specific Gravity of Wort using a Pyknometer Specific Gravity of Wort using a Density Meter Extract of Wort Viscosity of Wort Colour of Wort: Spectrophotometric Method (IM) Fermentability.9 7.11 8. α.10 8.13.7 7.1 8.2 8.2 7.1 8.2.2 8. Soft Resin and Hard Resin α.and β-Acids in Hops and Hop Products by HPLC Iso-α-.2 8.6 7.and β-Acids in Isomerised Hop Pellets by HPLC Hop Essential Oils by Capillary Gas Chromatography Flame Ionization Detection 10 / 2004 09 / 1997 09 / 1997 10 / 2000 12 / 2002 12 / 2002 06 / 2005 06 / 2005 10 / 2004 12 / 2002 06 / 2005 09 / 2006 8 Wort 8. Attenuation Limit of Wort (RM) Fermentability.17 8.14 8.10 7.3 8.6.12 8.7 8.1 8.16 8.18 8.5 7.8 7.1 7.and β-Acids in Hop and Isomerised Hop Extracts by HPLC Iso-α-Acids and Reduced iso-α-Acids in Hop Products by HPLC Hop Oil Content of Hops and Hop Products Iso-α.1 8.9.6.1 8.2.4 7.9.3 7. Soft Resin and Hard Resin Bitter Substances in Hop Extracts: Lead Conductance Value and Total Resin.13.2 8.11 7.15 8. Attenuation Limit of Wort (Rapid Method) Fermentable Carbohydrates in Wort by HPLC Bitterness of Wort Total Nitrogen in Wort: Kjeldahl Method Total Nitrogen in Wort: Dumas Combustion Method Free Amino Nitrogen in Wort by Spectrophotometry (IM) Zinc in Wort by Atomic Absorption Spectrophotometry (IM) Total Polyphenols in Wort by Spectrophotometry Archived High Molecular Weight β-Glucan Content of Wort: Fluorimetric Method Archived Archived Archived pH of Wort Calcium in Wort by Atomic Absorption Spectrophotometry Dissolved Oxygen in Wort using Electrochemical Sensors 05 / 1999 10 / 2004 10 / 2004 10 / 2004 10 / 2004 10 / 2000 12 / 2002 12 / 2002 10 / 2004 10 / 2004 10 / 2000 05 / 1999 12 / 2002 10 / 2004 09 / 1997 10 / 2004 05 / 1999 10 / 2004 10 / 2004 4 .8 8.4 8. α.September 2006 Method number Title Dates of last amendments 7 Hops and Hop Products 7.5 8. 11 9.2 9.2 9.2 9.26 Sampling of Beer before Filling Alcohol in Beer by Distillation Alcohol in Beer by Catalytic Combustion Alcohol in Beer by Refractometry Ethanol in Beer by Gas Chromatography Correction of Volatile Acidity Ethanol in Alcohol-Free and Low Alcohol Beers: Enzymatic Method Original.2.18 9.14.8 9.3 9.14.2-Bipyridyl or 1.3 9.3 9.14.5 9.4 9.1 9.20 9.2.7 9.13.2.2 9.24.1 9.12 9.5 9.4 9.13.2 9.2. Real and Apparent Extract and Original Gravity of Beer Real Degree of Fermentation of Beer Colour of Beer: Spectrophotometric Method (IM) Final Attenuation of Beer Bitterness of Beer (IM) Total Nitrogen in Beer: Kjeldahl Method Total Nitrogen in Beer: Dumas Combustion Method Free Amino Nitrogen in Beer by Spectrophotometry (IM) Total Polyphenols in Beer by Spectrophotometry Flavanoids in Beer by Spectrophotometry Iron in Beer by Spectrophotometry with 2.25.2.September 2006 Method number 9 Beer 9.1 9.24.23 9.1 9.10 9.22 9.25.16 9.21 9.9.25.13.3 9.1 9.1 9.2 9.9.6 9.3 9.10Phenanthroline (IM) Iron in Beer by Spectrophotometry with Ferrozine (IM) Iron in Beer by Atomic Absorption Spectrophotometry (IM) Copper in Beer by Spectrophotometry with ZDBT (IM) Copper in Beer by Spectrophotometry with Cuprethol: Rapid Method (IM) Copper in Beer by Atomic Absorption Spectrophotometry (IM) Nickel in Beer by Spectrophotometry with Dimethylglyoxime Sodium in Beer by Atomic Absorption Spectrophotometry (IM) Potassium in Beer by Atomic Absorption Spectrophotometry (IM) Magnesium in Beer by Atomic Absorption Spectrophotometry (IM) Calcium in Beer by Atomic Absorption Spectrophotometry (IM) Zinc in Beer by Atomic Absorption Spectrophotometry (IM) Archived Archived Archived Vicinal Diketones in Beer: Spectrophotometric Method Vicinal Diketones in Beer: Gas Chromatographic Method Total Sulphur Dioxide in Beer: Distillation Method Archived Total Sulphur Dioxide in Beer: p-Rosaniline Method (IM) Total Carbohydrate in Beer by Spectrophotometry 12 / 2002 10 / 2004 09 / 1997 09 / 1997 09 / 1997 10 / 2004 09 / 1997 10 / 2004 09 / 1997 10 / 2000 10 / 2000 10 / 2004 10 / 2000 05 / 1999 10 / 2000 12 / 2002 09 / 1997 09 / 1997 09 / 1997 09 / 1997 09 / 1997 09 / 1997 09 / 1997 09 / 1997 09 / 1997 09 / 1997 09 / 1997 12 / 2002 09 / 1997 Title Dates of last amendments 10 / 2000 05 / 1999 09 / 1997 06 / 2005 10 / 2000 5 .1 9.19 9.15 9.17 9. 8 10.2 Sampling of Bottles Archived Filling: Air in Headspace of Bottles and Cans (Piercing Method) 10 / 2004 09 / 1997 6 .1 10.43.37 9.1 9.7 10.1 11. Sulphate.2 10.31.5 10.2 9.33 9.27 9.1 9.1.2 9.9 10.6 10.1 11.29 9.28.43.32 9.2.38 9.28.40 9.41 9. Nitrate and Phosphate (IM) Dissolved Oxygen in Beer by Electrochemical Sensors Viscosity of Beer: Glass Capillary Viscometer (IM) Dimethyl Sulphide and Other Lower Boiling Point Volatile Compounds in Beer by Gas Chromatography Sensitive Proteins in Beer by Nephelometry Alcohol Chill Haze in Beer (Test Chapon) Foam Stability of Beer using the NIBEM-T Meter Specific Gravity of Beer using a Pyknometer Specific Gravity of Beer using a Density Meter Lead in Beer by Atomic Absorption Spectrophotometry Energy Value of Beer by Calculation Dates of last amendments 09 / 1997 09 / 1997 09 / 1997 09 / 1997 09 / 1997 09 / 1997 09 / 1997 09 / 1997 10 / 2004 12 / 2002 12 / 2002 09 / 1997 10 / 2000 12 / 2002 10 / 2004 10 / 2004 10 / 2004 10 / 2004 10 / 2004 06 / 2005 10 Process Aids 10.10 10.11 Sampling of Solid Process Aids Moisture Content of Solid Process Aids Calcination Loss of Filter Aids Density of the Wet Filter Bed of Filter Aids pH of an Aqueous Suspension of Filter Aids (IM) Soluble Iron Content of Filter Aids (IM) Solid Brewing Materials: Effect on Aroma and Taste (IM) Permeability of Filter Aids (RM) Filtration Rate of Filter Aids (Routine Method) Density and Thickness of Filter Sheets Brewery Gases: Effect on Aroma and Taste 05 / 1999 06 / 2005 09 / 1997 09 / 1997 05 / 1999 09 / 1997 10 / 2004 09 / 1997 05 / 1999 09 / 1997 10 / 2004 11 Packaging and Packaging Materials 11.3 10.September 2006 Method number 9.36 9.31.39 9.4 10.2.1 9.34 9.2 9.42 9.30 9.44 9.35 9.45 Title Fermentable Carbohydrates in Beer by HPLC (IM) Carbon Dioxide in Beer: Titration Method (RM) Archived Haze in Beer: Calibration of Haze Meters Prediction of Shelf-Life of Beer Archived High Molecular Weight β-Glucan Content of Beer: Fluorimetric Method Acetic Acid in Beer: Enzymatic Method Glycerol in Beer: Enzymatic Method Lactic Acid in Beer: Enzymatic Method pH of Beer Anions in Beer by Ion Chromatography: Chloride. 17 * 11.25 * Title Process Control: Net Contents of Bottles and Cans (Weighing Method) Process Control: Net Contents of Bottles as Measuring Containers (Non Destructive Template Method) Process Control: Pasteurizing Units Total Oxygen in Packaged Beer Qualitative Assay for Invertase Phosphatase Test Empty One-Way Container Clean Efficiency Container Washer Detergent Analysis Detergent Residues in Washed Bottles Packaging Materials in Contact with Beer: Effect on Aroma and Taste of Beer Packaging Materials in Contact with Beer: Effect on Foam Characteristics Packaging Materials in Contact with Beer: Metal Pickup in Beer Visual Assessment (Defects List) of Glass Bottles Physical Dimensions of Bottles Capacity Determination of Bottles Internal Pressure Strength of Glass Bottles Glass Weight of Bottles Visual Assessment of Cans Overflow Capacity of Cans Headspace of Cans Internal Lacquer Integrity of Cans Can Seam Evaluation Capacity Determination of Kegs and Casks Coating Integrity of Crowns Electronic Bottle Inspection Devices (EBI) Checks for Secondary Packaging: Corrugated Board for Cases and Trays Dates of last amendments 09 / 1997 09 / 1997 09 / 1997 09 / 1997 10 / 2004 09 / 2006 10 / 2004 10 / 2004 10 / 2004 10 / 2004 09 / 2006 10 / 2004 10 / 2004 10 / 2004 10 / 2004 10 / 2004 06 / 2005 09 / 2006 09 / 2006 09 / 2006 09 / 2006 09 / 2006 09 / 2006 09 / 2006 09 / 2006 09 / 2006 12 Co-Products 12.3.11 11.3 Sensory Health and Safety Guidelines Sensory Analysis: Terms and Definitions (IM) Sensory Analysis: Test Room.2 * 11.18 * 11.September 2006 Method number 11.0 13.6.16 11.14 11.7 11. Equipment.1 11.2 13.10 11.4 11.12 11.9 11. Conduct of Test (IM) Sensory Analysis: Choice of Method (IM) 10 / 2004 09 / 1997 09 / 1997 09 / 1997 7 .22 * 11.6 11.5 11.2 * 11.23 * 11.24 * 11.4 Sampling of Spent Grains Moisture Content of Spent Grains Total Available Extract in Spent Grains Soluble Extract in Spent Grains 09 / 1997 09 / 1997 09 / 1997 09 / 1997 13 Sensory Analysis 13.2 12.20 * 11.21 * 11.8 11.13 11.2 11.19 * 11.1 13.15 11.3 12.10.3.1 12. 41 Plastic Tubing: Full Method (Taint Testing) 12 Co-products 12.2 14.10 13.7 Total Residual Extract in Spent Grains 13 Packaging 13.11 Light Transmission of Bottles 13.12 Visual Assessment of Crowns 13.1 14.35 Bottle Seal Inspection 13.36 Measurement of Total “Shake-out” Air 13.4 14.32 Bottle Seal Integrity Determination 13.5 External Lacquer Integrity of Cans 13.9 13. packaging and dispense equipment: effect on product quality 10.4 13.8 13.Packaging 01 / 1997 01 / 1997 01 / 1997 01 / 1997 01 / 1997 01 / 1997 01 / 1997 01 / 1997 01 / 1997 01 / 1997 01 / 1997 01 / 1997 01 / 1997 8 .5 Ruggedness Testing of Methods (IM) Collaborative Trial to Determine the Precision of a Measurement Method Trueness of a Standard Measurement Method Sampling Design Specification and Tolerance Bands 12 / 2002 12 / 2002 12 / 2002 12 / 2002 12 / 2002 15 IOB Methods awaiting harmonisation with EBC (IOB Method numbering) (Available from EBC Secretariat) 10 Brewing.7 Contamination of Can Ends 13.3 14.6 13.14 * Title Sensory Analysis: Selection and Training of Assessors (IM) Sensory Analysis: Author Guidelines for Reporting(IM) Sensory Analysis: Paired Comparison Test (IM) Sensory Analysis: Triangular Test (IM) Sensory Analysis: Duo Trio Test (IM) Sensory Analysis: Threshold of Added Substances (IM) Sensory Analysis: Description Analysis (IM) Sensory Analysis: Ranking Test (IM) Sensory Analysis: Flavour Terminology and Reference Standards (IM) Sensory Analysis: Routine Descriptive Test Guideline Routine Sensory Check of Raw Materials and In-process Samples Dates of last amendments 09 / 1997 09 / 1997 09 / 1997 09 / 1997 09 / 1997 09 / 1997 09 / 1997 09 / 1997 09 / 1997 10 / 2004 09 / 2006 14 Statistics 14.12 13.7 13.41 General Bibliography .13 13.28 On-line Fill Level Inspection Devices 13.30 Fill Level (Volume Control) .11 13.September 2006 Method number 13.34 Determination of Dissolved Oxygen in an Equilibrated (Shaken) Package 13.5 13.Keg and Cask 13. 1 7.1 11.5.19 5.4 7.6 7.16 9.22 9.11 7.2 4.1 Pre-germinated Grains in Barley: Methylene Blue Method (AM) High Molecular Weight β-Glucan Content of Barley: Enzymatic Method (AM) Extract Difference of Malt: Congress Mash (AM) High Molecular Weight β-Glucan Content of Malt: Enzymatic Method (AM) Malt: pH of Wort (AM) Apparent Fermentability of Sugars and Syrups (AM) High Molecular Weight β-Glucan Content of Wort Enzymatic Method (AM) Total Carbohydrate in Wort by Spectrophotometry (AM) Chloride in Wort: Conductometric Method (AM Sulphate in Wort: Gravimetric Method (AM) Chloride in Beer: Conductometric Method (AM) Sulphate in Beer: Gravimetric Method (AM) Nitrate in Beer: Enzymatic Method (AM) Total Sulphur Dioxide in Beer: Enzymatic Method (AM) Carbon Dioxide in Beer: Instrumental Method (AM) High Molecular Weight β-Glucan Content of Beer: Enzymatic Method (AM) Filling: Air in Headspace of Bottles and Cans (Funnel Method) (AM) 09 / 1997 09 / 1997 09 / 1997 09 / 1997 --- 09 / 1997 09 / 1997 09 / 1997 09 / 1997 09 / 1997 09 / 1997 09 / 1997 09 / 1997 09 / 1997 09 / 1997 09 / 1997 09 / 1997 17 Archived IOB Methods 2.4 7.21 9.16.23 9.4 5.2 7.12 Estimation of Endo-Beta-Glucanase Activity of Ale.13.1 8.3 7.2 9.11 8. by Refractometry.8.28.2 3.2 9.15 8.10 7.18 6.5 7.31.14 8.September 2006 Method number Title Dates of last amendments 16 Archived EBC Methods 3.1 4. of Foam Stabilisers: Propylene Glycol Alginate (AM) Alginic Content and Degree of Esterification of Foam Stabilisers: Propylene Glycol Alginate (AM) Determination of Optimum Concentration of Copper Finings (AM) Optimisation of the Performance of Isinglass Finings (AM) Free Sulphur Dioxide Content of Isinglass Finings (AM) 01 / 1997 01 / 1997 01 / 1997 01 / 1997 01 / 1997 01 / 1997 01 / 1997 01 / 1997 11 / 1997 11 / 1997 01 / 1997 01 / 1997 01 / 1997 9 . Lager and Distilling Malts (AM) Sulphated Ash in Sugars and Syrups (AM) Total Reducing Sugars of Sugars and Syrups (AM) Lead Conductance Value of Hops and Hop Products (AM) Dry Weight Content of Foam Stabilisers: Propylene Glycol Alginate (AM) Haze of Foam Stabilisers: Propylene Glycol Alginate (AM) Haze Potential of Foam Stabilisers in Beer: Propylene Glycol Alginate (AM) pH of Foam Stabilisers: Propylene Glycol Alginate (AM) Solids Content.2.25.1 4.5 6.10. 37 13. Note: AM IM RM Archived Methods are no longer standardised.21 10.14 13.23 13.29 13.33 13.36 9.21 13.T5) for Beer (AM) Saturated Ammonium Sulphate Precipitation Limit (SASPL) of Beer (AM) Head Retention of Beer: Rudin Method (AM) Hydrophobic Polypeptides in Beer (AM) Brewery Gases: Screening of Oil Contaminants (AM) Plastic Tubing: Screening Method (Taint Testing) (AM) Physical Dimensions of Cans (AM) Visual Assessment and Physical Dimensions of Can-Ends (AM) Visual Assessment of PET Bottles and Closures (AM) Oxygen Permeability of PET Bottles and Closures (AM) Bar Code Integrity (Examination) (AM) Quality Control of Kegs and Casks (AM) Quality Control of Kegs Extractors (AM) Checks for Secondary Packaging: Bottles.6 13.15 13. work on it was in progress when the IOB Analysis Committee was disbanded.40 * ** Title pH of Isinglass Finings (AM) Total Nitrogen.22 13.14 7.com) International Method Reference Method (Analytica-EBC-Contents 2006 + last amendment.33 9. Total Soluble Nitrogen and Soluble Collagen Nitrogen Content of Isinglass Finings (AM) Wet Sieve Analysis of Filter Aids (AM) Vicinal Diketones in Beer: Colorimetric Method (AM) Dimethyl Sulphide in Beer by Gas Chromatography (AM) Accelerated Ageing Test (T2.19 13.31 9. they can still be obtained from the EBC Secretariat (e-mail: [email protected] 13.doc) 10 .Bottles and Cans (AM) Internal Pressure in Cans and Bottles (AM) Measurement of Pasteurisation Units (PU’s) (AM) Package Presentation Checks (AM) Dates of last amendments 01 / 1997 11 / 1997 02 / 1999 01 / 1997 01 / 1997 01 / 1997 01 / 1997 11 / 1997 --01 / 1997 01 / 1997 01 / 1997 01 / 1997 01 / 1997 01 / 1997 01 / 1997 01 / 1997 01 / 1997 01 / 1997 01 / 1997 01 / 1997 01 / 1997 01 / 1997 01 / 1997 01 / 1997 New method This method has no number since it was never published in the IOB Methods of Analysis.16 13.41 ---** 10. Labels.September 2006 Method number 7.3 13.20 9.13 7.40 13. and Foils (AM) Checks for Secondary Packaging: Adhesives (AM) Check for Secondary Packaging: Plastic Crates for Bottles (AM) Off-line Fill Level (Volume Control) .39 9.