**Don’t forget to submit your pictures with your name and the date showing on apaper within the picture!** Lab 3: Biomolecules (15 points). Grading: Submission of data tables and results pictures with appropriate data and interpretation of each test result = 3 points. Part 1: Testing for Reducing Sugars Table 1: Experimental Results for Testing for Reduced Sugars Sample 1 – Potato 2 – Onion 3 – Glucose Solution 4 – Water 5 – Unknown Initial Color Light Brown Clear Clear Final Color Green Yellow Red Reduced Sugar Present? Y Y Y Clear Cloudy White Blue Green N Y Part 2: Testing for Starch Table 2: Experimental Results for Testing for Starch Sample 1 – Potato 2 – Onion 3 – Starch Solution 4 – Water 5 – Unknown Initial Color Light Brown Light Brown Cloudy White Clear Cloudy White Final Color Black Yellow Purple Light Brown Purple Starch Present? Y N Y N Y Part 3: Testing for Proteins Table 3: Experimental Results for Testing for Proteins Sample 1 – Albumin Solution 2 – Gelatin Solution 3 – Glucose Solution 4 – Water 5 – Unknown Initial Color Clear, slightly milky Light Yellow Clear Clear Cloudy White Questions to answer/submit: Final Color Purple Purple Light Blue Light Blue White Protein Present? Y Y N N N 1. For each type of molecule listed below, give on example of one of its functions in living organisms (3pts). a. Simple sugars (monosaccharide or disaccharide): They usually act as short term energy stores b. Starch: It is used for long term energy storage c. Protein: They act as defense, metabolism, support, as well as transport in cells 2. Which of the three types of molecules that you tested for does the unknown solution contain? How do you know? (2pts, answer both parts of this question) Simple sugars because it changed to green meaning the presence of a reducing sugar and starch because it changed to purple meaning starch is present. 3. What can you conclude about the molecular make-up of potatoes and onions based on the tests you performed? Why might these foods contain these substances? (2pts, answer both parts of this question) Potatoes and Onions both contain sugars. This is because they both are carbohydrates. 4. What results would you expect if you tested ribose, a monosaccharide, with Benedict’s solution? What results would you expect if you tested ribose with IKI? (2pts, answer both parts of this question) If ribose a monosaccharide were tested in Benedict’s solution I think it would result in a dark brown color, they both contain carbohydrates, so glucose will be present. All monosaccharides are reducing sugars. 5. Imagine I took a starch solution and I performed a reaction where hydrolysis occurred. When doing this test I first tested my solution and it was positive for starch and negative for reducing sugars such as glucose. How might my test results change after hydrolysis is complete? (hint: you need to know that starch is a large polymer and you need to know what monosaccharide makes up the starch polymer!) (2pts) Starch is a polysaccharide consisting of longer chains of glucose. After the hydrolysis of starch the longer chains will be broken down. The monosaccharides that will result are glucose. 6. The molecule pictured below produced a blue color when tested with Benedict’s reagent, a yellow color when tested with IKI, and a violet/purple color when tested with Biuret reagent. Based on the structure shown below and these chemical results, what kind of biomolecule is this? (1 pt) Protein