Abstract Book Tradmed Symposium.pdf

Track 01Interpretation of traditional historical manuscripts in light of modern science Abstracts of Keynote Speakers and Oral Presenters 1 Independence, Resurgence of Nationalism and the Contested Development of Traditional Medicine Dr. Rathnayake M. Abeyrathne Head/Department of Sociology, University of Peradeniya, Sri Lanka Traditional medicine has become a highly contested political issue in the aftermath of independence in Sri Lanka between pro-reformists and anti-reformists. In my presentation,I argue how a group of traditional medicine practitioners hindered the implementation of the recommendations put forwarded by the Das Gupta Report in 1947, and instead forced the government to appoint a committee to look into and promote Sinhala Medicine in the 1950s. It also argues how and why S.W.R.D. Bandaranayake incorporated traditional medicine themes into Sinhala Buddhist nationalist activities prior to coming to power in the 1956 elections. Furthermore, in this presentation, I will further elaborate on how pro-reformists and anti-reformists came to conflict over traditional medicine in Sri Lanka. Finally,in this presentation, I will debate that while the Sinhala Buddhist Nationalist movement was behind the growth of traditional medicine education, health care provision, and the registration of practitioners, much was not implemented. Politicians invoked traditional medicine to attract public support, but did not deliver on their promises. The tension between the Sinhala Buddhist Nationalists and the more inclusive nationalist discourses brought dynamism to the field of traditional medicine. It is therefore difficult for the historian of medicine to write a traditional medicine historiography of Ceylon without examining deeply the influence of language, religion, science, modernity and politics during this era. Keywords: Traditional Medicine, Independence, Resurgent of Nationalism, 2 Ayurgenomics for Exploring the Genomic and Molecular Basis of Translational Aspects of Ayurveda of Predictive and Personalized Medicine Dr. Bhavana Prasher CSIR Ayurgenomics Unit –TRISUTRA, CSIR- Institute of Genomics and Integrative Biology, Mathura Road, New Delhi, India. Traditional systems of Medicine follow a holistic approach to management of health wherein human beings are treated in totality taking into consideration their subtle relationship with the environment. Ayurveda, the Ancient Indian System of Medicine, which a living tradition of health and healing even to this day, deals with both wellness and illness in a predictive and personalized way. Some of its salient aspects include comprehensive documentation with regards tomedicinal preparations, clinical methods for diagnosis and assessment of variability in health and disease, for their personalized administration. It also advocates and provides methods for enhancement of knowledge including inter-disciplinary studies. (the sentence looks out of place here). According to Ayurveda, individuals are born with a specific proportion of tridosha which determines their ‘Prakriti’ or basic constitution. Prakriti to large extent determines an individual’s health trajectory, response to external environment including diet as well as susceptibility to diseases and their progression. “Tridosha” – vata, pitta and kapha, are physiological entities that work in conjunction with each other across systems and also respond to external environment to maintain homeostasis throughout lifetime. Their perturbation beyond threshold levels leads to disease manifestation and the goal of treatment is restore the balance to an individual’s baseline thresholds. The concept of Prakriti thus enables integration of baseline physiological variability with adaptability, disease manifestation and drug response. We at CSIR- TRISUTRA Ayurgenomics Unit are integrating the Prakriti methods with genomics and modern biology to understand their molecular and genomic basis. This we anticipate would enable identification of markers that would be pertinent for discovery and development of personalized medicine. Through this interdisciplinary framework of ‘Ayurgenomics’ we have been able to show molecular differences among normal healthy individuals of predominant Prakriti types. Hypoxia is one such axis that we have shown to differ between Pitta and Kapha Prakriti. Differences in environmental and cellular responses to hypoxia can influence adaptation and adaptability as well as outcomes of several diseases where hypoxia is either cause or consequence. These observations also highlight the need for personalized management of an individual in hypoxia condition. In this interactive session of Ayurgenomics we would share our group’s experience in understanding of Tridosha through Prakriti, finding modern biological basis of the concept how these insights are useful and applied to personalized medicine and Ayurveda based drug research. Keywords: Ayurgenomics, Traditional systems of Medicine, Tridosha, Prakriti 3 Sri Lankan Traditional Practices in Panchakarma with Special Reference to Vamana Karma using Lindernia pusilla (Wilandavenna) Prof. (Mrs.) E. R. H. S. S. Ediriweera Professor, Department of NidanaChikithsa, Institute of Indigenous Medicine, University of Colombo, Rajagiriya, Sri Lanka Consultant physician in Panchakarma and Kayachikithsa, Ayurveda Teaching Hospital, Borella, Sri Lanka Sri Lankan traditional medicine is probably over 5000 years old and rich with various medicinal preparations and treatment methods including Sri Lankan Traditional Panchakarma. When performing Vamana Karma, traditional physicians use many ingredients mentioned in Ayurveda texts. Knowledge of some herbs used in Vamana Karma, such as Wilandavenna (Lindernia pusilla), Bim Nuga (Tylophoraindica and Tylophora flava), Kotadimbula (Ficus hispida), Bakmi (Nauclea orientalis), Nitul (Streblusasper) and Tholabo (Crinum asiaticum) is confined to families of these Sri Lankan traditional physicians. Methods of performing Vamana Karma by Sri Lankan traditional physicians differ somewhat from classical Ayurveda methods.Dr. H.M. Karunarathne, a traditional physician from Deegalla Bogoda Veda Parampara performs Vamana Karma using Linderniapusilla (Wilandavenna).Thirty g rams of fresh L. pusilla (Wilandavenna) are pounded. This is mixed with 750 ml of fresh cow's milk to prepare the Vamana Aushadha (Emetic drug). Then the mixture is strained seven times. While straining, the physician takes seven steps forward and three steps backward. The traditional physician believes that the number of bouts of vomiting will be equal to the number of steps taken forward. The number of backward steps will be the number of times which purgation will occur.The patient is given a 240ml of decoction of Kalashaka (Murray koenigi) mixed with 5ml of cow's ghee in the mornings for three days before Vamana Karma. On the 4th morning, before Vamana Karma, the patient carries out religious observances and the physician will chant traditional Mantras. Then, both physician and the patient sit with their backs in the direction where “Maru” (evil forces of death) are residing on that day. While maintaining their position Vamana Aushadha is administered to the patient orally. When the patient feels the urge to vomit, king-coconut water is given to facilitate vomiting until the appearance of Pitta (bile) in the vomitus. During the process the patient may get loose motions a few times. When Vamana Karma is completed, the patient is given a special diet consisting of rice and green gram curry as lunch. The patient is advised to follow a proper dietary and behavioral regimen. It can be stated that Linderniapusilla (Wilandavenna) is a safe emetic drug inherent toSri Lankan Traditional medicine. Keywords: Wilandawenna, Panchakarma, Vamana Karma, Emetic 4 Group A. Combination therapy of Danta Śūla. Trincomalee Campus. Mukhadurgandhyatā. Kapha and Rakta Doṣas. Kadukai Lehiyam is a siddha formulation which is mentioned in Gnapadam Porudpanpu Nool part – 1. respectively. a chronic disease. B and C were given Patolādi Lepa Pratisārana. Hence here. gas expulsion was also noticeably reduced. A 65 years old male patient reported decreased stool frequency. Malakaddu (Constipation).OP 01-01: effectiveness of Patoladī Lepa Pratisārana and Mustādī Taila Gandūṣa in the management of Kṛmidanta – A comparative study LSP Kumaradharmasen1. Improvement in Chidrata and Krishnata was not observed. Mustādī Taila. He has had past history of above illness. Śopha.A case study N Krishnamoorthy Unit of Siddha Medicine. This study indicate that combined therapy can be used for early stage of Kṛmidanta as prophylactic measures. Appreciable results were obtained after 3 days onwards in the form of increasing in frequency of defecation. Sri Lanka 2 Gampaha Wickramarachchi Ayurveda Institute. KPP Peiris2. The sample was 90. Further. Moolam (Hemorrhoids). Danta Harṣa.Vayu (Flatulence). straining during defecation and feeling of incomplete evacuation for 3 years.001 in Group A and B. Satisfaction of patient and Urge for defecation. The texture of stool is rough and hard. Keywords: Patolādī Lepa. Soolai (Abdominal pain). All three groups were shown to have significant values of salivary pH and OHI. 2% to 20% of the population suffers from Constipation and most of them are adults. University of Kelaniya. which is manifested by vitiated Vāta. This study showed a reduction of Danta Śūla. It is associated with excess gassiness. Kṛmidanta OP 01-02: Evaluation of the efficacy of Kadukai Lehiyam in the management of constipation (Malakaddu) . Animitta Rujā at p < 0. The effect of treatment was assessed by subjective and objective parameters. He has taken fiber laxativeand bisacodyl alternatingly for relief. Patoladī Lepa and Mustādī Taila are prescribed in Chakra Datta as local remedies for “Kṛmidanta”. SV Kamal3 1 Gampaha Wickramarachchi Ayurveda Teaching Hospital. Kṛmidanta and Dental Caries have the same clinical features. Eastern University of Sri Lanka In the world. an effort was made to evaluate the efficacy of Kadukai Lehiyam in management of Constipation (Malakaddu) through a case study. More than 80% of Sri Lankan and 36% of world population are suffering from Dental caries. Danta Harṣa. Sri Lanka Dental caries. B and C who were selected by using simple random sampling method. Chalatā. University of Colombo. Mukhadurgandhyatā. It can be used to cure Agni Mantham (Gastritis). is unique among humans and is one of the most common global oral health problems in the world today. Literature review of ingredients of the drug shows that most of them have laxative and carminative potency. The patient was treated with 10g (Punnai fruit size) of Kadukai Lehiyam at night after meals with Luke warm water for 40 days. 5 . Śopha. thirty in each Group A. Animitta Rujā also revealed statistically significant results. Sri Lanka 3 Institute of Indigenous Medicine. Mustādī Taila Gandūṣa and combination of above drugs. University of Colombo. to add to the existing knowledge of pharmaceutical significance of the above formula. In Sarasankshepaya. Sri Lanka 3 Department of Pathology. Faculty of Medicine. indigenous medical text written in Sanskrit. University of Colombo. this work was chosen. laxative OP 01-03: Literary Study on Indigenous Version of Nārāyana Taila WGNS Samanweera1. Sinhala Yogarathnakara has NarayanaTailaya in Sinahala verse and Sneha Shatakaya includes a formula in Sanskrit verses. Sinhala Yogarathnakara. DL Perera2 1 Department of Dravyaguna. The aim of this study is to compare the indigenous formula of Narayana Taila found in a palm-leaf manuscript and to highlight the unique characteristics of the indigenous version. Madhyama Narayana and Maha narayana. therapeutic applications OP 01-04: Evaluation of polyherbal formula PhalaKalyāṇa Ghṛta on sperm parameters of Sprague Dawely rats KC Perera1. Sri Lanka 4 Department of Biochemistry and Molecular Biology. medicinal oil. It is recommended to keep the juice concoction in a vessel sealed with clay for seven days and use cinnamon firewood for heating for three days to be processed with sesame. The formula of Maha Narayana oil is mentioned in Ayurveda Pharmacaopea of Sri Lanka and the name Maha Narayana Tailam is found in Indian Ayurvedic sources like SahashraYogam too. maduka. Sri Lanka PhalaKalyāṇa Ghṛta (PKG) is an herbal medicine used by Ayurvedic medical practitioners in Sri Lanka to treat infertility. D Mathew4 1 Institute of Indigenous Medicine. So. margosa. Sneha Shatakaya. Faculty of Medicine. D Lokuhetty3. and palm-leaf manuscripts were used as primary data sources and data were verified with views of indigenous medical practitioners. Gampaha Wickramarachchi Ayurveda Institute. there are three types of Narayana Taila formulas found namely Laghu Narayana. Sri Lanka 2 Department of Obstetrics and Gynaecology. University of Colombo. Eighteen rats were randomly divided into three groups (n = 6 /group). Keywords: Kadukai Lehiyam. Indigenous formula of Narayana Taila includes 44 plant ingredients of which the fresh juice is used for as the main medicinal concoction while all the other formulas contain the decoction of the dried raw materials.This case study revealed that Kadukai Lehiyam is very effective in the management of constipation (Malakaddu). For this study Sarasankshepaya. S Wijeratne2. Faculty of Medicine. Sri Lanka Naryana Tailaya is a famous medicinal oil preparation used among indigenous medical practitioners and popular among general laymen society too. Sri Lanka 2 PostgraduateInstitue of Archaeology. Rats in group 1 and group 2 were orally administered 900 and 1800 mg /kg body weight of 6 . University of Kelaniya. castor oils and ghee. Keywords: Nārāyana taila. constipation. The therapeutic applications of this oil are very diverse and the pharmaceutical studies to be carried out on the manufacturing process which is unique to the Sri Lankan indigenous medical tradition. University of Colombo. University of Kelaniya. indigenous formula. Therefore. MMM Rifaee1 1 Institute of Indigenous Medicine. than control group. MSM Nasmeer1. (0. Unani medicine 7 . Group 3 were considered as control and given distilled water. arthritis. University of Colombo. Keywords:PhalaKalyāṇa Ghṛta.68±0.headache.Terminalia chebula.73 ± 2.001) and Group 3. was significantly higher (48. (32.1±4.21 x 106 ml) compared to Group 1. the healthy motile sperm count.67 ± 6. Improper function of the immune system leads to various ailments such as allergy.antifungal. (3.001). morphology and testosterone level. immunomodulators. antibacterial. asthma.anti-inflammatory.Emblica officinaliswhich are believed to boost or modulate the immune system and thus prevent or treat disease conditions. rats were weighed and blood samples collected for hormonal assay.Theinformation collected from classical texts.001) rats. Ithrifal e Kishneezis a Unani compound preparation which is commonly used in treating diseases like chronic catarrh.the PKG. Sperms were collected from cauda epididymis into warm Ferticult medium for sperm analysis. Then they were sacrificed and male reproductive organs dissected out. Many people resort to herbal medicines in seeking immunomodulators in order to keep them healthy. motile sperms OP 01-05: Immunomodulatory effect of Unani Compound preparation Ithrifal e Kishneez JF Nairoza 1.13 X106 mL) (p = 0. respectively when compared to the group 3. Motile sperm concentration was significantly higher in group 2. antipyretic. Scientific Journals. fertility. sperm motility and sperm morphology were assessed as per WHO criteria. gastric problemsand it acts as a stimulant. Total motile sperm concentration. Serum testosterone level was significantly elevated in both treated group 1 and group 2 (2. respectively for 12 weeks. Immunomodulators are substances that modulatethe immune system through a positive or negative manner to help the body resist foreign pathogens and infectious agents.21 x 106 ml) (p = 0. Sprague Dawley rats. (18.11ng/mL) (p = 0. Sri Lanka 2 Rural Ayurvedic Hospital. Sri Lanka The immune system is a complex defense mechanism of the body which protects the body against infectious organisms and preserves the health of human beings.00 ± 5. Thirukkovil. Keywords: immune system. MFM Fahim 2.Ithrifal e Kishneez can be effectively used as an immunomodulatory drug to strengthen the immune system and protect the body from diseases. The ingredients of Ithrifal e Kishneez showed immunomodulatory effects as well as antioxidant. books. The mean Sperm count of Group 2 rats.It contains important ingredientssuch as Coriandrumsativum. The objective of the study was to find out immunomodulatoryeffect of the ingredients of Ithrifal e Kishneezin order to justify its long use in Unani System of Medicine.PKG possesses positive effects on sperm parameters of male Sprague Dawley rats which was evident by increasing sperm concentration. anti- carcinogenic activities and hepato-protective activity. antiviral.10 ng/mL). cancer and other infectious diseases.967± 0. After the treatment period.Terminalia belerica.Review articles and websources (PubMed and Google Scholar databases) were thoroughly studied. Ithrifal e Kishneez.67ng/mL). interviews with traditional practitioners and 12 classical Ayurvedic text books.S.OP 01-06: Diversity and utility of treatments recommended for Pramēha and associated conditions in Sri Lankan indigenous system of medicine WGNS Samanweera1. Gampaha Wickramarachchi Ayurveda Institute. The aim of this study was to find the unique features of diversity and utility of treatments recommended in indigenous medicine in contrast to classical Ayurveda. according to both Ayurveda and indigenous medicine. Pittu. University of Kelaniya. Sri Lanka Urolithiasis (Urinary Calculi. Primary data were collected from 34 indigenous medical texts.A. But the diversity of plants and preparations mentioned in IM is high and some of them are exclusively found only in the Sri Lankan tradition. indigenous medicine. Páṇi. Piper longum and Strychnos potatorum was given to the patients of group A (n=16) at a dose of 10g with 240ml of young coconut water three 8 . in the management of urinary calculi. Talapa. It is suggested to conduct further research on pharmacological properties and biologically functional compounds of exclusive ingredients and preparations found only in indigenous medicine. University of Kelaniya. Five well practicing traditional practitioners were selected from five provinces and data were collected using a pre tested questioner. According to IM texts and practitioners. preparations.Kodikara. Muthra Ashmari in Ayurveda) is a common disorder with an increasing incidence and is reported to occur in 10-15% of the global population. Káwum are exclusive preparations mentioned in IM. The collected data were compared to find the differences between traditions of classical Ayurveda and indigenous medicine (IM). Faculty of Medicine and Allied Sciences. Rajarata University of Sri Lanka Pramēha is one of the chronic conditions that needs vigilant care and prolonged therapeutic management supported with a restricted dietary routine. 29 patients with urinary calculi confirmed by a consultant radiologist were selected from Out Patient Department of Gampaha Wickramarachchi Ayurveda Teaching Hospital and assigned into two groups randomly. Kanda. There is no significant difference found in basic principles and theoretical approach applied for treatment context in Ayurveda and indigenous medicine. 235 medicinal plants were identified in treatments for Prameha. Lēha. a descendent from king Keerthi sri rajasinghe(1747-1782). A large variety of medicinal preparations such as Basna.K. WAL Chandrasiri1 1 Gampaha Wickramarachchi Ayurveda Institute/University of Kelaniya. Kāyam. This randomized prospective cohort clinical study was designed toevaluate the effect and efficacy of herbal formulation selected from Ola leaves manuscript belonged to Dr. S Pilapitiya2 1 Department of Dravyaguna.P. The powder of plant materials consisting of Elettaria cardamomum. treatments OP 01-07:Evaluation of the effect and efficacy of a herbal formulation derived from Sri Lankan medical Ola leaf manuscript in the management of urinary calculi SMSU Gnanarathne1. Sri Lanka 2 Gampaha Wickramarachchi Ayurveda Institute. Keywords: Pramēha. The word Diyavediyāva is found in IM texts in later periods as a common synonym for Pramēha but exclusively used for diabetes. Pattiyam Allīma is an important part of Pramēha in IM and a wide treatment system is hidden in Veda Paramparā as well as in lesser known literary sources. plants. Lēpa. Sri Lanka and Department of Medicine. These results provide scientific back ground for further studies on the effect and efficacy of this herbal formulation in order to validate the success in the management of urinary calculi. burning micturition. haematuria. pyuria.05) and reduction in the number of stones(83. pain.times a day for consecutive 21 days. The patients of group B (n=13) were treated with 2 tablets of Cystone (Himalaya) 3 times a day for 3 weeks comparatively.66%) without significant difference between two groups. and tenderness at renal angle(P<0. The patients of Group A showed significant effect on reducing clinical features i. Keywords: calculi.e. the patients of Group B showed significant effect on reducing pain.33%) and size of stones (66.05) and decrease in the number of stones(73. Similarly. herbal formulation 9 .5%). pyuria(P<0.33%) and size of stones(62. 10 . Track 02 Integrative. evidence based traditional medicine practices Abstracts of Keynote Speakers and Oral Presenters 11 . the Ministry of Public Health launched the health information reform program to reduce the burden of data collection and recording in the operation system and to acquire essential data in a simple and non-redundant manner. The data and information acquired include those on diagnoses and treatments. causing no burden to local health officials. monitoring and evaluation 12 . the system is developing a system for reporting on traditional Chinese medical services and procedures and home visits in the communities. Such a system is used as a tool for monitoring and reporting the performance of the TTM service provided for outpatients and inpatients at all health-care facilities across the country. . Thailand The Department of Thai Traditional and Alternative Medicine in Thailand was established in 2002 to play an important role in developing the Thai traditional and alternative medical (TTM) service system so that it is implemented along with the modern medical system. Technical and Plannning Division. Real-time Monitoring System of Thai Traditional and Alternative Medical (TTM) Service Performance in the Health Service System of Thailand Dr. health promotion activities and proportions of services. In 2014. Rutchanee Chantraket Head of Technical and Knowledge Bank Working Group. This effort is carried out in cooperation with the Information Technology Center in communicating to develop a Health Data Center for Thai Traditional Medicine (HDC TTM) Services. Department of Thai Traditional and Alternative Medicine. therapeutic procedures. The national database system has been developed to collect and record service data in 43 standard folders that correspond to the need for data utilization at the local level and such data can be forwarded to the central administration. drug dispensing. At present. Ministry of Public Health. Keywords: management information system. 43 standard folders of data. system of traditional medicine products. Thailand The pharmacovigilance (PV) system of traditional medicine products. more than 2.During 1999-2016. The scope of surveillance covered both registered and unregistered products. The system was established in 1983 under the supervision of Thailand’s national drug regulatory authority (Thai Food and Drug Administration: Thai FDA). especially after traditional medicine was included in the Thai National Essential Drug List (NEDL) in 1999. out of those reports two signals for the product containing single herbal medicines were detected. Cassia siamea (leaf).The Pharmacovigilance System of Traditional Medicine Products in the Health Service System of Thailand Ms. such asa two-year Intensive Monitoring Programme (IMP) for single herbal ingredient formulation. associated with hepatotoxicity in 2000 and serious hypersensitivity reactions from the use of Andrographis paniculata in 2012. Herbal products 13 . was set up as a part of the national vigilance system.000 reports of traditional medicine productswere received. To promote the reporting of adverse events of traditional medicine.including herbal products. Former Head of the Health Product Vigilance Centre of the Thai FDA. Keywords: Pharmacovigilance. Back then. in line with the requirements of the NEDL 1999 and 2006 editions. The registration of products containing Cassia siameawere withdrawn and warning of hypersensitivity was added for Andrographis paniculata. there was no mechanism set up for quality control in laboratories to investigate the product in routine practices unless a cluster of cases were detected or adulteration was suspected. the risk of taking these products were disseminated to health-care professionals across the country following appropriate regulatory action. Aftersignals were generated. several active surveillance programmes were initiated. Wimon Suwankesawong Director of Technical and Planning Division. National Pharmacovigilance Center.The first report of adverse eventsof traditional medicine was received in 1999. Lactoferrin. elevated levels of reactive oxygen species (ROS) was observed as a result of GG-ZnFe-Fe-bLf treatment. Magnetic nanoparticle therapy has gained immense attention over the years. investigation of the apoptotic pathway taken up by the GG-ZnFe-Fe-bLf nanoparticles was determined via western blots. The core exhibited crystalline structure and the lactoferrin was successfully encapsulated which was confirmed using XRD and FTIR respectively.4Fe2. which depicted an upregulation of p53 and FAS and down regulation of survivin and Bcl-2.6O. Apoptosis 14 . It has the potential to provide targeted anti-cancer therapy. Pivotal issues concerning the use of chemotherapy as a treatment alternative are its off-target effects. Nanoparticle characterization carried using TEM indicated a spherical morphology and size range of 20-50nm. Keywords: Guar gum. Nanoparticle internalisation occurred in 2 hours via the Lactoferrin receptors present on colon cancer cells surface. Anti-Cancer Activities of Guar Coated Zinc-Ferrite Magnetic Nanoparticles Encapsulating Lactoferrin Prof. a novel approach was performed using adsorption. Colorectal cancer is the third most common cancer in men and women. Australia. 3D caco-2 tumor model confirmed the ability of GG-ZnFe-Fe-bLf in reducing the surface area and viability of cells. real time imaging and thus. GG-ZnFe-Fe-bLf did not significantly alter the viability of FHs 74 cells.Fe2O3) nanoparticles were coated with Fe-bLf encapsulating guar gum. adverse side effects and drug resistance. Jagat Rakesh Kanvar Leader in “Nanomedicine-Laboratory of Immunology and Molecular Biomedical Research” at Deakin University. Zinc ferrite.this was confirmed by trypan blue and MTT assay which was observed in caco-2 and SW480 cells. Colon cancer. Additionally. Further. Therefore. wherein magnetic zinc ferrite (Zn0. However. Adsorption. Additionally. theranostic properties with a significant reduction in side effects. Enhanced anti-cancer efficacy of nanoparticles was observed when used in the presence of a magnetic field. Presence of protein shell improves solubility and stability in physiological solvents and allows selective targeted delivery of the cargo. Protein capsule. Imaging of tissues is of great importance for improved diagnostics. Keywords: bioimaging. is used to encapsulate imaging agents and therapeutic molecules. Application of nanomaterials in medicine has a great potential to refine the bioimaging capabilities enabling tracking the delivery of a therapeutic compound. Applications of Nanotechnology in Bioimaging and Drug Delivery Dr. Novel nanoparticles were developed and allow combined deep tissue fluorescence imaging in the near-infrared wavelength range of low absorption of biological tissues and magnetic resonance imaging. Lyudmila Turyanska Assistant Professor/Senior Lecturer in the School of Physics and Astronomy (University of Nottingham) and School of Chemistry (University of Lincoln). drug delivery 15 . Apoferritin. USA. The talk will review recent development of nanomaterials for applications in bioimaging and drug delivery. thus enhancing treatment selectivity. normalization of HPA-axis and autonomic reactivity. they remain difficult to treatdue to issues of treatment resistance. As such. among others. Canada Clinician Scientist. Complementary and Alternative Medicines (CAMs) are interventions not considered part of conventional Western medicine. nutraceuticals. and non-compliance. such as exercise and Yoga. CAMs have been shown in several trials to have clinicalbenefits in mood and anxiety disorders.Treating Depression and Anxiety: Integrating Complementary and Alternative Therapies in Clinical Practice Prof. Depressive and anxiety disorders are leading causes of disability worldwide and major contributors to the global burden of disease. It will also provide suggested guidelines for integrating CAMs with conventional Western interventions. and reduction of inflammation. such as St John’s wort and curcumin. Centre for Addiction and Mental Health. nutraceutical 16 . Department of Psychiatry and Director. These interventions – many of which have been used for generations in the East – have gained popularity in the West in the last couple of decades. University of Toronto. reflecting a paradigm shift in Western medicinetowardsholistic approaches that integrate elements of Eastern medicine. there is reasonable evidence that they are similarto those of conventional Western medicines. Arun V. Canada. Toronto. Global Mental Health and the Fellowship programs. adverse effects. there is increasing interest in alternative therapeutic approaches. Despite considerable research. Keywords: depressive and anxiety disorders. among others.These include modulation of neuronal signaling and connectivity. The presentation will review the published evidence for the benefit of different CAM treatments for depression and anxiety together with proposed neurobiological mechanisms. Ravindran Professor. complementary and alternative Medicine. While thepathophysiological mechanisms mediating their benefit are not fully understood. These include physical therapies. Yoga. andherbal remedies. such as omega-3 fatty acids. 6) Monitoring and assessing. which includes a) Database for clinical evidence. 4) Ethical and evidence-based policy. wellness. To address the global challenges in the area of traditional. is conducting the following activities in accordance to WHO roles in public health: 1) Leadership. proper use and effectiveness of T&CM by regulating T&CM products. Integrative Medicine.18) which is focusing on the implementation of the strategy. complementary and integrative medicine(TCI). World Health Organization. research and integration of T&CM products. 2)To strengthen quality assurance. b) Networking. The implementation of the strategy will be conducted together with the WHO Strategy on People-Centred and Integrated Health Services. The World Health Assembly in 2014 also adopted a resolution on traditional medicine(WHA67. Complementary and Integrative Medicine Unit. d) 'Benchmarks for Practice' in TCI. The strategy covers two goals: 1) Harnessing the potential contribution of traditional and complementary medicine(T&CM) to health. 5) Building institutional capacity. Keywords: Traditional. which includes a) Development of technical documents. practices and practitioners into the health system. b) TCI Knowledge platform. b) International Terminology and Classification of TCI. which includes a) Country support in the implementation of the WHO Traditional Medicine Strategy. safety. as appropriate. practices and practitioners. in coordination and cooperation with Regional and Country Offices. WHO developed and launched the WHO Traditional Medicine Strategy 2014- 2023.Implementation of WHO Traditional Medicine Strategy 2014- 2023 Dr. 3)To promote universal health coverage by integrating T&CM services into health care service delivery and self-health care. 2)Promoting safe and effective use of T&CM through the regulation. norms and technical documents. 3) Standards. 2) Research and Knowledge. The three objectives of the strategy are: 1)To build the knowledge base for active management of T&CM through appropriate national policies. and contribute to the goal of universal health coverage. which includes a) Integration of TCI in national health systems. Traditional Medicine Strategy 17 . people-centred health care and universal health coverage. Geneva. Traditional. Zhang Qi Head. The WHO headquarters. c) 'Benchmarks for Training' in TCI. which includes a) Interregional training workshops for capacity building of government officials b) Development of capacity building tools. Rajagiriya.reform and empower the Institutes working on ITM research. empower Ethics Review Committees reviewing research on ITM. J. GLP. The aim of this study was to review the Sri Lankan situation of research governance and find suitable strategies to advance the research on ITM. S.. The result revealed that no national research governance policy currently exists inSri Lanka. Open educational opportunities to follow research methodology. responsible conduct of research. essentials of research administration. The related material was collected by literature survey and firsthand experience. M. offer equal opportunities for research on ITM in draft National Health Research Act. GCP. An Overview of Strategies for Improving Research on Indigenous and Traditional Medicine Dr. Institute of Indigenous Medicine. The Sri Lankan Indigenous and Traditional Medical System (ITM)are as old as the civilization of the country. human subjects’ research. prepare guidebooks on research ethics specially forITM. conflict of interest. validate subjective criteria. develop a monitoring body for research on ITM and remove the obstacles which hindered the research on ITM. animal care and use. Good Clinical Practice (GCP) and Good Laboratory practice (GLP) etc. Sri Lanka 18 . formulate healthcare ethics committees. Keywords:Indigenous and Traditional Medical System. international content. W. information privacy and security. Research in Indigenous and Traditional Medical systems are a new growing trend of the globe. increase collaborative research. It is a necessity to provide equal states for ITM in National Health Policy. University of Colombo. Comprehensive strategies are also inevitable to evaluate traditional and indigenous medical prescriptions for the advancement of the field. identify Key terminology. prioritize research fields. bio safety and biosecurity are need to empower the ITM community in Sri Lanka. Research. including ethical guidelines or codes of conduct for research. follow ethical conduct. Sri Lanka. Kumari Department of Basic Principles. In fact. treatment methods. which are said to be still effective and accepted by the community. 19 . Some ancient cities of Sri Lanka. Polonnaruwa. there are many valuable medicine. and Gedi-Wana-Pilika Wedakama (treatment of abscesses. University of Colombo. Medirigiriya. The Moneragala District in the Uva Province consisting of eleven divisional secretariats is selected for this study as its territory is rich of different indigenous medical traditions and also endemic medicinal plants. Diversity of Indigenous Medicine of Sri Lanka with Special Reference to the Moneragala District Dr. Institute of Indigenous Medicine. S. beliefs and techniques in some families coming from generations which are still undocumented. Sri Lanka. Helawedakama. Unmada-Wedakama (psychiatry). Keywords: Indigenous Medicine. Historically. Sri Lanka is proud to claim to be the first country in the world to have established systematic hospitals. Department of Deshiya Chikitsa. Anuradhapura and Mihinthale still have the ruins of what many believe to be the first hospitals in the world. Samarakoon Senior Lecturer.S. indigenous physicians enjoyed a noble position in the country's social hierarchy due to the royal patronage granted to them by ancient kings. Indigenous Medicine (IM) "Helawedakama" is a heritage of Sri Lanka coming over centuries from generation to another. In addition. wounds and cancers) etc.M. Moneragala District. Es-Wedakama (ophthalmology). Sarpavisha-Wedakama (treatment for snake bites). Indigenous Medicine of Sri Lanka comprises numerous branches such as Kedum-bidumWedakama (orthopedics or fracture healing). GS demonstrated significant reduction in VAS scores. needle aspiration and surgery. Keywords: osteoarthritis. Primary efficacy measures were decreased severity of affected knee pain. pain and after confirming safety in clinical trial on patients with osteoarthritis (OA). Bangalore. Sri Lanka Granthi can be small glandular or nodular swellings due to benign tumours (neoplasms and cysts).01) in use of rescue medications. Clinician Global Impression Change OP 02-02: Effect of Agnikarma in the management of Asthijagranthi with special reference to ganglion on the wrist joint . A ganglion cyst is a fluid filled lump associated with a joint or tendon sheath. According to Vagbhata.A case study KCP Gunarathne Gampaha Wickramarachchi Ayurveda Hospital. which is attributed to the active constituents Curcuminoids.OP 02-01: The therapeutic potential of Curcuma longa Linn [Turmeric]: A clinical trial using Turmacin for osteoarthritis C Kulkarni1. WOMAC and CGIC scores on day 21 and 42 compared to placebo. India 3 St John's Medical College & Hospital. Visual Analogue Scale. a well-known Indian spice from rhizomes of Curcuma longa is used in Indian cuisine and Ayurvedic medicine as an anti-inflammatory. St John's Medical College. Turmacin [NR-INF-02] was studied in animal models of inflammation. Allopathic treatments include watching and waiting. benign tumors that have not suppurated are treated in a manner similar to swelling. Bangalore. The study demonstrated safety and efficacy of Turmacin. India 2 St John's Medical College. India Turmeric. Asthija Granthi is a protuberant or depressed Granthi developed after Abhighata (fracture or trauma). Bangalore. Most are asymptomatic but some can develop complications. Safety and efficacy of Turmacin was assessed in knee OA by randomized placebo-controlled. Traditional medicine shows water soluble components are consumed as curries and decoctions for health benefits. Bangalore and Division of Clinical Pharmacology. which has prompted hypotheses that water soluble phyto- constituents possess pharmacological activity.05) decrease in VAS. K Madhu2. They most often occur at the posterior aspect of the wrist. clinical trial. MJ Saji3 1 SAKRA World Hospital. Turmacin. 120 primary OA patients received placebo(400mg twice daily) or Turmacin(500mg twice daily) or glucosamine sulphate (GS) (750mg twice daily) alone or in combination for 42 days. splitting the affected joint. Efficacy was assessed on day 21 and 42. McMaster Universities Osteoarthritis Index. The turmacin treated group showed a significant(p≤0. adding supportive evidence to the protective activity on cartilage and is currently being evaluated for application in sports and long term benefits in OA using Magnetic Resonance Imaging (MRI). Extracts from an aqueous base devoid of Curcuminoids were studied in vitro and in vivo. with decrease(p≤0. and function by Western Ontario and McMaster Universities Osteoarthritis Index(WOMAC) scale. Clinical examination was by using Clinician Global Impression Change(CGIC). A girl aged 25 years presented to the hospital out-patient department 20 . using a Visual Analogue Scale (VAS). single- blind. University of Kelaniya. Hela. Sri Lanka Cancer is a disease involving abnormal cell growth with the potential to invade or spread to other parts of the body. University of Kelaniya. Keywords: ayurveda. MTT and Alamar blue assays were carried out to evaluate the in-vitro cytotoxic and proliferative effect on Endometrial Carcinoma (RL95-2). MTT assay 21 . Internal and external Shothahara (reduce swelling) and Lekhana (emaciation) treatment was started and progress monitored for three months. It can be concluded that there is no cytotoxic effect of tested concentrations of MMYACH choorna and it can be used as an anti-proliferative drug on Endometrial carcinoma. the tested concentrations of the decoction had no significant proliferative or anti-proliferative effect on Hela.5cm . Gampaha Wickramarachchi Ayurveda Institute. the effect of MMYACH choorna. This case study suggests that Agni karma is an effective method among Parashalya (para surgical) methods. Hela. there is still no effective treatment found for most cancers. Agni Karma. or Asthijagranthi. JA Liyanage3. In this experiment. Ganglion. One of the major causes of death worldwide. Faculty of Veterinary Medicine and Animal Science. It was mobile and had very slow growth. on in vitro cytotoxicity and proliferation is evaluated. MTT assay was carried out to determine the cytotoxicity with non-treated cells as negative and ethanol as positive controls respectively. in Ayurvedic terms. University of Peradeniya. Faculty of Science. Sri Lanka 3 Department of Chemistry. After 24 hours. Fine Needle Aspiration diagnosed a ganglion. For MTT assay.1cm in diameter and it started softening from a hard consistency. cells were seeded in 96 well plates and after 24 hours incubation. however no change was seen. But for RL95-2 cell line the results showed a significant anti-proliferative effect in each of the tested concentrations of the decoction. Apunarbhavakara OP 02-03: Evaluation of the in-vitro cytotoxic and proliferative effect of MMYACH choorna on Endometrial carcinoma. Keywords: Granthi. treated with different concentrations of decoction.05. Parashalya. cytotoxicity. Sri Lanka 2 Department of Kaumarabruthya and Shree Roga. HP Wakkumbura2. details of which cannot be published yet due to application of patent rights. Vero and L929 cell lines. MTT assay results showed that the concentrations tested were not cytotoxic to any cell line compared to controls with p<0. Vero and L929 cell lines WMD Wickramaarachchi1. a novel herbal decoction of 9 Sri Lankan medicinal plants. Finally Agni Karma (cauterization) was done weekly by Panchalauhashalaka (an instrument). TN Premachandra1. Alamar blue assay was carried out similarly in 24 well plates.with a symptomless swelling on the left wrist for the last five years. RPVJ Rajapakse1 1 Department of Veterinary Pathobiology. In Alamar blue assay results. Within one month the swelling started to reduce in size from 1. endometrial carcinoma. Vero and L929 cell lines. Pāndu. The antidotes used for metallic preparations can have metal binding properties that can have an effect on the chelation process.OP 02-04: A comparative literature review on different methods of iron chelation therapy in allopathic medicine and Ayurveda WDD Weerapperuma1 JMS Udayakumara2 1 Meegoda Ayurveda Provincial Hospital. TripalādiAveleha contains many ingredients that have shown the chelating activity. Ministry of Health. iron chelation therapy. Keywords: Thalassemia. flavonols and anthocyanin contain an iron-binding motif similar to the catechol moiety that is a known iron-binding element of microbial siderophores. Sri Lanka 2 Teaching hospital Mahamodara. Plīhavriddhi etc. Proanthocyanidins. Sri Lanka Thalassemia is one of the inherited blood disorders caused by a single gene error that leads to abnormalities of haemoglobin production. Atihraswa. epicatechins. The main complication of thalassemia is iron overload and modern medicine prescribes regular iron chelation therapy with dexferioxamine for patients unable to afford bone marrow transplantation. The objective of this study was to review literary sources to find the logical basis of iron chelation therapy according to Ayurvedic principles. experimentation of alternative methods for chelation is a timely need. Since contemporary surveys show that patient compliance to dexferioxamine is low. The conclusion suggests carrying out experimental studies to prove the iron chelation activity of Ayurvedic preparations that can increase thalassemia patients’ compliance and transfusion intervals. Experimental models suggest that foods containing plant polyphenols and flavonoid compounds may have benefits as iron chelators that can even cross the blood–brain barrier. Analyzing the treatment protocols of Paandu and Kaamala shows that natural chelating agents are commonly found among the ingredients of prescribed recipes. Kāmalā. Kāmalā 22 . The methodology used was to analyze the treatment protocols and research data on iron chelating agents as an alternative or adjunct treatment of thalassemia. Ayurvedic drugs like DhātrīAvaleha. The clinical manifestations can be described in terms of Ayurveda like Pāndutā. Our results indicate a trend that consumption of tea (>3cups/day) and dairy products (>2 times/ week) may have a positive effect on reduction of the progression of cognitive decline in HD and SCA. Faculty Health. Rajarata University of Sri Lanka 3 Faculty of Medicine and Allied Sciences. The 23 . spinocerebellar ataxia. KDPM Weerasinghe1. anti-oxidant activity is important to control the complications of Diabetes (Prameha). Sociodemographic. Colombo. 3 cups/day vs <2cups/day (p=0. cognitive decline OP 02-06: In-vitro assessment of anti-oxidant activity of Mathumeha Chooranam (MC) used in Prameha RP Mendis1. Maastricht University. thus. 2 time/month vs 1 time/week (p=0. TK Nawaratne1. dairy products consumption vs scores of ACE-R. number of tea cups vs total score of ACE-R. University of Sri Jayewardenepura. tea. H Gunasekara3.03) and number of tea cups vs orientation/attention sub scores of ACE-R 3cups/day vs >3cups/day P= 0.Revised (ACE-R) respectively in a Sri Lankan hospital based population.59yrs) were recorded using standard questionnaire and Sinhala translation of Addenbrook’s Cognitive Examination .03) were found. P Akalanka1. Faculty of Medical Sciences. Kurunegala. Cognitive decline is characterized in Huntington Disease (HD) and Spinocerebellar Ataxia (SCA). LPTB Levangamage3.7. Netherlands 7 EURON .01 were found. The selected medical formula ‘Mathumeha Chooranam’ (MC) is a well-known Siddha drug used in the treatment of Prameha. Faculty of Medicine and Allied Sciences. India 6 Dept. A Dalal5. D Sirisena2. Medicine & Life Sciences. HWG Samanmali1 1 Bandaranaike Memorial Ayurveda Research Institute.OP 02-05: Effect of dietary patterns on cognitive decline in spinocerebellar ataxia and Huntington Disease L Gonawala1. N Wijekoon1. In HD patients (mean age of onset 40yrs 11). New Delhi.7. it is reported that lifelong dietary habits may also have important implications. Translational Neuroscience. This may further provide an insight towards systemic evaluation of the effect of dietary patterns as a modifying treatment for cognitive decline in SCA and HD. India 5 Centre for DNA Fingerprinting & Diagnostics. Maastricht. Keywords: Huntington Disease. M Faruq4. Sri Lanka 2 Teaching Hospital. Hyderabad. Sri Lanka 4 Institute of Genomics and Integrative Biology. HWM Steinbusch6.7 1 Interdisciplinary Center for Innovation in Biotechnology & Neuroscience. V Suroliya4. age range 22.04) and meat consumption vs Language sub scores of ACE-R. Sri Lanka 2 Department of Biochemistry. P Kahingalage1. W Kumbukgolla2. In SCA patients (mean age of onset 37yrs 9). The relationship between dietary patterns and the subscale scores of ACE-R were evaluated. Rajarata University of Sri Lanka Free radicals significantly aggravate diabetic induced atherosclerosis. Sri Lanka 3 Sri Jayewardenepura General Hospital. age range 25.European Graduate School of Neuroscience Though age is the main determinant of cognition. R de Silva1. clinical information and data on cognitive impairment in genetically confirmed patients with SCA type 1 (n= 22. dietary patterns. V Suriyakumara1. 1 time/week vs >2 times/ week (p=0.58yrs) and HD (n=21. AMHS Attanayake1. Sri Lanka Surgical treatment of perianal fistula can be complicated with anal incontinence due to damage to sphincters. 20μg/mL and 30μg/mL) for the antioxidant activity and in triplicates.main objective of this study was to evaluate the anti-oxidant activity (IC50) of aqueous and methanol extracts of MC. Fistulae are classified as sub-cutaeneous. 1977. University of Kelaniya.Hgmm. Rajarata University of Sri Lanka 3 Department Anatomy. Faculty of Medicine and Allied Sciences. University of Colombo. Mutilation of a large area could occur during surgery resulting in reduction of resting and squeez anal pressures and further leading to complications such as anal incontinence. SD Pilapitiya2.17 ±1. pericarp of Aralu (Terminaliachebula) and fruit of Nelli (Phyllanthusemblica) in 1:2:2:2 ratio respectively. both the extracts were marginally behind the standard antioxidant. Mathumeha chooranam. The IC50 value of MC extract in water was in-between 10μg/mL .5μg/mL . 60mL of boiled water was added to 15g of drug and the resultant extract was freeze-dried to obtain a light-brown powder. Pre and post treatment anal canal manometry was performed within the study population. it can be concluded that MC shows significant antioxidant activity which would be beneficial for the management of complications of Prameha. The methanol extract showed slightly higher antioxidant activity than the water extract. DPPH Assay OP 02-07: Effects on anal pressure profile in patients treated with Ksharasuthra (a medicated seton) for high anal fistula AAJP Kumara1. The device was somewhat similar to that used by Hancock et al. pre- treatment resting anal pressures were 55. anti-oxidant activity. But the difference was not statistically significant 24 . Faculty of Medicine.19 ±0. Anthony DJ3 1 Department of Shalya Shalakya. The squeeze pressures showed a slight reduction from pretreatment value of 118.. In the 100 patients studied.07±0. however. Sri Lanka 2 Gampaha Wickramarachchi Ayurveda Institute.76. Each sample was analyzed in 4 concentrations (5μg/mL. Gampaha Wickramarachchi Ayurveda Institute. The IC50 of Gallic acid was in- between 2. 1-Diphenyl –2-picrylhydrazyl) assay was conducted to determine antioxidant potential.97 Hg mm and the post-treatment were 55.19 ±1. The DPPH (1. Therefore. In Ayurveda perianal fistulae is treated using Kasharasuthra. supra and extra-sphincteric based on their relationship to the anal sphincter. Gallic acid. 100 patients with supra sphincteric fistulae or with supra levator ani abscess cavity and high trans-sphincteric fistulae were selected for the study.20μg/mL and methanol extract showed the value in-between 5μg/mL . 10μg/mL.57Hg mm to post treatment value of 115. There is no statistically significant reduction in resting anal pressures (p>0. University of Kelaniya. The constituents of MC are dried leaves of Kurignan (Gymnemalactiferum). Pressures in the anal canal were measured with a small water filled latex balloon one centimeter in diameter. trans-sphincteric. a medicated seton. Sri Lanka and Department of Medicine.15μg/mL. Use of Ksharasuthra is believed to cause less disturbance to resting and squeeze anal pressures.5μg/mL. The main objective of this study was to identify the effect of ksharasutra treatment on anal pressures. leaves of Karapincha (Murrayakoenigii). Keywords: Prameha.05). The same extraction procedure was also conducted in triplicate using methanol. High anal fistulas lie above the middle belly of the external sphincter and ano rectal varieties lie above the levator ani muscle.83 Hg mm. Internal and local Shothahara (swelling reduction) and Lekhana (emaciation) medications were started.(p>0. Apunarbhavakara. causing millions of deaths in each year. By fine needle aspiration cytology confirmed as bursitis. medications. On examination a non-tender lump of 4cm in diameter was found with normal skin colour and temperature. Sri Lanka 2 Institute of Indigenous Medicine. The present study was a clinical study on the effect of Bhunimbhadi Ashtadashanga (BA) decoction Vataja Hridroga. Sri Lanka The disease named Granthi denotes a specific character of glandular or nodular swelling. Thirty patients between 30-60 years were selected from the OPD of Gampaha Wickramarachchi Ayurveda Hospital as per the diagnostic criteria after obtaining their written consent and they were treated with BA for four weeks. Within three months it started softening and also reduced in size gradually. Vataja Hridroga which is similar to IHD has been mentioned among the five types of Hridroga. A 65 year old male patient presented to the Hospital OPD with swelling on the posterior aspect of the right elbow joint associated with mild pain occasionally. with one centimeter. University of Colombo. since 10 years. Ksharasuthra OP 02-08: Management of Asthijagranthi with Agni karma W. This study concludes that Ksharasuthra treatment in high anal fistula will reduce complications by preserving anal pressures. Granthi can be small glandular or nodular swellings due to benign tumours (neoplasms and cysts). anal pressures. Agni Karma.05). correlated in Ayurveda with Asthijagranthi. fluid aspiration or surgically removal of entire bursa. can be done.R to olecRanon bursitis – A case study KCP Gunarathne Gampaha Wickramarachchi Ayurveda Hospital. After three months of treatment very mild softness occurred but no reduction in size. prolonged pressure or infection. but hard in consistency. Parashalya OP 02-09: A clinical study on the effect of Bhunimbhadi Ashtadashanga on Vataja Hridroga with special reference to ischemic heart disease EPC Priyadarshani1. Sri Lanka Ischemic heart disease (IHD) is the foremost cause of morbidity and mortality.S. The pain had reduced totally. As allopathic treatments. SKMK Herapathdeniya2 1 Ayurveda Medical Officer. which develops after Abhighata (fracture or trauma) of bones. Keywords: Granthi. A 25 . according to Chakrapani Teeka. Keywords: high anal fistula. In Ayurveda. elbow pads. Asthijagranthi is a protuberant or depressed Granthi. Bursitis occurs due to fluid accumulation in the bursa due to irritation or inflammation by trauma. SMS Samarakoon2. Then Agni Karma was started by Panchlauhashalaka (instrument) weekly. brusitis. Therefore Agni karma (cauterization) can be recommended as the best of para surgical treatment methods which acts as an immune modulator and causes Apunarbhavakara (without recurrence) in most of Chedanartha (excision) cases. Olecranon bursa is flat and situated in the posterior aspect of the elbow joint. According to the data. Data was analyzed by using Wilcoxon signed-rank test and Mann- Whitney U tests.001). Kanchanaraguggulu and Punarnawashtakapaanta were used as oral drugs and Shudhabalataila was used for matravasti.001) and dyspnoea significantly (p<0. IHD. Considering the above effects. When comparing the treatment efficacy between group A (only oral drug treatment) and group B (oral drugs with vasti treatment). Gampaha Wickramarachchi Ayurveda Teaching Hospital. BA reduced pain highly significantly (p<0. whereas the reduction of fatigue and palpitation was insignificant (p>0. Data was analysed by Wilcoxon Sign Rank and paired 't' tests using SPSS software. Matra Vasti 26 .05). The reduction of cholesterol level was highly significant (p<0. HP Wakkumbura Department of Kaumarabrithya and Stri Roga. while it did not show significant efficacy over group A with respect to improvement of endometrial thickness and follicular maturity.complete history was taken before and after treatments. in Polycystic Ovarian Syndrome at 5 percent level of significance. while that of LDL and CHO/LDL ratio was significant (p<0. hirsutism. lipid profile OP 02-10: Effect of Shuddhabala Taila Matra Vasti in cases of Polycystic Ovarian Syndrome SHK Deepthika. Gampaha Wickramarachchi Ayurveda Institute.05). it shows that the treatment of group B was significantly more effective for Polycystic Ovarian Syndrome with respect to oligomenorrhea and Dysmenorrhea. Sri Lanka A clinical study was carried out to find out the effectiveness of Shuddhabala Taila Matra Vasti in the case of Polycystic Ovarian Syndrome (PCOS).05). at Special Sub fertility Clinic. Keywords:Vataja Hridroga. Sixty PCOS patients were randomly selected according to inclusion.05). Lipid profile and ECG were the objective parameters. Keywords: Polycystic Ovarian Syndrome. Symptoms of IHD were taken as evaluation criteria and they were properly graded and evaluated. Data was collected through an interview based questionnaire and findings were seen on day 14 via Trans-Vaginal Scan reports. University of Kelaniya. oligomenorrhea. ECG changes were unchanged and insignificant (p>0. exclusion and diagnostic criteria and randomly divided into two groups as group A and B. Bhunimbhadi Ashtadashanga decoction. Group A were treated with oral drugs and Group B treated with oral drugs and matravasti for one month. it is concluded that BA is effective in relieving the symptoms of Vataja hridroga (IHD). after taking written consent.66%) related to this study. Acne was identified as the most common symptom (65%) and hirsutism and baldness were identified as the least common symptoms (51. Sri Lanka Pakṣāghātais a Vātic disorder and is included in eighty types of Nānāthmajavātaroga. University of Colombo. Sixty patients of Jīrnapakṣāghata of both sexes between the age of 40 to 70 and chronicity more than three years were selected from inpatient department of Gampaha Wickramarachchi Ayurveda Hospital. Sri Lanka 2 Department of deshiyachikitsa.is found to have anti-diabetic properties. Sri Lanka 3 Department of DravyagunaVijnana. WMSSK Kulathunga2. it is shown that the majority of patients were in the age group 40 – 50 years (41. Suddhabala oil Matra Vasti improved speech difficulty and difficulty in moving the distal leg in a highly significant manner (p<0. safe therapeutic modality for the management of diabetes in Sri Lanka. Thirty patients were subjected to Heenmadurutalā leaf powder 2. In this study. When comparing the effect. male (60 %).67%).67%). It is known as Heenmaduruthalā in Sri Lanka. SGPT. Vasti Karma is considered the major Śodhana procedure in Panchakarma. Matra Vasti. Mahakoranda oil Matra Vasti improved speech difficulty. Colombo. PPBS. A Pieris3. Institute of Indigenous Medcine. Gampaha Wickramarachchi Ayurveda Institute. LDAM Arawwawala4 1 National Ayurveda Teaching Hospital. 3 Vasan Care Eye Hospital. The present study is a comparative clinical study to evaluate the efficacy of Suddhabalā oil Matra Vasti and Mahākoranda oil MatraVasti on Jirnapakshaghata. Sri Lanka. Colombo. married (70%) and having secondary education (61. difficulty in moving distal arm and drooping wrist in a highly significant manner (p<0. Sri Lanka 2 Department of Swasthavritta. Mahakoranda oil. SGOT. Nanatmajavartaroga OP 02-12: A comparative study on the effects of Heenmaduruthalā (Ocimum sanctum Linn) and Yoga in the management of Madhumeha (Diabetes Mellitus) HRD Fonseka1. This study concludes that Mahakoranda Oil Matra Vasti is more effective over Suddhabala Oil Matra Vasti in improving Jirnapakshaghata. 4 Industrial Technology Institute. University of Kelaniya. Sixty type 2 diabetes mellitus (T2DM) patients were selected according to inclusion and exclusion criteria. significantly. the improvement of drooping wrist in group B was better than the improvement in group A (p<0. University of Colombo. Serum creatinine and Blood urea were comparatively evaluated to introduce an inexpensive.001). In this multidisciplinary clinical study. Yoga has been known as a body mind medicine for centuries.OP 02-11: A comparative clinical study on the effect of Suddhabala oil MatraVasti and Mahakoranda oil MatraVasti on Jirna Pakshaghata (Chronic Cerebro-Vascular Accidents) HM Chamara1. Sri Lanka. Selected patients were grouped into two groups A and B randomly.001). Yakkala from February 2015 to September 2016. Group A was treated by Suddhabalā Oil Matra Vasti and Group B was treated by Mahakoranda oil Matra Vasti for 21 days. Lipid profile.05). Keywords: JīrnaPakṣāghāta. difficulty in moving proximal arm. the effects of Heenmaduruthalā dried leaf powder and its combination with selected yogic practices on FBS.5g as a single daily dose and others were subjected to five selected yogic practices in 27 . Sri Lanka Ocimum sanctum Linn. Institute of Indigenous Medicine. HbA1c. JTR Jayakody3 1 Gampaha Wickramarachchi Ayurveda Hospital. Data were analyzed by using SPSS software. SMS Samarakoon2. 95 to 116.18±4. Vastikarma is specified for the management of Kshīnashukra in Ayurveda.62±0. the issues have been found to be in the male partner.05) only by combined therapy. T2DM. Drug therapy significantly reduced total Cholesterol from 189. paired t test and independent t test were used in data analysis. PPBS was significantly reduced from 266.3±6.16 to 2.43±7.38±0. Mātrā Vasti. Drug therapy significantly reduced FBS from 179. SPSS.27±31.001) on oligospermia and promoting of sexual health. infertility is increasing alarmingly.addition to the drug for three months. The study was designed as a randomized clinical trial of 60 patients of which 30 were randomly selected for the oral drug as a control group. Keywords: oligospermia.0 (p<0. Lingadāha and oligospermia. Due to Ātmaguptādīchūrna mentioned in BhaisajyaRatnāvalī for DhatvādīKshaya. Charaka in Uttaravasthi siddhi mentioned Balādi YāpanaVasti for improvement of oligospermia.4± 83. Keywords: Madhumeha. both therapeutic modalities were similarly effective in the management of diabetes. Oligospermia is the commonest problem identified among male infertility in clinical practice. given the proper action by a small quantity and as a special Vasti which can be given without special restrictions.13(p<0.6± 26. Seminal parameters were compared as well as Kshīnashukra symptoms and sexual health parameters of selected oligospermic patients to evaluate the efficacy of the treatments.68 (p<0.01) and combined therapy from 131.1±38.42 to 227. 6± 5.95(p<0.49(p<0. Among 50% of infertile couples.8±30. Gampaha Wickramarachchi Ayurveda Institute University of Kelaniya.35 to 186. Therapeutic evaluation was done before and after. Sri Lanka Globally.8±52.5±8. But the combined therapy significantly reduced Blood urea from 19.53 to 160.13 to 157.27 to 103. This study concluded that Vasti treatment have a significant effect (p <0. oral use was used for comparison. Yoga. male infertility. LDL was significantly reduced by drug therapy from 117. Both groups showed a statistically significant improvement in sexual desire.15± 6.17(p<0.55(p<0.05).52 to 17. Kshīnashukra.01).62±29.42±4.61 (p<0. Data analysis was done by using SPSS16. sexual health 28 .6±60.1to 172. LDL/HDL was significantly reduced only by drug therapy from 2. Ocimum sanctumLinn. In addition. 3 ± 6.05). the MātrāVasti treatment is effective for Vrushana Vēdana.01) and combined therapy from 195.. MātrāVasti was always considered as Vrishya.51(p<0.01). Oligospermia known as Kshīnashukra is interpreted as reduction in the quantity of sperm to 20 mil/ml or lower.Heenmaduruthalā OP 02-13: A comparative clinical study on the efficacy of balādimāthravasti and ātmaguptadīchūrna in oligospermia (kshēnasukra) WARP Weerasingha.05). HP Wakkumbura Department of Kaumārabrutya and StriRōga.001) and combined therapy from a204. erection and ejaculation. To conclude. market samples OP 02-15: Evaluation of the effect of selected Kashaya from VaraYogasaraya in the management of hyperlipidemia KPDC Perera. KGC Dissanayaka Department of Chikitsa. 10mm and 13mm respectively when inhibitory zone standard is 18mm. 15mm. T2. 13mm respectively when inhibitory zone standard was 22mm and for MRSA in samples T1. Several modern drugs are available for the management of Hyperlipidemia. Keywords: wound healing. but it has shown many side effects. EDC Karunarathne4. T2. and T6 against common wound causing isolates of Staphylococcus aureus. T5 and T6 were as 8mm. T4. Therefore. 10mm. T4. Faculty of Medical sciences. for Pseudomonas aeruginosa in samples T1. 16mm respectively when inhibitory zone standard was 40mm. in triplicate discs of modified agar well diffusion method was adopted and the efficacy of each brands were assessed in comparing with positive control as Amoxicillin for bacteria and fluconazole for fungus. 9mm. T3. University of Kelaniya. oil preparations. According to WHO Global Health risks report. Pseudomonas aeruginosa. increased abnormal cholesterol levels identified as 6th leading risk factor among the 19th leading risk factors attributed to the deaths (CDC. T3. The study was focused to determine antimicrobial effects of six wound healing oil samples in different brands – T1. 15mm. Sri Lanka 4 Gampaha Wickramarachchi Ayurveda Institute. Gampaha Wickramarachchi Ayurveda Institute University of Kelaniya. KM Chandimal3. Sri Lanka Hyperlipidemia is a medical condition that denote raised serum levels of total cholesterol. Hence the objective of this study was to investigate the antimicrobial activity of commercially available wound healing oils in the market. low density lipoprotein cholesterol. Gampaha Wickramarachchi Ayurveda Institute.OP 02-14: Antimicrobial activity of commercially available wound healing oils in Sri Lanka WGNS Samanweera1. 10mm. antimicrobial effect. Sri Lanka 2 Department of Medical education. the need of novel antibiotics for effective treatment for wound infection is necessary in order to eliminate the threat of the infections. T3. for Candida albicans in samples T2. T4. University of Kelaniya. Many Ayurveda herbal products effectively manage the hyperlipidemia without side effects. Candida albicans and Methicillin-Resistant Staphylococcus aureus (MRSA) from laboratory specimens. T5 and T6 were as 12mm. T5 and T6 were as 17mm. T5. 11mm. T2. The study demonstrated that the tested wound healing oil preparations had different efficacies while some oils were ineffective in treatment against common wound causing bacterial species. DI Uluwaduge2. This has reduced the efficiency in current treatment regimens. triglycerides or both total cholesterol and triglyceride. T5 and T6 were as 10mm. 2013). 9mm. With 29 . Gampaha Wickramarachchi Ayurveda Institute. The mean inhibitory zone for Staphylococcus aureus in samples T1. Sri Lanka Wound causes an increasing burden to healthcare systems as the availability of drugs capable of stimulating the process of wound repair is still limited due to the emergence of antimicrobial resistance bacteria. University of Sri Jayewardenepura. ADH Sudeh4 1 Department of Dravyaguna. University of Kelaniya. 11mm and 11mm respectively when inhibitory zone standard is 15mm. Sri Lanka 3 Department of Basic Principles. The antimicrobial sensitivity test. Seventeen among 22 (77%) patients who were only on HSPF and/or STRV had improved FBS. S Sonnadara3. cholesterol. Selected kashaya from varaYogasaraya showed reduction in Mid Arm Circumferences (P < 0. Matara. BMI (P < 0. Sri Lanka 3 Dissanayake Ayurveda Hospital. MP Batagoda6. Diagnostic criteria were made based on serological investigation and Anthropometric measurements before the treatments and after the follow up period.05 was taken as the level of significance. Sri Lanka Routine Western Management (RWM) of Diabetes includes restriction of sugar. Sri Lanka 4 Ayurveda Central Dispensary.A multi-center case study JSA Pathirana1. P Senanayake5. Kahantota Road. Galle. Kurundugahahathapma.05) significantly.chemically grown STRV. Traditional rice based Hela Suwaya Special Porridge Formulae (HSPF) produced of non. Sri Lanka 8 Food Production National Programme. Patients with Gestational Diabetes were also included. Presidential Secretariat. Malabe.112/D. Based on the present study the kashaya selected from varaYogasaraya effectively managed hyperlipidemia without any unwanted effects. Nawungala. Sri Lanka 5 No. RMM Niroshana2. fat (total and saturated) and salt while promoting fiber. Sri Lanka 6 District General Hospital. 1966) in the management of hyperlipidemia. micronutrient and antioxidant (FMA) rich food. SP Saman Kumara4. Sri Lankan traditional rice varieties (STRV) are rich natural sources of FMA. this clinical study was designed to understand the effect of selected traditional kashaya from varaYogasaraya (Gnanavimala.this background. Patients were treated with selected kashaya from varaYogasaraya for 2 weeks. LDL (P < 0. Total cholesterol (P < 0. RDSS Wickramasinghe7. 30 patients were randomly selected according to the inclusion and exclusion criteria. spices and green leaves through indigenous Hela Nila Wedakama (HNW) based food technology have synergistically high FMA compared to STRV alone. Keywords: hyperlipidemia. HSPF/STRV was prescribed as the sole or complimentary therapy in addition to allopathic and/or HNW treatment as a multi-center case study. Ayurveda OP 02-16: Effect of non-chemically grown traditional rice based food in management of diabetes mellitus . triglyceride.05). Gampaha. Preliminary evidence shows that complimentary or sole therapy with HSPF and/or STRV have significant long-term impact on glycaemic control 30 .05). Data were analyzed using SPSS statistical package (paired sample t.05). This is a randomized clinical trial carried out at OPD and IPD of Gampaha Wickramarachchi Ayurveda Institute. KS Dahanayaka8 1 Ayurveda Central Dispensary. Sri Lanka 7 District General Hospital. traditional medicine. Subjects (123 cases) were followed up 4-16 months with Fasting Blood Sugar (FBS). Eighteen out of 18 (100%) patients who followed combination of HNW+ RWM+HSPF and/or STRV had improved glycaemic control. Only two out of 17 patients (12%) who followed RWM alone had reduced FBS. Sri Lanka. Forty- eight from 60 (80%) patients who followed HSPF and/or STRV while on RWM were found to have decreased FBS. The aim of this case study was to study the effect of HSPF on diabetes. Some followed only RWM.05) and elevation of levels of HDL (P > 0. Sri Lanka 2 Ayurveda Hospital Polwatta.test) and P< 0. 95% of the respondents’ appetite was improved. anorexia. Sri Lanka Dengue is a mosquito borne viral infection. headache. Due to this post dengue effects person’s usual life activities and 31 . Dengue fever (DF). under arms. 80% got 4-5 kg weight reduction. and back side as well as double chin.Agni Dīpana and Srōtas śodhana. MJ Fazmiya. and blood pressure was measured and other difficulties were also considered. Results show that the Vāṣpa sweda can be used as for Āma pācana. Sri Lanka This study was done with reference to the basic principle. Keywords: Vāṣpa swēda. fiber. Hela Nila Wedakama OP 02-17: A qualitative study on Vāṣpa Sweda used for detoxification of Āma as one of the rejuvenation treatments D Premathilake District Ayurvedic Hospital. Keywords: Sri Lankan traditional rice. This study was done to identify the effectiveness of Vāṣpaswēdafor dispelling Āma. Āma. antioxidants. Before and after the treatment. Controlled clinical trials are being designed for further proof. scaling of epidermis in palms and soles. The best glycaemic control is achieved when HNW is coupled. VSKR Senaka Bandaranaike Memorial Ayurveda Research Institute. myalgia. and weight loss etc. Overall observations conclude that Vāṣpa swēdais an effective and efficient therapy which can be done to maintain good health without any drug intake. micronutrients. a by-product of improper digestion. body mass Index. buttocks. 20% got less than 2 kg weight reduction. 90% stated that their joint pains and muscle stiffness were reduced after the treatment. thus.compared to RWM alone. breasts. which leads to Āma. 60% of the respondents stated that their bowel movements were stabilized. W Weerapana. humours OP 02-18: Survey on post-dengue effects MRS Peiris. at Makuddala Ayurvedic Hospital in Kegalle District. Acute symptoms of dengue subside within few days. Dengue Haemorrhagic fever (DHF) and Dengue Shock Syndrome (DSS) are three clinical types. 95% of the respondents had receded flabby muscles in abdomen. “Āma” is undigested food mass.60 years. the root cause of all diseases is impaired Agni. 95% stated that their congestion of phlegm and URT problems were reduced and breathing difficulties were reduced after the treatment. fatigue. Accumulated undigested food combines with Humours which become vitiated. dyspnoea. Āma pācana. all patients’ body weight. Srōtas śodhana. arthralgia. Agni dīpanaand Srotas śodhana. chest pain. the results show that Vāṣpa swēda rejuvenates the body without inserting any drugs. Further. thighs. After a 7 day treatment.The objective of the study was to determine the efficacy of Vāṣpasweda for Āma pācana. Purposive sample of 100 overweight females were selected of age group 20 . Meerigama. Agni dīpana. who attended the overweight clinic. 98% gained a healthy glowing and young appearance and skin discoloration also was reduced after the Vāṣpa sweda. According to the Ayurvedic concept. It was stated that fatigue was reduced in 90% of the respondents. some patients experienced post dengue effects such as hair loss. RDH Kulathunga2. myalgia. Sri Lanka and divided in to two groups. Institute of Indigenous Medicine. It concludes that majority of participants complained post dengue effects of hair loss. Keywords: dengue. obesity. with no significant differences in demographic or clinical characteristics. scaling of epidermis in palms and soles. Keywords: Daśāṅga Guggulu. hepatic and renal functions. and arthralgia for period of one to two months. Majority of 91% patients showed post dengue effects whereas 9% not shown any. It identified that arthralgia. Tripalā Cūrna. Furthermore both drug regimens caused significant mean reductions from baseline of SGOT. 75% participants got dengue fever. anorexia. BMI. Sri Lanka and Welagedara Ayurvedic Hospital Kurunegala. SGPT.05). Sri Lanka 2 Department of Kayachikitsa. 23% had scaling of epidermis on palms and soles and. who were at a period of 12 weeks after recovery at the time of survey. loss of hair. dengue haemorrhagic fever. Serum creatinine and blood urea nitrogen (P < 0. headache as post dengue effects (PDE). Daśāṅga Guggulu 1g and Tripalā Cūrna 5 g were distributed three times a day for Group I and Group II respectively for three months. Total number of 60 obese subjects (Body Mass Index (BMI)>25kg/m2) were randomly selected at a Kāyacikitsā clinic. Ayurveda Teaching Hospital Borella. In conclusion. The two treatment groups were well matched. both drugs were effective in reduction of BMI and appeared to be safe and were well tolerated.001) and there was not a significant mean difference between two groups. Daśāṅga Guggulu and Tripalā Cūrna showed highly statistical significant mean reduction of BMI (p<0. Sri Lanka 3 National Hospital of Sri Lanka Daśāṅga Guggulu and Tripalā Cūrna are the most valuable drugs in management of obesity. Data collected form hundred respondents who got dengue infection. Others 16%. DUS Rathnapala3 1 Department of Kayachikitsa. University Of Colombo. weight loss. Participants included above 15 years of age suffered dengue infection and confirmed it by specific laboratory investigations also. Among group 53% of experienced hair loss.general health get disturbed. dyspnoea. Results revealed. The aim of this study was to compare the efficacy and safety profile of Daśāṅga Guggulu and Tripalā Cūrna using BMI. University of Colombo. with no significant differences between the two groups. chest pain and dyspnoea respectively. fatigue. fatigue. Participants interviewed by using a prepared questionnaire. A cross-sectional survey carried out to identify post dengue effects. post-dengue effects OP 02-19: Clinical assessment of efficacy and safety profile of Daśāṅga Guggulu compared with Tripalā Cūrna in management of obesity JMGSL Jayasinghe1. chest pain. kidney function and liver function tests were assessed before and after the treatment and paired and unpaired t tests were applied to analyze the data. 9% and 6% suffered myalgia. Thirty-three percent (33%) complained of anorexia. 23% dengue hemorrhagic fever and 2% developed dengue shock syndrome. 18% reported of having headache. safety profile 32 . Institute of Indigenous Medicine. 52% of each had arthralgia and fatigue. as per the cikitsā sūtra (line of treatment) in classical texts. It was conducted at Bandaranaike Memorial Ayurveda Research hospital. it can be concluded that the treatment protocol followed to treat the patient was effective in the management of Gaja Carma. All patients received a standard SLTM regime prescribed by qualified Ayurveda doctors. it was not managed at all. KDPM Weerasinghe. W Abeyrathne3 1 Department of Pre-Clinical Sciences. Although he was treated previously with allopathic treatments from the age of 5 years. face and scalp areas for more than 12 years. ample eruption. Ayurvedic management. Hence. roughness of the skin in limbs. University of Colombo. General Sir John Kotelawala Defense University. chest. itching. Sri Lanka 4 Institute of Indigenous Medicine. Venous blood (3ml) was collected at the outset (1st week) and 11 follow up clinics were conducted at 3 week intervals to measure the variation of serum creatinine level with treatments. 33 . LUP Liyanage Bandaranaike Memorial Ayurveda Research Institute. General Sir John Kotelawala Defense University. Nawinna. Faculty of Allied Health Sciences. Epidermolytic hyperkeratosis OP 02-21: The efficacy of Sri Lankan traditional medicine on patients with Chronic Kidney Disease of unknown etiology in Sri Lanka N Kumarasinghe1. back. C Karunarathna5. Keywords: Gaja Carma. Faculty of Medicine. Sri Lanka Chronic kidney disease of uncertain aetiology (CKDu) mainly affects North Central Sri Lanka. Here we present continuous follow up serum creatinine data derived from a group of 121 CKDu patients receiving Sri Lankan Traditional Medicine (SLTM) treatments offered at the Government Ayurveda Hospital Kebithigollewa. He was administrated with Ayurvedic medicine for one and half month. Histopathology shows tubular and interstitial fibrosis along with tubular atrophy. P Jayawardana4. Panadura. internal snehana (oleation). Sri Lanka 2 Department of Medical Laboratory Sciences. Sri Lanka 3 Institute of Ayurveda and Alternative Medicine. Sri Lanka Ayurveda management of Gaja Carma in correlation to epidermolytic hyperkeratosis is a vulnerable and rare skin disorder described under Kuṣṭha in Ayurveda. GLS Galgamuwa2. After one month period there was a marked improvement observed with reduced signs and symptoms from the normal state. It can be correlated with epidermolytic hyperkeratosis which is characterized by redness and severe hyperkeratosis with small.OP 02-20: Case study: Ayurveda management of Gaja Carma in correlation to epidermolytic hyperkeratosis V Seneviratne. This study describes a case of a 14 year old boy treated in the inpatient department. hard wart-like scales over the entire body. It was based on vāta-kapha śamana cikitsā (doṣa pacification) and multiple śodhana methods (detoxification / purifying). characterized by roughness. Treatment protocol included local and systemic therapies. K Jayasekara2. vasti (enema detoxification) and specific local applications. I Abeykoon1. This study was aimed to observe the efficacy of Ayurveda treatment modality in the management of Gaja Carma. P Kulanatha4. V Suriyakumara3. similar to elephant skin. U Siriwardene3. who presented with blackish discolouration. Agnimanthachurna has been found to reduce the serum cholesterol level from185. Every patient who underwent treatment was subjected to necessary laboratory investigations including Lipid profile and BMI.07 by -4. Sri Lankan Traditional Medicine.9 ± 10. angina pectoris. data was analyzed by Paired t-test.228). Institute of Indigenous Medicine. Sri Lanka Obesity (Staulya) is one of the common nutritional disorders in affluent societies.021 and female.22%. initial LDL level was decreased by 6. initial triglyceride level was decreased by16. Keywords: Chronic Kidney Disease of uncertain etiology. serum creatinine OP 02-22: Clinical study on the efficacy of Agnimantha churna (Premna intergrifolia Linn) in the management of obesity KPMP Pathirana1. Sri Lanka 2 Department of Desheeya Chikitsa. Keywords: Staulya. Survey study was done among 30 obese patients and 15 obese patients were subjected to the clinical study who attended Gampaha Wicramarachchi Ayurveda Hospital. but the values were statistically insignificant (p>0. p=0. but again the value was insignificant.a survey and clinical study. This study showed a significant reduction in serum creatinine throughout the period of 37 weeks.80 to 36. Gampaha Wickramarachchi Ayurveda Institute. Difference between 1st and 6th clinic (19 weeks) creatinine levels were not statistically significant (male. In the survey. Initial BMI decreased from 36. which is also insignificant. p=0. Gradual decrease of mean creatinine levels was observed in both gender groups during 12 consecutive clinics.26%.097 and female. The aim of the study was to identify the efficacy of Agnimanthachurna in the management of obesity. Currently clinical. hypertension. p=0.BMI.14 to 42. From the above study it may be concluded that Agnimanthachurna has some beneficial effect of lipid profile and BMI. p = 0. LDL. University of Colombo. observed in patients receiving SLTM in Kebithigollewa.79 to181. HDL 34 . Agnimantha (Premna intergrifolia Linn) is well known to have Meddohara. lifestyle and other related factors by specially prepared survey pro forma.024). 5g two times a day with warm water before meals.64 mg/dl (4.7 years) and 43 females (57. myocardial infarction etc is higher among obese individuals.93%. In the clinical study. The incidence of diabetes mellitus. Agnimantha churna. epidemiological and quality of life data are being analyzed and will be presented at a later date. obese individuals were surveyed for their diet. This study had two aspects . Lekhana.15%). University of Kelaniya. Serum HDL level was increased from 37.05).4±8. however a significant decline was observed between the 1st and 12th clinic (37 weeks) in both genders (male. Selected patients were treated for two months with Agnimanthachurna.54 Kg/m2 by 0. Shothahara and Krimighana properties. SMS Samarakoon2 1 Department of Dravyagunavignana. Finally.43%.The group comprised of 78 males (59.5 years). University of Kelaniya. Sri Lanka 2 District Ayurveda Hospital Panvila. Seethodaka. JAAS Jayaweera3 1 Bandaranaike Memorial Ayurveda Research Institute. Pipe chaba. Pinda. extension.035) improvement. Sri Lanka 2 Gampaha Wickramarachchi Ayurveda Institute. fracture healing.OP 02-23: Healing of non-union compound fracture of tibia by traditional remedies -A case report AMHS Attanayake1. AP and L. as well as sensory and motor function assessment was performed to monitor the fracture healing. The objective of this study was to assess the fracture healing effect of Sri Lankan traditional medicine for the non- union of compound fractures over shaft of tibia following internal fixation failure. while the contour of the leg became normal over the six months period of treatment showing significant (p=0. Sri Lanka Chronic allergic rhinitis is a very common disease with the cardinal symptoms of nasal itching. The patients were selected from the outpatient Department of Ayurveda central dispensary. Sri Lanka according to the selection criteria and divided into Group A (n=30) and Group B (n=30). two times per day before meals for 30 days. in the management of chronic allergic rhinitis. He was followed over 18 months of allopathic treatment with an internal fixator and another 6 months with external fixation with Plaster of Paris. oedema and local tenderness decreased by 90%. Pain. Nawalapitiya.X-ray. Sri Lanka 3 Faculty of Medicine and Allied Sciences. Further. Narayana oils and paste of Katakaladi was applied during the treatment. Patients of Group B were treated with 35 . Nawalapitiya. KGC Dissanayake2 1 Ayurveda Central Dispensary.ray showed delayed-union with no evidence of callus formation. Piper longum. UMGD De Silva2. After attending Ayurveda treatment. A follow-up large sample study will be important to assess the efficacy of given traditional treatment protocol and management procedures. Sequentially. Splints were kept on the lateral and medial side from ankle joint to hip joint. non-union compound shaft tibia fracture OP 02-24: Study on the effects of selected herbal formulation derived from VaraYogasāra in the management of chronic allergic rhinitis PHAN Piyasinghe1. Phyllanthus emblica. At about 6 months. Over 2 years he failed to recover (non- union). The primary objective of this randomized clinical trial was to determine the effect and efficacy of herbal formulation derived from ola leaves manuscript named as VaraYogasāra. The antero-posterior (AP) and lateral (L) X. Patient started to walk after 6 months of successful Ayurveda treatment. A twenty-eight year old male attended Ayurveda treatment following non-union of compound fracture over shaft of right tibia. muscle power. Zingiber officinale derived from VaraYogasāraya at a dose of 120ml. flexion and rotation of the leg were also improved significantly (p=0. Rajarata University of Sri Lanka  Shaft of the tibia fracture is common and associated non-union is often complicated.  Keywords: Ayurvedic treatment. rhinorrhea. and nasal congestion. the fracture was immobilized using bamboo splints over a period of 6 months. X-rays showed  callus formation (80%) with increasing thickness (75%) and remolding (75%) of the bone fragments.04). WAL Chandrasiri2. Patients of Group A were treated with the decoction of selected herbal formulation consisting of Terminalia chebula. sneezing. which showed no significant improvement after 30 days except for rhinorrhea. Scientists believe that the disease is due to an autoimmune disorder.e.lifestyle and dietary management. MAG Madusanka3 1 Department of Shalya Shalakya. which is an indicator that it is possible to stimulate the melanocytes to produce melanin. Perifollicular and diffuse type re-pigmentation was noted significantly. Further. most of the selected cases showed perifollicular and diffuse type of pigmentation.2 % of the world population and is cosmetically and psychologically devastating. sneezing. babchi (psorolea corylifolia). University of Kelaniya. VaraYogasāra. with other specific clinical features. nasal obstruction. Photograph of the lesions were taken before the treatment and every two weeks. After the formation of blisters the white patches turned dark. University of Kelaniya. Keywords: Vitiligo. With its symptomatology it can be correlated with Chronic Suppurative Otitis Media (CSOM). especially in developing countries. patients were treated with strict diet sheet and medicines. Patients were selected as purposive sampling for a qualitative case study. Over 90% of the burden is borne by countries in the South – East Asia and Western Pacific Regions. Patients of Group A showed significant reduction in absolute eosinophil count and clinical symptoms i. melanin OP 02-26: The anti-microbial effect of Karna dhupana Yoga against Puthikarna (Chronic Suppurative Otitis Media) KPP Peiris1. Africa and several ethnic 36 . Sri Lanka Vitiligo is a chronic hypo-melanotic skin disease of unknown etiology affecting 1. These results suggest that the selected herbal formulation is effective in the management of chronic allergic rhinitis and provide scientific background for further studies. Sudesh ADH2. re-pigmentation. Gampaha Wickramarachchi Ayurveda Institute. There is currently no satisfactory treatment package to cure this condition. Formation of blisters was noted in 60% of patients. This study aimed to assess whether it is possible to stimulate the melanocytes to produce melanin by using locally available treatment packages. Ola leaves manuscripts OP 2-25: Advanced evaluation of locally available treatment packages for treatment of vitiligo MA Jaleel National Ayurvedic teaching Hospital. Sri Lanka 2 Gampaha Wickramarachchi Ayurveda Institute. Sri Lanka 3 Gampaha Wickramarachchi Ayurveda Hospital. rhinorrhea and anosmia (p<0. This study consisted of 40 patients who visited the National Ayurvedic Teaching Hospital skin clinic OPD and the diagnosis was essentially clinical. It is a major cause of acquired hearing impairment in children as well as adults. Results show re-pigmentations were noted within two weeks and a month of the treatment. Keywords: allergic rhinitis.001) after 30 days in comparison to those of Group B. The photographs of the lesions were compared with the photograph taken before treatment. Sri Lanka Putikarna is a chronic disease caused by vitiated Kapha and Pitta Dosha with its characteristic features of Puti and Puya. and all three gave similar results of no growth after 30 mins of incubation. Organic extracts prepared from whole plants of P. G Wijayaratne5. sesquiterpenes. The MIC values observed as either 31. Germany 4 Research Group Biosynthesis/NMR.A potent antimicrobial activity was observed in dichloromethane extract with minimum inhibitory concentration (MIC) in the range of 62. acyclic diterpenes and several other metabolites. zeylanicus and to characterize the bioactive phytochemicals thereof. CSOM produces chronic mastoiditis by contiguous spread to erosion of the walls of the middle ear and mastoid cavity. Sri Lanka 2 Natural Products Research Group. Max Planck Institute for Chemical Ecology.minorities in the Pacific rim. CSOM OP 02-27: Plectranthus zeylanicus: A potent source of antimicrobial phytochemicals from tropical flora M Napagoda1. which in turn can lead to such complications as facial nerve paralysis. Keywords: Karna Dhupana. Faculty of Medicine. the pharmacological features and the phytochemistry of this plant hasnot been comprehensively explored yet to rationalize the reported ethnobotanical significance. revealing the presence of phytosterols. University of Ruhuna. Max Planck Institute for Chemical Ecology. meningitis and brain abscess. L Jayasinghe6 1 Department of Biochemistry. However.25 or 62. A Nagahawatte5. National Institute of Fundamental Studies. This compound was extensively studied for its antimicrobial activity using clinical isolates including nine strains of Methicillin-Resistant Staphylococcus aureus. thepresent study was undertaken to evaluate the antimicrobial potential of different extracts of P. This was done as triplicates. The study was carried out at the central laboratory using a specially prepared fumigation instrument.5 µg/mL were significantly better than the MIC values reported in 37 . Ma Qader2. National Institute of Fundamental Studies. Sri Lanka 6 Natural Products Research Group. According to the Ayurvedic system of medicine Puti Karna can be managed by a very effective local treatment modality known as Karna Dhupana. S De Soyza1. labyrinthitis. Most approaches to its treatment have been unsatisfactory or are very expensive and difficult. the structure of the most active compound was elucidated as 7α-acetoxy-6β- hydroxyroyleanone by liquid chromatography coupled mass spectrometry and nuclear magnetic resonance data.5-125 µg/mL. A Svatoš3. Thus this study concludes that Karna Dhupanachurna has an antibacterial activity against Staphylococcus aureus (ATCC 25923) and thus is likely to be effective on Puti Karna (CSOM) that is caused by Staphylococcus aureus (ATCC 25923). Fumigation was done by using Karna Dhupanachurna to the nutrient agar media for 10 minutes and 30 minutes as two trials. hence subjected to the activity guided fractionation. The extract was also analyzed by gas chromatography coupled mass spectrometry. Therefore. Among the compounds isolated from this extract. Sri Lanka Plectranthuszeylanicus (Family: Lamiaceae) is a perennial herb extensively utilized as an antimicrobial remedy in traditional medicine in Sri Lanka. Staphylococcus aureus and Staphylococcus saprophyticus by disc diffusion and broth micro-dilution methods. Germany 5 Department of Microbiology. the present study was carried out to determine the antibacterial activity of Karna Dhupanachurna using nutrient agar media against Staphylococcus aureus (ATCC 25923). University of Ruhuna. lateral sinus thrombosis. S Lorenz3. Puthikarna. Faculty of Medicine. zeylanicus were tested against Enterococcus faecalis. Therefore. Sri Lanka 3 Research Group Mass Spectrometry and Proteomics. B Schneider4. 0. Keywords: Adenanthera pavonina stem. Plectranthus zeylanicus. Methanol and aqueous extracts were evaluated for antioxidant activity using DPPH method with BHT as the standard and anti-inflammatory activity using Red Blood Cell Membrane Stabilizing assay method with Asprin as the standard. Keywords: antimicrobial. University of Kelaniya. P Paranagama2.01.08 ± 0. glycosides.respectively. University of Kelaniya.inflammatory agent use in Sri Lankan Traditional Medicine APA Jayasiri1.03 µg/mL respectively while anti-inflammatory activity of both methanol and aqueous extracts and standard were 0. chloroform.03. Results of both activities were expressed as IC50 values using Probit Analysis Statistical software. Therefore. methanol and water.49 ± 0.23 and 0. secondary metabolites. 50. Results can be concluded that AP can be use as natural source of antioxidants due to presence of above phytochemicals and act as membrane stabilizer that capable of providing alternative source for management and treatment of inflammatory related disorders and diseases.literature for most of the ubiquitous phyto-constituents. the present study suggests that AP would serve as a source for the discovery of novel anti-inflammatory agent. but its biological validity was not evaluated. Qualitative phytochemical analysis was done to identify the chemical compounds present in the stem bark extracts of different solvents hexane.14 ± 0. Plectranthuszeylanicusis a potent source of bioactive phytochemicals and further studies on biological activities and self-assembly properties of the isolated secondary metabolites are in progress. APG Amarasinghe1 1 Institute of Indigenous Medicine. The results revealed antioxidant activity of both methanol and aqueous extracts and standard were 51. chloroform and water extracts were positive for phytochemicals of alkaloids. Sri Lanka Adenanthera pavonina (AP) is a medicinal plant belongs to family Fabaceae use as an ingredient in different traditional drug preparations identified as Madatiya or Madati Mara by Sri Lankan traditional medical practitioners. steroids and saponins. University of Colombo. chronic ulcers.12 ± 0. Sri Lanka 2 Department of Chemistry. flavonoids. SP Senanayake3.02 and 55. It is a commonly include in drug preparations use in treat abscess. Sri Lanka 3 Department of Botany. bark aqueous extract. antioxidant 38 . tannins.12 ± 0. The study confirmed methanol. Methicillin-Resistant Staphylococcus aureus OP 02-28: Biological evaluation of Adenanthera pavonina (Madati Mara) as a source of antioxidant and anti.01 µg/mL.07 ± 0. The standardized gotukola powder and capsules were manufactured as per the pharmacopeial standards at a GMP certified premises. Dushtavrana is a long standing ulcer with profuse discharge and slough. cow’s milk.A randomized controlled clinical trial (RCT) VPS Seneviratne1. It was evaluated by Montreal Cognitive Assessment (MOCA) tool. calculations. In comparison of two groups. The Voucher speciman was authenticated at the Herbarium at Bandaranaike Memorial Ayurveda Research Institute. attention and concentration. Ministry of Health and Indigenous Medicine. It was well documented in almost all Ayurveda textual references. University of Colombo.00) statistical software. Yakkala. language/verbal fluency. Ethical clearance was obtained for the study from ERCIIM (ERC 14/34). mean value of gotukola with cow’s milk group was showed significant improvement in executive functions/skills. Keywords: Centella asiatica. The research design was an open label.1). Sixty volunteer individuals were selected randomly and sub-divided into two groups (n=30). attention and concentration. One group was given gotukola capsule once a day and the other group was given gotukola capsule with cow’s milk once a day. A lady of 52 years presented to the OPD of the hospital with a large non-healing wound on the plantar surface of the left foot since 30 years. memory. randomised controlled clinical trial. SD Hapuarachchi2. 39 . According to the results. Sri Lanka 3 Faculty of Medicine. it should be protected from Doshadushti and from various micro-organisms. cognition OP 02-30: Management of Dushtavrana with selected Shashtivida upakrama – A case study KCP Gunarathne Gampaha Wickramarachchi Ayurveda Hospital. The main objective of the study was to assess the effectiveness of Gotukola capsules with cow’s milk in increasing selected cognitive abilities in adults (16-40). P de Zoysa3 1 Ayurveda Research Institute. the mean value of the visuo- constructional skills. abstraction and orientation were increased significantly in the group treated with gotukola capsules in combination with cow’s milk (p<0. memory. abstraction and orientation. Sri Lanka The history of medical science commenced with the art and skill of wound healing and it has prime importance in Surgery.OP 02-29: Effects of Gotukola (Centella asiatica) capsules with cow’s milk in modulation of cognition in young adults. healthy granulation tissues also can be damaged. Both groups were followed up for three months and were observed for selective cognitive functions. University of Colombo. executive functions/skills. conceptual thinking.01). (Version 7. language/ verbal fluency. conceptual thinking. Acharya Sushruta mentioned Vranavinishcayārtham as a great scope of Shalya tantra. it can be reported that gotukola powder has significant effects on selected cognitive functions by adding cow’s milk. Sri Lanka 2I nstitue of Indigenous Medicine. Though healing of Vrana is a natural process of the body. The statistical analysis was conducted by paired t-test and pooled t-test by using SPSS (Version16. They can be cleaned by using surgical instruments or oxidizing agents. calculations. After three months follow-up of two groups. By that. Sri Lanka Centella asiatica (Gotukola) is one of the potential medhya rasayana (intellect promoting) medicine identified in ancient system of Ayurveda. PK Perera2. naming. 878. SD=4. Sri Lanka Dementia is a combination of several symptoms that are associated with the declining abilities of the brain and its functions.920). SD=0.716).05 . Various Thaila (oils). University of Kelaniya. (M= 18. agitation.983. memory. Shoola (Pain). combination of treatment models while managing infected or chronic wounds should be used.679). p<0. SD=3. VranaDhara.10. SD=0.25. Moreover. language skills. S-MMSE.822) (M= 0.50. A non-randomized. HA de Silva3 1 Institute of Indigenous Medicine. Sri Lanka 3 Faculty of Medicine. SD=0. BPSD.t(39) 3. SD=0. retrospective. (M= 0. 8 mg of Sārasvata Cūrnain divided doses was given for three months.05. More than 30 million people worldwide are currently living with dementia.t(39) 3. SD=0. sizeand depththan when using only normal simple dressings.65. SD=0. Results of the before and after treatment were analyzed by using paired sample t test for the observational study on scores for total of S-MMSE.777.05. (M= 0.52. SD=2.871) (M= 0. (M= 18.736). A high dementia prevalence of 4% has been reported from a regional community survey in Ragama. SD=0. Behavioral and Psychological Symptoms of Dementia (BPSD) were assessed. Avagaha (dipping). p<0.50.05. dementia 40 .736) (M= 0. Activities of Daily Living (ADL) Index. Keywords: Dushta Vrana.35.22. Literature of Ayurveda has mentioned that impairment of memory as Smṛtibraṁśa and Sārasvata Cūrnais a commonly used formulae for impairment of memory. University of Kelaniya. understanding and judgement. ADLI is stable. SD=0. Sri Lanka 2 Faculty of Humanities. Primary data of 40 patients with memory impairment (mild to moderate) on the basis of a questionnaire and Mini Mental State Examination-Sinhala (S-MMSE). Avagaha. p<0. apathy). Vrana Basti.05 respectively. SD=2.05. burning and itchiness. ADLI. Vranadhara (poring). BPSD (delusion.439). as well as80% faster reduction of wound discharge. for six months. In conclusion.781. For Vrana management. Smṛtibraṁśa. Vranabasti (stagnant) and Pathradharana (covering with leaves) were used as local treatments with few modifications from Shashti vidhaupakrama of wound management in Susruthasamhitha. pain. ( M= 1. University of Colombo. Sri Lanka. attending the OPD in Ayurveda Teaching Hospital at Borella were selected. p<0.716). Pathra (leaves) and Kwatha (detoctions) were used according to the lakshana (symptoms) of wounds. (M= 22. The advantages of these procedures were relief of.associated with moderate Daha (Burning).235). Keywords: Sārasvata Cūrna.325). Shashti VidhaUpakrama OP 02-32: An observational study of efficacy of Sārasvata Cūrna in the management of Dementia (Smṛtibraṁśa) TDN Karunaratne1. K Sugataratana2. This case shows that for fast healing with minimum suffering. one group pre-test-post-test study design was adopted. cognition. before and after treatment.55. (M= 1.496).652.88. (M= 19.65. t(39) -0. The objective of this study was to evaluate the efficacy of Sārasvata Cūrna in the management of Dementia. more than 70% were of the Alzheimer type. Sārasvata Cūrnacan be used in the management of dementia. mean change of scores indicated that. The wound was not infected and healed with minimum complications. repetitive behavior. there was a significant effectiveness of before and after treatment of Sārasvata Cūrna. HAS Ariyawansa1.32. The findings showed that. Kandu (Itching) and Srava (Discharges from wound). There may be a decline in thinking. t(39) 6. t(39) 4. p<0. t(39) -3. Among the people over 65 years. p<0.766. (M= 0. There was a significant difference in the total score of MMSE. Keywords: Muscular Dystrophy.2 ± 0. Therefore.05).OP 02-33: A clinical study of the efficacy of an Ayurvedic treatment regimen for Duhenne Muscular Dystrophy (Mansagatha vattha) N Dadallage National Ayurvedic Teaching Hospital.025). T2 and T3) were fed 15g/hen/dayof fresh I. We investigated whether dietary addition of Ipomoea aquatica (kankun). Alternanthera sessilis and Pennisetum purpureum x Pennisetum americanum AGH Ranasinghe. americanum(T3) together with regular feed (115g/hen/day). It is a progressive and resulting in death in the early twenties. purpureum x P. TJ Withanage. CPK level (p=0. Eggs from mukunuwenna (8. On investigation the CPK (Creatininephosphokinase in serum) levels are elevated at least 10 times the normal level. and difficulty in climbing steps. They are frequent falls. sessilis (T2) and P. Symptoms appear between the ages of 4 – 6 years of age. Faculty of Veterinary Medicine and Animal Science. stiffness of calf muscle (p=0. Paired t-test was used to analyze the data and statistically whcihch showed significant results (circumference of calf muscle (p = 0. carotenoids were quantified by spectro-photometry and results were analysed using an ANOVA with post-hoc comparisons. Eggs of kankun-fed hens had no difference in carotenoid levels (6. KRCK Lasankara. Following Ayurvedic treatment the symptoms were gradually reduced and muscles became strong. aquatica (T1). Peradeniya. The control group was fed a layer diet of 115g/hen/day. N Jayasena. 14 and 21 days.2 µg/g) did not increase with time (P>0. Usually boys are affected by this disease. Sri Lanka Carotenoids increase the colour of egg-yolk and have health benefits. Sri Lanka Duchenne Muscular Dystrophy (DMD) is the commonest hereditary degenerative neuro-muscular disorder. Alternanthera sessilis (mukunuwenna) and Pennisetum purpureum x Pennisetumamericanum(Hybrid Napier.1 ± 0.05). It is important to consider alternative approach of treatment because of no specific treatment in any medical system in the world. A. Three eggs from each group were collected at 0. could climb stairs and lordotic gait was reduced. Sample size was 50 patients.Shaver Brown hens (N=200. Objective of the clinical study was to investigate the efficacy of an Ayurvedic treatment on DMD patients. children developed a good gait. Mansagathavatha OP 02-34: Deposition of carotenoids in egg yolk by short term supplement of Ipomoea aquatica. lordosis waddling gait. Gover's sign is positive and calf muscles are enlarged and weak. Results showed that carotenoids did not differ between treatments when compared with the control on day 0 (P>0.008).4 µg/g) when compared with control eggs (P>0. University of Peradeniya.05) on days 7. this therapy is considered effective and is recommended for these children. LJPAP Jayasooriya. 7. CO-3) to laying hens increases deposition of carotenoids in eggs. WMAP Wanigasekera Department of Basic Veterinary Sciences.3 µg/g) and CO-3 (10. It is correlated with Mansagathavatha described in Ayurveda. age=55 wks) were divided into four groups (n=50 each). The three treatment groups (T1. Gover’s Sign did not reveal a significant P value. 14 and 21.2 41 .15).7 ± 0. Mean carotenoid content in control eggs (4. Birth is normal but sometimes delayed walking is reported. Keywords: eggs. In conclusion.05). This can increase the nutritional and aesthetic value of eggs. sessilisor CO- 3. Ipomoea aquatica.001).5 µg/g) fed hens had higher carotenoids when compared with control eggs on all three days (P≤0. hybrid Napier. carotenoids.± 0. Alternanthera sessilis 42 . There were no continuous increases in carotenoids in any treatment from day 7-21 (P>0. carotenoids in eggs can be increased by feeding layers with A. Track 03 Recent advances and evidence based practices of traditional medical systems on non-communicable diseases Abstracts of Keynote Speakers and Oral Presenters 43 . Role of Ayurveda in the Management of Tuberculosis. adaptogenic effect. free radicals scavenging and anti-oxidant effect. fever. which can be of immense use for the prevention and management of drug resistant cases. extensively drug- resistant TB (XDR-TB) along with debilitating side effects of anti-TB drugs. The Anti Koch’s Treatment precipitates gastrointestinal upset. skin rash. TB always offers newer challenges such as multidrug-resistant TB (MDR-TB). hepatotoxicity. Rejuvenative Drugs. Low dose Rifampicin (200 mg). Debrecen University. Rasayana. Especially in the Field of Multi Drug Resistant Cases Prof. Tuberculosis is described as an infectious disease and its mode of transmission along with treatment modalities are also described in detail. with encouraging results. early sputum negative results. Rasayanain Ayurveda isused as specific rejuvenative drugs for prevention and management of various diseases. Madhaw Singh Baghel Former Ayurveda Chair. sometimes even seizures. Some of the preparations showed potential hepato-protective properties. Many herbs and mineralometalic preparations have been widely studied in clinical studies conducted by Ayurvedic Institutions within various tuberculosis centres in India. Mineralometalic Drugs 44 . These symptoms may cause discontinuation of therapy. as Piperine enhances the bioavailability of rifampicine and has been marketed as novel. psychiatric symptoms. In spite of noticeable progress achieved. Rasayana Therapy has been studied for its anabolic effect. Rasayana drugs are used to maintain ongoing metabolism. pulmonary tuberculosis hasbeen well described since antiquity and the written documentation has been available for more than 3000 years inCaraka Samhita. bio-inhancer effect and immuno-modulator effect in cases of tuberculosis in-vitro and in-vivo. nephrotoxicity. Keywords: Multi-Drug resistant TB. This gives an insight that with the use of Ayurvedic Rasayana drugs an early recovery. Piper longum is the best example. Further the role of mineralometallic drugs are being investigated from a Nano technology perspective. leading to desired performance of body elements and tissue development. a bio-enhancer Piperine (10 mg) and standard dose Isoniazid (300 mg) was given along with Ethambutol and Pyrazinamide with comparable efficacy with standard WHO therapy using a conventional formulation. resulting in drug resistance. arthralgia. Hungary (Jaipur India) In Ayurveda disease. better compliance of DOTs and better management of MDTR and XLR cases can be managed with the least ADRs. These studies have shown some significant results as adjuvants in preventing adverse drug reactions (ADRs) and giving significant quick recovery and even in some cases. are some of the key mechanisms that brings anti-cancer activities here. and also enhancing immune system. the heterogeneity of cancer biomarker expressions. butstill with many side effects. in particular.and increasing quality of life of cancer patients. These evidence-based applications of herbal medicines in cancer therapy strongly indicates that it is time to integrate traditional treatment systems with current western treatment techniques to achieve much improved patient survival.directly or indirectly targeting cancer cells. and also have skyrocketing prices. more than 90% of all new oncology drugs that enter clinical development do not obtain marketing approval. The Value of the Traditional Medical System on Cancer Prevention and Research: It is Time to Integrate with Western Medical Approaches Dr. Immune system 45 . Recentstudies on the biochemical and cellular level mechanisms of herbal medicines in specific tumor microenvironments provide molecular level evidence of how these treatments have activity. Medicinal herbs used in traditional treatment systems are being increasingly recognized as useful complementary treatments for cancer. limiting their availability to the economically advantaged. Unlike any other disease. Gomika Udugamasooriya Associate Professor. when these herbal medicines are used in combination with current medications. Department of Pharmacological & Pharmaceutical Sciences. Many clinical studies have been conducted on these traditional treatment systems indicatingbetter patient survival.Many of the approved drugs are effective only within selected patient populations.The presence of a large number of active compounds that work together at low concentrations. Traditional treatment. uplifting the immune system. University of Houston Despite extraordinary advances in our understanding of the biology of cancer as well as the identification of molecular targets for treatment. Keywords: Medicinal herbs. presence of drug resisting cancer stem cells and unique mechanisms that tumors use to evade immune system are some of the reasons for failures of these conventional cancer treatments. Cancer. IL- 10 signaling and NFκβ signaling. McGovern Medical School. Genomic and Proteomic Effects of the Anticancer Agent Salinomycin Dr. to gain insight into its mechanisms of action by correlating the temporal genomic. The expression patterns seen at 3 different time points revealed that salinomycin altered the expression of 575 probes at 2 hours. and secondly. at longer exposures. to test the anti-tumoral properties of salinomycin. 1500 probes at 12 hours. 46 . Exposure to salinomycin 5 µM showed classical morphology of apoptosis in over 90% of cells at 24 hrs. The increase in the number of probes altered at the 12 and 24 hour time points suggest more widespread cellular activation. Priya Weerasinghe University of Texas Health Science Center.001. including survival and death signaling.The purpose of this study was two-fold: first. Results suggest that salinomycin might be affecting unique biochemical or signaling pathways in a dose and time dependent manner. a natural product and an anticoccidial agent used in the poultry industry. has recently been identified as an anticancer stem cell agent by high-throughput screening. Houston. Direct comparison of the expression patterns revealed a number of apoptosis-related functional gene groupingsuniquely altered at 12 and 24 hours. and 2500 probes at 24 hours at a level of significance of p> 0. A complete assessment of salinomycin-induced apoptosis transcriptome was established using oligonucleotide microarray analysis and confirmed by q RT-PCR. Major canonical pathways include IL-6 signaling. proteomic and morphologic alterations of apoptosis in cells exposed to salinomycin.The high number of probes alteredsuggests the involvement of multiple signaling pathways during salinomycin treatment. p38 MAPK signaling. TX 77030 Salinomycin. Menzies Health Institute Queensland. we look at the growing use of micronutrient supplements during pregnancy and examine the recommendations currently in place to guide the use of these products. this is often not the case. intrauterine growth retardation. fats and protein and the vitamins and essential trace elements needed to support metabolism. providing a healthy start to life for the next generation. Multiple Micronutrient Supplements in Pregnancy: Are They Needed? Are They Harmful? Prof. Keywords: Balanced diet. Lifestyle. many pregnant women are overweight or clinically obese. Griffith University Gold Coast Campus. In this presentation. We also present evidence that broad spectrum micronutrients may have a beneficial effect in pregnancy and lower the incidence of preeclampsia and preterm labour. An adequate and complete diet is important. and this increases their risk of major complications of pregnancy such a preeclampsia. However. A balanced diet and lifestyle is a pre requisite to a healthy pregnancy. QLD Australia . as is the balance between macronutrients such as carbohydrates. preterm birth and gestational diabetes. Healthy pregnancy. It can no longer be assumed that women are consuming an adequate and well balanced diet during pregnancy and the use of micronutrient supplements may potentially have positive effects on a healthy start to life. Finally we focus on the essential trace element Selenium and present a strong case for its importance in maintaining mitochondrial function during oxidative stress. especially in overweight and obese women. millions of women are currently taking these products each year and an opportunity presents itself to systematically determine their beneficial effect. Macronutrients 47 . Southport. which is generated in the placentae of women experiencing these complications of pregnancy. Globally. Tony Perkins School of Medical Science. Interestingly. Systems Toxicology and Health Risk Assessment Group. Dopaminergic neurotransmission 48 . Magic of Curcumin in the Traditional System of Medicine – Unravelling the Molecular Targets to Assess its Protective potential in Arsenic Neurotoxicity Dr. Lucknow – 226 001. India. food preservative and as a household remedy for various diseases in the Indian traditional system of medicine. chemical induced neurotoxicity and neurodegenerative diseases. learning and memory deficits in arsenic exposed rats associated with cholinergic and NMDA receptor alterations were also protected on simultaneous treatment with curcumin. p.o) induced alterations in the expression of NMDA receptors. CSIR – Indian Institute of Toxicology Research. Arsenic induced cholinergic deficits in the brain were also found to be protected.o) for 28 days was found to protect arsenic (20 mg/kg body weight. Further. Khanna Developmental Toxicology Laboratory. The protective potential of curcumin may be attributed to its antioxidant potential and metal chelating property that significantly protected arsenic induced mitochondrial dysfunctions. The high safety profile along with the pharmacological spectrum of curcumin. Nuroprotective potential. 31 Mahatma Gandhi Marg. Vinay K. Curcuma longa. Curcumin. Simultaneous treatment withcurcumin (100 mg/kg body weight.has allured both basic and clinical researchers to assess its protective and therapeutic effects in various pathological conditions including. curcumin was also found to protect arsenic induced alterations in the expression of DA-D2 receptors and signaling proteins known to modulate dopaminergic neurotransmission in rat corpus striatum. apoptosis and ultrastructural damage and provides promising evidence. The focus has been to decipher the molecular pathways involved in the neuroprotective potential of curcumin in arsenic (a metalloid with extensive industrial applications) induced neurotoxicity. its receptor subunits and downstream signalling proteins by modulating PI3K/Akt/GSK3β neuronal survival pathway in rat hippocampus both in vivo and in vitro. an active ingredient isolated from the rhizomes of Curcuma longa (commonly known as turmeric/Haldi) has a long history of use as a spice. Keywords: Curcumin. p. Both subjective and objective criteria were evaluated before and after treatment. Raktamokṣana reduced pain. farmers and laborers. Stabdha and tenderness was however significant (p<0. Keywords: Raktamokṣaṇa.05). University of Colombo. Sri Lanka 2 Institute of Indigenous Medicine. as a case series. The majority of patients were reported to be over weight (52. it is seen that both the treatments are equally effective. Virecana reduced pain.001). Collected data were analyzed using SPSS statistical software. Partial fistulectomy is a recommended procedure of surgery commonly used in perianal fistulas. organs.001). No oral drugs were given. MWSJ Kumari2 1 District Ayurvedic Hospital. 49 . drivers. Galle. Group A was treated with Sirāvedana and Group B was treated with Virecana karma. based on the results. Surgery was planned as incision and drainage and partial fistulectomy followed by Kshara Suthra Therapy from the 3rd day. stepping time and walking time whereas it increased SLRT which are highly significant (p<0. University of Colombo. which are highly significant (p<0. The aim of this study was to reveal the effectiveness of Neelyadi oil local application in recovery of partial fistulectomy followed by Kshara Sutra Therapy. Walking Time (WT) and Stepping Time (ST) whereas it increased femoral stretch test (FST) significantly (p<0. Nānātmaja vāta vyādhi OP 03-02: Effect of Neelyadi oil on local application of partial fistulectomy followed by Kshara Sutra therapy – A case series PGTP Kumara1. The majority of patients were in the 30-39 age group (40%) and female (52. Virecana.5%). or vessels that normally do not connect. Finally. It was diagnosed as Perianal fistula. Virecana reduced spasms and tenderness whereas it increased muscle power and Straight Leg Raising Test (SLRT). Raktamokṣaṇa and Virecana are purification procedures which come under Pañcakarma. Raktamokṣaṇa is therapeutic blood-letting and Virecana is therapeutic purgation. Gṛdhrasi. University of Colombo. Considering the overall effect of Raktamokṣaṇa and Virecana.05).5%) and most were housewives. Sri Lanka A fistula is an abnormal connection that forms between two tissues. SMS Samarakoon2 1 Institute of Indigenous Medicine. The oil was prepared at the hospital pharmacy. sciatica. Institute of Indigenous Medicine. Sri Lanka This study is a comparative clinical study to evaluate the efficacy of Raktamokṣaṇa and Virecana on Gṛdhrasi.OP 03-01: A comparative clinical study on the effect of Virecana and Raktamokṣaṇa in the management of sciatica (Gṛdhrasi) KIE Karandugoda1. Both of these procedures are recommended as treatments for Gṛdhrasi. On admission patients complained of pus discharge from the perianal region for several years or months and pain and swelling close to the perianal region. 40 patients were selected from Gampaha Wickramarachchi Ayurveda Teaching Hospital and randomly assigned into 2 groups A and B. Sri Lanka 2 Department of Deshiyacikitsa. it is concluded that Raktamokṣaṇa and Virecana are equally effective on improving subjective as well as objective parametres of Gṛdhrasi (sciatica). Fistula-in-ano is a chronic abnormal communication between the epithelialized surface of the anal canal and the perianal skin. Decrease in spasms.5%) and obese (27. The effect was evaluated for five patients who attended to the Ano-Rectal Clinic. Hot sit baths were administered before dressing. Neelyadi oil was used for local application after the surgery. Required amounts of oil were applied to the affected area twice daily. It was observed that the wound was completely healed within ten weeks. The main complaints such as pus discharge and pain were 100% reduced. Pharmacodynamics of Neelyadi oil may act to accelerate wound healing and quick relief was observed in these case studies. Further clinical studies should be conducted to prove the effectiveness of Neelyadi oil in a controlled environment, for its scientific validation. Keywords: fistula-in-ano, fistulectomy, peri-anal fistula, Kshara Suthra, Neelyadi oil OP 03-03: A study on the efficacy of Sinhāsyādī decoction on Amlapitta with special reference to gastro intestinal reflex disease KAAL Samarasinghe1, SMS Samarakoon2 , SKMK Herapathdeniya2 1 Ayurvedic Hospital, Aniyakanda, Sri Lanka 2 Institute of Indigenous Medicine, University of Colombo, Sri Lanka Amlapitta which is correlated with gastro intestinal reflex disease (GERD) is a very common disease in general practice. The objective of this study is to evaluate the effect of Sinhāsyādī decoction (SD) on Amlapitta. SD was given to 40 diagnosed patients of Amlapitta in the dose of 100ml twice a day before meals with 10ml bee honey for three weeks. Symptoms were assessed before and after treatment by a grading pattern. Data were collected using a specially prepared research proforma. Data were analyzed by SPSS statistical software using student’s t-test and Wilcoxon Sing Rank test. Most patients were females (70%) and in the 36-40 age group. A burning sensation in the chest and abdomen was the most common symptom (95%). Among the majority of the patients, Amlapitta had gradual onset (95%). Spicy, fatty and heavy meals were the most common aggravating factors, whereas dairy food is the commonest relieving factors. The majority of Amlapitta patients have Vāta-pitta type of prakṛti (57.5%). SD reduced belching, burning sensation, indigestion, fatigue, abdominal pain and flatulence in highly significant manner (p<0.001). It is concluded that SD is effective on Amlapitta. A further scientific study using more appropriate evaluating criteria is needed to validate the finding of this clinical research and to demonstrate the mode of actions of the decoction. Keywords: Sinhāsyādī Decoction, Amlapitta, GERD, Prakṛti OP 03-04: Effect of Māṣabalādī Nasya on Cervical Spondylosis BI Priyadarshani1, SMS Samarakoon2 1 Central Dispensary, Rajarata University, Sri Lanka 2 Institute of Indigenous Medicine, University of Colombo, Sri Lanka Grīvā Sandhigata Vāta is similar to cervical spondylosis based on the nature of the disease and the clinical presentation, which starts in middle age and is associated with ageing. In this study, sixty patients of cervical spondylosis were selected from Ayurveda Central Dispensary at Rajarata University and Provincial Ayurveda Hospital, Anuradhapura. The objective of this study was to evaluate efficacy and effectiveness of Māṣabalādī decoction Nasya for Grīvā Sandhigata Vāta. 50 Selected patients were treated with Māṣabalādī decoction Nasya for a period of 7 days after taking their written informed consent. Data were analyzed by using SPSS statistical software. The majority of patients were in the 31-40 age group and male. Aggravating factors of the disease were excessive work by hand, travelling for long periods, insomnia, writing for long periods of time and computer working. MāṣabalādīNasya reduced neck pain, neck stiffness, headache, numbness in hands and movement difficulties in a statistically highly significant manner (p<0.001). In addition to the pharmacodynamic properties of Mashabaladi Decoction, its Nasya by virtue has a specific effect on the diseases which are above the clavicular region. The drug administrated through the nose as Nasya reaches to the brain and eliminates the morbid doshas responsible for producing the disease. This study concludes that Mashabaladi Nasya is effective on Grīvā Sandhigata Vāta (cervical spondylosis). Keywords: Māṣabalādī decoction, Grīvā Sandhigata Vāta, Nasya, cervical spondylosis 51 52 Track 04 Non-pharmacological interventions -Yoga and meditation for health promotion and medical astrology Abstracts of Keynote Speakers and Oral Presenters 53 respiration and metobolic activity to a standstill. can be used as an adjuvant in disease management and medical rehabilitation. Neural Mechanisms of Meditation-Related Symptom Reduction Dr. Manjunath Nandi Krishnamurthy Director. This presentation would be an attempt to summarize the Neurophysiological correlates of Yoga including studies on meditation. Sara Lazar Associate Researcher in Psychiatry. Present day literature indicates that Yoga can promote positive health. Research. the trends suggest the opposite i. Medical rehabilitation 54 . Positive health. Bengaluru. Harvard University USA In this talk. India Yoga is an ancient Indian tradition and a way of life whose history can be traced back to over 5000 years. Neurophysiological Correlates of Yoga and their Applications Dr. S-Vyasa University.e. While the researchers are interested to see the regions of the brain active during meditation and other practices of Yoga. the process of global inhibition than excitation.anxiety and pain. depression. with an emphasis onhow these changes are related to decreases in stress. Keywords: Yoga. Harvard Medical School. Scientific exploration had focused initially on the exceptional feats great Yoga masters had claimed to achieve such as the ability to bring cardiac activity. Massachusetts General Hospital/ Assistant Professor in Psychology. I will present information regarding the influence ofmeditation practice on brain structure and function.. their applications in health and disease has started hardly three decades back. Systematic approach in documenting the physiological effects of different Yoga practices. was most frequently used as the pediatric massage medium. Thirty-eight (86%) pediatric anesthesia fellowship programs offered one or more complementary and alternative medical therapies for their patients. and has shown its safety in a long history. More high quality evidences for the efficacy or effectiveness as well as to continually observe the safety issues of TCM pediatric massage are still warranted. Darong Wu 2nd Affiliated Hospital of Guangzhou University of Chinese Medicine. There are thousands of categories of massage worldwide because nearly behind each kind of culture exists a kind of traditional massage. among which massage was 35%. TCM pediatric massage has more than a 2000 year-history. 55 . digestive or neurological disorders. massage was 19%. In the United States.5% of the patients were 5 years of age or younger) seeking primary care was 12%. It has been adopted as one of the treatment options for children with commonly accounted diseases. respiratory. among which some are used in children. asthma. Guangzhou. chronic pain. to children with asthma. the overall use of CAM in pediatric patients (67. Although some researches implied the effectiveness of TCM pediatric massage for children with infant diarrhea.g. The application scope of massage for children ranging from helping infant growth or weight gain for preterm newborns. such as coconut oil. TCM Pediatric Massage: A non-pharmacological intervention Option for Children with Respiratory or Digestive Disorders Dr. with some specific acupoints. Among these therapies. Oil. e. cancer. or eating disorder. mostly located on the upper arm. neonatal jaundice. etc. China. The operation procedure of TCM pediatric massage is based on the acupoint selection criteria. which can only be effective for children under 12 years old. TC.g. Body Mass Index 56 . Moreover. and the corresponding ratio was observed together with a concomitant increase in HDL. comparison between naïve and trainer physiological. This data may be useful for installation of Yogaas an important component of Integrative Medicine in Hospitals of the developing world. The present study was conducted in Chandigarh on the eve of 2nd International Yoga Day 2016 where AYUSH Yoga protocol was practised. Yoga practice can enable a homeostatic condition by regulating various regulatory processes with time. Results revealed that after one month of Yoga practice. Department of Neurology. Naïve volunteers. Post Graduate Institute of MedicalEducation and Research. Keywords: Common Yoga Protocol. This study was undertaken to probe whether common AYUSH Yoga protocol can help in balancing the lipid homeostasis in trainer and naive Yoga practitioners. In conclusion the hypocholesterolaemic action of Yoga practice could be considered useful in the management of lipid profile which is considered as major risk factor for various disorders. The Comparative Effect of Common AYUSH Yoga Protocol in Naïve and Trainer Volunteers of Yoga Prof. especially India. iochemical results showed significant changes in both parameters. were estimated and further compared with trainer participants. The physiological and biochemical parameters (e. glucose and lipid profile). Chandigarh. Akshay Anand Neuroscience Research Lab. India. LDL. a statistically significant alteration in levels of serum TG. age and comorbidity. Rajas and Tamas. drug withdrawals. lung volume. Yoga and Naturopathy Education and Research.OP 04-01: Standardization of Yoga practices – An overview KPD Fernando1. Gujarat Ayurved University. standardization OP 04-02: Holistic approach for Prakriti-based medicine: A statistical inference system for analyzing manas prakriti DSK Mendis1.discipline research. Keywords: Yogasana. duration and contraindications . the Tridosha theory forms the basis of Prakriti-based medicine. These are further classified into various subtypes. Yoga is a potent method of health maintenance and disease curing. and economic disparities. However it is difficult to accurately assess one’s Manas 57 . Sri Lanka 2 Department of Swasthavritta. Prakriti. Classically Yoga is a philosophy which contains eight steps towards personal realization and liberation. AA Bhatt2 1 The Ashram Center for Ayurveda Negombo. An integrated approach of modern investigations for physical capacity measurement and classical textual facts for spiritual benefit assessment is needed. place. both physical and psychological. The Ayurvedic concept of Manas Prakrti can be altered through actions/procedures that alter the original or imbibe new qualities in an entity. University of Moratuwa. As the demand increases people tend to practice/teach Yoga without proper knowledge or understanding of the principles. such as adverse drug reactions. Sri Lanka In the 21st century. which in turn leads to harm and disastrous after effects. Prakriti- based medicine and other traditional medicine systems have the potential to offer remedies to challenging health issues. Sri Lanka 4 Gampaha Wickramarchi Ayurvedic Institute. U Samaratunga4 1 Department of Information Technology. sex. Advanced Technological Institute. if practiced in the proper method while also knowing the limitations and benefits. season.to avoid unforeseen health hazards. The objective of this study is to standardize Yoga Asana and Pranayama practices as per personal parameters and method. Jamnagar. General parameters are diet. However. lung capacity. Pranayama. University of Kelaniya. the Manas Prakriti is classified as Satva. This could be a valid subject for fruitful multi. Classical text books of Yoga and Ayurveda were referred to along with modern physiology and anatomy to establish scientific validation and standardization of postures and breathing techniques. For Pranayama. depending on the major contributing factors. Sri Lanka 2 Department of Electrical and Computer Engineering. Whereas the single nucleotide polymorphism (SNP) and epigenetic factors influence drug response and form the basis of personalized medicine. Gulabkunverba Ayurved Mahavidyalaya. Advanced Centre for Yoga Education and Research and Maharishi Patanjali Institute. AS Karunananda3. method. Today practicing Yoga Asana and Pranayama has become a craze or a trend. personalized medicine is all about DNA. India Yoga has been practiced and taught for many generations before it was documented. Personal parameters for standardizing Asana are identified as age. HUW Ratnayake2. Open University of Sri Lanka 3 Faculty of Information Technology. duration of maintenance. diet and Prakriti are identified as important parameters. Thus the need of the hour is to standardize Asana and Pranayama. to name a few. Keywords: Prakriti-based medicine. Manas Prakriti. Sri Lanka 2 Aquinas University College. the quality of life of mothers of children with Cerebral Palsy was significantly different in physical. Initial stage common sense knowledge in Manas Prakriti is converted into a questionnaire. The first group included 30 mothers of children with cerebral palsy who attended the Outpatient Department of Borella Ayurveda Teaching Hospital. Therefore. Manas type in combination (Satva Rajas. T-tests by SPSS were used for statistical analysis. Two sample groups were chosen from a total of 60 mothers who responded to the questionnaire WHO-QoL. University of Colombo. Among the large variety of childhood developmental disabilities. which is constructed on the basis of principal components. A Abeypala2. Falij e Dimagh 58 . The present study was designed to assess and compare the quality of life (QoL) among mothers of children with cerebral palsy and mothers of children with normal development in physical. Presence of a child with Cerebral Palsy in a family generates profound emotional stress and feelings of unhappiness. Keywords: Quality of life. The system was tested with a sample of 88 laymen. Satva Tamas. Classification of the knowledge has been processed through fuzzy logic module. The results of this study revealed that. There were significant differences in the three domains of QoL between mothers of healthy children and mothers of children with cerebral palsy (p<0. Rajas Tamas) can be identified in percentages using the algorithm of defuzzification by principal component analysis. principal component analysis. FMH Haq3 1 Bandaranaike Memorial Ayurvedic Research Institute. Fuzzy logic.Prakriti because of its combinational types. cerebral palsy (CP) is considered to be a major physical disability affecting the functional development of children. appropriate rehabilitation programs for children with Cerebral Palsy and proper psychological support for mothers should be provided to improve their quality of life in different domains.005). Sri Lanka Cerebral palsy (Falij e Dimagh) is a non-progressive neurological disorder of childhood and the prevalence is 2 cases per 1000 live births. social and environmental domains. This shows significant contributions of evaluating Manas Prakriti in combinational types and Manas type in percentage value by achieving a system accuracy of 92%. cerebral palsy. psychological and environmental domains compared to mothers of healthy children. The questionnaire has been constructed with 65 questions and the principal component analyzer detected 23 principal components in the filtering processes. statistical inference system OP 04-03: Quality of life among mothers of children with normal development and mothers of children with Falij-e-Dimagh (cerebral palsy) FRA Raheem1. In this paper we present a statistical inference system in a sub field of the Ayurveda domain of Manas Prakriti to analyze Manas type in combination. The second group consisted of 30 mothers of children with normal development living in Colombo. Sri Lanka 3 Institute of Indigenous Medicine. Removing dependencies among the questions have been modeled using principal component analysis. psychological. age group 30-34 (47%). Keywords: army. University of Sri Jayewardenepura. The result revealed that the majority were male serving officers (83%). rehabilitation. 75% of respondents had not participated in any rehabilitative programmes and reported that existing programmes were unsatisfactory. Encouraging free thinking to abolish the military mindset was amongst the recommendations made along with a holistic approach. married (94%). belonging to the middle class and having higher education qualifications. The study has proven psychosocial rehabilitation is essential for combat military personnel. University of Colombo.OP 04-04: An evaluation of the need of post conflict psycho-social rehabilitation for combat military personnel of the Sri Lanka Army EDK Seneviratne1. The objective of this research was to investigate the need for post-conflict rehabilitation of Army Combat Troops in Sri Lanka. The necessity of social interaction opportunities was requested by 89%. Sri Lanka War has affected every continent in the world. The post-conflict era highlights many issues pertaining to combat military personnel. Sri Lanka 2 Institute of Indigenous Medicine. regarding psychological and physical disorders. post conflict. combat. psychosocial 59 . inclusive of meditation and Yoga. The concept of rehabilitation as a need in society arose greatly after the World Wars. Six key informants were selected for the focus group discussions. MWSJ Kumari2. A self-administered questionnaire was used and 110 participants were randomly selected for the survey study. N Wijewardhana1 1 Department of Sociology. The Data was collected by a survey and focus group discussion. An efficient vocational training system with fitting job opportunities to utilize the experience of combat military personnel as means of advocating reconciliation were recommended for the retired. Financial distress was highlighted as the main socioeconomic problem. disabled and wounded. 65% of military personnel showed higher preference towards combat duties and reported stressful conditions in the post conflict period. 60 . Track 06 Challenges in establishing evidence based practices in traditional and complementary medicine Abstracts of Keynote Speakers and Oral Presenters 61 . The aim of the study is to analyze if the developed “WHO Core and Reference Indicators for monitoring Traditional and Complementary Medicine in South-East Asia” is suitable for policymakers and managers when considering how to monitor performance of their T&CM systems.This situation may challenge the credibility of data collected by using the indicators. The “WHOCore and Reference Indicators for monitoring T&CM in South-East Asia”can be suited for policymakers globally. WHO has prepared a guide with Core and Reference Indicators in order to improve the T&CM monitoring systems. policy kaking 62 . treatment standards or funding of T&CM differs widely. Analyses are based on experiences from the EU FP7 CAMbrella project on regulation of CAM in 39 European countries. It is important that clarifications are developed for each indicator before use. 9037 Tromsø.Policies for regulation. if clarification of definitions and indicators areundertakenbefore use. UiT the Arctic University of Norway. and experiences from one country will hardly be useful for policymakers in another country. Department of Community Medicine. Comparison between countries should beperformed with caution due to different definitions and regulation systems. Faculty of Health Sciences. education. Policymakers and managers lack tools to develop policies and monitor the performance of their Traditional and Complementary Medicine (T&CM) systems. The indicators for monitoring T&CM can be an effective tool for policymakers and managers.The Development of Standard Indicators and Meta Data to Monitor Traditional Medicine System Performance Dr. Keywords: Traditional Medicine. Solveig Wiesener National Research Center in Complementary and Alternative Medicine (NAFKAM). indicators. The document “WHO Core and Reference Indicators for monitoring Traditional and Complementary Medicine in South-East Asia”was reviewed. Norway. acupuncture trials 63 . This presentation will show the bibliometric analysis and methodological quality of clinical trials of Korean Medicine in Korean literatures. We will also present current status of development of EBM clinical practice guidelines on Korea Medicine. Daejeon. We will discuss about the limitation and future direction of traditional medicine including Korean Medicine. Korean Trial Registry and reporting quality of acupuncture trials. Introduction to Evidence based Korean Medicine Dr. Korean Trial Registry. and impact of Korean clinical trials on Cochrane Systematic Reviews. Republic of Korea This study aimed to investigate the current status of evidenced based researches on Korean Medicine. Myeong Soo Lee Korea Institute of Oriental Medicine. bibliometric analysis. Keywords: Korean Medicine. integrated approaches. Lack of understanding and failure to appreciate the importance of fundamental principles of TM and CAM by the researchers in conventional medicine is a major challenge. There are also unique issues in relation to getting ethical approval for such studies. Pilapitiya Director-Gampaha Wickramarachchi Ayurveda Institute University of Kelaniya. D. particularly when they are hospital based. These challenges have contributed to a dearth of such research. professional misunderstandings and conflicts among sectors on sharing resources. Senior Lecturer. Challenges also include regulatory barriers. which intern contributed to mask the true impact of such integrative approaches. Rajarata University of Sri Lanka Traditional medicine (TM) and complementary and Alternative medicine (CAM) practices could be looked upon as useful options for integrating with conventional medical practice. Conducting clinical research in order to generate such evidence on integrated approaches involving TM and CAM face a multitude of challenges. Complementary and Alternative Medicine. The perceived benefits of such integrated approaches are many but needs validation through evidence. clinical research 64 . This could contribute to errors in selecting the study population. Keywords: Traditional Medicine. The unavailability of treatment facilities at which multiple systems can be practiced also pose a challenge for conducting clinical research on integrated approaches. Challenges Faced in Clinical Research Involving Integrated Approaches Dr. Some adapt distorted methodologies to overcome the challenges.Faculty of Medicine and Allied Sciences. designing the TM and CAM intervention. in order to improve some of the preventive and curative health care outcomes in today’s context. The ownership of such integrated approaches can be a challenging dilemma at times discouraging fruitful co-operation among sectors to plan such research. and evaluating outcomes. S. vegetables.You Are What You Eat: Ceylon natural products and lifestyle factors for healthy brain aging leading to Nutrigenomics Prof. Author will discuss neuroprotective role of natural products: Ceylon green and black tea. and how genotype data are linked to the risk of developing a disease and utilized for of early identification of risk factors and timely intervention. life style factors: vegetarian diet in healthy brain aging. Genetic Diagnostic & Research Laboratory and Human Brain Tissue and DNA Repository. Keywords: healthy brain aging. the scientific junction between bioethics. The author focusedtrans disciplinary approach in the effect of Ceylon natural products and life style factors for healthy brain aging that would shed a light on tailor-made nutrition and personalized medicine.Therefore. neurodegenerative disease. lifestyle and genetic factors: gene–diet–disease interaction leading to healthy brain aging and longevity. nutraceuticals 65 . Faculty of Medical Sciences. 2) neuroprotective effect of extract of Ceylon green tea using in vitro hypoxic model with human brain epithelial cells inducing hypoxia. The attempt was to look in to the possible protective role of Sri Lankan diet. nutrigenomics and personalized nutrition in terms of preventing or treating a disease where unique Ceylon natural products and dietary patternlay a stepping stone in developing neuroprotective nutraceuticals.Furthermore. coffee. genetic data should be related to the optimal diet for a certain genotype to reduce the disease risk. (n=2500): one of the largest bio-banks in the South Asian region. Ranil De Silva Principal Investigator.life style factors on healthy brain ageing. Sri Lanka It is important to determine environmental. were studied utilizing:1)the established “Human Brain Tissue (n=76) of aging brains and DNA/Gene Bankof patients and controls with stroke. natural products. University of Sri Jayewardenepura. it shows that there is a remarkable recovery in patients subjected to shiravedhana. weakness of arm and dizziness. WJ Wickramarachchi1. They were treated with Nikādī Thaila Abhyaṅga. Shiravedhana. Various types of treatment are mentioned in Ayurveda. Hypertension and Joint pain also reduced within two weeks. The study was continued for 6 months. MIJ Jayakodi2 1 Sabaragamuva university of sriLanka 2 Ayurvedha health promotion Service Anuradhapura Vericose vein (Shiragranthi) is a disease which become enlarged. varicose vein.as it affects the physical as well as mental and social life of patients. exercises. Thus it can be suggested that the shira vedhana can be an effective adjunct in manage of complicated varicose vein.OP 06-01: A study to identify the efficacy of bloodletting for varicose veins MVSE Dharmapala1. Patients may seek treatment because of the severe pain and unpleasant appearance.Several method of limb bandaging. There is an increasing incidence of this problem in modern society with more desk. None of them were any change. and twisting veins and most commonly affected are in legs and feet. 66 .33%) while rest of 5 showed reduced (16. daily for one month. and Mobile clinic held on Manupa AG division of Anuradhapura. Heaviness. Sarasavi suva piyasa in sabaragamuva university. Sri Lanka 2 Department of Ayurveda. swollen. moderately reduce and no change. All patients were treated with shira vedhana. The results of the treatments were categorized as fully cured reduced. 75% patients pain reduced within one week and completely reduced within 3 months. traditional and allopathic medicine. According to the above study. There is a need to find out the best management of Cervical Spondylosis. Thirty patients were randomly selected from the OPD of Kiriibbanaara ayurvedic hospital. pain with neck movement. Sri Lanka Cervical spondylosis is a chronic degenerative condition of the cervical spine that affects the vertebral bodies and intervertebral disks of the neck as well as the contents of the spinal canal. numbness/tingling sensation in arm. stiffness. The results were assessed on the basis of symptomatic improvement using visual analog scale for pain in the neck.66%) condition. Nikādī Patra pottalī sweda followed by application of Siyambalādī Pattu. In addition.workers. Gampaha Wickramarachchi Ayurveda Institute. injection and surgery are reported to reduce the condition with varying success rates. There are medicinal and surgical treatment approaches to treat this condition however they are often associated with many adverse effects. The present study was conducted on 30 patients with cervical spondylosis selected from OPD/IPD of Gampaha Wickramarachchi Ayurveda Institute.Out of 30. NWGND Gamlath1. A study was carried out to identify the efficacy of bloodletting (Shira vedhana) for varicose vein.bloodletting OP 06-02: A Clinical study to evaluate the efficacy of traditional treatment modality in the management of cervical spondylosis MPN Pramodani1. Keywords : Shiragranthi.Removed blood were 5-15ml per day and continued treatment once a week. AHMY Perera2 1 Department of Cikitsa. headache.3 week. University of Kelaniya. itching and numbness reduced within 1. 25 patients who were treated with siravedhana were fully cured (83. mentioned in Traditional medicine. satisfying the inclusion criteria. . Anemia can also be considered as one significant complicationto arise due to CKD. AB Thaker Department of Kayachikitsa.5 g/dl were selected from Ayurvedic CKD clinic Medawachchiya. Eranda muladi Yapana Vasti OP 06-04: A preliminary study of the effect of Shigruchoorna (Moringa oleiferra) on anaemia of chronic kidney disease patients LSMA Gunawardana. numbness/tingling sensation in arm. KL score. Oswestry disability index and changes in x-ray. Institute of Post Graduate Teaching & Research in Ayurveda. M Kaur. 67 . Sri Lanka Chronic Kidney Disease (CKD) has become a major health problem all over the world.Improvement was observed inpain in the neck. The results showed that the P value of the trial group was < 0. India Approximately 70-80% of the world population has suffered from backache at some point of their life. creed or caste and administered Eranda Muladi Yapana Vasti for 15 days along with recommended classical procedure and diet. S Weerasekara. VAS. Nikādī Thaila Abhyaṅga OP 06-03: Clinical study on management of Kati graha with special reference to lumbar spondylosis with Eranda muladi yapana vasti KPD Fernando. LS is the degenerative osteo- arthrosis of the lumbar spine where Vata at its own Ashaya (Asthi Sandhi Gata) gets provoked. headache.values less than 0. This study concluded that the selected treatment modality is effective in managing cervical spondylosis. Keywords: cervical spondylosis. 60 diagnosed CKD patients with Serum Hb less than 11. T-test was used for statistical analysis. while the control group were prescribed the normal treatment regime only. including in Sri Lanka. Manyā Stambha. and lumbar spondylosis (LS) is responsible for about 10% of these. pain with neck movement with p. Leaves of Moringa oleifera has been mentioned in the management of anemia in literature.05.05. Anemia in CKD patients can be due to less erythropoietin hormone or nutritional deficiencies. Statistically highly significant improvement was seen in the main symptoms and in x-ray changes after the course of Vasti. S Tharmala Bandaranaike Memorial Ayurveda Research Institute. Keywords: Kati Graha. It is recommended that thestudy be repeated in a larger sample and MRI to be used as a better objective assessment tool. Efficacy of the therapy was assessed by reduction in symptoms. Gujarat Ayurved University. WMVS Wijekoon. 20 patients suffering from LS ranging from age 25-65 years were selected irrespective of sex. Eranda Mula is the best drug for pacifying Vata. If not treated it may progress to cauda equine syndrome through spinal canal stenosis or spondylolisthesis. The patient’s serum Hb count was done before starting and after 1 month. The drug Shigruchoornaya was prescribed to the trial group with the normal treatment regime. treatment becomes difficult. Yapana Vasti with milk and other nourishing ingredients gives better results than simple Niruha. The sample was divided into 2 groups: trial and control group. KGP Wijesekara. As it belongs to Madhyama Roga Marga. Jamnagar. At the Institute of Post Graduate Teaching and Research in Ayurvedain Jamnagar. Gampaha Wickramarachchi Ayurveda Institute. adverse effects and recurrence. NWGND Gamlath1. corticosteroid injections. Yuvana Piḍakā can be correlated with Acne vulgaris. WJ Wickramarachchi1. In comparison to the group B. Thirty patients with Acne vulgaris were selected from the OPD of Gampaha Wickramarachchi Ayurveda 68 . NSAIDs as pain relievers and open release surgery. This study has been conducted on 50 patients. University of Kelaniya. especially in youths. Therefore Agni karma treatment can be used as an effective treatment for CTS. numbness and tingling of the hand. Group B patients were treated by the application of Narayana oil for six consecutive weeks. pustule. Sri Lanka 2 Department of Ayurveda. Group A patients were treated by Agni karma with Bindu type by using Panchalauha Shalaka at the site of maximum pain or numbness. Sri Lanka Carpel Tunnel Syndrome (CTS) is a common idiopathic neurological condition caused by compression of the Median nerve at the wrist. Western Medical Practitioners use wrist splints. AHMY Perera2 1 Department of Cikitsa. Keywords: Carpel Tunnel Syndrome. the drug Shrigruchoorna is an effective herbal preparation for increasing Serum Hb levels in Anemia of CKD. This clinical study evaluates the efficacy of Mustādīlepa mentioned in Traditional Medicine. University of Kelaniya. which have their own limitations. Agni karma. Symptoms of CTS include nocturnal pain. group A has shown highly significant results on relieving the signs and symptoms of CTS (p value<0. moringa oleiferra. An effort has been made in this study to assess the efficacy of Agni karma in the management of CTS in comparison with Narayana Oil.Therefore. causes erosions on the face resembling the thorns of a Salmali tree. CKD OP 06-05: The study on efficacy of Agni Karma in the management of Carpel Tunnel Syndrome in comparison with Narayana Oil GMUN Gajasinghe. Narayana oil OP 06-06: Clinical study to evaluate the efficacy of Mustādīlepa in the management of Yuvana Piḍakā (Acne vulgaris) MPN Pramodani1. as described in Ayurveda. AAJ Pushpakumara Department of Shalya Shalakya. Gampaha Wickramarachchi Ayurveda Institute. scars and cysts. nodule. anaemia. papule. CTS is not exactly mentioned in Ayurveda texts. But it can be correlated with the disease of Visvachi which is categorized under Vata Vyadhi. Sri Lanka Yuvana Piḍakā. The patients were followed for three months. macule. Keywords: shigruchoorna.05). Agni karma was done for six weeks with frequency of one cauterization session per week to each patient. Data was evaluated by using SPSS statistical package. between the ages of 30-70 years with confirmed clinical diagnosis of CTS were randomly divided as Group A and Group B. The outcomes of each group were assessed by clinical features with their respective baseline values.Agni karma is recommended for the disorders in the skin and muscles caused by Vata Dosha. Signs and symptoms of Acne vulgaris are comedone. ultrasound waves. satisfying the inclusion criteria. scars and greasiness of the face was observed with p-values less than 0. Signs and symptoms of skin irritation or allergic reaction for the tested drug were not observed during the treatment.05.Mustādīlepa 69 . pain. Keywords: Yuvana Piḍakā.Hospital. macules. tenderness. scars and greasiness of the face as well as fairness in facial complexion by using the von Luschan scale. They were treated withMustādīlepa for thirty minutes daily for one month. Acne vulgaris. pustules. A significant symptomatic relief in eruptions. An increased fairness in facial complexion was also observed after treatment. tenderness. Responses to the treatment were evaluated by using a specially prepared grading scale for eruptions. It is concluded that Mustādīlepa is effective in the management of Yuvana Piḍakā. pain. 70 . Track 07 Strategies for healthy lifestyles through the traditional medical system Abstracts of Keynote Speakers and Oral Presenters 71 . TM related healthy lifestyles and their impacts have been mostly studied from an individual. International Institute for Global Health. The existing studies which showcase inter-sectoral linkages to health and wellbeing such as the “Good Health at Low Cost (GHLC)” have only passing references to TM in those societies. Unnikrishnan Payyapallimana Visiting Senior Research Fellow. TM based practices of health.Showcasing case studies from regions where Ayurveda and other TM knowledge systems and practices have been well integrated into the socio-cultural context. personal health measures and clinical studies. However. alternative or complementary medicine has much to offer in improving health and wellbeing in the contemporary healthcare scenario. Strategies for healthy lifestyles through TM & CM – need for stronger public health research approaches and methodologies Dr. In most societies. University of United Nations. the presentation would argue that focused health system based research approaches with community health indicators are required to analyze good practices to appropriately and innovatively integrate TM in health systems. This is evident in the increasing pluralistic health seeking behavior of the population both in developing countries as well as in industrially more developed countries. Japan Traditional. Keywords: Traditional Medicine. Institute for the Advanced Study of Sustainability. and reflecting on their impact on the overall health of the community. Tokyo. Intergenerational Cultural Learning. There are limited studies on the contribution of TM on health and wellbeing in such societies from the angle of community health or wellbeing indicators. This role of TM in the public health scenario is also progressively being recognized in policy processes and perspectives. Inter-sectoral linkages 72 . or in improving quality of life. lifestyle have been integrated in the communities through intergenerational cultural learning. be it through prevention and management of lifestyle diseases. nutrition. Prakriti of an individual is invariant throughout life and is assessed through examination of a large number of multi-systemic attributes viz. New Delhi. We anticipate that the leads from these studies would enable development of individualized therapeutic interventions envisaged in precision medicine. In a unique CSIR led TRISUTRA Ayurgenomics consortium initiative we have carried out extensive multi-omic studies on Prakriti stratified subjects of diverse ethnic and/or geo. Mathura Road. Precision medicine has been documented and is in practice in Ayurveda for over 5000 years. In the contemporary times. Pitta (metabolic) and Kapha (structural) which governs and maintains homeostasis in the system. India. Homoestatic threshold. Prakriti of an individual is determined by the relative proportions of three physiological entities Tridoshas namely Vata (kinetic). Efforts are focused on tracking and capturing health trajectories of millions of individuals over a period of time and evolve stratified solutions based on Big data analysis of multi- dimensional measurements. Prakriti. primarily for prevention of diseases are needed for reducing the economic burden of diseases. Despite major advancements in genomics.The first step was to probe the ontological links to evolve a molecular language between these contrasting disciplines.Trisutra & Genomics and Molecular Medicine. The life time prevalence of these diseases and increase in average life expectancy further lead to secondary complications due to natural progression of the disease as well as drug side effects. CSIR-Institute of Genomics and Integrative Biology. the advancements in genomics has propelled the field of precision medicine which aims to personalize therapies that are tailored to an individual’s genetic make up as well as their biological state. Individuals of any population can be stratified into seven broad Prakriti types based on proportions of Tridosha wherein the extreme Vata. Pitta and Kapha are most contrasting types. Translational Research and Innovative Science through Ayurgenomics: Insights fromthe TRISUTRA Consortium Dr. Common and complex diseases remain the leading cause of morbidity and mortality across all world populations. Mitali Mukerji CSIR Ayurgenomics. anatomical features. Tridosha. one of the primary challenges that still remains is lack of methods for adequate for risk stratification of individuals for early actionable interventions and individualized management of health and diseases. Disease is a perturbation from an individual’s homoestatic thresholds of Tridoshas and interventions are specifically targeted to restore the baseline states. physiological and psychological attributes. Unit. Coupled to this there is an immense inter-individual variability in susceptibility to diseases as well as response to environment and medications. We hypothesized that integration ofphenotypic stratification of individuals using Prakriti principles and methods with genomicsmight help identify homogeneous groups of individuals and associated molecular markersthat would be informative for predicting different health trajectories. Keywords: Ayurveda. The basic tenets rely on a systems’ understanding of an individual’s constitution type called Prakriti that is important for not only predicting susceptibility and responsiveness to environment but also for management of disease in an individualized manner.climatic regions that represent the genetic spectrum of India. Using an innovative Ayurgenomics approach we have discovered molecular correlates of Prakriti that led toidentification ofgenetic markers that are not only predictive forsusceptibility to diseases but also for differential responsiveness to drug and environment. Ayurgenomics 73 .Innovative and affordable health care solutions. chemokines etc. Department of Nidana Chikitsa. Everyone wishes to live long. Joint diseases. sedentary lifestyle etc have today increased non-communicable diseases like Diabetes Mellitus. The Ayurveda holistic approach towards a healthy life is well elaborated under Sadvritta. Achara-rasayana 74 . Achararasayana and Ayurveda geriatrics almost 3000 years ago. The immune system is the most complex biological system in the human body and is responsible in detecting pathogens by using specific receptors to produce a response by activating immune component cells like cytokines. Swasthavritta.These health issues need a very comprehensive management including a holistic approach.Agni and dhatu in the human body and their integrity. Institute of Indigenous Medicine. happily. Busy lives in congested metropolitans where large populations flock has also become a causative factor for the group of Lifestyle related psychosomatic diseases. Further their subtle and gross relations and interactions with the environment and the Universehas also been discussed. University of Colombo. Ariyawansa Former Professor. Hypertension. Keywords: Non-communicable diseases. Technological advances and excessive urbanization.diseases and the role of well balance of Tridosha. environmental enrichment. Environmental enrichment. Lifestyle Modification and Ayurveda to Enrich the Mental Health for a Healthy Geriatric Life Prof.The Effect of Environment. The Ayurveda classics have comprehensively discussed the concept of a healthy life. Rajagiriya The quest of longevity is eternal. intake of unhealthy diet. Environmental enrichment. Immuno-modulation. Sadvritta. physical exercises etc are considered to be very effective measures to reduce the burden of diseases globally. H.S. lifestyle modification and Ayurveda Rasayanakarma have a positive effect on immune modulatory activity which supports the human body to combat diseases. They modulate and potentiate the immune system of the body. lifestyle modification. Swasthavritta.A. Cardiac diseases etc overwhelmingly prevalent. Although trusted for a variety of health caring and curing potentials. National Institute of Advanced Industrial Science and Technology (AIST). we initiated to develop technologies to obtain Active Ingredients Enriched (AIE) Ashwagandha by manipulating itsenvironmental conditions. and triethylene glycolin the WEX. we have formulated a combination of Wi-N and Wi-A which exhibited potent anti-metastasis activity. family: Solanaceae) is a popular herb used intraditional Indian home remedies. Renu Wadhwa DBT-AIST International Laboratory for Advanced Biomedicine (DAILAB). In view of these findings. withanone (Wi-N) and withaferin-A (Wi-A) in the i-Extract. Based on our studies. Indian Ayurvedic Herb ‘Ashwagandha’ as a Potential Dietary Supplement to Fight Against Aging and Age-related Pathologies Prof. Bioactive. Using multiple experimental and bioinformatics approaches. Keywords: Ashwagandha. Japan Ashwagandha (Withania somnifera. Anticancer activity. suggesting its potency and value as a powerful anticancer drug. and (iii) method of extraction withenriched bioactive components that may serve as cheap. (i) field raised i-Ashwagandha leaves with high proportion of active withanolides as compared to the roots.305 8565. for the first time. and economic anticancer drugespecially when modern medicine is either not available or limited by severe side effects. 75 . Tsukuba. Most recently. We haveinitiated exploration on bioactivities in the leaves of Ashwagandha and found that both alcoholic (i-Extract) and water extracts (WEX) of Ashwagandha leaves possess considerable anticancer activities. Bioactives for anticancer activity were identified as withanolides. but also to telomerase negative (ALT) cancer cells. laboratory evidence and molecular mechanisms of its actionsare only beginning to be resolved. we demonstrated that the two kinds of extracts possess different bioactive constituents and work through independent pathways. we reported thatWi-Ais cytotoxic not only to the telomerase plus. We demonstrate. (ii) hydroponically raised i-Ashwagandha and characterization of its bioactives. New Delhi. Homoeopathy was called in for treating the case. About 100 OPD cases were registered and treated for Mucositis with the indicated Homoeopathic Medicines during the time the patients were receiving Chemo/ Radiotherary. Dysphagia. India Central Council for Research in Homoeopathy (CCRH). One case of B-cell lymphoma of neck treated in the IPD of Delhi State Cancer Institute suffered absolute Dysphagia even after rigorous allopathic treatment. 76 . Government of NCT of Delhi to have collaboration in the field of Cancer treatment. A pilot study was agreed upon to initiate to observe the efficacy of Homoeopathic drugs in treating ‘Mucositis’ after Chemo and Radiotherapy. The detailsof that case is presented here under the following heads. salivation. Ministry of AYUSH. Shaji Kumar Research Officer Central Council for Research in Homeopathic Sciences. swelling. Accordingly the study was initiated by treating the referred patients from the Chemo and Radiotherapy Outpatient Departments (OPD) as well as the patients admitted in the Inpatient Section (IPD). improved sleep and appetite. Homoeopathic Adjuvant Therapy in Oral Cancer Dr. New Delhi had a dialogue with the Technical Administration division of Delhi State Cancer Institute (DSCI). Patients reported marked relief of pain. V SubramanyaIyer and mentioned in Pathaarttaguna Sinthamani. inscriptions. Ingredients are seed of Piper nigrum. free radicals OP 07-02: Prediction of Glycemic Index of coconut jaggery and table sugar through in-vitro analysis HPDT Hewa Pathirana1. Plants are an important source of antioxidants. Sri Lanka A diet with a high Glycemic Index (GI) causes elevated blood glucose levels and is associated with chronic diseases. Glucose was used as the reference food. D M P D Dissanayake2 1 Coconut Processing Research Division. was found by epigraphist K. dated back to the 5th regnal year (1067 A. medicated head bath found in Inscriptions (ARE 248/1923) of Vira Rajendra Chola. antioxidants. It aids digestion and improves lung health. The study determined the proximate composition and sugar profile of table sugar and coconut jaggery. Lunuwila. Siddhas developed a discipline called Kaya Kalpa. The drug consists of above ingredient which is rich in natural antioxidants may have effect on retarding age related degenerative changes. Jaggery is a sweetener made from coconut sap and it is used in traditional medicine to purify blood.towards a healthier Life with special reference to the Siddha tradition P Rohini. Coconut Research Institute.16 %). fruits of Terminalia chebula. Prediction of GI through in-vitro digestion is a promising alternative for clinical GI measurement. loss of different enzyme activities and may cause cancer. Glucose showed the highest TG percentage 77 . rhizomes of Curcuma aromatica.3 %) compared to table sugar. Panchakalpa. Mannar. The objectives of the study are to list out the ingredients of the Panchakalpavati and to explore the efficacy of the drug in ensuring longevity and freedom from illness through review of inscription and siddha literature as well as through scientific studies. Rapidly Available Glucose (RAG). Hydrolysis Index (HI) and GI of table sugar and coconut jaggery were predicted through modified Englyst’s method. pulp of Emblica offinalis and Azadiracta aindica and milk of black cow (Karaampasu) used for both preventive and curative treatments. A carbohydrate containing 0. The highest percentage of RAG (96. Therefore. Free radicals cause many diseases. Lunuwila. NJQ Tharshanodayan District Ayurvedic Hospital. They attack membranes causing oxidation of lipids.OP 07-01: Panchakalpa vati .D). Total Glucose (TG). Keywords: Panchakalpa. which leads to longevity and fountain of youth with complete freedom from illness. Coconut Research Institute.63 %) was observed in the reference food of glucose and lowest (32. Sri Lanka In every country traditional medicines find foundation in religious beliefs and experience. Antioxidants completely stop or delay the process of oxidation. Siddha Treatment aims to provide the ultimate cure to both mind and body systems. HAE Samaranayake1. this study was conducted to evaluate the nutritional status of coconut jaggery compared to table sugar by in-vitro analysis. The WHO is engaged to establish definitive guidelines for methodology of clinical research and the appraisal of effectiveness of traditional medicine. ash (2. Coconut jaggery showed a significantly higher percentage of fiber (0.38 %) was observed in coconut jaggery. Sri Lanka 2 Soils and Plant Nutrition Division. LLWC Yalegama1.5g sample was put into polypropylene tubes with small glass balls and subjected to enzymatic digestion at 37° C in a shaking water bath.7 %) and sucrose (96. 60. Replacement of fat by dairy proteins can be used to formulate very low GI milk powder. Sri Lanka 2 Department of Biochemistry. 30. Mind and Body medicine is related to the fact that our general health depends on 78 . Average Glycemic index was determined to be 12±5.05%) whereas the lowest was in coconut jiggery. table sugar OP 07-03: Effect of reduced fat and increased protein content in milk powder on Glyceamic responses in local dairy cows S Manokaran1. Faculty of Medicine and Allied Sciences. This study was designed to determine the Glycemic responses in a protein enriched (36. The average peak reduction of blood glucose response curves was 24.31 % of predicted glycemic index (PGI) could be observed indicating that it is lower than the PGI of table sugar (73.5 . after reduction of fat and addition of extra whey protein.05) in GI with the formulated milk powder was observed. milk OP 07-04: Preventive ophthalmology. I Wickramasinghe1.79%). University of Sri Jayewardenepura. Keywords: coconut jaggery. I Wijesekara1. Lower HI of 32 % was observed in coconut jaggery in which 57. contributing to 32. the eyes are considered to be the prime.(54.23. KKDS Ranaweera1.38%. Results showed a reduction (p<0. With these results. Glycemic Index. 90. Healthy volunteers (n=10) consisting of five males and five females between 20-25 years with body mass index (BMI) of 18. GI value was determined as an average of all 10 subjects.an Ayurvedic perspective N Bavalatti All India Institute of Ayurveda Gautampuri. It has been rightly told that 'Sarvēndriyānāmnayanampradhānam' means that amongst all sense organs. MA Jayasinghe1.5%) milk powder formulation made using milk collected from local farmers. Rajarata University of Sri Lanka Milk is considered a low glycemic food. SPAS Senadheera2 1 Department of Food Science and Technology. 120 minute intervals after ingestion of the standard (Glucose) and formulated milk powder containing 50g of digestible carbohydrates. The study concludes that the impact of proteins in milk to reduce blood glucose responses exceeds that of fat. coconut jaggery can be recommended as healthier than table sugar. Steps include the Dinahary (lifestyle during day).4%). Blood glucose concentration was measured by finger pricked capillary blood samples at fasting. Glycemic index (GI) is a measure of the effect of carbohydrates on blood glucose responses. in-vitro. low-fat.1%. diets and routines and even care during the seasonal changes. The GI value of typical milk powder is 35±1 (David et al.5 kgm-2 were selected. Rathricharya (lifestyle during early evening and night). Saritavihar. India Preventative ophthalmology is an extremely important concept which needs to be given adequate importance to avoid early eye conditions from progressing into Disease. Keywords: high-protein. New Delhi. About 90% of learning will be gained by visual senses. Acharya explains the preservation of eye health (Nethraraksha). 1981). glycemic index. Milk was collected from local farmers and spray dried. low fat (4. 15. Faculty of Applied Sciences. Padabhyanga (massage to feet). the work environment and the level of exposure to airborne toxins.all the individual parts working together effectively. Chakshushyaraasayan. Kriyakalpa 79 . etc. diet. A balanced diet. and intake Chakshushyaraasayana (specific affinity for rejuvenation of ophthalmic tissue) as a routine will help to prevent the manifestation of eye disorders and will preserve normal vision. Eyesight is also dependent upon our total wellbeing including our genetic structure. Pratimarshanasya (nasal administration of medicine) etc. Keywords: healthy eyes. regular eye exercises along with following specific procedures explained in Ayurvedic ophthalmology like Anjana (Collerium). 80 . Track 08 Quality assurance and standardization of products of traditional medicine Abstracts of Keynote Speakers and Oral Presenters 81 . Cosmeceuticals. Applications of nanotechnology such as nanovesicles can solve these problems. nanotechnology 82 . Thailand / Division of Cosmetic Technology. Several recipes and plants from the “Manosroi III” database have been developed as high commercial potential products using nanotechnology. Thailand Thailand has the advantages of biodiversity and traditional medicines from folklore wisdoms to be developed as efficient and safe topical pharmaceuticals and cosmeceuticals. Chiang Mai. have skin irritation and low skin permeation. Faculty of Engineering and Technology. North-Chiang Mai University. Niosomes are nanovesicles which are bilayer vesicles composed of non-ionic surfactants which can entrap both water and oil soluble substances. Aranya Manosroi Manose Health and Beauty Research Center Chiang Mai. This presentation has demonstrated not only the benefits of the “Manosroi III” database. and MMP-2 inhibition on human skin fibroblasts. Cosmeceuticals containing Terminalia chebula gall extracts encapsulated in nanovesicles showed anti-aging activity with high DPPH radical scavenging. Bioactive compounds existing in natural products are chemical unstable. Herbal Balls. Terminalia chebula gall. but also the applications of nanotechnology for Thai folklore wisdoms to develop as effective and safe products. The targeted recipes and plants can be searched from the Thai Medicinal Plants and Recipes “Manosroi III” database using keywords. The topical pharmaceutical spray containing extracts of mixed Thai traditional herbal ball herbs encapsulated in niosomes indicated high anti-inflammation activity of ear edema inhibition in rats with no skin irritation in human volunteers. Keywords : Topical Pharmaceuticals. Topical Pharmaceutical and Cosmeceutical Product Development from Thai Traditional Medicines “Manosroi III” Database and Nanotechnology Prof. This may be due to lack of knowledge regarding the prognosis of the diseases and lack of facilities available to manage the NCD patients for prolonged periods. University of Colombo. These medical systems are utilizing natural resources in dealing with the NCDs and other health related problems. I strongly feel that this symposium would definitely trigger all of us to work towards this worthy task to fight against NCDs. have answers to address the preventive and curative aspects of the NCDs with least or no side effects. heredity and environmental factors. But can also occur due to genetic abnormalities. M. Rajagiriya Sri Lanka Non-communicable diseases (NCDs) are a major threat to the health sector. NCDs are also known as chronic diseases owing to their prolonged duration and generally slow progression. It is my ardent hope that there should be an inter-disciplinary approach to handle the NCDs by treating the acute phase of the NCDs with synthetic/ modern drugs and then to switch on to traditional treatment modalities as follow up therapy in order to prevent the side effects of the synthetic drugs and thereby to save the patients with NCDs from catastrophic drug induced diseases. NCDs mostly occur as a result of four particular behaviors of the human being namely. Although the allopathic medical fraternity has the facility to diagnose these conditions there are no promising treatment methods available to eradicate NCDs. least side effects 83 . tobacco use. who is also considered as father of medicine by the west says “let food be your medicine and medicine be your food”. Nazeem Senior Lecturer in Unani Medicine. NCDs are the major cause for premature deaths especially in low and middle income countries. This unfortunate situation puts the NCD patients in misery. physical inactivity. Therefore it is high time to scrutinize and then introduce traditional medical treatment modalities to fight against the NCDs. The famous architect of Unani medicine. Hippocrates. Further the medicines prescribed to control NCDs produce serious side effects targeting the functions of the vital organs of the body. Keywords: NCD. This version exhibits the fact that therapeutic intervention should not be the first line management in any disease and also matches with the present days’ nutraceutical concept. NCDs can be Controlled with Traditional Medical treatment Modalities with Least Side Effects Dr. H. unhealthy diet and harmful consumption of alcohol. Institute of indigenous Medicine. M. which are nature friendly. I congratulate all of you and hope this symposium would concentrate in finding answers to NCDs. patient friendly and also cost effective. The traditional medical systems. Traditional Medical Treatment. I presume that we all have failed to educate the public in an organized manner regarding the modifiable risk factors of NCDs which is cost effective and a drug free method. Keywords: Lead Compounds. Faculty of Engineering and Technology.000 recipes for several diseases. Improved Sexual Dysfunction. a lead compound isolated from N040 showed high anti-cancer activity. Chiang Mai. Traditional Medicines. MP-4 indicated the highest estrogenic activity of 1. Clinical trial of N040 capsules in cervical cancer patients exhibited an improved quality of life. EDR2-N03 demonstrated the highest PDE inhibition activity and the improved reproductive behaviors of the sexual dysfunction-induced male mice in comparing to sildenafil. Recipe no. Recipe no. Thailand A large number of recipes from all regions of Thailand including Lanna has been collected. a standard PDE inhibitor. N040 showed the highest anti-proliferative activity on HeLa cell line of 31. selected. Brazilin. Research and Development of Lead Compounds for Modern and Traditional Medicines/Food Supplements from the “MANOSROI III” Recipe Database Prof. Division of Cosmetic Technology. 84 . The application of traditional medicines from “Manosroi III” database will not only be a short cutting strategy. Anti-cancer.09 folds of cisplatin. Thailand.03 folds of β- estradiol. It also indicated anti- tumor activity in HeLa xeno graft nude mice with the maximum inhibition percentage of 57. Jiradej Manosroi Manose Health and Beauty Research Center Chiang Mai. North- Chiang Mai University. MANOSROI III Recipe Database. translated from the Lanna (the dead language) to the modern understandable Thai and recorded in the Thai medicinal plant recipe “MANOSROI III” database. Recipe no. but also an efficient tool for the development of novel modern drugs.23 % and high safety insub-chronic toxicity study in rats. This database contains 90. Market sample. purgative and an emmenogogue. V. Faculty of Science. University of Jaffana.Unit of Siddha Section. This aloe is one of the main ingredients in external applications such as poultice. Sathiyaseelan Head. constipation. of Chemistry. Screening of Important Phyto-Chemical Compounds: Comparative Study of Prepared Black Aloe and a Marketable sample of Black Aloe Dr. Jaundice and skin disorders. University of Jaffna for the Phytochemical Screening. tonic. Keywords: Phyto-Chemical. Sri Lanka Secondary metabolites are the classes of compounds which are known to show curative activity in several ailments in humans and therefore could explain the use of medicinal plants for the treatment of several ailments. Black Aloe is a favourable remedy in rheumatic affections. This shows that the prepared sample has more compounds which act as a potent drug than the market sample. The Black Aloe was prepared by a Pharmaceutical process in the pharmacy of the Unit of Siddha Medicine. menstrual disorders such as amenorrhoea. This prepared sample and the market sample was sent to the Dept. The Black Aloe of Aloe vera selected for this study belongs to the family of Liliceae.Based on the results obtained the analysis showed that tannins present with saponinsAnthracenosids and Anthocyanosids are present in the prepared sample while tannins present with only Anthracenosides were in the market sample. Before the study the plant was collected from the Delft Island and authenticated by the taxonomist. atonic dyspepsia. Prepared sample. It is a stomachic. Black Aloe 85 .This phytochemical analysis showed some compounds are not present in the market sample. safety and efficacy of medicinal plants and their products has prevented such lucrative ventures. Most of the herbal raw materials for western industries are obtained through the suppliers in Asian countries such as India and China. 86 . at least partially due to the skyrocketing healthcare cost. the use of herbal and medicinal plant products. the current global trend in medicinal plant research and the importance of establishing botanical. often as “dietary supplements”. There is a huge potential to cultivate medicinal plants to supply raw material for these industries and also to develop various value added products for the export market. Keywords: Medicinal Plant Products. Even the quality and safety of products available in local markets are questionable. Medicinal Plant Research & Industry: Opportunities and Challenges Prof. However. the market for herbal products reached $6 billion last year. Aruna Weerasooriya Professor and Research Scientist. the lack of comprehensive research on quality. In the US alone. “Dietary Supplements”.Therefore. has become mainstream in the western countries. the export market not well established for the products due to the lack of comprehensive scientific evaluations. chemical and genomic standards of medicinal plants in Sri Lanka will be discussed as a basic requirement for the development of quality marketable herbal products. Although Sri Lanka has higher diversity of medicinal plants whose effectiveness has been proven across many generations. Texas A&M University System. USA Over the past two decades. tannins.40 ± 0. HBK Ajith Kumara2 1 Sārārtha Ayurvedic Medical Center.In conclusion. total flavonoid contents of decoctions prepared according to method 1.20 ± 0. Tinosporacordifolia (Amruta). and sitting in a squatting position. Sri Lanka 2 Research and Development Complex. Nawinna. Results revealed that phytochemicals such as phenols. Barleriaprionitis (Sahachara).45± 0.64± 0. climbing upstairs. 32 patients were selected who were suffering from Sandhigata vata in Knee joints at OPD of Bandaranaike Memorial Ayurvedic Research Institute and 87 . Boerhavia difffusa. Industrial Technological Institute. All these phytochemicals are reported to possess various pharmacological actions and anti-oxidant properties. Azadirachta indica.56. Tragiainvolcrata (Dushparsha). Keywords: Madhyama Rasnadi decoction. Sri Lanka 2 Bandaranaike Memorial Ayurvedic Research Institute. RHSK De Silva1. Alpiniacalcarata (Rasna). The selected pastes include. Tamarindus indica leaf extraction and Terminalia chebula powder. It can be suggested that the method described in Sharangadhara Samhita is the most effective method of preparation. Traditional method (method 1) and methods described in Sharangadhara Samhita (method 2) and Bhaishajya Ratnavali (method 3). Patients experience difficulty in walking. indicated for Ama and Vata Janya diseases. more phytochemicals are concentrated in the decoction made with method 2. The ingredients of the decoction are. Vigna unguiculata. The literature provides 3 different preparation methods of this decoction viz. 86. HGSG Wijesiriwardhana1 1 Department of Desheeya Chikitsa. University of Colombo. The first step was studying the traditional books to identify a proper medicine. alkaloids and terpenoids were present in all 3 types of decoctions. Cyperusrotundus (Ghana). flavonoids. Total polyphenolic contents of decoctions prepared according to method 1.30± 0. phytochemicals. Adhatodavasica (Vasa).65. In addition. Cedrusdeodara (Devadaru). LDAM Arawwawala2. The drug was prepared in ‘Lepa’ formation and packed on 25g Aluminium foil packets. This was carried out by (a) screening of phytochemicals and (b) quantification of polyphenols and flavonoids. Terminalia chebula (Abhaya). Kaempferiagalanga (Shathi) and Zingiberofficinale (Shunthi). Ricinuscommunis (Eranda). 2 and 3 were 69. Asparagus racemosus (Shatavari). Sri Lanka Sandhigata vāta with similar relations to Osteoarthritis is a painful joint disorder associated mostly around knee joints with cracking and swelling.80. The current investigation analyzed the phytochemical profiles of the aforementioned 3 preparation techniques. 2 and 3 were 121. Sri Lanka Madhyama Rasnadi is a herbal decoction.50 mg quercetin acid equivalents/g extract respectively. 178. Aconitum heterophyllum (Ativisha).68 ± 0. Gallic acid and quercetin were taken as standards to express the results of polyphenolic and flavonoid contents respectively. LDC Sandun1.60.OP 08-01: Qualitative and quantitative phytochemical analysis of three different preparation techniques of Madhyama Rasnadi decoction RLDS Ranasinghe1. The main objective of this research was to assess the efficacy of Lepa (paste) locally in the management of Sandhigata vāta and to study the clinical improvement. anti-oxidant properties OP 08-02: Preparation of ‘lepa’ which is effective for Sandhigata Vāta MPWL Samarawickrama1. saponins. 129. 52. Institute of Indigenous Medicine.25 mg gallic acid equivalents/g extract respectively. Shotha. S Kaluthotage1. Shodhana Cannabis (Purification process) was done by three different methods. 56. Hence Lepa is very effective for Sandhigata vāta. in cow milk dolapaka and fried with ghee (Sample 4). KCS Jayaweera2. BADH Kularathne1. Symptoms called Shoola. Sri Lanka 3 Institute of Indigenous Medicine . It is used for medicinal purposes in treatment of nausea. W Deshapriya1. The three processes of purification and detoxification facilitated increased Tetrahydrocannabinoic acid. The study shows purification methods which play an important role in increasing anti-psychoactive agents. and can be attributed to the treatment.88%. beneficial compounds 88 .13%. Though it is categorized as a dangerous drug. University of Kelaniya. it is successfully applied in a considerable number of diseases in western and eastern medical methods. Sandhi Graha. neuro-protective compounds and removing non. Sri Lanka Cannabis is common used in indigenous medicine in Sri Lanka.beneficial chemical compounds of cannabis. Cannabidiol and significantly decreased harmful Cannabinol and Tetrahydrocannabinol. SN Priyangika3.25% and 62. They were observed weekly for a month and recorded their progress according to the questionnaire designed for this research and analysed via SPSS software. The four samples were developed in High Performance Liquid Chromatography (HPLC). W.Gunapala Gampaha1. Sri Lanka 2 Wickramaarachchi Ayurveda Institute. Cannabis is incorporated in Ayurvedic drugs after the process of purification (Shodhana) and detoxification (Marana). Sandhivāta lepa OP 08-03: A comparative study of the chemical constitution of raw cannabis and purified cannabis LHS Umayangani1. Keywords: Sandhigata vāta. University of Colombo. Sandhi Sputana.50%. but there is no meteorological data for post-process changes. The objective of the study was to differentiate between the chemical constitution of raw cannabis and purified cannabis. Ayurveda describes the purification process for poisonous plants. It is a common drug in treating gastro intestinal problems which arise as side effects of radiotherapy and chemotherapy in cancer patients. Keywords: cannabis. NRT Nalawaththa3. vomiting and to stimulate hunger during chemotherapy treatment. such as soaked in hot water and fried with ghee (Sample 2). Sparsha Asahyathwa Vedana and Akunchana Prasarana Janya Vedana have reduced respectively in 28.the drug was given to apply locally around the knees. 43.05% (95% confidence interval). detoxification. A. thriphaladolopaka and fried with ghee (Sample 3). KS Priyadarshini3 1 Bandaranaike Memorial Ayurvedic Research Institute. 100%. HPLC. Therefore it was confirmed that the average loss of each symptom is not due to chance variation. purification. The raw cannabis was Sample 1. it is called Sandhivāta lepa. The significance values for change in all of the six symptoms were less than 0.33%. 21.Senewirathna1. Klebsiella pneumonia(10mm. University of Sri Jayewardenepura. The formulation was identified as a oil in water type emulsion by Crystal violet staining. phase separation. odour. Pseudomonas aeruginosa (ATCC 25853). Acinetobacter baumannii (10mm. clinical isolates Klebsiella pneumonia and Acinetobacter baumannii. PRL Dabare1. 2. Sri Lanka 3 Department of Nidana Chikitsa. HGSP Hewageegana3. emulsion type and antimicrobial activity was recorded for up to 6 months. The 2x concentrated aqueous cream had better antimicrobial activity of the two formulations.12mm). University of Colombo. stability. LDAM Arawwawala4. TDCP Gunasekara2 1 Department of Allied Health Sciences. Sri Lanka 2 Department of Microbiology. phytochemical analysis. This study aimed to develop and evaluate the antimicrobial properties of an aqueous cream prepared from aqueous herbal extract of Garcinia zeylanica. Zones of inhibition were observed against Staphylococcus aureus(12mm. antimicrobial activity during the time period 0. Sri Lanka 5 General Hospital. Institute of Indigenous Medicine. MAL Senevirathna1. This novel formulation is a potential candidate for development of a commercial herbal antiseptic. Formulated aqueous cream had good physical properties. Escherichia coli (11mm. Dried pericarps of Garcinia zeylanica were used to prepare an aqueous extract according to standard ayurvedic practice. Keywords: Garcinia zeylanica. pH. Colour. A Siriwardhene1. Aqueous creams containing 1x and 2x concentrations of GZ extracts were formulated in emulsifying ointment BP with and without preservatives according to the British Pharmacopoeia 2013. BP Kudavidanage5. Escherichia coli (ATCC 25922).16mm) after 6 months at 250 C for 1x and 2x concentrated formulations respectively. Garcinia zeylanica aqueous cream had good stability and antimicrobial activity. The dried pericarp has many uses of which it is a component used as a wound wash in Ayurvedic practice. Sri Lanka Garcinia zeylanica (GZ) is an endemic plant in Sri Lanka with reported antimicrobial activity. Sri Lanka 4 Industrial Technology Institute. Kegalle.OP 08-04: Development and in-vitro evaluation of a Garcinia zeylanica aqueous cream as a potential antiseptic UK Anuradha1.12mm) and Pseudomonas aeruginosa(12mm. MMK Peiris2. The antibacterial activity of aqueous cream were determined by well diffusion method against Staphylococcus aureus (ATCC 25923).13mm). 1. University of Sri Jayewardenepura. aqueous cream. antibacterial activity 89 .17mm). 3 & 6 months. 90 . Track 09 Pharmacological studies of traditional medical ingredients Abstracts of Keynote Speakers and Oral Presenters 91 . Codonopsis lanceolata. Different type of Korean ginseng wereexported to Japan. of Biotech. Rubusco reannus (1.000 sq km and consists of 64% forest. lavender et al) was only 14 ha. In Korea about 480 wild plants are edible. Total amount of ginseng exports was 5. Cultivated area for western herbs (rosemary. These 4 cultivated crops accounted for 70% of the total wild vegetable production area. Main crops were Shizandra chinenesis (2. but only 34 plants were cultivated for food and 53 for medicine in 2016. Production and Marketing of Wild Vegetables. Codonopsis lanceolata (2. Medicinal Plants and Ginseng in Korea Prof. Cultivated area for Ginseng was 14. Cultivated area for medicinal plants was 12. Korea imported about 97million dollars of medicinal herbs. mainly 76% from China. W. Since ancient times. China. Keywords: Pteridium aquilium. Wanjugun. Park Div. Korea The total area of Korea is 100. Cultivated area for wild vegetables totaled 12. and Aster scaber (751ha).660 ha). Total value of imported wild vegetables was about 45 million dollars in 2016. Isemyen. Platycodon glandiflorum (1255ha).828t and 133 million dollars. Coll. Koreans have collected many wild herbs not only for culinary purposes but also for medicine. Platycodon glandiflorum 92 . USA.679ha and produced 20. Dried Pteridium aquilium. Codonopsis laceolata (2. fresh root of Platycodonglandiflorum and fresh roots of Codonopsis lanceolata were mainly imported items. Hong Kong.687ha). 20% arable land and 17% other.140 ha in 2016.386 t in 2016.140ha in 2016: Pteridium aquilium (3. K. Vietnam and Taiwan.229ha) and Zinziber officinale (1046ha). of Biosci. Chenbuk.540ha).454 ha). As an example. mal practice and carelessness. Dicot.which is helpful in identification of certain drugs. good quality Amukkara ala can be guessed by a short fracture which indictes less fibre content. Kokumpotu shows orange colour in its outer bark. Keywords: adulteration. high demand. Harankaha can be easily differentiated from wal-inguru by the presence of a purple line on the mid-rib of the leaf. like “Belimul” since it is a root drug. morphological charactrristic. microscopical. exhausted cloves will not sink in water but genuine one sinks due to higher specific gravity compared to water. microscopical. organoleptic. Identification of Adulteration in Herbal Raw Materials Dr. similar appearance similar to original drug. Sri Lanka Adulteration is a practice of substituting an original crude drug with similar looking substances having inferior chemical and therapeutic properties. Stem has a pith but not a root. use of same name for different species. Malabe. physico-chemical. coconut oil are being adulterated in the market and those can be identified with validated Pharmacognostical methods and this is one of the ways to minimize such adulteration in the herbal drug industry if implemented by herbal industries. Industrial Technology Institute. sesame. General methods to detect adulteration are by morphological. Fixed oils such as caster. T. histochemical and by chemical methods as per standard Pharmacognostical protocoals. Microscopically. cow’s ghee.D. pharmacognostical protocols 93 . Basic knowledge on Botany will be useful in identification of crude drugs.K. Reasons for adulteration are. good quality Caster oil is thick and is felt by touch. organoleptic. less supply.high price. Binkohoma and heen-binkohomba can be differentiated by the absence of Calcium carbonate cystolith in the leaf of binkohomba. Wijayasiriwardena Principal Research Scientist.M. Bee honey can be identified by its peculiar taste & smell and presence of pollen grains. it should not have pith at the centre area of the root.C. Safety Studies on Mineral and Herbomineral Drugs Used in Ayurveda – A Point of View Dr. B. Ravishankar SDM Centre for Research in Ayurveda and Allied Sciences, Udupi, India Mineral and metal based formulations form an important component of Ayurvedic therapeutics. They have a long history of use as effective and safe remedies. However, in the past few years concerns are expressed at international level about the safety of their use in therapeutics. Discussion of important aspects of this issue is the presentation focus. The Issues considered are; equating the toxic potential of inorganic and other forms of minerals and metals with ayurvedic ‘Rasaushadhies’; categorization of toxicity reports on the basis of appropriate and inappropriate use and use by authorized or unauthorized persons; and to evolve appropriate safety evaluation protocol. Review of available literature shows that speciation or characterization profile of the metallopharmaceuticals used in Ayurveda is not available. Hence, equating toxic effects of heavy metals with ‘Rasaushadies’ is not appropriate. The majority of the studies carried out on classical products do not reveal any serious toxic potential when used in an appropriate manner at proper dose levels. Many factors like-source materials used, the manner of processing, purification and preparation process employed, use of adjuvants; the clinical condition of the recipient, bio-availability of the products and their interaction with cellular ligands have influence on the safety profile. Examples will be provided during the presentation. Safety evaluation protocol adopting the latest advances in the field of toxicology like toxicogenomics, computational toxicology, in vitro methods using cells, cell lines, or cellular components, preferably of human origin and using non- vertebrates needs to be developed. Data generated thus can form a basis for detailed studies. Keywords: Ayurvedic therapeutics, Rasaushadhies, Metallopharmaceuticals, toxicogenomics 94 Australian Native Plants: Novel Therapeutics for Global Infections and Diseases Dr. Trudi Collet Senior Lecturer in Pharmacy, Faculty of Health, School – Clinical Sciences, Queensland University of Technology, Australia In 2016, two health threats confronted the world: the alarming increase in hospital-acquired infections due to increasingly pathogenic antibiotic resistant bacteria, and the emergence of the Zika virus.Antibiotics are central to the global healthcare system, reducing the burden of common infectious diseases and underpinning positive outcomes from medical interventions. The recent spread of pathogens such as Methicillin-Resistant Staphylococcus aureus (MRSA), vancomycin-resistant enterococci (VRE) and colistin-resistant Escherichia coli has depleted the supply of efficacious antibiotics. Globally, the widespread emergence of resistant bacteria already claims 700,000 lives per year. Further, it is predicted that by 2050, 10 million people will be killed annually i.e. one every three seconds. Thus, the emergence and spread of antibiotic resistant pathogens among human and animal populations worldwide has shifted attention to the need for more broad-spectrum antibiotics. To that end, using the well diffusion assay, we evaluated the bactericidal potential of extracts obtained from an Australian native plant (denoted species 8472). Of the 16 bacteria screened, only Klebsiella pneumoniae was found to be impervious to the extract. Overall, species 8472 was shown to be highly potent against both Gram-positive (MRSA, VREs, Bacillus cereus, Streptococcus pyogenes) and Gram-negative (E.coli, multi-drug resistant Acinetobacter baumannii, Proteus spp.) bacteria. These findings will have a significant global impact given the dearth of antibiotics currently available that have the ability to effectively clear an infection caused by antibiotic resistant bacteria. Keywords: antibiotics, antibiotic resistant pathogens, broad-spectrum antibiotics, Australian native plants 95 Application of Metabonomic Analytical Techniques in Traditional Medicine Research Prof. P. A. Paranagama Faculty of Science, University of Kelaniya, Sri Lanka Currently a wide range of metabonomic analytical techniques are widely used in Traditional Medicine (TM) research and since the demand of the traditional medicine (TM) is increasing tremendously, assurance of safety, quality and efficacy of TM are becoming a crucial issue. Hence there is an urgent need to develop a systematic and optimized method using reliable analytical techniques for the standardization of TM raw materials. TM are composed of many chemical constituents and it is very important to develop consistent chromatographic fingerprints that signify pharmacologically active and chemically characteristic components in TM. The advancements in modern analytical methods and the development of application have made it possible to solve many of the problems related to analysis of TM. The valuable modern analytical techniques used for TM analysis include HPTLC, GC, GC-MS, MS, HPLC and LC-MS. Phytochemical profiles of TM have to be generated and the need of high-technological oriented applications has given rise to investigate and serve as rapid unambiguous tools in TM research. Further development of metabonomic analytical techniques will promote TM research globally and this will be beneficial to the development of TM, assist the formulation of TM safety, and establishing the international standards indicators. Keywords: TM, HPTLC, GC, GC-MS, LC-MS 96 Hippocampal Neuron Culture for Analysis of Neurotrophic Factor-Mimetic Activity: Application to Traditional Medicinal Plants and Marine Algae Prof. Il Soo Moon Department of Anatomy, Dongguk University Graduate School of Medicine, Gyeongju 38066, Republic of Korea Neurotrophic factors (NTFs) promote the initial growth and development of neurons and in the mature nervous system, they promote neuronal survival, induce synaptic plasticity, and modulate the formation of long-term memories. Therefore, most neurological disorders are attributed to lack of NTFs. Medicinal plants have served as traditional folk medicine world- wide and also as a superb reservoir of preventative and therapeutic multitarget-directed natural ligands for brain disorders. So far, a pheochromocytoma PC12 cell line that is derived from the rat adrenal medulla have been used in the screening assay for NTF-mimetics. PC12 cells, however, are not derived from CNS but a blastoma derived from the neural crest, an origin of PNS, and they do not differentiate into neurons which are morphologically and functionally close to brain ones. In contrast, primary hippocampal neurons mature in vitro into those that are very similar to brain ones, and the morphological development is very stereotypical from initial neuritogenesis, axonal and dendritic outgrowth, to later dendritic maturation and synaptogenesis to produce functional synapses. My laboratory took advantage of this culture for screening many inland medicinal plants and marine algae. In this talk, I will share and discuss some of our recent data. Keywords: Brain Disease, Neuritogenesis, Neurotrophic Factor, Primary hippocampal neuron, PC12 97 it is used with a blend of Gandhaka (Sulphur) in which the basic preparation is called Kajjali. Rasaushadhis are considered superior than herbal products. Kajjali 98 . Pottali and Kupipakwa are the main preparations mentioned in Rasa Shastra. quick effectiveness. Karaliya preparations are prepared by using motor and pestle whereas Kupipakwa is prepared in side of a glass bottle. mercury is not used as it is. K. Herapathdeniya Senior Lecturer. there are five types of Mercury cited. mineral and animal products are being used for the treatment of different ailments. Gandhaka. As per Rasashastra. Due to their small dose. apart from Shodhana. In Ayurveda as well as traditional medicine herbal. mercury must be subjected to a procedure called Shodhana. According to Rasarathnasamucchaya. It also mentions several acceptable as well as unacceptable properties of Mercury. M. K. Karaliya. S. Rasaushadhi. Parpati is a wafer like preparation and Pottali is a unique preparation used in patients who are in relentlessly ill condition. Institute of Indigenous Medicine. Parpati. These mineral preparations are called as Rasaushadhies which is the core of Rasashastra and Mercury is the chief material. Kajjali is used as a fundamental ingredient in various formulations as an adjuvant and also itself as a medicine. Mercury must be subjected to VisheshaShodhana and ParadaSamskara. Instead. Usage of Mercury in Ayurveda and Indigenous Medicine: A Review Dr. tastelessness. Even after these processes. Department of Dravyagunavignana. which is a classical text on RasaShastra. In order to prepare rejuvenative and aphrodisiac formulations. Sri Lanka Ayurveda is a holistic and divine life science. It was developed by the divine people on comprehension through their deep sense and apposite concentration. effectiveness on dreadful diseases and long shelf life. before their preparation. University of Colombo. Keywords: Rasashasta. mercury. lack of side effects. Sri Lanka Cancer patients often attracted to complementary and alternative medicinal practices due to their natural origin. 666g/mL) was tested (n=50) using brine shrimp lethality assay.OP 09-01: In-vitro antidiabetic activities of Nyctanthes arbor-tristis L flower extract and its fractions GPVCN Nanayakkara. Keywords: Nyctanthes arbor-tristis. 90. increasing free radical scavenging 99 .05) glucose uptake at 1. MRRE contained unsaturated sterols.2-diphenyl-1-picrylhudrazyl DPPH assay (n=3).09%.0% respectively). diabetes and cancer. Both extracts were screened for phytochemicals and confirmed by thin layer chromatography. this study aimed to evaluate the antioxidant and cytotoxicity activities of root extracts. terpenoid and cardiac glycosides were observed in both AFE and fractions.5mg/ml. Total phenols. For both extracts. University of Sri Jayewardenepura. 2015. glucose uptake OP 09-02: Antioxidant and cytotoxic properties of Smilax zeylanica L. University of Sri Jayewardenepura. Furthermore. tannins and polyphenols were detected in both extracts. Doses (1. arbor-tristis using in vitro assays. C Padumadasa. 80. Hence.5 (84%) and 3mg/ml (92%) doses. Both AFE and hexane fraction exhibited a significant (p <0. arbor-tristis exerts its antidiabetic activity via inhibition of α-amylase enzyme and increasing glucose transportation across the cells. 3mg/ml. proanthocynidine. At 1. (Family: Smilaceae) root and rhizomes N Malge. flavonoids were quantified using Gallic acid and quarcetine as the respective standards. The non-polar hexane fraction exhibited higher hypoglycemic activities compared to other fractions. Hence. alpha amylase. Flavonoids. Roots and rhizomes of Smilax zeylanica is an important herb in traditional medicine against arthritis.05) inhibition of α- amylase enzyme was revealed with increasing doses in the AFE while the hexane fraction exhibited the highest inhibition. while cytotoxicty (doses:8. fractionation. 100. Air dried roots and rhizome (RR) were refluxed with water and methanol to obtain aqueous (ARRE) and methanol (MRRE) extracts. DC Peiris Faculty of Applied Sciences. A significant (p <0. The present study attempted to elucidate the in vitro hypoglycemic activity of aqueous flower extract (AFE) and fractions (hexane. aqueous extraction. Saponnins. The present findings confirm that N. Free radical scavenging activity (doses:2-300g/mL) was evaluated by 2. DC Peiris Faculty of Applied Sciences. dichloromethane. ethyl acetate fraction promoted a dose dependent (99%) and methanol and dichloromethane fractions showed a dose independent glucose uptake (79. tri terpenes. Both MRRE and ARRE showed a linear relationship with the total phenol and flavanoid contents with their respect standards.5ml/mg. Phytochemical screening was determined using standard protocols. isolation of responsible compounds is required from hexane fraction. AFE and each fraction (n=6) was subjected to in vitro α-amylase inhibition assay and glucose uptake by yeast cells at 25mM of glucose concentration. 84. and anthraquinones. 5mg/ml) were selected based on the study conducted by Rangika et al. ethyl acetate and methanol) of N. flavonoids. Sri Lanka Boiled flowers of Nyctanthes arbor-tristis L (Oleacea) is used in Sri Lankan traditional medicine to combat diabetes. pinnata as an anti–inflammatory agent in folk medicine. arbor-tristis flowers is known for its anti-diabetic 100 . Sri Lanka 3 Faculty of Applied Sciences. flowers N Dhanushika. cytotoxicity OP 09-03: Bio activity guided identification of an anti-inflammatory compound from Munronia pinnata SD Hapuarachchi1. zeylanica possess potent antioxidant and cytotoxic activities. The eluted fractions were screened for bioactivity using carrageenan- induced acute inflammation in Wistar rats. Keywords: Smilax zeylanica. pinnata in carrageenan-induced. sub fraction MPe 2-1b (55. methanol extracts. roots and rhizomes. A significant (p<0. These results of this study provide the scientific rationale for the use of M. senecrassidiol OP 09-04: Investigation of antimicrobial activities of methanol extract from Nyctanthes arbor- tristis L.7µg/mL) compared to the positive control (Potassium dichromate LC50:32±0. ARRE IC50: 108±1. The Open University of Sri Lanka. zeylanica contains potent cytotoxic properties and verifies its ethno-medicinal value.Sri Lanka 4 Faculty of Natural Sciences.3±2. Anti-inflammatory effect of the isolated MPe 2- 1b (senecrassidiol) also showed the maximum inhibitory effect at the 4th hour (57%). University of Sri Jayewardenepura.02 µg/mL). University of Colombo.59±0.02µg/mL.0µg/mL). Ethyl acetate extract (MPe) was subjected to chromatographic separation using column and thin layer chromatographic techniques. Finally. Sri Lanka 2 Faculty of Medical Sciences. TS Suresh2.53±0. University of Sri Jayewardenepura. This study was designed to evaluate the anti-inflammatory effect of methanol extracts of M. antioxidant. Sri Lanka Nyctanthes arbor-tristis L. WTPSK Senarath3. ARRE LC50:84±0. pinnata was extracted successively with hexane. It is concluded that S. (Family Oleaceae) is commonly known as Night Jasmine is one of highly utlizied traditional medicinal plant in Sri Lanka.activity was observed with increasing concentrations (MRRE IC50: 6. chloroform and methanol at room temperature. The present study revealed that the methoanol extracts of the RR of S. Munronia pinnata (MP) is a highly demanded herb in folklore medicine of Sri Lanka.05) linear relationship was observed in the cytotoxic assay (MRRE LC50: 90. The crude powder of M. anti-inflammatory effect. The methanol extract (MPm) was further partitioned between water and ethyl acetate.8µg/mL.0 mg) was identified as senecrassidiol by extensive chromatographic studies. experimental acute inflammatory in Wistar rat model.2µg/mL) compared to the standard ascorbic acid (IC50: 2. All the tested ethyl acetate fractions of methanol extract were showed a significant inhibitory effect on paw oedema and fraction 2 (MPe 2) exhibited the maximum effect at the 5th hour (67%). C Ranasinghe4 1 Institute of Indigenous Medicine. N. Keywords: Munronia pinnata. D Peiris Faculty of Applied Sciences. University of Sri Jayewardenepura. carrageenan-induced wistar rats. The bioactivities of different parts of the plant have not been studied separately. leaves and roots showed highest anti-diabetic activity and 60 % MeOH fraction of the leaves and flowers showed highest anti-diabetic activity than to standard acarbose. roots. Therefore. Antimicrobial activity against pathogenic fungi (Candida albicans and Candida dubliniensis). aspirin.790). Four concentrations (n=3) of the extract (3. flowers. stems and roots of Scoparia dulcis L SM Silva1. anti-diabetic activity 101 . stems and flowers of Scoparia dulcis L were screened to identify the above activities. anti-inflammatory and antidiabetic activities. 1. PA Paranagama2 1 College of Chemical Sciences. MeOH extracts of flowers. coli respectively. antidiabetic and anti-inflammatory activities of extracts from leaves. This concludes that.563 and 1. Keywords: Scoparia dulcis L. flowers. terpenes and steroids. Dried flowers were grounded and extracted with methanol. HAK Maduranga2. Fractions yielded after the partitioning indicated that CHCl3 and 60 % MeOH fractions of leaves. The crude extracts of each part of Scoparia dulcis L showed significantly high antioxidant activity with lower IC50. The lowest antifungal activity was shown against C. Then each extracts prepared from different parts of Scoparia dulcis L was partitioned into hexane.5 and 1mg/mL). aspirin. antioxidant activity.564 respectively at 3mg/mL which was comparable to the standard antibiotics (RMI= 1. Sri Lanka 2 Department of Chemistry.411 and 1. aureus and E. 2. Nevertheless CHCl3 fractions of the flowers and root samples revealed the activities higher than to standard anti- inflammatory drug. dubliniensis (RMI= 0. 1. Our findings suggest that. N. methanolic extract.766). albicans (RMI= 0. Scoparia dulcis L having high medicinal potential in terms of its antioxidant. At 3mg/mL. Vancomycin (for Gram-positive bacteria) and Gentamycin (for Gram- negative bacteria) were used. stems and roots of Scoparia dulcis L using antioxidant. chloroform and 60% MeOH and bioactivities of each fraction obtained was evaluated again using specific bioassays.610). The zone of inhibition was measured and Relative Magnitude of Inhibition (RMI) were determined.pneumoniae (RMI= 0. Sri Lanka Scoparia dulcis L is a valuable medicinal herb widely found in tropical and subtropical countries including Sri Lanka and rich in bioactive compounds such as flavones. Institute of Chemistry Ceylon. Keywords: Nyctanthes arbor-tristis. 2. antimicrobial activity. University of Kelaniya. aeruginosa and S. Gram- positive (Staphylococcus aureus and Methicillin-Resistant Staphylococcus aureus) and Gram-negative (Pseudumonas aeruginosa.and antioxidant activities. aureus (RMI= 1. MeOH extracts of roots and flowers of Scoparia dulcis L exhibited significantly high anti- inflammatory activity than the standard anti-inflammatory drug.5.660) and was comparable to K . agar well diffusion method OP 09-05: Phytochemical screening and study of antioxidant.196 RMI values were shown against Methicillin. But its antimicrobial potential is not known.Resistant S. The extract showed the highest activity against pathogenic bacteria P. the main objectives were to evaluate bioactivities of the leaves. dimethyl sulfoxide (negative control). anti-inflammatory activity. Among two pathogenic fungi the strongest activity was shown against the C. Escherichia coli and Klebsiella pneumoniae) were assessed using agar well diffusion method. anti-inflammatory and anti-diabetic bioassays separately. The present study was undertaken to investigate the antimicrobial activity in lieu of developing antimicrobial drug against increasing drug resistant pathogens. roots and stems samples showed significantly high antioxidant activities. arbor-tristis could potentially useful for the development of therapeutic agent against antimicrobial several important human pathogens. Methanol Extracts of leaves. cheap and natural antibacterial drugs. Sri Lanka 2 Department of Chemistry. which are mediated for their antibacterial property. The secondary metabolites were extracted using sequential extraction method and column chromatography. Further studies are recommended to investigate phytoconstituents of the extracts. respectively.18 mg/ml whereas 43. The objective of this research was to find the antibacterial and anti-inflammatory activity in secondary metabolites of lichen Roccellamontagneicollected from Puttalam lagoon. aureus were observed for 100mg/mL methanolic and aqueous leaf extracts. acetone and hexane extracts of the Talinum paniculatum leaf and Aegle marmelos flower against Escherichia coli (ATCC 25922) and Staphylococcus aureus (ATCC 25923). The crude extracts namely hexane. Sri Lanka Antibacterial resistance has become a serious public health burden at present. The results revealed that each leaf extract of T. General Sir John Kotelawala Defence University. Faculty of Science. methanol. Additionally. All six fractions showed satisfactory results compared to the standard. Statistical comparisons were made using Duncan's new multiple range test. except hexane extract. Aegle marmelos. Institute of Chemistry Ceylon. respectively. paniculatum. Sri Lanka There is evidence that lichens have been used as traditional medicine since early human civilization. It is concluded that T. marmelos. present study evaluated the in vitro antibacterial activity of aqueous. marmelos are promising sources to develop potent. Besides. aureus. The antibacterial properties were tested for crude extracts on four non-pathogenic bacteria namely 102 . coli and S. 2 1 College of Chemical Sciences.OP 09-06: Evaluation of in vitro antibacterial activity of different extracts of Talinum paniculatum and Aegle marmelos RNN Gamage. all leaf extracts of T. paniculatum and flower extracts of A. Materials and Agar well diffusion method and macro-dilution broth technique were performed to determine the zone of inhibition and minimum inhibitory concentration (MIC). HAK Maduranga2. Sri Lanka. the largest zones of inhibition against E. University of Kelaniya. KDKP Kumari Department of Basic Sciences. coli. MIC OP 09-07: Anti-bacterial and anti-inflammatory activity of secondary metabolites of Roccella montagnei N Jayasooriya1. coli and S. KB Hasanthi. There is a necessity and great demand for development of novel efficacious plant based natural antibacterial agents in order to combat against the emergence of antibiotic resistance. Therefore. PA Paranagama1. For T. aureus. exhibited antibacterial potential against S. paniculatum and flower extract of A.69% at a concentration of 0. Keywords:Talinum paniculatum. paniculatum and A. marmelos expressed the largest zones of growth inhibition against E. The dichloromethane extract was partitioned using a silica gel column and six major fractions were obtained. marmelos exhibited antibacterial activity against E. 100mg/mL aqueous flower extract of A.84% was observed for Aspirin at the same concentration. dichloromethane and methanol fractions were tested for anti-inflammatory activity and all fractions showed fairly higher results than the standard. The highest percentage inhibition was observed for the dichloromethane extract which was 92. Faculty of Allied Health Sciences. antibacterial activity. Results show that PPK decoctions and its individual plants S. University of Peradeniya. bacterial resistance pathogens 103 . Five major fractions were obtained by subjecting the dichloromethane extract to column chromatography. the growth of pathogens was compared with the control. is an internal medicine in the Siddha medical system. 10 were shown to have inhibitory activity. Escherichia coli. C.3±2. The dichloromethane crude extract showed satisfactory results against the above-mentioned bacteria species showing the highest activity against Staphylococcus aureus giving an inhibition zone of 18 mm at a concentration of 6.5 – 16. aureus NCTC and 5 strains of MRSA. Plates were incubated at 37 °C for 24 h and the zone of inhibition (ZOI) measured.Staphylococcus aureus. fragrans leaf possess inhibitory activity against both sensitive and resistant strains of S. E. Keywords: antibacterial activity. Malabe. The F4 fraction showed fairly satisfactory results against three bacteria species. The antimicrobial activity of PPK has not been reported previously. After 24 hours incubation.66 mg/ml. Escherichia coli NCTC 10418. particularly for the treatment of infections by bacteria resistant pathogens. ribes. anti-bacterial. MRSA. Sri Lanka 3 Industrial Technology Institute. Parankipaddaikudineer (PPK) consists of 13 plant parts from 12 medicinal plants and is used to treat skin diseases. aromaticum. a hot water extract. Parankipaddai Kudineer. Faculty of Medicine. 1/20. anti-inflammatory.0±1. 1/40. Bacillus subtilis and Staphylococcus epidemics. aspalathoides and M. I. Pseudomonas aeruginosa NCTC 10662 and 5 clinical isolates of Methicillin-Resistant S. Antibacterial screening at 1/10. Staphylococcus aureus NCTC 6571. The study of the decoction as well as its component plants is timely as there is a rapidly increasing demand for novel antimicrobial agents from plant sources. All fractions gave satisfactory results for antibacterial assay. 9 of which showed activity against S. Sri Lanka Decoction. Keywords: secondary metabolites. It was concluded that secondary metabolites of lichen Roccellamontagnei possess anti-inflammatory and antibacterial activities. aureus were used in this study. University of Jaffna. 1/80 and 1/160 dilutions of decoction was performed using a well diffusion method. Out of 12 individual plant decoctions. angustifolia. Therefore the F4 fraction was subjected to a preparative thin layer chromatography and one fluorescent-active compound was isolated. V Thevanesam2.0 mm) against S. Roccellamontagnei. S Kathirgamanathar3 1 Unit of Siddha Medicine. The decoction was prepared according to the method in Siddha literature. lichens OP 09-08: Antibacterial activity of Parankipaddai Kudineer (decoction) and its component plants T Thileepan1. PPK decoction has inhibitory activity (ZOI: 15. Ten µL of the 1/10 dilution of each test strain was used. aureus NCTC 6571 and 5 MRSA strains at 1/10 dilution. aureus at a high dilution which indicates the potential of using them as antimicrobial agents. Three replicates were carried out for the entire procedure. Sri Lanka 2 Department of Microbiology. The Minimum inhibitory concentration (MIC) was determined by the agar dilution method. OP 09-09: Hepatoprotective effects of Osbeckia octandra on experimental liver cirrhosis BRS Bogahawaththa1, P Weththasinghe2, SP Kodithuwakku1, W L Dissanayake3, EH Siriweera4, CB Herath5, RRMKK Wijesundara6, DPIN Pathirana2, R P V J Rajapakse6, MPB Wijayagunawardane1 1 Department of Animal Science, Faculty of Agriculture, University of Peradeniya, Sri Lanka 2 Department of Animal Science, Faculty of Agriculture, University of Ruhuna, Sri Lanka 3 Faculty of Dentistry, University of Hong Kong, Hong Kong SAR 4 Department of Pathology, Faculty of Medicine, University of Peradeniya, Sri Lanka 5 Department of Medicine, The University of Melbourne, Austin Health, Heidelberg, Victoria , Australia 6 Department of Veterinary Pathobiology, Faculty of Veterinary Medicine & Animal Science, University of Peradeniya, Sri Lanka Natural plant extracts are frequently screened for new drug discoveries. Sri Lankan traditional medicine widely uses the leaves of a native plant, Heen Bowitiya (HB; Osbeckia octandra) for treatment of human liver diseases. Therefore, the present study was focused to evaluate the hepato-protective effects of HB leaves against Thioacetamide (TAA) induced liver toxicity in Wistar rats aged 5 months (250 g). Four randomly assigned groups of rats received either TAA (100 mg/kg BW, intraperitoneal, twice a week), leaf extract of HB (500 mg DM/kg BW, oral gavage, twice a week), TAA + HB or equal amounts of distilled water orally. Samples were collected at 5, 10 and 15 weeks after the first treatment, for biochemical, histopathological and gene expression analysis. Aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (AP) for liver function, and creatinine for kidney function were significantly (p<0.05) elevated in TAA administered rats. Interestingly, HB treatment significantly (p<0.05) decreased these parameters in TAA administered rats compared to those receiving TAA alone. The above findings were confirmed by histological assessments using H&E and Masson's trichrome staining. Moreover, HB treatment markedly ameliorated the expression of collagen 1, TNF-alpha, TGF-beta1, alpha-SMA and VEGF-R1 genes in TAA administered rats compared to those receiving TAA alone. Furthermore, human vascular endothelial cell based angiogenic assay revealed significant anti-angiogenic effects of HB. We conclude that HB leaf extracts protect the liver from toxic injury induced by TAA but the therapeutic potential of HB leaves needs to be further investigated. Keywords: liver injury, Osbeckia octandr, Thioacetamide and rats OP 09-10: Preliminary screening of bioactive secondary metabolites produced from endolichenic fungi Xylariapsidii from the mangrove lichen, Pyrenulaochra ceoflava in Puttalam District, Sri Lanka DVH Dharmakeerthi1, HAK Maduranga2, PA Paranagama2 1 College of Chemical Sciences, Institute of Chemistry Ceylon, Sri Lanka 2 Department of Chemistry, University of Kelaniya, Sri Lanka Endolichenic fungi are microorganisms living in the thalli of lichens that are analogous to the plant endophytic species inhabiting the intercellular spaces of the hosts. Endolichenic fungi live within the lichen thallus much the same way as endophytes live between cells in plant tissues. In this study the endolichenic fungus Xylariapsidii from the lichen Pyrenulaochra ceoflava occurring in the mangrove plants in Puttalam District, Sri Lanka was investigated for its biological activities. The fungus was grown in large PDA plates and incubated at room temperature for ten days and the secondary 104 metabolites were extracted into ethyl acetate. The crude extract was then subjected to anti- inflammatory, antioxidant and antibacterial assays. The concentration series of crude extract showed moderately low anti-inflammatory activity for the HRBCs Membrane Stabilization assay compared with the standard Aspirin used. The crude showed considerably high antioxidant activity in DPPH assay and showed very high antibacterial activity against the three bacteria strains used in the Agar Well Diffusion assay compared with the standard positive Azithromycin used. Since the antibacterial activity was high, the crude was partitioned with hexane, chloroform and 60% methanol to obtain hexane, chloroform and methanol fractions respectively and each fraction was evaluated for their antibacterial activities. The antibacterial activities as well as the Minimum Inhibitory Concentrations of each portion were identified using Agar Well Diffusion assay. The chloroform fraction that showed the highest activity was further fractionated using bio assay guided column chromatography and preparative TLC to isolate the compounds present. Keywords: Endolichenic fungus, Pyrenulochra ceoflava, Xylariapsidii, anti-bacterial activity, antioxidant activity, anti-inflammatory activity OP 09-11: In-vitro anti-inflammatory properties of Finger millet (Eleusine coracana) varieties from Sri Lanka SAS Jayawardana1, JKRR Samarasekera1, GHCM Hettiarachchi2, J Gooneratne1 1 Industrial Technology Institute, Sri Lanka 2 Department of Chemistry, Faculty of Science, University of Colombo, Sri Lanka The prevalence of inflammatory mediated diseases is increasing worldwide. Arachidonate 5- lipoxygenase (A5-LOX) is a key enzyme involved in biosynthesis of potent mediators of inflammatory disorders and allergic reactions. Hyaluronidase leads to degranulation of mast cells, causing the release of inflammatory mediators. Due to the formation of reactive oxygen species during its catalytic action, Xanthine oxidase (XO) is responsible for inflammatory diseases. Therefore, inhibitors of A5-LOX, hyaluronidase and XO play a vital role in the treatment of inflammatory mediated diseases. The present study was conducted to evaluate in vitro anti-inflammatory properties of Sri Lankan finger millet extracts. Flours of whole grains of Ravi, Rawana and Oshadha finger millet varieties, were extracted with ethanol and methanol separately and used in ev aluating A5-LOX, hyaluronidase and XO enzyme inhibitory properties and 1,1-diphenyl-2-picryl-hydrazyl (DPPH) and 2- azino-bis (3-ethylbenzothiazoline-6-sulfonic) acid (ABTS+) radical scavenging properties. Data of each experiment were statistically analysed. The results showed significant differences (P<0.05) among three varieties and between the extracts. Among all extracts, methanolic extract of Oshadha exhibited the highest anti-A5-LOX activity (IC50 = 484.42 ± 5.54 µg/ml) and anti-XO activity (IC50 = 764.34 ± 8.47 µg/ml). All extracts showed less than 50% hyaluronidase inhibitory activity at 1000 µg/ml concentration. Positive correlations between IC50 values of A5-LOX and XO inhibitory activities and DPPH and ABTS+ radical scavenging properties indicate scavenging radical intermediates as the prominent mechanism of anti-A5-LOX and anti-XO inhibitory activities of the extracts. This is the first report revealing anti-A5-LOX, anti-hyaluronidase and anti-XO properties of any extract of Sri Lankan finger millet varieties. Keywords: Arachidonate-5-lipoxygenase, Finger millet, Hyaluronidase, Radical scavenging properties, Xanthine oxidase 105 OP 09-12: Antioxidant properties of lime juice extract MRDA Chandrasiri1, MRTB Chandrasiri1, RDPA Dissanayaka2 1 Virgen Milagrosa University Foundation, Philippines 2 University of Sri Jayewardenepura, Sri Lanka Antioxidants are widely used in dietary supplements and have been investigated for the prevention of diseases such as cancers. This study was conducted to determine the antioxidant properties of lime (Citrus aurantifolia) juice extract claiming the therapeutic effect on possible active constituents present on the said plant. The research design covered both qualitative and quantitative experimentation. The experiment aimed to identify the antioxidant activity of the ethanolic extract of Citrus aurantifolia. The experiment was planned with the limitation of experimenting the above mentioned properties of the plant and comparative analysis of the results. To observe the antioxidant activity, 2,2-diphenyl-1-picylhydrazyl(DPPH) assay was conducted. The test was carried for different concentrations of the fruits’ ethanolic extract and Ascorbic acid was used as the positive control. The absorbance readings were measured spectro-photometrically. The readings were interpreted statistically to see if there was any significant difference in the antioxidant activity between the positive control and the Citrus aurantifolia fruit extract. Different concentrations of the ethanolic juice extract of Citrofortunellamicrocarpa show lower rates than the absorbance readings of the controlled reference, ascorbic acid. Due to the color change of the DPPH reagent as well as the percent inhibition of DPPH activity, which gave a positive value approving the inhibition of free radicals in the solution, the conclusion that Citrofortunellamicrocarpa fruit juice possesses antioxidant properties was made. There was no significant statistical different between the mean absorbance readings between Citrofortunellamicrocarpa fruit juice and Ascorbic acid regarding the DPPH assay, which leads to the conclusion that the antioxidant property of any concentration of Citrofortunellamicrocarpa fruit juice is comparable with the positive control used. Keywords: lime juice, Antioxidant, Citrofortunellamicrocarpa, DPPH assay OP 09-13: Antifungal potential of garlic against multidrug-resistant Candida species WAS Wijendra1, KVDHS Karawita2, KMEP Fernando3, P Jayasekera1 1 Department of Mycology, Medical Research Institute, Colombo 8, Sri Lanka 2 Research and Development section, Nature’s Beauty Creation Pvt. Ltd., Sri Lanka 3 Department of Botany, University of Sri Jayewardenepura, Sri Lanka Opportunistic Candida has developed resistance towards antifungal drugs prescribed to treat Candida infections. Today, side effects of some drugs are a major concern in the health sector. This current situation has led to exploration of the potential of herbs as alternative drugs. Garlic (Allium sativum) is used extensively in traditional medicine for many human ailments. This study aimed to evaluate the efficacy of garlic extracts against multidrug-drug resistant Candida strains. The antifungal activity of aqueous and methanol extracts of raw and boiled garlic was assessed against the reference strains; C. albicans (ATCC 90028), C. tropicalis (ATCC 750), C. glabrata (ATCC 90030), C. parapsilosis (ATCC 23019) and resistant Candida isolates from patients using well diffusion method and poison food technique. Inhibition zones were compared with the reference standard 106 ketoconazole. Minimum inhibitory concentrations (MIC) of reference Candida strains and resistant Candida isolates from patients were determined using broth micro dilution method. Ketoconazole (positive) and sterile distilled water and methanol (negative) were used as controls. Both aqueous and methanol extracts of raw garlic showed significantly larger inhibitory zones for four reference strains compared to ketoconazole (p<0.05) while boiled garlic extracts showed significant inhibition on C. parapsilosis. Methanol extracts of raw garlic suppressed growth of all four strains by 100%. MIC of methanol extract of raw garlic for local patient’s strains recorded the range from 0.15 to 0.62 mg/ml. Results revealed that Candida isolates are susceptible to raw garlic extracts which possess antifungal properties. Thus garlic can be developed as an effective therapeutic drug against multidrug-resistant Candida strains. Keywords: candida, garlic, antifungal OP 09-14: Identification of Bergenin and a diastereoisomer of Bergenin from the herb, Flueggea Leucopyrus Willd as potent anti-cancer agents against human ovarian carcinoma VP Bulugahapitiya1, MMAB Munasinghe1, ML Hettihewa2, N Kihara3 1 Department of Chemistry, Faculty of Science, University of Ruhuna, Sri Lanka 2 Department of Pharmacology, Faculty of Medicine, University of Ruhuna, Sri Lanka 3 Department of Chemistry, Faculty of Science, Kanagawa University, Kanagawa, Hiratsuka, Japan Fluggea Leucopyrus Willd. (Euphorbiacea) is a medicinal herb widely available in the dry zone of Sri Lanka and has been utilized as a complementary medicine for cancer. Anti-cancer activity of the plant extracts against different types of carcinoma has been reported and Bergenin has been isolated as an anti-oxidant from the herb. This paper presents the identification of cytotoxic compounds against human ovarian carcinoma from the leaves of F.leucopyrus. Human Ovarian carcinoma cell line (A 2780-Adhesive type) was cultured in RPMI 1640 cell culture media and used for the MTS cell proliferation and HTERT inhibitory assay for determining the anti-cancer activity of F. leucopyrus. As significant inhibition for the cell proliferation was observed with the ethyl acetate extract of the leaves (IC50 36.35 µg/mL), it was purified using column chromatography to obtain about six pure compounds. Out of six, two compounds (compound-1 and compound-2) showed strong inhibition of cell proliferation giving IC50 values of 48.53 and 12.36µg/mL respectively in MTS assay. The cell mortality was identified by staining with the Trypan blue 0.4 % solution. In the HTERT inhibitory assay, only the compound-1 showed the prompt depletion of HTERT content in human ovarian cancer cells (in the 50-200 µg/mL concentration range). The structures of compounds 1 and 2 were elucidated using Mass and NMR spectroscopy. Compound-1 was characterized as the known compound Bergenin and compound-2 was characterized as a stereoisomer of Bergenin at C-9 and C- 14. In conclusion, bergenin (2R,3S,4S,4aR,10bS)-3,4,8,10-Tetrahydroxy-2-(hydroxymethyl)-9- methoxy-3,4,4a,10b-tetrahydropyrano[3,2-c]isochromen-6(2H)-one) and its diastereoisomer (2R,3S,4S,4aS,10bR)-3,4,8,10-tetrahydroxy-2-(hydroxymethyl)-9-methoxy-3,4,4a,10b- tetrahydropyrano[3,2-c]isochromen-6(2H)-one), present in the leaves of F. leucopyrus, are likely to be potent anti-cancer compounds. Keywords: F. leucopyrus, Bergenin, diastereoisomer of bergenin, human ovarian carcinoma 107 LDAM Arawwawala3 1 Institute of Indigenous Medicine. TI possesses hypolipidaemic. (Family: Euphorbiaceae) (TI). Pre-treatment with either the extract of LLC or silymarin showed only early pathological alterations of cell injury. University of Colombo. Glycyrrhiza glabra. Sri Lanka 2 General Sir John Kotelawela Defense University. among others. The survivors in pre-treated groups showed reduction in elevation of serum alanine aminotransferase and aspartate aminotransferase levels compared to the survivors of the pathological control group (p<0. anti-cancer and anti-diabetic actions. in Streptozotocin induced diabetic rats MS Pallie1. PCM intoxicated animals showed centrilobular necrosis involving approximately 5% of the liver parenchyma. Industrial Technological Institute. hepatoprotective activity. These results indicate that extract of LLC increased the percentage of survival in mice against PCM induced hepatotoxicity at a dose of 80 mg/kg body weight. Piper longum. silymarin OP 09-16: Evaluation of anti-diabetic activity of Tragia involucrata L. Type 2 diabetes 108 . and two other doses of 875 mg/kg and 1100 mg/kg. Osbekia octandra. Curcuma longa. However. AM Abeysekera1 1 Depatment of Chemistry. Silymarin was used as the positive control. The same dose of PCM killed only 25% in LLC pre-treated group at the dose level of 80 mg/kg. The present study investigates the anti. Malabe. Tinospora codifolia. Nugegoda. University of Sri Jayewardenepura. University of Sri Jayewardenepura. B Senevirathna3. Boerrhavia diffusa. University of Sri Jayewardenepura. T. Sri Lanka 2 Biochemistry. Sri Lanka 3 Depatment of Pathology. Nugegoda.05). C Padumadasa1. Keywords: Polyherbal formulation. Hepatoprotective activity of the isopropyl alcohol:water (70:30 v/v) extract of LLC (80 and 160 mg/kg) was studied against paracetamol (PCM) induced hepatotoxicity in female mice at the Institute of Cancer Research (ICR). UG Chandrika2. Treatment related change in serum alkaline phosphatase and total bilirubin were not observed against PCM induced hepatic damage. The lethality is the same as the pathological control group (PCG) at the dose level of 160 mg/kg. bellerica. with pre-treatment with silymarin at the dose level of 50 mg/kg. Tephrosia purpurea and Vernonia cinerea was formulated by an expert panel of Ayurvedic physicians at Link Natural Product (Pvt) limited as a hepatoprotective preparation. Nugegoda. P. PK Perera1. paracetamol. CL Goonasekera2. Sri Lanka Link LivecareTM (LLC) consisting of 14 herbs: Andrographis paniculata.OP 09-15: Hepatoprotective activity of Linklive CareTM against Paracetamol induced hepatotoxicity in female mice KAAU Karunarathna1. Terminalia chebula. emblica. only 25% of the animals survived. is known as Welkahambiliya in Sri Lanka and is a medicinal herb used in the Sri Lankan traditional medical system and Ayurveda. anti-inflammatory. Phyllanthus amarus. Sri Lanka Tragiainvolucrata L. analgesic.diabetic effect of TI hot water extract (HWE) using the therapeutic dose of 550 mg/kg. Eclipta alba. Single oral administration of PCM (500 mg/kg) induced 50 % lethality in mice in the pathological control group after 24 hr. KMN Kumarasinghe2. Sri Lanka 3 Research and Development Complex. Groups I to V were orally treated with distilled water (1 mL/day). three drug treated groups (200. albiflora has anti-inflammatory activity by using formaldehyde-induced paw oedema in Wistar rats. control (water). albiflora showed low anti-inflammatory activity on cotton-pellet induced granuloma test. under same vernacular name three plants are being used. and standard group (indomethacin: 4 mg/kg) for 7 consecutive days. It can be considered as a controversial medicinal plant. Wistar female rats (160 . 7. 2017 from Sabaragamuwa area. prostaglandin synthesis was not inhibited significantly by the C. ethics committee and conducted in accordance to the WHO guidelines. HWE of TI at a dose of 550. one group (n=6) of normo-glycaemic rats were orally given distilled water (1 mL/day) as a negative control group.mellitus was induced for adult male Wistar rats by providing 60% high fat diet for 21 days. on formaldehyde induced paw oedema in rats HMIC Herath. type 2 diabetes mellitus Op 09-17: Anti-inflammatory activity of Curcuma albiflora Thw. Inflammations were induced by 0. The drug group (400 mg/kg) significantly (P<0. 1100 mg/kg/day for 28 days respectively. TDCMK Wijayasiriwardene. Thirty rats were assigned into five groups. a dose of 550 mg/dL is more efficacious in causing anti-diabetic effect compared with the other two doses of 875 and 1100 mg/dL in diabetic induced adult male Wistar rats. Curcuma albiflora. Whole plant extract was prepared using ethanol and water (1:1) by continuous extraction (6 h). Rats with blood glucose levels higher than 175 mg/dL were selected as diabetic rats and divided into 5 groups (n=6). GAS Premakumara Industrial Technology Institute. 875. extract. In conclusion. Colombo were used. is unexplored. anti-inflammatory 109 . and 600 mg/kg). Plants were collected in March. and administering freshly prepared Streptozotocin (30 mg/kg/rat) on the 22nd day.05) reduced the paw oedema from the day 5 to 7 when compared with the control. 150 mg/kg Metformin/day. Further. albiflora Thw. 400. Current study was launched to scientifically test none evidence based traditional claim made that C.250 g) from MRI. Voucher specimens were authenticated from National Herbarium. An endangered endemic variety of Curcuma albiflora Thw. Keywords: Tragiainvolucrata. Peradeniya. Keywords: Formaldehyde induced paw oedema. Sri Lanka Harankaha is an important medicinal plant. anti-diabetic. Fasting blood glucose was measured on 0. 21 and 28 days. Results showed that all the doses of HWE of TI and reference drugs significantly reduced high blood sugar levels and the dose of 550 mg/dL showed the most effective anti-diabetic effect compared with other doses. which is being used for anti-inflammatory disorders. and anti-inflammatory activity could be related to other autacoids. Since previous study reveals that C. The study procedures and ethical clearance were approved by the University of Colombo. 14.1 ml of 2% formaldehyde in distilled water into the plantar oponurosis of the left hind paw on the day 1 and 3 under anesthesia. Overnight fasted healthy male wistar rats (12 h) were randomly divided into four groups (n=6/group).05.65.OP 09-18: Evaluation of hypoglycaemic activity of a medicinal plant extract mixture in healthy rats DSNK Liyanagamage.05).816. The present study investigated the effects of GC on blood glucose and serum lipids in Wistar rats. cloves of Allium sativum (L). Compared with baseline levels. The results revealed that the administration of the combined plant extract mixture showed dose dependent hypoglycaemic effects in healthy rats and further studies are warranted in diabetic rats. Results were analyzed using SPSS version 16. 1. -13. University of Kelaniya. virgin coconut oil (VCO).08.34%) in healthy rats (p< 0.87. -30.05) were observed between groups on oil only diets or between groups fed with diets containing mixtures of oil+ GC. The equal amount of each above plant parts (100 g) were mixed and extracted in to cold water.0 (equivalent human therapeutic dose) and 1. triglycerides -31. 6.5. Although statistically not significant.1.32. mean serum glucose levels decreased in all four groups.). -20. 8.5.05) only in rats fed with mixtures of oil + GC (groups 3 and 4).8 and high density lipoproteins (HDL) -1.0 g/kg showed a significant improvement on oral glucose tolerance as water: acetone extract (14. -8. and -41. Faculty of Medicine.1%).11. Group one rats received distilled water (control). WCO + GC (1:1 ratio) and VCO + GC (1:1 ratio) respectively. Sri Lanka Polyherbal therapy used in traditional medicine has been offered a promising key in the management of diabetes mellitus since antiquity. Compared with the baseline. fruits of Garcinia queasita Pierre and seeds of Piper nigrum(L) in healthy Wistar rats in order to scrutinize the therapeutic use in Sri Lankan traditional Ayurvedic medicine. diets with oil + GC mixture resulted 110 .5 g/kg. Group two. blood glucose and serum lipids were assayed.85 mg/dl respectively. Sri Lanka Grated coconut (GC) is widely used in traditional cooking purposes to make “sambal “and traditional sweets. three and four administered cold water. Faculty of Medicine. V Karunarathne. The decrease was significant (p<0. -49.88. hot water (3h. No significant differences (p>0.1. mean differences in serum lipids in mg/dl were.82 in groups 1-4 respectively. AP Attanayake. University of Peradeniya. GC is rich in dietary fiber (9%).37%) and cold water extract (6. After 36 weeks of feeding.75. S Chakrawarthy2. Lunuwila. hot water and water: acetone extract mixture respectively at the doses of 0. refluxed) and water : acetone mixture (1:1 ) separately. containing white coconut oil (WCO). S Jayasinghe Faculty of Science. Four groups of male Wistar rats (1-4) were maintained on four iso-caloric test diets. total cholesterol (TC) 9. Sri Lanka 2 Department of Biochemistry. -10.47. hypoglycaemic activity OP 09-19: Effects of grated coconut kernel on blood glucose and serum lipids in Wistar rats HAE Samaranayake1. University of Kelaniya. -41. The plant extract treated rats at the dose of 1. The present study was carried out to evaluate the hypoglycaemic activity of a medicinal plant extract mixture composed of leaves of Murraya koenigii (L. The acute hypoglycaemic effect was evaluated over a four hour period using total area under the curve method. Sri Lanka 3 Department of Public Health. Keywords: plant extract mixture. hot water extract (10.0 by ANOVA. healthy rats. 0. AR Wickremasinghe3 1 Coconut Processing Research Division. Coconut Research Institute.22. -7. G3 was given seed lipids (100µl/day) for 3 months. K Wijesundera1. AJ Hilmi3. The present study was performed to evaluate inhibitory effects of α-ESA rich bitter melon lipids on hepatocellular carcinoma (HCC). Induction of HCC resulted in behavioral changes. G1 was not given any treatment. biochemical (SGOT/SGPT/Creatinine) and histopathological (size of neoplastic foci) investigations. dietary fiber. reduction of body weights and elevations of SGOT. Ethical approval was received by the ethical review committee of the Faculty of Veterinary Medicine and Animal Science. IPGHU Dissanayake2. α-Eleostearic acid. After the HCC induction. Faculty of Veterinary Medicine and Animal Science. The reduced size of neoplastic foci of G3 compared with G2 was further confirmed by histopathology. The current finding further supports the anti-cancer effects of α-ESA rich bitter melon lipids. samples were collected for haematological (WBC/DC). which is reported with a potent anti-carcinogenic property. Keywords: hepatocellular carcinoma. HCC was induced in G2 and G3 by intra-peritoneal injection of DEN (30mg/kg) followed by 6 weeks oral treatment of TAA (45mg/kg). AP Jayasooriya2. bitter melon. hypoglycemic action OP 09-20: Inhibitory effects of α – Eleostearic acid (α-ESA) rich bitter melon seed lipids on hepatocellular carcinoma in rats KNK Ranasinghe1. Hepatic enlargements with nodules were observed in G2 and G3. Faculty of Medicine. However. anticarcinogenic 111 . University of Peradeniya. Lower TC and higher HDL levels were observed in rats fed with VCO containing diet. feed and water intake was monitored. AD Premarathna1. SAM Kularatne4. Teaching Hospital Kandy.in a greater reduction of serum triglycerides. After sacrificing. University of Peradeniya. The appearance of liver samples in G1 was small and without nodules. Sri Lanka 2 Department of Basic Veterinary Sciences. Sri Lanka 3 Cancer Treatment Centre. RPVJ Rajapakse1 1 Department of Veterinary Pathobiology. the appearance of nodules in G3 was less intensive as compared with G2. Inclusion of grated coconut in the diet resulted in significant hypoglycemic action and a possible hypolipidaemic effect in Wistar rats. 8 week old female rats were divided into 3 groups as G1. and further studies are highly warranted for exploration of possible mode of action and further human clinical trials. University of Peradeniya. Behavioral changes. Keywords: blood sugar. Sri Lanka The seed lipids of bitter melon (Momordica charantia) contain a conjugated trienoic fatty acid called α-Eleostearic acid (α-ESA). Faculty of Veterinary Medicine and Animal Science. SGPT levels. University of Peradeniya. Sri Lanka 4 Department of Medicine. G2 and G3. As such. Faculty of Allied Health Sciences. this study investigated the hypoglycaemic properties and toxicology of hot water extract (HWE) of A. Sri Lanka 3 Department of Basic Sciences. Sri Lanka 3 Medical Research Institute.0. Sri Lanka 2 Pharmacy Unit. VCO (4 %) and WCO (4 %). Sri Lanka. Studies have demonstrated a protective effect of some dietary oils against NAFLD. random (n=6) and post-prandial (n=6 per group/after a 5 ml/kg 50% oral glucose) 112 . p<0. VCO (2 %). Sir John Kotelawala Defense University. Sri Lanka 4 Department of Biochemistry. Sri Lanka According to Sri Lankan folk medicine. WD Ratnasooriya3 1 Department of Chemistry. Faculty of Medicine. lipid peroxidation. Due to its high antioxidant potential. 750 and 1000 mg/kg) or 1 ml distilled water (negative control) or 22. rats were euthanized and livers were collected.7±6. The rats on 4% WCO showed significant hepatic steatosis compared with 4% VCO diet (27. In comparison with 4% VCO. Five groups of male Wistar rats (5 rats/ group) were maintained on five test diets containing soya oil (2 %) as control. Faculty of Medicine. VCO exerts a protective effect on the liver by reducing lipid peroxidation and steatosis in Wistar rats. MDJ Wijayabandara2. The present study investigated the effects of virgin coconut oil (VCO) and white coconut oil (WCO) on hepatosteatosis and lipid peroxidation in Wistar rats. University of Colombo.05). WCO (2 %). Faculty of Medical Sciences. University of Sri Jayewardenepura. Quantitative and qualitative aspects of dietary fat influence triglyceride deposition in the liver and the progression to NAFLD. Sri Lanka and Institute of Nanotechnology.5 % vs 3. Different doses of HWE (500.9±2. PB Galhena4. Keywords: non-alcoholic fatty liver disease. Sri Lanka Hepatic steatosis and oxidative stress are the main mechanisms in the pathogenesis of non-alcoholic fatty liver disease (NAFLD). p<0. S Chackrewarthy4 1 Coconut Processing Research Division. Sri Lanka 2 Department of Pathology. University of Kelaniya.05).05).7 vs 23. RMUS Ratnayake2. VCO could be effective in the treatment and/or prevention of hepatosteatosis.9±2. In conclusion. Data were analysed by ANOVA using SPSS. mature leaves of Adenanthera pavonina is claimed to possess blood glucose lowering potentials. 4% WCO resulted in a significantly higher level of lipid peroxidation (14.7±1.7 vs 14.OP 09-21: Protective effects of virgin coconut oil against Hepatosteatosis and lipid peroxidation in Wistar rats HAE Samaranayake1. Colombo. University of Kelaniya. Faculty of Science. antihyperglycaemic and toxicological evaluation of aqueous leaf extract of Adenanthera pavonina (Fabaceae) in rats RK Dissanayake1. Lunuwila. p<0.1 %. pavonina mature leaves using rats. Hepatosteatosis OP 09-22: Hypoglycaemic.5 mg/kg of tolbutamide (positive control) were orally administrated to normo-glycaemic rats and their fasting (n=6 per group). R Karunakaran3. With the increase of VCO from 2 to 4%.7. After 22 weeks. hepatic MDA levels significantly decreased (18. Coconut Research Institute. Coconut oil is a widely used dietary oil with applications in traditional medicine. Lipid peroxidation by TBARS assay as malonaldehyde (MDA) nmol/g protein and histopathological assessment for the degree of steatosis was done. the present study was conducted to investigate the morphological. Sri Lanka Sabaragamuwa University of Sri Lanka Halosarcia indica (Willd. Halosarcia indica. total phenol content. Dissanayaka. there is an immense potential of use of H. P K Dissanayake Industrial Technology Institute. Total antioxidant capacity (TAC) was determined by 1. lead and arsenic were detected in any maturity stages.A potential herbal salt production plant RM Dharmadasa. up to 2 h) and fed (only mid dose tested. diabetes. up to 2 h) rats. Since the plant contain the higher content of sodium and free from heavy metals. phytochemical analyses were carried out as described by WHO standards. and antihyperglycaemic effect (all doses. Keywords: Ameranthaceae. 113 . Sri Lanka OP 09-23: Phytochemical.) Paul G. total antioxidant capacity. salt tolerant plant distributed in coastal areas in different parts of the world. physico-chemical. physicochemical and bioactivity of Halosarcia indica (Willd. Keywords: A.) Paul G. Total phenol content (TPC). All physicochemical parameters were in acceptance levels. up to 3 h following glucose challenge). metal content and bioactivity of Halosarcia indica. total flavonoid content. Oxygen Radical Absorbance Capacity (ORAC) Assay and Ferric Reducing Antioxidant Power (FRAP) Assay. hepatotoxicity (SGOT and SGPT).Wilson (Amaranthaceae) is a succulent. total flavonoid content (TFC) were carried out by previously published protocols. I. R. Therefore. DPPH and ORAC assays were varied among different maturity stages. Acute and sub chronic (21 days) toxicities were evaluated including overt signs of toxicity.serum glucose levels were determined at hourly intervals for 4 hours using standard procedures. indica as an herbal salt in coming future. TAC of ethanolic and aqueous extracts of different maturity stages tested by FRAP. neurotoxicity (bridge and bar test) and nephrotoxicity (serum creatinine and urea). Except for the production of watery stools.Wilson (Amaranthaceae) .09%) content and interestingly no cadmium. Even though it has been used as a food ingredient in many countries. Physicochemical. 1-diphenyl-2-picryl-hydrazyl (DPPH) Radical Scavenging Assay. phytochemical. P Ranasinghe. scientific information on bioactivity and other pharmacognostic parameters are scattered or lacking. antihyperglycaemia.05) hypoglycaemic effects in both fasted (all 3 doses. pavonina. It is concluded that A. the extract was well tolerated without significant toxicity. The results showed that HWE of leaves possesses significant (P < 0. The plant exhibited the higher sodium (11. pavonina leaves exhibit safe oral hypoglycaemic and antihyperglycaemic activities supporting its use in Sri Lankan folk medicine in the treatment for diabetic conditions. hypoglycaemia. Sri Lanka and Center for biomolecular sciences. Clonogenic. LJPAP Jayasooriya3. Previous research has shown that certain plant species in the Apocynaceae family possess anticancer activities. Finely powered dried leaves and stems were subjected to ethanol extractions.0001). ESEM demonstrated morphological differences in treated SKBR3 cells. Caulerparacemosa. Faculty of Veterinary medicine and Animal Science.University of Nottingham.04). Sri Lanka. Sri Lanka and International Atomic Energy Agency (IAEA) Vienna. Ethical approval was obtained from the Department of Forest. Clonogenic assays showed low colony formation (P≤0. UK Plants play an important part in human health. Sri Lanka. These results are promising for HER2 positive breast cancer. University of Peradeniya. MTT assay was performed on primary mice fibroblasts and HeLa 114 . University of Nottingham. L Turyanska. Gracilariacorticata and Sargassumillicifolium by in-vitro studies TH Ranahewa1. stem extract: 12. Leaf extract was able to generate high levels of ROS (p0. Confocal microscopy. > 50% of drugs used in cancer chemotherapy are natural-product derived. M Al-Hayali. the anticancer effect of leaves and stems of Tabernaemontana divaricate were investigated. Faculty of Veterinary medicine and Animal Science. The current study was conducted to determine the anti-cancer properties of three selected seaweed species (in-vitro). Sri Lanka The incidence of cancer has increased steadily and it is a debilitating disease. Methods.72. MTT. Further investigations are being conducted to isolate chemical compounds from this plant species for cancer therapy.001). RRMKK Wijesundera1. Austria 3 Department of Basic Veterinary Sciences. Sri Lanka 2 Department of Veterinary Pathobiology. RPVJ Rajapakse1 1 Department of Veterinary Pathobiology. UK 2 Nanoscale and Microscale Research Centre . KJK Karunathilake1. TD Bradshaw 1 Faculty of Medical Sciences. Environmental scanning electron microscopy (ESEM) were used to determine activity. University of Nottingham. i. HER2 + SKBR3 cell line showed the best sensitivity (GI50 -leaf extract: 6.vitro assays OP 09-25: Evaluating the anti-cancer properties of aqueous extracts from three seaweeds namely. Apoptosis. V Wijewardana2. Slight amounts of apoptotic cells with leaf and stem extracts were found (p≤0. Both extracts showed low SKBR3 cell migration (p≤0. Further. Therefore. Seaweeds are reservoirs of bioactive compounds and are utilized as herbal medicine to treat many diseases in many countries.01). University of Sri Jayawardenepura.0001). divaricate were collected from Colombo. AD Premarathna1. The use of plant based natural products for the treatment of cancer has been prevailing. Gamma H2AX. UK 3 Faculty of Science. L Zhang. Keywords: natural products.89 µg/ml ± 1.49 µg/ml ± 4.Leaves and stems of T.01). the leaf extract caused minor amounts of DNA double strand breaks by gamma H2AX analysis (p≤0.0001). Reactive oxygen species (ROS).OP 09-24: Tabernaemontana divaricate illustrating potent anticancer activity in HER2 positive SKBR3 breast cancer cells AI Kuruppu1. N Weston. University of Peradeniya. Cell migration. University of Peradeniya. Faculty of Veterinary medicine and Animal Science. Currently. C Parmenter. Both leaf and stem extracts caused G1 arrests in cell cycle analysis (p≤0. breast cancer. Cell cycle. PA Paranagama1 1 Department of Chemistry. KH Tennakoon2. which employs a protein binding amino xanthene dye. crude extract of selected fungal isolate was evaluated for the anticancer activity against NCI-H292 lung cancer cells. Cytotoxicity of each compound obtained from the crude extract of the fungus was determined using SRB assay following standard protocols. 25ppm.72ppm. Thus. All seaweeds showed a significant (p<0. SR Samarakoon2. Sequence was deposited in the GenBank (MF773655). University of Kelaniya. This preliminary investigation revealed that these selected seaweed species could be potential candidates for cancer therapy. apoptosis induced cell death was observed. As the preliminary screening. RN Attanayake3. University of Kelaniya. Sulforhodamine B (SRB) assay. 100ppm and 200ppm concentrations. Furthermore. it is imperative to search for anti-cancer drugs in the treatment of cancers. further studies are highly warranted on the anti- cancer properties of these seaweed species Keywords: seaweeds. giving promising results to find new anticancer drug and further studies on structure elucidation is ongoing. AO/EB staining showed that all three extracts resulted in a significant apoptosis induction in HeLa cells at 24 and 48 hours and apoptotic bodies were also observed after 48 hours but these changes were not observed in primary cells. 50ppm. Preparations of endolichenic fungi (ELF) extracts are commonly used to search for anticancer activity. The growth inhibition rate was compared with controls (treated only with distilled water). anti-cancer. to provide a quantitative analysis of viable cells in a culture was used to screen the efficacy of the crude extract at12. Therefore.5ppm. Fungal strain had 99% similarity to the Xylaria psidii in the GenBank sequences and based on morphology and sequence data it was identified as Xylaria psidii. United States Cancer is a dreadful disease affecting both developed and developing countries and is now one of the leading causes of deaths. Keywords: cytotoxicity. IC50 values of crude extract was 34. Sri Lanka 4 Field Museum of Natural History. This data suggests that tested aqueous seaweed extracts exhibited cytotoxic effects on HeLa cells. apoptosis. Molecular Biology and Biotechnology. University of Colombo. Sri Lanka 3 Department of Botany. In this study. One compound showed the highest anticancer activity compared to the other compounds.cells treated with seaweed extracts (3 mg/ml) after 24 and 48 hours. Secondary metabolites of isolate were extracted with ethylacetate. DNA-intercalate fluorescent dyes. Acridine Orange and Ethidium Bromide (AO/EB) were used to assess the percentage of apoptotic cells in targeted 100 cells under fluorescence microscope. Chicago. the ELF was isolated and molecular identification was carried out following standard procedure. Xylaria psidii 115 . cytotoxicity. G Weerakoon4. Sri Lanka 2 Institute of Biochemistry. SRB assay. Illinois. HAK Maduranga1. Survival percentage of the cells was calculated. Chloroform and methanol fractions of the crude extract (100ppm and 200ppm) obtained by partition were positive for the SRB assay.05) positive effect on proliferation of primary cells and growth inhibition on HeLa cells after 48 hours. Compounds were isolated using silica gel column chromatography and preparative thin layer chromatography (PTLC). in-vitro OP 09-26: Screening anticancer activity of a selected endolichenic fungus (Xylaria psidii) and isolation of active compounds for NCI-H292 lung cancer cells S Sinthujah1. Keywords: A. catappa.1 %) and bio film formation (73. Sri Lanka 3 Department of Food Science and Technology. The Open University of Sri Lanka. P Soysa4. Sri Lanka 3 Department of Obstetrics and Gynaecology. Sri Lanka 2 Department of Agricultural and Plantation Engineering. University of Colombo. Sri Lanka 4 Department of Molecular Biology and Biochemistry. Synergistic antibacterial activity was assessed using macro broth dilution and well diffusion method. Faculty of Medicine. Further. malaccensis. malaccensis showed inhibition of biofilm cell attachment by 98 %. SEM observations showed morphological deformities. Sri Lanka Cross-contamination of bacterial biofilms creates many problems in the health and food industry. Sri Lanka 5 Dermatology unit.5 %) of S. P Udagama1 1 Department of Zoology and Environment Sciences. Therefore. aureus. U Samaratunga2. Also the emergence of multi drug resistant bacteria has led to the discovery of alternatives using natural biomolecules. anti-biofilm activity of Alpinia malaccensis and Terminalia catappa T Somarathna1. Faculty of Medicine. cell aggregations and cell disruptions at 20 mg/ml A. University of Colombo. antibacterial and antibiofilm activity OP 09-28: Differential proliferative activity of selected herbal distillates on human mesenchymal stem cells and hair follicle derived cells P Ratnayake1. malaccensis (2. Sri Lanka Synthetic and semi-biological stimulants are used to proliferate and differentiate different cell types for a range of cellular therapies. Sri Lanka and Department of Food Science and Technology. aureus at 48 h in plantonic forms where > 2 log reduction was observed than if they were used alone. University of Colombo. the aim of this study was to investigate in vitro synergistic antibacterial and antibiofilm activity of crude plant extract of Alpinia malaccensis and Terminalia catappa. antibiofilm activity was confirmed using Scanning Electron Microscopy. catappa (20 mg/ml) showed significant (P<0. catappa and 5 mg/ ml of A. Lady Ridgeway Hospital for Children. In contrast.OP 09-27: Synergistic anti-bacterial.5 %). V Udalamaththa1. aureus. J Senevirathne5. Sri Lanka 2 Department of Ayurveda Basic Principals. Staphylococus aureus 113 was used to evaluate synergistic antibacterial activity and anti- biofilm activity of A. Faculty of Engineering Technology. Faculty of Science. S Wijeratne3. KKDS Ranaweera3 1 Department of Agricultural and Plantation Engineering. A. malaccensis and T. University of Sri Jayewardenepura. aureus. The Open University of Sri Lanka. catappa showed strong antibacterial and antibiofilm activity.5 mg/ml) and T. T. malaccensis and T. malaccensis.05) synergistic antibacterial activity against tested S. Faculty of Graduate Studies. malaccensis at concentration 20 mg/ml inhibited cell attachment (98. University of Sri Jayewardenepura. yet side effects and toxicity of the repeated dosages used and 116 . growth (81. combination of 20 mg/ml of T. catappa. NS Weerakkody2. S. The combination of A. Wickramarachchi Ayurveda Institute University of Kelaniya. Faculty of Engineering Technology. Antibiofilm activity was determined by 96 well microtiter-plate techniques with crystal violet staining. growth inhibition by 91% and biofilm formation inhibition by 69% against S. Faculty of Graduate Studies. Crude plant extract of A. Dried leaves and bulbs of Hippeastrum puniceum and dried leaves of Crinum asiaticum were extracted into methanol and dichloromethane separately. Phloem sap of C. GV demonstrated significantly higher hHFC proliferative activity at 5% compared to both control and positive control (bFGF) (p<0.unavailability of these in developing countries due to exorbitant pricing strongly warrants the search for natural alternatives. This study investigated the proliferative activity ofFicus benghlalensis(FB) and Gymnema/Vernoina (GV) herbal distillates on primary cell cultures of cord tissue derived in house established human mesenchymal stem cells (hMSCs) and human hair follicle derived cells (hHFCs).05). the global concern is to develop natural thrombolytic agents. The thrombolytic activity of plant extracts and sap was performed by an in vitro thrombolytic model where streptokinase and phosphate buffered saline were used as positive control and negative control respectively. In this study. OD values at 540nm.05). The phloem sap of C.asiaticum plant extracts used possess remarkable thrombolytic activity. hair follicle derived cells. Conversely. Compared to untreated cells (control). Sri Lanka Formation of thrombi inside the blood vessels leads to infarction of vital organs which can cause death and is a serious medical problem worldwide. Both FB and GV distillates were prepared according to existing Ayurvedic literature.129) while the other extracts showed moderately higher percentage of clot lysis compared to streptokinase 61.4% (p<0. It is recommended to observe in vivo clot lysis potential and to isolate the natural components of these extracts. NDD Silva1. University of Ruhuna. The aim of the present study is to investigate the in vitro thrombolytic activity of Hippeastrum puniceum and Crinum asiaticum.188% (± 2. while no significant difference was observed on hHFCs at all tested concentrations (1.asiaticum exhibited a promising clot lysis percentage of 49.05). hMSCs were functionally characterized and genetic stability was confirmed by karyotyping. which was not evident on hMSC proliferation (p>0. obtained by a microplate reader were used to statistically analyze the results using Kruskal Wallis test in SPSS version 20. MTT assay investigated the proliferative activity of different FB and GV concentrations on hMSCs and hHFCs. LM Hettihewa2 1 Pharmacy programme. Sri Lanka 2 Department of Pharmacology. Since the available thrombolytic agents such as streptokinase have severe adverse effects.25%-10%)(p>0. asiaticum was collected aseptically in to a flask on ice-salt mixture.169). Keywords: mesenchymal stem cells.05) .878% (± 3. clot lysis. it is concluded that the C. Crinum asiaticum. hMSCs and hHFCs were isolated using explant method.370) and phosphate buffered saline 11. FB demonstrated significantly higher hMSC proliferative activity at 0. University of Ruhuna. Hippeastrum puniceum 117 . Faculty of Medicine. herbal distillates OP 09-29: A comparative study of in-vitro thrombolytic activity of the plant extracts from Hippeastrum puniceum (Wal lunu) and Crinum asiaticum (Tholabo) SK Hettihewa1. Keywords: streptokinase.0. This prototype study suggests that selected herbal extracts at specific concentrations may be used as potential stimulants for hMSC and hHFC proliferation by further validation and standardization of activity guided fractions of the tested herbal distillates. The crude extracts were concentrated using rotary evaporator followed by preparation of dilution series of crude extracts. Faculty of Medicine. thrombolytic activity.39% (± 4. Bergenin. Medications applied on the head are intended to target delivery on tissues underneath. Sri Lanka Unique treatment modalities have been used as head therapies in the Indigenous System of Medicine (IMS) for centuries to treat a wide spectrum of diseases. Thumb circular massage technique was performed for 15 minutes. K-RAS was labeled undruggable due its extremely higher affinity to the natural substrate GTP. Bergenin is a C-glycoside of 4-O-methyl Gallic Acid is isolated from different tropical medicinal plants. Dynamic behavior of the K-RAS G12C mutant protein was studied using a 200 ns long Molecular Dynamics Simulation. It is predicted that binding of Bergenin to K-RAS will destabilize the K-RAS-GTP complex and allosterically affect the binding of GTP to K-RAS. The binding location of Bergenin is indicative of inhibition of the binding of B-RAF to K-RAS which will in return shut down the cancer promoting signal transduction. short subjective instrument was used to assess the severity. In this study. recent studies show that small molecule inhibitors can be developed to disable K-RAS in non-conventional ways. Results show a significant clinical improvement in reducing the severity of insomnia status among patients (p<0. It was found that Bergenin binds to an allosteric binding site which is closer to the natural substrate binding pocket with a high binding energy. this study shows the possibility of using Bergenin as a scaffold to develop a potential allosteric modifier to inhibit the aggressive cancerous activity of K-RAS G12C mutant protein. Nutrition and Indigenous Medicine. Ministry of Health. Various kinds of medicated oils and carrier base oils are used in different head therapies. RS Jayakody Department of Chemistry. ranging from headache to severe nervous system disorders. Since potent and effective Ayurvedic sedatives are not available in IMS. Forty per cent of patients improved from 118 . it was attempted investigate the possibilities of using Bergenin to design novel K- RAS inhibitors. Different parts of these plants are commonly used for treating cancer and many other diseases in the traditional systems of medicine.05). especially in the early days after admission to the wards. However. These therapies also range from therapeutic massage (Śiroabhyaṅga) to oil dripping (Śirodhārā).OP 09-31: Bergenin. cancer. Keywords: K-ras G12C mutant protein. The mean and range of participants’ ages were 47 – 67 years and all were males. 15 patients were selected for the study. computational study OP 09-32: Trans-cranial route of drug delivery in ancient wisdom of Ayurveda: A case study V Seneviratne Ayurveda Research Institute. Therefore. The insomnia severity index (ISI). University of Sri Jayewardenepura. a potent natural scaffold for designing K-RAS G12C mutant protein inhibitors in the cancer treatments as revealed by computational studies CR Herath. Insomnia is one of the most common sleep disorders world-wide and is a major problem of indoor patients. A volume of 15 ml oil was applied on scalp over three vital points twice a day. Authentic indigenous oil was used as the test drug in this study. 40% of colon cancers and 20% of non–small cell lung cancers. Bergenin was docked in to the relaxed KRG12C using automated molecular docking. which is detected in 90% of pancreatic cancers. Sri Lanka Point mutation G12C of K-RAS gene is the most frequent mutation of K-RAS. we encounter difficulties in managing such patients. W. proximate composition.71±0.93±0.35±2.77±2. Chonnam National University.01 & 3.77±0. Keywords: marine snail. In meat. Chorusspp (chomsora) may have a potential to become a very interesting source for some proteins and flavor peptide development. Yeosu.10±0.88 (Alcalase) & 92. Carbohydrate % and phenol contents % among enzymatic hydrolysates were 3. This study highlighted and provided new information on the proximate composition and antioxidant potential of different body parts of Chorus spp (chomsora). 5. 100 °C) and 4.00(H.85(Alcalase) respectively. Hence. chorus spp (chomsora). Republic of Korea Chorus spp (chomsora) is a traditionally consumed gastropoda marine snail in east asia.66. insomnia. and % lipid content of 2. flavourzyme recorded highest % DPPH radical scavenging activity & % ABTS radical scavenging activity respectively. Carbohydrate % and phenol contents % among enzymatic hydrolysates were 52. Faculty of Medicine University of Sri Jayewardenepura.04±0.severe level to sub-threshold insomnia. It is known for good heath and increasing nutritional and economic importance. there is limited information on their nutritional composition. Department of Anatomy. 28 °C. and the edible parts of Chorus spp (chomsora) may be utilized for special dietary applications requiring high amounts of amino acids. the highest protein %.03(pepsin) . Sri Lanka 2 Department of Marine Bio-Food Sciences.30±0. where significantly high concentrations of carbohydrates and phenolic compounds were present. % protein content of 40.65±1.79±0.70 (Pepsin) in meat and visceral mass respectively. 100 °C) and 10.68. However.60 (Trypsin) .63 in meat and visceral mass respectively.15±0. Keywords: Centella asciatica.70 & 52.93±0.93±1. insomnia severity index OP 09-33: Proximate composition and potential antioxidant capacity of enzymatic extract from marine snail Chorus spp (chomsora) meat and visceral mass RMS Gunathilaka1. Trypsin and Pepsin where in visceral mass hydrolysates RT. ABTS+ 119 . DPPH. it can be concluded that the treatment used to treat insomnia was effective and could be developed as a novelty route of drug delivering. A significantly higher concentration of nutritional elements such as fat and proteins were found in the visceral mass than those in the meat.19 (Protomex) respectively. Chorus spp (chomsora) contained a % moisture content of 2.18 & 29. As proximate analysis showed.38(H. the highest protein %. In visceral mass. 15.W.W. The highest estimated yield recorded was 87. Among meat hydrolysates. 47 per cent severe level to moderate severity insomnia within 3-5 days duration and 13 per cent showed no improvement.43±0. G Ahn2 1 Interdisciplinary center for innovation of Biotechnology & Neuroscience Genetic diagnostic & Research Laboratory. The edible tissues including meat and visceral mass were valuable sources of proteins and antioxidants. The TLC for all three calli samples (Rf values 0.42 mg Trolox equivalents/g of extract and 198. leaf discs. Sri Lanka 4 Department of Chemistry. University of Kelaniya. LDAM Arawwawala3. The results from the 120 .31) gave the same results. showing the presence of terpenoids. University of Kelaniya. RPVJ Rajapakse2 1 Department of Kaumarabruthya & Stree Roga. Sri Lanka Munronia pinnata (Binkohomba) is a valuable medicinal plant that is being widely used in Ayurveda and which possesses a variety of medicinal properties. Therefore herbal compounds with good anti-oxidant properties are of great demand in the health sector. they can represent a source of new compounds with antioxidant activity. 0.65 ± 4. University of Colombo. GC-MS and some preliminary phytochemical tests. 103. free radicals OP 09-35: Identification of the secondary metabolites present in the calli of Munronia pinnata NS Hapuarachchi1. Keywords: ayurveda.11 ± 17. According to these results it can be concluded that MMYACH choorna is rich in antioxidant properties. SD Hapuarachchi2.2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) and diammonium salt] of hot water extract of MMYACH choorna using in vitro antioxidant assays. Fifty grams of choorna was taken into a round bottom flask and 150 ml of distilled water was added and refluxed for 3 h. The objective of this study was to extract and identify some major secondary metabolites found in calli of Munronia pinnata. Sri Lanka Experimental evidence suggests that free radicals can be involved in a high number of diseases. Sri Lanka 2 Department of Veterinary Pathobiology. Sri Lnaka 3 Industrial Technology Institute. ORAC: oxygen radical absorbance capacity.55 mg Trolox equivalents/g of extract respectively. Calli obtained from the explants. Freeze dried extract was subjected to antioxidant assays. Gampaha Wickramarachchi Ayurveda Institute. including inflammation. DPPH: 1. 481. WMD Wickramaarachchi2. This plant is being overexploited due to its high demand. the composition of which cannot be revealed yet due to application of patent rights. MMYACH choorna is a novel ayurvedic formulation of 9 Sri Lankan medicinal plants tested for usage as an anti-inflammatory drug. the extract was filtered and the filtrate was then concentrated and freeze dried. As plants produce a lot of antioxidants to control the oxidative stress caused by sunbeams and oxygen. therefore micro propagation is being practiced as a solution. Faculty of Science.20 ± 4. antioxidant properties according to ORAC. Sri Lanka 2 Institute of Indigenous Medicine. WTPSK Senerath1 1 University of Sri Jayewardenepura. petals and immature seeds via in vitro propagation were extracted into methanol and water. antioxidants. 79.94 mg gallic acid equivalents/g extract. These calli samples were subjected to TLC.OP 09-34: Evaluation of the potential antioxidant properties of MMYACH choorna: A novel ayurvedic poly-herbal formulation HP Wakkumbura1. HPTLC.50 ± 4. An attempt was taken to investigate the antioxidant properties [in terms of TPC: total polyphenolic content.30 mg Trolox equivalents/g of extract. DPPH and ABTS of MMYACH choorna were. Results revealed that the total polyphenolic content.1-diphenyl-2- picryl-hydrazyl and ABTS: 2.15. University of Peradeniya. Afterwards. Faculty of Veterinary Medicine and Animal Science. JA Liyanage4. Trincomalee. Thereby. protective reflex OP 09-37: A potent antibacterial agent (-)-hardwickii acid from the leaves of Croton oblongifolius Roxb K Wijesekera Faculty of Medicine. as a purgative. Optimization of the culture medium conditions and extraction methods could increase the amount of the chemical compounds extracted by the calli. The methanol extracts of petal and seed calli samples were positive for steroids. This Hercough syrup contains Glycyrrhiza glabra Linn. Sri Lanka Cough is a protective reflex action that clears large breathing passages from fluid. According to the duration. GC-MS. COR is a rich source of diterpenes which 121 . Collected data were statistically analyzed. Moreover. Pipper longum Linn.sublyratus. University of Ruhuna. TLC OP 09-36: Effect of Hercough syrup in the management of chronic cough.A case series KGP Wijesekara1. Sri Lanka Croton oblongifolius Roxb (COR) has been used as a traditional medicine for dysmenorrhea. steroids. After obtaining a detailed history and complete physical examination they were informed regarding the aim of the present study and action of the drug. If any kind of cough lasts more than eight weeksit can be considered as chronic cough. Patients were also examined one month after the treatment as a follow up period. foreign particles and microbes. Gampaha Wickramarachchi Ayurveda Institute. pinnata. University of Kelaniya.preliminary chemical tests showed that aqueous extracts of all three calli samples were positive for flavonoids and the leaf and seed calli were positive for tannins. Keywords: chronic cough. Hercough syrup was found to be effective for the management of chronic cough. Thirty patients who were diagnosed with chronic cough were selected from Colombo. in conjunction with C. GUA Kumara2 1 Gampaha Wickramarachchi Ayurveda Institute. cough syrup. Jaffna. Keywords: M. HPTLC. irritants. Zingiber officinale Linn. and to treat dyspepsia and dysentery. Badulla and Galle districts. pinnata such as terpenoids. University of Kelaniya. Adhatoda vasica Linn. Then all patients were treated with 15ml of Hercough syrup two times a day for the period of fourteen days continuously. calli. sub-acute and chronic. cough can be classified as acute. According to the result of data analysis. The GC-MS analysis gave the chemical compounds present in the samples of leaf and petal calli as well as the mother plant. The purpose of this study was to study the effect of Hercough syrup in the management of chronic cough. Assessment was done before treatment. during the trial and after treatment. it was concluded that major secondary metabolites present in M. Sri Lanka 2 Kaumarabhritya and Striroga. it has been used to treat gastric ulcers and gastric cancers. Solanum virginianum Linn. An HPTLC fingerprint of the methanol extracts of all three samples and that of the mother plant was also obtained. Solunum melongena Linn and Curcuma longa Linn. tannins and flavonoids could be extracted at the callus stage. 15μg/mL. Leaves of C. Panjwani Center for Molecular Medicine and Drug Research.45 g/mL). it showed potent antibacterial activity against Bacillus cereus with a minimum inhibitory concentration (MIC) of 1. Infectious and non-communicable diseases are emerging and are responsible for millions of deaths worldwide.760. dose-dependent COX-2 inhibition (IC50:12. hepatoprotective traditional healer HDSM Perera1.730. MI Choudhary4 1 Industrial Technology Institute. oxidative burst (OB) on human whole blood (WB) and isolated polymorpho-nuclear neutrophils (PMNs). ELE showed promising. Oxidative stress and inflammation are strongly associated with liver diseases. In the case of infectious diseases. University of Karachi. the development of drug resistant microbes has created a dangerous situation.5 mg TE/g) and ORAC (942. Thailand and powdered. Pakistan 4 H.36 g/mL).33 g/mL). respectively.89 g/mL. Chromatographic separations have led to the isolation of (-)-hardwickii acid and was inactive for cytotoxic assays. traditionally used to treat liver diseases.500. Pakistan Osbeckia octandra is an endemic Sri Lankan medicinal plant.21 %. E. oxygen radical absorbance capacity (ORAC). Biological activities including cytotoxicity and antibacterial activities were evaluated with standard procedures. Ibuprofen IC50:5. MIC OP 09-38: Studies on anti-inflammatory and anti-oxidant potential of Osbeckia octandra: An endemic.950. cycloxygenase 2 (COX-2).52.02 g/mL) and PMNs (IC50:4. A Jabeen3.120. University of Colombo. indicating significant inhibitory effect on arachidonic acid cascade. FRAP (3607. the need of new drugs with better curing capacities is clear and the current research uses natural products as an essential component in the search for new therapeutic agents. ELE possessed remarkable anti-oxidant potential with high DPPH free radical scavenging activity (IC50:7. OVDSJ Weerasena2.52 TE/g) attributed to high 122 .120. Keywords: cytotoxicity. oblongifolius were collected from Nakhonsawan province.220. arachidonate-5- lipoxygenase. University of Karachi. Panjwani Center for Molecular Medicine and Drug Research. air-dried leaves were macerated with dichloromethane to yield a crude extract which was subjected to several chromatographic techniques. inducible nitric oxide (iNO) production. octandra. The present study focused on investigating the anti-inflammatory and anti-oxidative effect of O. ferric reducing anti-oxidant power (FRAP).50 g/mL) and A5-LOX inhibition (IC50:24. (-)-hardwickii acid is a potential candidate to treat infectious diseases. Research Institute of Chemistry. The suppression of NO production was found to be low at 500 g/mL (6. prominently associated with liver diseases namely.25 and 3.possesses various biological activities. Therefore. International Center for Chemical and Biological Sciences. J. ELE exhibited a strong OB inhibition on WB (IC50:23. diterpenes. R Samarasekera1. Sri Lanka 3 Dr. total polyphenol content (TPC) and total flavonoid content (TFC). Molecular Biology and Biotechnology. International Center for Chemical and Biological Sciences. S Handunnetti2. However. and Dr. Structure of the compound isolated was established with the aids of spectroscopic data. Ethanolic leaf extract (ELE) was used to study the inhibition of selected inflammatory mechanisms. International Center for Chemical and Biological Sciences.869. Anti-oxidant potential was determined with the means of DPPH free radical scavenging. Cell viability: > 85 %).56μg/mL with the standard drugs tetracycline and vancomycin having MICs of 6. Sri Lanka 2 Institute of Biochemistry. The results were statistically analysed. L Turyanska3.001).18 mg GAE) and TFC (32.50 QE/g). thus supporting traditional use. in-vitro experiments OP 09-40: Saraca asoca as a potential inhibitor of glycation and glycation induced cross-linking O Dheerasekera1. University of Nottingham. Thus we tested the anticancer effect of leaves and stems of this species. Sri Lanka and Center for Biomolecular Sciences. Finely powdered dried leaves and stems were subjected to ethanol extractions. However. cell cycle. Sri Lanka. the stem extract was not active. Sri Lanka. The results were significant at p<0. especially HER2+ SKBR3 cells by MTT assay (GI50= 17. affecting the structure and function of 123 . TD Bradshaw2 1 Interdisciplinary Center for Innovations in Biotechnology & Neurosciences. breast cancer. cell migration and reactive oxygen species (ROS) were used to determine anticancer activity. United Kingdom Cancer remains a global burden. Sri Lanka 2 Department of Biochemistry. Some of the AGEs lead to intermolecular cross-linking.0001).05. This family is found to possess anticancer activity. we have found that P. MTT. United Kingdom 3 Faculty of Science. United Kingdom 2 Centre for Biomolecular Sciences.45 µg/ml ± 2. thus further work is warranted to isolate chemical compounds from this plant species. The study demonstrates anti-inflammatory and anti-oxidant potential of ELE of O. apoptosis. University of Peradeniya.352. octandra. The leaf extract was able to block the G1 phase (84%) compared to control (75%) (p<0. Keywords: Cycloxygenase-2. University of Nottingham. A significant apoptotic population was also observed in cells that were treated with the leaf extract (7%) compared to control (3%) (p<0.0001). Leaves and stems of P. nitric oxide. University of Peradeniya. Advanced glycation end-products (AGEs) formed as a result of non-enzymatic glycation are key mediators of the pathogenesis of chronic diabetic complications and acceleration of skin ageing.650. Ethical approval was obtained from the Department of Forestry. Plumeriarubra is commonly found in Sri Lanka and this plant belongs to the Apocynaceae family. Sri Lanka. Thus. HKI Perera2 1 Postgraduate Institute of Science.TPC (237. It was found that 2x GI50 of the leaf extract completely inhibited colony formation in clonogenic assay (p<0. further investigations were carried using P. clonogenic assay. The leaves could be promoted as a natural health enhancing food based on promising bio-active properties observed in the present study. M Al-Hayali2. anti-oxidant OP 09-39: A study on the potential anticancer activity of Plumeria rubra AI Kuruppu1. University of Nottingham. rubra leaves possess some anti-cancer activity. The leaf extract inhibited the growth of breast cancer cell lines. gamma H2AX. oxidative burst. Faculty of Medicine. Keywords: natural products. In conclusion. University of Sri Jayawardenepura. L Zhang2.05) and it further generated high levels of ROS in cells (p<0. Faculty of Medical Sciences. rubra leaf extract.0001). This agent was able to cause a minor amount of DNA double strand breaks by gamma H2AX analysis (p<0. Arachidonate-5-lipoxygenase. The discovery of novel anticancer agents from natural sources remains pivotal.05). rubra were collected from Colombo. Maximum inhibitory effects were observed in SA seed extract. glycation. Glycated BSA showed an increase in migration.05 mg/mL. In conclusion. The objective of this study was to investigate the inhibitory effects of Saraca asoca (SA) extracts on glycation and glycation induced cross-linking. bark and leaves of SA were prepared. Appropriate controls and the standard inhibitor aminoguanidine (1mg/mL) were used. Aliquots of BSA incubations were analyzed using native polyacrylamide gel electrophoresis (PAGE) and those of lysozyme incubations were analyzed using sodium dodecyl polyacrylamide gel electrophoresis (SDS-PAGE). bark and leaf extracts showed potential inhibitory effects on glycation and glycation induced protein cross-linking. All three SA extracts showed inhibitory effects on glycation and glycation induced protein cross-linking at 1 and 0. inhibitory effects of bark and leaf extracts were reduced while the effect of seed extract remained.1 mg/mL) of extracts. Cross-linked products of lysozyme were observed as high molecular bands.05 .5 M fructose at 37°C and pH 7. SA seed. in the presence or absence of different concentrations (0. Bovine serum albumin (BSA) and lysozyme were incubated with 0. cross-linking 124 . Keywords: Saraca asoca. Methanol extracts of seed.4 for 21 days.1 mg/mL. At 0.affected molecules. propagation and conservation of medicinal plants Abstracts of Keynote Speakers and Oral Presenters 125 . cultivation. Track 10 Organic farming. Developing markets for herbal products. Medicinal Plants in India: Diversity. Distribution and their Conservation Approaches Dr. Habitat degradation. and over harvesting. Bangalore – 560065 India. Medicinal plants are one of the most sensitive commodity areas of research exhibiting diverse life forms and occurring under varied ecologies practically occupying all floristic regions of the world today. can trigger a demand that cannot be met by available or legal supplies and demands a conservation initiative. programs to support in situ conservation of species both within and outside conservation areas. Velusamy Sundaresan CSIR . A range of strategies have been employed in recent decades to build upon this interest and address conservation issues associated with medicinal plants. Herbal products. and cryopreservation and conducting ethno medical survey to explore utilization of medicinal plants. Conscious efforts to conserve and protect global biodiversity are a concerned phenomenon. Keywords: Medicinal plants. All cultures from ancient times to the present day have used plants as a source of medicines. India is endowed with rich and diverse forest resources among 12 mega-biodiversity countries of the world and Western Ghats stands as a one of the richest biodiversityhotspots. particularly those that are harvested from the wild.The most serious proximate threats when extracting medicinal plants generally are habitat loss. ex-situ techniques involving seed banks. These include international and national policy processes. habitat degradation. over harvesting 126 .Central Institute of Medicinal and Aromatic Plants. gene banks. Research Centre. Conservation of medicinal plants in its biocultural perspective not only implies conservation of biodiversity but also places an equal emphasis on conservation of cultural diversity. The overall conclusion of the presentation is to initiate and support for conservation management and to promote in- situ and ex-situ conservation of medicinal plants in and around site of global significance. The Taxonomic Rank of a plant starts from the Kingdom. with Cassia auriculata Linn. This knowledge is sometimes hidden in legends and associated with interesting stories. which may be selected to undergo research. known as Ranawara. by villagers or indigenous physicians who collect and prescribe drugs for treating their patients. which he called Binomial Nomenclature. Therefore. Correct Botanical Identification of any medicinal plant. should confirm whether the species is common or rare. but more than one nameare locally engaged for a single plant. for ranking a given plant up to the Family. But different species can be identified according to the arrangement of stamens. various morphological characters can be used to separate out the botanical identity. Species followed by the Author or Authors who are involved with naming or renaming the plant after scientific investigations. Genus. album the Species and Linn. Provincial Ministry of Health and Indigenous Medicine. Swetha Chandana or White Sandalwood can be written as Santalum album Linn. Present methodology that we follow to write a Botanical Name was introduced by the great Botanist Linnaeus during the year 1750. Uva Province. Family. Plant taxonomy is nothing but the subject called Systematic Botany that is involved with scientific techniques to search morphological characters carefully. Morphological characters of various plants were carefully observed by grandparents and the traditional knowledge that they learnt from nature passed to the next generation without keeping any secrets. the scientific name of Suduhandun. Here in this name Santalum is the Genus. which are used for Traditional Systems of Medicine in Sri Lanka. Medicinal Plants Conservation and Cultivation Program. a name includes the Genus. Advisor. Genus and finally ends with Species name. Species and Sub- species etc. In this method. Prof. Comparing Cassia fistula Linn. then the Division. medicinal plants 127 . Keywords: Taxonomical Identification. indicates the author’s name in short form. Sub Divisions up to Sub Species and even variety level. for the benefit of global health. Further those can be dived into Sub Kingdoms. Fixation of the accepted botanical name for a plant can be done through its Taxonomical Identification. Provincial Department of Ayurveda. Tamil and Sinhalese names which are popularly applied for these herbs. [Ehala]. Sri Lanka Medicinal Plants. Taxonomical Identification of flora is not a new subject but coming from our generations. Class. Piyal Marasinghe Associate Professor. have different local names in different regions of the Island. Order. For example. Importance of Taxonomical Identification Associated with Herbs used in Indigenous Systems of Medicines in Sri Lanka Assoc. Indigenous Systems of Medicines. belongs to the family Cucurbitaceae and are ascribed to possess anti-diabetic effects in the traditional systems of medicines. information on proximate nutrients composition and biological activities of domesticated and non- domesticated populations of M. HKMS Kumarasinghe2 1 Grain Legume and Oil Crops Research and Development Centre. Therefore. commonly known as bitter gourd. Keywords: Cucurbitaceae. The mineral composition revealed that Ca was the major mineral in both fruit and leaves of all populations followed by Na. In Sri Lanka. reticulata habitats. reticulata plants at the experimental site were pruned to the same height (90 cm from ground level). Therefore. minerals. GA Prathapsinghe1. crude fat and crude protein. Matale green. University of Ruhuna. University of Ruhuna. the present study was undertaken to determine mineral content and physiochemical properties of fruits and leaf extracts of six populations of domesticated and non-domesticated Momordica charantia. Sri Lanka 3 University of Peradeniya. all S. Sri Lanka Momordica charantia. The investigation was carried out at the medicinal plant garden at Gamudawa. charantia cultivated in Sri Lanka are scarce. RM Dharmadasa2. and Cu respectively.OP 10-01: Proximate and nutrient analysis of domesticated and non-domesticated Momordica charantia populations grown in Sri Lanka KKS Withanage1. Before starting the experiment. Zn. Getakaravila and Small population. Macro-elements were analyzed using Atomic Absorption Spectrophotometer. The planting material collections have to be done very carefully with full awareness of the medicinal value of the plant as well as with knowledge of S. Sri Lanka 2 Faculty of Agriculture. S Subasinghe2. Faculty of Agriculture. Fe. this study about pruning frequency is essential to the sustainable harvesting of S. reticulata for commercial cultivation. KKIU Arunakumara2. Comparatively the higher amounts of nutrients were observed in leaf extracts compared to the fruits. Mg. crude fiber. major Thinnaweli white. Sri Lanka. reticulata is included in the IUCN red list because of over harvesting. reticulata is widely distributed within Asian countries. The results demonstrated that all domesticated and non-domesticated M. Momordica populations. the ecological conditions of rain forests as well as near water reservoirs in dry parts of the country provide facilities for survival in nature. However. MKTK Amarasinghe2. Momordicacharantia 43. LJAPA Jayasooriya3 1 Wayamba University of Sri Lanka 2 Industrial Technology Institute. Replicates were selected for each treatment based on the 128 . Department of Agriculture. S. charantia populations tested contained marked amounts of major and macro minerals. unsustainable harvesting and lack of care to their habitats when collecting plants from the wild. S. DC Abeysinghe1. Sri Lanka The medicinal plant Kothalahimbatu (Salacia reticulata) plays an incomparable role with other medicinal plants in the Ayurvedic medicinal system. Unfortunately. Kalukaravila. Our findings clearly demonstrate the suitability of the leaf as a major nutrient rich source for the nutraceutical industry. proximate analysis OP 10-02: Determination of best pruning frequency for maximum dry leaves and stems yield of Salacia reticulate EKEC Nayana1. anti-diabetic. charantia seeds harvested at mature and ripened stages. Several scientific research studies have revealed. crude fiber. 2 Industrial Technology Institute. charantia contain an array of bioactive molecules and hence seeds possess anticancer. ash. T4 – six months after first pruning and T5 – seven months after first pruning.mean SD values and CV values of previous pruning data.charantia are cultivated in Sri Lanka. seeds harvested at two maturity stages AMGN Rathnayke1. anti-inflammatory and anti-atherosclerotic activities. Even though several domesticated and non-domesticated populations of M. Under physiochemical composition. There was no clear difference in crude fiber among mature and ripened stages. stems OP 10-03: Physiochemical contents of domesticated and non-domesticated populations of Momordica charantia L. moisture. The results revealed that pruning at four and five months intervals would reduce unsustainable harvesting of leaves and stems. Chemical constituents vary according to species or variety. dry matter. S. pruning frequency. RM Dharmadasa2. There was significant difference (p<0. T2 – four months after first pruning. antitumor. crude fat. the present study describes the physiochemical constituents of six domesticated and non-domesticated populations of M. Momordica charantia seeds. most of the chemical constituents were increased and hence seeds from the ripened stage in both domesticated and non-domesticated populations are more suitable for therapeutic properties. Sri Lanka 3 Faculty of Livestock Fisheries and Nutrition. LJAPA Jayasooriya4 1 Faculty of Agriculture and Plantation Management. DC Abeysinghe1. environmental condition and genetic makeup. Wayamba University of Sri Lanka 4 Faculty of Veterinary Medicine and Animal Science. Keywords: leaves. information on bioactive molecules present in seeds at different maturity stages are lacking or are scattered. ash. At the four months pruning interval. Randomized Completely Block Design (RCBD) was used with five replicates. It could be conclude that seeds of all tested Momordica charantia populations harvested at mature and ripened stages showed the presence of physiochemical constituents and when seed developed from a mature stage up to a ripened stage. seeds of M. University of Peradeniya Momordica charantia L is a well known therapeutically important medicinal plant belonging to the family Cucurbitaceae. reticulata. Treatments were T1 – three months after first pruning. crude protein and crude fat content were determined according to the official AOAC methods (1990). T3 – five months after first pruning. respectively. dry matter. anti- mutagenic. physiochemical composition 129 . maturity stage. While maturity progressed. The significantly highest stem dry weights were recorded from five months after pruning. GA Prathapasinghe3. Therefore. the parameter of number of leaves was significantly higher (pr>f) than other treatments. crude protein and dry matter content were increased and moisture content was decreased.05) in moisture. antioxidant. Keywords: Cucurbitaceae. Wayamba University of Sri Lanka. crude fat and crude protein among mature and ripened stages of Momordica seeds. Two pruning cycle data was used for the analysis. 49±2. While carotenoid and mineral content of several morphotypes of C. The morphotypes were named as G-1 (heen / wel). total phenolic content OP 10-05: Phytochemical content and antioxidant capacity of different parts of Tagetes erecta Linn. phosphorous. TPC and TFC were determined using Ferric Reducing 130 . erecta is considered as a multipurpose medicinal plant due to its uses.6 %.10±1. 2 1 Agricultural Biotechnology Center. Although T. T. The green well grown leaves were harvested for analysis and the nitrogen.OP 10-04: Assessment of total phenolic content and antioxidant activity of different Centella asiatica (Gotukola) morphotypes CHWMRB Chandrasekara1. University of Peradeniya. potassium and calcium contents were measured with standard laboratory procedures.02 mg of GAE/100 g while the antioxidant index ranged from 6. Antioxidants and some other secondary metabolites contribute to such medicinal effects. information on presence of secondary metabolites and antioxidant activity of plant parts among different populations are few and scattered. The total phenolic content and antioxidant activity were measured using folin ciocalteu and 2-diphenyl-1-picrylhydrazyl assays respectively. Each assay was duplicated. Different morpotypes possess differences in biochemical traits irrespective of the similarities of environmental and soil conditions. their biochemical properties have not been studied. G-2 (salad/wavy). ranging from ornamental to herbal-medicinal remedies. Further studies are needed for identification of overall performing genotype/s for commercial propagation.91±0. the present study was undertaken to determine the Total Phenolic Content (TPC). Faculty of Agriculture. Wayamba University of Sri Lanka 3 Industrial Technology Institute. Protein content of different morphotypes ranged from 26. Sri Lanka 2 Department of Plant Sciences. the highest antioxidant index was found in G-1. antioxidant activity. (Asteraceae) in different populations PMAS Perera1. Therefore. stems. G-3 (bush). CA. assiatica have been studied previously.02 mg/g and maintained under greenhouse conditions for four weeks. the current study was designed to asses such properties of five different morphotypes when they were grown in the same environmental and soil conditions. phosphorous 100. Davis. Potassium was the major mineral found in all morphotypes with the average of 18.39 ppm and potassium 0. USA Centella asiatica known as “Gotukola” is a green leafy vegetable used in Sri Lankan cuisine as well as ayurvedic and traditional medicine. The total phenolic content ranged from 3.19-12. PCG Bandaranayake1. erecta is a multipurpose plant. Sri Lanka Tagetes erecta Linn. Total Flavonoid Content (TFC) and Total Antioxidant Capacity (TAC) of different parts (flowers. G-4 (medium) and G-5 (giant) and grown in soil consisting of nitrogen 1.84±0. Therefore.6-20. While the highest total phenolic content was reported in G-5. RM Dharmadasa3 1 Wayamba University of Sri Lanka 2 Dean of the Faculty of Agriculture and Plantation Management. leaves and roots) of nine Tagetes erecta populations.05mg/g. Keywords: Centella asiatica.06 mg/g. University of California. DC Abeysinghe2.25-35%. popularly known as “Marigold” belongs to the family Asteraceae and is grown as ornamental plants and thrive in varied agro-climates. The TAC. Peradeniya. medicinal and pharmacological applications depend on continuous supply of quality uniform raw materials. The highest TPC. TFC and TAC were recorded in population 13 (P13). Further. followed by Leaves>Stems>Roots respectively. PCG Bandaranayake2 1 Department of Agricultural Biology.96amg TE/ g DW respectively. Since cinnamon is naturally adapted for cross pollination. genetic diversity. Keywords: antioxidant capacity. Faculty of Agriculture. Since the genetic background and the environmental conditions decide the phenotypic variation of both primary and secondary metabolism. modified Folin-Ciocalteu colourimetric method and colourimetric method respectively. TPC0. only eight resulted in polymorphic bands between parents. Sri Lanka Cinnamomum zeylanicum known as true cinnamon has been used for culinary and medicinal purposes for centuries. TFC.53 ± 2. genetic purity of the initial propagation material will decide both quantity and quality of the yield. Identified polymorphic primers were used to test the genetic diversity of seedlings. the cinnamon based industries have gained more attention than ever. University of Ruhuna. TFC. ISSR. The recent scientific work has shown positive impacts on non-communicable and communicable diseases. 88. Sri Wijaya mother plant and Sri Gamunu mother plant growing next to it. for sustainability of such industries. Among thirty seven ISSR primers tested and resolved on 8% polyacrylamide gels.Antioxidant Power (FRAP) Assay. Keywords: Cinnamomum zeylanicum Blume. and TAC were reported in flowers. Faculty of Agriculture. Tagetes erecta OP 10-06: Genetic Fingerprinting of seedlings from an open pollinated mother plant of Cinnamomum zeylanicum Blume (cinnamon) NMN Liyanage1. identification of genetic diversity created in a single pollination event is important for the reproducibility and traceability of products.66amg GAE /g DW. The significantly higher TPC. Phenolics. The results of the present study are important for determining the suitable harvesting parts of the plant for potential industrial use of Tagetes erecta.04 ± 0.00amg RE/g DW and 446. Sri Wijaya and Sri Gamunu. However. cinnamon 131 .39 ± 1. Vegetatively propagated cinnamon plantation with two varieties. TFC and TAC. University of Peradeniya. Results revealed a considerable genetic diversity among seedlings. All tested parts demonstrated the presence of TPC. Flavonoids. using Inter Simple Sequence Repeat (ISSR) regions. Sri Lanka 2 Agricultural Biotechnology Centre. more than in other parts of the plant. grown in adjacent rows was the study site. and TAC in flowers of P13 were reported as 59. While some are similar to parents. Therefore the present study was carried out to study the genetic diversity of mother plants and a progeny of a recently released variety Sri Wijaya. DNA was extracted from seedlings. Asteraceae. AL Ranawake1. others show different levels of polymorphism. Ten ripened seeds were harvested from a Sri Wijaya plant and germinated. 132 . Track 11 Regulations related to traditional and complementary medical products and education of traditional and complementary medical practices and practitioners 133 . traditional medicines 134 . K. Regional Office for South-East Asia. Sri Lanka was yet unable to build an effective intellectual property rights regime especially for the traditional medical sector. innovation and intellectual property.Honarary Consultant in Ayurveda Community Medicine. P. the Member states focused on traditional medicine and The WHO traditional medicine strategy: 2014–2023. This study was undertaken to explore and suggest possible courses of action most appropriate for member states of the South East Region (SEAR) with particular reference to Sri Lanka. It has lost a number of opportunities in history to use its own traditional medical knowledge for the benefit of future generations due to loss of patentability in traditional knowledge. Consultant Physiscian and Clinical Pharmacologist at National Ayurveda Teaching Hospital. Head of the Department of Ayurveda Pharmacology and Pharmaceutics. Our suggestions and findings can be the possible course of actions for most of the member states of SEAR in IPRs and trade in Traditional Medicines.21 Global strategy on public health. Sri Lanka and Dr. Manisha Shridhar World Health Organization. Sri Lanka. Rajagiriy. Perera Senior Lecturer. University of Colombo. Therefore Sri Lankan traditional sector has to face several difficulties especially in international trade. It should be encouraged to develop innovative patent systems for the pharmaceutical sector. Thus. Keywords: sui generis. there is a need to develop an alternative sui generis system which meets the needs of the holders of traditional knowledge. Institute of Indigenous Medicine. Intellectual Property Rights (IPRs) and Trade in Traditional Medicines Related to South East Aisa Region and Sri Lanka Dr. WHA61. Sri Lanka has already implemented some rules and regulations but these Acts and Ordinances are deals with natural resources which collectively indicate a policy in favour of physical protection of natural resources. (WHO/ SEARO) In the World Health Assembly Resolution. It is important to develop guidelines or laws and enforce them to ensure benefit sharing with the community for commercial use of traditional knowledge. intellectual property rights. but are not specific laws relating to medicinal plants and traditional knowledge. which can prevent invalid patents from being granted. Danister L. This form of protection leads to domestic legislation that requires voluntary or mandatory disclosure of TK that is incorporated into the invention being patented and IPR of TK holders is granted in a mutually beneficial manner. Keywords: IPR. In Sri Lanka traditional medical knowledge TMK conservationproject is a national initiative proposed to establish a digital repository of available TMK sources safeguarded with aIP protection system that can contribute positively for the advancement of the indigenous medical sector. UNESCO Research Scholar. and traditional cultural expressions. Defensive protection involves using measures to prevent IPR from being granted to unauthorized individuals andhas largely focused on creating databases to consider TK during "prior art" searches. TMK. University of Kelaniya Chairman. Ministry of Indigenous Medicine Several international conventions address the subject of IP aspects of traditional knowledge (TK). genetic resources. PGIAR. Positive protection is referred to as “sui-generis” measures for protecting TK which involves TK holders directly acquiring IPR using patents or alternate forms of protection. National Expert Committee on Traditional Knowledge Former Member. National Intellectual Property Advisory Commission Former Consultant. MPCSU Project. TKDL to protect the misappropriation of TK in India became the first national initiative to create a database. TKDL 135 . TKDL largely depends on documenting TK available as public domain in existing literature and converting it into digitally accessible pages. while the WIPO IGC initiated to develop an international legal instrument to ensure the effective protection of TK. Protection of Traditional Medical Knowledge from Misappropriation Dr. In 2001. This project mainly focuses on strengthening local traditions and facilitating sustainable transmission through an effective documentation process and defensive system based on mutually agreed terms and conditions that can prevent misappropriations. As an initiative TMKproject envisages to establish a digital library on published TMK through printed media and medical palm-leaf manuscripts deposited in state owned libraries. and UNESCO convention on ICH. ITPGR. including the CBD. Perera ICH Expert. Siddha. effective transfer of knowledge through proactive education. Traditional systems of Medicines are at an exciting stage in its development as a health care system. duration and degrees of Under graduate as well as postgraduate in Ayurveda are regulated by Minimum Standards in Education Regulations. Siddha and Homoeopathy. India Government of India Growth of any knowledge base depends on a dynamic continuum of a tripod of the creation of new knowledge through research. In India. Central Council of Indian Medicine also recognizes the Degrees in Ayurveda awarded by Universities or Board outside India and includes in II Schedule of Indian Medicine Central Council Act 1970. Central Registration Regulations and Professional Conduct & Code of Ethics regulate Registration and practice of practitioners respectively. eligibility. Under Graduate Degrees in Ayurveda from Countries of Nepal and Srilanka are recognized under Central Council of Indian Medicine. Unani. Standards of Institutions imparting education in Ayurveda are regulated by notified Minimum Standard Requirements and Infrastructure Facilities Regulations and amended from time to time. Siddha and Homoeopathy (AYUSH) in 2003. Central Council of Indian Medicine. Homoeopathy 136 . Yoga. Keywords: Ayurveda. The admission process. Naturopathy. with focused attention for development of Education and Research in Ayurveda. and utilization of the knowledge with good professional practice. We have a growing number of Institutions producing an exponential number of Ayurveda graduates in India. Yoga and Naturopathy. Unani. Legal and Ethical Regulations Related to Education in Ayurveda System of Medicine Dr. Muralikumar President. Unani. Ministry of Ayush. Siddha and Unani Education as well as the registration of practitioners in the country within the scope of Indian Medicine Central Council Act 1970. Vanitha R. Yoga and Naturopathy. Central Council of Indian Medicine is the statutory body under Ministry of AYUSH established with the objectives to setup standards and to notify the regulations in Ayurveda. India established Ministry of AYUSH in 2014 to ensure the optimal development and propagation of AYUSH systems of health care. Earlier it was known as the Department of Indian System of Medicine and Homeopathy (ISM&H) which was created in 1995 and renamed as Department of Ayurveda. 69% inhibition higher than Standard (Ibuprofen) 47. 2hrs. Panchkatu 250 mg/Kg has 21. After one hr of carrageenininj . Panchkatu 125mg/Kg Body wt. IV. Panchkatu 250mg/Kg Body wt. 2. Paw volume of all 20 rats were measured soon after injecting carrageenan.2%). III.005) and are comparable to standard Ibuprofen.An Experimental Study Prof. Evaluation of Anti Inflammatory Activity of ‘Trikatu’ (Ayurvedic Formulation) . Ibuprofen. an Ayurvedic formulation. Carrageenin 137 . After three hrs of Carrageenininj there is increase in Percent inhibition of oedema with increase in dosage of trial drug that is Panchkatu 125 mg/Kg has 20% .28%. 4hrs and 24hrs after carrageenan injection. Panchkatu 250 mg/Kg has 31. The rats of treated Group II. 20 healthy albino rats were selected randomly and divided into five groups .42% and Panchkatu 500mg/kg has 41. III. each group containing four rats were administered orally at different dosage levels.2% while last group (Panchkatu 500mg/kg) has higher percent inhibition of oedema(50 %) than standard (Ibuprofen) (42. New Delhi The Anti inflammatory activity of Panchkatu. The change in paw volume of Group I was compared with Group II. IV & V Group. Ministry of AYUSH. III. IV and V were administered orally with Ibuprofen soln. Panchkatu 500mg/Kg Body wt. Government of India. V were also compared in between and expressed as percentage oedema inhibition by the drug. Summarizing the above it is concluded that Panchkatu has showed its extreme utility or significance on the inflammation probably because of its excellent activity of inhibiting the both early released and late released mediators which is rarely seen in any anti inflammatory formulation Keywords: Anti inflammatory. 3. 3hrs.Both Trial group Panchkatu 125mg/Kg and Panchkatu 250mg/Kg have equal percent inhibition of oedema as with Standard group (Ibuprofen) that is 42.24%. respectively one hour before injecting 1% w/v suspension of carrageenan (0. Amnish Verma Vaidya-Scientist Fellow. Carrageenan induced paw oedema is the most commonly used method in experimental pharmacology. The volume was again measured after 1. After two Hrs of carrageenaninjection there is an increase in Percent inhibition of oedema with increase in dosage of trial drug that is Panchkatu 125 mg/Kg has 27. The inflammatory reaction is readily produced in rats in the form of paw oedema with the help of irritants. The maximum activity of all trial Groups were observed during first and second hour and the results are significant ( P<0. Panchkatu. was performed on albino rats of Carrageenan induced model.49% and Panchkatu 500mg/kg has 56.19% . Inhibition was seen after 1 & 2 Hrs. Also the treated Group II. Former Technical Consultant (WHO).1 ml) into the sub plantar region of left hind paw of all the five groups.42% inhibition higher than Standard (Ibuprofen) 39. 4 & 24 hrs in all the five groups of rats. Results of the present study are based on the oedema of hind paw of rats of all five groups measured after 1hr. 138 . Track 12 Use of modern science and technology in traditional medicine: product development. diagnosis and treatment Abstracts of Keynote Speakers and Oral Presenters 139 . the exact causes of migraine are unknown and there are no recognisable. the number of identified genetic risk loci associated with common migraine has expanded considerably. Institute of Health and Biomedical Innovation. and 1st among neurological disorders). migraine is one of the most prevalent (14%). Prof. migraine attacks rarely occur spontaneously in the absence of any possible environmental trigger factors―a recent study found 95% of migraineurs associate trigger factors with their migraine attacks. Queensland University of Technology. Australia Throughout the world. 140 . Yet. Dale R Nyholt Associate Professor Statistical and Genomic Epidemiology Laboratory. In the past few years. disabling (7th leading cause of years of life lost to disability. has shown that common migraine is associated with markers related to dysfunction of various pathways. pathological changes. A number of genes involved in rare and severe sub-types of migraine have been identified. misdiagnosed. This presentation will focus on how such large molecular geneticstudies of migraine can provide new insight into mechanisms that underlie migraine symptoms and action of traditional medicines. diagnostic. metal ion homeostasis and neuronal migration. and undertreated disorders. 3rd cause in under 50s.674 migraine cases and 316.Molecular Genetics of Migraine: New Insight into Mechanisms that Underlie Migraine Symptoms and Action of Traditional Medicines Assoc. The heritability for migraine is estimated at approximately50%. indicating both genetic and environmental factors play an important role in its development. and has an enormous socio- economic burden (highest of all neurological conditions). Queensland. including vascular- related pathways. In fact. which analysed 59. the largest ever migraine genetics study.078 controls and detected 44 (34 new) independent genetic risk variants for migraine. Recently. Epigenetic changes and Brainstem Dysfunction in Neuropsychiatric Disorders – AD/PD/Anx Prof. of brainstem involvement in neurodegenerative disorders. Steinbusch Dept.M. The Netherlands Alzheimer’s disease (AD) and Parkinson Disease (PD) are severe neurodegenerative disorders resulting in progressive cognitive and motor impairments. the role of the brainstem has received relatively little attention in most neuropsychiatric disorders.Neuroscience. Parkinson Disease. in neurodegenerative diseases. attenuate or possibly reverse the pathophysiology of these disordersThe current presentation aims to review the functional neuroanatomy of the brainstem as well as the current status on findings. Maastricht University Medical Centr. cellular and imaging technologies. autonomic nervous system activity as well as regulating all higher cerebral functions via neurotransmitter projections systems originating in the brainstem. Improved insight in the cellular and molecular characteristics of brainstem function as well as the link between stress. Despite the fundamental role of the brainstem in regulating vital functional abilities such as arousal. prevent. epigenetic dysregulation and the progression of AD and PD. Medicine and Life Sciences.e. Progressive Cognitive Impairments 141 . Previous work indicates that so-called epigenetic mechanisms (i. reversible changes to the DNA induced by the environment) represent critical factors in the development and course of AD. Faculty of Health. Neurodegenerative disorders. breathing. may lead to novel targets to better diagnose. a brain region known to be critically important for the regulation of the stress response. the early occurrence of various neuropsychological symptoms points towards a key role for the brainstem. Maastricht. Keywords: Alzheimer’s disease. derived from a wide range of studies using molecular. Harry W. Moreover. The efficacy of IF to protect the brain against neurodegenerative diseases and ischemic injury involved the coordinate upregulation of multiple neuroprotective proteins including neurotrophic factors. IL-1 and IL-6) and suppression of inflammasome activation at the site of injury. Thiruma V. In conclusion. Keywords: Intermittent fasting.Intermittent fasting has been proven to extend lifespan and decrease the development and severity of age-related diseases such as cardiovascular (e. Experimental Stroke and Inflammation Research. such as SOD and HO-1. Department of Physiology. Type 2 diabetes mellitus and myocardial infarction) and neurodegenerative diseases (e. 3h. Cohorts of mice in each diet group were then subjected to sham surgery (no ischemia) or 1 hour of middle cerebral artery occlusion and reperfusion. 24h and 72h of reperfusion and genome-wide mRNA expression was quantified by RNA sequencing. intermittent fasting preconditions the brain by inducing neuroprotective pathways and concurrently preventing neurotoxic pathways. Mice were randomly assigned to ad libitum feeding and 16 hours/day fasting groups. such as BDNF and bFGF. upregulation. 12h. neuroprotective proteins. Arumugam Associate Professor. Intermittent fasting for 16h/day significantly induced pathways involved in neuroplasticity and prevented the post-stroke induction of pathways involved in inflammation and cell death compared to ad libitum group. Intermittent Fasting and Ischemic Stroke Prof. down regulation of pro-inflammatory cytokines (TNF. We have recently performed RNA-Sequencing in order to understand global mechanisms of protection by IF following stoke. Yong Loo Lin School of Medicine. neurotrophic factors. National University of Singapore Intermittent fasting (IF) is a dietary protocol where energy restriction is induced by alternate periods of ad libitum feeding and fasting. stroke. antioxidant enzymes. Alzheimer’s disease and Parkinson’s disease). protein chaperones. Mid-section of cerebrum tissue in the ipsilateral side of the brain was harvested at 0h. including Hsp70 and GRP78.g.g. neurotoxic pathways 142 . In light of these data and to promote the use of these herbs for health benefits. Japan Mortalin/mthsp70 is a stress chaperone. Intracellular trafficking of proteins. cancer aggressiveness and metastasis. Furthermore. a popular sweet viscous substance produced by honey bees from the tree barks they love onharbor anti-mortalin bioactives. we have generated Active Ingredient Enriched Ashwagandha extracts and PP- Propolis (Pleasant and Premium Propolis) that possess high stability and lack repulsive odor. Human Carcinogenesis Involves Mortalin Up-regulation: Its Management by Herbs Dr. Sunil Kaul Chief Senior Research Scientist. and factors involved in epithelial-mesenchymal transition (EMT) have been linked to cancer cell stemness suggesting that it is a potential target for stress and cancer diagnosis and therapy. Helicteres angustifolia. All of these are tightly related to carcinogenesis. activation of telomerase. It interacts with tumor suppressor protein. ionizing radiation and other hyper-pigmentation stresses. Enriched in all cancer cells and tissues. These include Withanolides (Withaferin-A and Withanone). first discovered in our laboratory while screening for proteins associated with cellular mortal or immortal phenotype. mitochondrial import. We found that these are capable of disrupting mortalin-p53 complexes resulting in reactivation of tumor suppressor activities of p53. p53 in cancer cells and causes inactivation of its transcriptional activation. and is involved in control of cell proliferation. Indeed. it is upregulated in response to glucose deprivation. National Institute of Advanced Industrial Science & Technology (AIST). mortalin siRNA was shown to possess anti-cancer potential imploring the interest in NEW (Natural Efficient and Welfare) anti-mortalin compounds. control of centrosome duplication and apoptosis activities. Cucurbitacin B and Caffeic Acid Phenethyl Ester (CAPE). ROS production. Apoptosis 143 . Although not responsive to temperature changes. Biomedical Research Institute. downregulation of mortalin and several other key regulators of cell migration accountable for its anti-metastasis activity were detected. ROS production and apoptosis. We present evidence thatherbs including Withania sominifera. and were supported by in vivo tumor suppressor assays. Other activities of mortalin such as. hnRNP-K. intracellular trafficking of proteins. it has been shown to play life-essential functions including chaperoning. Keywords: Up regulation. and propolis. DN Karunaratne2 1 Department of Natural Products Chemistry. Syzygiumaromaticum. HS Williams4 1 Department of Basic Sciences. with the nanoparticles. University of Peradeniya.OP 12-01: Linamarin encapsulated Chitosan nanoparticles: Preparation. it was delivered and internalized more effectively than free linamarin. The cytotoxicity of free linamarin and linamarin loaded chitosan nanoparticles were assessed by SRB assay. periodontitis and oral cancer. BP Galhena3. linamarin. antibacterial and genotoxic effects. Faculty of Dental Sciences. Sri Lanka 3 Department of Biochemistry and Clinical Chemistry. Cells were exposed to different concentrations of linamarin loaded into chitosan nanoparticles and free linamarin for 24. Sri Lanka 3 Sri Lanka Institute of Nanotechnology. characterization and in vitro release studies IHV Nicholas1. V Karunaratne2. MP Paranagama1. Sri Lanka 2 Department of Chemistry. Ethyl acetate extract of this mouth freshener was analyzed for antioxidant effects using ferric reducing antioxidant power (FRAP) and DPPH and ABTS radical scavenging activities. TEM and SEM techniques.48 μg/mL while it was 373. Kaempferia galangal and Coriandrumsativum is claimed to prevent bacterial and/or oxidative stress associated oral diseases such as dental caries. In vitro cytotoxicity results revealed that when linamarin was loaded into nanoparticles. Elettariacardamomum. The IC50 value after 72 h for linamarin loaded chitosan nanoparticles was 26. 48 and 72 hours. University of Peradeniya. MRDM Senanayake2. Areca catechu. The objective of this study was to analyze this mouth freshener for its antioxidant. Faculty of Science. nutraceuticals. Sri Lanka In Sri Lankan folklore. In this study the in vitro cytotoxicity of linamarin loaded chitosan nanoparticles were evaluated using MCF-7 cells. thermo gravimetric analysis (TGA). The successful loading was confirmed by FT-IR and TGA results. Sri Lanka 2 Department of Oral Medicine and Periodontology. nanoparticles. FT-IR. Cell viability was decreased to about 40-50% after 48 h and to about 24-38% after 72 h. Sri Lanka Nanoparticulate delivery of nutraceuticals provides a new insight in prevention and therapy for cancer. University of Kelaniya. GAJ Amaratunga3. JAMS Jayatilake2. Antibacterial effect was analyzed 144 . Keywords: chitosan. Medical Research Institute. Synthesized nanoparticles were around 50 nm with higher positive zeta potential and higher encapsulation efficiencies (85%). Linamarin. Faculty of Medicine. University of Kelaniya. Linamarin loaded chitosan nanoparticles were obtained by ionic gelation between chitosan and sodium tripolyphosphste and characterized using dynamic light scattering. Faculty of Dental Sciences.50 μg/mL for free linamarin. University of Peradeniya. Myristicafragrans. found in cassava was proven as a drug candidate for cancer treatment due to its cyanogenic property. In free linamarin. Sri Lanka 4 Department of Pathology. Faculty of Medicine. The morphological observations obtained by inverted microscopy were in good agreement with those results. Time dependent cytotoxicity was highlighted more than dose dependent cytotoxicity in linamarin loaded chitosan nanoparticles. a poly-herbal mouth freshener which consists of Piper betle. 50% inhibition of cells was not achieved even after 72 h. MCF-7 cells OP 12-02: A non-genotoxic traditional polyherbal mouth freshener rich in natural antioxidants and antimicrobials NS Piyarathne1. 0±0mm) respectively for Streptococcus mutans and (18mm±1. antioxidants. Lower IC50 value indicates higher antioxidant activity. Faculty of Medical Sciences.2mm). mutans. The quality of the essential oil was compared with a commercial sample using Gas chromatography.5 mg/ml of the same extract showed zones of inhibition of (14. University of Sri Jayewardenepura. Genotoxicity was analyzed using cytokinesis block micronuclei (CBMN) assay on isolated human lymphocytes. RD Gunaratne2.2-diphenyl-1-picryl- hydrazyl-hydrate (DPPH) assay and BHT was used as the positive control.5±0. Ethyl acetate extract of this mouth freshener was found to be a rich source of natural antioxidants ((FRAP: 4. Contact allergy to surgical gloves is also caused by antioxidants that are used to prevent rubber degradation. Faculty of Science. Future studies will be concentrated to investigate a suitable percentage 145 .7mm) respectively for Porphyromonasgingivalis.23±0. (12.0±0mm). In the present study cinnamon leaf oils were extracted using Clevenger apparatus and the percentage yield of the essential oil was 3. Sri Lanka The number of surgical/examination gloves used globally in health care has increased dramatically and allergic contact dermatitis develops on the hands after exposure to latex examination gloves. gingivalis. A number of plant secondary metabolites act as scavengers of free radical species and so have been classified as antioxidants. University of Kelaniya. DPPH assay as IC50:25. we conclude that the ethyl acetate extract of this traditional polyherbal mouth freshener is a non genotoxic source of natural antioxidants and antimicrobials and propose it as a potential candidate to be used in preventive oral health care after clinical trials. Agar well diffusion assay with 30. Cinnamon zeylanicum is an endemic plant in Sri Lanka and the essential oils from bark and leaf are heavily used in perfume and food industries. Sri Lanka 3 Department of Chemistry. ABTS assay: 2. The present study aims to describe the potential of cinnamon leaf oil as an alternative natural antioxidant in the glove industry. P. 15 and 7.7±1.33 μg/mL) which is three times less than the synthetic antioxidant BHT (24. (15. Faculty of Technology. The cinnamon leaf oil has the highest antioxidant activity (IC50 = 8. Keywords: traditional polyherbal mouth freshener.2 %. It was confirmed that both samples contained 78 -80 % eugenol.3±1.50±0.5 mg/ml.03 mmol of Trolox equivalents /mg of DW). Thus. The radical scavenging activity of cinnamon leaf oil has been evaluated using 2. Surgical glove chemicals are the most frequent causes of occupational allergic contact dermatitis in health care workers as a result of frequent glove use. S. Sri Lanka 2 Department of Biosystems Technology.27 µg/ml. CBMN assay OP 12-03: Method of incorporation of natural antioxidant from cinnamon leaf oil as an antioxidant into surgical and examination gloves S Abira1.7mm) (13. The results revealed that 100 % radical scavenging activity was shown at 10 µg/mL concentration of the essential oil. University of Sri Jayewardenepura.using agar well diffusion assay.13 mmol Fe+2 equivalents /mg of DW.0±1. KRD de Silva1 1 Interdisciplinary Center for Innovations in Biotechnology & Neurosciences.3mm) and (12. Therefore it is confirmed that cinnamon leaf oil can be used as the antioxidant to develop surgical/examination gloves.09 μg/mL). PA Paranagama3. CBMN assay revealed that this extract is not genotoxic even at a very high concentration of 2. University of Ruhuna.002) tests. Faculty of Medicine.of the stabilizer and the emulsifier in order to prepare a homogeneous solution of the essential oil to be used in the surgical/examination glove manufacturing process. Curcuma longa. Petroleum ether 4mg/ml extract in PT test (p=0. nervosa in both PT (p=0. This study demonstrates that Argyreia Nervosa can be considered to develop as an anticoagulant drug. which is used in the traditional medicine system of Sri Lanka for its anticoagulant activity. elephant creeper in English. University of Ruhuna. The whole plant of A. Faculty of Medicine. activated partial thromboplastin time test. surgical/examination glove. Plant extracts obtained from Citrus aurantifolia. Keywords: Argyreia nervosa. University of Peradeniya. F3. Keywords: antioxidant.show statistically significant prolongations when compared to controls according to one way ANOVA model. nervosa possesses a statistically significant anticoagulant activity compared to control. All the prepared extracts were screened for anticoagulant activity in Prothrombin time (PT) test and Activated Partial Thromboplastin Time (APTT) test using plasma samples from 10 healthy volunteers for each concentration. Nervosa was extracted in methanol. as a preliminary study for developing an anticoagulant drug. nervosa produce anticoagulant activity or it possesses one compound which can inhibit the coagulation cascade at more than one site. and ethyl acetate. F1. KRS Neelanayani2. WMDGB Wijayaratne3 1 Pharmacy programme. Aloe vera. F2. Sri Lanka 2 Pharmacy programme. DBM Wickramarathne Faculty of Allied Health Sciences. In vivo 146 .005) and APTT (p =0. Faculty of Medicine. Azadirachta indica. Sri Lanka The present study was carried out to formulate five different polyherbal handwashes followed by evaluation of in vitro antibacterial efficacy and safety. Warfarin and heparin were used as positive controls for the tests. The stability of the five different formulations was studied by evaluating the pH and physical appearance for one month. Escherichia coli) bacteria by using a well diffusion method. anticoagulant OP 12-05: Formulation of a poly-herbal hand-wash with potential antibacterial activity SK Hettihewa1. Cymbopogon citrates and Sapindus trifoliatus in different ratios were incorporated into the hand-wash base to prepare five different formulations. petroleum ether. prothrombin time test. Sri Lanka The aim of this study was to screen Argyreia nervosa (Maha-Dumudu in Sinhala. Sri Lanka 3 Department of Microbiology. Crude 4mg/ml extract in APTT test (p=0. F4 and F5. University of Ruhuna.039) and ethyl acetate 4mg/ml extract of A. Efficacy of antibacterial activity of all five formulations were evaluated against Gram positive (Staphylococcus aureus) and Gram negative (Pseudomonas aeruginosa. It is concluded that A. This study suggests that the multiple active constituents in A. The crude extract was partitioned with solvents. Ghav-patta in Hindi).037). essential oil OP 12-04: Preliminary study for developing an anticoagulant drug using herb Argyreia Nervosa MHAT Madhushani. A concentration series was prepared by dissolving each extract in 1% Phosphate Buffer Saline (PBS). water. 6. Keywords: anti-cancer drugs. The calculated BDE of LinD-1 is 53. aureus. in-vitro.3±0.6) and F3 (S. Escherichia coli. Polyherbal handwash OP 12-06: In silico design of Linamarin based anti-cancer drugs A Dayananda.3±0. 12.02 kcal mol-1.5. University of Sri Jayewardenepura. aureus. Therefore. Rational changes were then made to the linamarin structure to reduce the bond dissociation energy (BDE) of the glycosidic bond without compromising the binding affinity. aeruginosa. in-vivo. This study was focused on rationally modifying linamarin to a more potent anti-cancer drug by enhancing the rate of liberation of HCN.5±0. 23.00 kcal mol-1 where as that of linamarin is 56. Linamarin was docked to linamarase that was previously equilibrated with 100ns long Molecular Dynamics simulations (MD).6±1. The results obtained from the stability test showed that F1.0±0. It is concluded that formulation F3 is a polyherbal hand-wash with potential antibacterial activity that should be marketed as a healthcare product in Sri Lanka. Sri Lanka Cassava has been used in ancient traditional medicine to cure various diseases. medicinal plants. The in vivo efficacy test revealed that F3 was the most active (92%) on colony reduction percentage compared to that of the commercial product tested (48%). Chemical breakdown of linamarin linamarase and other related enzymes liberate toxic HCN which cause cell apoptosis of cancer cells but not of healthy cells. this study proposes that LinD-1 is a better anti-cancer drug than its natural scaffold linamarin. the stability of the complex and the important enzymatic contacts were identified by analyzing the MD trajectories. 10. P. F3 and F5 are the most stable formulations. The newly designed linamarin derivative (LinD-1) has a compatible binding free energy with linamarin towards linamarase. 8. P. RS Jayakody Department of Chemistry. a cyanogenic glycoside and it is a confirmed anti-cancer agent. Keywords: antibacterial efficacy. 15 and 30 days) to test the efficacy of the polyherbal hand-wash (F2 and F3). 11.8±0. color retaining property and antibacterial activity.6. The active compound in cassava is linamarin. 15. 23. F2.4.6) compared to the commercial product (S. F2 and F3 were selected for further studies.antibacterial efficacy test was performed on healthy volunteers (n=40) for one month (1.6±0. aeruginosa. By considering stability. aureus. the hypersensitivity/ allergic reactions did not appear on the volunteers for one hour. 16. linamarin 147 .2. 7. aeruginosa.1±0. The mode of binding of linamarin to linamarase. Escherichia coli.6. The greatest inhibition was in the formulations of F2 (S. P. Furthermore. cassava.3±0. Escherichia coli.2) tested. This reduction in BDE allows LinD-1 to produce a hundred times more HCN per given period of time. The volunteers were further observed for one hour for the appearance of any kind of hypersensitivity/allergic reaction under the supervision of a registered medical practitioner. 00 kcal mol-1. Further. Further. Hence.based cancer analgesics S Fernando.76% reduction in total binding energy compared to TTX. TTX-D1 shows 86% reduction in electrostatics and 34. A homology modeled VGSC was relaxed with a 100 ns Molecular Dynamic (MD) simulation prior to docking of TTX. In contrast.OP 12-07: In silico designing of neurotoxin.52 kcal mol-1respectively. University of Sri Jayewardenepura. Sri Lanka Tetrodotoxin (TTX) is a lethal neurotoxin found in some marine creatures that blocks the trans- membrane voltage gated sodium channels (VGSC) and ceases nerve impulse transmission leading the victim to death. R Jayakody Department of Chemistry. This study was focused to designing aTTX-derivative which can be used as an effective painkiller. neurotoxin-based drugs. these numbers for the novel TTX derivative (TTX-D1) are -32. The estimated binding free energy of TTX to VGSC is – 49. with an electrostatic contribution of -19. Keywords: TTX . structure manipulations of TTX were also found with the same objective. no TTX-based analgesics are available yet. The TTX molecule was modified to a painkiller by systematically disabling its electrostatic ‘hot spots’. cancer pain .22 kcal mol-1and -2. further developments in TTX-D1 as a powerful analgesic for cancer pain is proposed.39 kcal mol-1. Knowledge of the binding modes in the VGSC-TTX complex were used to rationally modify the guanidium moiety of TTX to reduce its negatively charged regions. asit hinders the propagation of pain signals with much less toxicity than TTX. There are many records in the literature where dose optimization of TTX was attempted for its medical usage in humans. cancer pain killers 148 . Nonetheless. Abstracts of Poster Presentations 149 . Triphalā and Mimusops elengi (Mūnamal) aqueous extracts against Candida and selected bacterial species were determined by agar well diffusion method. Sri Lanka is proud for its rich indigenous medical knowledge for the management and prevention of cancers. apoptotic activity.2% Chlorhexidine Gluconate. flavonoids. pilika. Herbs have a vital role in the prevention and treatment of cancers. Staphylococcus epidermidis and Methicillin-Resistant Staphylococcus aureus (MRSA). Kuwait This study was aimed to determine the effect of 0. Published three book series of them was searched for remedies used for gedi. Embellica officinalis. Kuwait University. University of Sri Jayewardenepura. LP Samaranayaka4 1 Department of Microbiology. Sri Lanka and Department of Medical Laboratory Sciences. University of Kelaniya. MM Weerasekera1. Piper longum is the most commonly used for pilika. Anti-oxidant activity. These medicines are noted to have the potential to suppress neoplasm growth. According to selected books. aggresive function and enhance immune functions. Sri Lanka and Sri Lanka Institute of Nanotechnology 4 Department of Bioclinical Sciences. three gram positive cocci. This research was to evaluate the potential natural sources having anti-cancer activity. In recent years because of the fear of side effects. anti-tumor activity and anti-inflamatory activity are common therapeutic actions of these anti-cancer herbs. gram negative bacilli Escherichia coli and Pseudomonas aeruginosa. A total of 33 popular and plentiful herbs for cancers were selected and was analyzed for bio-active chemical compounds and therapeutic actions of them. people all over the world show preference to use natural products for cancer treatment. N Kottegoda3. University of Sri Jayewardenepura. Zingiber officinale. In indigenous ola leaf manuscripts. Sri Lanka The plant sources of Sri Lanka are likely to be effective anti-cancer agents. University of Sri Jayewardenepura. Sri Lanka 3 Department of Applied Sciences. Piper longum. Piper nigrum. Sri Lanka 2 Department of Microbiology. Minimum inhibitory concentration (MIC) of Candida glabrat and Candida dubliniensis was 150 . bio-active compounds. vana. Staphylococcus aureus. Cuminum cyminum. pilika conditions. vana. there are remedies for various gedi. SSN Fernando1. The effect of 0. therapeutic actions PP 002: Tripalā and Mūnamal – useful antimicrobial agents against selected bacterial and fungal microorganisms PDTA Jayarathna1.2% Chlorhexidine Gluconate. anti-neoplastic activity. GK Wijesinghe2.PP 001: Survey study on Pilika nashaka (anti-cancer) drugs in Sri Lankan indigenous medicine UPT Uluwaduge Department of Cikitsa. traditional use. General Sir John Kotelawala Defense University. Phenolic compounds. TDCP Gunasekara1. This was followed by collection of popular herbs and minerals from 10 Sri Lankan Traditional Medical practitioners. Ferula foetida. Cinnamomum zeylanicum are the other commonly used plants. Faculty of Allied Health Sciences. Faculty of Dentistry. Faculty of Medical Sciences. According to practical usage of selected doctors. Triphalā and Mimusops elengi (bark of Mūnamal) aqueous extracts on five Candida type strains. volatile oil and alkaloids are common bio-active chemicals in these anti-cancer herbs. Keywords: anti-cancer. essential oil. cytotoxic activity. Gampaha Wickramarachchi Ayurveda Institute. anti-microbial activity. analgesic activity. Faculty of Medical Sciences. Nigella sativa. Highest Tripalā concentration of the dilution series used (65 mg/ml) was the MIC for both organisms. given by Hippocrates. Keywords: Adun. DKK Weerasinghe. albicans and C. Keywords: bacteria.19th in Uttara tantra.A literary review on an Ola leaf manuscript HKR Weerakoon. Ola leaf manuscript. external eye applications including fifty-nine paste termed as Puravalliyam. Puravalliyam.A holistic approach of treatment in Unani (Greeko-Arab) medicine RF Rismiya. tropicalis) at 1/512 dilution (MIC = 0.004 mg/ml for both organisms). ten poly-herbal eye drops. 0. thirteen ointments known as Adun. chlorhexidine. W Weerapana Bandaranaike Memorial Ayurvedic Research Institute. where 120 were in the sclera. Safra (bile) and 151 .2% Chlorhexidine gluconate had the maximum effect against Candida and tested bacterial species followed by Triphalā and Mūnamal (Mimusops elengi) extract. also known as Greeko-Arab medicine. which supposes the presence of four body fluids. It was revealed that there are altogether 320 eye diseases described. four oils. 99 in the cornea and 101 in the external eye. One first aid treatment. Balgham (phlegm). also known as Hela Vedakama. has been practiced for many centuries. published in 1953 and the Suśruta saṁhitā chapters 7th .2% Chlorhexidine gluconate had the maximum zone of inhibition for all test strains whereas Triphalā exhibited a moderate effect on test strains except Candida glabrata. Sri Lanka The Unani System of Medicine.Dam (blood). Anti-Candidial activity of chlorhexidine gluconate was achieved at very low concentrations. one powder and three semi solid preparations known as Ghṛta. Chlorhexidine gluconate inhibited the growth of Candida (C. traditional medical knowledge PP 004: Musaffiyath (blood purification). were mentioned in the OLM. nine pills and internal medicine including nine decoctions. University of Colombo. Only Staphylococcus aureus and MRSA were susceptible to Mūnamal pothu. The purpose of the present study was to identify the diseases according to Ess Vedakama in the Indigenous medical system. C Welivitegoda. Candida dubliniensis and Escherichia coli. Firstly. NKPSS Fernando. founded by Hippocrates is based on the concept of Nazriya Akhlaat (Theory of Humours). This traditional medical knowledge is transferred continuously through generations either verbally or written in Ola leaf manuscripts (OLM). Clinical studies are recommended to strengthen the clinical use of these medications. In conclusion 0. specific for eye diseases. Mimusops elengi (Mūnamal) PP 003: Deśīya Ás Vedakama . Essvedakama. Sri Lanka The traditional medical system in Sri Lanka. the manuscript was copied by hand and then critically reviewed by comparing with the already conserved OLM Sinhala Rajunge Ás Vedapotha compiled in Ceylon National Museum’s Manuscript Series Vol: III. This is very similar to Sinhala Rajunge Ás Vedapotha but different from the Suśruta saṁhitā. The sample OLM numbered as 369 was selected from the conservation unit of the OLM in the literary research section in the BMARI.determined using broth microdilution method with alamar blue modification. Triphalā. candida. MLU Salma Institute of Indigenous Medicine. insomnia or incomplete sleep. infectious diseases. Humours. The aim of this study is to find the pharmaceutical utilities and pharmacological attributes of two different formulas of Navaratna Guliya according to traditional medical lore. diabetes. Ingredients. Keywords: Navaratna guliya. First formula of Navaratna pill is in verse form and the other one is in pose form written in Sinhala. Kaifiyat PP 005: Pharmaceutical utilities and pharmacological attributes of two different formulae of Navaratna Guliya according to Traditional Medical Lore WGNS Samanweera. hypertension and other chronic disorders. Navaratna Guliya is also a medicinal pill prescribed for acute disorders but not similar with the formula of Navaratna Kalkaya. The second formula includes 26 ingredients with 25% of Croton tiglium and 50% of castor seeds which are ground with consecutively water and lime juice. ingredients 152 . allergies. therapeutic applications and media of administration of both are different but first formula shares some common indications with Navaratna Kalka too. The imbalance in the quality (Kaifiyath) and quantity (Kammiyath) of these humours leads to diseases whereas restoration of this balance maintains health of a person. There are two different formulae found in a palm-leaf manuscript that has not been published through print media. hormonal disorders. Data for this literary work was collected from Unani textbooks and electronic Media. purgation (Mushilath) and neutralization (Muaddilath). The first one has 17 ingredients which comprise 50% of Cannabis sativa and Caesalpinia bonducin equal parts. Gampaha Wickramarachchi Ayurveda Institute. According to unani medical system Musaffiyath e khoon is one of the procedures of evacuating morbid Madda (impurified matter) from the body. Humour Dam or blood is the large portion of humours that circulates throughout the body. JMPRK Jayasinghe Department of Dravyaguna. Musaffiyaths act through four ways to purify the blood. diabetes and infections are some causes that alter the equilibrium of Damavi humour. hormonal changes. perspiration (Muarriqath). Keywords: Musaffiyath e Khoon. They evacuate morbid matters via kidney (Mudirrath). use of junk foods. Kammiyat. Navaratna Kalkaya is one of the most famous indigenous medicinal pastes used by laymen society for conditions associated with indigestion and other stomach ailments. obesity. The ingredients are prescribed to be ground with garayusha which cannot be interpreted and cannabis and triphala decoction. mode of preparation. Since there are many formulas inherited by oral tradition and written in palm-leaf manuscript that are not published in print media. literary researches need to be carried out on those unpublished sources. University of Kelaniya. formula. Findings show that improper diet. stress problems. Musaffiyath e Khoon drugs are very effective in treating skin disorders. therapeutic applications. Damavi.Sauda(black bile) known as Akhlaat (Humours). The purpose of this study was to present a holistic approach of Musaffiyaths in treating diseases. It is thus suggested that Musaffiyath can be used effectively for the management of various disorders. hypercholesterolemia. Sri Lanka Vatikaprakaranaya has compiled more than 350 medicinal pills and pastes belonged to Sri Lankan indigenous medical tradition prevailed though out the past history. Imbalances in quality or quantity of the blood cause many health hazards. drinking less water. Karuva and Karuvapatta used in Kannada and Tamil languages would have been borrowed from Sinhala word Kurundu which is transliterated into 153 . 6 (40%) of Kalka. 692mg and min. preparation method and dosage. Sri Lanka Ayurveda Pharmacopoeia of Sri Lanka (APSL) is the legally recognized and officially published authentic source of the Ayurvedic pharmaceutical preparations in Sri Lanka. There are 76 formulae (15%) containing cinnamon recorded out of 500 internally prescribed preparations in APSL. 20mg). (max. University of Kelaniya. ancient literary sources and scholarly publications on the history of cinnamon. University of Kelaniya. 8 (88%) of Pindī (max. Sri Lanka and Department of Medicine. Gampaha Wickramarachchi Ayurveda Institute. internal preparations. The percentage of cinnamon in each formula was calculated and the highest (9. 20mg). The methodology was based on lexicons. 625mg and min. 26mg) formulae contain cinnamon as an ingredient. University of Kelaniya. Sri Lanka 2 Gampaha Wickramarachchi Ayurveda Institute. The aim of this study was to analyze the diversity of proportionate distributions of cinnamon and percentages in each formula prescribed for internal use as mentioned in APSL. The average (68.98%) in was found in Vṛhat YogarājaGuggulu. University of Kelaniya.PP 006: An analysis on cinnamon containing formulae in Ayurveda pharmacopeia of Sri Lanka WGNS Samanweera1. Ayurveda pharmacopeia PP 007: Philological evidence for Sri Lanka as the origin of true cinnamon WGNS Samanweera1. It is suggested that further analysis be conducted on the pharmaceutical utilities and application diversity of the studied sample of 76 cinnamon containing preparations in APSL.09%) was found in Haridrā Khaṇḍa which is used for allergic conditions. In APSL more than 500 internal preparations are mentioned with their formula. Keywords: cinnamon. S Pilapitiya2. Sri Lanka 2 Post Graduate Institute of Archeological Research. This study aimed to trace the philological evidences to prove Sri Lankan territory as the country of origin of true cinnamon. Faculty of Medicine and Allied Sciences. University of Kelaniya.300mg as recorded in 52 formulas. Global attention on cinnamon is significantly increasing since it has been used in traditional medicine for the last millennia and scientifically proven for its efficacy on various ailments. dosage forms. R de Silva4 1 Department of Dravyaguna. DL Perera2 1 Department of Dravyaguna. 10mg). P Paranagama3. Primary data were collected for APSL and scholarly opinions were consulted for analytical study. Gampaha Wickramarachchi Ayurveda Institute. Statistical analysis shows that 15 (24%) of Cūrna (max. Sri Lanka 4 Interdisciplinary Centre for Innovation in Biotechnology & Neuroscience. while the lowest (0. 390mg and min. 11(68%) of Modaka (max.42%) weight range of cinnamon in dosage forms is between 100mg . Sri Lanka Cinnamon or Kurundu tree is reported to have originated in the central hills of Sri Lanka and Ceylon cinnamon is one of the earliest spices known to mankind and is traded around the world. 160mg and min. Rajarata University of Sri Lanka 3 Department of Chemistry. The botanical name Cinnamonum zeylanicum Blume refers to the cultivated specific species of cinnamon indigenous to Sri Lanka and that is also called “true cinnamon” of commerce. Most of these preparations are prescribed to be taken twice a day and maximum cinnamon content was recorded as 692 mg in single dose of Abhayādīmodaka. RWKH Rajasekara. The present study findings refer to Sarvanga Vedakama mentioned in the OLM 363 and 564 including different recipes for different ailments. Korean. Finnish. Powder. Basna. Malayalam. Chinese. transferred either verbally or written in Ola Leaf Manuscripts (OLM). The purpose of this study was to conserve and popularize the Traditional medical knowledge through documentation and review. which means ‘belonged to the country of Sinhala’. Ess and Vidum Pliissum Vedakama. In ancient times the island was named in chronicles as Sihala-dīpa which had been transliterated into Arab as Serendib. YNDS Rupasingha. Singaldip and Sheelan. In Sarasvati Nighandu. Qurfa used for cinnamon might have etymological connection with the early form of Sinhala word Kurundu. The Arabic words Qarfah. French. PKIC Paduwawala. Sri Lanka Traditional medical knowledge in Sri Lanka refers to the knowledge with respect to medicine. three types of Kalka. Kalka. specific to OLM 564. Polish. The solid. Ola leaf manuscripts. Ghṛta. Slovak. and Vietnamese also use words connected with Ceylon. a medical lexicon cinnamon is referred to as Saimhala. Kayam and Oil were traced in the manuscripts whereas most of the recipes in OLM 363 were very similar to Vatika Prakaranaya. five types of Cūrna and two types of oil preparations were not found in Vatikaprakaranaya. Bandaranaike Memorial Ayurveda Research Institute. It was revealed that the OLM no 363 is a compilation of three aspects in indigenous medicine known as Sarvāṅga. German. LM Yaseer Bandaranaike Memorial Ayurveda Research Institute. However the four types of Pills.English as Coorundo in 17th century records. IN Hewapathirana. thereafter critically reviewed comparing with the already written old text Vatikaprakaranaya by Ileperuma in 1933. DVSPM Vidyathilaka. The sample of two OLM numbered 363 and 564 referring to general medicine (Sarvāṅga Vedakama) were selected from the conservation unit of the OLM in the literary research section. S G Kisholorjan. W Weerapana. semi solid and liquid preparations termed as Pills. LHIP Chandrawansha. Qirfah. PHV Jayasinha. Thai. The names Illavangam and Lavanga Patta used for cinnamon in Kannada. Tamil and Telingu derived from the term Lavanga which had been used as a synonym in native form “Lamanga” for both cinnamon and clove. The effective herbal formularies could be traced by referring OLM and further research is needed to validate their clinical efficacy in the system of indigenous medicine. Lamanga. Saimhala. Russian. Keywords: kurundu. Keywords: indigenous medicine. RSH De Zoysa. Serendip. traditional medical knowledge Sarvāṅga Vedakama 154 . Hungarian. Serendiv. HMWR Bandara. They were firstly copied by hand by the two groups of intern medical officers during 2016 Dec to 2017 April. Turkish. Estonian. Other languages like Belarusian. true cinnamon PP 008: Documentation and review of traditional medical knowledge from selected Ola leaf manuscripts C Welivitegoda. Sri Lanka 2 Department of Oral Medicine and Periodontology. The objective of present study is to determine different bioactivities including DPPH radical scavenging assay. N Alles1 1 Department of Biochemistry. anti-inflammatory PP 010: Aqueous extract of Salacia reticulata leaves can increase bone formation in-vitro K Godakumara1. University of Kelaniya. Staining intensity was measured using ImageJ image analysis software. Antibacterial assay and Human red blood cell (HRBC) membrane stabilization assay of different parts of Piper longum namely leaf. Faculty of Medicine. University of Peradeniya. has been used as a therapeutic agent in indigenous medicine specially in Ayurveda as a remedy for inflammation. Keywords: Piper longum. matured fruit. Salacia reticulata aqueous leaf extracts presented a significant (p<0. PA Paranagama2 1 College of Chemical Sciences. von Kossa staining and quantitative PCR analysis on specific mRNA expressions such as ALP. Sri Lanka 2 Department of Chemistry. The extraction process was carried out according to cold and hot extraction methods.inflammatory activity of indigenous medicinal plant Piper longum PDTCP Peramuna1. The results obtained from Anti-inflammatory activity depicted that all plant extracts derived from cold extraction and immature fruit extract derived from hot extraction process showed high percentage inhibition activity than Aspirin. Sri Lanka Health of the bone tissue depends on bone formation caused by osteoblasts and bone resorption caused by osteoclasts. Murine calvaria derived primary authentic osteoblasts were exposed to a crude aqueous extract of Salacia reticulata leaves with untreated controls and the effect on osteoblast differentiation and function were measured using alkaline phospotase staining. The DPPH radical scavenging activity results revealed that stem extract obtained from cold extraction and leaf extract obtained from hot extraction showed high activity.bacterial activity and anti. Faculty of dental Sciences. immatured fruit. the standard drug used. Institute of Chemistry Ceylon. G Thiripuranathar2. The stem and immatured fruit extract derived from hot extraction showed inhibition against Bacillus subtilis. Sri Lanka Piper longum. The current study was conducted to investigate the feasibility of using Sri Lankan traditional medical cures as a single agent therapy against such conditions. Ethyl acetate was used in cold extraction process. anti-oxidant. C Premathilake1. Deviation of any of these processes would lead to serious conditions such as osteoporosis for which no single therapeutic agent is yet discovered. The difference was considered significant when p<0. and stem.PP 009: Determination of anti-oxidant activity. The results of antimicrobial activity revealed that leaf and stem extracts obtained from cold extraction exhibited activity against Escherchia coli and Bacillus subtilis. N Soysa2. The statistical significance of difference among groups was assessed using one-way ANOVA. osteopontin and collagen I. antibacterial. University of Peradeniya. P Fernando1. popularly known as ‘Thippili’ in Sri Lanka.05. Soxhlet apparatus was used in hot extraction and methanol was used as the solvent. anti .005) increase of osteoblast differentiation bone matrix mineralisation 155 . Asuri (surgical).0% Concentration of M3. and 12. osteoblast PP 011: A comparative antimicrobial study of Mrityunjaya Rasa MVR Wijayanthamala Gampaha Wikramarachchi Ayurveda Teaching Hospital. 10%. Variation in the results of antibacterial activity of sample M1. Manushi (Herbal) and Daivi (Metalo.mineral). It can be concluded that S. treatments have been classified under three categories.5%) of the samples M1.M2.0% concentration of M3 against Pseudomonas aeruginosa. E. After this. For this study. Formulation M2 found to be highly effective than other formulations. No sensitivity was observed at 5. DMSO PP 012: Quantification of in vitro antioxidant activity of Abelmoschus moschatus leaf extract using spectrophotometric methods AMSS Amarasiri. Streptococcus pyogenes was highly sensitive to 12.0% Concentration of M2. against Streptococcus pyogenes. the above bioactivities were not scientifically proven. Daivi Chikitsa has considered as a best treatment therapy. Faculty of Medicine. Keywords: Daivi Ckitsa.0% Concentration of M1 against Salmonella typhiandStaphylococcus aureus and only 5. moschatus are documented with nephroprotective and antioxidant activities. The leaves and seeds of A. The aqueous refluxed (4 h) leaf extract of A.5% Concentration of M3. M2 & moderately sensitive to 12. University of Ruhuna. KAPW Jayatilaka. M3 except 5. M3 were prepared in DMSO. However. coli and Staphylococcus aureus. So. Sri Lanka Abelmoschus moschatus (Kapukinissa. 156 . Mritynjaya Rasa.M3. Mrityunjaya Rasa is a drug of choice for general infective diseases along with fever. M2. M3 could be attributed due to Bhavana dravya. moschatus using three standard assays. Sri Lanka In an Ayurvedic classical text. 5. E. bone matrix mineralisation and nodule formation in vitro.coli. this study was undertaken to evaluate the total antioxidant potential of A. and Ayurveda sara sangraha – M1. to evaluate its anti-microbial activity against common pathogenic bacteria and comparative anti-microbial study to evaluate the role of levigating liquids: three samples were prepared as per reference of AFI.and nodule formation compared to untreated cells. reticulata leaf aqueous extracts significantly increase osteoblast differentiation. Pseudomonas aeruginosaand Salmonellatyphi was moderately sensitive to all concentration of M1. It can be recommended that further studies be conducted to identify the active molecules and the mode of action in these extracts. Keywords: osteoporosis. Hence. Yogaratnakara . Bhavana Dravya. In-vitro studies were undertaken to assess the antimicrobial activity. Well diffusion method was employed. M2. All sample of Mrityunjaya Rasa have antibacterial activity against common pathogens. Staphylococcus aureus. M2. Salacia reticulata. Family:Malvaceae) has been widely used for the treatment of kidney diseases in traditional Ayurvedic medicine. AP Attanayake. different concentration solutions (5%.5% concentration of M1. According to MTT assay the extract was not toxic to cells in the functional doses (50 µg/ml). eco-friendly 157 . TR Kaushalya1. Manisha Deb Mandal et al. But traditional medical practitioners (TMP) inherit prescriptions including CS as an herbal ingredient. moschatus resulted in increased antioxidant activity in vitro. The high polyphenol and flavonoid contents of the aqueous leaf extract of A.7 ± 0. rakta shodhaka. hepatoprotective. FRAP (9. As external treatments. moschatus possesses significant antioxidant activity against DPPH radical scavenging (IC50– 63. cardioprotective and immune-stimulatory properties. traditional medicine.33 µg GAEg-1dw and 2. Sri Lanka Coconut (Cocos nucifera: Aracaceae) is one of the major plants in Sri Lankan plantation industry.11 µg QE g-1dw. Therefore further studies are essential to validate the medicinal value of CS in order to potentiate the global scenario towards greener products and to enhance the sustainability and eco-friendly society.2 µg/mL). Institute of Indigenous Medicine. The antioxidant activity was determined by 2. Considering the results it can be justified that CS contains sangrahaniya. Chemical Medicinal and Cosmetic Science etc. antioxidants. The total polyphenol and total flavonoid contents of the extract were 1. respectively. 2011 has proven that coconut contains antimicrobial. there are not enough evidences regarding the medicinal utility of coconut shell (CS) in Nigantus and other Ayurvedic literature on pharmacodynamics. flavonoids PP 013: A survey on utility of coconut shell in medicinal prescriptions of traditional medical practitioners of Sri Lanka GTR Madushani1. University of Colombo. The effectiveness of CS has been discussed under numerous schemes as Industrial. 11% in nasya karma and 56% for skin disorders. PHM Lakmani1. Though every part of coconut has been used for various medicinal requirements. Consequently 33% TMP have been used CS for external treatments and 10% of them in internal treatments. MCK Fernando1. CS is used for orthopaedic conditions by 33%. University of Colombo. Sri Lanka 2 Department of Deshiya Chikitsa.moschatus was used in the study. Total polyphenol and total flavonoid contents were estimated using Folin-Ciocalteu method (reference compound: gallic acid) and aluminum chloride method (reference compound: quercetin) respectively. ferric reducing antioxidant potential (FRAP) assay and thiobarbituric acid (TBA) method spectrophotometrically (reference compound: L-Ascorbic acid). An interview based survey was done among 30 of TMP regarding the utility of CS in prescriptions. 2’-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging assay.05 ± 0. Keywords: coconut shell.12 µM) and TBA (9. medicinal value. This study was an attempt to identify the utilization of CS in traditional medicine in order to emphasize the pharmacodynamic properties under clinical surrounding and to encourage the society to improve the sustainability through eco-friendly utility of CS. Conversely it is an agricultural waste which is available abundantly in tropical countries.25 ± 0. polyphenols.7 AAEg-1dw) assays. sandhaniya.28 ± 1. RHSK De Silva2 1 Institute of Indigenous Medicine. Future studies are warranted to determine the in vivo antioxidant and nephroproetcive activity in order to confirm the therapeutic potential of the leaf extract of A.89 ± 0. The results indicated that the aqueous leaf extract of A. kushtaghna and vishagna properties. antioxidative. moschatus in the management of chronic kidney disease Keywords: Abelmoschus moschatus. University of Kelaniya. its antimicrobial activity has not yet been investigated. The review was done through the available resources such as traditional text. Sri Lanka Spilanthes paniculata (Asteraceae) is commonly known as ‘acmella’ in Sinhala and ‘tooth ache plant’ in English. Phytochemical screening revealed 158 . ethanol and water extract of Capparis zeylanica leaves were showed antibacterial activity. Bacillus cereus. Sri Lanka Sarvavishadi Thaila (SVT) is one of the widely used herbal preparation in the traditional system of medicine in Sri Lanka which is consisting with many herbs. but none of the extract showed antifungal activity against Candida albicans and Aspergillus niger. WMB Weerasooriya2 1 Department of Cikitsa. Faculty of Applied Science University of Sri Jayewardenepura. spices and minerals which are having different therapeutic activities. Reviewing of the antibacterial. Gampaha Wickramarachchi Ayurveda Institute. Sri Lanka 3 Department of Zoology. chloroform. Pseudomonas aeruginosa and Salmonella typhi. However. It is highly utilized in traditional medicine to combat tooth aches. Sri Lanka 2 Department of Dravyaguna Vignana. University of Kelaniya. GM Rukmalee1. Faculty of Applied Science University of Sri Jayewardenepura. oils.coli. Sri Lanka 2 Department of Botany. journals and Science direct databases. Petroleum ether. Methanol and Petroleum ether extracts of rhizome of Curcuma zedoaria showed an anti-microbial activity against gram-positive. These studies reveal that the medicinal plants used in the formula of Sarvavishadi Thaila exhibit antibacterial and antifungal activity. The phytochemical screening was conducted using standard methods. Salmonella typhi. The present study aimed to investigate the in- vitro antimicrobial activity of ethanol. Keywords: Sarvavishadi Thaila.paniculata. Methanol extracts of leaves and stem-bark of Acronychia pedunculata exhibited good anti-Candial properties. HH Munasinghe2. NMMGSB Navaratne1 1 Department of Food Science & Technology. anti-bacterial activity. Klebsiella pneumonia. phytochemicals. The pod extracts (20mg/ml) were subjected to screening against both gram positive (Staphylococcus aureus) and gram negative (Escherichia coli) bacterial strains using standard protocol of Disc Diffusion Method (DDM) and Gentamicin (10 µg) was used as the positive control. Faculty of Applied Science University of Sri Jayewardenepura. LDC Peiris3. gram-negative bacteria and Fungi. anti-fungal activity PP 015: Phytochemical screening and antimicrobial activity of flower pod extracts from Spilanthes paniculata L. TGG Uthpala1.PP 014: Antimicrobial and phytochemical properties of medicinal plants used in Sarvavishadi Thaila RMW Liyanage1. petroleum ether and water extracts of the pods of S. Streptococus mutansand aqueous extract of garlic showed an antifungal effect against Trichophyton rubrum. antifungal effects of the medicinal plants used in SVT is the key objective in this study. The extract of Asparagus racemosus showed an excellent activity against candida species isolated from vaginal thrush patients and Aspergillus niger. The alkaloids from Adhatoda vasica have antibacterial activity against Staphylococcus aureus. Gampaha Wickramarachchi Ayurveda Institute. The aqueous and methanol extract of garlic has an antibacterial effect against E. All the crude extracts showed moderately high antioxidant activity for DPPH (0. antioxidant.8). endolichenic fungi.2 to 8.02 mg/mL) and FRAP (0. PA Paranagama 1 College of Chemical Sciences. The extracts exhibited antimicrobial activities with zones of inhibitions ranging from 8.86±0. Institute of Chemistry. Fungi can be grown using fermentation methods and solid cultures and need an insignificant amount of raw material. There are non-obligate fungi living inside the lichen thallus asymptomatically known as Endolichenic fungi. Further studies will be carried out to isolate bioactive compounds in fungus species Daldiniaeschoiltzii (PI/01). Sri Lanka 2 College of Chemical Sciences.3-0. The objective of the study is to determine antioxidant and anti-bacterial activities of 6 endolichenic fungi inhabiting mangrove lichens Pyxinecocoes and Opegraphaarabica in Sri Lanka. Keywords: anti-bacterial. Faculty of Science. extraction. 7. phytochemical screening. crude PI/01 (6. lichen 159 .the presence of tannin. petroleum ether and water extracts. alkaloids.05-0. which was comparable to the standard drug (22.8 and 14 to 18. PI/01/02 fraction showed the highest antioxidant activity (IC50=274. indicating it does not affect the biodiversity of the country.4 to 10.01 µg/mL).2 to 24. Sri Lanka and Department of Chemistry. In addition. Since antioxidant activity and antibacterial activity was high in PI/01 it was partitioned to obtain hexane (PI/01/01). The ability of the flower pod extracts of S.67 mg/mL) showed comparably high antibacterial activity against four bacteria strains Staphylococcus aureus.01 mg/mL) assays compared to standard BHT and Ascorbic acid. Bacillus subtilis and Staphylococcus epidermidis used in the agar well diffusion assay compared with the standard drug Azithromycin (5 mg/mL). antimicrobial activity PP 016: Antibacterial and antioxidant activities of endolichenic fungi isolated from mangrove lichens Pyxinecocoes and Opegraphaarabica in Sri Lanka PS Ishtaweera. water extract showed both higher effectivity against Staphylococcus aureus and Escherichia coli bacteria. quinone and total phenols in all three extracts while flavonoid was detected only in the ethanoic extract and saponins and gum mucilage were detected only in the water extract.8 mmrespectively for ethanol. Sri Lanka Lichen is a symbiotic association of fungi with algae or cyanobacteria. Institute of Chemistry. University of Kelaniya. The above fungi species were grown on 36 large Petri dishes using PDA as the medium and incubated at room temperature for 10 days and secondary metabolites were extracted into ethyl acetate. paniculata to inhibit the growth of both gram negative and positive bacteria is an indication of its antimicrobial potential which may be employed in the management of microbes in food contact surfaces. Escherichia coli. Among the extracts. chloroform (PI/01/02) and 60% methanol (PI/01/03) fractions. Keywords: Spilanthes paniculata. But. 30% of all deaths in 2010 were due to infectious diseases and the WHO forecasts 13 million deaths attributed to these causes in 2050. University of Sri Jayewardenepura. Faculty of Applied Sciences. Family: Rutaceae) is a medicinal plant found in Sri Lanka and the leaves. AM Abeysekera2. Streptococcus pyogens PP 018: Anti-inflammatory activity of the alkaloid fraction of Acronychia pedunculata leaves WMKM Ratnayake1. Tannins. Faculty of Applied Sciences. the 160 . Healthy adult male. University of Sri Jayewardenepura. Faculty of Medical Sciences.). Sri Lanka 2 College of Arts and Sciences. roots and fruits have been used for centuries in folk medicine for the treatment of various diseases. Keywords: papaya leaf. pedunculata leaves extract was evaluated. B Chandrasiri2. Escherichia coli.PP 017: Antibacterial properties of papaya (Carica papaya L. the anti-inflammatory activity of alkaloid fraction of A. Our previous studies have shown that 70% ethanol extract of A. TS Suresh1. Virgen Milagrosa University Foundation. N Salim3. Sri Lanka Acronychia pedunculata (“Ankenda” in Sinhala. G Abenilla2 1 College of Arts and Sciences.05) reduction of paw oedema formation when compared to negative control.w of indomethacin in 1 mL of 0. N Adeesha2. The results showed that both of EEAL and its alkaloid fraction showed significant (p<0. The 100% papaya Leaf extract showed the highest effect on Staphylococcus aureus and Escherichia coliwhile 25% leaf extract showed the lowest effect on Staphylococcus aureus and Escherichia coli. Results show that Papaya leaf extract has an antibacterial property against Streptococcus pyogens and Escherichia coli. Disc Diffusion method used to measure the Zones of Inhibition in crude extract concentrations ranging from 25- 100% while the positive control (Erythromycin) to streptococcus pyogens and (Ciproflaxacin) for Escherichia coli. Sri Lanka 2 Department of Chemistry. Virgen Milagrosa University Foundation.) leaf extract on Streptococcus pyogens and Escherichia coli.5% CMC. A parallel study could be conducted to add chemical formula which could enhance the antibacterial property of papaya (Carica papaya Linn. Our objective was to determine the active chemical constituents and antibacterial effect of Papaya (Carica papaya Linn.5% CMC respectively.0 mL of 0. Wistar rats weighing 150-200 g were used for the experiment. Philippines As highlighted by World Health Organization. Sri Lanka 3 Department of Botany. The negative and positive control groups were orally administered 1.05) anti-inflammatory activity on carrageenan induced rat paw oedema model. UG Chandrika1 1 Department of Biochemistry. Philippines and Center for Innovation in Biotechnology and Neuroscience.) leaf extract P Dissanayaka1.) leaf ethanol extract was used for the phytochemical tests. Phytochemical screening indicated that this plant is rich with alkaloids and in the present study.w of the 70% EEAL and its alkaloid fraction in 1 mL of 0. The test groups received 100 mg/kg b.5 % carboxymethyl cellulose (CMC) and 5mg/kg b. University of Sri Jayewardenepura. pedunculata leaves (EEAL) has significant (p <0. University of Sri Jayewardenepura. J Catanes2. anti-Bacterial. The active constituents Alkaloids. Unsaturated sterols and active Glycosides were present in the Papaya (Carica papaya Linn. Papaya (Carica papaya Linn. Streptococcus pyrogenes and Escherichia coli were cultured in Brain Heart Infusion (BHI) and Nutrient Agar culture mediums. stems.) leaf extract and to use the extract further as an alternative medicine to cure bacterial diseases. 14 ingredients were Herbal materials like Gammiris. Welmee.2% Snigdha.1%of ingredients contain Ushnaveerya which help in Vātānulomana and Kaphanissārana. Collected data were comparatively analyzed and demonstrated to identify the properties of different types of ingredients in selected formula.The aim of this study was to analyze the properties of herbal and mineral ingredients according to basic principles of Ayurveda. The properties were 61. Veerya.1% Lavanaand Kashaya rasa. This was a literature based study and the data was collected from indigenous classical text Vatikaprakaranaya.9% for indomethacin which is a positive control.4% for EEAL and 90.treatment with alkaloid fraction has activity enhancement when compared with the EEAL group. and 3 were minerals Sahindalunu. Sharkaradi Kalka is very effective in kaphajaroga like Kāsa. 61. Penelaeta. pedunculata. Walgammiris. According to data. Keywords: Sharkaradi kalka. used to prepare Sharkarādi Kalka. Considering the properties of ingredients including the grinding agent. Sri Lanka Sharkarādi Kalka is a medicinal preparation used for children in Ayurveda as well as in the Indigenous medicine of Sri Lanka. Walangasal. Tikshna and Ruksha helps in excreting vitiated Kapha. The maximum percentage inhibition of formation of rat paw oedema were found to be 81. They also help in reducing the thickness of Kapha. 61. anti-inflammatory.11. 33. Yawakshara. Suwandakottam etc. these observations provide valuable evidence for the anti- inflammatory properties of alkaloid fraction of leaves of A.1% Laghu and 50% Ruksha. JMPRK Jayasinghe Department of Dravyaguna. Keywords: Acronychia pedunculata.8% Tikshna and 22. According to the given data and Rasa. 18 ingredientswere identified. Suwasalunu. carrageenan PP 019: A descriptive study of Sharkaradi Kalka used in the Indigenous medicine practice of Sri Lanka KPMP Pathirana.3% Madura rasa.1% of ingredients are with Katuvipaka and helps in increasing Vāta. Katu and Tikta perform Vātavruddi and Kaphakshaya. University of Kelaniya. 38. Further studies will be undertaken to isolate the active compounds in this fraction. Gampaha Wickramarachchi Ayurveda Institute. In conclusion. Bees honey was used as the grinding agent. Guna. Swāsa in children as well as adults. Lagu increase the vāadosha and reduce the thickness of Kapha. Vipaka of the ingredients used in Sharkāradi Kalka it helps to reduce the thickness of vitiated Kapha and they are excreted by the increased Vātadosha.66.8% for alkaloid fraction at 5th hour while it was 45. properties 161 .6% of drauya contain Katu rasa and50% contain Tikta rasa. Medications used for this should be analyzed and case series should be done in future for confirmation of treatment regime. Different concentrations of the A. CT scan with biochemistry parameters.PP 020: Effects of Agni (digestive power) in management of cancer. The patient was referred to the oncologist and it was successfully removed. Centella asciatica juice. Lactate 162 . This is a case study of a 58 year old male. no appetite and severe malena. P Soysa Department of Biochemistry and Molecular Biology. PK Perera2 1 Ayurveda Research Institute. nil orally. A large tumour was observed in lesser curvature of the stomach extending towards cardia and surgery was deemed inappropriate and the patient was referred for Chemotherapy and Radiotherapy. and lifestyle related. In this study hepato-protective activity and reduction of oxidative stress of A. mercury etc. Faculty of Medicine. ascitis. Following the diagnosis of cancer. Treatment was continued up to six months and the patient was followed up with Endoscopy. Medicated kanji. garlic/milk decoction. From the second week. Atalantia ceylanica leaf extract is used in Sri Lankan traditional medicine for the treatment of different liver ailments.5-diphenyltetrazolium bromide tetrazolium reduction assay was used to evaluate the toxic concentrations of acetaminophen and A.5-Dimethylthiazol-2-yl]-2. Reactive metabolite of acetaminophen. Keywords: bowel carcinoma. ceylanica were studied. digestive power PP 021: Hepato-protective activity and reduction of oxidative stress by aqueous extract of Atalantia ceylanica on acetaminophen induced HepG2 human liver cell damage C Jayathilake. Sri Lanka 2 Institute of Indigenous Medicine. exploratory laporatomy was done to see the possibilities of excision. with a histopathologically confirmed diagnosis of adenocarcinama of the stomach with metastasis to the liver. special emphasis was given to restore Agni (digestive power) and hence. University of Colombo. were given in the treatment regime during the latter part of treatment. Sri Lanka Acetaminophen is the most frequent cause of drug induced liver damage in humans. In the management. According to Ayurveda. Sri Lanka Bowel carcinoma occurs at a high prevalence and risk factors identified are dietary. The patient was in a sub consciousness state with severe anaemia. Agni play a very important and vital role in maintaining good health. Aqueous extract of A. ceylanicaextract was co-exposed with acetaminophen (30 mM) which have a cell viability around 30% on HepG2 cells. Nutrition and Indigenous Medicine. After 4 months CT revealed that the tumour had narrowed down and localised. University of Colombo. traditional medicine. he did not have any sign and symptoms of malignancy. N-acetyl-p-benzoquinonemine causes hepatocyte injury. only juices of fruits and raw vegetables were given via nasogastric tube. ceylanica extract.A case study from the Ayurvedic perspective VPS Seneviratne1. generalised oedema. Fresh juices and decoctions of Āmapācana and Agni improving medicines were given according to the level of digestive power. ceylanica was prepared from tender leaves according to the procedure followed by Ayurveda. environmental. After 6 months. Ministry of Health. his condition gradually started to improve and appetite and bowel habits returned back to normal. suitable dietary modifications were applied. Rasāyana and Rasa medicines containing Gold. 3-[4. flowers and the whole plant were subjected to sequential extraction with hexane. stem.11 mg/ml. antilipidemic and antiamylase activities. ceylanica extract has hepato-protective activity and capability to reduce oxidative stress in acetaminophen induced HepG2 cell damage. ceylanica extract restored the reduced glutathione level towards that of untreated cells.9% relative to the acetaminophen treated cells respectively. roots. dichloromethane and methanol after continuous stirring for 24 hours. The plant materials were collected from Jaele. several extracts showed activity similar to Orlistat which is a drug used currently to treat obesity (+ve control). dichloromethane. Hence. oxidative stress. hepatoprotective. P Paranagama2. acetaminophen PP 022: Evaluation of functional activity of Wrightia antidysenterica. dichloromethane flower extract. and methanol stem extract showed inhibition similar to Orlistat (43%) at 1 mg/ml concentrations as follows respectively 39%. Faculty of Science. 39%. Sri Lanka 2 Department of Chemistry. Wrightia antidysenterica is an endemic plant species in Sri Lanka. and 38%. 39%. the results of the current study illustrate the presence of antioxidant and antilipidemic activity in Wrightia antidysenterica extracts. antilipidemic 163 .An endemic plant species AI Kuruppu1.47 mg/ml. ceylanica at a concentration of 80 µg/mL with values of 64. 40%. Significant reduction (p<0. R De Silva1 1 Interdisciplinary Center for Innovations in Biotechnology & Neurosciences. No toxicity was found with the plant extract alone on HepG2 cells (EC50>3500 µg/mL). Sri Lanka. University of Kelaniya.8% and 68. Hexane. Results were compared to Butylatedhydroxytoluene (+ve control) which showed a mean IC50 value of 0. University of Sri Jayewardenepura. The antiamylase assay did not show activity with any of the Wrightia antidysenterica extracts. We found that the methanol stem extract demonstrated the highest anti-oxidant activity with a mean IC50 value of 0. Keywords: natural products. In the antilipidemic assay. gamma-glutamyltransferase (GGT) leakage and reduced glutathione (GSH) content were determined after 24 hours incubation. A. We evaluated the activity of extracts of Wrightia antidysenterica for their antioxidant. Finely powered leaves. A. Antioxidant activities of these extracts were evaluated using DPPH and FRAP assays while antilipidemic and antidiabetic activities were investigated using lipase and amylase enzymes. Highest reduction of LDH and GGT leakage were observed for A. antioxidant.05) in percentage leakage of LDH and GGT were observed in the cells co-exposed with A. Further activity of these extracts tested using various bio-assays and chemical compounds will be isolated and characterized. Sri Lanka The search for novel pharmacological agents for various non-communicable diseases has grained increasing demand with the rising number of illnesses. Keywords: Atalantia ceylanica.dehydrogenase (LDH) leakage. ceylanica aqueous extract and acetaminophen compared to the acetaminophen treated cells. methanol extracts obtained from the whole plant. Faculty of Medical Sciences. Plant materials still contain the best active compounds to be used as drugs for various diseases.26 respectively. 40. Sri Lanka Evaluation of the cytotoxicity of compounds is an essential part of drug discovery. tannins. General Sir John Kotelawala Defense University. SU Kankanamge1. General Sir John Kotelawala Defense University. which has not been previously studied.05). saponins. But little is known about the toxicity of most of these plant materials. The concentration series of 2. using methanol as the blank. terpenoids.05 mgmL-1 of leaf extract and a solution of 2. University of Peradeniya. University of Kelaniya. 36. 1. in vitro cytotoxicity of a serial dilution concentration series from 50 mg/ml of five Sri Lankan medicinal plants were evaluated using MTT assay on non-cancerous monkey kidney (vero 76) cell line. 0. Air- dried. sterols. Faculty of Allied Health Sciences. glycosides and reducing sugars were qualitatively observed. Faculty of Veterinary Medicine and Animal Science.31 and 34. Leaves were collected from the Horana area. UV-B. a positive statistically not significant relationship was observed in between SPF and concentration (r=0. Sri Lanka 2 Department of Basic Sciences. Sri Lanka 2 Department of Kaumarabruthya and Stree Roga.97. In this study.63.5. 13. RPVJ Rajapakse1 1 Department of Veterinary Pathobiology. 0. Alkaloids.93. phenols. The absorbance of each sample was determined in triplicate by spectrophotometry in the range of 290–320 nm.25. The SPF values were calculated using the Mansur- equation.8% methanol. the recent trend is to search for human-friendly herbal sunscreens. The plants used in this study are 164 . According to Pearson’s correlation. at 5 nm intervals. 42. The filtrate was evaporated to dryness and subjected to preliminary phytochemical analysis. RN Pathirana2. HP Wakkumbura2.0 mgmL-1 commercially available sunscreen-Dermatone® (Labeled SPF-35) were prepared in methanol. 0. photo-protective. and 0. Since the presence of profound sun screening activity was found. The SPF of leaf extract with respect to the concentrations 2. 0. KJK Karunathilake1. With the realization of adverse effects.PP 023: Determination of in-vitro photo-protective potential of Methanolic Leaf extract of x Citrofortunella microcarpa (Calamondin) AAMDDN Amarathunga1. This study focused on the in-vitro evaluation of Sun Protection Factor (SPF) of leaf extract of x Citrofortunella microcarpa: Calamondin (Family: Rutaceae). Sun Protection Factor. 0. p>0.25. Sri Lanka Synthetic sunscreens have been introduced as a preventive strategy for the harmful effects of ultraviolet radiation on humans. powdered plant material was macerated for 4 days in 99.0. NA Sanjeewani1. KNK Ranasinghe1. WD Ratnasooriya2 1 Department of Pharmacy. Gamapaha Wickramarachchi Ayurveda Institute. Faculty of Allied Health Sciences. this would offer an exciting avenue for further research towards the development of herbal sunscreens of high importance especially for the people living in tropical countries. 1.0.5. Therefore evaluation of cytotoxicity is a must before conducting drug experiments.655. Keywords: Calamondin. flavonoids.38.0. x Citrofortunella microcarpa PP 024: Evaluation of the potential in-vitro cytotoxic effect of some Sri Lankan medicinal plants on Vero 76 cells WMD Wickramaarachchi1.0.05 mgmL-1 and Dermatone® were 43. Faculty of Medicine.79.46.49.79. 60.12.12. Curcuma longa and Stereospermum suaveolens.94 & 79. Only 50mg/ml extract of Glycyrrhiza glabra showed a significant toxic level compared to positive control. University of Ruhuna. Therefore.54. sub lethal dosage.90. 54. Mean cTnI concentrations of groups 1-9 were 0.77.05 is considered significant. Faculty of Medicine. A significant difference (P<0.05) between group 1 and groups 2-9 was evident in all three diagnostic parameters. Toxicity exhibited in cardiomyocytes is related to free radical formation. Sri Lanka 2 Department of Biochemistry. 16. Overall. 63.06. MTT assay was carried out to determine the cytotoxicity with non-treated cells as negative and ethanol as positive controls respectively. Coscinium fenestratum. 58.92. The objective of this study was to determine the sub lethal dosage of doxorubicin to screen selected medicinal plants for cardio-protective activity. Sri Lanka 3 Department of Pathology.07.83U/L and SOD activities were 77. Cytotoxicity. Keywords: doxorubicin.01. mean AST activities were 38. RP Hewawasam2.54pg/mL respectively. University of Ruhuna. p<0. LKB Mudduwa3 1 Faculty of Medicine. 197. after 16 hour fast). Degree of histopathological changes increased with increasing dosage and damage was more pronounced in the sub-endocardial region.05.92. 14.It can be concluded that 25 mg/ml of Glycyrrhiza glabra and 50 mg/ml of the other four plants can be used safely in drug preparation without any cytotoxic effect. 116. 18.23. 39. 28. Animals were sacrificed on the 4th day and blood was collected for the estimation of cardiac troponin I (cTnI). MTT assay. biochemical and histopathological results indicated 18 mg/kg doxorubicin as the most appropriate sub lethal dosage to be used in future studies. 50. 57. Ayurveda PP 025: Determination of the sub lethal dosage of doxorubicin for the screening of selected medicinal plants for cardio-protective activity JAN Sandamali1. ip. 87. 66. It exhibits a cumulative dose related cardiotoxic profile that limits its clinical utility. 147. 15.Glycyrrhiza glabra.93. Sri Lanka Doxorubicin is highly effective against a spectrum of malignancies. After 24 hours. Cyperus rotundus. 42. 21. AST and SOD activities and heart tissues were collected for histopathological assessment. medicinal plants with high antioxidant activity have the potential to combat doxorubicin induced cardiotoxicity. 14.34 & 8. 72. 163. Groups 2-9: eight doses of doxorubicin (13. 19 & 20mg/kg. The results were statistically analyzed using GraphPad Prism 7. For this. 16. 44.0 with one-way analysis of variance.73.57. Keywords: medicinal plants. cells were seeded in 96 well plates and after 24 hours incubation. cardiotoxicity 165 . In groups 1-7. Results showed that almost all the concentrations of each plant extract had no toxicity compared to control with p<0. Group 1: normal control.96. 68.75%. treated with different concentrations of aqueous plant extracts.38 & 221.96. KAPW Jayatilaka2. Wistar rats were divided into nine groups of 10 animals in each. 17. University of Ruhuna. Fra did not significantly impair paw oedema.PP 026: Activity-guided fractionation of aqueous fraction of acetone extracts of Pleurotus ostreatus in the search of anti-inflammatory agents WJABN Jayasuriya1. anti-inflammatory activity.5 % and 55. Pakistan Pleurotus ostreatus (P. aqueous fraction. Treatment with fractions Frb. Our previous work investigated Serotonin-producing enterochromaffin (EC) 166 . column. University of Sri Jayewardenepura. 2nd. Frc and Frd were selected for further fractionations. University of Sri Jayewardenepura. However. C Meedeniya1 1 Interdisciplinary Centre for Innovation in Biotechnology & Neuroscience. Frc and Frd showed inhibition of paw oedema at the 1st. Therefore. Sri Lanka 4 Department of Biochemistry. Sri Lanka 3 Department of Chemistry. Frb. University of Sri Jayewardenepura. Sri Lanka An increasing health burden and emphasis on health and well-being has driven a global resurgence of alternative health care strategies.o remain to be elucidated. MI Choudhary5 1 Department of Allied Health Sciences. S Pilapitiya2. GH Fernando2. The Frc and Frd showed 52. The traditional medical practices of Sri Lanka. oxidative burst PP 027: Fundamental neuroscience research and traditional medicine: investigating the Gut. This study evaluates the anti-inflammatory activity directed fractionation of the aqueous fraction of acetone extract (AqFrA) in order to identify the fraction having the highest activity.2 % of inhibition on whole blood oxidative burst. Sri Lanka 5 International Center for Chemical and Biological Sciences.7 % (5th hr). TS Suresh4. University of Colombo. chromatography. divided into 8 major zones. DTU Abeytunga3.8 % (4th hr) and 83. Frc and Frd showed significant inhibition of rat paw oedema. R De Silva1. Keywords: Pleurotus ostreatus.o and its fractions. Activity guided fractionation resulted in the fractions with higher anti-inflammatory activity than the acetone extract itself. University of Karachi.o) is a culinary-medicinal mushroom grown worldwide. The AqFrA was purified using size exclusion chromatography and four fractions (Fra. Sri Lanka 2 Gampaha Wickramarachchi Ayurveda Institute. CA Wanigatunga2. Anti- inflammatory activity of each fraction was measured using carrageenan induced rat paw oedema model and the effect on reactive oxidative burst of human whole blood was measured by luminol enhanced chemiluminescence activity. respectively. We have reported the anti-inflammatory potential of acetone extract (AE) of P. Frc and Frd) were obtained. 4th and 5th hour when compared with the Control. A spectrum of physiological processes is modulated by the neuro-endocrine substance Serotonin. Sri Lanka 2 Department of Pharmacology. A principal therapeutic approach formulating multiple physiological and patho- physiological processes targets the close association of the digestive and central nervous system. the active anti-inflammatory constituents in AqFrA of P. with synchronized“therapies” modulating multiple physiological systems.2.Brain Axis K Wanigathunga1. University of Sri Jayewardenepura. promotes a holistic approach to health. is considered fundamental to human wellbeing. where the gastrointestinal tract (GIT). Frc and Frd showed maximum inhibition of oedema of 77. It is possible that the main active compounds might be hydrophilic as the activity retained in polar AqFrA. and the mechanisms validated in disease models using DNA probes. University of Kelaniya. S Pilapitiya2. traditional medicines. with seminal data on serotonin release kinetics1. showing the recruitment of the newly born cells into lesioned brain regions1. Gut-Brain-Axis PP 028: Fundamental neuroscience research and traditional medicine: Accessing the central nervous system K Wanigathunga1. University of Sri Jayewardenepura. We now investigate Arma Parchana. Sri Lanka 2 Gampaha Wickramarachchi Ayurveda Institute. The treatment of neurological disorders remains a challenge in allopathic medicine as therapeutic access to the central nervous system (CNS) is impeded by the Blood Brain Barrier (BBB). neuro-genesis and maturation. C Meedeniya1 1 Interdisciplinary Centre for Innovation in Biotechnology & Neuroscience. assessed using clinical criteria. focusing on human neurological disorders. Indigenous and Ayurvedic therapies of Sri Lanka however use novel approaches to treat CNS and related disorders. Keywords: neurological disorders. in functionally discrete regions of the GIT was shown. Virechana. blood and clinical assessment criteria. traditional medicines. Our collaborative research program will use traditional therapies of human neurological disorders.cells in the mucosal epithelium of the GIT.2. towards rationalizing novel treatment strategies. Sri Lanka The incidence of neurodegenerative disorders are positively correlated to the increasing age of the global population. Regional variation in EC cell density. Our “Nasya” and related treatments evokes CNS neuro-genesis. neuroscience 167 . Keywords: neurological disorders. Disease models using DNA probes. mediating improved CNS function. using microbiological. We previously used intranasal (Nasya) and related treatments including manipulation of the nervous system to induce neuro-genesis deep within the brain. supporting our experimentation on intranasal and related treatments used in Sri-Lankan Ayurvedic and Indigenous medicine. Agni Deepana and Vasti karma“conditioning” treatments of traditional medicine. R De Silva1. markers of apoptosis and of serotonin synthesis will be investigated. Embedded neuroscience and traditional medical expertise of our research collaboration investigates the “Serotonergic regulation of the gut-brain axis. Now we test the hypothesis that traditional Nasya and related therapies evoke neuro-genesis and other physiological brain responses. markers of neuro-degeneration. including “Nasya therapies” as a doorway to the brain. Sri Lanka Non-communicable diseases (NCDs). that it incorporates physical. 31. 76. Western Province. physical activities. Psychological comfort reasons given toward some remedies such as. PKK Madushika. parental and family influence [91. YS Deegodagamage. A stratified. cut injury [56. WMCS Wijesundara. are a major cause of morbidity and mortality globally and is one of the greatest development challenges of the 21st Century.8% (20/205) rural.5% (116/205)].PP 029: Perception of medical undergraduates and pre-intern doctors of University of Sri Jayewardenepura on traditional beliefs and home remedies for common illnesses DHM Niluka.3% (177/205)]. Faculty of Medical Sciences.1%(109/205)]. Keywords: home remedies.4% (165/205)]. Data regarding demography and perceptions on Traditional beliefs and home remedies for common illnesses were collected and analyzed using SPSS v-16. Most were aware of home remedies for common illnesses.4% (48/205). The main purpose of this study is to analyse the situation of lifestyle towards the increasing trend of NCD risk factors in a major part of the Sri Lankan population and to decrease morbidity and disability associated with NCDs by changing lifestyle through Ayurveda knowledge. Gastritis [54. From 205 participants. Our aim is to assess the perception of medical undergraduates and pre-intern doctors of University of Sri Jayewardenepura (USJP) on Traditional beliefs and home remedies for common illnesses. is used by those who fear the discomfort of medical treatments [80. Medical undergraduates and pre-intern doctors of USJP who have used home remedies for preventing or treating illness have a positive attitude towards them. Dietary habits. Doctors should have a sufficient knowledge regarding home remedies. PH Dissanayake.7% (188/205)]. Populations were 58.8% (133/205)]. LA Indunil. beliefs. Sri Lanka Traditional beliefs and home remedies have been in practice from ancient times. medical undergraduates PP 30: An impact of Ayurveda knowledge on prevention and control of major non-communicable diseases in the labour force in Sri Lanka IDKD Dissanayake Provincial Department of Ayurveda.6% (196/205)]. to recommend positive remedies.7% (65/205) urban and 9. Future studies should focus on the reliability of home remedies for continuous practice by the doctors. SG Yasawardene Department of Anatomy. mental and spiritual aspects of health [55. EAST Edirisinghe.1% (111/205)]. The majority positively believed that home remedies. contain less side effects [57. use of alcohol and tobacco and 168 . heart disease and diabetes) patients of age 15-60 years was selected randomly. The practice of home remedies had a positive influence on environmental factors like own knowledge [76. 23. the belief that using them are not harmful [75.5% (120/205) semi-urban. A self- administered questionnaire was distributed among 205 participants.1% (156/205)]. to discourage harmful practices and also to have a good doctor-patient relationship. Common cold/Cough [95. Gastroenteritis [64.1% (113/205)]. friends’ influence [60.1% (154/205)].6% (157/205) were males and females respectively. University of Sri Jayewardenepura. which are largely preventable by changing lifestyle.5% (118/205)] and build up the body’s own defenses and promotes self-healing [53.4% (124/205)] and prior teaching [86. probability sample of 327 NCD (stroke. n=327).241. Keywords: adult population. W Weerapana.135. diabetic patients are at risk of developing complications such as diabetic nephropathy and diabetic wounds. the quality of life of the all four patients were improved and the diabetic wounds were completely healed. Nawinna Sri Lanka Prevalence of Diabetes mellitus in Sri Lanka is high (8%). Ayurveda lifestyle based knowledge on dietary habits (r=0. case II was a diabetic patient with nephropathy. The main 169 . WJ Wickramarachchi Gampaha Wickramarachchi Ayurveda Institute. They all were advised on appropriate diet during the treatment course. diabetic wounds PP 032: A survey of the most frequently used medicine for Prameha (Diabetes Mellitus) NVPS Madhushani. lifestyle PP 31: Clinical experiences in the geriatric clinic with special reference to diabetes mellitus and its complications C Welivitegoda. p=0.24 months. SG Kisholorjan.financial condition were assessed by using a standardized questionnaire based on the Ayurveda knowledge of Charaka and Sushrutha Sanhitha. Diabetes mellitus. The purpose of the study was to assess the clinical efficacy of indigenous medical care for diabetes and its complications. Following the courses of treatment for 3. p=0.000. One of the male patients was a retired medical practitioner and had a diabetic wound for 3 months and the other one was a laborer with diabetic nephropathy awaiting for dialysis. even after skin grafting. A convenient sample of four cases were retrospectively studied in the geriatric clinic at the Bandaranaike Memorial Ayurvedic Research Institute.310. p=0. one of them had the complication of the unhealed diabetic wound for 12 months. diabetic nephropathy. n=327). Because of poor care and poor disease education. and randomized clinical trials are recommended.004.60 years.based materials and mineral origin materials.000. BADH Kularathna Bandaranaike Memorial Ayurveda Research Institute. Dependent variable and independent variables have a statistically significant linear relationship. Case I was a diabetic patient without complication.160. Nawinna. physical activities (r=0. Keywords: Non-communicable diseases. According to the investigation.015. MUM Perera. Sri Lanka The Ayurveda system of medicine has exclusive treatment for the management of diabetes. This Cikitsa includes a wide range of herbal. It is recommended that an extensive clinical study be performed to monitorAyurvedic based behavioral patterns of patients who suffer from NCDs and to monitor for a considerable period of time in order tosupport the presentation of Ayurveda Knowledge Based Lifestyle to the society. The patient with diabetic nephropathy had a serum creatinine level that came close to normal and was able to refrain from dialysis. n=327) of the people were highly related with prevention and control of NCDs respectively. The researchers suggest that diabetes with different complications could be managed successfully with Indigenous medicine. use of alcohol and tobacco (r=0. Ayurveda knowledge. cases III & IV patients had diabetic ulcers. The four patients were in the age range of 55. p=0. Both diabetic females were house-wives. risk factors. This was a study of case series. n=327) and also financial condition (r=0. 61). Ten medicinal plants were selected for the analysis according to their Panchapadarthas. their proportions in relative use. Aralu. There are various treatment approaches for hemiplegia in Sri Lanka. Sri Lanka The prevalence of stroke in Sri Lanka is much higher compared to other South Asian countries. Sheethaveerya (60%) were prominent. Faculty of Medicine.03) whereas physical function (p=0. Data was collected by interviewer administered questionnaire. Among three Vipakas. Among twenty Guna's the highest recorded were for Lagu (47%) and Ruksha(47%) equally. the most recorded was Madura (60%). minerals. In the study groups. Rajagiriya and 45 hemiplegic patients who attended the physiotherapy department of the Neurology unit. Faculty of Medicine. According to Ayurveda literature. The purpose of this study was to assess the quality of life of the patients who receive Ayurvedic management and patients who receive physiotherapy management for hemiplegia after a stroke. their Panchapadartha. chemical constituents and relevant Sahapanaand Anupana in the management of Prameha (Diabetics). The chemical composition of selected medicinal plants considerably varied. their Panchapadartha. The collected data were analyzed according to the type of medicinal plants. Keywords: Prameha. chemical constituents and use of Sahapanaand Anupana. Medical Outcome Short Form 36 questionnaire 170 . Keywords: stroke. General health perception improved significantly with Ayurvedic treatment (p=0. Shilajathu PP 033: Quality of life of patients receiving Ayurvedic treatment or physiotherapy for hemiplegia following a stroke DMT Nishadika1. Ayurvedic treatment. physiotherapy. minerals. Sri Lanka 2 Department of Pharmacology. both Ayurvedic treatment and physiotherapy approaches improved most aspects of quality of life of the hemiplegic patients. Stroke may cause hemiplegia and this affects the quality of life of patients. The study was a descriptive cross sectional design using 45 hemiplegic patients who attended the Ayurvedic hospital. Thirty registered Ayurvedic practitioners in Kaluthara District were selected in this study and data were collected through a questionnaire and interviews. bee honey is the most frequently used Sahapanand Anupana(70%). Among the three Veeryas. quality of life. both groups of hemiplegic patients studied had similar improvement in overall quality of life by receiving either Ayurvedic treatment or physiotherapy (p=0. K de Abrew2 1 Department of Allied Health Sciences. National Hospital Sri Lanka within 6-18 months after a stroke. The study also comprised of extensive review of Vruddhathraya and other traditional medicine books. hemiplegia. A convenience sampling method was used.001) improved significantly with physiotherapy. The study revealed that Araluis is the most commonly used medicinal plant while Shilajatu was found to be the most frequently used mineral (52%).04) and mental health (p<0. University of Colombo. When the quality of life of two groups were assessed by the Short Form 36 questionnaire. liquefied and detoxified Kapa and Medodhathu and clear the Srothas.objective of this study was to identify the most frequently used medicinal plants. Outcome measures were determined by a socio demographic questionnaire and Medical Outcome Short Form 36 questionnaire. According to Rasa Guna the selected drugs facilitate absorption. The analysis revealed that out of six rasa's. the predominant rasa was Kasaya (32%). University of Colombo. It comprised of root extracts of Withania somnifera.41%. this study aims to review the applicability of different dosage forms.2% became normozoospermic. research on the effects of these preparations are scarce. Therefore. Sri Lanka 3 Nugegoda Ayurvedic Private Clinic.65) years and 31.94) years respectively.8% of men both progressive motility and viability were abnormal. Keywords: traditional herbal medicine. where nearly one-third of children are affected. Faculty of Medical Sciences.PP 034: Sperm parameters of men seeking traditional Ayurvedic treatment for infertility DHR Nawagamuwa1.56 (3. Assessment of effects on sperm parameters at least six months after treatment with the herbal preparation and assessing pregnancy outcomes are recommended for future studies. RDR Lakmini1. Pre and post treatment means of sperm parameters were compared with paired t test. Improvement in mean (SE) of sperm count [10. asthenozoospermia was reduced by 25. Before treatment. The tested herbal preparation was given in powder form continuously for a period of three months. After treatment. Sri Lanka Sri Lanka has inherited a splendid history of the indigenous medical system. The tested herbal preparation was given in a powder form. Sri Lanka Although herbal preparations are used in the treatment of male infertility. Orchis mascula.35(3. Furthermore. Mucuna pruriens. 22. motility and viability increasing the number of normozoospermic men after treatment. remain a serious problem in Sri Lanka. Seminal fluid analysis reports were obtained before treatment.05). University of Sri Jayewardenepura. All men seeking treatment were pathozoospermic and the most prevalent abnormality was asthenozoospermia (84. Sri Lanka 2 Department of Physiology. The aim of this study was to determine the effects of a traditional herbal preparation on sperm parameters of men seeking infertility treatment.32) %] were significant (p<0. RHSK De Silva. a paediatric disorder. progressive motility [14. Mean duration of infertility was 50. HGSG Wijesiriwardhana Department of Desheeya Chikitsa. in 96. doses and their Anupāna used for 18 types of Māndam diseases. Demographic data of men (n=91) seeking treatment at an Ayurvedic infertility center were recorded after informed consent. Mean (SD) age of male and female partner was 34. Institute of Indigenous Medicine.30(2. RARP Susantha3 1 Department of Allied Health Sciences. sperm parameters PP 035: Different dosage forms of Anupana used in Sri Lankan traditional medicine in Mandam Roga (Malnutrition) Chikitsa. Sperm parameters were taken at monthly intervals for three months after treatment with the herbal preparation.86) vs 26. Māndama (malnutrition).84(2.82(2.04) vs 24. University of Sri Jayewardenepura. male infertility. Classification of semen abnormalities were done according to WHO guidelines.32) vs 18. University of Colombo. In conclusion the herbal preparation tested significantly improved the sperm count. Data is gathered from the series of texts Talpate Piliyam a collection of 171 .18 months (approximately 4 years) and 96.1%). DMS Fernando2. It has several disciplines which were descended from the hierarchy of medical practitioners and Māndam Vedakama (treatment for malnutrition) is one of them.38(4.75(2. Faculty of Medical Sciences.65) %] and viability [14.A Critique RLDS Ranasinghe.7% had primary infertility. Tribulus terrestris and Asparagus racemosus.75(2.79)106/ml]. traditional medicine treatment PP 036: Genetic diversity of Santalum album L. album L. As such. using traditional medicines Keywords: Māndam disease. Māndam disease can be effectively managed. ISSR markers 172 . Therefore. antibacterial and antiviral properties. was optimized which identified that the bark tissues give the highest quality and quantity of DNA. 16 primers resulted in polymorphic bands. Kaṣāya (8%) and Cūrna (5%) are given to possess Agni Dīpana (72%). Having continuous supply of uniform raw materials is a key for sustainable industrial applications. Kāyam (8%). Guli (25%). Guli are administered with Anupāna. genetic diversity. Here we described preliminary work assessing the genetic diversity of sandalwood in Sri Lanka using ISSR markers. University of Peradeniya. The most common dose of Cūrna is Bidāla Mātrā. Moreover. is famous for its medicine.27%. In MāndamCikitsā. In conclusion. The indication of different doses may be due to the age factor. Kashaya should be quarterly reduced (4:1) and the dose is 1/4 Pata (60ml). based on Sodium Dodecyl Sulphate (SDS). sandalwood oil is widely used for development of nutraceuticals due to its antispasmodic. S. PCG Bandaranayake Agricultural Biotechnology Centre. identification of superior mother plants is critical. as one plant per area. Hisa gelvum (5%) and Vedu (5%) are the commonly used external applications. Total number of alleles was 76 and polymorphic percentage was 77. comprise of the highest alpha and beta santalene content in its oil. Virecana (14%) and Vamana (12%) actions in the body. indicating higher genetic diversity of a given population. breast milk is the most used among the identified 40 types. Rucikāraka (65%). Of those 23 produced reproducible bands. DNA extracted from the bark tissue was assessed with 37 ISSR primers. aroma and victual properties. Samples were collected from the 10-15 year old trees grown in home gardens. Since the grower has to wait around 15-20 years for harvesting. The DNA extraction protocol.Basna (7%) and Talapa (3%) are nutritional supplements to establish Vṛṁhana (32%) and Tarpana (23%) properties. selecting superior genotypes with high commercial value would be important for industrial sustainability. While micro-propagation would be an option for mass production of identified mother plants.Ola leaf transcripts. in Sri Lanka. Three major growing areas of the country. Faculty of Agriculture. nutraceutical. Sri Lanka Santalum album L. Kalka (18%). Thaila is given as both Pāna (8%) and Abhyaṅga (7%).A preliminary study KMAN Kulathunga. malnutrition. was used. Ten different dosages of Guli (pills) were identified which are explained in the sizes of the fruits. selecting of a genetically superior genotype for it biochemical properties among this genetically diverse population is the proper way to attain its industrial sustainability Keywords: sandalwood oil.. according to the age and the type. Among the eight commercially valuable species of Santalum genus. commonly known as sandalwood. Substitution threats were blending with cassia and away from quill processing. common export destinations. DAM De Silva Department of Agribusiness Management. with few players. In the study. where 8 focus groups. Mahailluppallama. callus is used as an intermediate stage in plant regeneration and friable callus is a type of callus. high entry and exist barriers were key determinants of the competitor rivalry. 76.24% for exporter.7% for pharmaceutical industry. medicinal and ornamental values. knowledge and market intelligence. DMJB Senanayake1. limited processes and is generally simple. Peelers gain 33. WAR Dhammika1. limited value addition. lack of quality organic fertilizer. The barriers for organic certifications were cost. which easily falls apart and is used for the generation of cell suspension cultures. value chain PP 038: Induction of friable callus in chilli variety California Wander (Capsicum annuum L. Organic cinnamon value chain is short. This study was carried out at the tissue culture division of the Field Crops Research and Development Institute. Sri Lanka 3 Department of Botany. Product typology identified 3 main types. A mixed method approach equipped for value chain analysis. Development of tissue culture protocols for chilli is important since it is necessary for use in plant genetic improvement and other studies. None of the producers were in the certified category.9% for cosmetics.5% of organic exporters were certified. Availability of numerous competitors. Sri Lanka 2 Department of Plant Sciences. Sri Lanka Chilli (Capsicum annuum L. WAM Harindra. Price premiums were marginal for the upstream of the value chain while downstream enjoyed attractive premiums for the organic value added products. slow industry growth. has 4 major market segments. cotyledon and hypocotyl 173 . 40% for spice and seasoning and 9. insignificant brand loyalty. WMW Weerakoon1. 2.8% for bakery and confectioneries. KASI Kumari1 1 Field Crops Research and Development Institute. University of Colombo. WTPSK Senarath3. protracted procedures and heavy documentation. certified organic (international) and conventional organic.76% for grower and 89. Experience. 50.3% of profit share. The profit share among each value chain node for C grade is 10. certified organic (local). Faculty of Agricultural Sciences. switching costs are entry barriers. Mahailluppallama to understand the friable callus induction in chilli under three different tissue culture media. University of Sri Jayewardenepura. Keywords: organic cinnamon. In tissue culture. Sabaragamuwa University of Sri Lanka The main purpose of this study is to identify the attitude towards organic farming through price premiums.) is grown throughout the world and well known for its culinary.) PJK Dassanayake1. GAU Jayasekara2. similar size of market share. consumer preferences and the perception of cinnamon producers. Chilli shows a highly recalcitrant response to tissue culture experiments and it is believed to be due to the presence of capsaicinoids and their phenolic intermediates.PP 037: Organic cinnamon: an innovation in the Ceylon cinnamon value chain WMS Warnakulasooriya. 90 interviewer administered questionnaires for value chain actors and 100 web based analysis offend markets were instrumental to collect the data. Mexico. The main export market. undifferentiated products. H Gunasekara2. as assessed by Kirby Bauer test. Ethyl acetate (EA) extracts of bark sample (1 g in 10 ml. V Suriyakumara1.4-D) (1mgL-1. Keywords: Alpha amyrin. anti-inflammatory and anti . N Wijekoon1. contained five major essential oils with alpha amyrin accounting for 13. TLC-DPPH assay. Keywords: chilli. clinical 174 . This study brings insight to support the pharmaceutical value of G. EA and methanol fractions of an endophytic Fusarium species isolated from bark also showed antimicrobial activity against the same test organisms and additionally against E. R de Silva1 1 Interdisciplinary Centre for Innovation in Biotechnology and Neuroscience. This is in fact. dry weight basis. a triterpene which is reported to possess antimicrobial.1% while that of the host contained 2. University of Sri Jayewardenepura. and host bark tissue also showed antioxidant activity in a preliminary qualitative spot test in TLC. Amyrin. The EA fraction of Fusarium sp. coli.7%. callus induction percentage PP 039: Agarwood. The medicinal value of this plant is overlooked although it had been used for centuries in traditional medicine. Parkinson's Disease (PD) has long been associated with cognitive impairment which has been found to correlate with age and food habits. 2mgL-1. There was no significant difference for callus induction among three media. University of Sri Jayewardenepura. Methicillin–Resistant Staphylcoccus aureaus (a clinical isolate) and S. antimicrobials PP 040: Effect of tea consumption on cognitive decline in Parkinson’s Disease Y Imran1. Sri Lanka Gyrinops walla (Gaertner) is one of the species that produces agarwood resin with tremendous importance in the perfume industry. beyond perfumery: In-vitro antimicrobial properties of Gyrinops walla and one of its endophytic fungus. L Gonawala1. Fusarium sp. aureus ATCC 25923 on Muller Hinton Agar and Candida glabrata (ATCC 90030) on MHA+ 2% glucose respectively. walla as a plant source of it. Further studies are underway to isolate amyrin from the fungus to confirm its antimicrobial activity. N Salim Department of Botany. Furthermore. Fusarium sp. These results reveal that hypocotyl ex- plants are more suitable for callus induction in chilli. tissue culture. Sociodemographic. Gyrenops walla. Hypocotyl. The results showed that the callus induction percentage within 28 days was significantly higher with hypocotyl explants when compared to the use of cotyledons. Cotyledon. walla and its associated endophytic microorganisms with potential antimicrobial and antioxidant properties under in vitro conditions.explants from in-vitro germinated two weeks old seedlings were used for callus induction in MS medium containing different concentrations of 2.4-Dichlorophenoxyacetic acid (2. P Dissanayaka1. Sri Lanka As reported in literature. extracted thrice) showed antimicrobial activity against pathogenic bacteria.DPPH assay.tumor activities is notably a plant resin. H Somarathne. a new report on fungal-derived alpha amyrin as well as G. 3mgL-1). The GC-MS analysis revealed that EA fraction of Fusarium sp. Sri Lanka 2 Sri Jayewardenepura General Hospital. This may be because of the high dissociation energy of MgCl2 than NaCl. Keywords: Parkinson’s disease. Therefore future studies should be conducted such as measurement of ionic conductivity. electro chemical test and direct current polarization test. The Fourier transform infrared (FTIR) spectroscopy was used to identify the ion-ion interactions for all the salt added jack tree latex.0001). Results indicate a general trend that consumption of tea (>3cups/day) may have a positive effect on reduction of the progression of cognitive decline in PD. verbal fluency sub scores 1cup/day vs 2 cups/day (p=0. These results showed that a clear influence on the vibrational modes of the polymer after addition of salt. Sri Lanka Polymer electrolytes have been identified as the next materials to develop in the energy storage systems due its availability. 64±8 Yrs. when it is the same percentage on NaCl and MgCl2. jack tree latex. The relationship between dietary patters and ACE-R subscale scores were evaluated. the main objective is to investigate jack tree latex as a natural polymer instead of synthetic polymers in order to develop commercial batteries.0001). Number of tea cups vs sub scores of ACE-R. MgCl2 always produces higher absorption peaks than NaCl. ACE-r. language sub scores 2 cups/day vs ≥3 cups/day (p=0.0001. Keywords: polymer electrolyte. Male. 40%). The study was done in synthesis and characterization of gel polymer electrolyte based on jack tree latex by incorporating mostly abundant alkali ions such as Na+. HMJC Pitawala Department of Science and Technology. memory sub scores 1 cups/day vs 2 cups/day vs ≥3 cups/day (p=0. age range 44-85 Yrs. so cation interaction is high for MgCl2. Uva Wellassa University. Synthetic polymers are used in batteries. Female. With the FTIR results. cognitive decline PP 041: Synthesis and characterization of gel polymer electrolyte based on jack tree latex A Karthigan. Results may pave the way towards further evaluation of the effect of tea consumption as a modifying treatment/ healthy beverage for cognitive decline in PD leading to innovative product development.016). Therefore.Revised (ACE-R) respectively. PD patients. In the present study PC acts as plasticizers to improve the physical properties and morphology of the electrolyte.data. tea. 60%. level of cognitive decline of clinically diagnosed PD patients (n=32) evaluated using a standard questionnaire and Sinhala version of Addenbrook’s Cognitive Examination. battery 175 . orientation/attention 2 cups/day vs ≥3 cups/day p= 0. Propylene carbonate (PC) added sodium chlorate (NaClO4) was also incorporated with jack tree latex in a ratio of 1:10. Mg2+ as NaCl salt and MgCl2 salt respectively. Interestingly having ≥3cups/day may have a positive effect on the other sub score of ACE-R for PD. consumption of at least 2 cups/day may have a positive effect on the verbal fluency sub score of ACE-R for PD. visuospatial abilities sub scores 2 cups/day vs 3 cups/day (p=0.011). Specifically. Onset.0001). Number of tea cups/day vs total scores of ACE-R. 2cups/day vs ≥3 cups/day (p=0. (n=32. they are expensive and caused health issues. The FTIR result was used to investigate interactions between polymer host and salt. 11). Rosaceae etc consist of a variety of phyto chemicals. antispasmodic and anti- inflammatory properties. 200 Ayurveda practitioners (age 30-52yrs) participated in 4 ultra USS knowledge sharing workshops at Colombo and Gampaha during 2013-2015. De Soysa Maternity Hospital.65 (SD+/- 2. flavoring drink and food. bark and roots. The aim of this article is to emphasize the medical importance of essential oil. it is very important to upgrade the day to day knowledge about modern medical investigations. absolute oil extraction. but these workshops had a significant positive impact on immediate knowledge gain. The mean pre-test score was 13. It is vital to enhance the knowledge on ultrasound scanning (USS) related investigation issues among healthcare Ayurveda medical practitioners. Eucalyptus essential oil has been used for antiseptic. The main objective of this study is to evaluate the impact of knowledge sharing workshops on basic USS investigations.000 MHz. Ultrasound scanning is a simple imaging technique which uses high frequency sound waves higher than 20. V Shiyamala 1 Unit of Siddha Medicine. workshops. such as medicinal uses. This oil is distributed in different parts of the plants including flowers. Essential oils in these plants contain concentrated hydrophobic liquid containing volatile aroma compounds. Essential oil has important therapeutic actions such as antioxidant. 176 . Eastern University of Sri Lanka 2 Teaching Hospital of Siddha Medicine. A survey method was adopted as the research methodology of this study and pre-test and post-test surveys were conducted using a self- administrated 20 question questionnaire among workshop participants. Ayurveda practitioners PP 043: Role of essential oil in medical therapy S Janani. anti-bacterial. peels. Plants belonging to families like Labiatae. muscular stress. digestive disorders and nervousness. antispasmodic and anti-inflammatory conditions. abdominal disorder. leaves. Trincomalee. Essential oil is generally extracted by distillation. skin. Peppermint essential oil is used for mouth freshener. antimicrobial. Some oils are inhaled while some are applied to the skin and some are consumed internally for health benefits. Essential oils are used for many purposes. Sandal wood essential oil is used to treat mental disorders. were included as the research samples.57) and post-test score was 17.09 (SD+/-2. Keywords: ultrasound scanning. Phyto compounds are responsible for the curative effect in herbal medicine. The knowledge on USS investigation among Ayurveda health care professionals in Sri Lanka was suboptimal. applications and patient preparation knowledge in Ayurveda medical practitioners. Sri Lanka Indigenous medical therapy is popular globally because it uses side effect free herbal products. Thus. Trincomalee Campus.PP 042: Impact of workshops on ultra sound scanning related knowledge in Ayurveda medical professionals WHAP Senanayake Radiology Department. and household cleaning products. The results were analyzed using SPSS version 18. resin tapping and cold pressing. Sri Lanka As modern medical technology improves rapidly with global development. often by using steam and other processes including solvent extraction. the knowledge sharing workshops have a positive impact on improving medical practice. N Varnakulendran. increased concentration and memory. cosmetics. Increasing sustainability and add value to the foods claiming health benefits is a key improvement in food industry. dried hibiscus powder has incorporated with selected two Ceylon black tea brands (X and Y) to increase health benefit of black teawhile increasing popularity of hibiscus among people. The method identified by the research is suitable for development of a composite spice oleoresins flavoring in coconut oil. Keywords: essential oil. KAA Piyumal. Spicy oil is an edible oil product which incorporates flavor and aroma compounds of six commonly used spices (chili. The Spicy oil was highly preferred by a semi-trained sensory panel. a composite spice oleoresins flavoring in coconut oil SPL Galappaththi. HKVS Nimalsiri. In this study. Hence incorporating these extracts into coconut oil will enhance its health effects. spices. 25% hibiscus incorporated black tea was selected as best combination in the sensory evaluation from 25%. ODAN Perera Department of Food Science and Nutrition. clove. Faculty of Livestock. HN Fernando. garlic.Total anthocyanin 177 .37. University of Sri Jayewardenepura.Color. to the refined.For both brands. bleached.Total phenolic content. Fisheries and Nutrition. Laboratory experimented shelf life of spicy oil was about 6 months according to the determined physical. onion. KDSCN Rajapaksa. and deodorized coconut oil or virgin coconut oil. moisture content. solvent extraction. PMS Shashikala Department of Food Science and Technology. phytochemicals PP 044: Development of “Spicy oil”. Keywords: cooking oil. which enhances immunity and is an excellent remedy for skin. total anthocyanin content and total flavonoids content were determined under functional properties. which is a convenient product. shelf life PP 045: Functional and physicochemical properties of hibiscus incorporated black tea RTK Premathilaka. AE Amarasinghe. The extracted compounds from the six different spices and the coconut oil were mixed together to the most preferred and accepted ratio by 32 members of a semi–trained sensory panel in order to make a composite spice and cooking oil mixture. ash content and pH were determined under physicochemical properties. This solvent extraction method extracts phytochemicals from the spices (according to the literature) and these phytochemicals give many health benefits. Coconut oil is a commodity which cannot be neglected in cooking. Sri Lankan cuisine. Sri Lanka Sri Lanka is a tropical country where almost all the spices are grown rich and naturally. Both spices and coconut oil have health benefits. HGE Wijesooriya. clearing scars and blemishes. UMW Dahanayake. such as lowering cholesterol and blood pressure. cosmetics. Faculty of Applied Sciences. 50% and control. chemical and sensory properties. cinnamon and cardamom) in Sri Lanka. A solvent extraction method was used to extract flavor and aroma compounds. raising HDL cholesterol and has anti-inflammatory effects etc. Wayamba University of Sri Lanka The flower of Hibiscus rosasinensisis an indigenous edible flower in Sri Lanka.5%.Lemon oil is an antioxidant. or sometimes reddish pumpkin with thin skin and deep vertical lobes. In this study. KB Wijesekara. Sol-gel approach was used to synthesize products using diluted Nitric acid under different conditions.53. Hydroxyapatite. University of Sri Jayewardenepura. LDAM Arawwawala3 1 864 C.21 respectively. The study concludes that there is a possibility of producing hydroxyapatite using commercially available Eppawala chloroapatite in Sri Lanka. Sri Lanka Eppawala chloroapatite deposit is the only commercially available phosphate source in Sri Lanka. Sri Lanka The fruits of Garcinia cambogia (G. greenish. surface morphology and micro-structural features.18±9. while using as a natural colorant. X-ray powder Diffraction (XRD). bonding type.33±3. Synthesized Eppawala hydroxyapatite powder was characterized using X-ray Fluorescence (XRF). Bioceramics PP 047: A comparison of phytochemical and physicochemical parameters of Garcina cambogia and Garcinia zeylanica AU Hewageegana1. Sri Lanka 2 Department of Science & Technology.30±4. This results indicate the potential of exploiting this flowers as a source of developing novel functional beverage. 329. HMJC Pitawala. Fourier-transform Infrared Spectroscopy (FTIR). Robert Gunawardhana Mw.33 and 115. respectively.41±1. RD Gunaratne. Uva Wellassa University. 41. presence of functional groups. crystallinity. look like a small yellowish. It is a bioceramic which can be used for applications in orthopedics and dentistry due to its structural similarity with the mineral phase of mammalian bones and teeth. PP 046: Synthesis and characterization of hydroxyapatite from Eppawala Rock Phosphate for biomedical applications as a value added product HKGKDK Hapuhinna. zeylanica) (belonging to family: Clusiaceae). EMDK Ekanayake 1 Department of Biosystems. Malabe. and Scanning Electron Microscopy (SEM) in order to find out its composition. University of Colombo. Sri Lanka 2 Institute of Indigenous Medicine. Keywords: Eppawala chloroapatite. The XRD results reflected the formation of a hexagonal crystal structure of hydroxyapatite.00. As a conclusion Total Anthocyanin content was increased in hibiscus incorporated black tea than black tea alone. The FTIR spectroscopy results confirmed the formation of hydroxyapatite from apatite via the presence of hydroxyl groups. Those results coincided with the FTIR results of human and cow bones. The colour can vary considerably. it was chemically changed into hydroxyapatite as a value-added product.content (mg/100g of fresh matter) for infusion of 25% hibiscus incorporated X black tea/Y black tea and X/Y black tea alone were 461. which is situated in Anuradhapura district. Elementary composition and micro-structural features of products were discussed based on the XRF and SEM results of the synthesized hydroxyapatite powder. HGSP Hewageegana2. When the rinds are dried and cured in 178 . Sri Lanka 3 Industrial Technology Institute. cambogia) and Garcinia zeylanica (G. Phenols.73. The present attempted to compare the phytochemical parameters in the two plants.19. cambogia water extract was 1. pH in G.57.79. 0. Twenty patients with Kitibha (psoriasis) were treated with Weliwenna Virecana. TLC PP 048: Liver and kidney toxicity in Weliwenna (Dimorphocalyx glabellus) Virecana (purgative action) in the treatment of Kitibha (psoriasis). There were slight differences in Rf values between G.05 (at 25 o C) whilst in G. Physicochemical properties. 0.12.preparation for storage and extraction. 0. for liver function and urea and creatinine levels for kidney function. 0. No significant difference was observed in blood urea levels or creatinine levels before and after treatment with Weliwenna Virecanacan and the values are in the reference range.54.70± 0. Specific gravity of both fruit extracts was 1. Results of the study were statistically evaluated using Minitab 18. 0. zeylanica in terms of phytochemical and physicochemical parameters. It is also used in the treatment of skin diseases especially in Kitibha (psoriasis). 0. Virecana. 0. 0. Institute of Indigenous Medicine. Evaluation of phytochemical screening. Faculty of Medicine. There were similarities and as well as differences between G. The dried rinds are used in traditional recipes for cooking in many Southeast Asian countries.30. flavonoids. alanine aminotransferase (ALT).01 (at 25 oC). The decoction for Virechana was prepared by mixing 5 g of powdered fresh root bark of Weliwenna with 120 ml of boiling water. 0.07. Keywords: Kitibha. steroids and terpenoids were present in higher amounts in both G.15) in the TLC fingerprint profile.A case series A Devanarayana1.05 were considered as significant for all experiments. P values < 0. University of Colombo. Keywords: Garcinia cambogia. 0. The liver function tests were within the normal range before the treatment except for two patients.04. zeylanica (254 nm: 0. 0. Phytochemicals. ERHSS Ediriweera2.60. 0. cambogia and G.94 and 366 nm: 0. Garcinia zeylanica.67 and 366 nm: 0. Weliwenna.73 ± 0. psoriasis. Tamil name: Tentukki) is a plant used in Virecana Karma in Sri Lankan traditional medicine.07 ± 0. The values were decreased in all patients including the two patients who showed higher values after the treatment with Weliwenna. 0. 0.47. zeylanica. Sri Lanka 3 Department of Molecular Biology and Biochemistry. Serum samples were analyzed for aspartate aminotransferase (AST). cambogia and G. Sri Lanka 2 Department of Kayachikithsa. Our study shows that Weliwenna Virecana therapy can be employed to treat Kitibha (psoriasis) disease without any damage to the liver or kidney. 0. Total ash. water soluble ash and acid insoluble ash contents were higher in G. 0.14. University of Colombo. 0.66) and G.19. 0. P Soysa3 1 National Ayurveda Teaching Hospital.04. toxicity 179 .32. zeylanica it was 1. cambogia fruits than that of G. 0.42. 0.23. The filtered decoction was administered orally. physicochemical parameters and development of Thin Layer Chromatography (TLC) fingerprint were carried out for both fruits. alkaloids.48. The Wilcoxon’s signed-rank test was used to compare the treatment effects. they are dark brown or black in colour. cambogia (254 nm: 0. saponins.06. The present study was carried out to determine the biochemical changes in Waliwenna Virecana therapy. Blood samples were collected before and at the end of the treatment (48 hour) of Virecana. Sinhala name: Weliwenna.11. lactate dehydrogenase (LDH). zeylanica extracts. Sri Lanka Dimorphocalyx glabellus (Family: Euphorbiaceae.15 (at 25 oC). 0.08. crude lipids as 1. B Senevirathna3.. except in the NO scavenging activity assay. DPPH. Sri Lanka 3 Institute of Postharvest Technology. Glycyrrhiza glabra. textural properties. Tephrosia purpurea and Vernonia cinerea was formulated by an expert panel of Ayurvedic physicians at Link Natural Product (Pvt) limited. Trolox the standard showed 97% scavenging activity at 1 μg/ml whereas LLC showed 55% scavenging activity at 1000 μg/ml. This indicates that anti-oxidant effects do not play an important role in the reported hepatoprotective activity of LLC. Osbekia octandra. The antioxidant capacity of LLC was evaluated via total phenol content (Folin–Ciocalteu method). Eclipta alba.1-diphenyl-2-picrylhydrazyl (DPPH). University of Sri Jayewardenepura. NO scavenging activity PP 050: Edible gel from Gracilaria salicornia for health food application GDP Somasiri1. The gel hardness of the gel solution (1. Moreover. P. The extracted agar yield.125 - 2 μg/ml) and LLC (50. ABTS. T. Sri Lanka 2 Hayleys AquaAgri (Pvt.36 ± 0. Sri Lanka 3 Department of Pathology. Hayleys Agriculture Holdings. total phenols. Quercetin (25 -400 μg/ ml) and the extract of LLC (50 -1000 μg/ ml) showed a dose dependent elevation in NO scavenging activity. National Aquatic Resources Research and Development Agency. S Thiruchenduran3 1 Department of Food Science & Technology. while total carbohydrate was determined from the Dubois method. Gracilaria salicornia contained crude protein as 8. The objective is to develop food gels from Gracilaria salicornia as red seaweed is currently under-utilized in Sri Lanka. bellerica. ABTS cation scavenging activity of trolox (0. and infra-red (FT-IR) spectrum were investigated and the results were compared with laboratory-grade agar. I Wickramasinghe1. Phyllanthus amarus. UG Chandrika2.) Ltd. w/v) was 180 . The gallic acid equivalent phenolic content of the LLC was 1050/7g.PP 049: Antioxidant Activity Of Linklive CareTM: A hepatoprotective polyherbal formulation KAAU Karunarathna1. At the same concentration the extract of LLC showed 50 % NO scavenging activity respectively. The proximate composition was determined by AOAC methods. Sri Lanka Seaweed has been utilized in traditional medicine for centuries. Tinospora codifolia. Faculty of Applied Sciences. I Wijesekara1.5%. Sri Lanka 2 Biochemistry.2-azinobis-ethylbenz-thiazoline-6-sulphonic acid (ABTS) and Nitric oxide (NO) scavenging activity. Keywords: hepatoprotective formulation.67 ± 0. Boerrhavia diffusa. AM Abeysekera1 1 Depatment of Chemistry. University of Sri Jayewardenepura. K Sivaram2. Sri Lanka Link LivecareTM (LLC): a new hepatoprotective polyherbal formulation that consists of 14 herbs: Andrographis paniculata. University of Sri Jayewardenepura.7367 ± 0. Gacilaria salicornia was collected from Valaipadu beach in the Northern Province. Terminalia chebula. respectively. DPPH scavenging activity of the extract of LLC (10 -300 μg/ml) and gallic acid (2 -10 μg/ml) exhibited dose dependent activity with IC50 values of 50 μg/ ml and 5 μg/ ml. 1.24%. Quercetin the positive control reached 58 % at 400 μg/ ml.1000 μg/ml) also showed dose dependent scavenging activity. emblica.11%.12% and considerably high amounts of ash as minerals 27. These results show that the antioxidant capacity of the extract of LLC is low compared to the standards. 2. the antioxidant activities of dried seaweed powder were screened. Curcuma longa. Agar gel was extracted by hot water extraction. C Padumadasa1. University of Sri Jayewardenepura. Piper longum. 50 μg/mL for free linamarin. Medical Research Institute. Sri Lanka Nanoparticulate delivery of nutraceuticals provides a new insight in prevention and therapy for cancer. Keywords: seaweeds.667± 1. health foods. GAJ Amaratunga3. In this study the in vitro cytotoxicity of linamarin loaded chitosan nanoparticles were evaluated using MCF-7 cells.5% gel solution) was 4. Colombo.5 g. it was delivered and internalized more effectively than free linamarin. University of Peradeniya. thermo gravimetric analysis (TGA). 48 and 72 hours. Sri Lanka 2 Department of Chemistry.7±127 g. mineral and low total lipid content. gels. Cell viability was decreased to about 40-50% after 48 h and to about 24-38% after 72 h with the nanoparticles. DN Karunaratne2. The gel viscosity at 80̊C (1.155CP. Linamarin. while the commercial agar tested was 5926. In vitro cytotoxicity results revealed that when linamarin was loaded into nanoparticles. nanoparticles. Synthesized nanoparticles were around 50 nm with higher positive zeta potential and higher encapsulation efficiencies (85%). The FT-IR spectrum is almost similar with commercial agar. Sri Lanka 4 Institute of Bio Chemistry. University of Colombo.577 CP. The seaweed examined in this work has appreciable protein. Molecular Biology and Bio technology. The morphological observations obtained by inverted microscopy were in good agreement with those results. In free linamarin. Time dependent cytotoxicity was highlighted than dose dependent cytotoxicity in linamarin loaded chitosan nanoparticles. linamarin. SR Samarakoon4 1 Department of Natural Products Chemistry. FT-IR. MCF-7 cells 181 . Linamarin loaded chitosan nanoparticles were obtained by ionic gelation between chitosan and sodium tripolyphosphste and characterized using dynamic light scattering. The successful loading was confirmed by FT-IR and TGA results.7 ± 46. Gracilaria salicornia. The cytotoxicity of free linamarin and linamarin loaded chitosan nanoparticles were assessed by SRB assay.48 μg/mL while it was 373. The IC50 value after 72 h for linamarin loaded chitosan nanoparticles was 26. Cells were exposed to different concentrations of linamarin loaded into chitosan nanoparticles and free linamarin for 24.551. Sri Lanka 3 Sri Lanka Institute of Nanotechnology.05) and lower than the commercial agar gel. nutraceuticals. TEM and SEM techniques. and it can be used as ingredients for health foods including food gels for diabetics. while the commercial agar was 14. The gel strength and viscosity of the yielded gels were significantly different (p≤0.667 ± 0. Keywords: chitosan. V Karunaratne2. nutraceuticals PP 051: In-vitro cytotoxicity of Linamarin encapsulated chitosan nanoparticles against human breast cancer cells IHV Nicholas1. found in cassava has proven as a drug candidate for cancer treatment due to its cyanogenic property. Faculty of Science. 50% inhibition of cells was not achieved even after 72 h. and Sargassum muticum from the Nothern coast. Proximate chemical compositions were compared in these two varieties.62 ± 0. Sri Lanka This study was conducted to evaluate the possibility of using three underutilized sea weed species found in Sri Lankan coastal areas. University of Sri Jayawardenepura. crude protein (23.PP 052: Evaluation of the potential antioxidant properties and proximate chemical compositions of Indian and Ethiopian black cumin (Nigella sativa) RCN Thilakarathna. Jaffna.17 ± 0. Keywords: nigella sativa. DPPH radical scavenging assay: IC50 value of 8. The antioxidant capacities of ethanol extracts of the two varieties were preliminary determined for the presence of antioxidants by testing reducing power and following quantification by DPPH radical-scavenging activity and ABTS assay. Sri Lanka This study was carried out to evaluate the possibility of using Indian and Ethiopian varieties of black cumin for the food and pharmaceutical industries by analyzing their phytochemical and proximate compositions. cleaned and were oven dried at 60 ̊C for 6-7 hours.34mg and 7. Indian and Ethiopian varieties.22± 0.70%) were slightly higher than the quantities of Indian variety and fat (37. Sargassum. antioxida PP 053: Evaluation of the potential antioxidant properties of three varieties of sea weed collected from Sri Lankan coastal areas SD Rukunayaka.31 mg and 6. ABTS radical scavenging assay: IC50 value of 9.84 ppm) whereas the lowest was recorded in Caulerpa racemosa. total phenolics 182 . Both Ethiopian and Indian varieties of black cumin could be identified as a great potential to be used in food and pharmaceutical industries because of its high antioxidant capacity and both are potential sources of dietary fibre and fat. Sargassum crassifolium and Caulerpa racemosa from the south western coast.27 mg of Gallic acid equivalents per mL. Sargassum muticum and Caulerpa racemose. (DPPH radical scavenging assay: IC50 value of 0.35 mg of GAE per mL & the TPC of 23.13%) and total carbohydrate (32.9 mg of Gallic acid equivalents per mL respectively. Sargassum muticum shows the greatest potential to be used in food and pharmaceutical industries because of its high antioxidant capacity and it is best utilized in fresh form. fiber(6. Faculty of Applied Science. antioxidant. Sargassum crassifolium. were manually collected. SB Nawarathna Department of Food Science and Technology.11%). Hikkaduwa. Caulerpa.82 ± 1.53 ppm) All three varieties showed higher antioxidant capacity in fresh form than in dried form. The highest antioxidant capacity and total phenolics were observed in Sargassum muticum (DPPH radical scavenging assay: IC50 value of 3. for the food and pharmaceutical industries by analyzing their antioxidant capacity and total phenolic content. Faculty of Applied Sciences.90%) contents were slightly higher in the Ethiopian variety. The antioxidant potential of the three varieties were determined both in dried and fresh form after being extracted into methanol by evaluating DPPH radical-scavenging activity and ABTS assay.98 ± 0.03%). University of Sri Jayewardanepura. RAUJ Marapana Departmennt of Food Science and Technology.62 mg of GAE per mL & the TPC of 16. Proximate analysis of Indian Nigella seeds showed that moisture content (8.30± 1.29± 0. Keywords: seaweeds.49 ± 0. Higher antioxidant capacity was observed in the Ethiopian variety than in the Indian variety. Faculty of Allied Health Sciences. aggrana and rasana pareeksha may apply with some limitations. pavonina showed desired sunscreen activity. Sri Lanka and identified from the National Herbarium of Sri Lanka. Data collection was conducted using a self-administrated 20 questions questionnaire among participants and the results were analyzed using SPSS version 18.0.99.54. darshana.25. 0. General Sir John Kotelawala Defense University. The concentration series of 2. The absorbance of each sample was determined in triplicate by spectrophotometry in the range of 290–320 nm. Keywords: Adenanthera pavonina. there is an essential need of introducing modern technology (MT) to the AHCS in the process of Globalization. The filtrate was evaporated to dryness and subjected to phytochemical analysis. 0. Air-dried. and 0. The SPF of leaf extracts with respect to the concentrations 2. locally known as “Madatiya”. Faculty of Allied Health Sciences. a positive statistically not significant relationship was observed between SPF and concentration (r=0. 0.61. But today with more physical development than mental. 14.05 mg/mL of leaf extract and a solution of 2. SPF values were calculated using Mansur equation. p<0. is a medicinally valuable plant used to treat a variety of diseases in traditional medicine. 1.5. Ancient rishis of Ayurveda use super mental power.0.PP 054: Determination of in vitro sun protection factor of methanolic leaf extract of Adenanthera pavonina NA Sanjeewani1. According to Pearson’s correlation. Leaves were collected from Aranayake. shravana.56. 1. Since extract of A.09 and 34. Our AHCS has a large number of different traditional ways to diagnose and cure diseases. A survey method was adopted as the research methodology and 100 AHC professionals (age 30-70 years) and 100 patients (age 30-70 years) at Colombo and Gampaha during 2013-2015 were included as the research samples. Sri Lanka 2 Department of Basic Sciences. sparshana.25. RN Pathirana2. Phytochemical analysis revealed that the extract of A. Sun Protection Factor. this easily available herb can be used for development of a safe and potential sunscreen cream for an affordable price. The objective of this study was to identify lack of knowledge as a challenge and barrier of introducing MT to AHCS. Sri Lanka The Ayurvedic Health Care System (AHCS) is popular among Sri Lankans as well as gaining popularity in the modern world. at 5 nm intervals. methanolic extract. 3.26.05). 35. General Sir John Kotelawala Defense University.0.0 mg/mL Dermatone® were prepared in methanol. Therefore. They use the third eye to diagnose. WD Rathnasooriya2 1 Department of Pharmacy. pavonina. sunscreen PP 055: The issues and challenges of introducing modern technology to the Ayurvedic Health Care System WHAP Senanayake Gampaha Wickramarachchi Ayurveda Institute. respectively. University of Kelaniya. The objective of this study was to evaluate the in vitro sun protection factor (SPF) of leaves of A. pavonina contains alkaloids.05 mg/mL and Dermatone® were 36. 0.0.9619. Sri Lanka Adenenthera pavonina (Family: Fabaceae). using methanol as the blank. Lack of 183 . 26. 0.5. powdered plant material was sonicated for 20 minutes in distilled methanol. G Wijayaratne2. Sri Lanka 2 Department of Microbiology. Ras Al Khaimah. Keywords: biogenic. characterization and antimicrobial activity studies of biogenic silver nanoparticles from Garcinia cambogia M Napagoda1. lack of government supports and investments due to poorly-skilled policy makers of the upper ranks of AHCS (48%). Faculty of Medicine. Although silver nanoparticles appear as promising candidates against microbial resistance. Ayurveda health care system. the present study is undertaken to synthesize silver nano-particles from fruits of Garcinia cambogia. cambogia were treated with silver nitrate and the development of nano-particles was monitored using UV-visible spectrophotometer. a common plant in Sri Lanka. and lack of monitoring of improvements of the few modern technologies which apply to AHCIs (98%). Garcinia cambogia. patients. United Arab Emirates The emergence of multi-drug resistant micro-organisms is a global problem that demands the development of alternative antimicrobial remedies. the nano-preparations were screened for antimicrobial activity against Staphylococcus aureus. Staphylococcus saprophyticus and nine strains of Methicillin- Resistant Staphylococcus aureus by broth micro-dilution assay. Higher Colleges of Technology. Methicillin-Resistant Staphylococcus aureus. Hexane and dichloromethane extracts of G. lack of knowledge about introducing MT to AHCS among Ayurveda health care professionals. the conventional physio-chemical methods involved in the synthesis are potentially harmful to the environment. University of Ruhuna. the biogenic synthesis of silver nanoparticles has been actively pursued as an environmentally benign approach. The minimum inhibitory concentration values were observed in the range of 31. Detailed studies on antimicrobial and disinfectant properties and the mode of action of synthesized silver nano-particles are in progress. University of Ruhuna.25 -125 µg/mL against the tested microorganisms.knowledge about modern technology applications among AHC Professionals (37%) and patients (57%) was shown. western medical professionals having less knowledge about AHCS (76%). The change in the initial colour of the reaction mixture and the high absorbance between 220-240 and 440-460 nm indicated the reduction of silver ions into elemental silver. S Witharana3 1 Department of Biochemistry. and to evaluate the antimicrobial and disinfectant properties of the nano-preparations. The morphology of the synthesized nano-particles was confirmed through scanning electron microscopy as clusters in the range of 200 nm. Allopathic medical professionals and the policy makers are significant issues and barriers. knowledge PP 056: Synthesis. These preliminary findings provide a solid platform towards the development of eco-friendly silver nano-formulation with potent disinfectant properties. silver nano- particles 184 . Therefore. Faculty of Medicine. Sri Lanka 3 Faculty of Engineering. S De Soyza1. In conclusion. Keywords: modern technology. As a consequence. Subsequently. Challenges identified were. 2. Medline and Medlar etc. Meditation gives neurological changes of the body.531 [95% CI: 0. Total PSS score of Pre/Post intervention were statistically analyzed. improve breathing. reduce blood pressure. Trincomalee campus. were consulted to fulfill this review. Sri Lanka It is reported that stress could possibly increase the metastatic spread of cancer. meditation improves immune system. T Somasundaram2 1 World Class University Project. They were four Randomized Control Trials with a total of 196 participants. The reputed siddha text and modern text were reviewed as well as the Journals from Elsevier. It enhances telomerase activity lead to secrete higher levels of melatonin and serotonin. and depression. Konesapuri. anxiety stress. Keywords: addanga Yoga. energy level. Transcendental meditation gives. pineal gland PP 058: A critical review of assessing the correlation between prognostic values in breast cancer patients and Yoga based stress reduction S Yogeswaran1.Physiological changes and health benefits of Meditation (Thiyanam) S Varnakulendran1. loneliness. Pubmed. in studies met with inclusion criteria.003)] and revealed evidence of 185 . decision making and problem solving. Google scholar and Cochrane Library through October 2017. increased cardiac output and cerebral blood flow and apparent cessation of carbon dioxide in the body. Evidence was found for short-term effects on perceived stress [Standardized Mean Difference = 1. V Shiyamala2 1 Siddhamedicine. This review focuses on critically assessing the correlation between the outcomes of prognostic values in breast cancer patients and stress reduction on Yoga based therapeutic interventions. better cognitive skills and creativity. Trincomalee. fibromyalgia. enhance self-esteem and self-acceptance improve mood and emotional intelligence. Evidence suggests Yoga has been utilized as a therapeutic intervention to reduce stress in cancer patients. and lengthen longevity etc. meditation.509. Sri Lanka 2 Institute of Post-harvest Technology. Physically. the Thiyanam or meditation is seventh step which is presents in a manner of verse in Thirumanthiram by Thirumoolar. prevent arthritis. Database searches were carried out in PubMed. MC Riyas2. Scopus. fear. Among the eight limbs. p= 0. Psychologically it enhance emotional wellbeing such as reduce worries. an integrated response with peripheral circulation and metabolic changes.PP 057: A Review. Meditation is a scientific art for resting mind and attaining complete control over the mind. It is concluded that proper yogic meditation induces kundalani and ignite electro-thermal induction (Moolaperuneruppu) via Ventrothalamic tract (Veenathandu) and secrete amuthu (Nectar) from pineal gland which nourishes energy of mind and body by its physiological function. There are many scientific studies were conducted on the benefits of meditation. Meditation gives a boost for mind such as increasing mental strength and memory. Sri Lanka Both Pathanjali and Thirumolar are expert siddhars in attanga Yoga. R de Silva1. 4 out of 8 articles were screened based on perceived stress level measured during or post-treatment using Perceived Stress Scale (PSS). Eastern university of Sri Lanka 2 Teaching Hospital of Siddha medicine.552]. The aim of the review was to expedite the physiology and its effect of mental and physical health. University of Sri Jayewardenepura. National Aquatic Resources Research and Development Agency. Ayurvedic doctors performed both activities. AMHS Attanayake Bandaranaike Memorial Ayurveda Research Institute. Sri Lanka also has own traditional medical system. raw drugs. This research statistically discusses the challenges of dispensing Ayurvedic drugs. Sri Lanka Traditional medicine can mentioned as medicinal system which has been developed over generations by using indigenous knowledge. Dispensing is one fold of Chathurpatha. metastatic spread. The pilot study was based on 30 consumers. In the ancient time however. Currently. Meta-analysis failed to demonstrate the effect of Yoga on metastatic spread and recurrence of breast cancer. MW Kalyani2 1 Ayurvedic hospital. The research tool is a structured questionnaire with the dependent variable of consumer satisfaction and independent variables such as dosage forms. dispensing environment. Variables are reviewed in the literature. based on the population of the Puttalam district. Kirimetiyana. WDCK Karunarathna. University of Sri Jayewardenepura. Results showed that external factors do not influence Ayurvedic stakeholders as they depend extremely on outsiders. Analysis was with external factors and internal factors. over the counter deliveries and simultaneous usage of western and eastern drugs. The main focus of this study is drug deliveries. Successful treatment depends on four folds of Chathurpatha. Ayurvedic central dispensaries. The analytical tool used was SPSS 22. advertisement. thus it warrants a long term trial of systematic and scientific evaluation. Keywords: Chathurpatha. Sri Lanka 2 Department of Business Administration. Dispensing means drug production and deliveries. brands. drug manufacturing units perform drug production and pharmacies take over drug deliveries. Factor analysis was with 383 samples. Ayurvedic private practitioner’s places and private Ayurvedic pharmacies. stress reduction PP 059: A study of challenges in dispensing ayurvedic drugs with special reference to Puttulam district in Sri Lanka K Sivadharan1. over the counter deliveries and simultaneous usage of western and eastern drugs. However. none of the above studies assessed the correlation between stress reduction and metastatic spread or recurrence rate of cancer. free Ayurvedic dispensaries. packaging. This paper. handling by skilled people. is designed with the Ayurvedic dispensing places. The sampling method depends on stratified random sampling. namely Ayurvedic hospitals.efficacy only for Yoga in stress reduction during active cancer treatment. 186 . Ayurvedic pharmacies face upward and downward developments. Sri Lanka This research paper discusses the major challenges of dispensing Ayurvedic drugs. consumer satisfaction PP 060: A study on the common practice of selected traditional guli kalka used in out patient deapratmet in Ayurveda hospitsls – A liatreure survey MGDN Balasooriya. Keywords: Yoga. The major challenges encountered are wrong advertisement. Ayurvedic dispensing. Kalka and Thaila can be found in ancient books. Therefore. Chandra Kalka. 49% mentioned that the activity remains upto two hours and 39% mentioned that the activity remains upto three hours. 18% has mentioned that the activity persist only for the first hour. According to the results it can be concluded that by changing the Anupana single drug can be used to treat several diseases. The specialties of these drugs are they can be used to treat several diseases by changing its Anupana. Keywords: traditional medicine. Seetharama Vati and Chandra Kalka can use to treat vomiting and diarrhoea in the absence of Nawaratna Kalka. Over 50% formulas found in Seetharama Vati for vomiting. repeated application in every two hours can avoid mosquito bites in children between the ages 1-4 years to a greater extent without any complications. Seetharama Vati and Suranvidura Vati for stomache pain. Chandra Kalka. Collected data were tabulated and percentages were analyzed for the activity response on the first. traditional Guli Kalka. Southern Province. Four diseases also selected and all the Anupana was referred to gather data and were categorized according to the disease type. Suranvidura Vati. Chandra kalka. a formulation was developed based on western baby oil formulation and the efficacy was evaluated by direct application on skin during day time where mosquito bites are likely to happen. So aim of this study is to explore the different usages of some selected traditional Guli and Kalka available in Ayurveda hospital. Suranvidura Vati. Pengiri oil is time tested oil for such bites but cannot be applied to skin. mosquito repellant activity 187 . second and third hour after application. Keywords: Pengiri baby oil. Five traditional Guli and Kalka available in Out Patient department (OPD) were selected for the study. Nawarathna Kalka and Seetarama Vati for vomiting. According to the results. Buddharaja Kalka and Seetharama Vati can used to treat Jvara. Navaratna Kalka and Chandra Kalka have over 40% of Anupana for diarrhoea. Seetarama Vati and Nawarathna Kalka for diarrhoea.Chandra Kalkaand Buddharaja Kalka is there to replace it by simply changing the Anupana. Based on the above results it is very difficult to come to exact conclusion However. Anupana PP 061: Preliminary survey on mosquito repellent activity of “Pengiri baby oil” LM Karunagoda Department of Ayurveda. Data were collected based on the structured questionnaire filled by parents. Suranvidura Vati. Children between the age group 1-4years were selected from 04 Gramaniladari divisions of Galle District between June to September 2017. Total 106 individuals of male and female were evaluated for first three hours and the repellent activity as observed by their parents were or guardian were collected after repeated application for consecutive seven days. Sri Lanka Prevalence of mosquito bites and its consequences are very high during last few months in Sri Lanka and there were very few market preparations to prevent such bites.There are many Guli. In the absence of Seetharama Vati for Jvara. Among the Anupana for jvara 26% of Anupanas mentioned in Chandra Kalka and Buddharaja Kalka and 53% in Seetharama Vati. According to the results Chandra Kalka. The relevant data were collected from Unani authentic texts. The prevalence of heamorrhoids is estimated to be 4. exercise therapy. Improvement was evaluated by pain scale 1-10 for pain reduction and pre-and post-x-ray imaging for curvature of spine. University of Colombo. scientific journals and through electronic media. The Unani poly herbal formula (Ithrifale Muqil) was prescribed internally and it relieves the condition effectively. Ithrifale-muqil is a time-tested drug which is prescribed for Bawaseer in the Unani system of Medicine. It contained 188 . who reported to the clinic with the complaint of chronic back pain for 5 years. Sri Lanka Bawaseer (heamorrhoids) is a common anorectal disorder. Study has revealed that integrated treatment modalities were effective in treating degenerative scoliosis. It is a lateral curvature of the spine (with twisting). Objectives of treatment were reduction of pain. Thirukkovil. MNF Rizniya Institute of Indigenous Medicine.PP 063: Efficacy of integrated treatment for degenerative scoliosis with Unani medicine and physiotherapy . This study was designed to evaluate the efficacy of Ithrifale-muqil in Bawaseer. heat therapy. Further. Degenerative scoliosis. postural managements and external application were used continuously for 3 months. Heamorrhoids are defined as the symptomatic enlargement and distal displacement of the normal anal cushion. University of Colombo. spinal asymmetry. maximal restoration of spine to erect position. Sri Lanka 2 Institute of Indigenous Medicine. Sri Lanka Adult degenerative scoliosis is prevalent in 68% of the asymptomatic population over 60 years. JF Nairoza2 1 Rural Ayurvedic Hospital. Muscular imbalance also involves the progression of this spinal asymmetry. Unani remedies help to restore muscular power and relieve muscular imbalance. was diagnosed as degenerative scoliosis. A 50 year old female patient. The patient showed 50% improvement within a week and 80% improvement after 3 months with absence of scoliosis and better quality of life with combined treatment of Unani medicines and physiotherapy. Balghami or GhaleezKhoon (morbid matter) and the disturbance in the Mizaj (temperament) of liver and intestine. leads to increased asymmetric load to the spinal column resulting in the progression of degeneration and deformity. Composite intervention with oral Unani medicines. it is recommended to implement long term and large sample studies in the future.A case study MFM Fahim1. muscular imbalance PP 064: A review on the efficacy of Ithrifale-muqil (Unani poly herbal formula) for Bawaseer (heamorrhoids) MJA Fazmiya. Keywords: scoliosis. Unani physicians describe that Bawaseer results due to accumulation of Ghair Thabayee Ahlath in particular vessels. Tafarruk-e-Ihtisal. type of Tafarruk-e-Ihtisal in Unani system of medicine (siscontinuity of structure). Scoliosis is commonly ignored until gross cosmetic effects or signs of structural destruction were witnessed. Qabs (constipation) is an associating factor for Bawaseer. prevention of more curvature. caused by asymmetric degeneration of the facet joint and inter vertebral disc.4%. in the worldwide population. Results suggest that the main causes for the development of haemorrhoids are Sawdavi. with a view to explore better patient care management through Ayurveda. adults. Majority (83%) were experiencing the first episode of dengue infection and 17% were having a secondary episode. immune-modulatory. However. Demographic data and data related to dengue infection were obtained and Prakṛti statuses of the participants were assessed. Therefore it could be concluded that Ithrifale-muqil is effective in the management of Bawaseer. Mushil Bulgham Vo Sawda. Amila. Hence. anti-inflammatory and analgesic effects for the ingredients of Ithrifale Muqil. Vāta. determined at the time of conception. Baleela. an observational study to determine Prakṛti status of adults suffering dengue. The virus acts as an external factor and. In the study. Dengue is a viral infection affecting homeostasis of Tridoṣa. laxative. Every individual is born with a unique combination of Doṣas. W Weerapana. Among them 77% suffered dengue fever and 23% dengue heamorrhagic fever. DafeBawaseer actions. Mulayin (laxative). A number of thirty adults who suffered dengue infection were interviewed and. dengue 189 . it can be concluded that the individuals whose Pitta Doṣa is dominant were highly susceptible to dengue in this study. For this reason. male representation was 53% and female was 47%. Prakṛti means innate character and physical constitution of a person which. These ingredients have digestive corrective. Pitta- KaphaPrakṛti were 37% each and Kewala Pitta was 23%. seasonal variations. signs and symptoms of dengue are varying and the majority is asymptomatic. was performed. Further texts suggest these correct the liver and stomach and rectify ill temperament of the organ. wound healing. heamorrhoids (Bawaseer) PP 065: A study on Prakṛti status (body constitution) among adults suffering Dengue MRS Peiris. Assessing individual’s Prakṛti status showed that participants who belong to Pitta-Vāta and. Maweez and Honey. Prakṛti is also involved as a prime internal factor in dengue infection. Keywords: Itrifale. prepared questionnaires were filled by Ayurveda medical officers. Sri Lanka According to Ayurveda. lifestyle changes and several internal and external factors influence it. hepato-protective. A minority develops mild to severe signs and symptoms and it is difficult to predict who will develop into the critical phase.muqil. Although the person’s body constitution is not changed throughout the life time. mental and behavioral features. V Senevirathna Bandaranaike Memorial Ayurveda Research Institute. Munzij (coction). Keywords: Prakṛti. and Kapha. describes ones physical.Haleela. Scientific research describes pharmacological actions such as gastro-protective. Pitta. it is much similar to Gudrasi. Sinhāsya Pañcamūlī Kaṣāya. NSDM. Aśwagandhā Rasāyana. Keywords: sciatica. Kativasti with Nārāyana. Yogarāja Guggulu. SR Abeysooriya2 1 Department of Cikitsa. Rāsnā Aśwagandhā fermentation in third week. Sciatica may begin either suddenly with physical activity or slowly. AbaMurungā fermentation (FAM) in first week.PP 067: Combination of Ayurveda regimen and wet cupping in management of sciatic nerve compression . by using multiple Ayurvedic treatment modalities . paedic paste (PP). University of Kelaniya. The patient conceived within 2 months of treatment and she continued to follow further treatments up to the birth of a healthy female baby. Sri Lanka 2 Department of Dravyaguna.A case study LSB Nuwansiri1. but recent authors categorize this disease under the Vataki Yoni Vyapat. the patient was suffering from sciatic pain due to disc herniation of L4-L5. Gudrasi. Keywords: Endometriosis. University of Kelaniya. VāsāguducīKaṣāya . Endometriosis is not directly mentioned in Ayurveda texts. Gampaha Wickramarachchi Ayurveda Institute. an effort was made to treat a 35 year old female with clinically diagnosed grade Grade IV Endometriosis associated with subfertility. but the problem tends to return and women need to go for repeated surgeries. who presented to the Subfertility clinic of Gampaha Wickramarachchi Ayurveda Teaching Hospital. The symptomatic reduction was evaluated with Sciatic Bothersomeness Index (SBI) and Sciatica Frequency index (SFI) and conformed with Magnetic Resonance Imaging (MRI). According to Ayurveda signs and symptoms. Of the sub-fertile women. 20-50% are affected by this disease. The pain that extends from the buttocks down the course of the sciatic nerve is common. The patient was treated with wet cupping. Endometriosis is normally managed using surgical therapy in Allopathic treatment. Gampaha Wickramarachchi Ayurveda Institute. Sciatalone. Nearly 90% of cases are associated with a disc disorder. In this case study. University of Kelaniya. Sri Lanka Endometriosis is defined as the presence of functioning endometrial tissue outside the uterus. Gampaha Wickramarachchi Ayurveda Institute. wet cupping PP 068: A case study on Ayurvedic management of Grade IV Endometriosis associated with Sub- fertility HP Wakkumbura. The fourth and fifth lumbar nerve roots and the first three sacral roots joint in the lumbar sacral flexes to form the peroneal and tibial nerves and they leave the pelvis in an ensheathed single trunk as the sciatic Nerve. The patient recorded remarkable reduction in symptoms to continue daily routine activities after the treatments. respectively. Sciatic pain has aching and sharp components and radiates along lower limbs or lower buttock. SA Dissanayake Department of Kaumarabruthya and Stree Roga. Nādī Sweda with Daśamoola Kwātha (NSDM). Here. FAM in second week and Māṣabalā Kaṣāya. Endometriosis affects 10% of women of reproductive age. Sri Lanka The sciatic nerve is the largest nerve in the body. Vataki Yoni Vyapat 190 . Both internal and local treatments were administered to the patient including patyapatya (dietary pattern and behaviors). HP Wakkumbura Department of Kaumarabruthya and Stree Roga.15 mIU/ml for FSH level and LH level. Migraine headache attacks may be triggered by. PKH Dharmawijaya Gampaha Wickramarachchi Ayurveda Institute. odors or perfumes.9 mIU/ml of LH). an effort was made to treat a 35 year old female with clinically diagnosed secondary amenorrhea (with 114. After 6 months of treatment. Gampaha Wickramarachchi Ayurveda Institute. using Ayurvedic treatment modalities. bright lights. physical or emotional stress. In women.O has ranked migraine as number 19 among all diseases worldwide causing disability. Artava. People often turn to Ayurveda physicians for desperate conditions. MNM Jayarathne. intensity of pain and associated symptoms in 191 . Keywords: amenorrhoea.5 mIU/ml and 5. University of Kelaniya. which subsequently stimulates the corpus luteum to produce progesterone. changes in sleep patterns. Ayurveda is a nearly 3000 year old traditional medical system. including patyapatya (dietary pattern and behaviors). respectively. pathology. pills and tablets.H. symptoms and treatment principles. Here. secondary amenorrhea is described as destruction of Artava (Nastratava) as well as one of the symptoms of Artav-vaha strotas viddha lakshna. and the secretion of estrogen from this follicle is dependent on FSH and LH. University of Kelaniya. A surge of LH in the mid menstrual cycle is responsible for ovulation. All subjects completed the 90 days of Ayurveda treatments. Follicle-stimulating hormone (FSH) is a glycoprotein gonadotropin secreted by the anterior pituitary in response to gonadotropin- releasing hormone (GnRH) released by the hypothalamus. It can be correlated with migraine. Sri Lanka Ardhāvabhedaka is one among the eleven types of Śiroroga. the patient recovered from the amenorrhea condition with the laboratory investigations of 18. This treatment brought significant relief in reducing the frequency. According to Ayurveda. W. Both internal and local treatments such as taila abyanga were administered to the patient. including decoctions. The pituitary gland also secretes luteinizing hormone (LH). in the absence of pregnancy and lactation. another gonadotropin. Sri Lanka Secondary amenorrhea is defined as Amenorrhea of 6 months or more in a woman with previously normal menstrual patterns. skipping meals and alcohol. The purpose of the study was to assess the effect ofBalā Bilva Śuṇṭhi Kaṣāya available at market in the management of Ardhāvabhedaka. LH stimulates estrogen and progesterone production from the ovary. allergic reactions.20patients suffering from Ardhāwabhedaka were registered and givenBalā Bilva Śuṇṭhi Kwātha 30 ml thrice a day for oral administration for a duration of 3 months. She presented to the Gynecology clinic of Gampaha Wickramarachchi Ayurveda Teaching Hospital. Development of the ovarian follicle is largely under FSH control.PP 069: A case report on the Ayurvedic management of amenorrhea associated with high levels of FSH and LH Hormones SA Dissanayake. smoking or exposure to smoke. FSH.3 mIU/ml of FSH level and 18. loud noises. LH PP 070: A clinical study on the management of Ardhāvabhedaka RMD Jayasena. Migraine is the second most common cause of headache. and continued LH secretion. based on the similarity in etiology. Keywords: Gridhrasi. migraine. 51% of the patients improved moderately while mild improvement was observed in 47. It is a pain dominant disease and reduces human activity considerably in terms of personal as well as social and professional life. photophobia. visual disturbances and tenderness showed high statistical significance with p value <0. stinging or numbing pain that is felt in the buttock. Mīpotu thewilla. In this study patients who suffered from Gridhrasi since 1 month were treated with traditional Sri Lankan pottalī called Mīpotu thewilla. thigh. PKH Dharmawijaya Gampaha Wickramarachchi Ayurveda Institute. Pilathbeblila Kasaya 120ml 2bd. movement and stability were poor.001. A total of 30 patients of Gridhrasi were registered and fomented with Mīpotu thewilla twice a day for 14 days. A visual analogue scale for pain. Radiology (X-ray) report confirmed joint space narrowing with oesteophyte formation. Gridhrasi (sciatica) is one among Vāta-vyādhi caused by aggravated Vāta Doṣa. MNM Jayarathne1. Balā Bilva Śuṇṭhi Kwātha PP 071: Effect of traditional fomentation in the management of Gridrasi RMD Jayasena. Sri Lankan traditional and Ayurveda medicine treatment was given for 90 days with external operation (Thrayodashanga Gugul 2 bd. the Knee Society Rating System scores of pain.migraine patients. Pottalī showed better effect on patients of VātaKaphaja type of Gridhrasi in comparison of Pittaja type of Gridhrasi. sciatica PP 072: A case study on the management of SandigataVata RMD Jayasena1.09% of the patients related to the severity of pain. MNM Jayarathne. The result on severity of pain showed high statistical significance with p value <0. and the function score was fair. It may or may not be associated with low back pain. vomiting. The result on duration of pain showed highly significant effect with P value< 0. University of Kelaniya.001. leg or foot. knee scores in the Knee Society online rating system and an Ayurveda clinical assessment criteria was used to evaluate the effects of treatments on a weekly basis. the Knee Society Rating System scores of pain. It is characterized by burning. movement and stability were improved up to a 192 . The result on frequency of attack showed highly significant effect with P value <0. PKH Dharmawijaya2 1 University of Kelaniya. Eleven subjects reported significant improvement in overall symptoms of migraine. After the treatment. Vata Viduranga oil 60ml for external application). Sri Lanka Low back pain is one of the common conditions of loco motor system disorder and affects people during their productive life.100. The results on nausea. Keywords: Ardhāvabhedaka. About 40% of cases of low back pain are radicular in origin and considered under the category of sciatic syndrome. Sri Lanka A 56-year-old female with end-stage osteoarthritis (OA) (Sandhigata Vata) of the right knee joint is reviewed here. Sri Lanka 2 Gampaha Wickramarachchi Ayurveda Institute. After treatment for 90 days.100. Prior to treatment. vertigo. Overall assessment showed statistically highly significant results with the p<0. The purpose of the study was to assess the effect ofMīpotu thewilla on the management of Gridhrasi. 70% of patients developed SLRT test up to 900.100. ‘good’ level and function score was improved up to ‘excellent’ level. hemorrhoid PP 074: Evidence based effects of ingredients of Navaratna Kalka on pain modulation of primary dysmenorrhea KNA Dharmasena. Wild Celery. and mucous discharge. The objective of the study was to evaluate the antibacterial action and clinical effect of Panchawalkala in the management of Hemorrhoid. etc. coli and P. Clinical diagnosis was done by assessing the signs and symptoms of international criteria for hemorrhoids such as. Staphylococus aureous is highly active in both medium. ND Kodithuwakku Institute of Indigenous Medicine. Sri Lanka Navaratna Kalka (NK) is one of the valuable drugs of the traditional medical system. Sri Lanka The word Pañcawalkala is used frequently in Nighantu Granthas like Dhanvantri. It comprises 15 herbs. agalactea. Nevertheless.Raja. In conclusion. The antibacterial activity was classified as highly active (>20 mm). Licorice. E. bleeding from rectum. University of Colombo. Sri Lanka 2 Department of Shalya. Pseudomonasaeuruginosa is mildly active in medium amoxicillin and inactive in Pañcawalkala medium.e. The antibacterial activities of Pañcawalkala Avagaha were tested against S. Aththikka (Ficus racemosa). aureus. Clinically Pañcawalkala i. Pulila (Ficus lacor) and Wewel (Calamus rotang). this OA patient's quality of life was improved by the combined treatment of Sri Lankan traditional medicine and Ayurveda. Pañcawalkala is one of the ideal combinations for a vast range of therapeutics focused in Ayurveda. Keywords: Pañcawalkala. Keywords: Sandigata Vata. Bhavaprakash etc. it is not recommended 193 . E-coli is highly active in medium amoxicillin and inactive in Pañcawalkala medium. Shothahara action. joint symptoms and signs and the knee scores were unchanged. Bo (Ficus religiosa). prolapse. slightly active (12-15 mm) and less than 12 mm was taken as inactive. The present study revealed Streptococus is highly active in medium amoxicillin and mildly active in Pañcawalkala medium. University of Kelaniya. used in ‘pain’ prominent diseases. AAJ Pushpakumara2 1 Institute of Indigenous Medicine. mild active (15-20 mm). osteoarthritis PP 073: Antibacterial action and clinical effect of the Pañcawalkala in the management of hemorrhoids MIJ Jayakody1. In this study 60 patients with hemorrhoids (grade two) were randomly selected and patients were reviewed on a weekly basis for a total of eight weeks. Clove. Gampaha Wickramarachchi Ayurveda Institute. groups of barks of five trees Nuga (Ficus bengalensis). NK is found to be effective in Kaṣṭa Rajas (which can be correlated to dysmenorrhea) in general practice. Zone of inhibition of extract were compared with medium of amoxicillin. mainly Terminalia chebula and Terminalia bellirica with 4 varieties of Fennels. aeuruginosa human pathogenic bacteria. pain. itching sensation. S. During the follow-up period. University of Colombo. is found to be very effective in Vrana shodana and also has Grahi. antibacterial. Margosa has also shown toxicity against fungi including Trichophyton. swelling. Literature was searched to assess potential antibacterial and antifungal properties of Margosa. Relevant traditional texts. nimbin. Moreover. NK should be considered as a potential drug for dysmenorrhea. convincing RCT evidence supported the efficacy of individual herbs of NK for dysmenorrhea. KaṭuRasa. Rajarata University of Sri Lanka Margosa (Azadirachta indica) is a well-known medicinal plant. whereas Kaṣṭa Rajas as a feature of vitiated Apāna Vāta due to misuse of food and behavior. It was reported that Margosa contains active substances with multiple medicinal properties. azadirachtin 194 . Further. Uṣṇa Vīrya and Madhura Vipāka as predominant properties synergistically. Based on these. Further. 31 Randomized Controlled Trials (RCT) and recent research studies of ingredients on analgesic effects were selected. used widely in folk medicine in Sri Lanka and India. DNM Gunathilaka. this study was designed to analyze evidence based efficacy of NK in pain modulation of ‘Kaṣṭa Rajas’. However. Pseudomonas aeruginosa. gallic acid. Epidermophyton. due to their adverse effects. Analytical study of Ayurveda pharmacological properties of NK showed. clearing gingivitis and reducing plaque. bark. Staphylococcus aureus and Enterococcus faecalis. Ayurveda analytical studies validated the capability of NK for pacifying vitiated Apāna Vāta in Kaṣṭa Rajas. Proteus mirabilis. Trichosporon. Conventional treatments for dysmenorrhea include NSAIDs. Cochran and Science Direct were searched to acuminate scientific evidence. treating acne. pain modulation PP 075: Potential of Azadirachta indica (Margosa) for controlling bacterial and fungal pathogens- A review WMNDK Wijekoon. nimbolide. even in the modern era. Geotrichum and Candida. liver and kidney damage with the excessive doses. Yet these do not provide adequate relief of pain and symptoms. Microsporum. Laghu and Rūkṣa Guṇa. It can be concluded that Azadirachta indica can be used as a potent biocide to treat various bacterial and fungal infections in both traditional and complementary medicine. antifungal. The other antibacterial agents are nimbidin. Hence. Keywords: Azadirachta indica. Scopus. contraceptives and COX inhibitors in an attempt to help the pain causing factors such as. Faculty of Applied Sciences. Ayurveda texts and electronic databases such as PubMed. safe and effective treatments are still warranted. Thus. Its leaves. researchers have shown that leaf extract of Margosa can be used against human pathogens such as pathogenic strains of Escherichia coli. Klebsiella pneumoniae. PN Yapa Department of Biological Sciences. some components of Margosa can cause side effects such as rashes. hyper- contractility. It was evidenced that Margosa oil has suppressed Salmonella typhosa. epicatechin. Kaṣṭa Rajas. In conclusion. dysmenorrhoea. Identified analgesic effects of ingredients were similar to NSAIDS and COX inhibitors. antibacterial. The main active constituent of Margosa is azadirachtin which is an effective antimicrobial agent. catechin and margolone. prostaglandin E concentrations. all the ingredients of NK have evidence based analgesic effect on dysmenorrhea. traditional medicine. Keywords: Navaratna Kalka. AGD Pavithra. Margosa leaves have antibacterial activity which could be used for controlling airborne bacterial infections. though these occurrences are rare. fruits. Furthermore. seeds and oil which contains antibacterial and antifungal compounds can be used to treat various health conditions. These properties play a major role in treating vaginal infections (including chronic Candida infections and bacterial vaginosis).in Kaṣṭa Rajas authentically. A review KMSM Gangathilaka. Stones may form because the urine becomes too saturated with salts and when urine lacks the normal inhibitors (eg.PP 076: Kalanchoe pinnata (Akkapana) as a herbal treatment for urinary stones . The disease results in a characteristic skin rash that forms itchy blisters. Kalanchoe pinnata. RKR Wasana1 1 Ayurveda Central Dispensary. Healthy skin reflects a healthy body condition. DMM Bandaranayake Department of Biological Sciences. curry and salad made from tender young shoots and young leaves for a period of 10 days. bleeding. after recovering from fever. Urinary stones have gained increasing significance due to changes in living conditions. folds and lines with aging and scars and scabs due to various diseases. With the untreated patient. Two patients. triterpenes. citrate) of stone formation. Stones are hard masses that form in the urinary tract and may cause pain. cultural acceptability and lesser side effects as compared to synthetic drugs. scabs 195 . Hence this study was carried out. Costus speciosus (Sinhala: Thebu) is a succulent perennial herb with spirally arranged leaves. alkaloids. All the scabs disappeared after 12 days in the patient who was given CS. or struvite. Skin gets wrinkles. which eventually scab over. One patient was given CS prepared as Mellum. Keywords: crystals. such as lack of drinking water. It can also enhance the reducing properties of the calcium oxalate crystals by reducing its size and it promotes the formation of calcium oxalate dehydrate crystals rather than monohydrate crystals. calcium oxalate PP 077: Effect of Costus speciosus in eradication of Chickenpox scabs – A case study VGSI Nuwan1. So far no scientific study has been conducted to observe the effect of CS on chickenpox scabs. PWIM Chandrasekara. aged between 20 to 30 years. Keywords: chickenpox. It helps in the treatment of urinary stones by stimulating the production of urine. cystine. Consuming Costus speciosus (CS) in the form of Mallum. efficacy. Moreover the size of the crystals gradually reduces with the increasing concentration of the extracts. Chickenpox is one such disease with many effects to the skin. Stones are made of minerals in the urine that form crystals. urolithaisis. including uric acid. ERHSS Ediriweera2. This study supports the idea that consuming CS helps to reduce scabs arising from chickenpox faster. infection or block the flow of urine. Many people suffer from common diseases like kidney stones. having scabs from chickenpox were selected. Faculty of Applied Sciences Rajarata University of Sri Lanka Herbal plants have been used throughout human history for various purposes such as therapeutic agents for the prevention of various ailments. University of Colombo. Both patients were not subjected to any other medicine. It is an important medicinal and ornamental plant cultivated in moist and evergreen areas. Thus it can be used to control urolithiasis. Sri Lanka Skin is the largest organ of the human body. Costus specious. Herbal plants have been used for centuries for this problem due to its safety. Boliyadda. a commonly used herbal plant contains important chemical constitutes like malic acid. glycosides and flavonoids. gall stones and urinary stones. curry and salad in treatment of scabs resulting from chickenpox is a home remedy practiced in Sri Lanka. About 85% of the stones are composed of calcium and the remainder are composed of various substances. JAAWL Jayasekara. scabs disappeared after 32 days. The other patient was kept on a normal diet. Sri Lanka 2 Institute of Indigenous Medicine. 1) were the main acupuncture points used. Shenmen (H. Taiyuan(Lu. Sri Lanka 2 Sir Anton Jayasuriya International College of Acupuncture. the efficacy of a herbal combination (A3) consisting of Ipomoea mauritiana. The patient was under medication for diabetes mellitus (DM) for 5 years and felt numbness in both soles. with written consent. Asparagus gonoclados andWithania somnifera in the management of oligospermia was evaluated using a randomized controlled trial. According to World Health Organization. it is a main stream of Traditional Chinese Medicine (TCM). Baihui (Du. pulse rate 74/min.1). HP Wakkumbura2 1 Department of Ayurveda.9). The treatment was initiated with principles of acupuncture. The present case was retrospectively studied at Sir Anton Jayasuriya International College of Acupuncture in 2016. but failure to achieve a clinical pregnancy after 12 months of trying. Kurunegala. acupuncture PP 079: A clinical study of selected herbal powder (Ipomoea mauritiana. Investigations revealed she was R/CTS positive. up to 23% of men investigated have infertility. The physical examination revealed. University of Kelaniya. The patient was asked to continue physiotherapy along with Acupuncture management. Data were collected using 196 .20). a painful condition of the hand & fingers due to the compression of the median nerve where it passes over the carpal bones at the wrist. Lifestyle modification and proper day to day activities are essential in addition to treatment which prevents nerve and muscle damage in fingers and hand. SS Hettige2 1 Bandaranaike Memorial Ayurvedic Research Institute. Sri Lanka In acupuncture.4). initial blood pressure 130/70 mmHg. BMI 25kgm-2. It was noticed that the patient felt absence of pain. 44).I. Asparagus gonoclados and Withania somnifera) in the management of oligospermia (Kṣīṇaśukra) AMT Samantha Sri1. oligospermia (Kṣīṇaśukra). numbness and improvement in gripping ability. In this research. 34). a low sperm count is one of the main causes of male infertility. Sri Lanka Infertility (vandhyatva) is a condition of couples that have regular intercourse without using any contraception. Hence this case supports the idea that Acupuncture treatment (along with physiotherapy) is effective on CTS.PP 078: Efficacy of Acupuncture on Carpal Tunnel Syndrome – A case study KGCP Gamage1. Of the causes of infertility. Sri Lanka 2 Department of Kaumarabhuthya and Stree Roga. Yakkala. Two groups (test and control) of 30 patients were randomly selected from volunteers of low sperm counts patients at the infertility clinic of Gampaha Wickramarachchi Ayurveda Teaching Hospital. Test group was treated with A3 powder and control group with Triphalā cūrna for 3 months.B. Gampaha Wickramarachchi Ayurveda Institiute. Yingtang(Ex.7). Yanglingquan (G. Keywords: Carpal Tunnel Syndrome. Hegu (L.7). Lieque (Lu.6). Yungchuan (K. fine needles are inserted in the skin at specific points along the paths of energy which are considered as the windows of vital energy in our body. The patient was a 60 years old female who presented with the symptoms of numbness of the right hand and thumb and difficulty in gripping objects. This study aims to assess the efficacy of acupuncture treatment in Carpel Tunnel Syndrome (CTS). As a safe and effective holistic medicine. Neiting ( St. Neiguan (P. Leech therapy in Pitta predominance. Kṣīṇaśukra. Blood-letting is one of the most important and widely practiced methods of cleansing therapy used for local evacuation of morbid humours. an attempt is made to discuss the scientific basis of traditional bloodletting therapy. Leech therapy may be a better alternative to manage Pitta diseases. thrombolytic. antioxidant.A review U Saranya Unit of siddha medicine. Pitta and Kapha. and incision in the case of Kapha predominance. analgesic. excessive heaviness and musculature etc.1).05 was considered significant. Eastern University of Sri Lanka The traditional medical system is based on the concept of equilibrium and balance of natural body tri humours known as Vāta. Keywords: cupping therapy. Results found that cupping therapy is supposed to treat Vāta disease in traditional medicine by stimulating nerve points or meridians. cupping and incision. drain abscesses and remove tumors. The imbalance in the quality and quantity of these humours leads to diseases. hemodynamic. Gathered traditional knowledge and scientific data from current research were compared and analyzed critically. Three types of bloodletting procedure has been practiced from ancient times . In this review article. analgesic. Those actions have Vāta pacification properties such as counter irritation. and anti-coagulant and blood circulation enhancing properties. Therefore this study found that A3 powder had the ability to increase the sperm count and volume so can have a significant effect in the management of oligospermia. tri humours 197 . Trincomalee Campus. A number of scientific studies reveals the mechanism of cupping therapy. vasodilator. Pitta and Kapha disorders. As per Siddhas statement.leech therapy. Keywords: infertility. incision. Searches were performed in August 2017 through traditional text books and web sources for current research. immunomodulation. anti- asthmatics etc.Seminal fluid analysis before and after treatment. oligospermia. There are no studies describing why Siddhas mentioned cupping. and in practice incision is made to evacuate local blood congestion. leech and incision in order to treat Vāta. Results showed that there is a significant increase of the concentration and volume of sperm in test group patients (135% and 47%) compared to the control group (7% and 22%). P<0. leech therapy. Leech saliva contains numerous bioactive constituent which possesses anti-inflammatory. Data were statistically analyzed using student’s t – tests on SAS (9. Incision is used to treat Kapha aggravated conditions such as congestion. Its mode of action depends on the injection of leech saliva into patient's tissues during the process of blood sucking. cupping therapy is an important measure in the case of Vāta predominance. vandhyatva PP 080: Traditional bloodletting therapy and scientific truth behind it . called Indralupta in Ayurveda. Four treatment regimens were applied. exudates. Finally the wound was dressed using gauzes and supportive bandage. He had failed multiple therapies owing to persistent pruritus of the wound.9%. difference of the surface area of the scalp in all patients were increased significantly (P<0. Considerable changes were observed after three weeks of treatment as the wound was dried from moderate serous exudates. After two months of the treatment. a medium sized leech was selected and purified using turmeric water. Following purification process the leech was applied on the edge of the wound and kept until it released spontaneously. The heavy foul smell disappeared and granulation was promoted with the edges starting 198 . Sri Lanka Chronic wounds present a challenge that is costly in terms of quality of life to the patient.A case series RP Mendis. During the treatment. In Deśīya Cikitsā it is called Udugovvan Kema. roots of Niyangala with lime juice was applied over the hair losing area and scalp was washed with the decoction of Hīn Nidikumba. Śamana and Tarpaṇa. Trincomalee campus. For this study 5 patients were recruited and surface area of the hair growth was assessed within two weeks.05). Ayurveda treatment protocol PP 082: Effect of leech therapy in a chronic infected wound – A case study U Saranya. Difference of the treatment effect was calculated by using Wilcoxon Signed Rank test with statistical software of SAS 9.1. Jaffna. smell. AMHS Attanayake Bandaranaike Memorial Ayurveda Research Institute. appearance of wound base and wound edge. EUSL. Sri Lanka 2 Rural Ayurvedhic Hospital. Concepts of the treatments were Śodhana. After leach therapy Poongavi Chendooram was applied on the leech bite to control bleeding and kept for around 15 minutes. This is a case report. partial loss of hair of the scalp was noted. A follow -up large sample study will be important to assess the efficacy of this Ayurveda treatment protocol. Before applying the application scalp was rubbed with the rough side of the Sepālika leaf. In the first four weeks Visarpahara oil was applied with flesh and seeds of Batu with lime juice to the scalp. In the last four weeks. Leech therapy is one of the ancient and important para-surgical procedures described in siddha medicine for treatment of various diseases including chronic wounds. Alopacia areata. The patient is a 69 year old male patient who suffered from two non-heeling ulcers in the lower part of the right leg for 3 years. The patient’s wound was initially cleaned using Normal saline 0. Sri Lanka Indralupta is the loss of previously existing scalp hair and is termed Alopecia areata. The patient was asked to obtain a full blood count and bleeding and clotting time to confirm normal hemoglobin and exclude bleeding disorder for leech therapy. The aim of this study was to identify the hair growing activity for Ayurveda treatment protocol for Indralupta. These results suggest that using Ayurveda treatment and knowledge can improve Allopacia acreata. Karavaddi.PP 081: Assessing the therapeutic effect of Ayurveda treatment protocol for Indralupta (Alopecia areata) . Effectiveness of traditional leech therapy was evaluated based on improvement in the wound size. M Kanesalingam 1 Unit of Siddha Medicine. Same treatment procedure was carried out every week until the wound completely cured. Keywords: Indralupta. University of Colombo. The majority of patients were female (95%) and in 46 – 50 age group (52. 2 groups were examined. of Deshiyachikitsa.2×3. and group B (20 patients) was treated with Rasonadi Decoction (RD)for a period of one month and the follow up period was the same. Gauravaandpipasa was improved by ED in a significant manner (p<0.05). pulse rate. complications and its morbidity.7 and 8. WBC and lymphocyte count in a highly significant manner (p<0. A fever may also accompany the abscess. Group A (20 patients) was treated with Erandadi Decoction (ED). Sri Lanka Bartholin's abscess is an acute infection of a Bartholin's gland with symptoms including pain.5%). RD improved fever right and left sided knee joint swelling. Rheumatoid Arthritis. Sthabdhata. Gampaha Wickramarachchi Ayurveda Institute.001) whereas Sthabdhata. The wound was completely cured after 12 weeks of treatment as the wound size was reduced from 7. pacifying and regenerative procedure. WBC and lymphocyte count in highly significant manner (p<0. it was evident that RD is more effective over ED in improving subjective as well as objective parameters of Amavata. Western Province. Erandadi Decoction PP 084: Efficacy of a specially prepared herbal cream in the management of Bartholin's abscess WLARS Pushpakumari. fever.6 to 0cm. Gaurava and Pipasa wasimproved by RD.5%) while it was relieved by rest (75%). Clinical features were that symptoms were aggravated by cold water (75%). This case suggests that Leech therapy is an effective way to treat chronic infected wounds by its cleansing. pulse rate. Sparsa-ashaktataandnidravi-pryaya in a highly significant manner (p<0.to contract. In this study. Data were analyzed by SSPS statistical software. Aruchi. Keywords: Amavata. the majority of patients had Vata-Kaphaprakriti (60%). located on either side of the opening of the vagina. poor prognosis.05). Clinical appearance of this condition is severe pain with walking and sitting. swelling.001). Sri Lanka 2 Dept. Considering prakriti.001) whereas Nidra-Vipryaya. becomes infected.001). Sparsa-Ashaktata and Aruchi in a highly significant manner (p<0. RD improved Sandishoola. SMS Samarakoon2 1 Department of Ayurveda. leech therapy. GUA Kumara Department of Kaumarabhritya and Striroga. Siddha medicine PP 083: A comparative study on the efficacy of Erandadi and Rasonadi decoctions on Amavata (Rheumatoid Arthritis) TD Dayawaradana1. The aim of this study was to evaluate the efficacy of a selected herbal creamin the 199 . Rasonadi Decoction.3cm×4. When comparing the effects of ED and RD. Sri Lanka Amavata is one of the challenging diseases in Ayurveda due to its chronic nature. University of Kelaniya. cellulitis of the vulva and dyspareunia. Erandadi Decoction (ED) improved Sandishoola. Some herbal creams contain herbal drugs which have Shodhana and Shamana properties. ED improved ESR. Institute of Indigenous Medicine. walking (60%) and rainy weather (57. which is significant (p<0. A Bartholin’s abscess can occur when one of the Bartholin’s glands. Key words: chronic wounds. right and left sided knee joint swelling. fruits of chebulic myrobalan (Terminalia chebula Linn). Data was collected before. In modern medicine it is correlated with conditions of obesity and overweight. The present study was carried out to evaluate the efficacy of Tseraobe Tea in the management of Sthaulya (obesity and overweight). WLARS Pushpakumari Gampaha Wickramarachchi Ayurveda Institute. Tseraobe Tea is a herbal preparation which contains Lekhaneeyadravyas mentioned in Ayurveda classics. BMI was calculated before. After collecting a detailed patient history and physical examination they were advised to take 2 tea bags per day at 9. Bartholin’s gland.5) suggested that Tseraobe Tea is an effective and safe herbal formulation in reducingSthaulya (obesity and overweight) in adults. during and after two months of a follow up period. Keywords: Sthaulya. Sri Lanka Abnormal or excessive fat accumulation of the body is known as Sthaulya. cellulitis of the vulva. University of Kelaniya. No adverse effects were reported. Sri Lanka Artavakshaya is defined as an irregular menstrual cycle. University of Kelaniya. The analysis (indicated p-value is p<00. Hence. BMI. herbal cream. The Pippalimoolamabhaya Peyawa which includes roots of black pepper (Piper nigrum Linn).00 a. swelling. Dysmenorrhea is divided into two categories: primary (occurring in the absence of pelvic pathology) and secondary (resulting from identifiable organic diseases). More than 35% of people suffer from obesity and overweight in the present world. Further. Lekhaneeyadravya PP 086: Artavakshaya (primary dysmenorrhea) . Gampaha Wickramarachchi Ayurveda Institute. deficiency of amount of menstrual blood or pain in the vagina (painful menstruation).m. immediately after and after 14 days of a follow up period.management of Bartholin's abscess. during. Results were analyzed statistically. swelling. continuously for a period of 2 months. Collected data were statistically analyzed.m.A case series GUA Kumara Department of Kaumarabhritya and Striroga. Yakkala. cellulitis of the vulva and dyspareunia PP 085: Clinical efficacy of Tseraobe tea in the management of Sthaulya .05). Body Mass Index (BMI) is the most commonly used method to classify overweight and obesity in adults.00 p. Sri Lanka. all the patients were advised to apply the herbal cream on the abscess twice a day for a period of seven days continuously. pain. The clinical trial was conducted on 20 patients diagnosed with obesity and overweight and aged over 20 years. After examination. and 3. It is correlated with dysmenorrhea in modern medicine. it can be concluded that selected herbal cream has an efficacy in the management of Bartholin's abscess. obesity.A case series GUA Kumara. Forty volunteer patients who were diagnosed with Bartholin's abscess were selected for the study in a Gynecological Clinic of Gampaha Wickramarachchi Ayurveda (Teaching) Hospital. The aim and action of the drug was explained to all patients. as well as dyspareunia. Keywords: Bartholin's abscess. Reduction of symptoms of Bartholin's abscess such as pain. patients were advised to follow the given Pathyapathya schedule. were statistically significant (p<0. roots of beal (Aegle marmelos Linn) and fried seeds of rice (Oryza sativa Linn) are 200 . 5% had marked improvement. no known scientific study has been conducted to evaluate the effect of Erabadu Pothu Melluma in Sandhigata Vata. and Prasarana Akumcana Janya Vedana (pain in flexion and extension of the joints). osteoarthritis. Sandhi Shotha (joint swelling or oedema). it is also known as Jara Vata(degenerative arthritis). 15% had moderate improvement. After obtaining written consent. In Ayurveda. For this study. symptoms of Sandhigata Vataare are described as Hanti Sandhigati (restricted movements). The reduction of clinical features was also assessed after two weeks of a follow up period. According to data analysis. Therefore. Keywords: Artavakshaya. The reduction of clinical features were also assessed after three months of a follow up period. It is mainly associated with aging. dysmenorrhea. On the basis of symptomatology it resembles osteoarthritis (OA) in modern medical science. Results were analyzed statistically. Sandhi Shoola. So far. during and after completion of the treatment using a specially prepared grading scale.05). degenerative arthritis. Yakkala in treatment of Sandhigata Vata with successful results. physical occupational activities and obesity. Severity of signs and symptoms were assessed before. 40 patients diagnosed with primary dysmenorrhea were randomly selected from the Gynaecology Clinic of Gampaha Wickramarachchi Ayurveda (Teaching) Hospital. The prepared Mellum was recommended to apply at night for a period of fourteen days continuously. deficiency of amount of menstrual blood and pain in the vagina (painful menstruation) were assessed before. Sandhi Shotha and Prasarana Akumcana Janya Vedana were statistically significant (p <0. University of Kelaniya. It is concluded that Erabadu Pothu Melluma is effective in the management of Sandhigata Vata. during and after completion of the treatment through a self-prepared grading scale. Hence. Irregularity of menstrual cycle. The clinical trial was conducted on 60 patients belonging to the age group of 35 to 60 years old.5% had complete remission. Keywords: Sandhigata Vata. Sri Lanka Sandhigata Vata is a disease mentioned in Ayurveda classics under the Vatavyadhi Prakarana. The purpose of this study was to study the efficacy of Pippalimoolamabhaya Peyawa in the management of Artavakshaya (primary dysmenorrhea). Erabadu Pothu Melluma is a traditional preparation practiced at Gampaha Wickramarachchi Ayurveda Teaching Hospital. Reduction of symptoms such as Hanti Sandhigati. it is concluded that Pippalimoolamabhaya Peyawa is effective in the management of Artavakshaya (primary dysmenorrhea). 60ml of Pippalimoolamabhaya Peyawa was recommended to be taken twice a day before meals for a period of seven days continuously. 27. It mainly affects weight bearing joints of the body especially hip and knee joints. Sandhi Shoola (joint pain). Pippalimoolamabhaya Peyawa PP 087: Evaluation of the efficacy of Erabadu Pothu Melluma in the management of Sandhigata Vata (Osteoarthritis) – A case series GUA Kumara Gampaha Wickramarachchi Ayurveda Institute. Erabadu Pothu Melluma 201 .recommended in the management of Artavakshaya.hence the present study was carried out. 37. 15% had mild improvement and 5% were unchanged. Vatavyadhi. The effect was assessed using a self- prepared grading scale according to the severity of the clinical features mentioned in above before treatment and after treatment. haematuria (83%). after food for a period of 45 days.00 am and 7. The period of the trial was one month. The main aim of this particular study was inclined towards the disintegration. Gampaha Wickramarachchi Ayurveda Institute. Group-II was treated with Flush out therapy (hydro-therapy). University of Kelaniya. Keywords: urolithiasis. Passiflora foetida 202 . diuretic and anti-lithic properties. Data was analyzed using Wilcoxon signed rank test for paired sample using Minitab statistical software. Group-I was treated with the decoction of Passiflora foetida at a dose of 45 ml. burning in the stomach.00 pm one hour before the meal. The selected syrup contains Aloe vera.05. size of calculi (74%) and number of calculi (68. Asperaragus gonoclados and sugar. dislodgement and expulsion of renal stones. This study was carried out to evaluate the efficacy of Gastri nil syrup in the management of Gastric hyperacidity. Glycyrrhiza glabra. twice daily. dysuria (76%). This study result shows that Gastri nil syrup has a significant effect in the management of gastric hyperacidity. Each group contained 15 patients. indigestion PP 089: Clinical evaluation of Passiflora foetida decoction in the management of Urolithiasis GVP Samaranayake. indigestion and constipation are the common signs and symptoms of Gastric hyperacidity. Burning in the stomach. P values for each feature were less than 0. Sri Lanka In the present study an effort was made to evaluate the efficacy of a decoction of the whole plant Passiflora foetida. It has very similar signs and symptoms to Amlapitta in Ayurveda. dissolution. The efficacy of decoction of Passiflora foetida in the relief of pain (76%). restlessness. nausea. These drugs are easily available. Keywords: gastric hyperacidity. AAJ Pushpakumara Department of Shalya Shalakya.7%) was highly significant. University of Kelaniya. Twenty patients who were clinically diagnosed with Gastric hyperacidity were randomly selected to the study under inclusion and exclusion criteria.PP 088: Evaluation of the efficacy of Gastri nil syrup in the management of gastric hyperacidity KGP Wijesekara. A total of 30 patients were selected randomly and were divided in to two groups. and also have anti-inflammatory. Hence this study suggests that traditional Ayurvedic management is effective and did not have any obvious adverse effects on the patients with Urolithiasis. GUA Kumara Gampaha Wickramarachchi Ayurveda Institute. Sri Lanka Gastric hyperacidity is a very common and troublesome disease in the present era. sour taste. economical and easy to administer. Zingiber officinale. Patients were advised to take 15ml of syrup at 7. burning in the throat. belching. MPN Pramodani.PP 090: Management of third degree heamorrhoids by Kshara karma. Keywords: Polyherbal Ayurvedic Formulation. WJ Wickramarachchi Gampaha Wickramarachchi Ayurveda Institute.A case report GVP Samaranayake. polyherbal ayurvedic formula was found safe and fairly well accepted by the patient. Kshara karma intervention was done. Kshara karma PP 091: Effect of a polyherbal ayurvedic formulation in Acne Vulgaris. with complaints of permanent prolapsing pile mass and bleeding while passing stool. University of Kelaniya. and carries significant emotional and psychosocial implications. University of Kelaniya. AAJ Pushpakumara Department of Shalya Shalakya. The etiology of hyperhidrosis is unknown and is associated with severe 203 . Sri Lanka Acne Vulgaris is the most common disease among the young generation.A preliminary study GVP Samaranayake. The case was diagnosed as a 3rd Degree Heamorrhoid with a bleeding tendency. Further. The pile mass and per rectal bleeding relieved in 8 days and the patient was relieved from all symptoms within 21 days. Keywords: 3rd degree heamorrhoid. The severity of acne and efficacy of treatment was assessed by Cook’s acne grading scale.01) as compared to pre-treatment scores. University of Kelaniya Sri Lanka Primary hyperhidrosis. The patient was followed up regularly from 2016 to the current period (2017) and proctoscopic examination did not reveal any evidence of recurrence of the Heamorrhoids. The results showed significant reduction in the Cook’s acne grading scores of the post-treatment (p<0. Polyherbal ayurveic formulation was then administered locally once at night for a period of 45 days. A preliminary trail was conducted in the Shalya Clinic in Gampaha Wickramarachchi Ayurvedic Hospital to assess the safety and efficacy of a polyherbal ayurvedic formulation in the management of Acne Vulgaris on scientific parameters. has a prevalence range of approximately 2% to 4% worldwide. It was therefore concluded that polyherbal ayurvedic formulation can be used safely and effectively for the treatment of Acne Vulgaris. Sri Lanka A 52 year old male patient came to the Anorectal Clinic of the outpatient Department of Gampaha Wickramarachchi Ayurveda Teaching Hospital. Yakkala. Twenty five patients were clinically assessed and diagnosed on the basis of a thorough history. No complications were reported after the procedure. a condition of sweating in excess of thermoregulatory requirements. Acne Vulgaris PP 093: A clinical study to evaluate the efficacy of Mudgadi Kalka in the management of Atisweda (hyperhidrosis) NWGND Gamlath. AAJ Pushpakumara Department of Shalya Shalakya. Gampaha Wickramarachchi Ayurveda Institute. Gampaha Wickramarachchi Ayurveda Institute. It can be concluded that the tested Mudgadi kalka was successful in treating Primary Hyperhidrosis in the sample group.6 and 73. after treatments = 142±3. According to the National Diabetes Fact Sheet 23. The Mudgadi kalka was prepared according to the guidelines of the traditional formula.emotional.05) reduction was noted in the fasting blood glucose level.5g of Mudgadi kalka twice daily for a period of one month and assessed for sweating by the visual scale and Hyperhidrosis Disease Severity Scale (HDSS). Body odour as a symptom also was reduced but statistically insignificant. The primary objective of this study was to determine the effect and efficacy of Trikuluadi kalka and to evaluate the clinical improvement of selected subjects with the oral administration of Trikuluadi kalka. Patients from both sexes were selected. Vigna radiata. FBS.5±13.53% (before treatments = 222±13.2±2. Sri Lanka Hyperglycemia or high blood sugar is a condition in which an excessive amount of glucose circulates in the blood plasma. This study suggests that Trikuluadi kalka had positive effects on Hyperglycemia in the sample group. 31.6 million children and adults (7.1.7 before and 79. The results revealed that 60% of patients showed significantly reduced levels of excessive sweating and burning sensation of the body than usual with P value of P<0. The test drug was prepared according to the guidelines in the traditional formula. The Diastolic Blood Pressure was 87. University of Kelaniya. It contains Sesamum indicum.5±8. occupational. and social stress. WJ Wickramarachchi Gampaha Wickramarachchi Ayurveda Institute.05.4 p<0. for the determination of mechanisms of actions and replication of the study in involving a larger sample during a lengthier period of time is suggested as of prime importance for the determination of effects and efficacy of Trikuluadi kalka.0 before and after treatments respectively during this period with dietary management. Keywords: primary hyperhidrosis. Chemical analysis of the raw materials. Thirty patients with confirmed hyperglycemic conditions were selected and all the patients were between 25-60 years of age.3 after and the Pulse Rate was 76. Trikuluadikalka 204 . Keywords: hyperglycemia. Thirty patients were evaluated in the present study to evaluate the efficacy of Mudgadi kalka prescribed in Traditional medicine in the management of Atisweda (hyperhidrosis). Zingiber officinale and other traditional drugs.7±2. Thirty patients were treated with the 7. Bbody odour and burning sensation of the body by grading system.5% of the population) in the United States suffer from this condition. Mudgadi kalka PP 094: A clinical study on the efficacy of Trikuluadi Kalka in the management of hyperglycaemia NWGND Gamlath. Fasting blood sugar. MPN Pramodani. pulse rate and blood pressure were assessed every two weeks before starting and after completing the course of treatments. 20 individuals with Anidra (with mild psychological stress) were selected. P Vitharana3 1 Gampaha Wickramarachchi Ayurveda Institute. SS Hettige2. Sri Lanka 2 Manasa Ayurveda Hospital. The present study was designed to determine the comparative effectiveness of Himajaladhara and Kumaripadma Snehadhara in the management of stress. The study 205 . To pacify the provoked Vatadosha. referring to Ayurveda Manobhava concepts. Gampaha Wickramarachchi Ayurveda Institute. Sri Lanka. Prolonged decrease of Satvaguna in the manas leads to the mind becoming stressed. Neelammahara. with approximately 45 minutes in every sitting. Neelammahara Psychiatry Traditional Neelakadi oil (NS) and Sathwawadi oil (SS) are two varieties of Sleep enhancers use in Dharas. In CA all the symptoms were assessed and the severity of each symptom was rated. along with a specific ‘sleep diary” assessment. as the procedure mentioned in Group I. Group II was administered SS. 2 Manasa Ayurveda Hospital. Snehadhara. Statistical analysis was done in the pre-treatment observation and post treatment recovery. This leads to psychological stress. Charaka Samhita mentions sleep is an important outcome of a relaxed mind. The aforesaid observations were made on 7 main symptoms. Sri Lanka According to Ayurveda. Constant use of sleep enhancers internally result in addictive and neuroendocrine side effects. Boralesgamuwa. The assessment was done with both subjective (SA) and clinical improvement (CA) measures. 90 individuals with Psychological stress were selected and grouped in to 3. Gampaha Wickramarachchi Ayurveda Institute. as 30 cases in each group. Group I was administered NS for 15 consecutive days. Parinama (time factor). SS was found to have the most significant effect in producing improvement in all the parameters compared to both the groups. University of Kelaniya. University of Kelaniya. Anidra (Insomnia) is a condition of insufficient sleep with lack of quality and quantity. Tailadhara is an effective remedy. Asatmendriyarta Samyoga (unwholesome contact of senses with its objects) are mainly responsible for all the physical and mental diseases. Anidra PP 096: Effectiveness of Himajaladhara and Kumaripadma Snehadhara in the management of psychological stress with special refernce to ayurvedic Manobhava RP Liyanage1. Boralesgamuwa. University of Kelaniya. Cases were divided into 2 Groups of 10 individuals in each. The SA evaluated any changes or improvement in their feelings of physical and mental fitness. P Vitharana3 1 Gampaha Wickramarachchi Ayurveda Institute. Sri Lanka. Dhara karma at the Agnya Chakra are known as effective in psychological issues. The study was designed to compare the efficacy of Nidrakara effects of NS and SS. University of Kelaniya. Sri Lanka 3 Department of Cikitsa. Vatadosha is the primary responsible factor in Manasa Bhava (Objects of mind). 3 Department of Cikitsa. Keywords: Neelammahara. Stress is a crucial health issue worldwide and internal (drug) treatments for this cause various adverse effects. Neelammahara. NWGND Gamlath3.PP 095: Clinical efficacy of Neelakadi Snehadhara and Sathwawadi Taila Dhara in the management of Anidra with special reference to insomnia RP Liyanage1. According to Ayurveda. Pragnaparadha (intellectual error). Imbalances in Rajas and Thamas Guna also promote psychological stress. anxiolytic disturbances and Depressive thoughts. Sri Lanka. SS Hettige2. 75 % (P<0. The overall effect of therapy showed marked improvement of 82. Keywords: Manobhava.71 % (P<0. Dhara. Data were analyzed by Wilcoxon matched-pairs signed ranks test and inter-group comparison by Mann-Whitney Test. stress PP 097: A comparative clinical study on efficacy of Shodhana Karma and Shaman Karma in the management of Ekakushta (Psoriasis) MVR Wijayanthamala Gampaha Wikramarachchi Ayurveda teaching hospital Yakkala Sri Lanka Psoriasis is a global health problem. Psoriasis is comparable in Ayurveda with Ekakushta. Shamana PP 098: Management of varm e ma'ida (gastritis) with Makko juice (extract of Solanum nigrum) RF Rismiya. psoriasis. University Of Colombo. gastritis and other gastric problems. Only GR 1g twice a day and JY for external application for 1 month were administrated. Group C was subjected only with SPT.02 % in group B. Keywords: Ekakushtha. Group A were subjected to Himajaladhara and SPT.57 % in group A and moderate improvement was observed in 71. MLU Salma Institute of Indigenous Medicine. it is chronic in nature and cannot be easily cured. Vamana. To compare the Shodhana Karma and Shamana Karma in the management of Ekakushtha. Virecana. Sri Lanka Makko (Solanum nigrum Linn. In group A. Group B was subjected to Snehadhara and SPT. The results suggest that GR and JY can be used to manage Psoriasis without Shodana Karma when it is contraindicating to the patients. Snehadhara elaborated better results in Manobhava compared to the Himajaladhara. Pacification of provoked Vata using Snehadhara promoted the Medhya. Gastritis is inflammation of the 206 .0001) and statistically the result was highly significant in both groups. classical Vamana Karma followed by Virecana Karma followed by Gandhaka Rasayana (GR) 1g twice a day and Jivantyadi Yamaka (GY) for external application for 1 month were administrated. even if it is cured relapses are common. Harsha. Sthairya Mana. Group A showed 85. In group B. Data were statistically analyzed. 60 patients fulfilling the inclusion criteria were selected from the OPD of the National Institute of Ayurveda Jaipur and randomly divided in to two groups. All the 3 groups were found to have significant improvement. Inter group comparison showed better results in Group A than Group B but Group B also showed considerable results comparatively.was conducted for 21 consecutive days with 45 minutes of Dhara Karma and 30 minutes of supportive psychotherapy (SPT). Shraddha and Smruti.) commonly known as Black Nightshade is a dicot weed in the Solanaceae family. The assessment was taken at the beginning of the first day and at the end of the 21st day with the basis of the relief in the subjective and objective signs and symptoms of stress and Manobhava according to a grading system.0001) improvement in PASI score as compared to group B which had 81. The results were assessed on subjective criteria through PASI score. Leaves and berries of Solanum nigrum are commonly used in South India for the treatment of gastric ulcers. Chenbuk. "clinical application". Medline/ PubMed.417 ha. temperament PP 099: Status of Traditional Herbs Productions for Culinary and Medicinal Purpose in South Korea KW Park1. Keywords: Varm e ma'idahaar. Koreans are known to collect wild herbs for culinary and medicinal purposes. fresh root of Platycodon glandiflorum andfresh roots of Codonopsis lanceolata were main imported items. non- steroidal anti-inflammatory drugs (NSAIDs) (and aspirin) and autoimmunity. different vegetables(soybean sprouts. anti ulcerogenic. which is one of best Korean cuisine mixed with rice. in databases namely.157 ha. Isemyen. Korea Univerity. and caffeoylquinic acids are the main components of Aster. Only young leaves and root used for salad in spring while old leaves or roots in falls are used as medicinal herbs after drying. analgesic and ulcer healing properties that work on gastritis. Information of this review article was collected by searching for the key-words "Solanum nigrum". Wanjugun.Makko is cold in nature. but only 34 plants are cultivated for vegetable marketing while 32 are cultivated for medicinals. phytochemicals. it has anti. The three most important causes of gastritis are Helicobacter pylori infection. Daily intake of minerals. Pteridium. According to unani medical system Varm e ma'ida is an inflammation of the stomach which may be in Haar (hot). gastritis. Dried Osumudaart. Only 10% are cultivated in greenhouse.412 ha and Platycodon glandiflorum 1. vitamins and caffeoylquinic acids through Bibimbab were calculated using simulation method.Wild vegetable used are for every menu.Platycodon) and hot spices. 703 Galsanri. but mainly for making Bibimbab. Findings of this review show Varm e ma'idahaar is hot in nature.Cultivated areas of wild vegetables is about 11. Aster scaber 1. "pharmacological effect".195 ha. Keywords: wild vegetables. Pteridium aquilium 2. commonly caused by chronic irritation and inflammation. anti -gastric. Science direct. Korea 2 KFRI. anti - oxidative. The aim of this study was to overview the literature.Total value of imported wild vegetables was 45 mill. It is due to alteration in Damavi (sanguineous) and Safravi (bile) Humour. Seoul. S H Kim2 1 Division of Biotechnology College of Bioscience. Scopus. In Korea about 480 wild plants are edible. Google Scholar.inflammatory. caffeoylquinic acids 207 . Phytochemicals such as astersaponin. Baarid (cold) and Sulb (hard) in nature. authentic books and results of research studies on the pharmacological effect of Makko and its medicinal property on treating gastric inflammation. Korea Landscape in Korea is about 70% mountains and 30% arable land. From ancient times. As the result of this study it can be concluded due to its cold temperament and its pharmacological property Makko is a very good single herb that helps to cure Haar type Varm e ma'ida. chrysanthemaxantbin. dollar in 2015.gastric mucosa. and "animal studies". Aster. mushroom. dried Pteridium.000ha in 2011: Codonopsis lanceolata 2. specificity. Sri Lanka 2 Neurology Unit. There are eight health care institutions in TCM run by the central government. Sri Lanka 3 Radiology unit. Karolinska University Hospital. HMA Sominanda6.10 million). Faculty of Medicine. Sweden Recently. number of special clinic patients increased from 129940 to 198911 and annual expenditure incurred by these institutions in Sri Lankan rupees increased from 544828400 (544. Sri Lanka and Department of Clinical Neuroscience. even though the allocations and number of medical officers gradually increased in recent years. accuracy. Sri Lankan ayurveda. Keywords: TCM. Assessment and documentation of performance of the TCM system is vital for the development of health care for the nation. number of outpatients and inpatients. There is limited research regarding the applicability of these in Asia. Sweden 5 Department of Clinical Neuroscience. The indicators that were analysed to assess the progress were.83million) to 2573100000 (2573. Patients with clinically isolated syndrome diagnosed by 208 . Ayurveda. number of patients warded remained relatively stable from 56428 to 5768. Sweden 6 Department of Anatomy. Sri Lanka Traditional and Complementary medicine (TCM) dates back to ancient cultures and civilizations. A Fogdell-Hahn5. It was found that. Thus. performance PP 101: Which Magnetic Resonance Imaging criteria suits the diagnosis of Multiple Sclerosis in Sri Lanka: McDonald 2010 or Proposed MAGNIMS 2016? SMK Gamage1. number of TCM medical officers employed. P Wijesinghe3. University of Peradeniya. J Hillert5. Faculty of Medicine. 1 Department of Anatomy. This survey aimed to assess the performance of TCM services delivered by the central government ayurvedic hospitals in Sri Lanka in recent years. K Fink4. Karolinska Institutet. Teaching Hospital Kandy. It plays an important role in primary health care in many countries.PP 100: Traditional and complementary health care services through Central Government Institutions in Sri Lanka . patients’ turnover and annual expenditure incurred. Siddha and traditional medicines are different types of medical systems which are being practiced in Sri Lanka. the number of beneficiaries decreased. SB Adikari1. both criteria were developed based on Caucasians. University of Peradeniya. positive predictive value (PPV) and negative predictive value (NPV) of McDonald 2010 and MAGNIMS MRI criteria to verify their applicability in Sri Lankan patients. the performance of these institutions must be further evaluated to assess the cost effectiveness of the TCM services. Nevertheless. number of special clinics. I Wijeweera2. Karolinska Institutet. Unani. from 2012 to 2016 the number of medical officers employed changed from 186 to 232. Sri Lanka 4 Department of Neurology. The records of the department of Ayurveda from 2012 to 2016 were checked.A survey T Sivakumar Bandaranaike Memorial Ayurvedic Research Institute. to diagnose multiple sclerosis (MS). It was noted that. Peradeniya. Sirimavo Bandaranaike Specialized Children’s Hospital. The objective of this study was to determine sensitivity. number of patients to the out-patient department increased from 330659 to 448282. MAGNIMS group proposed guidelines to replace existing ‘dissemination in space’ criteria in McDonald 2010. MAGNIMS MRI criteria were fulfilled by 29. InsanJukYeom is baked 8 times with old pine tree wood at over 800 degrees centigrade. 96% respectively. Insan. as a versatile healthy food. terminal cancers. McDonald and MAGNIMS MRI criteria were applied to all MRIs. accuracy and PPV but inferior in sensitivity and NPV in this group. Keywords: MRI criteria for mutiple sclerosis. and Yeom. Sensitivity. toothache. specificity. CDMS was defined as at least two MS attacks and two clinically evident lesions. Keywords: bamboo salt. while Juk refers to bamboo. Insan-Kim YilHoon’s two true successors have now produced InsanJukYeom that is famous for popular food ingredients with medicinal properties. Dr. Dr. South Korean people widely eat and apply it on the body to heal diseases such as. the Korean bamboo salt. Institute of Indigenous Medicine. Data was collected by literature review and by observations. Sri Lanka 2 Department of Basic Principles. Patients were followed- up for 2 years to assess conversion to clinically definite MS (CDMS). Of 66 patients. various intractable diseases. Thirty seven fulfilled McDonalds MRI criteria. Sri Lanka InsanJukYeom. It also works for dogs and cats. who was a traditional Korean doctor. scar removal due to skin damage. 62 respectively. Sensitivities. PPV and NPV for prediction of conversion to CDMS were calculated. Of them. University of Kelaniya. Insan is the founder’s pen name. 42 converted to CDMS. Of them the majority (33) converted to CDMS. MAGNIMS is superior to McDonald in specificity. McDonald criteria. vaginitis. the Korean bamboo salt is made only in South Korea. The aim of this study was to review InsanJukYeom. hair loss. atopic dermatitis. MAGNIMS criteria PP 102: A Review of InsanJukYeom. The study has revealed that InsanJukYeom presently has been consumed as a healthy food for various disease conditions and used as an effective edible food for health promotion in Korean community. Insan developed this new material InsanJukYeom.consultant neurologists were recruited from five main neurology centres. Baseline MRIs and follow- up MRIs were performed within 3 months from initial presentation and one year from baseline MRI respectively. The temperature reaches somewhere between 1300 to 2500 degrees. Insan-Kim YilHoon (1909-1992). gastritis. Accuracies of McDonald and MAGNIMS were 64 and 77 respectively. University of Colombo. 28 converted to CDMS. accuracy. McDonald and MAGNIMS had PPV. and then it is roasted one more time with pine resin only. NPV of 89. InsanJukYeom. In conclusion. means solar salt. The raw materials of InsanJukYeom are solar salt which is only collected from the west coast of Korea and bamboo known as Phyllostachysbambusoides Siebold and Zucc which is only found on the south coast of Korea. MWSJ Kumari2 1 Postgraduate Institute of Pali and Buddhist Studies. 69 and 96.Kim YilHoon 209 . 83% and 67%. etc. specificities of McDonalds and MAGNIMS were 78%. multiple sclerosis in Asia. as a versatile healthy food SY Lee1. which was turned into mineral chunks that completely eliminate the toxicity of salt. random observations were done with respect to BHS guidelines techniques on measuring blood pressure. Maharagama and Matara with 100 Ayurveda physicians. He wrote in Liber Al-Mansuri (Kitab al-Mansuri) that physicians are happy to treat his patients with diet and food without drugs. periodic functional evaluation of BP apparatus. "Drinks and foods" is a key issue in the six essential principles (Seteyhzarooriyeh) for keeping health which has a noticeable outcome for the protection of health and management of illnesses. Without acknowledgement. Gorgan. It is composed of systolic and diastolic blood pressure. wrong diastolic endpoint (K4 or K5). Suggestions to improve BP measurement in Ayurveda physicians are. identify problems and issues in pressure measuring. Data analysis was done according to the 10 criteria’s and standers analysis. The objectives of this study were to. which corresponds to the pressure following contraction of the heart and pressure during relaxation of the heart respectively. The major errors observed included. Attention to nutrition is the first step for maintenance of wellbeing and treatment in PM. Sri Lanka Blood pressure is a vital sign.Moloki) that food will cure as much a doctor. In Persian medicine (PM) this topic is very significant and PM sages utilise preventive recommendations. School of Medicine. Data collection was done at Colombo. evaluate pressure measurements. There are many scientists and books with great experiences in the field of 210 . and regular inspection and availability of BP monitors with relevant cuffs. University of Kelaniya. Iran Doctors in current medicine emphasize prevention much more than treatment. rapid cuff deflation and single one off reading.PP 103: Clinical governance in Ayurveda physician’s blood pressure measuring techniques WHAP Senanayake Gampaha Wickramarachchi Ayurveda Institute. School of Medicine. Mercury column not level with the eyes. Keywords: blood pressure. There are many methods for maintaining health and treatment in PM literatures. prior to implementation. BP measurement by Ayurveda physicians shows good performance. clinical governance. Considering total observations. regular higher level guidance to junior staff. except for a few correctable issues. Persian medicine is one of the schools of medicine used from centuries ago up to the present time as a prevention measure and as a method of diagnosis of diseases and managing the patients. Normal blood pressure should be around 120/80. Rhazes (865-925 AD) is one of the famous physicians in PM that focus on beginning treatment with diet. Ayurveda general practice PP 104: Importance of nutrition in prevention and treatment in Persian medicine Marzieh Qaraaty Metabolic Disorders research center. failure to hear the Krokoff sounds. Iran and Department of Persian Medicine. He recommended Simple and Compound medicines for treating at the next levels. new BP apparatus should be compared with the standard monitors. Golestan University of Medical Sciences. reflecting the pressure exerted on blood vessels when blood is forced out of the heart during contraction. Instrumental factors and Procedure factors. Gorgan. set criteria and standards and suggest possible solutions. Human factors. This article is a review of the importance of nutrition in PM. under 3 categories. Gampaha. He also wrote in another book (Tebe. Golestan University of Medical Sciences. 400. NZ Iqbal2.. BM Nageeb1. Studies reveal that some micronutrients are essential to control excess weight. Nawaloka Hospital (Pvt) Ltd. Keywords: nutrition. BM Nageeb1. Despite excessive dietary consumption. Then the mixture was kept in the dark at room temperature (30oC) for 15 minutes. Researchers have revealed that excessive oxidative stress on tissues is one of the main problems in obesity and anti-oxidants play a key role in regulation of excess body weight.25 mM DPPH (40 µL) was added to each well of the micro plate which contains different concentrations of the extracts. Butylated Hydroxy Toluene (BHT) was used as the standard. separately. Sri Lanka Globally obesity is increasing alarmingly. The absorbance was measured using a spectrophotometer at 517 nm wave length. Sri Lanka 3 Department of Chemistry. The spice mixture contains 5 spices commonly used in Sri Lanka: Cinnamomunzeylanicum. ‘spice’ mixture. obesity PP 106: Determination of mineral contents in a native ‘spice’ mixture used to reduce excess weight MI Manuha1. These provide tips that can be used for by all educational and therapeutic centers. University of Kelaniya. The aim of the study is to determine mineral contents in a native ‘spice’ mixture that was used to reduce weight in excess weight individuals. 50. Cuminumcyminum. Diphenyl -1. 211 . Murrayakoenigiiand Alliumsativum. Sri Lanka 2 Dietician. A 0.05) with the percentage inhibition of the standard BHT. The objective of this study was to determine the anti-oxidant contents in a native ‘spice mixture’ that is used to reduce excess body weight. University of Colombo. prevention. PA Paranagama3 1 Institute of Indigenous Medicine. PA Paranagama3 1 Institute of Indigenous Medicine. The study concludes that ‘spice’ mixture can be used to reduce excess weight as it potentiated substantial anti-oxidants. University of Kelaniya.oxidant activity of native spice mixture used in weight reduction by DPPH radical scavenging assay MI Manuha1. Sri Lanka 2 Nawaloka Hospital (Pvt) Ltd. A known volume (160 µL) of each concentration (25. treatment PP 105: Determination of anti. 200.. The spice mixture contains 5 spices commonly used in Sri Lanka such as Cinnamomunzeylanicum. Piper nigram. 800 µg / mL) from both samples (aqueous and methanol) were pipetted out to a 96 well micro plate. University of Colombo. 100. Sri Lanka 3 Department of Chemistry.treatment and prevention in PM that are unknown. NZ Iqbal2. studies say high rates of micronutrient deficiencies are also one of the causes for obesity. Keywords: anti-oxidant. Sri Lanka Obesity is one of the current global problems.picrylhydrazyl (DPPH) radical scavenging activity assay was used to determine the anti-oxidant potential of the ‘spice’ mixture. Methanol (MeOH) extract of the ‘spice’ mixture. Dilutions were prepared by aqueous. Persian medicine. The study revealed that the ID 50 of the aqueous and methanol extracts of the spice mixture are not significantly different (p < 0. 8 mg / kg).15 mg / kg respectively. Piper nigram. In addition. Sri Lanka 2 Nawaloka Hospital (Pvt) Ltd. Since it has been reported that Fe. there is a need to determine the exact form in which these minerals and metals are found in the formulation. University of Kelaniya. It was revealed that Potassium content was 1.3 mg / kg. Selenium and Magnesium were present in trace amounts with the values 0. Calcium.69. As biomarkers.33. Murrayakoenigii and Allium sativum. Zn. 0. cobalt was not detected. Before intervention. BM Nageeb1. Also other obesity related factors were recorded in the study. Selected female volunteers (n=97). The biomarkers and obesity-related symptoms recorded before and after the intervention showed a significant difference (p < 0. Murrayakoenigii and Allium sativum.70. mean of the BMI was 32.Cuminumcyminum. SGPT. Keywords: overweight. The element. Determination of the effectiveness of the intervention of a ‘spice’ mixture in the management of female overweight/obesity is the main objective of the present study. The study revealed that Iron content in the spice mixture was the highest (72.11 (2006) method by using Atomic Absorption Spectroscopy. obesity. Further studies are needed to clinically validate the efficacy of this product. Se and Cu are essential to reduce excess weight.9 kg / m2. while Zinc holds the second place (24. Piper nigram.7 mg / kg). After the intervention it was 30. The spice mixture contains 5 spices commonly used in Sri Lanka such as Cinnamomunzeylanicum.05) and the SGPT and SC levels of the study group indicate that the liver and kidney tissues were not impaired by the ‘spice’ mixture. The sample was placed to a muffle furnace and the ash obtained was dissolved in 6 M Hydro Chloric-acid (HCl). 0. lipid profile. serum creatinine (SC) and the existence of obesity-related symptoms were recorded for all the participants before and after the intervention. and reduce sedentary behaviors to diminish the growing obese population. Keywords: micronutrients. Cuminumcyminum. Hb % . Nine minerals were detected according to the Association of Analytical Communities (AOAC) 999. The findings emphasize a need for implementing a proper intervention to promote healthy eating habits with ‘spice’ mixture.2 kg / m2 and the mean change was 2. encourage physical activity. Sri Lanka Obesity and overweight lead to non-communicable diseases such as high cholesterol. aged between 18-55 years were recruited in this study. NZ Iqbal2.. Approval for the ethical clearance was taken. coronary heart disease and type 2 diabetes mellitus. Sri Lanka 3 Department of Chemistry. ‘spice’ mixture 212 . PA Paranagama3 1 Institute of Indigenous Medicine. it can be concluded that the spice mixture can function as a mineral supplement. University of Colombo. Chromium.17 and 0.5 mg / kg) followed by Copper (8. spice mixture PP 107: Efficacy of the intervention of natural food additives (a ‘spice’ mixture) in the management of female obesity MI Manuha1. obesity. where formula 671 showed the best results. sample 671 was selected for the final product development. Two thirds (78%) were female. flavour. Results were analyzed using MINITAB 14 for Kruskal Wallis non parametric analysis and Mann-Whitney test. Study population consisted of 100 patients of both sexes between 18 -81yrs. Institute of Indigenous Medicine. health. sensory evaluation 213 . there was no statistically significant differences among BMI and Dinacharya (p >0. Considering the overall results of three sensory evaluations. Sri Lanka In Ayurveda. Overall more than one fourth was following selected steps of Dinacharya. carrots and garlic were dehydrated and powdered prior to using as ingredients. This is a community based descriptive cross sectional study carried out among the participants who participated to the mobile clinic at Dondra. Based on the results of two sensory evaluations. The study tool was a self-administered questionnaire designed on various aspects of Dinachariya. Faculty of Applied Sciences. University of Colombo. WMSSK Kulathunga Department of Swasthavritta. carrot and garlic along with the least amount of mushroom compared to other samples Keywords: Mono Sodium Glutamate. MAJ Wansapala Department of Food Science and Technology. Ayurveda PP 109: Formulation of a natural and nutritious flavor enhancer by using locally available ingredients having umami taste KN Wijayasekara. This study supports promoting Ushapana and taking main meals on time to enhance health status. as a replacement for Mono Sodium Glutamate.1. excluding pregnant mothers and children. Ushapana compared with morbidity was also significantly associated (p= 0. The present study was conducted to identify the effect of daily routine on health. The mean estimated BMI + SD for the studied sample was 24. However. Formulations were added into dhal curry and sensory tests were carried out with dhal and bread. 37% were overweight and the prevalence of overweight was higher among females (28%) compared to male (9%). taste. Sri Lanka The main objective of this study was to test a formulation of a natural and nutritious flavour enhancer using locally available ingredients having an umami taste. Thirty untrained panelists participated in the sensory evaluation and samples were analyzed for appearance. odor. “Dinacharya” is considered as an important concept for maintaining health. Keywords: Dinachariya. tomatoes. These were mixed according to a Taguchi L8 design. sample 767 and 671 were selected for the third evaluation.05). Out of all the samples. University of Sri Jayewardenepura. Mushrooms. There was a statistically significant association between taking main meals on time with morbidity (p= 0. ridding the body of diseases and maintaining the body in Tridosa balanced state in a healthy person.94.55 + 4. selected formula (tomato 2: mushroom 1: carrot 2: garlic 2) contains the highest level of tomato.033).043). The mean age of the sample was 52. More than half of the studied sample (61%) had normal BMI. mouth feel and overall acceptability.PP 108: A study of the relationship between daily routine (Dinacharya) and health BL Edirisinghe. More than one third of the studied sample (40%) consumed a healthy balanced diet.25 + 14. Eight different formulations were evaluated based on a seven point hedonic scale. hypertension (24%). It is fuelled by an unhealthy diet. Sri Lanka 2 King’s Mill Hospital. maldive fish etc. The most common diseases were musculoskeletal complaints (57%).2 times / week.14±4. Sutton in Ashfeild. These drinks and food could increase blood glucose concentration. soft drink was consumed by all diabetics. with BMI to Fenugreek (p=0. region. Sri Lanka 4 North Matale Hospital. clove. Matale. etc.2 times / month.6. WMSSK Kulathunga Institute of Indigenous Medicine. these drinks and diets are highly processed.) spices (cinnamon. tastes and habit of the people. The objective of this study was to study Sri Lankan cuisine’s effect on health. University of Colombo.They used both traditional and modern cooking tools.54 respectively. They used both traditional and modern flavor enhancers.39±14.) and cooking fuel (wood etc.3 % and 56. unemployment and high intake of salty foods (dry fish. In this study male and female diabetic patients were 43. 24.1 ± 3. Obesity. SM Mujahid4 1 Institute of Indigenous Medicine University of Colombo. They mainly used coconut oil (88%) and coconut milk (87%) as the cooking media. and with hypertension to coconut oil (p-0. Often. KA Naser2. 1 time / day. etc. There was a negative correlation. traditional cooking tool (clay pot. BM Nageeb1. customs. (chilli. 40% of those studied were Obese. The aim of the study was to determine the relationship between consumption of soft drink and diabetes control. Keywords: Sri Lankan cuisine. etc. A total number of 59 controlled and 61 uncontrolled diabetics were recruited for the study.043). The age of patients varied from 20 to 70 years. Sri Lanka Sri Lankan traditional food and drink are being influenced by western diets and drink. With the presence of past food establishments. gastritis (21%) and diabetes mellitus (19%).4 Kg/m-2.7 % respectively. non-communicable diseases PP 111: The relationship between consumption of soft drink and diabetes in selected areas of Central province AM Muthalib1.5 times / day and 6 times or more/ day. According to the result. 1 . No patient reported as never 214 .).039). obese. climate.039). The frequency of consumption was grouped as never. with a high glycemic index and high glycemic load.). with hyperlipidemia to Sweet cumin (p=0.032) and Gamboge (p=0. Nottinghamshire.000) and coconut oil (p=0.) are common correlations with the diseases among the group. R Sivakanesan3. There was a positive correlation with skin diseases to vinegar. In the present study. 1. Cuisine varies widely with the kind of food product. United Kingdom 3 Department of Biochemistry. University of Peradeniya. salt etc. Further studies are recommended for investigating Sri Lankan cuisine for the society to improve their health status. The mean BMI was 26. Faculty of Medicine. Sri Lanka Non-communicable diseases are a serious public health issue in Sri Lanka. religion. 2 . Sri Lankan still prepares traditional dishes for consumption like the use of various traditional cooking media (coconut milk and oil. maldive fish. mean value of age and BMI level were 51. 100 patients (15 to 75 year old females) from an Ayurveda mobile clinic at Gandara were interviewed by using a questionnaire. economic capacity.PP 110: A study of effects of Sri Lankan cuisine on health AB Dharmarathna.). flavor enhancers. Increased consumption of soft drink was observed in uncontrolled diabetics than controlled diabetics. raw and PC pulses. 66. Sri Lanka The official part of Heterophragmaadenophyllum (Bignoniaceae). Trolox. Keywords: diabetics. ascorbic acid and Fe2+ standards respectively. total phenolic content (TPC). 33. Data was analyzed using Matlab 7. gallic acid.0 (R2010a). The t-test shows there are significant differences at p<0. 28.05 in flavonoid content of OC and PC fruits. OC and PC vegetables and raw and OC green leaves. Significantly higher antioxidant activity and flavonoid content were observed in PC foods when compared to OC foods. Bandaranaike Memorial Ayurveda Institute. Raw foods showed superior flavonoid content and antioxidant activity when compared to OC foods.3%. OC and PC roots. RK Jayaratne Department of Ayurveda. Keywords: food. India Bioactive potentials of foods are generally estimated in raw forms.5%. bioactive PP 113: Macroscopic and microscopic identification of root and stem of Heterophragma adenophyllum (Palol) JM Pushpalatha. The flavonoid.4% in 1 time/day and 40%. More research has to be done on this in the future. 71. But there were no significant changes in TPC among food groups inspected to the processing methods except OC and PC green leaves. open cooked (OC) and pressure cooked (PC) forms. Therefore. Sri Lanka 2 All India Coordinated Research Project on Nutrition. antioxidant activity.5% in 1 . EAB Edirisinghe. R Kamalaja2 1 Institute of Postharvest Technology. raw and OC vegetables.2 times / month. raw and PC roots. 60% in 2 – 5 time/day groups. glucose level PP 112: The effects of processing methods of bio-active properties of commonly consumed food products S Thiruchenduran1.6%. Department of Foods and Nutrition.10. This study suggests that soft drink consumption has a strong effect on blood glucose level. flavonoid. 47.consuming. It was found that the market sample of Palol is a mixture of roots and mostly stem pieces. Bioavailability of the functional compounds may depend on the processing methods. This study explores the effect of the flavonoid content. The habit of soft drink intake in controlled and uncontrolled diabetics were 52. Professor Jayashankar Telangana State Agricultural University. respectively. soft drinks. total phenolic content and antioxidant activities of selected foods in raw. Hyderabad. raw and OC spices and OC and PC vegetables.Palol is mentioned as a root which is among Dashamoola group of herbs which is important in the management of various diseases. National Aquatic Resources Research and Development Agency. Considering the results it can be concluded that the loss in flavonoid and antioxidant activity in foods are higher in open cooking than pressure cooking. DPPH radical scavenging activity and FRAP value was evaluated by standard methods using rutin. No patients reported drinking 6 times or more / day. 215 .7% in 1 – 2 times / week. TPC was not influenced by any of the above cooking methods. Significant antioxidant activity difference was observed among PC and OC fruits. Market samples of this drug are often adulterated with their aerial parts. is a significant feature in shoot while such arrangement is absent in root. Keywords: Palol.anatomical features. which has white wood and a rough outer surface. in between cork and the xylem region separating fibre patches. Root wood is diffuse porous while shoot wood is semi ring porous. macroscopy. Large circular starch grains are abundant in root cortex while they are comparatively small in size and number in shoot cortex. Anatomically it is notable that the root cork is wider than (20-40 layers) the stem cork (15-25 layers). the root could be identified by its wood color (yellow). Macroscopically. outer surface (furrowed) and odor which is a little stronger than the stem. morpho-anatomy 216 . Therefore. Bandaranaike Memorial Ayurveda Institute. Results revealed that these pharmacognostic features could be used in authentication of the raw material of Heterophragma adenophyllum (Palol). Presence of parenchyma (devoid of starch grains) in longitudinal strips. But both parts are almost similar in internal colour (creamy white). The result shows the root of Aegle marmelos could be easily identified from its shoot using above morpho. root. Sri Lanka The root of Aegle marmelos (Rutaceae) is an important drug due to its wide range of therapeutic actions. Comparative anatomical study showed. Xylem pores in the stem are mostly oval and arranged in a diffuse porous manner with pores solitary and in short radial multiples while they are circular and arranged exclusively solitary in roots. Externally. cork (10-30 cell layers) and the bark region are relatively thick in root than in shoot (5-20 cell layers of cork). microscopy PP 114: A comparative study of morpho-anatomy of root and shoot of Aegle marmelos (Beli) RK Jayaratne. The pore shape is mostly oval in the stem while it is mostly circular in the root. it is necessary to highlight the morpho-anatomical features of the root and shoot of this plant for proper identification. Significant aromatic odor and astringent bitter taste is specific more in the root than in shoot. the root can be identified by its yellowish brown colour and comparatively less ruptured surface. stem. root. shoot.botanical pharmacognostical investigation was carried out to differentiate roots from its stem pieces. Although sclerenchymatic bands are prominent in the xylem region of the root they are not obvious in the same region of the stem. A comparatively large irregular pith consists of thin walled parenchyma cells which is detectable in the stem but absent in the root. organoleptic and microscopic evaluations were carried out according to WHO guidelines. The stellate shaped pith consists of numerous starch grains and is distinct in the shoot while the pith is negligible in the root. EAB Edirisinghe. Both parts of the same plant were obtained and morphological. Macroscopic and microscopic studies of root and stem of the same plant were carried out according to WHO standard methods. JM Pushpalatha Department of Ayurveda. Keywords: Aegle marmelos. Phelloderm is clear in the stem whereas it is not significant in the root. Bulu (Terminaliabellirica) and Nelli (Phyllanthusemblica) was selected for the study. Vanishing cream -V2 and All-purpose cream V3 both made up of Varnya drugs which was in Kumkumadi Taila Kalka Dravya and to compare the results of Taila and cream.01). group 2 – V1. Varnya cream (V) . Sri Lanka Cosmetology is the science of beautifying skin and its appendages. post harvesting.5×10⁴ cfu/g. Cold cream works better then all-purpose cream. containing fruits of Aralu (Terminaliachebula). Due to its unique concept and therapy regarding beauty and health. Colombo and Kurunegala areas were assayed for their microbial quality using the pour plate and spread plate technique.57 ×105 cfu/g to 3. group 3 -V2 and group 4 -V3 were given for 1 month as an external application. V3 also worked well on Varnya effect with a relief percentage of 46. Kumkumadi Taila. Keywords: herbal raw materials. Plates were subjected to calculation of the erythematic means of the No. University of Kelaniya. of colonies in terms of cfu/g. Taila and cream which were prepared with Varnya drugs has shown medicinal effect with skin complexion. and Nelli from the herbal raw material outlets in Gampaha. RMW Liyanage1. Bacterial count of Aralu varies from 1. microbial count 217 . Yeast and mould count were recorded as Aralu 2×10⁴ cfu/g to 4×10⁴ cfu/g. safety and efficacy of herbal medicine is significant.2×10⁴ cfu/g. V2 gives 40% relief and (p<0.66% (p<0. Taila therapy is better than cream therapy.22 ×105 cfu/g and Nelli from 2.71 ×105. it is now time to establish a special branch of cosmetology in the field of Ayurveda.Cold cream form made of Kumkumadi Taila -V1. Widely used herbal raw material Tripala. WMB Weerasooriya2 1 Department of Cikitsa. Gampaha Wickramarachchi Ayurveda Institute. Group 1 –Kumkumadi Taila. Von Luchnan Scale PP 116: Quality assessment of raw materials ofTripala GM Rukmalee1.8×10⁴ cfu/g to 3.6×10⁴ cfu/g to 4. Sri Lanka 2 Department of Dravyagunavignana.1×10⁴ cfu/g and Nelli 2.PP 115: A comparative clinical study of Varnya drugs in different formulae on skin complexion MVR Wijayanthamala Gampaha Wikramarachchi Ayurveda Teaching Hospital. Randomly collected dried Fruits of Aralu. University of Kelaniya. The results revealed that the microbial count of tested samples exceeded the WHO recommended microbial count. Evaluation of microbial quality of selected raw materials was the objectives of this study. Tripala. Bulu. which in turn works better than vanishing cream. This field of cosmetology is increasing at a rapid rate.01). Bulu 1.8×10⁴ cfu/g to 6. Since Vedic times we see a conscious effort of man towards looking beautiful. The raw materials of herbal medicine are subjected to contamination with micro-organisms during the cultivation. Microbial quality has been identified as a parameter in standardization of herbal products. Gampaha Wickramarachchi Ayurveda Institute. Sri Lanka Evaluation of quality.01) shows significant results. processing stages and storage. Effect of the therapy as per Von Luchnan Scale on KumkumadiTaila and V1 were found on skin complexion with maximum relief of 53. Bulu varies from 2.33% (P<0. microbial quality. Keywords: Varnya.11 ×105 to 3. This study was conducted to assess the Varnya (complexion) effect of Kumkumadi Taila. harvesting. Sixty dark skin volunteers were selected and randomly divided into 4 groups. Making necessary arrangements for awareness of tourists about the benefits of this Ayurvedic therapy is important. journals and other published materials related to the study. cough. University of Colombo. saponins. Keywords: Abhyanga. total ash. P Galappatty2. guidelines. most suitable times to do the massage therapy. Further phenols. Finally it is important to widen health tourism to increase the economy of the country. University of Colombo. steroids and terpinoids and steroids were more prominent in both plant extracts.) Moon (Bharngi) in the Ayurveda system of medicine JM Dahanayake1. Hence in this research an attempt was made to investigate the possibility of the usage of Siritekku as a substitute for CS. phytochemical constituents and TLC fingerprint patterns. terpenoids and cardiac glycosides in two samples. rules and regulations on SPA according to national and international levels were studied using the above books. Proper methods of Ayurvedic massage therapy. physico-chemical (extractable matter including both hot and cold extracts of water and ethanol. 218 . Sri Lanka Clerodendrum serratum (L. tourism PP 118: Investigation of the possibility of using Premna herbacea (Siritekku) instead of Clerodendrum serratum (L. for a long time. tannins. Sri Lanka 2 Ayurvedic Teaching Hospital. Phytochemical screening revealed the presence of phenols. services and entertainment for people who are visiting a place for pleasure. PK Perera1. massage. Considering this data it can be concluded that using Ayurvedic massage therapy can develop the physical well-being and relaxation of the people as both preventive and curative therapy. suitable herbs to use within the therapy. fever and other inflammatory diseases. In the Sri Lankan market. Faculty of Medicine. Sri Lanka Tourism consists of a wide range of business activity providing accommodation. This study aims to asses the benefits of Ayurvedic massage therapy /Abhyanga. with some controversial views existing about the identification of Bharngi. The data was mainly gathered from reading books Wruddaththraya (Caraka Samhitha. In Ayurveda. Sri Lanka 3 Industrial Technology Institute. University of Colombo . The root powder of CS and Siritekku were evaluated for pharmacognostical. is to be done with suitable rules and regulations. saponins. water soluble and acid insoluble ash). flavonoids. Removal of the misperceptions of locals. Bharngi). Institute of Indigenous Medicine. examine the guidelines. LDAM Arawwawala3 1 Department in Dravyaguna Vignana. Sri Lanka 2 Department of Pharmacology. rules and regulations on SPA according to national and international levels and to propose a plan of action based on the result of the study. Ayurvedic treatments provide a number of benefits to travelers by making physical and mental wellness. it is mainly used for the treatment of asthma.) Moon (CS) is an important medicinal plant distributed throughout the forests of India and Sri Lanka. the plant material called “Siritekku” has been used as the roots of CS (San. about the therapy.PP 117: A study on the efficacy of using Ayurvedic massage therapy in tourism industry AAKL Perera1. benefits of Ayurvedic massage therapy. EVP Hemachandra2 1 Institute of Indigenous Medicine. In India also many drugs are available in market with the name of Bharngi. steroids. P Fernando3. Susrutha Ssamhitha an Ashtangahrdya Samhitha). usage. Watika. Lepa. poorly contributed to knowledge and labeling architecture was did not create consumption stimuli. Keywords: Bharngi.4% of product brands did not assure consumer protection.However. Arishta. color. consumer intelligence and consciousness on labeling information and also identified the relevant consumption stimuli. Asawa. University of Sri Jayewardenepura. in Sri Lanka.7% of product brands had ISO 9001:2008. ingredients. Quawatha. the present findings justify the suitability of using siritekku as a substitute for CS. Physico-chemical parameters such as extractable matter and ash values were significantly higher in CS than in that of Siritekku. 44. Faculty of Applied Sciences. ISO22000. substitute. Sri Lanka Labeling. Sri Lanka Edible seaweeds have been consumed and used in Traditional Medicine for centuries by East Asian people. year of company establishment. Rasayana were considered. In conclusion. company contacts. HACCP. Gugggul. Leha. Some differences were observed in TLC fingerprint patterns and pharmacognostical studies between raw materials of CS and Sirithekku. I Wickramasinghe. However. authenticity and assay. product name. OHSAS. cardiac glycosides were present only in CS roots and alkaloids were present only in Siritekku roots. Faculty of Agricultural Sciences. these seaweeds are underutilized and they have potential to develop novel nutraceutical food products. traditional medicine. logo/symbol. consumer consciousness and consumption stimuli was high compared to the labels of small scale manufacturers which appears with limited information. Ayurveda medicine PP 119: Labeling architecture and consumer intelligence: Implications for consumer protection of traditional medicinal products BMRL Basnayake. printed or graphic matter upon the immediate container of an article is very important to a medicinal product because it is oftentimes a regulatory deliverable. Syrup. Sri Lanka PP 120: A value-added nutribar made from underutilized green seaweed Ulva lactuca from Sri Lanka RMAC Udayangani. A mixed methods approach was instrumental to collect the data and 62 brands of traditional medicinal product typology. Kalka. MA Jayasinghe Department of Food Science & Technology. Four species of edible green seaweeds (Ulvalactuca. Siritekku. DAM De Silva Department of Agribusiness Management. Modaka. health benefits. Information architecture of the labels positioned the established brands in a higher position in the brand positioning map. Brand recall memory. This study focused on labeling and information architecture of traditional medicinal products produced in Sri Lanka. Choorna. GMP or registration number of Department of Ayurveda (40%) which confirms the credibility of products to customers on purity. Keywords: labelling architecture. WMTB Weddagala. I Wijesekara. and company contacts. usage/ dosage. Thaila. Results found that 17. Rasa. Sabaragamuwa University. a display of written. 219 . Key considerations of the label architecture were certification. except for colour. Our previous studies show the hypoglycaemic activity of the flower and the stalks of A.62% and 1. The total phenolic content of hot and cold aqueous extracts were also determined according to the method of Folin-Ciocalteau and calculated as gallic acid equivalents (GAE). physiochemical. their textural properties. University of Sri Jayewardenepura. crude protein contents and antioxidant activities were investigated. the U.6% respectively. flavanones.25 ± 0. Results showed that U. The parts of A.2% and 13.17 ± 0.37 ± 0. traditional medicine.9% of water soluble ash in the powder. Chaetomorphaantennina (16. TS Suresh3 1 Department of Allied Health Sciences. Faculty of Applied Sciences. WJABN Jayasuriya1.07%) followed by Caulerparacemosa (16. crispus were carried out as per WHO guidelines of quality control methods for medicinal plant materials. Further. lactuca in Sri Lanka can be processed to develop novel healthy and nutritious foods.2 mg GAE/g respectively. The crude protein contents of the above powdered seaweeds and proximate composition of U. compared to the control (0%).35%).Ulvacompressa. Nutribar PP 121: Physiochemical and phytochemical analysis of Aponogeton crispus R Sarveswaran1.90 ± 0. University of Sri Jayewardenepura. cereal-based nutribars incorporated with 0. lactuca showed significantly (at p ≤ 0.08%).05) the highest crude protein content (20. and 10% of dried U. The mineral and crude lipid contents of U. and Ulvacompressa (7. Dry matter and the moisture content of A.8% of total ash and 92. Interestingly. crispus. Faculty of Medical Sciences. lactuca were determined by Kjeldhal and AOAC methods. Under-utilized U. crispus which were standardized in the present study will be useful for the correct identification and authentication of this medicinal plant and to prevent this plant from adulteration. and Chaetomorphaantennina) were manually collected from the Southern Coast. Standardized flowers and stalks of A.69 ± 1. Sri Lanka. crispus were found to be 75. alkaloids. In addition. respectively. Matara. crispus was 4. crispus reveals the presence of 18. the total phenolic content of cold and hot aqueous extracts of A. IU Kariyawasam2. Preliminary phytochemical screening and physiochemical analysis for the aqueous extracts of A. phytochemical 220 .9 mg GAE/g and 69.3% of extractive values respectively. lactuca (w/w) were developed. crispus yielded 21. Faculty of Medical Sciences. Furthermore. proteins. Use of the flower and the stalk as a functional food is limited due to a lack of information regarding standardization. University of Sri Jayewardenepura. Keywords: seaweeds. respectively.13%). Results show reducing sugar. amino acids. crispus using preliminary phytochemical screening and physiochemical analysis. Keywords: Aponogetoncrispus. 5. Hot and cold aqueous extracts of A. lactuca 5% enriched nutribar was shown almost similar in sensory and textural profiles. promoting seaweeds utilization will improve the life style of coastal families by generating an additional income. Caulerparacemosa. Sri Lanka.16 ± 1. flavonoids and polyphenols were found as phytoconstituents in both hot and cold extracts. Hence this study aims on pharmacognostical standardization of the flower and the stalk of A. crispus have many phytoconstituents.05%. lactuca were 17. Sri Lanka 2 Department of Botany. Moreover. 3 Department of Biochemistry.6% and 21. Physicochemical investigation of A. Ulvalactuca. Sri Lanka Aponogeton crispus is a fresh water monocotyledon belonging to genus Aponogeton and shows various medicinal properties. Keywords: Indralupta. Keywords: standards. S Kodikara Bandaranaike Memorial Ayurveda Research Institute.Indralupta has included under kshudra roga and as well as kapala roga. 50 balers/exporters and 30 value added producers. Belihuloya. Olinda (Abrus precatorius). Value chain analysis based on mixed method approach and the sample profile was 675 growers.PP 122: Standards and Certifications: a New Form of Marketing Tool . one of the first traded spices of the ancient world. Loss of hair occurs due to aggravation of pitta and vata and hair does not grow again due to obstruction of hair follicle from kapha and shonita. Majority of these herbals are mentioned under upa-visha drug category in RasaShashtra and comprised of Theekshna and Ushnaguna. Sri Lanka Ceylon cinnamon. cosmetics and perfumes were the main global market segments. AMIP Abeysinghe. 220 collectors. 485 peelers.A Case of the Ceylon Cinnamon Industry IC Hettiarachchi. Sri Lanka Indralupta can be co-related with Alopecia in modern medicine and “udugowwa kema” in traditional medicine. So it is very important to develop a proper treatment modality to treat Indralupta using indigenous knowledge as it is not having a proper cure yet. So it is very important to find out different drug formulas used in different parts of Sri Lanka to treat indralupta effectively. Results revealed that 52 % of the upstream actors were maintaining strong level of compliance on standards and certification. Daluk (Euphorbia antiquorum). Upstream actors of the cinnamon value chain have weak compliance level on quality standards and certification compared to downstream. 26 books were included to present study. Niyagala (Gloriosa superba). Lack of traceability and improper pricing mechanism hinder the compliance levels of the upstream while price premiums. external applications 221 . chemical and aromatic properties. Those recipes are different from one another in order to mostly available drugs in those areas in Sri Lanka which the book was written. Cinnamon Value Chain PP 123: A literature rveiw on indigenous external applications used in Indralupta Chikitsa MGDN Balasooriya. Objectives were to investigate the compliance of Ceylon cinnamon value chain members on quality standards (GMP/HACCP/ISO) and certification (Organic/fair-trade/FDA/BRC) and certificates and standards as a promotional tool. Exporters and value added producers used the standards and certification as strong marketing tool to enter into the high end markets and their labeling architecture allocated priory place for the standards and certification logos. is renowned for its medicinal. None of the cinnamon growers were certified for good agricultural practices and limited number of cinnamon processing factories was certified for good manufacturing practices. Mostly available herbals in those recipes are Rasakida (Tinospora cordifolia). bakery and confectionary. WDCK Karunaratna. herbo-mineral and mineral drug recipes are mentioned in Sri Lankan indigenous books. In Ayurveda. Faculty of Agricultural Sciences. M Esham Department of Agribusiness Management. 12 were herbo-mineral and 8 recipes were mineral. DAM De Silva. There are many effective herbals. Pharmaceutical. Indigenous medicine. Elabatu (Solanum indicum). Among them 52 recipes were herbals. competitive rivalry. certification. Welmee (Glyzerisa glabra) and makunuwenna (Alternanthera sessilis). brand loyalty attracts the downstream actors. 72 drug recipes were found out. National Aquatic Resources Research and Development Agency. Mean value of the ash content was 5. Faculty of Medicine and Allied Sciences. Putatively superior supercritical extraction (A) has been compared with Soxhlet’s extraction (B) using cinnamon oleoresin and the water extract (C) of cinnamon bark for their antioxidant activities. Faculty of Technology. P Kahingalage1.3 %. B and C were assayed for their antioxidant activity through radical scavenging activity using DPPH assay. Faculty of Science. Mean value of the weight loss of drying at 105 C0 was 11. Sri Lanka Cinnamon extracts have versatile industrial applications mainly focusing on its ability to act as an antioxidant. All the experiments were done at the research laboratory in Bandaranaike Memorial Ayurveda Research Institute (BMARI) under standard conditions. and taste of MC. acid insoluble ash content. Extracts A.4 %.9±0. The experiments were conducted in triplicate and mean values were obtained. odor. We examined extractable matter. The physicochemical properties found in MC are comparable to good characters of an ideal Choorna. LPTB Levangamage3. Studies have shown that the antioxidant activity of the cinnamon extracts is subjective to their processing conditions.6 % and acid insoluble ash content was 5.3±0. Sri Lanka 3 Department of Biosystems Technology. Rajarata University of Sri Lanka 3 Faculty of Medicine and Allied Sciences.1 % and 13 % respectively. MC can be used to treat Prameha and help with body strengthening. The linearity range for the analysis was 12. All the extracts indicated significant 222 . These parameters can be used to standardize the MC among different manufacturers. Keywords: Madhumeha Choorna.3± 0.S Thiruchenduran1. AMHS Attanayake1. particle size and organoleptic parameters such as color. SC Samarawickrama1. KRD de Silva1. W Kumbukgolla2. Faculty of Medical Sciences. PA Paranagama4 1 Interdisciplinary Center for Innovations in Biotechnology & Neurosciences. odor was characteristic and taste was pungent. However. Sri Lanka 2 Department of Biochemistry. physicochemical evaluation. The color of the samples was light brown. Cinnamon bark and oleoresins are the major raw materials for the industrial cinnamon extracts.9 %. Particle percentage which is less than 150 µm is 39. Madhumeha Choornam (MC) is a well-known Siddha drug and according to the Sidda pharmacopeia.PP 124: Assessment of physico-chemical parameters and organoleptic properties of Madhumeha Choornam LUP Liyanage1.9±1. RP Mendis1. pH value. RD Gunaratne3.6 % and the pH was 3. The aim of this study was to evaluate the physicochemical parameters of MC. total ash content. University of Sri Jayewardenepura. SS Dennis1 1 Bandaranaike Memorial Ayurveda Research Institute. Sri Lanka 4 Department of Chemistry. Prameha. The mean value of the water soluble matter and methanol soluble matter was 31. organoleptic OP 125: Assessment of the applicability of Supercritical fluid extraction method in preparing Cinnamomum zeylanicum bark oleoresins extracts based on antioxidant assays S Abira1. University of Sri Jayewardenepura.5µg/ml – 800µg/ml using butylated hydroxyl toluene (BHT) standard. TK Nawarathne1. standardization is still important to recognize the quality of the formulation. University of Kelaniya. Rajarata University of Sri Lanka The subject of herbal drug standardization is complicated because of the complex nature of herbal drugs in their composition. Sri Lanka 2 Institute of Postharvest Technology. Tripalā Cūrna. obesity. Clinically diagnosed 60 patients with Medo Roga were selected at the Kāyacikitsā clinic of Ayurvedic Teaching Hospital Borella and divided in to two by 30 subjects for each group. The BHT equivalent radical scavenging activity of the extracts at IC50 were 0. thus the contour of the body can be correlated with obesity and hyperlipidemia.5µg/ml. This study indicates that extracts from cinnamon bark oleoresins extracted using the supercritical extraction method has higher antioxidant activity compared to water extracts but an equivalent antioxidant activity with extracts from Soxhlet’s extraction. 544. Institute of Indigenous Medicine.001) and there were not significant mean differences between two groups. RDH Kulathunga2. Sri Lanka and Welagedara Ayurvedic Hospital. are higher than the mean difference in Group 1. Keywords: Medo Roga. Kurunegala. Daśāṅga Guggulu.40 respectively and 49.32 for BHT standard.035).68. Sri Lanka 3 National Hospital of Sri Lanka Medo Roga increases Kapha doṣa and Medo dhātu. University of Colombo. Statistical analysis showed that Daśāṅga Guggulu and Tripalā Cūrna showed a highly significant mean reduction of all clinical features (p<0. thus Tripalā Cūrna was more effective than Daśāṅga Guggulu for reduction of clinical features of Medo Roga.antioxidant activity against blank at concentrations above 12.039 respectively. in management of clinical features of Medo Roga. Ten clinical features of Medo Roga mentioned in text of Mādhava Nidāna were considered and Lipid Profile and BMI were calculated before and after the treatment. Extract from Soxhlet’s extraction didn’t indicate significant differences with the other two extracts at a significant level of 0. antioxidant activity.62 and 1261. IC550 values of the samples were 587.084. Even though these are major health issues in the world.74. DPPH PP 126: A comparative assessment of two Ayurvedic formulations on clinical features of Medo Roga JMGSL Jayasinghe1. Daśāṅga Guggulu and Tripalā Cūrna. The objective of this study was to assess the effectiveness of two Ayurvedic remedies comparatively. Institute of Indigenous Medicine.05 (p-values: A-B = 0. B-C = 0.091 and 0. hyperlipidemia 223 . University Of Colombo. DUS Rathnapala3 1 Department of Kayachikitsa. Daśāṅga Guggulu 3 g and Tripalā Cūrna 15 g per day were administrated for Group 1 and Group 2 respectively for three months. 0.057). But the mean difference in Group 2 for most of the clinical features. allopathic medicines are discouraged due to their inefficiency of reducing signs and symptoms. Supercritical fluid extraction exhibited significantly higher antioxidant activity when compared with water extract (p-value = 0. Sri Lanka 2 Department of Kayachikitsa. Keywords: Cinnamon. 2. Sri Lanka 3 Depatment of Pathology. Sri Lanka 2 Biochemistry. ABTS cation scavenging activity of trolox (0. University of Sri Jayewardenepura. School of Clinical Sciences. T Collet Indigenous Medicines Group. Eclipta alba. 1. UG Chandrika2. NO scavenging activity PP 128: Evaluation of the antimicrobial effects of bioactive compounds in Australian native plants V Agampodi. This project aimed to investigate the antibacterial properties of compounds present in two plant species denoted 8481 and 8482. The gallic acid equivalent phenolic content of the LLC was 1050/7g.5 billion by 2021. Institute of Health and Biomedical Innovation. Herbal remedies for the treatment of wound infections have been used for millennia across a multitude of cultures. Extracts were tested against 19 of the most common wound-colonising microflora using the well diffusion assay. total phenols. thereby modulating the healing process. AM Abeysekera1 1 Depatment of Chemistry. Terminalia chebula. P. Antibiotic discs were used as the positive control whilst sterile MilliQ water acted as the negative control.2-azinobis-ethylbenz-thiazoline-6-sulphonic acid (ABTS) and Nitric oxide (NO) scavenging activity. These results show that the antioxidant capacity of the extract of LLC is low compared to the standards. Phyllanthus amarus. T. As such. Queensland University of Technology. bellerica. Trolox the standard showed 97% scavenging activity at 1 μg/ml whereas LLC showed 55% scavenging activity at 1000 μg/ml. ABTS. C Padumadasa1. Australia Chronic wounds have a severe socioeconomic impact on the cost of health care and are predicted to rise substantially in the future with concomitant management costs expected to exceed $18. Keywords: hepatoprotective formulation.PP 127: Antioxidant activity of Linklive CareTM: A hepatoprotective polyherbal formulation KAAU Karunarathna1. DPPH. Osbekia octandra. there is an increased interest in plant-derived compounds which have the potential to inhibit wound-colonising bacteria. University of Sri Jayewardenepura. Results 224 . This indicates that anti-oxidant effects do not play an important role in the reported hepatoprotective activity of LLC. Tephrosia purpurea and Vernonia cinerea was formulated by an expert panel of Ayurvedic physicians at Link Natural Product (Pvt) limited. B Senevirathna3. All experiments were performed in triplicate and repeated three times.125 - 2 μg/ml) and LLC (50. Quercetin (25 -400 μg/ ml) and the extract of LLC (50 -1000 μg/ ml) showed a dose dependent elevation in NO scavenging activity. except in the NO scavenging activity assay. At the same concentration the extract of LLC showed 50 % NO scavenging activity respectively. Glycyrrhiza glabra. Tinospora codifolia. Bacterial inhibition was determined by measuring the radius of the inhibition zone. Piper longum. Curcuma longa. emblica.respectively. DPPH scavenging activity of the extract of LLC (10 -300 μg/ml) and gallic acid (2 -10 μg/ml) exhibited dose dependent activity with IC50 values of 50 μg/ ml and 5 μg/ ml . Boerrhavia diffusa. Quercetin the positive control reached 58 % at 400 μg/ ml.1-diphenyl-2-picrylhydrazyl (DPPH).1000 μg/ml) also showed dose dependent scavenging activity. Sri Lanka Link LivecareTM (LLC): a new hepatoprotective polyherbal formulation that consists of 14 herbs: Andrographis paniculata. The antioxidant capacity of LLC was evaluated via total phenol content (Folin–Ciocalteu method). University of Sri Jayewardenepura. Ground plant leaves were extracted separately with methanol and water. showed that plant extracts from both species were bactericidal against eight Gram-positive bacteria including Staphylococcus aureus, Staphylococcus epidermidis, Bacillus cereus and Bacillus subtilis. Since plants 8481 and 8482 contain bioactive compounds which have been shown to elicit bactericidal activity against several common wound-colonising bacteria, ultimately, the reduction in microbial load may augment the wound healing process. The real world implication of this research is expected to produce novel plant-based therapeutics which can significantly enhance the healing of chronic wounds, thus globally reducing the socioeconomic burden. Keywords: bioactive compounds, antimicrobial, wound healing PP 129: Conceptual assessment of Lekhaniya vasti in the management of hyperlipidemia AS Nishshanka1, RDH Kulathunga2 1 Bandaranayke memorial Ayurveda research institute, Nawinne, Sri Lanka 2 Department of Kaya Chikitsa Institute of indigenous Medicine, University of Colombo, Rajagiriya, Sri Lanka Hyperlipidemia is a common lipid disorder characterized with increased serum cholesterol and serum triglyceride level. Typically it is not showing clinical symptoms. But it leads to complicated serious illnesses such as heart diseases or cerebral vascular diseases. According to the world health organization a third of ischemic heart disease is attributable to high cholesterol. Raised serum cholesterol is estimated to cause 2.6 million deaths and 29.7million disability adjusted life years. Hyperlipidemia can be correlated with medo vriddhi as well as dhamani pratichaya which has been emphasized in Charaka samhita under kaphaja diseases. According to Ayurveda, Lekhana property has a potential in reducing both kapha and medas in the body. Further Susruta Samhita mentioned that vasti, has an ability to spread its potency throughout the body. Vasti is doing srotas shodhana and alleviating vata. These are important for the process of samprapti khandana in medo vriddhi. Therefore it is important to review lekhaniya vasti as a remedy for hyperlipidemia which mainly caused by kapha and medas along with vitiated vata dosha. Lekhana dravyas show khara, tikshna, ushna, laghu guna; katu tikta rasa; katu vipaka and ushna virya due to its akasha, vayu and agni bootic composition. Based on these facts logical inference can be drawn, lekhaniya vasti should be very effective in the management of hyperlipidemia in preventive and curative aspect both. Niruha of karanja Kashaya and Anuvasana of madanaphala taila mentioned in charaka samhita can be used here in the form of kala vasti. Keywords: hyperlipidemia, kapha, medas, medo vriddhi, Lekhaniya vasti PP 130: Clinical Evaluation of Cholesterol Lowering Effect of Sihinmidi (Premna integrifolia Linn) Decoction KDW De Silva, SD Hapuarachchi, SKMK Herapathdeniya Department of Dravyagunavignana, Institute of Indigenous Medicine, University of Colombo, Sri Lanka Sihinmidi (Premna integrifolia – Verbenaceae) commonly known as (Agnimanta) and traditional physicians used the decoction of this root for obesity and hyperlipidemea in folk medical practice in Sri Lanka. This study was designed to evaluate the therapeutic effectiveness of Sihinmidi. The objective of this study was to clinically evaluate the cholesterol lowering effect of Sihinmidi 225 decoction. Thirty patients (age 18 – 60 yrs) of both sexes (BMI >30 Kg/m2) were selected randomly from the OPD at Ayurveda hospitals in Borella and Molligoda. They were investigated and the values of serum cholesterol and serum triglyceride were recorded before and after treatments. Thirty patients were treated with 120 ml of Sihinmidi decoction twice daily for four weeks. After four weeks of treatment, the study group showed a (p = 0.00) significant reduction of the values of serum cholesterol and showed (p = 0.003) significant reduction of the values of serum triglyceride. The study suggests that Sihinmidi decoction can be used as a cholesterol lowering agent for hyperlipidemea. Keywords: cholesterol lowering effect, Sihinmiidi, Premna integrifolia PP 131: A systematic review on medicinal plants used to treat diabetes mellitus FS Rafeek, V Paheerathan, R Piratheepkumar Unit of Siddha Medicine, Trincomalee Campus, Eastern University, Sri Lanka Aim of the present study is evaluated various medicinal plants used for anti-diabetic activity. Diabetes mellitus is one of the most common non-communicable diseases globally. It is the fourth leading causes of death in the most developed countries and there in substantial evidenced that it in epidemic in many developing and newly industrialized nations. This is posing a serious threat to be met within 21st century. Since ancient time plants have been exemplary source of medicine. The present study was conducted to bring up to date the scientific evidence on the 39 plants, collected from different areas of Trincomalee district, widely used by people in the condition of Diabetes mellitus. Among the 39 plants 35 plant species were possessed anti-diabetic activity. The total plants belonging to 35 species were described and listed in the review. Required papers about diabetes and effective plants were searched from the databases, including Science direct, PubMed, Wiley, Scopus, and Springer. Keywords in this study are ”medicinal plants”, “diabetes”, “symptom”, “herbal”, and “treatment”. The plants have been tabulated with botanical, family and medicinal uses. All the plants which had Anti-diabetic activity were investigated according to this review. Although limited information was obtained on the Anti-diabetic activities of these plants, it is however foreseen that government institutes and scientific communities will pay more attention to these plants in future research. This study led to the wealth knowledge of medicinal plants that are being used to treat Diabetes mellitus. The further investigation on these medicinal plants may lead to the discovery of active chemical composition which helps to maintain the Diabetes mellitus in control. Keywords: Diabetes mellitus, medicinal plants, anti-diabetic activity 226 Committee of steering 1. Chief Patron Hon. Rajitha Senarathne Minister of Health Nutrition & Indigenous Medicine, Sri Lanka. 2. Patron Hon. Faizal Cassim Deputy Minister of Health Nutrition & Indigenous Medicine, Sri Lanka. 3. Chief Executive Officer Mr. Janaka Sugathadasa Ministry of Health, Nutrition and Indigenous Medicine, Sri Lanka. 4. Project Co-Chair Prof. Ranil de Silva Director, World Class University Project, Director, Interdisciplinary Center for Innovations in Biotechnology and Neurosciences, Associate Professor, Department of Anatomy, Faculty of Medical Sciences, University of Sri Jayewardenepura 5. Project Co-Chair Mr. S. Janaka Sri Chandraguptha Additional Secretary (Development), Ministry of Health, Nutrition and Indigenous Medicine, Sri Lanka 6. Project Director Mrs. Mangala Karunathilake Commissioner of Ayurveda, Department of Ayurveda, Sri Lanka. 7. Project Secretary Dr. (Mrs) Rameeladevi Thatparan Ayurveda Medical Officer Bandaranayake Memorial Ayurveda Research Institute Nawinna, Maharagama, Sri Lanka. 8. Co-Chair of Symposium Dr.(Mrs) Nalika Sepali Gunawardena National Professional Officer (Health Systems Analysis and Evidence), World Health Organization Country Office for Sri Lanka. 9. Co-Chair of Symposium Dr. S M S Samarakoon Senior Lecturer, Institute of Indigenous Medicine, University of Colombo, Rajagiriya , Sri Lanka. 10. Project Operation Executive Dr. Dammika Abeygunawardena Provincial Commissioner of Ayurveda – Central Province, Kandy, Sri Lanka. 227 Media Coordination Committee Victuals Committee Fund raising committee International relationship committee Mr. Lakshmi Kumari Dr. Kumari Kumarasekara Dr. Dammika AbAbeygunawardena Dr. Allan Ludowyke Mr. Prasanna Adikari Dr. Swarna Kaluthotage Dr. Jeevani Thiranagama Dr. Thilina Wannigasegara Dr. Nalaka Rangana Dr. Nanda Dadallage Dr. Wasantha Basnayaka Dr. Nadeeja Herath Dr. Senaka Kodikara Dr. Indika Jayalal Dr. Manoj Medagoda Mrs. Savithri Gunathilaka Dr. Manori Ranaweera Dr. Udaya Subasingha Symposium organizing Committee Printing committee Dr. Suleka Punchihewa Dr. Swarna Kaluthotage Dr. Krishanthi Jeevani Dr. Danister L. Perera Dr. Ajith Kumara Dr. Pushpa Madawa Dr. Nayana Liyanege Dr. Senaka Kodikara Inaugural and Valedictory Session Technical Evaluation Committee Dr. Champika Peiris Organizing Committee Dr. Pushpika Jayasundera Publication committee Mr. Janaka Chandragupta Dr. Gayani Wijesuriya Dr. Vishaka Srikantha Mrs.Mangala.Gunathilaka Dr. Dammika Abeyagunawardana Dr. Sumudu Uduwanage Dr. Saumya Janakanthi Kumari Dr. Dammika Abeyagunawardana Dr. Upul Weerawardana Dr. Sripriya Sugitharan Dr. Nadi Gayani Perera Dr. Thilina Wanigasegara Dr. Wasantha Padmakumara Dr. Nadeeka Perera Dr. Rameeladevi.Thatparan Pre-Symposium organizing Dr. Priyanga Lambias Dr. Fasmiya Joonus Mrs. Savithri Gunathilaka commityee Dr. Amila Withanage Dr. Sudarma Umayangani Dr. Rishad Muhari Financial management committee Scientific sessions organizing Prof.Ranil de Silva Dr. Madhavi Perera committee Dr. Sanseevan Harichandran Dr. Thushari Senarathna Mr. P. Dayashantha Prof. Ranil De Silwa Mr. Nalaka Wijekoon, Dr. Kethakie Kulapala Mr. S. A. Dharmawardana Prof. Nalika Gunawardana Mr. Lakmal Gonawala, Dr. Sampath Attanayake Dr. Dammika Abeyagunawardena Prof. Priyani Paranagama Mr. Yoonus Imran, Dr. Nadeeka Promodani Mr. K. P. Yogachandra Dr. Swarna Hapuarachchi Ms. Sanduni Jayaweera Ms.Chamindri Nagasena Miss. Dinesha Lasanthi Dr. Senaka Pilapitiya Mr. Ashan Dayananda, Mrs Dulanjali Fernando Dr. Kumari Kumarasekara Dr. Chayanika Padumadasa, Exhibition and Trade fair organizing Dr. Thilina Wanigasekera Dr. Helani Munasinghe, committee Dr. Priyani Peiris Dr Cuda Meedeniya Dr. Dammika Abeygunawardena Dr S.M.S Samarakoon Ms. Chamindri Nagasena Dr. Jeevani Thiranagama Logistic and Transport committee Dr. Parakrama Hemachandra Dr. Nanda Dadallage Dr. Wasantha Basnayaka Dr. Danister Perera Dr. Manoj Laxman Dr. Parakrama Amaratunga Dr. Ajith Kumara Dr. Udaya Subasingha Dr. Thrinath Thilanjaya Dr. Cassim Riaz Mrs. Savithri Gunathilaka 228 229 .